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ZNF449 (Homo sapiens) in pLenti6.2/V5-DEST (V5-tagged lentiviral mammalian expression vector)

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Explanation of Terms


Gene: Gene Symbol:  ZNF449
Gene Name:  zinc finger protein 449
Sequence             
Original Clone ID: FLH268068.01L
Keyword: None
Species: Homo sapiens
Type: cDNA
Vector Name: pLenti6.2/V5-DEST               Format:  FUSION
Source: HIP
Description: None
Comments: None
Mutation/
Discrepancy :
No / No
Publications: None
Authors:
HsCD00329619

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AVAILABLE
No restriction
Special MTA: None

 

Insert sequence: 1557nts         Open reading frame : 1 to 1557
Reference Sequence Alignment

ATGGCTGTGGCCCTGGGTTGTGCAATCCAGGCATCCTTGAATCAAGGCTCTGTGTTTCAA
GAATATGATACTGACTGTGAAGTTTTCCGTCAGCGCTTCAGGCAGTTCCAGTACAGAGAA
GCAGCTGGGCCTCATGAAGCATTTAACAAACTCTGGGAGCTTTGCTGTCAATGGCTGAAG
CCAAAGATGCGCTCTAAGGAACAAATCCTGGAGCTGCTAGTGTTGGAGCAATTCCTAACT
ATCCTGCCCACAGAGATAGAGACCTGGGTGAGGGAGCACTGCCCAGAGAATAGAGAAAGA
GTTGTGTCACTGATAGAAGACTTACAGAGAGAACTTGAGATACCAGAGCAGCAGGTTGAT
ATGCATGACATGCTCTTGGAAGAACTGGCACCAGTGGGAACGGCACACATACCACCAACC
ATGCACCTAGAGTCACCTGCACTCCAGGTAATGGGACCTGCCCAGGAGGCCCCAGTAGCA
GAGGCATGGATCCCACAGGCAGGGCCACCGGAGCTGAACTATGGTGCTACTGGAGAATGT
CAGAACTTTCTGGACCCTGGATATCCATTACCAAAACTTGACATGAACTTCTCATTGGAG
AATAGAGAAGAGCCATGGGTGAAGGAATTACAGGATTCTAAAGAAATGAAACAATTACTT
GATTCCAAGATAGGTTTTGAGATCGGGATAGAAAATGAAGAAGATACTTCAAAACAGAAA
AAAATGGAGACTATGTATCCATTTATTGTAACTTTAGAGGGGAATGCTCTCCAGGGTCCC
ATTTTGCAAAAAGACTATGTACAGTTAGAAAATCAATGGGAAACCCCCCCAGAGGATTTA
CAGACAGATTTAGCAAAACTGGTAGATCAGCAGAACCCCACTCTGGGAGAGACACCTGAG
AACTCCAACTTGGAAGAACCTCTCAACCCTAAACCCCACAAGAAAAAGAGTCCAGGAGAG
AAACCTCACCGATGTCCTCAGTGTGGAAAATGTTTTGCTCGGAAGTCACAACTTACTGGG
CATCAGAGAATTCATTCAGGAGAAGAACCTCACAAATGCCCTGAATGTGGGAAAAGATTC
CTTCGTAGTTCAGACCTTTATAGACACCAACGACTTCATACAGGGGAGAGACCCTATGAA
TGCACTGTATGTAAAAAGCGATTCACTCGGCGGTCACATCTTATAGGGCACCAGAGAACC
CATTCTGAAGAAGAAACATATAAATGTCTTGAGTGTGGGAAAAGTTTTTGTCATGGATCA
AGTCTTAAAAGACATCTGAAAACTCATACAGGTGAAAAACCTCATAGATGTCATAATTGT
GGGAAAAGTTTTAGTCGACTGACAGCTCTTACTTTGCACCAGAGAACGCATACTGAAGAG
AGACCTTTTAAATGTAATTATTGTGGGAAAAGTTTTAGACAGAGACCAAGCCTCGTTATT
CATTTAAGAATCCACACAGGGGAGAAGCCATACAAGTGTACTCATTGTTCTAAAAGCTTC
AGACAGAGAGCCGGCCTTATTATGCACCAGGTCACTCACTTTAGAGGACTTATTTTG


Coding Sequence Details
Insert Sequence Verified?: N Verification Method: Insert was fully sequenced in parent vector
Reference Sequence Annotations:
End on reference sequence 1697
Start on reference sequence 141
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
5p GTACAAAAAAGCAGGCTCCACC(atg-goi)

3' Linker Sequence:
5p (goi-stop)GACCCAGCTTTCTTGTAC

 

 Recommended Growth Condition:

Distributed in bacterial strain : DH5-alpha T1 phage resistant
Antibiotic Selection: Host Type:  bacterial    Marker:  ampicillin
Host Type:  mammalian    Marker:  blasticidin
Bacterial Selection Condition:  100 ug/mL ampicillin
Growth Condition:  Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:  Commonly used conditions for ampicillin resistant plasmid clones.
Protein Expression Results: None
Recommended expression in: Not Applicable
       DyNA Vector Map

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Vector Name: pLenti6.2/V5-DEST

pLenti6.2V5-DEST in advanced viewed

Synonyms: pLenti6.2/V5-DEST
Sequencing Primer: Forward:  CMV forward
Reverse:  V5 reverse
Description: Lentiviral mammalian expression vector;CMV promoter and C-terminal V5 tag; ampicillin resistance in bacteria; blasticidin resistance in mammalian cells; Gateway recombinational cloning.
Comments: None
Size (bp): 8813
Parent Vector: None
Empty Vector: None
Properties: Gateway, acceptor (destination), lentiviral infection, mammalian expression, recombinational cloning, with tag/fusion/marker
Author Name: Invitrogen
Publications: None
Vector Map:         Vector Sequence:
Sequence:

 



Vector Features:

Type Name Description Start Position End Position
bacterial origin pUC ori pUC origin of replication 7733 8406
death cassette ccdB ccdB death cassette (removed when insert is present) 3767 3976
packaging signal HIV-1 psi HIV-1 psi packaging signal 521 565
poly-A signal SV40 SV40 polyadenylation signal 5639 5770
promoter RSV RSV/5' LTR hybrid promoter 1 410
promoter CMV CMV promoter driving gene of interest 1809 2392
promoter PGK PGK promoter to drive selection marker in mammalian cells 4266 4771
recombination site attR2 attR2 recombinational cloning site 4127 4142
recombination site attR1 attR1 recombinational cloning site 2440 2456
response element HIV-1 RRE HIV-1 Rev response element (RRE) 1075 1308
selectable marker AmpR Ampicillin resistance gene for selection in bacteria 6728 7588
selectable marker BlastR Blasticidin resistance gene in mammalian cells 4849 5249
selectable marker CmR chloramphenicol resistance marker (removed in plasmids with insert) 2673 3329
splice acceptor site 3' splice acceptor two 3' splice acceptors at 1656 and 1684 1656 1656
splice donor site 5' splice donor 5' splice donor 520 520
tag V5 C-terminal V5 epitope tag 4197 4238

 

  • Gene
  • Protein
  • Clone
  • Organism
  • Genome
  • Reagents

Gene

 

Gene Symbol : ZNF449
Symbol Nomenclature : ZNF449
SYNONYM : ZSCAN19
Designation : zinc finger and SCAN domain-containing protein 19
Full Nomenclature : zinc finger protein 449
GENEID : 203523
HGNC : 21039
MIM : 300627
Vega : OTTHUMG00000022478
Target GenBank: BC060768

 

Reference Sequence Alignment

  

HsCD00329619      ATGGCTGTGGCCCTGGGTTGTGCAATCCAGGCATCCTTGAATCAAGGCTCTGTGTTTCAA
NM_152695.5       ATGGCTGTGGCCCTGGGTTGTGCAATCCAGGCATCCTTGAATCAAGGCTCTGTGTTTCAA
                  ************************************************************

HsCD00329619      GAATATGATACTGACTGTGAAGTTTTCCGTCAGCGCTTCAGGCAGTTCCAGTACAGAGAA
NM_152695.5       GAATATGATACTGACTGTGAAGTTTTCCGTCAGCGCTTCAGGCAGTTCCAGTACAGAGAA
                  ************************************************************

HsCD00329619      GCAGCTGGGCCTCATGAAGCATTTAACAAACTCTGGGAGCTTTGCTGTCAATGGCTGAAG
NM_152695.5       GCAGCTGGGCCTCATGAAGCATTTAACAAACTCTGGGAGCTTTGCTGTCAATGGCTGAAG
                  ************************************************************

HsCD00329619      CCAAAGATGCGCTCTAAGGAACAAATCCTGGAGCTGCTAGTGTTGGAGCAATTCCTAACT
NM_152695.5       CCAAAGATGCGCTCTAAGGAACAAATCCTGGAGCTGCTAGTGTTGGAGCAATTCCTAACT
                  ************************************************************

HsCD00329619      ATCCTGCCCACAGAGATAGAGACCTGGGTGAGGGAGCACTGCCCAGAGAATAGAGAAAGA
NM_152695.5       ATCCTGCCCACAGAGATAGAGACCTGGGTGAGGGAGCACTGCCCAGAGAATAGAGAAAGA
                  ************************************************************

HsCD00329619      GTTGTGTCACTGATAGAAGACTTACAGAGAGAACTTGAGATACCAGAGCAGCAGGTTGAT
NM_152695.5       GTTGTGTCACTGATAGAAGACTTACAGAGAGAACTTGAGATACCAGAGCAGCAGGTTGAT
                  ************************************************************

HsCD00329619      ATGCATGACATGCTCTTGGAAGAACTGGCACCAGTGGGAACGGCACACATACCACCAACC
NM_152695.5       ATGCATGACATGCTCTTGGAAGAACTGGCACCAGTGGGAACGGCACACATACCACCAACC
                  ************************************************************

HsCD00329619      ATGCACCTAGAGTCACCTGCACTCCAGGTAATGGGACCTGCCCAGGAGGCCCCAGTAGCA
NM_152695.5       ATGCACCTAGAGTCACCTGCACTCCAGGTAATGGGACCTGCCCAGGAGGCCCCAGTAGCA
                  ************************************************************

HsCD00329619      GAGGCATGGATCCCACAGGCAGGGCCACCGGAGCTGAACTATGGTGCTACTGGAGAATGT
NM_152695.5       GAGGCATGGATCCCACAGGCAGGGCCACCGGAGCTGAACTATGGTGCTACTGGAGAATGT
                  ************************************************************

HsCD00329619      CAGAACTTTCTGGACCCTGGATATCCATTACCAAAACTTGACATGAACTTCTCATTGGAG
NM_152695.5       CAGAACTTTCTGGACCCTGGATATCCATTACCAAAACTTGACATGAACTTCTCATTGGAG
                  ************************************************************

HsCD00329619      AATAGAGAAGAGCCATGGGTGAAGGAATTACAGGATTCTAAAGAAATGAAACAATTACTT
NM_152695.5       AATAGAGAAGAGCCATGGGTGAAGGAATTACAGGATTCTAAAGAAATGAAACAATTACTT
                  ************************************************************

HsCD00329619      GATTCCAAGATAGGTTTTGAGATCGGGATAGAAAATGAAGAAGATACTTCAAAACAGAAA
NM_152695.5       GATTCCAAGATAGGTTTTGAGATCGGGATAGAAAATGAAGAAGATACTTCAAAACAGAAA
                  ************************************************************

HsCD00329619      AAAATGGAGACTATGTATCCATTTATTGTAACTTTAGAGGGGAATGCTCTCCAGGGTCCC
NM_152695.5       AAAATGGAGACTATGTATCCATTTATTGTAACTTTAGAGGGGAATGCTCTCCAGGGTCCC
                  ************************************************************

HsCD00329619      ATTTTGCAAAAAGACTATGTACAGTTAGAAAATCAATGGGAAACCCCCCCAGAGGATTTA
NM_152695.5       ATTTTGCAAAAAGACTATGTACAGTTAGAAAATCAATGGGAAACCCCCCCAGAGGATTTA
                  ************************************************************

HsCD00329619      CAGACAGATTTAGCAAAACTGGTAGATCAGCAGAACCCCACTCTGGGAGAGACACCTGAG
NM_152695.5       CAGACAGATTTAGCAAAACTGGTAGATCAGCAGAACCCCACTCTGGGAGAGACACCTGAG
                  ************************************************************

HsCD00329619      AACTCCAACTTGGAAGAACCTCTCAACCCTAAACCCCACAAGAAAAAGAGTCCAGGAGAG
NM_152695.5       AACTCCAACTTGGAAGAACCTCTCAACCCTAAACCCCACAAGAAAAAGAGTCCAGGAGAG
                  ************************************************************

HsCD00329619      AAACCTCACCGATGTCCTCAGTGTGGAAAATGTTTTGCTCGGAAGTCACAACTTACTGGG
NM_152695.5       AAACCTCACCGATGTCCTCAGTGTGGAAAATGTTTTGCTCGGAAGTCACAACTTACTGGG
                  ************************************************************

HsCD00329619      CATCAGAGAATTCATTCAGGAGAAGAACCTCACAAATGCCCTGAATGTGGGAAAAGATTC
NM_152695.5       CATCAGAGAATTCATTCAGGAGAAGAACCTCACAAATGCCCTGAATGTGGGAAAAGATTC
                  ************************************************************

HsCD00329619      CTTCGTAGTTCAGACCTTTATAGACACCAACGACTTCATACAGGGGAGAGACCCTATGAA
NM_152695.5       CTTCGTAGTTCAGACCTTTATAGACACCAACGACTTCATACAGGGGAGAGACCCTATGAA
                  ************************************************************

HsCD00329619      TGCACTGTATGTAAAAAGCGATTCACTCGGCGGTCACATCTTATAGGGCACCAGAGAACC
NM_152695.5       TGCACTGTATGTAAAAAGCGATTCACTCGGCGGTCACATCTTATAGGGCACCAGAGAACC
                  ************************************************************

HsCD00329619      CATTCTGAAGAAGAAACATATAAATGTCTTGAGTGTGGGAAAAGTTTTTGTCATGGATCA
NM_152695.5       CATTCTGAAGAAGAAACATATAAATGTCTTGAGTGTGGGAAAAGTTTTTGTCATGGATCA
                  ************************************************************

HsCD00329619      AGTCTTAAAAGACATCTGAAAACTCATACAGGTGAAAAACCTCATAGATGTCATAATTGT
NM_152695.5       AGTCTTAAAAGACATCTGAAAACTCATACAGGTGAAAAACCTCATAGATGTCATAATTGT
                  ************************************************************

HsCD00329619      GGGAAAAGTTTTAGTCGACTGACAGCTCTTACTTTGCACCAGAGAACGCATACTGAAGAG
NM_152695.5       GGGAAAAGTTTTAGTCGACTGACAGCTCTTACTTTGCACCAGAGAACGCATACTGAAGAG
                  ************************************************************

HsCD00329619      AGACCTTTTAAATGTAATTATTGTGGGAAAAGTTTTAGACAGAGACCAAGCCTCGTTATT
NM_152695.5       AGACCTTTTAAATGTAATTATTGTGGGAAAAGTTTTAGACAGAGACCAAGCCTCGTTATT
                  ************************************************************

HsCD00329619      CATTTAAGAATCCACACAGGGGAGAAGCCATACAAGTGTACTCATTGTTCTAAAAGCTTC
NM_152695.5       CATTTAAGAATCCACACAGGGGAGAAGCCATACAAGTGTACTCATTGTTCTAAAAGCTTC
                  ************************************************************

HsCD00329619      AGACAGAGAGCCGGCCTTATTATGCACCAGGTCACTCACTTTAGAGGACTTATTTTG
NM_152695.5       AGACAGAGAGCCGGCCTTATTATGCACCAGGTCACTCACTTTAGAGGACTTATTTAA
                  *******************************************************:.

Protein

SMART domain : ZnF_C2H2 : zinc finger
SMART domain : SCAN : leucine rich region
UniProt : Q7Z3P1
UniProt : Q6P9G9
HPRD : 06543

Clone

Cloning Information : 268068
HIP Master Clone ID : 193098
Original Clone ID : FLH268068.01L

Organism


TAX_ID : 9606
Species Specific ID: 203523

Genome

Chromosome : X
Map Location : Xq26.3
Ensembl : ENSG00000173275

Reagents

Labome : ZNF449-antibody