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MTERF (Homo sapiens) in pLenti6.2/V5-DEST (V5-tagged lentiviral mammalian expression vector)

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Explanation of Terms


Gene: Gene Symbol:  MTERF
Gene Name:  mitochondrial transcription termination factor
Sequence             
Original Clone ID: FLH269114.01L
Keyword: None
Species: Homo sapiens
Type: cDNA
Vector Name: pLenti6.2/V5-DEST               Format:  FUSION
Source: HIP
Description: None
Comments: None
Mutation/
Discrepancy :
No / No
Publications: None
Authors:
HsCD00329612

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AVAILABLE
No restriction
Special MTA: None

 

Insert sequence: 1200nts         Open reading frame : 1 to 1200
Reference Sequence Alignment

ATGCAGAGCCTTTCCTTAGGACAAACAAGCATTTCAAAAGGTTTGAACTACCTAACCATT
ATGGCACCAGGAAACCTCTGGCATATGAGAAATAACTTTCTCTTTGGTTCAAGATGTTGG
ATGACTCGATTTTCAGCAGAAAACATCTTCAAATCAGTTTCATTTAGGCTTTTTGGTGTG
AAGTGTCATAATACAGACAGTGAGCCTTTGAAAAATGAGGACCTACTGAAAAACTTACTT
ACTATGGGAGTAGATATTGATATGGCAAGGAAACGACAGCCTGGAGTTTTTCATAGGATG
ATTACCAATGAGCAGGACCTGAAGATGTTCCTTCTTTCCAAAGGAGCTAGCAAAGAAGTG
ATCGCTAGCATCATATCAAGATATCCACGAGCAATAACACGTACTCCCGAGAATCTTTCA
AAACGGTGGGATCTGTGGAGAAAGATTGTGACATCAGACCTTGAAATTGTAAATATTTTG
GAACGTTCTCCTGAATCCTTTTTTCGGTCCAATAACAACCTAAACTTAGAGAATAATATA
AAGTTCCTCTACTCAGTTGGATTGACCCGTAAATGCCTTTGTCGATTGTTGACCAATGCC
CCTCGTACCTTCTCCAATAGTCTTGATCTGAATAAACAGATGGTTGAATTTTTGCAGGCA
GCCGGTTTGTCATTGGGTCACAATGATCCCACAGATTTTGTCAGAAAGATAATTTTTAAA
AACCCTTTTATCTTAATTCAGAGCACCAAGCGGGTGAAAGCTAACATTGAATTCTTACGG
TCAACTTTCAATTTGAACAGTGAGGAACTGCTGGTTCTGATATGTGGTCCAGGAGCTGAA
ATCCTAGACCTTTCCAATGACTATGCCAGAAGAAGCTACGCAAACATCAAAGAGAAGCTG
TTTTCTCTTGGATGTACTGAAGAAGAGGTACAGAAGTTTGTCTTAAGCTATCCAGATGTG
ATCTTCTTGGCAGAGAAAAAGTTTAATGATAAAATAGACTGCCTCATGGAAGAAAACATT
AGCATTTCACAAATAATCGAAAATCCTCGGGTTCTGGATTCAAGCATAAGTACTTTAAAA
AGTCGAATCAAAGAATTGGTAAATGCTGGCTGTAACTTGAGTACTTTAAACATCACTCTT
CTATCTTGGAGTAAAAAAAGATATGAAGCTAAATTGAAAAAGTTAAGCAGATTTGCCTTG


Coding Sequence Details
Insert Sequence Verified?: N Verification Method: Insert was fully sequenced in parent vector
Reference Sequence Annotations:
End on reference sequence 1251
Start on reference sequence 52
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
5p GTACAAAAAAGCAGGCTCCACC(atg-goi)

3' Linker Sequence:
5p (goi-stop)GACCCAGCTTTCTTGTAC

 

 Recommended Growth Condition:

Distributed in bacterial strain : DH5-alpha T1 phage resistant
Antibiotic Selection: Host Type:  bacterial    Marker:  ampicillin
Host Type:  mammalian    Marker:  blasticidin
Bacterial Selection Condition:  100 ug/mL ampicillin
Growth Condition:  Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:  Commonly used conditions for ampicillin resistant plasmid clones.
Protein Expression Results: None
Recommended expression in: Not Applicable
       DyNA Vector Map

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Vector Name: pLenti6.2/V5-DEST

pLenti6.2V5-DEST in advanced viewed

Synonyms: pLenti6.2/V5-DEST
Sequencing Primer: Forward:  CMV forward
Reverse:  V5 reverse
Description: Lentiviral mammalian expression vector;CMV promoter and C-terminal V5 tag; ampicillin resistance in bacteria; blasticidin resistance in mammalian cells; Gateway recombinational cloning.
Comments: None
Size (bp): 8813
Parent Vector: None
Empty Vector: None
Properties: Gateway, acceptor (destination), lentiviral infection, mammalian expression, recombinational cloning, with tag/fusion/marker
Author Name: Invitrogen
Publications: None
Vector Map:         Vector Sequence:
Sequence:

 



Vector Features:

Type Name Description Start Position End Position
bacterial origin pUC ori pUC origin of replication 7733 8406
death cassette ccdB ccdB death cassette (removed when insert is present) 3767 3976
packaging signal HIV-1 psi HIV-1 psi packaging signal 521 565
poly-A signal SV40 SV40 polyadenylation signal 5639 5770
promoter RSV RSV/5' LTR hybrid promoter 1 410
promoter CMV CMV promoter driving gene of interest 1809 2392
promoter PGK PGK promoter to drive selection marker in mammalian cells 4266 4771
recombination site attR2 attR2 recombinational cloning site 4127 4142
recombination site attR1 attR1 recombinational cloning site 2440 2456
response element HIV-1 RRE HIV-1 Rev response element (RRE) 1075 1308
selectable marker AmpR Ampicillin resistance gene for selection in bacteria 6728 7588
selectable marker BlastR Blasticidin resistance gene in mammalian cells 4849 5249
selectable marker CmR chloramphenicol resistance marker (removed in plasmids with insert) 2673 3329
splice acceptor site 3' splice acceptor two 3' splice acceptors at 1656 and 1684 1656 1656
splice donor site 5' splice donor 5' splice donor 520 520
tag V5 C-terminal V5 epitope tag 4197 4238

 

  • Gene
  • Pathways
  • Protein
  • Clone
  • Organism
  • Genome
  • Reagents

Gene

 

Gene Symbol : MTERF
Symbol Nomenclature : MTERF
Designation : mitochondrial transcription termination factor 1|transcription termination factor, mitochondrial
Full Nomenclature : mitochondrial transcription termination factor
GENEID : 7978
HGNC : 21463
MIM : 602318
Vega : OTTHUMG00000156057
Target GenBank: BC000965

 

Reference Sequence Alignment

  

HsCD00329612        ATGCAGAGCCTTTCCTTAGGACAAACAAGCATTTCAAAAGGTTTGAACTACCTAACCATT
XM_005250594.1      ------------------------------------------------------------
XM_005250595.1      ------------------------------------------------------------
NM_006980.3         ATGCAGAGCCTTTCCTTAGGACAAACAAGCATTTCAAAAGGTTTGAACTACCTAACCATT
XM_005250593.1      ATGCAGAGCCTTTCCTTAGGACAAACAAGCATTTCAAAAGGTTTGAACTACCTAACCATT
                                                                                

HsCD00329612        ATGGCACCAGGAAACCTCTGGCATATGAGAAATAACTTTCTCTTTGGTTCAAGATGTTGG
XM_005250594.1      ATGGCACCAGGAAACCTCTGGCATATGAGAAATAACTTTCTCTTTGGTTCAAGATGTTGG
XM_005250595.1      ATGGCACCAGGAAACCTCTGGCATATGAGAAATAACTTTCTCTTTGGTTCAAGATGTTGG
NM_006980.3         ATGGCACCAGGAAACCTCTGGCATATGAGAAATAACTTTCTCTTTGGTTCAAGATGTTGG
XM_005250593.1      ATGGCACCAGGAAACCTCTGGCATATGAGAAATAACTTTCTCTTTGGTTCAAGATGTTGG
                    ************************************************************

HsCD00329612        ATGACTCGATTTTCAGCAGAAAACATCTTCAAATCAGTTTCATTTAGGCTTTTTGGTGTG
XM_005250594.1      ATGACTCGATTTTCAGCAGAAAACATCTTCAAATCAGTTTCATTTAGGCTTTTTGGTGTG
XM_005250595.1      ATGACTCGATTTTCAGCAGAAAACATCTTCAAATCAGTTTCATTTAGGCTTTTTGGTGTG
NM_006980.3         ATGACTCGATTTTCAGCAGAAAACATCTTCAAATCAGTTTCATTTAGGCTTTTTGGTGTG
XM_005250593.1      ATGACTCGATTTTCAGCAGAAAACATCTTCAAATCAGTTTCATTTAGGCTTTTTGGTGTG
                    ************************************************************

HsCD00329612        AAGTGTCATAATACAGACAGTGAGCCTTTGAAAAATGAGGACCTACTGAAAAACTTACTT
XM_005250594.1      AAGTGTCATAATACAGACAGTGAGCCTTTGAAAAATGAGGACCTACTGAAAAACTTACTT
XM_005250595.1      AAGTGTCATAATACAGACAGTGAGCCTTTGAAAAATGAGGACCTACTGAAAAACTTACTT
NM_006980.3         AAGTGTCATAATACAGACAGTGAGCCTTTGAAAAATGAGGACCTACTGAAAAACTTACTT
XM_005250593.1      AAGTGTCATAATACAGACAGTGAGCCTTTGAAAAATGAGGACCTACTGAAAAACTTACTT
                    ************************************************************

HsCD00329612        ACTATGGGAGTAGATATTGATATGGCAAGGAAACGACAGCCTGGAGTTTTTCATAGGATG
XM_005250594.1      ACTATGGGAGTAGATATTGACATGGCAAGGAAACGACAGCCTGGAGTTTTTCATAGGATG
XM_005250595.1      ACTATGGGAGTAGATATTGACATGGCAAGGAAACGACAGCCTGGAGTTTTTCATAGGATG
NM_006980.3         ACTATGGGAGTAGATATTGACATGGCAAGGAAACGACAGCCTGGAGTTTTTCATAGGATG
XM_005250593.1      ACTATGGGAGTAGATATTGACATGGCAAGGAAACGACAGCCTGGAGTTTTTCATAGGATG
                    ******************** ***************************************

HsCD00329612        ATTACCAATGAGCAGGACCTGAAGATGTTCCTTCTTTCCAAAGGAGCTAGCAAAGAAGTG
XM_005250594.1      ATTACCAATGAGCAGGACCTGAAGATGTTCCTTCTTTCCAAAGGAGCTAGCAAAGAAGTG
XM_005250595.1      ATTACCAATGAGCAGGACCTGAAGATGTTCCTTCTTTCCAAAGGAGCTAGCAAAGAAGTG
NM_006980.3         ATTACCAATGAGCAGGACCTGAAGATGTTCCTTCTTTCCAAAGGAGCTAGCAAAGAAGTG
XM_005250593.1      ATTACCAATGAGCAGGACCTGAAGATGTTCCTTCTTTCCAAAGGAGCTAGCAAAGAAGTG
                    ************************************************************

HsCD00329612        ATCGCTAGCATCATATCAAGATATCCACGAGCAATAACACGTACTCCCGAGAATCTTTCA
XM_005250594.1      ATCGCTAGCATCATATCAAGATATCCACGAGCAATAACACGTACTCCCGAGAATCTTTCA
XM_005250595.1      ATCGCTAGCATCATATCAAGATATCCACGAGCAATAACACGTACTCCCGAGAATCTTTCA
NM_006980.3         ATCGCTAGCATCATATCAAGATATCCACGAGCAATAACACGTACTCCCGAGAATCTTTCA
XM_005250593.1      ATCGCTAGCATCATATCAAGATATCCACGAGCAATAACACGTACTCCCGAGAATCTTTCA
                    ************************************************************

HsCD00329612        AAACGGTGGGATCTGTGGAGAAAGATTGTGACATCAGACCTTGAAATTGTAAATATTTTG
XM_005250594.1      AAACGGTGGGATCTGTGGAGAAAGATTGTGACATCAGACCTTGAAATTGTAAATATTTTG
XM_005250595.1      AAACGGTGGGATCTGTGGAGAAAGATTGTGACATCAGACCTTGAAATTGTAAATATTTTG
NM_006980.3         AAACGGTGGGATCTGTGGAGAAAGATTGTGACATCAGACCTTGAAATTGTAAATATTTTG
XM_005250593.1      AAACGGTGGGATCTGTGGAGAAAGATTGTGACATCAGACCTTGAAATTGTAAATATTTTG
                    ************************************************************

HsCD00329612        GAACGTTCTCCTGAATCCTTTTTTCGGTCCAATAACAACCTAAACTTAGAGAATAATATA
XM_005250594.1      GAACGTTCTCCTGAATCCTTTTTTCGGTCCAATAACAACCTAAACTTAGAGAATAATATA
XM_005250595.1      GAACGTTCTCCTGAATCCTTTTTTCGGTCCAATAACAACCTAAACTTAGAGAATAATATA
NM_006980.3         GAACGTTCTCCTGAATCCTTTTTTCGGTCCAATAACAACCTAAACTTAGAGAATAATATA
XM_005250593.1      GAACGTTCTCCTGAATCCTTTTTTCGGTCCAATAACAACCTAAACTTAGAGAATAATATA
                    ************************************************************

HsCD00329612        AAGTTCCTCTACTCAGTTGGATTGACCCGTAAATGCCTTTGTCGATTGTTGACCAATGCC
XM_005250594.1      AAGTTCCTCTACTCAGTTGGATTGACCCGTAAATGCCTTTGTCGATTGTTGACCAATGCC
XM_005250595.1      AAGTTCCTCTACTCAGTTGGATTGACCCGTAAATGCCTTTGTCGATTGTTGACCAATGCC
NM_006980.3         AAGTTCCTCTACTCAGTTGGATTGACCCGTAAATGCCTTTGTCGATTGTTGACCAATGCC
XM_005250593.1      AAGTTCCTCTACTCAGTTGGATTGACCCGTAAATGCCTTTGTCGATTGTTGACCAATGCC
                    ************************************************************

HsCD00329612        CCTCGTACCTTCTCCAATAGTCTTGATCTGAATAAACAGATGGTTGAATTTTTGCAGGCA
XM_005250594.1      CCTCGTACCTTCTCCAATAGTCTTGATCTGAATAAACAGATGGTTGAATTTTTGCAGGCA
XM_005250595.1      CCTCGTACCTTCTCCAATAGTCTTGATCTGAATAAACAGATGGTTGAATTTTTGCAGGCA
NM_006980.3         CCTCGTACCTTCTCCAATAGTCTTGATCTGAATAAACAGATGGTTGAATTTTTGCAGGCA
XM_005250593.1      CCTCGTACCTTCTCCAATAGTCTTGATCTGAATAAACAGATGGTTGAATTTTTGCAGGCA
                    ************************************************************

HsCD00329612        GCCGGTTTGTCATTGGGTCACAATGATCCCACAGATTTTGTCAGAAAGATAATTTTTAAA
XM_005250594.1      GCCGGTTTGTCATTGGGTCACAATGATCCCGCAGATTTTGTCAGAAAGATAATTTTTAAA
XM_005250595.1      GCCGGTTTGTCATTGGGTCACAATGATCCCGCAGATTTTGTCAGAAAGATAATTTTTAAA
NM_006980.3         GCCGGTTTGTCATTGGGTCACAATGATCCCGCAGATTTTGTCAGAAAGATAATTTTTAAA
XM_005250593.1      GCCGGTTTGTCATTGGGTCACAATGATCCCGCAGATTTTGTCAGAAAGATAATTTTTAAA
                    ******************************.*****************************

HsCD00329612        AACCCTTTTATCTTAATTCAGAGCACCAAGCGGGTGAAAGCTAACATTGAATTCTTACGG
XM_005250594.1      AACCCTTTTATCTTAATTCAGAGCACCAAGCGGGTGAAAGCTAACATTGAATTCTTACGG
XM_005250595.1      AACCCTTTTATCTTAATTCAGAGCACCAAGCGGGTGAAAGCTAACATTGAATTCTTACGG
NM_006980.3         AACCCTTTTATCTTAATTCAGAGCACCAAGCGGGTGAAAGCTAACATTGAATTCTTACGG
XM_005250593.1      AACCCTTTTATCTTAATTCAGAGCACCAAGCGGGTGAAAGCTAACATTGAATTCTTACGG
                    ************************************************************

HsCD00329612        TCAACTTTCAATTTGAACAGTGAGGAACTGCTGGTTCTGATATGTGGTCCAGGAGCTGAA
XM_005250594.1      TCAACTTTCAATTTGAACAGTGAGGAACTGCTGGTTCTGATATGTGGTCCAGGAGCTGAA
XM_005250595.1      TCAACTTTCAATTTGAACAGTGAGGAACTGCTGGTTCTGATATGTGGTCCAGGAGCTGAA
NM_006980.3         TCAACTTTCAATTTGAACAGTGAGGAACTGCTGGTTCTGATATGTGGTCCAGGAGCTGAA
XM_005250593.1      TCAACTTTCAATTTGAACAGTGAGGAACTGCTGGTTCTGATATGTGGTCCAGGAGCTGAA
                    ************************************************************

HsCD00329612        ATCCTAGACCTTTCCAATGACTATGCCAGAAGAAGCTACGCAAACATCAAAGAGAAGCTG
XM_005250594.1      ATCCTAGACCTTTCCAATGACTATGCCAGAAGAAGCTACGCAAACATCAAAGAGAAGCTG
XM_005250595.1      ATCCTAGACCTTTCCAATGACTATGCCAGAAGAAGCTACGCAAACATCAAAGAGAAGCTG
NM_006980.3         ATCCTAGACCTTTCCAATGACTATGCCAGAAGAAGCTACGCAAACATCAAAGAGAAGCTG
XM_005250593.1      ATCCTAGACCTTTCCAATGACTATGCCAGAAGAAGCTACGCAAACATCAAAGAGAAGCTG
                    ************************************************************

HsCD00329612        TTTTCTCTTGGATGTACTGAAGAAGAGGTACAGAAGTTTGTCTTAAGCTATCCAGATGTG
XM_005250594.1      TTTTCTCTTGGATGTACTGAAGAAGAGGTACAGAAGTTTGTCTTAAGCTATCCAGATGTG
XM_005250595.1      TTTTCTCTTGGATGTACTGAAGAAGAGGTACAGAAGTTTGTCTTAAGCTATCCAGATGTG
NM_006980.3         TTTTCTCTTGGATGTACTGAAGAAGAGGTACAGAAGTTTGTCTTAAGCTATCCAGATGTG
XM_005250593.1      TTTTCTCTTGGATGTACTGAAGAAGAGGTACAGAAGTTTGTCTTAAGCTATCCAGATGTG
                    ************************************************************

HsCD00329612        ATCTTCTTGGCAGAGAAAAAGTTTAATGATAAAATAGACTGCCTCATGGAAGAAAACATT
XM_005250594.1      ATCTTCTTGGCAGAGAAAAAGTTTAATGATAAAATAGACTGCCTCATGGAAGAAAACATT
XM_005250595.1      ATCTTCTTGGCAGAGAAAAAGTTTAATGATAAAATAGACTGCCTCATGGAAGAAAACATT
NM_006980.3         ATCTTCTTGGCAGAGAAAAAGTTTAATGATAAAATAGACTGCCTCATGGAAGAAAACATT
XM_005250593.1      ATCTTCTTGGCAGAGAAAAAGTTTAATGATAAAATAGACTGCCTCATGGAAGAAAACATT
                    ************************************************************

HsCD00329612        AGCATTTCACAAATAATCGAAAATCCTCGGGTTCTGGATTCAAGCATAAGTACTTTAAAA
XM_005250594.1      AGCATTTCACAAATAATCGAAAATCCTCGGGTTCTGGATTCAAGCATAAGTACTTTAAAA
XM_005250595.1      AGCATTTCACAAATAATCGAAAATCCTCGGGTTCTGGATTCAAGCATAAGTACTTTAAAA
NM_006980.3         AGCATTTCACAAATAATCGAAAATCCTCGGGTTCTGGATTCAAGCATAAGTACTTTAAAA
XM_005250593.1      AGCATTTCACAAATAATCGAAAATCCTCGGGTTCTGGATTCAAGCATAAGTACTTTAAAA
                    ************************************************************

HsCD00329612        AGTCGAATCAAAGAATTGGTAAATGCTGGCTGTAACTTGAGTACTTTAAACATCACTCTT
XM_005250594.1      AGTCGAATCAAAGAATTGGTAAATGCTGGCTGTAACTTGAGTACTTTAAACATCACTCTT
XM_005250595.1      AGTCGAATCAAAGAATTGGTAAATGCTGGCTGTAACTTGAGTACTTTAAACATCACTCTT
NM_006980.3         AGTCGAATCAAAGAATTGGTAAATGCTGGCTGTAACTTGAGTACTTTAAACATCACTCTT
XM_005250593.1      AGTCGAATCAAAGAATTGGTAAATGCTGGCTGTAACTTGAGTACTTTAAACATCACTCTT
                    ************************************************************

HsCD00329612        CTATCTTGGAGTAAAAAAAGATATGAAGCTAAATTGAAAAAGTTAAGCAGATTTGCCTTG
XM_005250594.1      CTATCTTGGAGTAAAAAAAGATATGAAGCTAAATTGAAAAAGTTAAGCAGATTTGCCTAA
XM_005250595.1      CTATCTTGGAGTAAAAAAAGATATGAAGCTAAATTGAAAAAGTTAAGCAGATTTGCCTAA
NM_006980.3         CTATCTTGGAGTAAAAAAAGATATGAAGCTAAATTGAAAAAGTTAAGCAGATTTGCCTAA
XM_005250593.1      CTATCTTGGAGTAAAAAAAGATATGAAGCTAAATTGAAAAAGTTAAGCAGATTTGCCTAA
                    **********************************************************:.

Pathways

Reactome : Gene Expression
Reactome : Mitochondrial biogenesis
Reactome : Mitochondrial transcription termination
Reactome : Organelle biogenesis and maintenance
Reactome : RNA Polymerase I, RNA Polymerase III, and Mitochondrial Transcription
Reactome : Transcription
Reactome : Transcription from mitochondrial promoters
Reactome : Transcriptional activation of mitochondrial biogenesis
WikiPathway : Mitochondrial Gene Expression

Protein

SMART domain : Mterf : Mitochondrial termination factor repeats
UniProt : Q99551
HPRD : 08373

Clone

Cloning Information : 269114
HIP Master Clone ID : 141553
Original Clone ID : FLH269114.01L

Organism


TAX_ID : 9606
Species Specific ID: 7978

Genome

Chromosome : 7
Map Location : 7q21-q22
Ensembl : ENSG00000127989