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CCT4 (Homo sapiens) in pLenti6.2/V5-DEST (V5-tagged lentiviral mammalian expression vector)

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Explanation of Terms


Gene: Gene Symbol:  CCT4
Gene Name:  chaperonin containing TCP1, subunit 4 (delta)
Sequence             
Original Clone ID: FLH268074.01L
Keyword: None
Species: Homo sapiens
Type: cDNA
Vector Name: pLenti6.2/V5-DEST               Format:  FUSION
Source: HIP
Description: None
Comments: None
Mutation/
Discrepancy :
No / No
Publications: None
Authors:
HsCD00329625

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AVAILABLE
No restriction
Special MTA: None

 

Insert sequence: 1620nts         Open reading frame : 1 to 1620
Reference Sequence Alignment

ATGCCCGAGAATGTGGCACCCCGGAGCGGGGCGACTGCCGGGGCTGCCGGCGGCCGCGGG
AAAGGCGCCTATCAGGACCGCGACAAGCCAGCCCAGATCCGCTTCAGCAACATTTCCGCC
GCCAAAGCGGTTGCTGATGCTATTAGAACAAGCCTTGGACCAAAAGGAATGGATAAAATG
ATTCAAGATGGAAAAGGTGATGTAACCATTACAAATGATGGTGCTACCATTCTGAAACAA
ATGCAAGTATTACATCCAGCAGCCAGAATGCTGGTGGAGCTGTCTAAGGCTCAAGATATA
GAAGCAGGAGATGGCACCACATCAGTAGTCATCATTGCTGGCTCCCTCTTAGATTCTTGT
ACCAAGCTTCTTCAGAAAGGGATTCATCCAACCATCATTTCTGAGTCATTCCAGAAGGCC
CTGGAAAAGGGCATTGAAATCTTGACTGACATGTCTCGACCTGTGGAACTGAGTGACAGA
GAAACTTTGTTAAATAGTGCAACCACTTCACTGAACTCAAAGGTGGTTTCTCAGTATTCA
AGTCTGCTTTCTCCAATGAGTGTAAATGCAGTGATGAAAGTGATTGACCCAGCCACAGCC
ACCAGTGTAGATCTTAGAGATATTAAAATAGTTAAGAAGCTTGGTGGGACAATTGATGAC
TGTGAGTTGGTGGAAGGGCTGGTTCTCACCCAAAAAGTGTCAAATTCTGGCATAACCAGA
GTTGAAAAGGCCAAGATTGGGCTTATTCAGTTTTGCTTATCTGCTCCCAAAACAGACATG
GATAATCAAATAGTGGTTTCTGACTATGCCCAGATGGACCGAGTGCTGCGAGAAGAGAGA
GCCTATATTTTAAATTTAGTGAAGCAAATTAAAAAAACAGGATGTAATGTCCTTCTCATA
CAGAAATCTATTCTAAGAGATGCTCTTAGTGATCTTGCATTACACTTTCTGAATAAAATG
AAGATCATGGTGATTAAGGATATTGAAAGAGAAGACATTGAATTCATTTGTAAGACAATT
GGAACCAAGCCAGTTGCTCATATTGACCAATTTACTGCTGACATGCTGGGTTCTGCTGAG
TTAGCTGAGGAGGTCAATTTAAATGGTTCTGGCAAACTGCTCAAGATTACAGGCTGTGCC
AGCCCTGGAAAAACAGTTACAATTGTTGTTCGTGGTTCTAACAAACTGGTGATTGAAGAA
GCTGAGCGCTCCATTCATGATGCCCTATGTGTTATTCGTTGTTTAGTGAAGAAGAGGGCT
CTTATTGCAGGAGGTGGTGCTCCAGAAATAGAGTTGGCCCTACGATTAACTGAATATTCA
CGAACACTGAGTGGTATGGAATCCTACTGCGTTCGTGCTTTTGCAGATGCTATGGAGGTC
ATTCCATCTACACTAGCTGAAAATGCCGGCCTGAATCCCATTTCTACAGTAACAGAACTA
AGAAACCGGCATGCCCAGGGAGAAAAAACTGCAGGCATTAATGTCCGAAAGGGTGGTATT
TCCAACATTTTGGAGGAACTGGTTGTCCAGCCTCTGTTGGTATCAGTCAGTGCTCTGACT
CTTGCAACTGAAACTGTTCGGAGCATTCTGAAAATAGATGATGTGGTAAACACTCGATTG


Coding Sequence Details
Insert Sequence Verified?: N Verification Method: Insert was fully sequenced in parent vector
Reference Sequence Annotations:
End on reference sequence 1720
Start on reference sequence 101
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
5p GTACAAAAAAGCAGGCTCCACC(atg-goi)

3' Linker Sequence:
5p (goi-stop)GACCCAGCTTTCTTGTAC

 

 Recommended Growth Condition:

Distributed in bacterial strain : DH5-alpha T1 phage resistant
Antibiotic Selection: Host Type:  bacterial    Marker:  ampicillin
Host Type:  mammalian    Marker:  blasticidin
Bacterial Selection Condition:  100 ug/mL ampicillin
Growth Condition:  Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:  Commonly used conditions for ampicillin resistant plasmid clones.
Protein Expression Results: None
Recommended expression in: Not Applicable
       DyNA Vector Map

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Vector Name: pLenti6.2/V5-DEST

pLenti6.2V5-DEST in advanced viewed

Synonyms: pLenti6.2/V5-DEST
Sequencing Primer: Forward:  CMV forward
Reverse:  V5 reverse
Description: Lentiviral mammalian expression vector;CMV promoter and C-terminal V5 tag; ampicillin resistance in bacteria; blasticidin resistance in mammalian cells; Gateway recombinational cloning.
Comments: None
Size (bp): 8813
Parent Vector: None
Empty Vector: None
Properties: Gateway, acceptor (destination), lentiviral infection, mammalian expression, recombinational cloning, with tag/fusion/marker
Author Name: Invitrogen
Publications: None
Vector Map:         Vector Sequence:
Sequence:

 



Vector Features:

Type Name Description Start Position End Position
bacterial origin pUC ori pUC origin of replication 7733 8406
death cassette ccdB ccdB death cassette (removed when insert is present) 3767 3976
packaging signal HIV-1 psi HIV-1 psi packaging signal 521 565
poly-A signal SV40 SV40 polyadenylation signal 5639 5770
promoter RSV RSV/5' LTR hybrid promoter 1 410
promoter CMV CMV promoter driving gene of interest 1809 2392
promoter PGK PGK promoter to drive selection marker in mammalian cells 4266 4771
recombination site attR2 attR2 recombinational cloning site 4127 4142
recombination site attR1 attR1 recombinational cloning site 2440 2456
response element HIV-1 RRE HIV-1 Rev response element (RRE) 1075 1308
selectable marker AmpR Ampicillin resistance gene for selection in bacteria 6728 7588
selectable marker BlastR Blasticidin resistance gene in mammalian cells 4849 5249
selectable marker CmR chloramphenicol resistance marker (removed in plasmids with insert) 2673 3329
splice acceptor site 3' splice acceptor two 3' splice acceptors at 1656 and 1684 1656 1656
splice donor site 5' splice donor 5' splice donor 520 520
tag V5 C-terminal V5 epitope tag 4197 4238

 

  • Gene
  • Pathways
  • Protein
  • Clone
  • Organism
  • Genome
  • Reagents

Gene

 

Gene Symbol : CCT4
Symbol Nomenclature : CCT4
SYNONYM : CCT-DELTA; Cctd; SRB
Designation : T-complex protein 1 subunit delta|TCP-1-delta|chaperonin containing t-complex polypeptide 1, delta subunit|stimulator of TAR RNA-binding
Full Nomenclature : chaperonin containing TCP1, subunit 4 (delta)
GENEID : 10575
HGNC : 1617
MIM : 605142
Vega : OTTHUMG00000152166
Target GenBank: BC106934

 

Reference Sequence Alignment

  

HsCD00329625        ATGCCCGAGAATGTGGCACCCCGGAGCGGGGCGACTGCCGGGGCTGCCGGCGGCCGCGGG
NM_006430.3         ATGCCCGAGAATGTGGCACCCCGGAGCGGGGCGACTGCCGGGGCTGCCGGCGGCCGCGGG
NM_001256721.1      ATGCCCGAGAATGTGGCACCCCGGAGCGGGGCGACTGCCGGGGCTGCCGGCGGCCGCGGG
                    ************************************************************

HsCD00329625        AAAGGCGCCTATCAGGACCGCGACAAGCCAGCCCAGATCCGCTTCAGCAACATTTCCGCC
NM_006430.3         AAAGGCGCCTATCAGGACCGCGACAAGCCAGCCCAGATCCGCTTCAGCAACATTTCCGCC
NM_001256721.1      AAAGGCGCCTATCAGGACCGCGACAAGCCAGCCCAGATCCGCTTCAGCAACATTTCCGCC
                    ************************************************************

HsCD00329625        GCCAAAGCGGTTGCTGATGCTATTAGAACAAGCCTTGGACCAAAAGGAATGGATAAAATG
NM_006430.3         GCCAAAGCGGTTGCTGATGCTATTAGAACAAGCCTTGGACCAAAAGGAATGGATAAAATG
NM_001256721.1      GCCAAAGCGGTTGCTGATGCTATTAGAACAAGCCTTGGACCAAAAGGAATG---------
                    ***************************************************         

HsCD00329625        ATTCAAGATGGAAAAGGTGATGTAACCATTACAAATGATGGTGCTACCATTCTGAAACAA
NM_006430.3         ATTCAAGATGGAAAAGGTGATGTAACCATTACAAATGATGGTGCTACCATTCTGAAACAA
NM_001256721.1      ------------------------------------------------------------
                                                                                

HsCD00329625        ATGCAAGTATTACATCCAGCAGCCAGAATGCTGGTGGAGCTGTCTAAGGCTCAAGATATA
NM_006430.3         ATGCAAGTATTACATCCAGCAGCCAGAATGCTGGTGGAGCTGTCTAAGGCTCAAGATATA
NM_001256721.1      ---------------------GATAAAATGCTGGTGGAGCTGTCTAAGGCTCAAGATATA
                                         *. *.**********************************

HsCD00329625        GAAGCAGGAGATGGCACCACATCAGTAGTCATCATTGCTGGCTCCCTCTTAGATTCTTGT
NM_006430.3         GAAGCAGGAGATGGCACCACATCAGTAGTCATCATTGCTGGCTCCCTCTTAGATTCTTGT
NM_001256721.1      GAAGCAGGAGATGGCACCACATCAGTAGTCATCATTGCTGGCTCCCTCTTAGATTCTTGT
                    ************************************************************

HsCD00329625        ACCAAGCTTCTTCAGAAAGGGATTCATCCAACCATCATTTCTGAGTCATTCCAGAAGGCC
NM_006430.3         ACCAAGCTTCTTCAGAAAGGGATTCATCCAACCATCATTTCTGAGTCATTCCAGAAGGCC
NM_001256721.1      ACCAAGCTTCTTCAGAAAGGGATTCATCCAACCATCATTTCTGAGTCATTCCAGAAGGCC
                    ************************************************************

HsCD00329625        CTGGAAAAGGGCATTGAAATCTTGACTGACATGTCTCGACCTGTGGAACTGAGTGACAGA
NM_006430.3         CTGGAAAAGGGCATTGAAATCTTGACTGACATGTCTCGACCTGTGGAACTGAGTGACAGA
NM_001256721.1      CTGGAAAAGGGCATTGAAATCTTGACTGACATGTCTCGACCTGTGGAACTGAGTGACAGA
                    ************************************************************

HsCD00329625        GAAACTTTGTTAAATAGTGCAACCACTTCACTGAACTCAAAGGTGGTTTCTCAGTATTCA
NM_006430.3         GAAACTTTGTTAAATAGTGCAACCACTTCACTGAACTCAAAGGTGGTTTCTCAGTATTCA
NM_001256721.1      GAAACTTTGTTAAATAGTGCAACCACTTCACTGAACTCAAAGGTGGTTTCTCAGTATTCA
                    ************************************************************

HsCD00329625        AGTCTGCTTTCTCCAATGAGTGTAAATGCAGTGATGAAAGTGATTGACCCAGCCACAGCC
NM_006430.3         AGTCTGCTTTCTCCAATGAGTGTAAATGCAGTGATGAAAGTGATTGACCCAGCCACAGCC
NM_001256721.1      AGTCTGCTTTCTCCAATGAGTGTAAATGCAGTGATGAAAGTGATTGACCCAGCCACAGCC
                    ************************************************************

HsCD00329625        ACCAGTGTAGATCTTAGAGATATTAAAATAGTTAAGAAGCTTGGTGGGACAATTGATGAC
NM_006430.3         ACCAGTGTAGATCTTAGAGATATTAAAATAGTTAAGAAGCTTGGTGGGACAATTGATGAC
NM_001256721.1      ACCAGTGTAGATCTTAGAGATATTAAAATAGTTAAGAAGCTTGGTGGGACAATTGATGAC
                    ************************************************************

HsCD00329625        TGTGAGTTGGTGGAAGGGCTGGTTCTCACCCAAAAAGTGTCAAATTCTGGCATAACCAGA
NM_006430.3         TGTGAGTTGGTGGAAGGGCTGGTTCTCACCCAAAAAGTGTCAAATTCTGGCATAACCAGA
NM_001256721.1      TGTGAGTTGGTGGAAGGGCTGGTTCTCACCCAAAAAGTGTCAAATTCTGGCATAACCAGA
                    ************************************************************

HsCD00329625        GTTGAAAAGGCCAAGATTGGGCTTATTCAGTTTTGCTTATCTGCTCCCAAAACAGACATG
NM_006430.3         GTTGAAAAGGCCAAGATTGGGCTTATTCAGTTTTGCTTATCTGCTCCCAAAACAGACATG
NM_001256721.1      GTTGAAAAGGCCAAGATTGGGCTTATTCAGTTTTGCTTATCTGCTCCCAAAACAGACATG
                    ************************************************************

HsCD00329625        GATAATCAAATAGTGGTTTCTGACTATGCCCAGATGGACCGAGTGCTGCGAGAAGAGAGA
NM_006430.3         GATAATCAAATAGTGGTTTCTGACTATGCCCAGATGGACCGAGTGCTGCGAGAAGAGAGA
NM_001256721.1      GATAATCAAATAGTGGTTTCTGACTATGCCCAGATGGACCGAGTGCTGCGAGAAGAGAGA
                    ************************************************************

HsCD00329625        GCCTATATTTTAAATTTAGTGAAGCAAATTAAAAAAACAGGATGTAATGTCCTTCTCATA
NM_006430.3         GCCTATATTTTAAATTTAGTGAAGCAAATTAAAAAAACAGGATGTAATGTCCTTCTCATA
NM_001256721.1      GCCTATATTTTAAATTTAGTGAAGCAAATTAAAAAAACAGGATGTAATGTCCTTCTCATA
                    ************************************************************

HsCD00329625        CAGAAATCTATTCTAAGAGATGCTCTTAGTGATCTTGCATTACACTTTCTGAATAAAATG
NM_006430.3         CAGAAATCTATTCTAAGAGATGCTCTTAGTGATCTTGCATTACACTTTCTGAATAAAATG
NM_001256721.1      CAGAAATCTATTCTAAGAGATGCTCTTAGTGATCTTGCATTACACTTTCTGAATAAAATG
                    ************************************************************

HsCD00329625        AAGATCATGGTGATTAAGGATATTGAAAGAGAAGACATTGAATTCATTTGTAAGACAATT
NM_006430.3         AAGATCATGGTGATTAAGGATATTGAAAGAGAAGACATTGAATTCATTTGTAAGACAATT
NM_001256721.1      AAGATCATGGTGATTAAGGATATTGAAAGAGAAGACATTGAATTCATTTGTAAGACAATT
                    ************************************************************

HsCD00329625        GGAACCAAGCCAGTTGCTCATATTGACCAATTTACTGCTGACATGCTGGGTTCTGCTGAG
NM_006430.3         GGAACCAAGCCAGTTGCTCATATTGACCAATTTACTGCTGACATGCTGGGTTCTGCTGAG
NM_001256721.1      GGAACCAAGCCAGTTGCTCATATTGACCAATTTACTGCTGACATGCTGGGTTCTGCTGAG
                    ************************************************************

HsCD00329625        TTAGCTGAGGAGGTCAATTTAAATGGTTCTGGCAAACTGCTCAAGATTACAGGCTGTGCC
NM_006430.3         TTAGCTGAGGAGGTCAATTTAAATGGTTCTGGCAAACTGCTCAAGATTACAGGCTGTGCC
NM_001256721.1      TTAGCTGAGGAGGTCAATTTAAATGGTTCTGGCAAACTGCTCAAGATTACAGGCTGTGCC
                    ************************************************************

HsCD00329625        AGCCCTGGAAAAACAGTTACAATTGTTGTTCGTGGTTCTAACAAACTGGTGATTGAAGAA
NM_006430.3         AGCCCTGGAAAAACAGTTACAATTGTTGTTCGTGGTTCTAACAAACTGGTGATTGAAGAA
NM_001256721.1      AGCCCTGGAAAAACAGTTACAATTGTTGTTCGTGGTTCTAACAAACTGGTGATTGAAGAA
                    ************************************************************

HsCD00329625        GCTGAGCGCTCCATTCATGATGCCCTATGTGTTATTCGTTGTTTAGTGAAGAAGAGGGCT
NM_006430.3         GCTGAGCGCTCCATTCATGATGCCCTATGTGTTATTCGTTGTTTAGTGAAGAAGAGGGCT
NM_001256721.1      GCTGAGCGCTCCATTCATGATGCCCTATGTGTTATTCGTTGTTTAGTGAAGAAGAGGGCT
                    ************************************************************

HsCD00329625        CTTATTGCAGGAGGTGGTGCTCCAGAAATAGAGTTGGCCCTACGATTAACTGAATATTCA
NM_006430.3         CTTATTGCAGGAGGTGGTGCTCCAGAAATAGAGTTGGCCCTACGATTAACTGAATATTCA
NM_001256721.1      CTTATTGCAGGAGGTGGTGCTCCAGAAATAGAGTTGGCCCTACGATTAACTGAATATTCA
                    ************************************************************

HsCD00329625        CGAACACTGAGTGGTATGGAATCCTACTGCGTTCGTGCTTTTGCAGATGCTATGGAGGTC
NM_006430.3         CGAACACTGAGTGGTATGGAATCCTACTGCGTTCGTGCTTTTGCAGATGCTATGGAGGTC
NM_001256721.1      CGAACACTGAGTGGTATGGAATCCTACTGCGTTCGTGCTTTTGCAGATGCTATGGAGGTC
                    ************************************************************

HsCD00329625        ATTCCATCTACACTAGCTGAAAATGCCGGCCTGAATCCCATTTCTACAGTAACAGAACTA
NM_006430.3         ATTCCATCTACACTAGCTGAAAATGCCGGCCTGAATCCCATTTCTACAGTAACAGAACTA
NM_001256721.1      ATTCCATCTACACTAGCTGAAAATGCCGGCCTGAATCCCATTTCTACAGTAACAGAACTA
                    ************************************************************

HsCD00329625        AGAAACCGGCATGCCCAGGGAGAAAAAACTGCAGGCATTAATGTCCGAAAGGGTGGTATT
NM_006430.3         AGAAACCGGCATGCCCAGGGAGAAAAAACTGCAGGCATTAATGTCCGAAAGGGTGGTATT
NM_001256721.1      AGAAACCGGCATGCCCAGGGAGAAAAAACTGCAGGCATTAATGTCCGAAAGGGTGGTATT
                    ************************************************************

HsCD00329625        TCCAACATTTTGGAGGAACTGGTTGTCCAGCCTCTGTTGGTATCAGTCAGTGCTCTGACT
NM_006430.3         TCCAACATTTTGGAGGAACTGGTTGTCCAGCCTCTGTTGGTATCAGTCAGTGCTCTGACT
NM_001256721.1      TCCAACATTTTGGAGGAACTGGTTGTCCAGCCTCTGTTGGTATCAGTCAGTGCTCTGACT
                    ************************************************************

HsCD00329625        CTTGCAACTGAAACTGTTCGGAGCATTCTGAAAATAGATGATGTGGTAAACACTCGATTG
NM_006430.3         CTTGCAACTGAAACTGTTCGGAGCATTCTGAAAATAGATGATGTGGTAAACACTCGATAA
NM_001256721.1      CTTGCAACTGAAACTGTTCGGAGCATTCTGAAAATAGATGATGTGGTAAACACTCGATAA
                    **********************************************************:.

Pathways

Reactome : Association of TriC/CCT with target proteins during biosynthesis
Reactome : Chaperonin-mediated protein folding
Reactome : Cooperation of Prefoldin and TriC/CCT in actin and tubulin folding
Reactome : Folding of actin by CCT/TriC
Reactome : Formation of tubulin folding intermediates by CCT/TriC
Reactome : Metabolism of proteins
Reactome : Prefoldin mediated transfer of substrate to CCT/TriC
Reactome : Protein folding

Protein

UniProt : P50991
HPRD : 05506

Clone

Cloning Information : 268074
HIP Master Clone ID : 193105
Original Clone ID : FLH268074.01L

Organism


TAX_ID : 9606
Species Specific ID: 10575

Genome

Chromosome : 2
Map Location : 2p15
Ensembl : ENSG00000115484

Reagents

Labome : CCT4-antibody