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ZNF623 (Homo sapiens) in pLenti6.2/V5-DEST (V5-tagged lentiviral mammalian expression vector)

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Explanation of Terms


Gene: Gene Symbol:  ZNF623
Gene Name:  zinc finger protein 623
Sequence             
Original Clone ID: FLH268069.01L
Keyword: None
Species: Homo sapiens
Type: cDNA
Vector Name: pLenti6.2/V5-DEST               Format:  FUSION
Source: HIP
Description: None
Comments: None
Mutation/
Discrepancy :
No / No
Publications: None
Authors:
HsCD00329620

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AVAILABLE
No restriction
Special MTA: None

 

Insert sequence: 1611nts         Open reading frame : 1 to 1611
Reference Sequence Alignment

ATGATTTTGTTGTCTTTTGTTTCAGATTCTAATGTAGGAACTGGTGAGAAGAAGGTGACT
GAAGCCTGGATTTCTGAGGATGAAAACTCACATAGGACGACGTCAGACAGACTCACGGTG
ATGGAGCTCCCCTCTCCCGAGTCTGAGGAAGTCCACGAGCCCAGATTAGGGGAGCTCTTG
GGAAATCCAGAAGGTCAGAGCCTGGGGAGTTCCCCCTCTCAGGACAGGGGCTGCAAGCAG
GTGACAGTGACCCATTGGAAGATCCAGACAGGAGAGACAGCTCAAGTGTGCACCAAGTCA
GGAAGAAACCATATTCTGAACTCAGACCTTCTTCTGCTTCAGAGAGAGCTCATAGAGGGG
GAAGCCAATCCTTGCGATATCTGTGGCAAAACCTTCACGTTTAATTCGGACCTAGTTAGG
CATCGGATTTCGCATGCTGGGGAGAAACCTTACACGTGCGATCAGTGTGGGAAAGGCTTT
GGCCAGAGCTCACACCTTATGGAGCATCAGAGAATTCACACTGGAGAGAGACTCTACGTC
TGTAATGTGTGTGGGAAAGACTTCATTCACTATTCAGGTCTCATTGAGCATCAGCGCGTT
CATTCAGGAGAAAAGCCCTTCAAATGTGCGCAGTGTGGGAAGGCGTTTTGTCACAGTTCA
GACCTGATTAGGCACCAGAGAGTTCACACCAGAGAGAGACCTTTTGAATGCAAAGAGTGT
GGGAAAGGCTTCAGTCAGAGCTCCTTACTTATTCGCCATCAGAGGATTCACACGGGAGAA
AGGCCCTATGAGTGCAATGAATGTGGGAAATCCTTCATAAGGAGCTCGAGCCTCATTCGC
CATTATCAGATCCACACAGAAGTGAAACAGTATGAATGCAAAGAATGTGGGAAGGCATTC
CGTCATCGCTCAGACCTTATTGAACACCAGAGAATTCACACCGGAGAGAGACCCTTTGAA
TGCAATGAGTGTGGGAAAGCCTTTATTCGGAGTTCAAAGCTCATTCAGCATCAGAGGATC
CATACTGGGGAGAGGCCTTACGTATGCAATGAGTGTGGGAAGCGCTTCAGCCAGACGTCA
AACTTCACCCAGCATCAGAGAATTCACACTGGAGAGAAACTCTATGAATGTAACGAGTGT
GGGAAAGCTTTCTTTCTGAGTTCATACCTTATTCGACACCAGAAAATCCACACTGGAGAG
AGAGTGTATGAATGTAAGGAATGTGGGAAAGCGTTTCTCCAGAAAGCCCATCTCACTGAG
CACCAGAAGATCCACTCTGGGGACAGGCCCTTCGAATGTAAAGACTGTGGGAAAGCCTTC
ATCCAGAGCTCCAAGCTGCTTCTGCACCAGATTATTCACACTGGAGAAAAGCCCTATGTG
TGCAGTTATTGTGGGAAAGGCTTTATTCAGAGGTCAAACTTCCTTCAACACCAGAAAATT
CATACTGAAGAGAAGCTCTATGAATGTAGTCAGTATGGGAGAGATTTTAACTCAACTACA
AACGTTAAAAATAATCAAAGGGTTCACCAAGAGGGACTCTCCTTGAGTAAGGCCCCCATA
CATTTGGGTGAGAGGTCTGTAGATAAGGGGGAACACACAGGTAACTTATTG


Coding Sequence Details
Insert Sequence Verified?: N Verification Method: Insert was fully sequenced in parent vector
Reference Sequence Annotations:
End on reference sequence 1647
Start on reference sequence 37
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
5p GTACAAAAAAGCAGGCTCCACC(atg-goi)

3' Linker Sequence:
5p (goi-stop)GACCCAGCTTTCTTGTAC

 

 Recommended Growth Condition:

Distributed in bacterial strain : DH5-alpha T1 phage resistant
Antibiotic Selection: Host Type:  bacterial    Marker:  ampicillin
Host Type:  mammalian    Marker:  blasticidin
Bacterial Selection Condition:  100 ug/mL ampicillin
Growth Condition:  Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:  Commonly used conditions for ampicillin resistant plasmid clones.
Protein Expression Results: None
Recommended expression in: Not Applicable
       DyNA Vector Map

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Vector Name: pLenti6.2/V5-DEST

pLenti6.2V5-DEST in advanced viewed

Synonyms: pLenti6.2/V5-DEST
Sequencing Primer: Forward:  CMV forward
Reverse:  V5 reverse
Description: Lentiviral mammalian expression vector;CMV promoter and C-terminal V5 tag; ampicillin resistance in bacteria; blasticidin resistance in mammalian cells; Gateway recombinational cloning.
Comments: None
Size (bp): 8813
Parent Vector: None
Empty Vector: None
Properties: Gateway, acceptor (destination), lentiviral infection, mammalian expression, recombinational cloning, with tag/fusion/marker
Author Name: Invitrogen
Publications: None
Vector Map:         Vector Sequence:
Sequence:

 



Vector Features:

Type Name Description Start Position End Position
bacterial origin pUC ori pUC origin of replication 7733 8406
death cassette ccdB ccdB death cassette (removed when insert is present) 3767 3976
packaging signal HIV-1 psi HIV-1 psi packaging signal 521 565
poly-A signal SV40 SV40 polyadenylation signal 5639 5770
promoter RSV RSV/5' LTR hybrid promoter 1 410
promoter CMV CMV promoter driving gene of interest 1809 2392
promoter PGK PGK promoter to drive selection marker in mammalian cells 4266 4771
recombination site attR2 attR2 recombinational cloning site 4127 4142
recombination site attR1 attR1 recombinational cloning site 2440 2456
response element HIV-1 RRE HIV-1 Rev response element (RRE) 1075 1308
selectable marker AmpR Ampicillin resistance gene for selection in bacteria 6728 7588
selectable marker BlastR Blasticidin resistance gene in mammalian cells 4849 5249
selectable marker CmR chloramphenicol resistance marker (removed in plasmids with insert) 2673 3329
splice acceptor site 3' splice acceptor two 3' splice acceptors at 1656 and 1684 1656 1656
splice donor site 5' splice donor 5' splice donor 520 520
tag V5 C-terminal V5 epitope tag 4197 4238

 

  • Gene
  • Protein
  • Clone
  • Organism
  • Genome
  • Reagents

Gene

 

Gene Symbol : ZNF623
Symbol Nomenclature : ZNF623
Full Nomenclature : zinc finger protein 623
GENEID : 9831
HGNC : 29084
Vega : OTTHUMG00000165186
Target GenBank: BC075057

 

Reference Sequence Alignment

  

HsCD00329620        ATGATTTTGTTGTCTTTTGTTTCAGATTCTAATGTAGGAACTGGTGAGAAGAAGGTGACT
NM_001261843.1      ------------------------------------------------------------
NM_001082480.2      ------------------------------------------------------------
NM_014789.3         ATGATTTTGTTGTCTTTTGTTTCAGATTCTAATGTAGGAACTGGTGAGAAGAAGGTGACT
                                                                                

HsCD00329620        GAAGCCTGGATTTCTGAGGATGAAAACTCACATAGGACGACGTCAGACAGACTCACGGTG
NM_001261843.1      ------------------------------------------------------------
NM_001082480.2      ------------------------------------------------------------
NM_014789.3         GAAGCCTGGATTTCTGAGGATGAAAACTCACATAGGACGACGTCAGACAGACTCACGGTG
                                                                                

HsCD00329620        ATGGAGCTCCCCTCTCCCGAGTCTGAGGAAGTCCACGAGCCCAGATTAGGGGAGCTCTTG
NM_001261843.1      ATGGAGCTCCCCTCTCCCGAGTCTGAGGAAGTCCACGAGCCCAGATTAGGGGAGCTCTTG
NM_001082480.2      ATGGAGCTCCCCTCTCCCGAGTCTGAGGAAGTCCACGAGCCCAGATTAGGGGAGCTCTTG
NM_014789.3         ATGGAGCTCCCCTCTCCCGAGTCTGAGGAAGTCCACGAGCCCAGATTAGGGGAGCTCTTG
                    ************************************************************

HsCD00329620        GGAAATCCAGAAGGTCAGAGCCTGGGGAGTTCCCCCTCTCAGGACAGGGGCTGCAAGCAG
NM_001261843.1      GGAAATCCAGAAGGTCAGAGCCTGGGGAGTTCCCCCTCTCAGGACAGGGGCTGCAAGCAG
NM_001082480.2      GGAAATCCAGAAGGTCAGAGCCTGGGGAGTTCCCCCTCTCAGGACAGGGGCTGCAAGCAG
NM_014789.3         GGAAATCCAGAAGGTCAGAGCCTGGGGAGTTCCCCCTCTCAGGACAGGGGCTGCAAGCAG
                    ************************************************************

HsCD00329620        GTGACAGTGACCCATTGGAAGATCCAGACAGGAGAGACAGCTCAAGTGTGCACCAAGTCA
NM_001261843.1      GTGACAGTGACCCATTGGAAGATCCAGACAGGAGAGACAGCTCAAGTGTGCACCAAGTCA
NM_001082480.2      GTGACAGTGACCCATTGGAAGATCCAGACAGGAGAGACAGCTCAAGTGTGCACCAAGTCA
NM_014789.3         GTGACAGTGACCCATTGGAAGATCCAGACAGGAGAGACAGCTCAAGTGTGCACCAAGTCA
                    ************************************************************

HsCD00329620        GGAAGAAACCATATTCTGAACTCAGACCTTCTTCTGCTTCAGAGAGAGCTCATAGAGGGG
NM_001261843.1      GGAAGAAACCATATTCTGAACTCAGACCTTCTTCTGCTTCAGAGAGAGCTCATAGAGGGG
NM_001082480.2      GGAAGAAACCATATTCTGAACTCAGACCTTCTTCTGCTTCAGAGAGAGCTCATAGAGGGG
NM_014789.3         GGAAGAAACCATATTCTGAACTCAGACCTTCTTCTGCTTCAGAGAGAGCTCATAGAGGGG
                    ************************************************************

HsCD00329620        GAAGCCAATCCTTGCGATATCTGTGGCAAAACCTTCACGTTTAATTCGGACCTAGTTAGG
NM_001261843.1      GAAGCCAATCCTTGCGATATCTGTGGCAAAACCTTCACGTTTAATTCGGACCTAGTTAGG
NM_001082480.2      GAAGCCAATCCTTGCGATATCTGTGGCAAAACCTTCACGTTTAATTCGGACCTAGTTAGG
NM_014789.3         GAAGCCAATCCTTGCGATATCTGTGGCAAAACCTTCACGTTTAATTCGGACCTAGTTAGG
                    ************************************************************

HsCD00329620        CATCGGATTTCGCATGCTGGGGAGAAACCTTACACGTGCGATCAGTGTGGGAAAGGCTTT
NM_001261843.1      CATCGGATTTCGCATGCTGGGGAGAAACCTTACACGTGCGATCAGTGTGGGAAAGGCTTT
NM_001082480.2      CATCGGATTTCGCATGCTGGGGAGAAACCTTACACGTGCGATCAGTGTGGGAAAGGCTTT
NM_014789.3         CATCGGATTTCGCATGCTGGGGAGAAACCTTACACGTGCGATCAGTGTGGGAAAGGCTTT
                    ************************************************************

HsCD00329620        GGCCAGAGCTCACACCTTATGGAGCATCAGAGAATTCACACTGGAGAGAGACTCTACGTC
NM_001261843.1      GGCCAGAGCTCACACCTTATGGAGCATCAGAGAATTCACACTGGAGAGAGACTCTACGTC
NM_001082480.2      GGCCAGAGCTCACACCTTATGGAGCATCAGAGAATTCACACTGGAGAGAGACTCTACGTC
NM_014789.3         GGCCAGAGCTCACACCTTATGGAGCATCAGAGAATTCACACTGGAGAGAGACTCTACGTC
                    ************************************************************

HsCD00329620        TGTAATGTGTGTGGGAAAGACTTCATTCACTATTCAGGTCTCATTGAGCATCAGCGCGTT
NM_001261843.1      TGTAATGTGTGTGGGAAAGACTTCATTCACTATTCAGGTCTCATTGAGCATCAGCGCGTT
NM_001082480.2      TGTAATGTGTGTGGGAAAGACTTCATTCACTATTCAGGTCTCATTGAGCATCAGCGCGTT
NM_014789.3         TGTAATGTGTGTGGGAAAGACTTCATTCACTATTCAGGTCTCATTGAGCATCAGCGCGTT
                    ************************************************************

HsCD00329620        CATTCAGGAGAAAAGCCCTTCAAATGTGCGCAGTGTGGGAAGGCGTTTTGTCACAGTTCA
NM_001261843.1      CATTCAGGAGAAAAGCCCTTCAAATGTGCGCAGTGTGGGAAGGCGTTTTGTCACAGTTCA
NM_001082480.2      CATTCAGGAGAAAAGCCCTTCAAATGTGCGCAGTGTGGGAAGGCGTTTTGTCACAGTTCA
NM_014789.3         CATTCAGGAGAAAAGCCCTTCAAATGTGCGCAGTGTGGGAAGGCGTTTTGTCACAGTTCA
                    ************************************************************

HsCD00329620        GACCTGATTAGGCACCAGAGAGTTCACACCAGAGAGAGACCTTTTGAATGCAAAGAGTGT
NM_001261843.1      GACCTGATTAGGCACCAGAGAGTTCACACCAGAGAGAGACCTTTTGAATGCAAAGAGTGT
NM_001082480.2      GACCTGATTAGGCACCAGAGAGTTCACACCAGAGAGAGACCTTTTGAATGCAAAGAGTGT
NM_014789.3         GACCTGATTAGGCACCAGAGAGTTCACACCAGAGAGAGACCTTTTGAATGCAAAGAGTGT
                    ************************************************************

HsCD00329620        GGGAAAGGCTTCAGTCAGAGCTCCTTACTTATTCGCCATCAGAGGATTCACACGGGAGAA
NM_001261843.1      GGGAAAGGCTTCAGTCAGAGCTCCTTACTTATTCGCCATCAGAGGATTCACACGGGAGAA
NM_001082480.2      GGGAAAGGCTTCAGTCAGAGCTCCTTACTTATTCGCCATCAGAGGATTCACACGGGAGAA
NM_014789.3         GGGAAAGGCTTCAGTCAGAGCTCCTTACTTATTCGCCATCAGAGGATTCACACGGGAGAA
                    ************************************************************

HsCD00329620        AGGCCCTATGAGTGCAATGAATGTGGGAAATCCTTCATAAGGAGCTCGAGCCTCATTCGC
NM_001261843.1      AGGCCCTATGAGTGCAATGAATGTGGGAAATCCTTCATAAGGAGCTCGAGCCTCATTCGC
NM_001082480.2      AGGCCCTATGAGTGCAATGAATGTGGGAAATCCTTCATAAGGAGCTCGAGCCTCATTCGC
NM_014789.3         AGGCCCTATGAGTGCAATGAATGTGGGAAATCCTTCATAAGGAGCTCGAGCCTCATTCGC
                    ************************************************************

HsCD00329620        CATTATCAGATCCACACAGAAGTGAAACAGTATGAATGCAAAGAATGTGGGAAGGCATTC
NM_001261843.1      CATTATCAGATCCACACAGAAGTGAAACAGTATGAATGCAAAGAATGTGGGAAGGCATTC
NM_001082480.2      CATTATCAGATCCACACAGAAGTGAAACAGTATGAATGCAAAGAATGTGGGAAGGCATTC
NM_014789.3         CATTATCAGATCCACACAGAAGTGAAACAGTATGAATGCAAAGAATGTGGGAAGGCATTC
                    ************************************************************

HsCD00329620        CGTCATCGCTCAGACCTTATTGAACACCAGAGAATTCACACCGGAGAGAGACCCTTTGAA
NM_001261843.1      CGTCATCGCTCAGACCTTATTGAACACCAGAGAATTCACACCGGAGAGAGACCCTTTGAA
NM_001082480.2      CGTCATCGCTCAGACCTTATTGAACACCAGAGAATTCACACCGGAGAGAGACCCTTTGAA
NM_014789.3         CGTCATCGCTCAGACCTTATTGAACACCAGAGAATTCACACCGGAGAGAGACCCTTTGAA
                    ************************************************************

HsCD00329620        TGCAATGAGTGTGGGAAAGCCTTTATTCGGAGTTCAAAGCTCATTCAGCATCAGAGGATC
NM_001261843.1      TGCAATGAGTGTGGGAAAGCCTTTATTCGGAGTTCAAAGCTCATTCAGCATCAGAGGATC
NM_001082480.2      TGCAATGAGTGTGGGAAAGCCTTTATTCGGAGTTCAAAGCTCATTCAGCATCAGAGGATC
NM_014789.3         TGCAATGAGTGTGGGAAAGCCTTTATTCGGAGTTCAAAGCTCATTCAGCATCAGAGGATC
                    ************************************************************

HsCD00329620        CATACTGGGGAGAGGCCTTACGTATGCAATGAGTGTGGGAAGCGCTTCAGCCAGACGTCA
NM_001261843.1      CATACTGGGGAGAGGCCTTACGTATGCAATGAGTGTGGGAAGCGCTTCAGCCAGACGTCA
NM_001082480.2      CATACTGGGGAGAGGCCTTACGTATGCAATGAGTGTGGGAAGCGCTTCAGCCAGACGTCA
NM_014789.3         CATACTGGGGAGAGGCCTTACGTATGCAATGAGTGTGGGAAGCGCTTCAGCCAGACGTCA
                    ************************************************************

HsCD00329620        AACTTCACCCAGCATCAGAGAATTCACACTGGAGAGAAACTCTATGAATGTAACGAGTGT
NM_001261843.1      AACTTCACCCAGCATCAGAGAATTCACACTGGAGAGAAACTCTATGAATGTAACGAGTGT
NM_001082480.2      AACTTCACCCAGCATCAGAGAATTCACACTGGAGAGAAACTCTATGAATGTAACGAGTGT
NM_014789.3         AACTTCACCCAGCATCAGAGAATTCACACTGGAGAGAAACTCTATGAATGTAACGAGTGT
                    ************************************************************

HsCD00329620        GGGAAAGCTTTCTTTCTGAGTTCATACCTTATTCGACACCAGAAAATCCACACTGGAGAG
NM_001261843.1      GGGAAAGCTTTCTTTCTGAGTTCATACCTTATTCGACACCAGAAAATCCACACTGGAGAG
NM_001082480.2      GGGAAAGCTTTCTTTCTGAGTTCATACCTTATTCGACACCAGAAAATCCACACTGGAGAG
NM_014789.3         GGGAAAGCTTTCTTTCTGAGTTCATACCTTATTCGACACCAGAAAATCCACACTGGAGAG
                    ************************************************************

HsCD00329620        AGAGTGTATGAATGTAAGGAATGTGGGAAAGCGTTTCTCCAGAAAGCCCATCTCACTGAG
NM_001261843.1      AGAGTGTATGAATGTAAGGAATGTGGGAAAGCGTTTCTCCAGAAAGCCCATCTCACTGAG
NM_001082480.2      AGAGTGTATGAATGTAAGGAATGTGGGAAAGCGTTTCTCCAGAAAGCCCATCTCACTGAG
NM_014789.3         AGAGTGTATGAATGTAAGGAATGTGGGAAAGCGTTTCTCCAGAAAGCCCATCTCACTGAG
                    ************************************************************

HsCD00329620        CACCAGAAGATCCACTCTGGGGACAGGCCCTTCGAATGTAAAGACTGTGGGAAAGCCTTC
NM_001261843.1      CACCAGAAGATCCACTCTGGGGACAGGCCCTTCGAATGTAAAGACTGTGGGAAAGCCTTC
NM_001082480.2      CACCAGAAGATCCACTCTGGGGACAGGCCCTTCGAATGTAAAGACTGTGGGAAAGCCTTC
NM_014789.3         CACCAGAAGATCCACTCTGGGGACAGGCCCTTCGAATGTAAAGACTGTGGGAAAGCCTTC
                    ************************************************************

HsCD00329620        ATCCAGAGCTCCAAGCTGCTTCTGCACCAGATTATTCACACTGGAGAAAAGCCCTATGTG
NM_001261843.1      ATCCAGAGCTCCAAGCTGCTTCTGCACCAGATTATTCACACTGGAGAAAAGCCCTATGTG
NM_001082480.2      ATCCAGAGCTCCAAGCTGCTTCTGCACCAGATTATTCACACTGGAGAAAAGCCCTATGTG
NM_014789.3         ATCCAGAGCTCCAAGCTGCTTCTGCACCAGATTATTCACACTGGAGAAAAGCCCTATGTG
                    ************************************************************

HsCD00329620        TGCAGTTATTGTGGGAAAGGCTTTATTCAGAGGTCAAACTTCCTTCAACACCAGAAAATT
NM_001261843.1      TGCAGTTATTGTGGGAAAGGCTTTATTCAGAGGTCAAACTTCCTTCAACACCAGAAAATT
NM_001082480.2      TGCAGTTATTGTGGGAAAGGCTTTATTCAGAGGTCAAACTTCCTTCAACACCAGAAAATT
NM_014789.3         TGCAGTTATTGTGGGAAAGGCTTTATTCAGAGGTCAAACTTCCTTCAACACCAGAAAATT
                    ************************************************************

HsCD00329620        CATACTGAAGAGAAGCTCTATGAATGTAGTCAGTATGGGAGAGATTTTAACTCAACTACA
NM_001261843.1      CATACTGAAGAGAAGCTCTATGAATGTAGTCAGTATGGGAGAGATTTTAACTCAACTACA
NM_001082480.2      CATACTGAAGAGAAGCTCTATGAATGTAGTCAGTATGGGAGAGATTTTAACTCAACTACA
NM_014789.3         CATACTGAAGAGAAGCTCTATGAATGTAGTCAGTATGGGAGAGATTTTAACTCAACTACA
                    ************************************************************

HsCD00329620        AACGTTAAAAATAATCAAAGGGTTCACCAAGAGGGACTCTCCTTGAGTAAGGCCCCCATA
NM_001261843.1      AACGTTAAAAATAATCAAAGGGTTCACCAAGAGGGACTCTCCTTGAGTAAGGCCCCCATA
NM_001082480.2      AACGTTAAAAATAATCAAAGGGTTCACCAAGAGGGACTCTCCTTGAGTAAGGCCCCCATA
NM_014789.3         AACGTTAAAAATAATCAAAGGGTTCACCAAGAGGGACTCTCCTTGAGTAAGGCCCCCATA
                    ************************************************************

HsCD00329620        CATTTGGGTGAGAGGTCTGTAGATAAGGGGGAACACACAGGTAACTTATTG
NM_001261843.1      CATTTGGGTGAGAGGTCTGTAGATAAGGGGGAACACACAGGTAACTTATAA
NM_001082480.2      CATTTGGGTGAGAGGTCTGTAGATAAGGGGGAACACACAGGTAACTTATAA
NM_014789.3         CATTTGGGTGAGAGGTCTGTAGATAAGGGGGAACACACAGGTAACTTATAA
                    *************************************************:.

Protein

SMART domain : ZnF_C2H2 : zinc finger
UniProt : O75123
HPRD : 18358

Clone

Cloning Information : 268069
HIP Master Clone ID : 193099
Original Clone ID : FLH268069.01L

Organism


TAX_ID : 9606
Species Specific ID: 9831

Genome

Chromosome : 8
Map Location : 8q24.3
Ensembl : ENSG00000183309

Reagents

Labome : ZNF623-antibody