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ARNT2 (Homo sapiens) in pLenti6.2/V5-DEST (V5-tagged lentiviral mammalian expression vector)

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Explanation of Terms


Gene: Gene Symbol:  ARNT2
Gene Name:  aryl-hydrocarbon receptor nuclear translocator 2
Sequence             
Original Clone ID: FLH269115.01L
Keyword: None
Species: Homo sapiens
Type: cDNA
Vector Name: pLenti6.2/V5-DEST               Format:  FUSION
Source: HIP
Description: None
Comments: None
Mutation/
Discrepancy :
No / No
Publications: None
Authors:
HsCD00329613

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AVAILABLE
No restriction
Special MTA: None

 

Insert sequence: 2151nts         Open reading frame : 1 to 2151
Reference Sequence Alignment

ATGGCAACCCCGGCGGCGGTCAACCCTCCGGAAATGGCTTCAGACATACCTGGATCTGTG
ACGTTGCCCGTTGCCCCCATGGCGGCCACCGGACAGGTGAGGATGGCGGGGGCCATGCCT
GCCCGTGGAGGAAAGCGGCGTTCCGGAATGGACTTCGATGATGAAGATGGTGAAGGCCCC
AGTAAATTTTCAAGAGAGAATCATAGTGAAATCGAAAGGCGCAGACGGAACAAGATGACT
CAGTACATCACGGAGCTCTCCGACATGGTCCCCACATGCAGCGCACTGGCTCGGAAGCCA
GACAAGCTCACCATCCTCCGCATGGCCGTCTCGCACATGAAGTCCATGAGGGGTACAGGG
AACAAGTCCACCGATGGCGCGTACAAGCCTTCCTTCCTCACAGAGCAGGAACTGAAGCAT
CTCATCCTTGAAGCAGCTGATGGATTTCTGTTTGTGGTGGCTGCTGAGACAGGGCGAGTG
ATTTATGTGTCTGACTCCGTCACCCCTGTTCTGAACCAGCCCCAGTCAGAGTGGTTTGGG
AGCACACTGTATGAACAGGTGCATCCTGATGACGTGGAGAAGCTGAGAGAGCAACTGTGC
ACCTCAGAAAACTCAATGACAGGCCGGATCTTGGACCTGAAGACTGGGACGGTCAAGAAA
GAAGGGCAGCAGTCATCCATGAGGATGTGCATGGGCTCGCGGCGGTCTTTCATCTGCAGG
ATGAGGTGTGGAAATGCTCCTTTGGACCACCTTCCTCTAAACAGAATAACCACCATGAGG
AAAAGGTTCAGGAATGGCCTTGGCCCTGTGAAAGAAGGAGAAGCCCAATATGCTGTGGTC
CACTGTACAGGATACATCAAGGCCTGGCCACCAGCAGGAATGACCATACCTGAAGAAGAC
GCTGATGTGGGACAAGGCAGTAAATATTGCCTCGTGGCAATTGGGAGACTCCAGGTGACC
AGCTCTCCTGTATGCATGGACATGAATGGGATGTCGGTGCCCACAGAGTTCTTATCCCGG
CATAACTCCGATGGAATCATCACATTTGTGGATCCAAGATGTATCAGTGTGATTGGCTAC
CAACCGCAGGATCTTCTGGGAAAGGACATTTTGGAATTCTGCCACCCTGAGGATCAAAGC
CATCTGCGTGAGAGCTTCCAGCAGGTGGTTAAGCTGAAAGGCCAAGTCCTGTCGGTCATG
TATCGATTTCGCACCAAGAACCGGGAGTGGATGTTGATCCGCACCAGCAGCTTCACATTC
CAGAATCCCTATTCTGATGAGATTGAGTACATCATCTGCACCAACACCAACGTCAAGCAA
CTTCAGCAACAGCAGGCAGAATTGGAAGTGCACCAGAGAGATGGATTGTCATCGTATGAC
TTATCCCAGGTCCCCGTCCCCAACCTACCAGCCGGTGTTCATGAGGCCGGGAAGTCCGTG
GAAAAGGCGGATGCAATCTTCTCCCAGGAAAGAGATCCTCGGTTTGCTGAAATGTTTGCA
GGAATTAGTGCATCGGAGAAGAAGATGATGAGCTCAGCCTCTGCAGCAGGAACCCAGCAG
ATCTACTCCCAAGGAAGCCCATTTCCCTCTGGACACTCCGGGAAGGCCTTCAGCTCTTCA
GTGGTTCATGTGCCTGGAGTGAATGATATTCAGTCCTCTTCTTCCACGGGCCAGAACATG
TCCCAAATCTCCCGGCAGCTAAACCAGAGTCAGGTGGCATGGACAGGGAGTCGTCCGCCC
TTTCCGGGACAGCAAATCCCATCTCAGTCCAGCAAGACTCAGTCATCTCCCTTTGGGATT
GGAACGAGCCACACCTACCCGGCAGACCCCTCTTCCTACAGCCCCCTCTCCAGCCCAGCT
ACCTCCTCGCCAAGTGGGAATGCCTACTCCAGTCTTGCCAACAGGACTCCAGGGTTCGCT
GAAAGTGGACAAAGTAGCGGGCAGTTCCAAGGGCGGCCCTCGGAAGTCTGGTCGCAGTGG
CAAAGCCAGCACCATGGCCAGCAGAGCGGTGAGCAGCACTCCCACCAGCAGCCCGGTCAG
ACTGAAGTGTTCCAGGACATGCTGCCCATGCCAGGAGATCCAGGGAATGAATGGTGGTCT
CCCCACTCCCGGCAGCACTTTAGGCAGCCCATAAGCTATGCGAGAATGTTG


Coding Sequence Details
Insert Sequence Verified?: N Verification Method: Insert was fully sequenced in parent vector
Reference Sequence Annotations:
End on reference sequence 2266
Start on reference sequence 116
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
5p GTACAAAAAAGCAGGCTCCACC(atg-goi)

3' Linker Sequence:
5p (goi-stop)GACCCAGCTTTCTTGTAC

 

 Recommended Growth Condition:

Distributed in bacterial strain : DH5-alpha T1 phage resistant
Antibiotic Selection: Host Type:  bacterial    Marker:  ampicillin
Host Type:  mammalian    Marker:  blasticidin
Bacterial Selection Condition:  100 ug/mL ampicillin
Growth Condition:  Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:  Commonly used conditions for ampicillin resistant plasmid clones.
Protein Expression Results: None
Recommended expression in: Not Applicable
       DyNA Vector Map

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Vector Name: pLenti6.2/V5-DEST

pLenti6.2V5-DEST in advanced viewed

Synonyms: pLenti6.2/V5-DEST
Sequencing Primer: Forward:  CMV forward
Reverse:  V5 reverse
Description: Lentiviral mammalian expression vector;CMV promoter and C-terminal V5 tag; ampicillin resistance in bacteria; blasticidin resistance in mammalian cells; Gateway recombinational cloning.
Comments: None
Size (bp): 8813
Parent Vector: None
Empty Vector: None
Properties: Gateway, acceptor (destination), lentiviral infection, mammalian expression, recombinational cloning, with tag/fusion/marker
Author Name: Invitrogen
Publications: None
Vector Map:         Vector Sequence:
Sequence:

 



Vector Features:

Type Name Description Start Position End Position
bacterial origin pUC ori pUC origin of replication 7733 8406
death cassette ccdB ccdB death cassette (removed when insert is present) 3767 3976
packaging signal HIV-1 psi HIV-1 psi packaging signal 521 565
poly-A signal SV40 SV40 polyadenylation signal 5639 5770
promoter RSV RSV/5' LTR hybrid promoter 1 410
promoter CMV CMV promoter driving gene of interest 1809 2392
promoter PGK PGK promoter to drive selection marker in mammalian cells 4266 4771
recombination site attR2 attR2 recombinational cloning site 4127 4142
recombination site attR1 attR1 recombinational cloning site 2440 2456
response element HIV-1 RRE HIV-1 Rev response element (RRE) 1075 1308
selectable marker AmpR Ampicillin resistance gene for selection in bacteria 6728 7588
selectable marker BlastR Blasticidin resistance gene in mammalian cells 4849 5249
selectable marker CmR chloramphenicol resistance marker (removed in plasmids with insert) 2673 3329
splice acceptor site 3' splice acceptor two 3' splice acceptors at 1656 and 1684 1656 1656
splice donor site 5' splice donor 5' splice donor 520 520
tag V5 C-terminal V5 epitope tag 4197 4238

 

  • Gene
  • Protein
  • Clone
  • Organism
  • Genome
  • Reagents

Gene

 

Gene Symbol : ARNT2
Symbol Nomenclature : ARNT2
SYNONYM : bHLHe1
Designation : ARNT protein 2|aryl hydrocarbon receptor nuclear translocator 2|class E basic helix-loop-helix protein 1
Full Nomenclature : aryl-hydrocarbon receptor nuclear translocator 2
GENEID : 9915
HGNC : 16876
MIM : 606036
Vega : OTTHUMG00000165478
Target GenBank: BC036099

 

Reference Sequence Alignment

  

HsCD00329613      ATGGCAACCCCGGCGGCGGTCAACCCTCCGGAAATGGCTTCAGACATACCTGGATCTGTG
NM_014862.3       ATGGCAACCCCGGCGGCGGTCAACCCTCCGGAAATGGCTTCAGACATACCTGGATCTGTG
                  ************************************************************

HsCD00329613      ACGTTGCCCGTTGCCCCCATGGCGGCCACCGGACAGGTGAGGATGGCGGGGGCCATGCCT
NM_014862.3       ACGTTGCCCGTTGCCCCCATGGCGGCCACCGGACAGGTGAGGATGGCGGGGGCCATGCCT
                  ************************************************************

HsCD00329613      GCCCGTGGAGGAAAGCGGCGTTCCGGAATGGACTTCGATGATGAAGATGGTGAAGGCCCC
NM_014862.3       GCCCGTGGAGGAAAGCGGCGTTCCGGAATGGACTTCGATGATGAAGATGGTGAAGGCCCC
                  ************************************************************

HsCD00329613      AGTAAATTTTCAAGAGAGAATCATAGTGAAATCGAAAGGCGCAGACGGAACAAGATGACT
NM_014862.3       AGTAAATTTTCAAGAGAGAATCATAGTGAAATCGAAAGGCGCAGACGGAACAAGATGACT
                  ************************************************************

HsCD00329613      CAGTACATCACGGAGCTCTCCGACATGGTCCCCACATGCAGCGCACTGGCTCGGAAGCCA
NM_014862.3       CAGTACATCACGGAGCTCTCCGACATGGTCCCCACATGCAGCGCACTGGCTCGGAAGCCA
                  ************************************************************

HsCD00329613      GACAAGCTCACCATCCTCCGCATGGCCGTCTCGCACATGAAGTCCATGAGGGGTACAGGG
NM_014862.3       GACAAGCTCACCATCCTCCGCATGGCCGTCTCGCACATGAAGTCCATGAGGGGTACAGGG
                  ************************************************************

HsCD00329613      AACAAGTCCACCGATGGCGCGTACAAGCCTTCCTTCCTCACAGAGCAGGAACTGAAGCAT
NM_014862.3       AACAAGTCCACCGATGGCGCGTACAAGCCTTCCTTCCTCACAGAGCAGGAACTGAAGCAT
                  ************************************************************

HsCD00329613      CTCATCCTTGAAGCAGCTGATGGATTTCTGTTTGTGGTGGCTGCTGAGACAGGGCGAGTG
NM_014862.3       CTCATCCTTGAAGCAGCTGATGGATTTCTGTTTGTGGTGGCTGCTGAGACAGGGCGAGTG
                  ************************************************************

HsCD00329613      ATTTATGTGTCTGACTCCGTCACCCCTGTTCTGAACCAGCCCCAGTCAGAGTGGTTTGGG
NM_014862.3       ATTTATGTGTCTGACTCCGTCACCCCTGTTCTGAACCAGCCCCAGTCAGAGTGGTTTGGG
                  ************************************************************

HsCD00329613      AGCACACTGTATGAACAGGTGCATCCTGATGACGTGGAGAAGCTGAGAGAGCAACTGTGC
NM_014862.3       AGCACACTGTATGAACAGGTGCATCCTGATGACGTGGAGAAGCTGAGAGAGCAACTGTGC
                  ************************************************************

HsCD00329613      ACCTCAGAAAACTCAATGACAGGCCGGATCTTGGACCTGAAGACTGGGACGGTCAAGAAA
NM_014862.3       ACCTCAGAAAACTCAATGACAGGCCGGATCTTGGACCTGAAGACTGGGACGGTCAAGAAA
                  ************************************************************

HsCD00329613      GAAGGGCAGCAGTCATCCATGAGGATGTGCATGGGCTCGCGGCGGTCTTTCATCTGCAGG
NM_014862.3       GAAGGGCAGCAGTCATCCATGAGGATGTGCATGGGCTCGCGGCGGTCTTTCATCTGCAGG
                  ************************************************************

HsCD00329613      ATGAGGTGTGGAAATGCTCCTTTGGACCACCTTCCTCTAAACAGAATAACCACCATGAGG
NM_014862.3       ATGAGGTGTGGAAATGCTCCTTTGGACCACCTTCCTCTAAACAGAATAACCACCATGAGG
                  ************************************************************

HsCD00329613      AAAAGGTTCAGGAATGGCCTTGGCCCTGTGAAAGAAGGAGAAGCCCAATATGCTGTGGTC
NM_014862.3       AAAAGGTTCAGGAATGGCCTTGGCCCTGTGAAAGAAGGAGAAGCCCAATATGCTGTGGTC
                  ************************************************************

HsCD00329613      CACTGTACAGGATACATCAAGGCCTGGCCACCAGCAGGAATGACCATACCTGAAGAAGAC
NM_014862.3       CACTGTACAGGATACATCAAGGCCTGGCCACCAGCAGGAATGACCATACCTGAAGAAGAC
                  ************************************************************

HsCD00329613      GCTGATGTGGGACAAGGCAGTAAATATTGCCTCGTGGCAATTGGGAGACTCCAGGTGACC
NM_014862.3       GCTGATGTGGGACAAGGCAGTAAATATTGCCTCGTGGCAATTGGGAGACTCCAGGTGACC
                  ************************************************************

HsCD00329613      AGCTCTCCTGTATGCATGGACATGAATGGGATGTCGGTGCCCACAGAGTTCTTATCCCGG
NM_014862.3       AGCTCTCCTGTATGCATGGACATGAATGGGATGTCGGTGCCCACAGAGTTCTTATCCCGG
                  ************************************************************

HsCD00329613      CATAACTCCGATGGAATCATCACATTTGTGGATCCAAGATGTATCAGTGTGATTGGCTAC
NM_014862.3       CATAACTCCGATGGAATCATCACATTTGTGGATCCAAGATGTATCAGTGTGATTGGCTAC
                  ************************************************************

HsCD00329613      CAACCGCAGGATCTTCTGGGAAAGGACATTTTGGAATTCTGCCACCCTGAGGATCAAAGC
NM_014862.3       CAACCCCAGGATCTTCTGGGAAAGGACATTTTGGAATTCTGCCACCCTGAGGATCAAAGC
                  ***** ******************************************************

HsCD00329613      CATCTGCGTGAGAGCTTCCAGCAGGTGGTTAAGCTGAAAGGCCAAGTCCTGTCGGTCATG
NM_014862.3       CATCTGCGTGAGAGCTTCCAGCAGGTGGTTAAGCTGAAAGGCCAAGTCCTGTCGGTCATG
                  ************************************************************

HsCD00329613      TATCGATTTCGCACCAAGAACCGGGAGTGGATGTTGATCCGCACCAGCAGCTTCACATTC
NM_014862.3       TATCGATTTCGCACCAAGAACCGGGAGTGGATGTTGATCCGCACCAGCAGCTTCACATTC
                  ************************************************************

HsCD00329613      CAGAATCCCTATTCTGATGAGATTGAGTACATCATCTGCACCAACACCAACGTCAAGCAA
NM_014862.3       CAGAATCCCTATTCTGATGAGATTGAGTACATCATCTGCACCAACACCAACGTCAAGCAA
                  ************************************************************

HsCD00329613      CTTCAGCAACAGCAGGCAGAATTGGAAGTGCACCAGAGAGATGGATTGTCATCGTATGAC
NM_014862.3       CTTCAGCAACAGCAGGCAGAATTGGAAGTGCACCAGAGAGATGGATTGTCATCGTATGAC
                  ************************************************************

HsCD00329613      TTATCCCAGGTCCCCGTCCCCAACCTACCAGCCGGTGTTCATGAGGCCGGGAAGTCCGTG
NM_014862.3       TTATCCCAGGTCCCCGTCCCCAACCTACCAGCCGGTGTTCATGAGGCCGGGAAGTCCGTG
                  ************************************************************

HsCD00329613      GAAAAGGCGGATGCAATCTTCTCCCAGGAAAGAGATCCTCGGTTTGCTGAAATGTTTGCA
NM_014862.3       GAAAAGGCGGATGCAATCTTCTCCCAGGAAAGAGATCCTCGGTTTGCTGAAATGTTTGCA
                  ************************************************************

HsCD00329613      GGAATTAGTGCATCGGAGAAGAAGATGATGAGCTCAGCCTCTGCAGCAGGAACCCAGCAG
NM_014862.3       GGAATTAGTGCATCGGAGAAGAAGATGATGAGCTCAGCCTCTGCAGCAGGAACCCAGCAG
                  ************************************************************

HsCD00329613      ATCTACTCCCAAGGAAGCCCATTTCCCTCTGGACACTCCGGGAAGGCCTTCAGCTCTTCA
NM_014862.3       ATCTACTCCCAAGGAAGCCCATTTCCCTCTGGACACTCCGGGAAGGCCTTCAGCTCTTCA
                  ************************************************************

HsCD00329613      GTGGTTCATGTGCCTGGAGTGAATGATATTCAGTCCTCTTCTTCCACGGGCCAGAACATG
NM_014862.3       GTGGTTCATGTGCCTGGAGTGAATGATATTCAGTCCTCTTCTTCCACGGGCCAGAACATG
                  ************************************************************

HsCD00329613      TCCCAAATCTCCCGGCAGCTAAACCAGAGTCAGGTGGCATGGACAGGGAGTCGTCCGCCC
NM_014862.3       TCCCAAATCTCCCGGCAGCTAAACCAGAGTCAGGTGGCATGGACAGGGAGTCGTCCGCCC
                  ************************************************************

HsCD00329613      TTTCCGGGACAGCAAATCCCATCTCAGTCCAGCAAGACTCAGTCATCTCCCTTTGGGATT
NM_014862.3       TTTCCGGGACAGCAAATCCCATCTCAGTCCAGCAAGACTCAGTCATCTCCCTTTGGGATT
                  ************************************************************

HsCD00329613      GGAACGAGCCACACCTACCCGGCAGACCCCTCTTCCTACAGCCCCCTCTCCAGCCCAGCT
NM_014862.3       GGAACGAGCCACACCTACCCGGCAGACCCCTCTTCCTACAGCCCCCTCTCCAGCCCAGCT
                  ************************************************************

HsCD00329613      ACCTCCTCGCCAAGTGGGAATGCCTACTCCAGTCTTGCCAACAGGACTCCAGGGTTCGCT
NM_014862.3       ACCTCCTCGCCAAGTGGGAATGCCTACTCCAGTCTTGCCAACAGGACTCCAGGGTTCGCT
                  ************************************************************

HsCD00329613      GAAAGTGGACAAAGTAGCGGGCAGTTCCAAGGGCGGCCCTCGGAAGTCTGGTCGCAGTGG
NM_014862.3       GAAAGTGGACAAAGTAGCGGGCAGTTCCAAGGGCGGCCCTCGGAAGTCTGGTCGCAGTGG
                  ************************************************************

HsCD00329613      CAAAGCCAGCACCATGGCCAGCAGAGCGGTGAGCAGCACTCCCACCAGCAGCCCGGTCAG
NM_014862.3       CAAAGCCAGCACCATGGCCAGCAGAGCGGTGAGCAGCACTCCCACCAGCAGCCCGGTCAG
                  ************************************************************

HsCD00329613      ACTGAAGTGTTCCAGGACATGCTGCCCATGCCAGGAGATCCAGGGAATGAATGGTGGTCT
NM_014862.3       ACTGAAGTGTTCCAGGACATGCTGCCCATGCCAGGAGATCCAACCCAGGGGACTGGCAAC
                  ******************************************.  .* *..:   * :. 

HsCD00329613      CCCCACTCCCGGCAGCACTTTAGGCAGCCCATAAGCTATGCGAGAATGTTG---
NM_014862.3       TATAACATCGAAGACTTTGCCGACCTGGGCATGTTTCCACCGTTTTCTGAGTAG
                   . .**: * .. *  :    .. *:*  ***.:   .: **: ::   :*   

Clone

Cloning Information : 269115
HIP Master Clone ID : 141837
Original Clone ID : FLH269115.01L

Organism


TAX_ID : 9606
Species Specific ID: 9915

Genome

Chromosome : 15
Map Location : 15q24
Ensembl : ENSG00000172379

Reagents

Labome : ARNT2-antibody