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ACCN1 (Homo sapiens) in pLenti6.2/V5-DEST (V5-tagged lentiviral mammalian expression vector)

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Explanation of Terms


Gene: Gene Symbol:  ASIC2
Gene Name:  acid-sensing (proton-gated) ion channel 2
Sequence             
Original Clone ID: FLH268066.01L
Keyword: None
Species: Homo sapiens
Type: cDNA
Vector Name: pLenti6.2/V5-DEST               Format:  FUSION
Source: HIP
Description: None
Comments: None
Mutation/
Discrepancy :
No / No
Publications: None
Authors:
HsCD00329617

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AVAILABLE
No restriction
Special MTA: None

 

Insert sequence: 1539nts         Open reading frame : 1 to 1539
Reference Sequence Alignment

ATGGACCTCAAGGAAAGCCCCAGTGAGGGCAGCCTGCAACCTTCTAGCATCCAGATCTTT
GCCAACACCTCCACCCTCCATGGCATCCGCCACATCTTCGTGTATGGGCCGCTGACCATC
CGGCGTGTGCTGTGGGCAGTGGCCTTCGTGGGCTCTCTGGGCCTGCTGCTGGTGGAGAGC
TCTGAGAGGGTGTCCTACTACTTCTCCTACCAGCATGTCACTAAGGTGGACGAAGTGGTG
GCTCAAAGCCTGGTCTTCCCAGCTGTGACCCTCTGTAACCTCAATGGCTTCCGGTTCTCC
AGGCTCACCACCAACGACCTGTACCATGCTGGGGAGCTGCTGGCCCTGCTGGATGTCAAC
CTGCAGATCCCGGACCCCCATCTGGCTGACCCCTCCGTGCTGGAGGCCCTGCGGCAGAAG
GCCAACTTCAAGCACTACAAACCCAAGCAGTTCAGCATGCTGGAGTTCCTGCACCGTGTG
GGCCATGACCTGAAGGATATGATGCTCTACTGCAAGTTCAAAGGGCAGGAGTGCGGCCAC
CAAGACTTCACCACAGTGTTTACAAAATATGGGAAGTGTTACATGTTTAACTCAGGCGAG
GATGGCAAACCTCTGCTCACCACGGTCAAGGGGGGGACAGGCAACGGGCTGGAGATCATG
CTGGACATTCAGCAGGATGAGTACCTGCCCATCTGGGGAGAGACAGAGGAAACGACATTT
GAAGCAGGAGTGAAAGTTCAGATCCACAGTCAGTCTGAGCCACCTTTCATCCAAGAGCTG
GGCTTTGGGGTGGCTCCAGGGTTCCAGACCTTTGTGGCCACACAGGAGCAGAGGCTCACA
TACCTGCCCCCACCGTGGGGTGAGTGCCGATCCTCAGAGATGGGCCTCGACTTTTTTCCT
GTTTACAGCATCACCGCCTGTAGGATTGACTGTGAGACCCGCTACATTGTGGAAAACTGC
AACTGCCGCATGGTTCACATGCCAGGGGATGCCCCTTTTTGTACCCCTGAGCAGCACAAG
GAGTGTGCAGAGCCTGCCCTAGGTCTGTTGGCGGAAAAGGACAGCAATTACTGTCTCTGC
AGGACACCCTGCAACCTAACCCGCTACAACAAAGAGCTCTCCATGGTGAAGATCCCCAGC
AAGACATCAGCCAAGTACCTTGAGAAGAAATTTAACAAATCAGAAAAATATATCTCAGAG
AACATCCTTGTTCTGGATATATTTTTTGAAGCTCTCAATTATGAGACAATTGAACAGAAG
AAGGCGTATGAAGTTGCTGCCTTACTTGGTGATATTGGTGGTCAGATGGGATTGTTCATT
GGTGCTAGTATCCTTACAATACTAGAGCTCTTTGATTATATTTATGAGCTGATCAAAGAG
AAGCTATTAGACCTGCTTGGCAAAGAGGAGGACGAAGGGAGCCACGATGAGAATGTGAGT
ACTTGTGACACAATGCCAAACCACTCTGAAACCATCAGTCACACTGTGAACGTGCCCCTG
CAGACGACCCTGGGGACCTTGGAGGAGATTGCCTGCTTG


Coding Sequence Details
Insert Sequence Verified?: N Verification Method: Insert was fully sequenced in parent vector
Reference Sequence Annotations:
End on reference sequence 1580
Start on reference sequence 42
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
5p GTACAAAAAAGCAGGCTCCACC(atg-goi)

3' Linker Sequence:
5p (goi-stop)GACCCAGCTTTCTTGTAC

 

 Recommended Growth Condition:

Distributed in bacterial strain : DH5-alpha T1 phage resistant
Antibiotic Selection: Host Type:  bacterial    Marker:  ampicillin
Host Type:  mammalian    Marker:  blasticidin
Bacterial Selection Condition:  100 ug/mL ampicillin
Growth Condition:  Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:  Commonly used conditions for ampicillin resistant plasmid clones.
Protein Expression Results: None
Recommended expression in: Not Applicable
       DyNA Vector Map

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Vector Name: pLenti6.2/V5-DEST

pLenti6.2V5-DEST in advanced viewed

Synonyms: pLenti6.2/V5-DEST
Sequencing Primer: Forward:  CMV forward
Reverse:  V5 reverse
Description: Lentiviral mammalian expression vector;CMV promoter and C-terminal V5 tag; ampicillin resistance in bacteria; blasticidin resistance in mammalian cells; Gateway recombinational cloning.
Comments: None
Size (bp): 8813
Parent Vector: None
Empty Vector: None
Properties: Gateway, acceptor (destination), lentiviral infection, mammalian expression, recombinational cloning, with tag/fusion/marker
Author Name: Invitrogen
Publications: None
Vector Map:         Vector Sequence:
Sequence:

 



Vector Features:

Type Name Description Start Position End Position
bacterial origin pUC ori pUC origin of replication 7733 8406
death cassette ccdB ccdB death cassette (removed when insert is present) 3767 3976
packaging signal HIV-1 psi HIV-1 psi packaging signal 521 565
poly-A signal SV40 SV40 polyadenylation signal 5639 5770
promoter RSV RSV/5' LTR hybrid promoter 1 410
promoter CMV CMV promoter driving gene of interest 1809 2392
promoter PGK PGK promoter to drive selection marker in mammalian cells 4266 4771
recombination site attR2 attR2 recombinational cloning site 4127 4142
recombination site attR1 attR1 recombinational cloning site 2440 2456
response element HIV-1 RRE HIV-1 Rev response element (RRE) 1075 1308
selectable marker AmpR Ampicillin resistance gene for selection in bacteria 6728 7588
selectable marker BlastR Blasticidin resistance gene in mammalian cells 4849 5249
selectable marker CmR chloramphenicol resistance marker (removed in plasmids with insert) 2673 3329
splice acceptor site 3' splice acceptor two 3' splice acceptors at 1656 and 1684 1656 1656
splice donor site 5' splice donor 5' splice donor 520 520
tag V5 C-terminal V5 epitope tag 4197 4238

 

  • Gene
  • Pathways
  • Protein
  • Clone
  • Organism
  • Genome
  • Reagents

Gene

 

Gene Symbol : ASIC2
Symbol Nomenclature : ASIC2
SYNONYM : ACCN; ACCN1; ASIC2a; BNC1; BNaC1; MDEG; hBNaC1
Designation : acid-sensing ion channel 2|amiloride-sensitive cation channel 1, neuronal|brain sodium channel 1|mammalian degenerin homolog|neuronal amiloride-sensitive cation channel 1
Full Nomenclature : acid-sensing (proton-gated) ion channel 2
GENEID : 40
HGNC : 99
MIM : 601784
Vega : OTTHUMG00000132885
Target GenBank: BC075043

 

Reference Sequence Alignment

  

HsCD00329617      ATGGACCTCAAGGAAAGCCCCAGTGAGGGCAGCCTGCAACCTTCTAGCATCCAGATCTTT
NM_001094.4       ATGGACCTCAAGGAAAGCCCCAGTGAGGGCAGCCTGCAACCTTCTAGCATCCAGATCTTT
                  ************************************************************

HsCD00329617      GCCAACACCTCCACCCTCCATGGCATCCGCCACATCTTCGTGTATGGGCCGCTGACCATC
NM_001094.4       GCCAACACCTCCACCCTCCATGGCATCCGCCACATCTTCGTGTATGGGCCGCTGACCATC
                  ************************************************************

HsCD00329617      CGGCGTGTGCTGTGGGCAGTGGCCTTCGTGGGCTCTCTGGGCCTGCTGCTGGTGGAGAGC
NM_001094.4       CGGCGTGTGCTGTGGGCAGTGGCCTTCGTGGGCTCTCTGGGCCTGCTGCTGGTGGAGAGC
                  ************************************************************

HsCD00329617      TCTGAGAGGGTGTCCTACTACTTCTCCTACCAGCATGTCACTAAGGTGGACGAAGTGGTG
NM_001094.4       TCTGAGAGGGTGTCCTACTACTTCTCCTACCAGCATGTCACTAAGGTGGACGAAGTGGTG
                  ************************************************************

HsCD00329617      GCTCAAAGCCTGGTCTTCCCAGCTGTGACCCTCTGTAACCTCAATGGCTTCCGGTTCTCC
NM_001094.4       GCTCAAAGCCTGGTCTTCCCAGCTGTGACCCTCTGTAACCTGAATGGCTTCCGGTTCTCC
                  ***************************************** ******************

HsCD00329617      AGGCTCACCACCAACGACCTGTACCATGCTGGGGAGCTGCTGGCCCTGCTGGATGTCAAC
NM_001094.4       AGGCTCACCACCAATGACCTGTACCATGCTGGGGAGCTGCTGGCCCTGCTGGATGTCAAC
                  ************** *********************************************

HsCD00329617      CTGCAGATCCCGGACCCCCATCTGGCTGACCCCTCCGTGCTGGAGGCCCTGCGGCAGAAG
NM_001094.4       CTGCAGATCCCGGACCCCCATCTGGCTGACCCCTCCGTGCTGGAGGCCCTGCGGCAGAAG
                  ************************************************************

HsCD00329617      GCCAACTTCAAGCACTACAAACCCAAGCAGTTCAGCATGCTGGAGTTCCTGCACCGTGTG
NM_001094.4       GCCAACTTCAAGCACTACAAACCCAAGCAGTTCAGCATGCTGGAGTTCCTGCACCGTGTG
                  ************************************************************

HsCD00329617      GGCCATGACCTGAAGGATATGATGCTCTACTGCAAGTTCAAAGGGCAGGAGTGCGGCCAC
NM_001094.4       GGCCATGACCTGAAGGATATGATGCTCTACTGCAAGTTCAAAGGGCAGGAGTGCGGCCAC
                  ************************************************************

HsCD00329617      CAAGACTTCACCACAGTGTTTACAAAATATGGGAAGTGTTACATGTTTAACTCAGGCGAG
NM_001094.4       CAAGACTTCACCACAGTGTTTACAAAATATGGGAAGTGTTACATGTTTAACTCAGGCGAG
                  ************************************************************

HsCD00329617      GATGGCAAACCTCTGCTCACCACGGTCAAGGGGGGGACAGGCAACGGGCTGGAGATCATG
NM_001094.4       GATGGCAAACCTCTGCTCACCACGGTCAAGGGGGGGACAGGCAACGGGCTGGAGATCATG
                  ************************************************************

HsCD00329617      CTGGACATTCAGCAGGATGAGTACCTGCCCATCTGGGGAGAGACAGAGGAAACGACATTT
NM_001094.4       CTGGACATTCAGCAGGATGAGTACCTGCCCATCTGGGGAGAGACAGAGGAAACGACATTT
                  ************************************************************

HsCD00329617      GAAGCAGGAGTGAAAGTTCAGATCCACAGTCAGTCTGAGCCACCTTTCATCCAAGAGCTG
NM_001094.4       GAAGCAGGAGTGAAAGTTCAGATCCACAGTCAGTCTGAGCCACCTTTCATCCAAGAGCTG
                  ************************************************************

HsCD00329617      GGCTTTGGGGTGGCTCCAGGGTTCCAGACCTTTGTGGCCACACAGGAGCAGAGGCTCACA
NM_001094.4       GGCTTTGGGGTGGCTCCAGGGTTCCAGACCTTTGTGGCCACACAGGAGCAGAGGCTCACA
                  ************************************************************

HsCD00329617      TACCTGCCCCCACCGTGGGGTGAGTGCCGATCCTCAGAGATGGGCCTCGACTTTTTTCCT
NM_001094.4       TACCTGCCCCCACCGTGGGGTGAGTGCCGATCCTCAGAGATGGGCCTCGACTTTTTTCCT
                  ************************************************************

HsCD00329617      GTTTACAGCATCACCGCCTGTAGGATTGACTGTGAGACCCGCTACATTGTGGAAAACTGC
NM_001094.4       GTTTACAGCATCACCGCCTGTAGGATTGACTGTGAGACCCGCTACATTGTGGAAAACTGC
                  ************************************************************

HsCD00329617      AACTGCCGCATGGTTCACATGCCAGGGGATGCCCCTTTTTGTACCCCTGAGCAGCACAAG
NM_001094.4       AACTGCCGCATGGTTCACATGCCAGGGGATGCCCCTTTTTGTACCCCTGAGCAGCACAAG
                  ************************************************************

HsCD00329617      GAGTGTGCAGAGCCTGCCCTAGGTCTGTTGGCGGAAAAGGACAGCAATTACTGTCTCTGC
NM_001094.4       GAGTGTGCAGAGCCTGCCCTAGGTCTGTTGGCGGAAAAGGACAGCAATTACTGTCTCTGC
                  ************************************************************

HsCD00329617      AGGACACCCTGCAACCTAACCCGCTACAACAAAGAGCTCTCCATGGTGAAGATCCCCAGC
NM_001094.4       AGGACACCCTGCAACCTAACCCGCTACAACAAAGAGCTCTCCATGGTGAAGATCCCCAGC
                  ************************************************************

HsCD00329617      AAGACATCAGCCAAGTACCTTGAGAAGAAATTTAACAAATCAGAAAAATATATCTCAGAG
NM_001094.4       AAGACATCAGCCAAGTACCTTGAGAAGAAATTTAACAAATCAGAAAAATATATCTCAGAG
                  ************************************************************

HsCD00329617      AACATCCTTGTTCTGGATATATTTTTTGAAGCTCTCAATTATGAGACAATTGAACAGAAG
NM_001094.4       AACATCCTTGTTCTGGATATATTTTTTGAAGCTCTCAATTATGAGACAATTGAACAGAAG
                  ************************************************************

HsCD00329617      AAGGCGTATGAAGTTGCTGCCTTACTTGGTGATATTGGTGGTCAGATGGGATTGTTCATT
NM_001094.4       AAGGCGTATGAAGTTGCTGCCTTACTTGGTGATATTGGTGGTCAGATGGGATTGTTCATT
                  ************************************************************

HsCD00329617      GGTGCTAGTATCCTTACAATACTAGAGCTCTTTGATTATATTTATGAGCTGATCAAAGAG
NM_001094.4       GGTGCTAGTATCCTTACAATACTAGAGCTCTTTGATTATATTTATGAGCTGATCAAAGAG
                  ************************************************************

HsCD00329617      AAGCTATTAGACCTGCTTGGCAAAGAGGAGGACGAAGGGAGCCACGATGAGAATGTGAGT
NM_001094.4       AAGCTATTAGACCTGCTTGGCAAAGAGGAGGACGAAGGGAGCCACGATGAGAATGTGAGT
                  ************************************************************

HsCD00329617      ACTTGTGACACAATGCCAAACCACTCTGAAACCATCAGTCACACTGTGAACGTGCCCCTG
NM_001094.4       ACTTGTGACACAATGCCAAACCACTCTGAAACCATCAGTCACACTGTGAACGTGCCCCTG
                  ************************************************************

HsCD00329617      CAGACGACCCTGGGGACCTTGGAGGAGATTGCCTGCTTG
NM_001094.4       CAGACGACCCTGGGGACCTTGGAGGAGATTGCCTGCTGA
                  ************************************* .

Pathways

Reactome : Ion channel transport
Reactome : Stimuli-sensing channels
Reactome : Transmembrane transport of small molecules

Protein

UniProt : E9PBX2
UniProt : Q16515
HPRD : 03471

Clone

Cloning Information : 268066
HIP Master Clone ID : 193096
Original Clone ID : FLH268066.01L

Organism


TAX_ID : 9606
Species Specific ID: 40

Genome

Chromosome : 17
Map Location : 17q12
Ensembl : ENSG00000108684

Reagents

Labome : ASIC2-antibody