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ZNF791 (Homo sapiens) in pLenti6.2/V5-DEST (V5-tagged lentiviral mammalian expression vector)

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Explanation of Terms


Gene: Gene Symbol:  ZNF791
Gene Name:  zinc finger protein 791
Sequence             
Original Clone ID: FLH268080.01L
Keyword: None
Species: Homo sapiens
Type: cDNA
Vector Name: pLenti6.2/V5-DEST               Format:  FUSION
Source: HIP
Description: None
Comments: None
Mutation/
Discrepancy :
No / No
Publications: None
Authors:
HsCD00329631

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AVAILABLE
No restriction
Special MTA: None

 

Insert sequence: 1731nts         Open reading frame : 1 to 1731
Reference Sequence Alignment

ATGGACTCAGTGGCTTTTGAGGATGTGTCTGTGAGCTTCAGCCAGGAGGAGTGGGCTCTG
CTGGCTCCTTCACAGAAGAAACTCTACAGAGATGTGATGCAGGAAACATTCAAGAACCTG
GCATCTATAGGGGAAAAATGGGAAGACCCGAATGTTGAAGATCAACACAAAAACCAAGGA
CGAAATCTAAGAAGCCATACGGGAGAGAGACTCTGTGAAGGTAAAGAAGGTAGTCAATGT
GCAGAAAACTTCAGTCCCAATCTCAGTGTGACGAAGAAGACTGCCGGAGTAAAACCATAT
GAGTGTACTATCTGTGGAAAAGCCTTCATGCGTCTCTCATCCCTTACTAGACACATGAGG
TCTCACACTGGATACGAGCTATTTGAGAAGCCATATAAATGTAAGGAGTGTGAGAAAGCC
TTTAGTTATCTCAAATCCTTTCAAAGACATGAAAGGAGTCACACTGGAGAAAAACCCTAT
AAATGTAAACAATGTGGAAAAACCTTCATATATCACCAGCCCTTTCAAAGACATGAGCGG
ACTCACATTGGAGAAAAACCCTATGAATGTAAGCAATGTGGAAAAGCTCTTAGTTGTTCC
AGTTCGCTTCGAGTTCATGAAAGGATTCACACTGGAGAAAAGCCCTATGAATGTAAACAA
TGTGGGAAAGCCTTCAGTTGTTCCAGTTCTATTCGAGTACACGAAAGAACTCACACTGGA
GAGAAACCCTATGCATGTAAGGAATGTGGGAAAGCCTTCATTTCCCACACAAGTGTTCTA
ACACACATGATAACACACAACGGAGATAGACCTTATAAATGCAAAGAATGTGGAAAGGCA
TTCATTTTTCCCAGTTTTTTACGAGTACATGAAAGAATTCACACTGGAGAGAAACCCTAT
AAATGTAAACAATGTGGTAAAGCCTTCAGATGTTCCACCTCCATTCAAATTCATGAAAGA
ATTCATACTGGAGAGAAGCCCTATAAATGTAAAGAATGTGGGAAATCTTTCAGTGCACGC
CCAGCCTTTCGAGTACACGTGAGAGTGCATACTGGAGAGAAACCCTATAAGTGTAAAGAA
TGTGGGAAAGCCTTTAGTAGAATCAGTTACTTTCGAATACATGAAAGGACTCACACTGGA
GAGAAACCCTACGAATGTAAAAAATGTGGGAAAACTTTCAATTATCCTCTAGATTTGAAA
ATCCACAAGAGAAATCACACTGGAGAAAAACCCTATGAGTGTAAGGAATGTGCAAAAACC
TTCATTTCTCTTGAGAACTTTCGAAGACACATGATCACCCACACTGGAGACGGACCTTAT
AAATGTAGGGACTGTGGGAAGGTGTTCATTTTTCCTAGTGCGTTACGAACACATGAAAGA
ACTCACACTGGAGAGAAACCCTATGAATGTAAACAATGTGGAAAAGCCTTTAGTTGTTCT
AGTTACATTCGGATACATAAAAGAACTCACACTGGGGAGAAACCTTATGAATGTAAGGAA
TGCGGGAAGGCCTTTATTTATCCCACAAGCTTTCAAGGACACATGAGAATGCATACTGGA
GAGAAACCCTATAAATGTAAAGAATGTGGGAAGGCCTTTAGTCTTCACAGTTCCTTTCAA
AGACATACAAGAATTCACAATTATGAGAAACCTCTTGAATGTAAGCAATGTGGAAAAGCC
TTCAGTGTGTCCACATCCTTAAAAAAACATATGAGAATGCACAATCGATTG


Coding Sequence Details
Insert Sequence Verified?: N Verification Method: Insert was fully sequenced in parent vector
Reference Sequence Annotations:
End on reference sequence 1827
Start on reference sequence 97
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
5p GTACAAAAAAGCAGGCTCCACC(atg-goi)

3' Linker Sequence:
5p (goi-stop)GACCCAGCTTTCTTGTAC

 

 Recommended Growth Condition:

Distributed in bacterial strain : DH5-alpha T1 phage resistant
Antibiotic Selection: Host Type:  bacterial    Marker:  ampicillin
Host Type:  mammalian    Marker:  blasticidin
Bacterial Selection Condition:  100 ug/mL ampicillin
Growth Condition:  Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:  Commonly used conditions for ampicillin resistant plasmid clones.
Protein Expression Results: None
Recommended expression in: Not Applicable
       DyNA Vector Map

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Vector Name: pLenti6.2/V5-DEST

pLenti6.2V5-DEST in advanced viewed

Synonyms: pLenti6.2/V5-DEST
Sequencing Primer: Forward:  CMV forward
Reverse:  V5 reverse
Description: Lentiviral mammalian expression vector;CMV promoter and C-terminal V5 tag; ampicillin resistance in bacteria; blasticidin resistance in mammalian cells; Gateway recombinational cloning.
Comments: None
Size (bp): 8813
Parent Vector: None
Empty Vector: None
Properties: Gateway, acceptor (destination), lentiviral infection, mammalian expression, recombinational cloning, with tag/fusion/marker
Author Name: Invitrogen
Publications: None
Vector Map:         Vector Sequence:
Sequence:

 



Vector Features:

Type Name Description Start Position End Position
bacterial origin pUC ori pUC origin of replication 7733 8406
death cassette ccdB ccdB death cassette (removed when insert is present) 3767 3976
packaging signal HIV-1 psi HIV-1 psi packaging signal 521 565
poly-A signal SV40 SV40 polyadenylation signal 5639 5770
promoter RSV RSV/5' LTR hybrid promoter 1 410
promoter CMV CMV promoter driving gene of interest 1809 2392
promoter PGK PGK promoter to drive selection marker in mammalian cells 4266 4771
recombination site attR2 attR2 recombinational cloning site 4127 4142
recombination site attR1 attR1 recombinational cloning site 2440 2456
response element HIV-1 RRE HIV-1 Rev response element (RRE) 1075 1308
selectable marker AmpR Ampicillin resistance gene for selection in bacteria 6728 7588
selectable marker BlastR Blasticidin resistance gene in mammalian cells 4849 5249
selectable marker CmR chloramphenicol resistance marker (removed in plasmids with insert) 2673 3329
splice acceptor site 3' splice acceptor two 3' splice acceptors at 1656 and 1684 1656 1656
splice donor site 5' splice donor 5' splice donor 520 520
tag V5 C-terminal V5 epitope tag 4197 4238

 

  • Gene
  • Pathways
  • Protein
  • Clone
  • Organism
  • Genome
  • Reagents

Gene

 

Gene Symbol : ZNF791
Symbol Nomenclature : ZNF791
Full Nomenclature : zinc finger protein 791
GENEID : 163049
HGNC : 26895
Target GenBank: BC106937

 

Reference Sequence Alignment

  

HsCD00329631      ATGGACTCAGTGGCTTTTGAGGATGTGTCTGTGAGCTTCAGCCAGGAGGAGTGGGCTCTG
NM_153358.2       ATGGACTCAGTGGCTTTTGAGGATGTGTCTGTGAGCTTCAGCCAGGAGGAGTGGGCTCTG
                  ************************************************************

HsCD00329631      CTGGCTCCTTCACAGAAGAAACTCTACAGAGATGTGATGCAGGAAACATTCAAGAACCTG
NM_153358.2       CTGGCTCCTTCACAGAAGAAACTCTACAGAGATGTGATGCAGGAAACATTCAAGAACCTG
                  ************************************************************

HsCD00329631      GCATCTATAGGGGAAAAATGGGAAGACCCGAATGTTGAAGATCAACACAAAAACCAAGGA
NM_153358.2       GCATCTATAGGGGAAAAATGGGAAGACCCGAATGTTGAAGATCAACACAAAAACCAAGGA
                  ************************************************************

HsCD00329631      CGAAATCTAAGAAGCCATACGGGAGAGAGACTCTGTGAAGGTAAAGAAGGTAGTCAATGT
NM_153358.2       CGAAATCTAAGAAGCCATACGGGAGAGAGACTCTGTGAAGGTAAAGAAGGTAGTCAATGT
                  ************************************************************

HsCD00329631      GCAGAAAACTTCAGTCCCAATCTCAGTGTGACGAAGAAGACTGCCGGAGTAAAACCATAT
NM_153358.2       GCAGAAAACTTCAGTCCCAATCTCAGTGTGACGAAGAAGACTGCCGGAGTAAAACCATAT
                  ************************************************************

HsCD00329631      GAGTGTACTATCTGTGGAAAAGCCTTCATGCGTCTCTCATCCCTTACTAGACACATGAGG
NM_153358.2       GAGTGTACTATCTGTGGAAAAGCCTTCATGCGTCTCTCATCCCTTACTAGACACATGAGG
                  ************************************************************

HsCD00329631      TCTCACACTGGATACGAGCTATTTGAGAAGCCATATAAATGTAAGGAGTGTGAGAAAGCC
NM_153358.2       TCTCACACTGGATACGAGCTATTTGAGAAGCCATATAAATGTAAGGAGTGTGAGAAAGCC
                  ************************************************************

HsCD00329631      TTTAGTTATCTCAAATCCTTTCAAAGACATGAAAGGAGTCACACTGGAGAAAAACCCTAT
NM_153358.2       TTTAGTTATCTCAAATCCTTTCAAAGACATGAAAGGAGTCACACTGGAGAAAAACCCTAT
                  ************************************************************

HsCD00329631      AAATGTAAACAATGTGGAAAAACCTTCATATATCACCAGCCCTTTCAAAGACATGAGCGG
NM_153358.2       AAATGTAAACAATGTGGAAAAACCTTCATATATCACCAGCCCTTTCAAAGACATGAGCGG
                  ************************************************************

HsCD00329631      ACTCACATTGGAGAAAAACCCTATGAATGTAAGCAATGTGGAAAAGCTCTTAGTTGTTCC
NM_153358.2       ACTCACATTGGAGAAAAACCCTATGAATGTAAGCAATGTGGAAAAGCTCTTAGTTGTTCC
                  ************************************************************

HsCD00329631      AGTTCGCTTCGAGTTCATGAAAGGATTCACACTGGAGAAAAGCCCTATGAATGTAAACAA
NM_153358.2       AGTTCGCTTCGAGTTCATGAAAGGATTCACACTGGAGAAAAGCCCTATGAATGTAAACAA
                  ************************************************************

HsCD00329631      TGTGGGAAAGCCTTCAGTTGTTCCAGTTCTATTCGAGTACACGAAAGAACTCACACTGGA
NM_153358.2       TGTGGGAAAGCCTTCAGTTGTTCCAGTTCTATTCGAGTACACGAAAGAACTCACACTGGA
                  ************************************************************

HsCD00329631      GAGAAACCCTATGCATGTAAGGAATGTGGGAAAGCCTTCATTTCCCACACAAGTGTTCTA
NM_153358.2       GAGAAACCCTATGCATGTAAGGAATGTGGGAAAGCCTTCATTTCCCACACAAGTGTTCTA
                  ************************************************************

HsCD00329631      ACACACATGATAACACACAACGGAGATAGACCTTATAAATGCAAAGAATGTGGAAAGGCA
NM_153358.2       ACACACATGATAACACACAACGGAGATAGACCTTATAAATGCAAAGAATGTGGAAAGGCA
                  ************************************************************

HsCD00329631      TTCATTTTTCCCAGTTTTTTACGAGTACATGAAAGAATTCACACTGGAGAGAAACCCTAT
NM_153358.2       TTCATTTTTCCCAGTTTTTTACGAGTACATGAAAGAATTCACACTGGAGAGAAACCCTAT
                  ************************************************************

HsCD00329631      AAATGTAAACAATGTGGTAAAGCCTTCAGATGTTCCACCTCCATTCAAATTCATGAAAGA
NM_153358.2       AAATGTAAACAATGTGGTAAAGCCTTCAGATGTTCCACCTCCATTCAAATTCATGAAAGA
                  ************************************************************

HsCD00329631      ATTCATACTGGAGAGAAGCCCTATAAATGTAAAGAATGTGGGAAATCTTTCAGTGCACGC
NM_153358.2       ATTCATACTGGAGAGAAGCCCTATAAATGTAAAGAATGTGGGAAATCTTTCAGTGCACGC
                  ************************************************************

HsCD00329631      CCAGCCTTTCGAGTACACGTGAGAGTGCATACTGGAGAGAAACCCTATAAGTGTAAAGAA
NM_153358.2       CCAGCCTTTCGAGTACACGTGAGAGTGCATACTGGAGAGAAACCCTATAAGTGTAAAGAA
                  ************************************************************

HsCD00329631      TGTGGGAAAGCCTTTAGTAGAATCAGTTACTTTCGAATACATGAAAGGACTCACACTGGA
NM_153358.2       TGTGGGAAAGCCTTTAGTAGAATCAGTTACTTTCGAATACATGAAAGGACTCACACTGGA
                  ************************************************************

HsCD00329631      GAGAAACCCTACGAATGTAAAAAATGTGGGAAAACTTTCAATTATCCTCTAGATTTGAAA
NM_153358.2       GAGAAACCCTACGAATGTAAAAAATGTGGGAAAACTTTCAATTATCCTCTAGATTTGAAA
                  ************************************************************

HsCD00329631      ATCCACAAGAGAAATCACACTGGAGAAAAACCCTATGAGTGTAAGGAATGTGCAAAAACC
NM_153358.2       ATCCACAAGAGAAATCACACTGGAGAAAAACCCTATGAGTGTAAGGAATGTGCAAAAACC
                  ************************************************************

HsCD00329631      TTCATTTCTCTTGAGAACTTTCGAAGACACATGATCACCCACACTGGAGACGGACCTTAT
NM_153358.2       TTCATTTCTCTTGAGAACTTTCGAAGACACATGATCACCCACACTGGAGACGGACCTTAT
                  ************************************************************

HsCD00329631      AAATGTAGGGACTGTGGGAAGGTGTTCATTTTTCCTAGTGCGTTACGAACACATGAAAGA
NM_153358.2       AAATGTAGGGACTGTGGGAAGGTGTTCATTTTTCCTAGTGCGTTACGAACACATGAAAGA
                  ************************************************************

HsCD00329631      ACTCACACTGGAGAGAAACCCTATGAATGTAAACAATGTGGAAAAGCCTTTAGTTGTTCT
NM_153358.2       ACTCACACTGGAGAGAAACCCTATGAATGTAAACAATGTGGAAAAGCCTTTAGTTGTTCT
                  ************************************************************

HsCD00329631      AGTTACATTCGGATACATAAAAGAACTCACACTGGGGAGAAACCTTATGAATGTAAGGAA
NM_153358.2       AGTTACATTCGGATACATAAAAGAACTCACACTGGGGAGAAACCTTATGAATGTAAGGAA
                  ************************************************************

HsCD00329631      TGCGGGAAGGCCTTTATTTATCCCACAAGCTTTCAAGGACACATGAGAATGCATACTGGA
NM_153358.2       TGCGGGAAGGCCTTTATTTATCCCACAAGCTTTCAAGGACACATGAGAATGCATACTGGA
                  ************************************************************

HsCD00329631      GAGAAACCCTATAAATGTAAAGAATGTGGGAAGGCCTTTAGTCTTCACAGTTCCTTTCAA
NM_153358.2       GAGAAACCCTATAAATGTAAAGAATGTGGGAAGGCCTTTAGTCTTCACAGTTCCTTTCAA
                  ************************************************************

HsCD00329631      AGACATACAAGAATTCACAATTATGAGAAACCTCTTGAATGTAAGCAATGTGGAAAAGCC
NM_153358.2       AGACATACAAGAATTCACAATTATGAGAAACCTCTTGAATGTAAGCAATGTGGAAAAGCC
                  ************************************************************

HsCD00329631      TTCAGTGTGTCCACATCCTTAAAAAAACATATGAGAATGCACAATCGATTG
NM_153358.2       TTCAGTGTGTCCACATCCTTAAAAAAACATATGAGAATGCACAATCGATAG
                  *************************************************:*

Pathways

Reactome : Gene Expression
Reactome : Generic Transcription Pathway

Protein

SMART domain : KRAB : krueppel associated box
SMART domain : ZnF_C2H2 : zinc finger
UniProt : Q3KP31
HPRD : 08295

Clone

Cloning Information : 268080
HIP Master Clone ID : 193111
Original Clone ID : FLH268080.01L

Organism


TAX_ID : 9606
Species Specific ID: 163049

Genome

Chromosome : 19
Map Location : 19p13.2
Ensembl : ENSG00000173875

Reagents

Labome : ZNF791-antibody