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ARID3A (Homo sapiens) in pLenti6.2/V5-DEST (V5-tagged lentiviral mammalian expression vector)

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Explanation of Terms


Gene: Gene Symbol:  ARID3A
Gene Name:  AT rich interactive domain 3A (BRIGHT-like)
Sequence             
Original Clone ID: FLH268084.01L
Keyword: None
Species: Homo sapiens
Type: cDNA
Vector Name: pLenti6.2/V5-DEST               Format:  FUSION
Source: HIP
Description: None
Comments: None
Mutation/
Discrepancy :
No / No
Publications: None
Authors:
HsCD00329635

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AVAILABLE
No restriction
Special MTA: None

 

Insert sequence: 1782nts         Open reading frame : 1 to 1782
Reference Sequence Alignment

ATGAAACTACAGGCCGTGATGGAGACGCTGTTGCAGCGGCAGCAGCGGGCGCGCCAGGAG
CTGGAGGCCCGGCAGCAGCTGCCCCCCGATCCCCCTGCTGCACCCCACGGCCGGGCCCGG
GCTGCCCCCGACGAGGACAGAGAGCCCGAAAGTGCCCGGATGCAGCGGGCTCAGATGGCC
GCACTGGCAGCCATGCGGGCTGCAGCTGCGGGCCTGGGACACCCGGCCAGCCCCGGCGGC
TCTGAGGATGGGCCCCCAGGCTCGGAGGAGGAGGACGCGGCCCGGGAGGGGACACCGGGC
TCACCCGGGCGAGGCAGAGAAGGGCCAGGAGAGGAGCACTTTGAGGACATGGCCTCCGAC
GAGGACATGAAGCCCAAATGGGAGGAGGAGGAGATGGAGGAAGACCTCGGGGAGGATGAG
GAGGAGGAGGAGGAGGATTACGAGGATGAGGAGGAGGAGGAGGACGAGGAGGGGCTGGGC
CCCCCAGGCCCTGCCAGCTTGGGCACCACGGCACTGTTCCCCCGAAAGGCCCAGCCACCC
CAGGCCTTCCGCGGCGATGGCGTTCCCAGGGTGCTGGGGGGCCAGGAGCGGCCGGGGCCT
GGCCCTGCCCACCCCGGAGGGGCCGCCCACGTAGCCCCGCAGCTGCAGCCGCCTGACCAC
GGCGACTGGACTTACGAGGAGCAGTTTAAGCAGCTCTACGAACTCGACGGGGACCCCAAG
AGGAAGGAATTCCTGGATGACTTGTTCAGCTTCATGCAGAAGCGAGGGACACCTGTGAAC
CGCATCCCCATCATGGCCAAACAGGTCCTTGACCTGTTCATGCTGTACGTGCTGGTGACG
GAGAAGGGCGGCCTCGTGGAGGTCATCAACAAGAAGCTGTGGCGTGAGATCACCAAGGGC
CTCAACCTGCCCACGTCCATCACCAGTGCAGCCTTCACCCTGCGGACCCAATACATGGAG
TACCTGTACCCCTACGAGTGTGAGAAGCGGGGCCTCAGTAACCCCAATGAGCTCCAGGCA
GCCATAGACAGCAACCGACGGGAGGGCCGGCGCCAGAGCTTTGGTGGCTCCCTCTTTGCC
TACTCGCCAGGCGGGGCACACGGCATGCTCTCCTCACCCAAGCTACCCGTGTCCTCCCTG
GGCCTGGCCGCAAGCACCAACGGCAGCTCCATCACCCCCGCCCCTAAGATCAAGAAAGAG
GAGGACTCAGCCATCCCCATCACAGTCCCTGGCCGCCTGCCTGTGTCCCTGGCGGGCCAC
CCTGTGGTGGCAGCCCAGGCAGCAGCTGTGCAAGCAGCAGCCGCCCAAGCAGCTGTGGCT
GCACAGGCAGCTGCCCTGGAACAGCTGCGGGAGAAGCTGGAGTCTGCGGAGCCTCCGGAG
AAGAAGATGGCCCTGGTGGCCGATGAGCAGCAACGGCTGATGCAACGTGCACTCCAGCAG
AACTTCCTGGCCATGGCGGCCCAGCTGCCCATGAGCATTCGGATCAACAGCCAAGCCTCC
GAAAGCCGCCAGGACTCTGCTGTGAACCTGACGGGCACCAACGGCAGCAACAGCATCAGC
ATGTCGGTGGAGATCAACGGCATCATGTACACAGGAGTTCTGTTTGCTCAGCCGCCGGCC
CCCACGCCAACCTCTGCTCCCAACAAAGGAGGCGGCGGCGGCGGCAGCAGCAGCAGCAAC
GCAGGCGGCCGGGGAGGAAACACCGGAACCAGCGGCGGCCAGGCTGGGCCAGCGGGGCTG
TCCACACCCTCCACATCTACCTCAAATAACTCGTTGCCTTTG


Coding Sequence Details
Insert Sequence Verified?: N Verification Method: Insert was fully sequenced in parent vector
Reference Sequence Annotations:
End on reference sequence 2072
Start on reference sequence 291
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
5p GTACAAAAAAGCAGGCTCCACC(atg-goi)

3' Linker Sequence:
5p (goi-stop)GACCCAGCTTTCTTGTAC

 

 Recommended Growth Condition:

Distributed in bacterial strain : DH5-alpha T1 phage resistant
Antibiotic Selection: Host Type:  bacterial    Marker:  ampicillin
Host Type:  mammalian    Marker:  blasticidin
Bacterial Selection Condition:  100 ug/mL ampicillin
Growth Condition:  Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:  Commonly used conditions for ampicillin resistant plasmid clones.
Protein Expression Results: None
Recommended expression in: Not Applicable
       DyNA Vector Map

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Vector Name: pLenti6.2/V5-DEST

pLenti6.2V5-DEST in advanced viewed

Synonyms: pLenti6.2/V5-DEST
Sequencing Primer: Forward:  CMV forward
Reverse:  V5 reverse
Description: Lentiviral mammalian expression vector;CMV promoter and C-terminal V5 tag; ampicillin resistance in bacteria; blasticidin resistance in mammalian cells; Gateway recombinational cloning.
Comments: None
Size (bp): 8813
Parent Vector: None
Empty Vector: None
Properties: Gateway, acceptor (destination), lentiviral infection, mammalian expression, recombinational cloning, with tag/fusion/marker
Author Name: Invitrogen
Publications: None
Vector Map:         Vector Sequence:
Sequence:

 



Vector Features:

Type Name Description Start Position End Position
bacterial origin pUC ori pUC origin of replication 7733 8406
death cassette ccdB ccdB death cassette (removed when insert is present) 3767 3976
packaging signal HIV-1 psi HIV-1 psi packaging signal 521 565
poly-A signal SV40 SV40 polyadenylation signal 5639 5770
promoter RSV RSV/5' LTR hybrid promoter 1 410
promoter CMV CMV promoter driving gene of interest 1809 2392
promoter PGK PGK promoter to drive selection marker in mammalian cells 4266 4771
recombination site attR2 attR2 recombinational cloning site 4127 4142
recombination site attR1 attR1 recombinational cloning site 2440 2456
response element HIV-1 RRE HIV-1 Rev response element (RRE) 1075 1308
selectable marker AmpR Ampicillin resistance gene for selection in bacteria 6728 7588
selectable marker BlastR Blasticidin resistance gene in mammalian cells 4849 5249
selectable marker CmR chloramphenicol resistance marker (removed in plasmids with insert) 2673 3329
splice acceptor site 3' splice acceptor two 3' splice acceptors at 1656 and 1684 1656 1656
splice donor site 5' splice donor 5' splice donor 520 520
tag V5 C-terminal V5 epitope tag 4197 4238

 

  • Gene
  • Pathways
  • Protein
  • Clone
  • Organism
  • Genome
  • Reagents

Gene

 

Gene Symbol : ARID3A
Symbol Nomenclature : ARID3A
SYNONYM : BRIGHT; DRIL1; DRIL3; E2FBP1
Designation : ARID domain-containing 3A|ARID domain-containing protein 3A|AT rich interactive domain 3A (BRIGHT- like) protein|AT-rich interactive domain-containing protein 3A|B-cell regulator of IgH transcription|E2F-binding protein 1|dead ringer-like 1|dead ringer-like protein 1
Full Nomenclature : AT rich interactive domain 3A (BRIGHT-like)
GENEID : 1820
HGNC : 3031
MIM : 603265
Target GenBank: BC060828

 

Reference Sequence Alignment

  

HsCD00329635        ATGAAACTACAGGCCGTGATGGAGACGCTGTTGCAGCGGCAGCAGCGGGCGCGCCAGGAG
XM_005259512.1      ATGAAACTACAGGCCGTGATGGAGACGCTGTTGCAGCGGCAGCAGCGGGCGCGCCAGGAG
NM_005224.2         ATGAAACTACAGGCCGTGATGGAGACGCTGTTGCAGCGGCAGCAGCGGGCGCGCCAGGAG
                    ************************************************************

HsCD00329635        CTGGAGGCCCGGCAGCAGCTGCCCCCCGATCCCCCTGCTGCACCCCACGGCCGGGCCCGG
XM_005259512.1      CTGGAGGCCCGGCAGCAGCTGCCCCCCGATCCCCCTGCTGCACCCCCCGGCCGGGCCCGG
NM_005224.2         CTGGAGGCCCGGCAGCAGCTGCCCCCCGATCCCCCTGCTGCACCCCCCGGCCGGGCCCGG
                    **********************************************.*************

HsCD00329635        GCTGCCCCCGACGAGGACAGAGAGCCCGAAAGTGCCCGGATGCAGCGGGCTCAGATGGCC
XM_005259512.1      GCTGCCCCCGACGAGGACAGAGAGCCCGAGAGTGCCCGGATGCAGCGGGCTCAGATGGCC
NM_005224.2         GCTGCCCCCGACGAGGACAGAGAGCCCGAGAGTGCCCGGATGCAGCGGGCTCAGATGGCC
                    *****************************.******************************

HsCD00329635        GCACTGGCAGCCATGCGGGCTGCAGCTGCGGGCCTGGGACACCCGGCCAGCCCCGGCGGC
XM_005259512.1      GCACTGGCAGCCATGCGGGCTGCAGCTGCGGGCCTGGGACACCCAGCCAGCCCCGGCGGC
NM_005224.2         GCACTGGCAGCCATGCGGGCTGCAGCTGCGGGCCTGGGACACCCAGCCAGCCCCGGCGGC
                    ********************************************.***************

HsCD00329635        TCTGAGGATGGGCCCCCAGGCTCGGAGGAGGAGGACGCGGCCCGGGAGGGGACACCGGGC
XM_005259512.1      TCTGAGGATGGGCCCCCAGGCTCGGAGGAGGAGGACGCGGCCCGGGAGGGGACACCGGGC
NM_005224.2         TCTGAGGATGGGCCCCCAGGCTCGGAGGAGGAGGACGCGGCCCGGGAGGGGACACCGGGC
                    ************************************************************

HsCD00329635        TCACCCGGGCGAGGCAGAGAAGGGCCAGGAGAGGAGCACTTTGAGGACATGGCCTCCGAC
XM_005259512.1      TCACCCGGGCGAGGCAGAGAAGGGCCAGGAGAGGAGCACTTTGAGGACATGGCCTCCGAC
NM_005224.2         TCACCCGGGCGAGGCAGAGAAGGGCCAGGAGAGGAGCACTTTGAGGACATGGCCTCCGAC
                    ************************************************************

HsCD00329635        GAGGACATGAAGCCCAAATGGGAGGAGGAGGAGATGGAGGAAGACCTCGGGGAGGATGAG
XM_005259512.1      GAGGACATGAAGCCCAAATGGGAGGAGGAGGAGATGGAGGAAGACCTCGGGGAGGATGAG
NM_005224.2         GAGGACATGAAGCCCAAATGGGAGGAGGAGGAGATGGAGGAAGACCTCGGGGAGGATGAG
                    ************************************************************

HsCD00329635        GAGGAGGAGGAGGAGGATTACGAGGATGAGGAGGAGGAGGAGGACGAGGAGGGGCTGGGC
XM_005259512.1      GAGGAGGAGGAGGAGGATTACGAGGATGAGGAGGAGGAGGAGGACGAGGAGGGGCTGGGC
NM_005224.2         GAGGAGGAGGAGGAGGATTACGAGGATGAGGAGGAGGAGGAGGACGAGGAGGGGCTGGGC
                    ************************************************************

HsCD00329635        CCCCCAGGCCCTGCCAGCTTGGGCACCACGGCACTGTTCCCCCGAAAGGCCCAGCCACCC
XM_005259512.1      CCCCCAGGCCCTGCCAGCTTGGGCACCACGGCACTGTTCCCCCGAAAGGCCCAGCCACCC
NM_005224.2         CCCCCAGGCCCTGCCAGCTTGGGCACCACGGCACTGTTCCCCCGAAAGGCCCAGCCACCC
                    ************************************************************

HsCD00329635        CAGGCCTTCCGCGGCGATGGCGTTCCCAGGGTGCTGGGGGGCCAGGAGCGGCCGGGGCCT
XM_005259512.1      CAGGCCTTCCGCGGCGATGGCGTTCCCAGGGTGCTGGGGGGCCAGGAGCGGCCGGGGCCT
NM_005224.2         CAGGCCTTCCGCGGCGATGGCGTTCCCAGGGTGCTGGGGGGCCAGGAGCGGCCGGGGCCT
                    ************************************************************

HsCD00329635        GGCCCTGCCCACCCCGGAGGGGCCGCCCACGTAGCCCCGCAGCTGCAGCCGCCTGACCAC
XM_005259512.1      GGCCCTGCCCACCCCGGAGGGGCCGCCCACGTAGCCCCGCAGCTGCAGCCGCCTGACCAC
NM_005224.2         GGCCCTGCCCACCCCGGAGGGGCCGCCCACGTAGCCCCGCAGCTGCAGCCGCCTGACCAC
                    ************************************************************

HsCD00329635        GGCGACTGGACTTACGAGGAGCAGTTTAAGCAGCTCTACGAACTCGACGGGGACCCCAAG
XM_005259512.1      GGCGACTGGACTTACGAGGAGCAGTTTAAGCAGCTCTACGAACTCGACGGGGACCCCAAG
NM_005224.2         GGCGACTGGACTTACGAGGAGCAGTTTAAGCAGCTCTACGAACTCGACGGGGACCCCAAG
                    ************************************************************

HsCD00329635        AGGAAGGAATTCCTGGATGACTTGTTCAGCTTCATGCAGAAGCGAGGGACACCTGTGAAC
XM_005259512.1      AGGAAGGAATTCCTGGATGACTTGTTCAGCTTCATGCAGAAGCGAGGGACACCTGTGAAC
NM_005224.2         AGGAAGGAATTCCTGGATGACTTGTTCAGCTTCATGCAGAAGCGAGGGACACCTGTGAAC
                    ************************************************************

HsCD00329635        CGCATCCCCATCATGGCCAAACAGGTCCTTGACCTGTTCATGCTGTACGTGCTGGTGACG
XM_005259512.1      CGCATCCCCATCATGGCCAAACAGGTCCTTGACCTGTTCATGCTGTACGTGCTGGTGACG
NM_005224.2         CGCATCCCCATCATGGCCAAACAGGTCCTTGACCTGTTCATGCTGTACGTGCTGGTGACG
                    ************************************************************

HsCD00329635        GAGAAGGGCGGCCTCGTGGAGGTCATCAACAAGAAGCTGTGGCGTGAGATCACCAAGGGC
XM_005259512.1      GAGAAGGGCGGCCTCGTGGAGGTCATCAACAAGAAGCTGTGGCGTGAGATCACCAAGGGC
NM_005224.2         GAGAAGGGCGGCCTCGTGGAGGTCATCAACAAGAAGCTGTGGCGTGAGATCACCAAGGGC
                    ************************************************************

HsCD00329635        CTCAACCTGCCCACGTCCATCACCAGTGCAGCCTTCACCCTGCGGACCCAATACATGGAG
XM_005259512.1      CTCAACCTGCCCACGTCCATCACCAGTGCAGCCTTCACCCTGCGGACCCAATACATGAAG
NM_005224.2         CTCAACCTGCCCACGTCCATCACCAGTGCAGCCTTCACCCTGCGGACCCAATACATGAAG
                    *********************************************************.**

HsCD00329635        TACCTGTACCCCTACGAGTGTGAGAAGCGGGGCCTCAGTAACCCCAATGAGCTCCAGGCA
XM_005259512.1      TACCTGTACCCCTACGAGTGTGAGAAGCGGGGCCTCAGTAACCCCAATGAGCTCCAGGCA
NM_005224.2         TACCTGTACCCCTACGAGTGTGAGAAGCGGGGCCTCAGTAACCCCAATGAGCTCCAGGCA
                    ************************************************************

HsCD00329635        GCCATAGACAGCAACCGACGGGAGGGCCGGCGCCAGAGCTTTGGTGGCTCCCTCTTTGCC
XM_005259512.1      GCCATAGACAGCAACCGACGGGAGGGCCGGCGCCAGAGCTTTGGTGGCTCCCTCTTTGCC
NM_005224.2         GCCATAGACAGCAACCGACGGGAGGGCCGGCGCCAGAGCTTTGGTGGCTCCCTCTTTGCC
                    ************************************************************

HsCD00329635        TACTCGCCAGGCGGGGCACACGGCATGCTCTCCTCACCCAAGCTACCCGTGTCCTCCCTG
XM_005259512.1      TACTCGCCAGGCGGGGCACACGGCATGCTCTCCTCACCCAAGCTACCCGTGTCCTCCCTG
NM_005224.2         TACTCGCCAGGCGGGGCACACGGCATGCTCTCCTCACCCAAGCTACCCGTGTCCTCCCTG
                    ************************************************************

HsCD00329635        GGCCTGGCCGCAAGCACCAACGGCAGCTCCATCACCCCCGCCCCTAAGATCAAGAAAGAG
XM_005259512.1      GGCCTGGCCGCAAGCACCAATGGCAGCTCCATCACCCCCGCCCCTAAGATCAAGAAAGAG
NM_005224.2         GGCCTGGCCGCAAGCACCAATGGCAGCTCCATCACCCCCGCCCCTAAGATCAAGAAAGAG
                    ******************** ***************************************

HsCD00329635        GAGGACTCAGCCATCCCCATCACAGTCCCTGGCCGCCTGCCTGTGTCCCTGGCGGGCCAC
XM_005259512.1      GAGGACTCAGCCATCCCCATCACAGTCCCTGGCCGCCTGCCTGTGTCCCTGGCGGGCCAC
NM_005224.2         GAGGACTCAGCCATCCCCATCACAGTCCCTGGCCGCCTGCCTGTGTCCCTGGCGGGCCAC
                    ************************************************************

HsCD00329635        CCTGTGGTGGCAGCCCAGGCAGCAGCTGTGCAAGCAGCAGCCGCCCAAGCAGCTGTGGCT
XM_005259512.1      CCTGTGGTGGCAGCCCAGGCAGCAGCTGTGCAAGCAGCAGCCGCCCAAGCAGCTGTGGCC
NM_005224.2         CCTGTGGTGGCAGCCCAGGCAGCAGCTGTGCAAGCAGCAGCCGCCCAAGCAGCTGTGGCC
                    *********************************************************** 

HsCD00329635        GCACAGGCAGCTGCCCTGGAACAGCTGCGGGAGAAGCTGGAGTCTGCGGAGCCTCCGGAG
XM_005259512.1      GCACAGGCAGCTGCCCTGGAACAGCTGCGGGAGAAGCTGGAGTCTGCAGAGCCTCCGGAG
NM_005224.2         GCACAGGCAGCTGCCCTGGAACAGCTGCGGGAGAAGCTGGAGTCTGCAGAGCCTCCGGAG
                    ***********************************************.************

HsCD00329635        AAGAAGATGGCCCTGGTGGCCGATGAGCAGCAACGGCTGATGCAACGTGCACTCCAGCAG
XM_005259512.1      AAGAAGATGGCCCTGGTGGCCGATGAGCAGCAACGGCTGATGCAACGTGCACTCCAGCAG
NM_005224.2         AAGAAGATGGCCCTGGTGGCCGATGAGCAGCAACGGCTGATGCAACGTGCACTCCAGCAG
                    ************************************************************

HsCD00329635        AACTTCCTGGCCATGGCGGCCCAGCTGCCCATGAGCATTCGGATCAACAGCCAAGCCTCC
XM_005259512.1      AACTTCCTGGCCATGGCGGCCCAGCTGCCCATGAGCATTCGGATCAACAGCCAAGCCTCC
NM_005224.2         AACTTCCTGGCCATGGCGGCCCAGCTGCCCATGAGCATTCGGATCAACAGCCAAGCCTCC
                    ************************************************************

HsCD00329635        GAAAGCCGCCAGGACTCTGCTGTGAACCTGACGGGCACCAACGGCAGCAACAGCATCAGC
XM_005259512.1      GAAAGCCGCCAGGACTCTGCTGTGAACCTGACGGGCACCAACGGCAGCAACAGCATCAGC
NM_005224.2         GAAAGCCGCCAGGACTCTGCTGTGAACCTGACGGGCACCAACGGCAGCAACAGCATCAGC
                    ************************************************************

HsCD00329635        ATGTCGGTGGAGATCAACGGCATCATGTACACAGGAGTTCTGTTTGCTCAGCCGCCGGCC
XM_005259512.1      ATGTCGGTGGAGATCAACGGCATCATGTACACAGGAGTTCTGTTTGCTCAGCCGCCGGCC
NM_005224.2         ATGTCGGTGGAGATCAACGGCATCATGTACACAGGAGTTCTGTTTGCTCAGCCGCCGGCC
                    ************************************************************

HsCD00329635        CCCACGCCAACCTCTGCTCCCAACAAAGGAGGCGGCGGCGGCGGCAGCAGCAGCAGCAAC
XM_005259512.1      CCCACGCCAACCTCTGCTCCCAACAAAGGAGGCGGCGGCGGCGGCGGCAGCAGCAGCAAC
NM_005224.2         CCCACGCCAACCTCTGCTCCCAACAAAGGAGGCGGCGGCGGCGGCGGCAGCAGCAGCAAC
                    *********************************************.**************

HsCD00329635        GCAGGCGGCCGGGGAGGAAACACCGGAACCAGCGGCGGCCAGGCTGGGCCAGCGGGGCTG
XM_005259512.1      GCAGGCGGCCGGGGAGGAAACACCGGAACCAGCGGCGGCCAGGCTGGGCCAGCGGGGCTG
NM_005224.2         GCAGGCGGCCGGGGAGGAAACACCGGAACCAGCGGCGGCCAGGCTGGGCCAGCGGGGCTG
                    ************************************************************

HsCD00329635        TCCACACCCTCCACATCTACCTCAAATAACTCGTTGCCTTTG
XM_005259512.1      TCCACACCCTCCACATCTACCTCAAATAACTCGTTGCCTTAA
NM_005224.2         TCCACACCCTCCACATCTACCTCAAATAACTCGTTGCCTTAA
                    ****************************************:.

Pathways

NCI : Direct p53 effectors

Clone

Cloning Information : 268084
HIP Master Clone ID : 193117
Original Clone ID : FLH268084.01L

Organism


TAX_ID : 9606
Species Specific ID: 1820

Genome

Chromosome : 19
Map Location : 19p13.3

Reagents

Labome : ARID3A-antibody