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RNF41 (Homo sapiens) in pLenti6.2/V5-DEST (V5-tagged lentiviral mammalian expression vector)

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Explanation of Terms


Gene: Gene Symbol:  RNF41
Gene Name:  ring finger protein 41
Sequence             
Original Clone ID: FLH268081.01L
Keyword: None
Species: Homo sapiens
Type: cDNA
Vector Name: pLenti6.2/V5-DEST               Format:  FUSION
Source: HIP
Description: None
Comments: None
Mutation/
Discrepancy :
No / No
Publications: None
Authors:
HsCD00329632

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AVAILABLE
No restriction
Special MTA: None

 

Insert sequence: 954nts         Open reading frame : 1 to 954
Reference Sequence Alignment

ATGGGGTATGATGTAACCCGTTTCCAGGGGGATGTTGACGAAGATCTTATCTGCCCTATT
TGCAGTGGAGTCTTGGAGGAGCCAGTACAGGCACCTCATTGTGAACATGCTTTCTGCAAC
GCCTGCATCACCCAGTGGTTCTCTCAGCAACAGACATGTCCAGTGGACCGTAGTGTTGTG
ACGGTCGCCCATCTGCGCCCAGTACCTCGGATCATGCGGAACATGTTGTCAAAGCTGCAG
ATTGCCTGTGACAACGCTGTGTTCGGCTGTAGTGCCGTTGTCCGGCTTGACAACCTCATG
TCTCACCTCAGCGACTGTGAGCACAACCCGAAGCGGCCTGTGACCTGTGAACAGGGCTGT
GGCCTGGAGATGCCCAAAGATGAGCTGCCCAACCATAACTGCATTAAGCACCTGCGCTCA
GTGGTACAGCAGCAGCAGACACGCATCGCAGAGCTGGAGAAGACGTCAGCTGAACACAAA
CACCAGCTGGCGGAGCAGAAGCGAGACATCCAGCTGCTAAAGGCATACATGCGTGCAATC
CGCAGTGTCAACCCCAACCTTCAGAACCTGGAGGAGACAATTGAATACAACGAGATCCTA
GAGTGGGTGAACTCCCTTCAGCCAGCAAGAGTGACCCGCTGGGGAGGGATGATCTCGACT
CCTGATGCTGTGCTCCAGGCTGTAATCAAGCGCTCCCTGGTGGAGAGTGGCTGTCCTGCT
TCTATTGTCAACGAGCTGATTGAAAATGCCCACGAGCGTAGCTGGCCCCAGGGTCTGGCC
ACACTAGAGACTAGACAGATGAACCGACGCTACTATGAGAACTACGTGGCCAAGCGCATC
CCTGGCAAGCAGGCTGTTGTCGTGATGGCCTGTGAGAACCAGCACATGGGGGATGACATG
GTGCAAGAGCCAGGCCTTGTCATGATATTTGCGCATGGCGTGGAAGAGATATTG


Coding Sequence Details
Insert Sequence Verified?: N Verification Method: Insert was fully sequenced in parent vector
Reference Sequence Annotations:
End on reference sequence 1182
Start on reference sequence 229
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
5p GTACAAAAAAGCAGGCTCCACC(atg-goi)

3' Linker Sequence:
5p (goi-stop)GACCCAGCTTTCTTGTAC

 

 Recommended Growth Condition:

Distributed in bacterial strain : DH5-alpha T1 phage resistant
Antibiotic Selection: Host Type:  bacterial    Marker:  ampicillin
Host Type:  mammalian    Marker:  blasticidin
Bacterial Selection Condition:  100 ug/mL ampicillin
Growth Condition:  Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:  Commonly used conditions for ampicillin resistant plasmid clones.
Protein Expression Results: None
Recommended expression in: Not Applicable
       DyNA Vector Map

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Vector Name: pLenti6.2/V5-DEST

pLenti6.2V5-DEST in advanced viewed

Synonyms: pLenti6.2/V5-DEST
Sequencing Primer: Forward:  CMV forward
Reverse:  V5 reverse
Description: Lentiviral mammalian expression vector;CMV promoter and C-terminal V5 tag; ampicillin resistance in bacteria; blasticidin resistance in mammalian cells; Gateway recombinational cloning.
Comments: None
Size (bp): 8813
Parent Vector: None
Empty Vector: None
Properties: Gateway, acceptor (destination), lentiviral infection, mammalian expression, recombinational cloning, with tag/fusion/marker
Author Name: Invitrogen
Publications: None
Vector Map:         Vector Sequence:
Sequence:

 



Vector Features:

Type Name Description Start Position End Position
bacterial origin pUC ori pUC origin of replication 7733 8406
death cassette ccdB ccdB death cassette (removed when insert is present) 3767 3976
packaging signal HIV-1 psi HIV-1 psi packaging signal 521 565
poly-A signal SV40 SV40 polyadenylation signal 5639 5770
promoter RSV RSV/5' LTR hybrid promoter 1 410
promoter CMV CMV promoter driving gene of interest 1809 2392
promoter PGK PGK promoter to drive selection marker in mammalian cells 4266 4771
recombination site attR2 attR2 recombinational cloning site 4127 4142
recombination site attR1 attR1 recombinational cloning site 2440 2456
response element HIV-1 RRE HIV-1 Rev response element (RRE) 1075 1308
selectable marker AmpR Ampicillin resistance gene for selection in bacteria 6728 7588
selectable marker BlastR Blasticidin resistance gene in mammalian cells 4849 5249
selectable marker CmR chloramphenicol resistance marker (removed in plasmids with insert) 2673 3329
splice acceptor site 3' splice acceptor two 3' splice acceptors at 1656 and 1684 1656 1656
splice donor site 5' splice donor 5' splice donor 520 520
tag V5 C-terminal V5 epitope tag 4197 4238

 

  • Gene
  • Pathways
  • Protein
  • Clone
  • Organism
  • Genome
  • Reagents

Gene

 

Gene Symbol : RNF41
Symbol Nomenclature : RNF41
SYNONYM : FLRF; NRDP1
Designation : E3 ubiquitin-protein ligase NRDP1|fetal liver ring finger|neuregulin receptor degradation protein-1
Full Nomenclature : ring finger protein 41
GENEID : 10193
Locus Tag : SBBI03
HGNC : 18401
Target GenBank: BC032637

 

Reference Sequence Alignment

  

HsCD00329632        ATGGGGTATGATGTAACCCGTTTCCAGGGGGATGTTGACGAAGATCTTATCTGCCCTATT
NM_001242826.1      ATGGGGTATGATGTAACCCGTTTCCAGGGGGATGTTGACGAAGATCTTATCTGCCCTATT
NM_005785.3         ATGGGGTATGATGTAACCCGTTTCCAGGGGGATGTTGACGAAGATCTTATCTGCCCTATT
NM_194359.2         ATGGGGTATGATGTAACCCGTTTCCAGGGGGATGTTGACGAAGATCTTATCTGCCCTATT
                    ************************************************************

HsCD00329632        TGCAGTGGAGTCTTGGAGGAGCCAGTACAGGCACCTCATTGTGAACATGCTTTCTGCAAC
NM_001242826.1      TGCAGTGGAGTCTTGGAGGAGCCAGTACAGGCACCTCATTGTGAACATGCTTTCTGCAAC
NM_005785.3         TGCAGTGGAGTCTTGGAGGAGCCAGTACAGGCACCTCATTGTGAACATGCTTTCTGCAAC
NM_194359.2         TGCAGTGGAGTCTTGGAGGAGCCAGTACAGGCACCTCATTGTGAACATGCTTTCTGCAAC
                    ************************************************************

HsCD00329632        GCCTGCATCACCCAGTGGTTCTCTCAGCAACAGACATGTCCAGTGGACCGTAGTGTTGTG
NM_001242826.1      GCCTGCATCACCCAGTGGTTCTCTCAGCAACAGACATGTCCAGTGGACCGTAGTGTTGTG
NM_005785.3         GCCTGCATCACCCAGTGGTTCTCTCAGCAACAGACATGTCCAGTGGACCGTAGTGTTGTG
NM_194359.2         GCCTGCATCACCCAGTGGTTCTCTCAGCAACAGACATGTCCAGTGGACCGTAGTGTTGTG
                    ************************************************************

HsCD00329632        ACGGTCGCCCATCTGCGCCCAGTACCTCGGATCATGCGGAACATGTTGTCAAAGCTGCAG
NM_001242826.1      ACGGTCGCCCATCTGCGCCCAGTACCTCGGATCATGCGGAACATGTTGTCAAAGCTGCAG
NM_005785.3         ACGGTCGCCCATCTGCGCCCAGTACCTCGGATCATGCGGAACATGTTGTCAAAGCTGCAG
NM_194359.2         ACGGTCGCCCATCTGCGCCCAGTACCTCGGATCATGCGGAACATGTTGTCAAAGCTGCAG
                    ************************************************************

HsCD00329632        ATTGCCTGTGACAACGCTGTGTTCGGCTGTAGTGCCGTTGTCCGGCTTGACAACCTCATG
NM_001242826.1      ATTGCCTGTGACAACGCTGTGTTCGGCTGTAGTGCCGTTGTCCGGCTTGACAACCTCATG
NM_005785.3         ATTGCCTGTGACAACGCTGTGTTCGGCTGTAGTGCCGTTGTCCGGCTTGACAACCTCATG
NM_194359.2         ATTGCCTGTGACAACGCTGTGTTCGGCTGTAGTGCCGTTGTCCGGCTTGACAACCTCATG
                    ************************************************************

HsCD00329632        TCTCACCTCAGCGACTGTGAGCACAACCCGAAGCGGCCTGTGACCTGTGAACAGGGCTGT
NM_001242826.1      TCTCACCTCAGCGACTGTGAGCACAACCCGAAGCGGCCTGTGACCTGTGAACAGGGCTGT
NM_005785.3         TCTCACCTCAGCGACTGTGAGCACAACCCGAAGCGGCCTGTGACCTGTGAACAGGGCTGT
NM_194359.2         TCTCACCTCAGCGACTGTGAGCACAACCCGAAGCGGCCTGTGACCTGTGAACAGGGCTGT
                    ************************************************************

HsCD00329632        GGCCTGGAGATGCCCAAAGATGAGCTGCCCAACCATAACTGCATTAAGCACCTGCGCTCA
NM_001242826.1      GGCCTGGAGATGCCCAAAGATGAGCTGCCCAACCATAACTGCATTAAGCACCTGCGCTCA
NM_005785.3         GGCCTGGAGATGCCCAAAGATGAGCTGCCCAACCATAACTGCATTAAGCACCTGCGCTCA
NM_194359.2         GGCCTGGAGATGCCCAAAGATGAGCTGCCCAACCATAACTGCATTAAGCACCTGCGCTCA
                    ************************************************************

HsCD00329632        GTGGTACAGCAGCAGCAGACACGCATCGCAGAGCTGGAGAAGACGTCAGCTGAACACAAA
NM_001242826.1      GTGGTACAGCAGCAGCAGACACGCATCGCAGAGCTGGAGAAGACGTCAGCTGAACACAAA
NM_005785.3         GTGGTACAGCAGCAGCAGACACGCATCGCAGAGCTGGAGAAGACGTCAGCTGAACACAAA
NM_194359.2         GTGGTACAGCAGCAGCAGACACGCATCGCAGAGCTGGAGAAGACGTCAGCTGAACACAAA
                    ************************************************************

HsCD00329632        CACCAGCTGGCGGAGCAGAAGCGAGACATCCAGCTGCTAAAGGCATACATGCGTGCAATC
NM_001242826.1      CACCAGCTGGCGGAGCAGAAGCGAGACATCCAGCTGCTAAAGGCATACATGCGTGCAATC
NM_005785.3         CACCAGCTGGCGGAGCAGAAGCGAGACATCCAGCTGCTAAAGGCATACATGCGTGCAATC
NM_194359.2         CACCAGCTGGCGGAGCAGAAGCGAGACATCCAGCTGCTAAAGGCATACATGCGTGCAATC
                    ************************************************************

HsCD00329632        CGCAGTGTCAACCCCAACCTTCAGAACCTGGAGGAGACAATTGAATACAACGAGATCCTA
NM_001242826.1      CGCAGTGTCAACCCCAACCTTCAGAACCTGGAGGAGACAATTGAATACAACGAGATCCTA
NM_005785.3         CGCAGTGTCAACCCCAACCTTCAGAACCTGGAGGAGACAATTGAATACAACGAGATCCTA
NM_194359.2         CGCAGTGTCAACCCCAACCTTCAGAACCTGGAGGAGACAATTGAATACAACGAGATCCTA
                    ************************************************************

HsCD00329632        GAGTGGGTGAACTCCCTTCAGCCAGCAAGAGTGACCCGCTGGGGAGGGATGATCTCGACT
NM_001242826.1      GAGTGGGTGAACTCCCTTCAGCCAGCAAGAGTGACCCGCTGGGGAGGGATGATCTCGACT
NM_005785.3         GAGTGGGTGAACTCCCTTCAGCCAGCAAGAGTGACCCGCTGGGGAGGGATGATCTCGACT
NM_194359.2         GAGTGGGTGAACTCCCTTCAGCCAGCAAGAGTGACCCGCTGGGGAGGGATGATCTCGACT
                    ************************************************************

HsCD00329632        CCTGATGCTGTGCTCCAGGCTGTAATCAAGCGCTCCCTGGTGGAGAGTGGCTGTCCTGCT
NM_001242826.1      CCTGATGCTGTGCTCCAGGCTGTAATCAAGCGCTCCCTGGTGGAGAGTGGCTGTCCTGCT
NM_005785.3         CCTGATGCTGTGCTCCAGGCTGTAATCAAGCGCTCCCTGGTGGAGAGTGGCTGTCCTGCT
NM_194359.2         CCTGATGCTGTGCTCCAGGCTGTAATCAAGCGCTCCCTGGTGGAGAGTGGCTGTCCTGCT
                    ************************************************************

HsCD00329632        TCTATTGTCAACGAGCTGATTGAAAATGCCCACGAGCGTAGCTGGCCCCAGGGTCTGGCC
NM_001242826.1      TCTATTGTCAACGAGCTGATTGAAAATGCCCACGAGCGTAGCTGGCCCCAGGGTCTGGCC
NM_005785.3         TCTATTGTCAACGAGCTGATTGAAAATGCCCACGAGCGTAGCTGGCCCCAGGGTCTGGCC
NM_194359.2         TCTATTGTCAACGAGCTGATTGAAAATGCCCACGAGCGTAGCTGGCCCCAGGGTCTGGCC
                    ************************************************************

HsCD00329632        ACACTAGAGACTAGACAGATGAACCGACGCTACTATGAGAACTACGTGGCCAAGCGCATC
NM_001242826.1      ACACTAGAGACTAGACAGATGAACCGACGCTACTATGAGAACTACGTGGCCAAGCGCATC
NM_005785.3         ACACTAGAGACTAGACAGATGAACCGACGCTACTATGAGAACTACGTGGCCAAGCGCATC
NM_194359.2         ACACTAGAGACTAGACAGATGAACCGACGCTACTATGAGAACTACGTGGCCAAGCGCATC
                    ************************************************************

HsCD00329632        CCTGGCAAGCAGGCTGTTGTCGTGATGGCCTGTGAGAACCAGCACATGGGGGATGACATG
NM_001242826.1      CCTGGCAAGCAGGCTGTTGTCGTGATGGCCTGTGAGAACCAGCACATGGGGGATGACATG
NM_005785.3         CCTGGCAAGCAGGCTGTTGTCGTGATGGCCTGTGAGAACCAGCACATGGGGGATGACATG
NM_194359.2         CCTGGCAAGCAGGCTGTTGTCGTGATGGCCTGTGAGAACCAGCACATGGGGGATGACATG
                    ************************************************************

HsCD00329632        GTGCAAGAGCCAGGCCTTGTCATGATATTTGCGCATGGCGTGGAAGAGATATTG
NM_001242826.1      GTGCAAGAGCCAGGCCTTGTCATGATATTTGCGCATGGCGTGGAAGAGATATAA
NM_005785.3         GTGCAAGAGCCAGGCCTTGTCATGATATTTGCGCATGGCGTGGAAGAGATATAA
NM_194359.2         GTGCAAGAGCCAGGCCTTGTCATGATATTTGCGCATGGCGTGGAAGAGATATAA
                    ****************************************************:.

Pathways

NCI : ErbB2/ErbB3 signaling events
Reactome : Adaptive Immune System
Reactome : Antigen processing: Ubiquitination & Proteasome degradation
Reactome : Class I MHC mediated antigen processing & presentation
Reactome : Downregulation of ERBB2:ERBB3 signaling
Reactome : Immune System
Reactome : Signal Transduction
Reactome : Signaling by ERBB2
WikiPathway : Regulation of toll-like receptor signaling pathway

Protein

SMART domain : Ubox : Modified RING finger domain
SMART domain : RING : Ring finger
UniProt : A6NFW0
UniProt : Q9H4P4
HPRD : 11520

Clone

Cloning Information : 268081
HIP Master Clone ID : 193112
Original Clone ID : FLH268081.01L

Organism


TAX_ID : 9606
Species Specific ID: 10193

Genome

Chromosome : 12
Map Location : 12q13.13

Reagents

Labome : RNF41-antibody