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DCP1A (Homo sapiens) in pLenti6.2/V5-DEST (V5-tagged lentiviral mammalian expression vector)

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Explanation of Terms


Gene: Gene Symbol:  DCP1A
Gene Name:  decapping mRNA 1A
Sequence             
Original Clone ID: FLH269112.01L
Keyword: None
Species: Homo sapiens
Type: cDNA
Vector Name: pLenti6.2/V5-DEST               Format:  FUSION
Source: HIP
Description: None
Comments: None
Mutation/
Discrepancy :
No / No
Publications: None
Authors:
HsCD00329610

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AVAILABLE
No restriction
Special MTA: None

 

Insert sequence: 1749nts         Open reading frame : 1 to 1749

ATGGAGGCGCTGAGTCGAGCTGGGCAGGAGATGAGCCTAGCGGCCCTGAAGCAACACGAC
CCCTATATCACCAGCATCGCAGACCTCACGGGCCAGGTCGCTCTGTACACCTTCTGCCCC
AAGGCCAACCAGTGGGAGAAGACTGATATAGAAGGGACCTTATTCGTATATCGAAGGTCA
GCTTCCCCTTACCATGGTTTTACCATTGTGAATCGACTAAATATGCACAATCTAGTTGAA
CCAGTGAATAAAGATTTGGAATTTCAGCTCCATGAACCATTTCTTCTGTATAGAAATGCA
AGCTTGTCGATATATAGTATCTGGTTTTATGACAAGAATGACTGTCACCGCATAGCAAAA
CTCATGGCTGATGTGGTAGAAGAGGAGACACGGCGATCCCAGCAAGCTGCTCGGGACAAA
CAGAGTCCCAGCCAGGCCAATGGCTGCAGCGACCACAGGCCCATCGACATCCTGGAGATG
CTGAGCAGAGCCAAGGATGAGTATGAGAGGAATCAGATGGGTGACTCAAATATCTCCAGC
CCTGGGTTACAGCCAAGCACTCAGCTCTCCAATCTGGGAAGCACCGAGACTCTAGAAGAA
ATGCCCTCCGGGTCACAGGATAAGTCTGCTCCATCTGGACACAAGCATCTGACGGTAGAA
GAGTTATTTGGAACCTCTTTGCCAAAGGAACAACCAGCAGTTGTGGGTCTGGATTCAGAA
GAAATGGAGAGGTTGCCAGGAGATGCCTCCCAGAAAGAGCCCAATTCATTCCTACCATTT
CCCTTTGAGCAGTTAGGAGGAGCCCCTCAATCAGAAACCCTGGGTGTCCCTTCTGCTGCC
CACCATTCAGTCCAGCCTGAAATCACCACCCCGGTGCTAATCACTCCAGCCTCCATCACA
CAGTCCAATGAAAAGCATGCTCCAACCTACACAATCCCGTTGAGCCCTGTTCTCAGTCCC
ACTCTGCCAGCTGAAGCTCCTACTGCACAGGTTCCCCCCAGCTTACCTCGAAACAGCACC
ATGATGCAGGCAGTGAAGACCACGCCTAGACAGAGGTCTCCACTCCTGAACCAGCCAGTC
CCTGAGCTAAGCCATGCCAGTCTGATTGCCAACCAGAGCCCCTTCAGGGCCCCATTGAAC
GTGACGAACACAGCTGGCACATCCCTCCCAAGCGTTGATCTTCTCCAGAAACTCAGGTTG
ACCCCACAGCATGACCAAATACAGACACAACCACTTGGGAAAGGTGCAATGGTAGCCAGC
TTTTCTCCGGCAGCTGGTCAGCTAGCCACACCTGAGAGCTTCATAGAGCCTCCCTCTAAG
ACAGCAGCAGCAAGAGTGGCGGCCTCAGCCTCCCTGAGCAACATGGTGCTTGCTCCCCTT
CAGTCTATGCAGCAGAACCAGGATCCTGAAGTATTTGTGCAGCCTAAGGTGTTATCCAGT
GCCATCCCGGTTGCAGGCGCCCCACTGGTTACTGCAACGACCACTGCAGTGTCTTCAGTC
CTGCTGGCCCCAAGTGTTTTCCAGCAGACAGTTACAAGATCTTCGGACCTTGAGAGGAAA
GCCAGCTCCCCTTCTCCTCTAACTATTGGAACGCCAGAAAGTCAGAGAAAGCCTTCCATT
ATTCTCAGCAAGTCTCAGCTCCAGGATACATTAATACATCTAATAAAGAATGATTCCAGC
TTCCTCAGTACACTTCATGAAGTCTACTTGCAGGTTCTGACCAAGAACAAAGACAACCAC
AACCTATTG


Coding Sequence Details
Insert Sequence Verified?: N Verification Method: Insert was fully sequenced in parent vector
Reference Sequence Annotations:
End on reference sequence 1759
Start on reference sequence 11
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
5p GTACAAAAAAGCAGGCTCCACC(atg-goi)

3' Linker Sequence:
5p (goi-stop)GACCCAGCTTTCTTGTAC

 

 Recommended Growth Condition:

Distributed in bacterial strain : DH5-alpha T1 phage resistant
Antibiotic Selection: Host Type:  bacterial    Marker:  ampicillin
Host Type:  mammalian    Marker:  blasticidin
Bacterial Selection Condition:  100 ug/mL ampicillin
Growth Condition:  Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:  Commonly used conditions for ampicillin resistant plasmid clones.
Protein Expression Results: None
Recommended expression in: Not Applicable
       DyNA Vector Map

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Vector Name: pLenti6.2/V5-DEST

pLenti6.2V5-DEST in advanced viewed

Synonyms: pLenti6.2/V5-DEST
Sequencing Primer: Forward:  CMV forward
Reverse:  V5 reverse
Description: Lentiviral mammalian expression vector;CMV promoter and C-terminal V5 tag; ampicillin resistance in bacteria; blasticidin resistance in mammalian cells; Gateway recombinational cloning.
Comments: None
Size (bp): 8813
Parent Vector: None
Empty Vector: None
Properties: Gateway, acceptor (destination), lentiviral infection, mammalian expression, recombinational cloning, with tag/fusion/marker
Author Name: Invitrogen
Publications: None
Vector Map:         Vector Sequence:
Sequence:

 



Vector Features:

Type Name Description Start Position End Position
bacterial origin pUC ori pUC origin of replication 7733 8406
death cassette ccdB ccdB death cassette (removed when insert is present) 3767 3976
packaging signal HIV-1 psi HIV-1 psi packaging signal 521 565
poly-A signal SV40 SV40 polyadenylation signal 5639 5770
promoter RSV RSV/5' LTR hybrid promoter 1 410
promoter CMV CMV promoter driving gene of interest 1809 2392
promoter PGK PGK promoter to drive selection marker in mammalian cells 4266 4771
recombination site attR2 attR2 recombinational cloning site 4127 4142
recombination site attR1 attR1 recombinational cloning site 2440 2456
response element HIV-1 RRE HIV-1 Rev response element (RRE) 1075 1308
selectable marker AmpR Ampicillin resistance gene for selection in bacteria 6728 7588
selectable marker BlastR Blasticidin resistance gene in mammalian cells 4849 5249
selectable marker CmR chloramphenicol resistance marker (removed in plasmids with insert) 2673 3329
splice acceptor site 3' splice acceptor two 3' splice acceptors at 1656 and 1684 1656 1656
splice donor site 5' splice donor 5' splice donor 520 520
tag V5 C-terminal V5 epitope tag 4197 4238

 

  • Gene
  • Pathways
  • Protein
  • Clone
  • Organism
  • Genome
  • Reagents

Gene

 

Gene Symbol : DCP1A
Symbol Nomenclature : DCP1A
SYNONYM : HSA275986; Nbla00360; SMAD4IP1; SMIF
Designation : DCP1 decapping enzyme homolog A|Smad4-interacting transcriptional co-activator|decapping enzyme hDcp1a|mRNA-decapping enzyme 1A|putative protein product of Nbla00360|transcription factor SMIF
Full Nomenclature : decapping mRNA 1A
GENEID : 55802
HGNC : 18714
MIM : 607010
Target GenBank: BC007439

 

Pathways

NCI : Regulation of nuclear SMAD2/3 signaling
Reactome : Butyrate Response Factor 1 (BRF1) destabilizes mRNA
Reactome : Deadenylation-dependent mRNA decay
Reactome : Gene Expression
Reactome : Metabolism of RNA
Reactome : Metabolism of mRNA
Reactome : Regulation of mRNA stability by proteins that bind AU-rich elements
Reactome : Tristetraprolin (TTP) destabilizes mRNA
Reactome : mRNA decay by 5' to 3' exoribonuclease

Protein

UniProt : Q9NPI6
HPRD : 06113

Clone

Cloning Information : 269112
HIP Master Clone ID : 170412
Original Clone ID : FLH269112.01L

Organism


TAX_ID : 9606
Species Specific ID: 55802

Genome

Chromosome : 3
Map Location : 3p21.1
Ensembl : ENSG00000162290

Reagents

Labome : DCP1A-antibody