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RGS2 (Homo sapiens) in pLenti6.2/V5-DEST (V5-tagged lentiviral mammalian expression vector)

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Explanation of Terms


Gene: Gene Symbol:  RGS2
Gene Name:  regulator of G-protein signaling 2, 24kDa
Sequence             
Original Clone ID: FLH269105.01L
Keyword: None
Species: Homo sapiens
Type: cDNA
Vector Name: pLenti6.2/V5-DEST               Format:  FUSION
Source: HIP
Description: None
Comments: None
Mutation/
Discrepancy :
No / No
Publications: None
Authors:
HsCD00329603

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AVAILABLE
No restriction
Special MTA: None

 

Insert sequence: 636nts         Open reading frame : 1 to 636
Reference Sequence Alignment

ATGCAAAGTGCTATGTTCTTGGCTGTTCAACACGACTGCAGACCCATGGACAAGAGCGCA
GGCAGTGGCCACAAGAGCGAGGAGAAGCGAGAAAAGATGAAACGGACCCTTTTAAAAGAT
TGGAAGACCCGTTTGAGCTACTTCTTACAAAATTCCTCTACTCCTGGGAAGCCCAAAACC
GGCAAAAAAAGCAAACAGCAAGCTTTCATCAAGCCTTCTCCTGAGGAAGCACAGCTGTGG
TCAGAAGCATTTGACGAGCTGCTAGCCAGCAAATATGGTCTTGCTGCATTCAGGGCTTTT
TTAAAGTCGGAATTCTGTGAAGAAAATATTGAATTCTGGCTGGCCTGTGAAGACTTCAAA
AAAACCAAATCACCCCAAAAGCTGTCCTCAAAAGCAAGGAAAATATATACTGACTTCATA
GAAAAGGAAGCTCCAAAAGAGATAAACATAGATTTTCAAACCAAAACTCTGATTGCCCAG
AATATACAAGAAGCTACAAGTGGCTGCTTTACAACTGCCCAGAAAAGGGTATACAGCTTG
ATGGAGAACAACTCTTATCCTCGTTTCTTGGAGTCAGAATTCTACCAGGACTTGTGTAAA
AAGCCACAAATCACCACAGAGCCTCATGCTACATTG


Coding Sequence Details
Insert Sequence Verified?: N Verification Method: Insert was fully sequenced in parent vector
Reference Sequence Annotations:
End on reference sequence 668
Start on reference sequence 33
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
5p GTACAAAAAAGCAGGCTCCACC(atg-goi)

3' Linker Sequence:
5p (goi-stop)GACCCAGCTTTCTTGTAC

 

 Recommended Growth Condition:

Distributed in bacterial strain : DH5-alpha T1 phage resistant
Antibiotic Selection: Host Type:  bacterial    Marker:  ampicillin
Host Type:  mammalian    Marker:  blasticidin
Bacterial Selection Condition:  100 ug/mL ampicillin
Growth Condition:  Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:  Commonly used conditions for ampicillin resistant plasmid clones.
Protein Expression Results: None
Recommended expression in: Not Applicable
       DyNA Vector Map

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Vector Name: pLenti6.2/V5-DEST

pLenti6.2V5-DEST in advanced viewed

Synonyms: pLenti6.2/V5-DEST
Sequencing Primer: Forward:  CMV forward
Reverse:  V5 reverse
Description: Lentiviral mammalian expression vector;CMV promoter and C-terminal V5 tag; ampicillin resistance in bacteria; blasticidin resistance in mammalian cells; Gateway recombinational cloning.
Comments: None
Size (bp): 8813
Parent Vector: None
Empty Vector: None
Properties: Gateway, acceptor (destination), lentiviral infection, mammalian expression, recombinational cloning, with tag/fusion/marker
Author Name: Invitrogen
Publications: None
Vector Map:         Vector Sequence:
Sequence:

 



Vector Features:

Type Name Description Start Position End Position
bacterial origin pUC ori pUC origin of replication 7733 8406
death cassette ccdB ccdB death cassette (removed when insert is present) 3767 3976
packaging signal HIV-1 psi HIV-1 psi packaging signal 521 565
poly-A signal SV40 SV40 polyadenylation signal 5639 5770
promoter RSV RSV/5' LTR hybrid promoter 1 410
promoter CMV CMV promoter driving gene of interest 1809 2392
promoter PGK PGK promoter to drive selection marker in mammalian cells 4266 4771
recombination site attR2 attR2 recombinational cloning site 4127 4142
recombination site attR1 attR1 recombinational cloning site 2440 2456
response element HIV-1 RRE HIV-1 Rev response element (RRE) 1075 1308
selectable marker AmpR Ampicillin resistance gene for selection in bacteria 6728 7588
selectable marker BlastR Blasticidin resistance gene in mammalian cells 4849 5249
selectable marker CmR chloramphenicol resistance marker (removed in plasmids with insert) 2673 3329
splice acceptor site 3' splice acceptor two 3' splice acceptors at 1656 and 1684 1656 1656
splice donor site 5' splice donor 5' splice donor 520 520
tag V5 C-terminal V5 epitope tag 4197 4238

 

  • Gene
  • Pathways
  • Protein
  • Clone
  • Organism
  • Genome
  • Reagents

Gene

 

Gene Symbol : RGS2
Symbol Nomenclature : RGS2
SYNONYM : G0S8
Designation : G0 to G1 switch regulatory 8, 24kD|G0/G1 switch regulatory protein 8|cell growth-inhibiting gene 31 protein|cell growth-inhibiting protein 31|regulator of G-protein signaling 2
Full Nomenclature : regulator of G-protein signaling 2, 24kDa
GENEID : 5997
Locus Tag : GIG31
HGNC : 9998
MIM : 600861
Vega : OTTHUMG00000035600
Target GenBank: BC007049

 

Reference Sequence Alignment

  

HsCD00329603      ATGCAAAGTGCTATGTTCTTGGCTGTTCAACACGACTGCAGACCCATGGACAAGAGCGCA
NM_002923.3       ATGCAAAGTGCTATGTTCTTGGCTGTTCAACACGACTGCAGACCCATGGACAAGAGCGCA
                  ************************************************************

HsCD00329603      GGCAGTGGCCACAAGAGCGAGGAGAAGCGAGAAAAGATGAAACGGACCCTTTTAAAAGAT
NM_002923.3       GGCAGTGGCCACAAGAGCGAGGAGAAGCGAGAAAAGATGAAACGGACCCTTTTAAAAGAT
                  ************************************************************

HsCD00329603      TGGAAGACCCGTTTGAGCTACTTCTTACAAAATTCCTCTACTCCTGGGAAGCCCAAAACC
NM_002923.3       TGGAAGACCCGTTTGAGCTACTTCTTACAAAATTCCTCTACTCCTGGGAAGCCCAAAACC
                  ************************************************************

HsCD00329603      GGCAAAAAAAGCAAACAGCAAGCTTTCATCAAGCCTTCTCCTGAGGAAGCACAGCTGTGG
NM_002923.3       GGCAAAAAAAGCAAACAGCAAGCTTTCATCAAGCCTTCTCCTGAGGAAGCACAGCTGTGG
                  ************************************************************

HsCD00329603      TCAGAAGCATTTGACGAGCTGCTAGCCAGCAAATATGGTCTTGCTGCATTCAGGGCTTTT
NM_002923.3       TCAGAAGCATTTGACGAGCTGCTAGCCAGCAAATATGGTCTTGCTGCATTCAGGGCTTTT
                  ************************************************************

HsCD00329603      TTAAAGTCGGAATTCTGTGAAGAAAATATTGAATTCTGGCTGGCCTGTGAAGACTTCAAA
NM_002923.3       TTAAAGTCGGAATTCTGTGAAGAAAATATTGAATTCTGGCTGGCCTGTGAAGACTTCAAA
                  ************************************************************

HsCD00329603      AAAACCAAATCACCCCAAAAGCTGTCCTCAAAAGCAAGGAAAATATATACTGACTTCATA
NM_002923.3       AAAACCAAATCACCCCAAAAGCTGTCCTCAAAAGCAAGGAAAATATATACTGACTTCATA
                  ************************************************************

HsCD00329603      GAAAAGGAAGCTCCAAAAGAGATAAACATAGATTTTCAAACCAAAACTCTGATTGCCCAG
NM_002923.3       GAAAAGGAAGCTCCAAAAGAGATAAACATAGATTTTCAAACCAAAACTCTGATTGCCCAG
                  ************************************************************

HsCD00329603      AATATACAAGAAGCTACAAGTGGCTGCTTTACAACTGCCCAGAAAAGGGTATACAGCTTG
NM_002923.3       AATATACAAGAAGCTACAAGTGGCTGCTTTACAACTGCCCAGAAAAGGGTATACAGCTTG
                  ************************************************************

HsCD00329603      ATGGAGAACAACTCTTATCCTCGTTTCTTGGAGTCAGAATTCTACCAGGACTTGTGTAAA
NM_002923.3       ATGGAGAACAACTCTTATCCTCGTTTCTTGGAGTCAGAATTCTACCAGGACTTGTGTAAA
                  ************************************************************

HsCD00329603      AAGCCACAAATCACCACAGAGCCTCATGCTACATTG
NM_002923.3       AAGCCACAAATCACCACAGAGCCTCATGCTACATGA
                  ********************************** .

Pathways

Panther : Heterotrimeric G-protein signaling pathway-Gi alpha and Gs alpha mediated pathway
Panther : Heterotrimeric G-protein signaling pathway-Gq alpha and Go alpha mediated pathway
Reactome : G alpha (q) signalling events
Reactome : GPCR downstream signaling
Reactome : Gastrin-CREB signalling pathway via PKC and MAPK
Reactome : Signal Transduction
Reactome : Signaling by GPCR
WikiPathway : Calcium Regulation in the Cardiac Cell
WikiPathway : Myometrial Relaxation and Contraction Pathways

Protein

SMART domain : RGS : Regulator of G protein signalling domain
UniProt : P41220
HPRD : 02917

Clone

Cloning Information : 269105
HIP Master Clone ID : 169394
Original Clone ID : FLH269105.01L

Organism


TAX_ID : 9606
Species Specific ID: 5997

Genome

Chromosome : 1
Map Location : 1q31
Ensembl : ENSG00000116741

Reagents

Labome : RGS2-antibody