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LASS4 (Homo sapiens) in pLenti6.2/V5-DEST (V5-tagged lentiviral mammalian expression vector)

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Explanation of Terms


Gene: Gene Symbol:  CERS4
Gene Name:  ceramide synthase 4
Sequence             
Original Clone ID: FLH269106.01L
Keyword: None
Species: Homo sapiens
Type: cDNA
Vector Name: pLenti6.2/V5-DEST               Format:  FUSION
Source: HIP
Description: None
Comments: None
Mutation/
Discrepancy :		 No / No
Publications: None
Authors:
HsCD00329604

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AVAILABLE
No restriction
Special MTA: None

 

Insert sequence: 1185nts         Open reading frame : 1 to 1185
Reference Sequence Alignment 

ATGCTGTCCAGTTTCAACGAGTGGTTTTGGCAGGACAGGTTCTGGTTACCACCCAATGTC
ACGTGGACAGAGCTAGAAGACCGGGATGGCCGTGTCTACCCCCACCCCCAGGACTTGTTG
GCAGCCCTGCCCCTGGCGCTGGTCCTCCTGGCCATGCGCCTTGCCTTTGAGAGATTCATT
GGCCTGCCCCTGAGCCGGTGGCTGGGTGTGAGGGATCAGACCAGGAGGCAAGTGAAGCCC
AACGCCACGCTGGAGAAACACTTCCTCACGGAAGGGCACAGGCCCAAGGAGCCCCAGCTG
TCTCTCCTGGCCGCCCAGTGTGGCCTCACGCTGCAGCAGACCCAGCGATGGTTCCGGAGA
CGCCGGAACCAGGATCGACCCCAGCTGACCAAGAAGTTCTGTGAGGCCAGCTGGAGGTTT
CTCTTCTACCTGTCCTCCTTCGTGGGCGGCCTCTCGGTCCTGTACCACGAGTCATGGCTG
TGGGCACCAGTAATGTGCTGGGACAGGTACCCAAACCAGACTCTGAAGCCATCCCTGTAC
TGGTGGTACCTCTTGGAGCTGGGTTTCTACCTCTCACTGCTAATCAGGCTGCCCTTTGAT
GTCAAGCGCAAGGATTTCAAGGAGCAGGTGATACACCACTTCGTGGCGGTCATCCTGATG
ACCTTCTCCTACAGTGCCAACCTGCTGCGCATTGGCTCTCTGGTGCTGCTGTTACATGAT
TCCTCTGACTACCTGCTGGAGGCCTGTAAGATGGTCAACTACATGCAGTATCAGCAAGTG
TGCGACGCTCTCTTCCTCATCTTCTCCTTTGTCTTCTTCTACACCCGACTGGTCCTCTTT
CCCACCCAGATCCTCTACACCACATACTACGAGTCCATCAGCAACAGGGGCCCCTTCTTC
GGCTACTACTTCTTCAACGGGCTTCTGATGTTGCTGCAGCTGCTGCACGTGTTCTGGTCT
TGCCTCATTCTGCGCATGCTCTATAGCTTCATGAAGAAGGGCCAGATGGAGAAGGACATT
CGTAGTGATGTAGAAGAATCAGACTCCAGTGAGGAGGTGGCGGCGGCCCAGGAACCTCTG
CAGCTAAAGAACGGGGCAGCTGGAGGGCCCAGGCCAGCCCCCACTGATGGCCCTCAGAGC
CGGGTGGCCGGGCGTCTGACCAACAGGCACACAACAGCCACATTG

Coding Sequence Details
Insert Sequence Verified?: N Verification Method: Insert was fully sequenced in parent vector
Reference Sequence Annotations:
End on reference sequence 1317
Start on reference sequence 133
Mutations:
None 		Discrepancy:
None
5' Linker Sequence: 
5p GTACAAAAAAGCAGGCTCCACC(atg-goi)
3' Linker Sequence: 
5p (goi-stop)GACCCAGCTTTCTTGTAC

 

 Recommended Growth Condition:

Distributed in bacterial strain : DH5-alpha T1 phage resistant
Antibiotic Selection: Host Type:  bacterial    Marker:  ampicillin
Host Type:  mammalian    Marker:  blasticidin
Bacterial Selection Condition:  100 ug/mL ampicillin
Growth Condition:  Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:  Commonly used conditions for ampicillin resistant plasmid clones.
Protein Expression Results: None
Recommended expression in: Not Applicable
       DyNA Vector Map

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Vector Name: pLenti6.2/V5-DEST

pLenti6.2V5-DEST in advanced viewed
Synonyms: pLenti6.2/V5-DEST
Sequencing Primer: Forward:  CMV forward
Reverse:  V5 reverse
Description: Lentiviral mammalian expression vector;CMV promoter and C-terminal V5 tag; ampicillin resistance in bacteria; blasticidin resistance in mammalian cells; Gateway recombinational cloning.
Comments: None
Size (bp): 8813
Parent Vector: None
Empty Vector: None
Properties: Gateway, acceptor (destination), lentiviral infection, mammalian expression, recombinational cloning, with tag/fusion/marker
Author Name: Invitrogen
Publications: None
Vector Map:         Vector Sequence:
Sequence:

 



Vector Features:

Type Name Description Start Position End Position
bacterial origin pUC ori pUC origin of replication 7733 8406
death cassette ccdB ccdB death cassette (removed when insert is present) 3767 3976
packaging signal HIV-1 psi HIV-1 psi packaging signal 521 565
poly-A signal SV40 SV40 polyadenylation signal 5639 5770
promoter RSV RSV/5' LTR hybrid promoter 1 410
promoter CMV CMV promoter driving gene of interest 1809 2392
promoter PGK PGK promoter to drive selection marker in mammalian cells 4266 4771
recombination site attR2 attR2 recombinational cloning site 4127 4142
recombination site attR1 attR1 recombinational cloning site 2440 2456
response element HIV-1 RRE HIV-1 Rev response element (RRE) 1075 1308
selectable marker AmpR Ampicillin resistance gene for selection in bacteria 6728 7588
selectable marker BlastR Blasticidin resistance gene in mammalian cells 4849 5249
selectable marker CmR chloramphenicol resistance marker (removed in plasmids with insert) 2673 3329
splice acceptor site 3' splice acceptor two 3' splice acceptors at 1656 and 1684 1656 1656
splice donor site 5' splice donor 5' splice donor 520 520
tag V5 C-terminal V5 epitope tag 4197 4238

 

  • Gene
  • Pathways
  • Protein
  • Clone
  • Organism
  • Genome
  • Reagents

Gene

 

Gene Symbol : CERS4
Symbol Nomenclature : CERS4
SYNONYM : LASS4; Trh1
Designation : LAG1 homolog, ceramide synthase 4|LAG1 longevity assurance homolog 4
Full Nomenclature : ceramide synthase 4
GENEID : 79603
HGNC : 23747
MIM : 615334
Vega : OTTHUMG00000172570
Target GenBank: BC009828

 

Reference Sequence Alignment

  

HsCD00329604      ATGCTGTCCAGTTTCAACGAGTGGTTTTGGCAGGACAGGTTCTGGTTACCACCCAATGTC
NM_024552.2       ATGCTGTCCAGTTTCAACGAGTGGTTTTGGCAGGACAGGTTCTGGTTACCACCCAATGTC
                  ************************************************************

HsCD00329604      ACGTGGACAGAGCTAGAAGACCGGGATGGCCGTGTCTACCCCCACCCCCAGGACTTGTTG
NM_024552.2       ACGTGGACAGAGCTAGAAGACCGGGATGGCCGTGTCTACCCCCACCCCCAGGACTTGTTG
                  ************************************************************

HsCD00329604      GCAGCCCTGCCCCTGGCGCTGGTCCTCCTGGCCATGCGCCTTGCCTTTGAGAGATTCATT
NM_024552.2       GCAGCCCTGCCCCTGGCGCTGGTCCTCCTGGCCATGCGCCTTGCCTTTGAGAGATTCATT
                  ************************************************************

HsCD00329604      GGCCTGCCCCTGAGCCGGTGGCTGGGTGTGAGGGATCAGACCAGGAGGCAAGTGAAGCCC
NM_024552.2       GGCCTGCCCCTGAGCCGGTGGCTGGGTGTGAGGGATCAGACCAGGAGGCAAGTGAAGCCC
                  ************************************************************

HsCD00329604      AACGCCACGCTGGAGAAACACTTCCTCACGGAAGGGCACAGGCCCAAGGAGCCCCAGCTG
NM_024552.2       AACGCCACGCTGGAGAAACACTTCCTCACGGAAGGGCACAGGCCCAAGGAGCCCCAGCTG
                  ************************************************************

HsCD00329604      TCTCTCCTGGCCGCCCAGTGTGGCCTCACGCTGCAGCAGACCCAGCGATGGTTCCGGAGA
NM_024552.2       TCTCTCCTGGCCGCCCAGTGTGGCCTCACGCTGCAGCAGACCCAGCGATGGTTCCGGAGA
                  ************************************************************

HsCD00329604      CGCCGGAACCAGGATCGACCCCAGCTGACCAAGAAGTTCTGTGAGGCCAGCTGGAGGTTT
NM_024552.2       CGCCGGAACCAGGATCGACCCCAGCTGACCAAGAAGTTCTGTGAGGCCAGCTGGAGGTTT
                  ************************************************************

HsCD00329604      CTCTTCTACCTGTCCTCCTTCGTGGGCGGCCTCTCGGTCCTGTACCACGAGTCATGGCTG
NM_024552.2       CTCTTCTACCTGTCCTCCTTCGTGGGCGGCCTCTCGGTCCTGTACCACGAGTCATGGCTG
                  ************************************************************

HsCD00329604      TGGGCACCAGTAATGTGCTGGGACAGGTACCCAAACCAGACTCTGAAGCCATCCCTGTAC
NM_024552.2       TGGGCACCAGTAATGTGCTGGGACAGGTACCCAAACCAGACTCTGAAGCCATCCCTGTAC
                  ************************************************************

HsCD00329604      TGGTGGTACCTCTTGGAGCTGGGTTTCTACCTCTCACTGCTAATCAGGCTGCCCTTTGAT
NM_024552.2       TGGTGGTACCTCTTGGAGCTGGGTTTCTACCTCTCACTGCTAATCAGGCTGCCCTTTGAT
                  ************************************************************

HsCD00329604      GTCAAGCGCAAGGATTTCAAGGAGCAGGTGATACACCACTTCGTGGCGGTCATCCTGATG
NM_024552.2       GTCAAGCGCAAGGATTTCAAGGAGCAGGTGATACACCACTTCGTGGCGGTCATCCTGATG
                  ************************************************************

HsCD00329604      ACCTTCTCCTACAGTGCCAACCTGCTGCGCATTGGCTCTCTGGTGCTGCTGTTACATGAT
NM_024552.2       ACCTTCTCCTACAGTGCCAACCTGCTGCGCATTGGCTCTCTGGTGCTGCTGTTACACGAT
                  ******************************************************** ***

HsCD00329604      TCCTCTGACTACCTGCTGGAGGCCTGTAAGATGGTCAACTACATGCAGTATCAGCAAGTG
NM_024552.2       TCCTCTGACTACCTGCTGGAGGCCTGTAAGATGGTCAACTACATGCAGTATCAGCAAGTG
                  ************************************************************

HsCD00329604      TGCGACGCTCTCTTCCTCATCTTCTCCTTTGTCTTCTTCTACACCCGACTGGTCCTCTTT
NM_024552.2       TGCGACGCTCTCTTCCTCATCTTCTCCTTTGTCTTCTTCTACACCCGACTGGTCCTCTTT
                  ************************************************************

HsCD00329604      CCCACCCAGATCCTCTACACCACATACTACGAGTCCATCAGCAACAGGGGCCCCTTCTTC
NM_024552.2       CCCACCCAGATCCTCTACACCACATACTACGAGTCCATCAGCAACAGGGGCCCCTTCTTC
                  ************************************************************

HsCD00329604      GGCTACTACTTCTTCAACGGGCTTCTGATGTTGCTGCAGCTGCTGCACGTGTTCTGGTCT
NM_024552.2       GGCTACTACTTCTTCAACGGGCTTCTGATGTTGCTGCAGCTGCTGCACGTGTTCTGGTCT
                  ************************************************************

HsCD00329604      TGCCTCATTCTGCGCATGCTCTATAGCTTCATGAAGAAGGGCCAGATGGAGAAGGACATT
NM_024552.2       TGCCTCATTCTGCGCATGCTCTATAGCTTCATGAAGAAGGGCCAGATGGAGAAGGACATT
                  ************************************************************

HsCD00329604      CGTAGTGATGTAGAAGAATCAGACTCCAGTGAGGAGGTGGCGGCGGCCCAGGAACCTCTG
NM_024552.2       CGTAGTGATGTAGAAGAATCAGACTCCAGTGAGGAGGCGGCGGCGGCCCAGGAACCTCTG
                  ************************************* **********************

HsCD00329604      CAGCTAAAGAACGGGGCAGCTGGAGGGCCCAGGCCAGCCCCCACTGATGGCCCTCAGAGC
NM_024552.2       CAGCTAAAGAACGGGGCAGCTGGAGGGCCCAGGCCAGCCCCCACTGATGGCCCTCGGAGC
                  *******************************************************.****

HsCD00329604      CGGGTGGCCGGGCGTCTGACCAACAGGCACACAACAGCCACATTG
NM_024552.2       CGGGTGGCCGGGCGTCTGACCAACAGGCACACAACAGCCACATAG
                  *******************************************:*

Pathways

Reactome : Metabolism
Reactome : Metabolism of lipids and lipoproteins
Reactome : Sphingolipid de novo biosynthesis
Reactome : Sphingolipid metabolism

Protein

SMART domain : HOX : Homeodomain
SMART domain : TLC : TRAM, LAG1 and CLN8 homology domains.
UniProt : Q9HA82
UniProt : Q53HF9
HPRD : 13962

Clone

Cloning Information : 269106
HIP Master Clone ID : 170021
Original Clone ID : FLH269106.01L

Organism


TAX_ID : 9606
Species Specific ID: 79603

Genome

Chromosome : 19
Map Location : 19p13.2
Ensembl : ENSG00000090661

Reagents

Labome : CERS4-antibody