DNASU Plasmid Repository • 480.965.5697 | Email

ISL2 (Homo sapiens) in pLenti6.2/V5-DEST (V5-tagged lentiviral mammalian expression vector)

TOP

Explanation of Terms


Gene: Gene Symbol:  ISL2
Gene Name:  ISL LIM homeobox 2
Sequence             
Original Clone ID: FLH268090.01L
Keyword: None
Species: Homo sapiens
Type: cDNA
Vector Name: pLenti6.2/V5-DEST               Format:  FUSION
Source: HIP
Description: None
Comments: None
Mutation/
Discrepancy :
No / No
Publications: None
Authors:
HsCD00329641

Price:  Login for Pricing
AVAILABLE
No restriction
Special MTA: None

 

Insert sequence: 1080nts         Open reading frame : 1 to 1080
Reference Sequence Alignment

ATGGTGGATATTATTTTTCATTATCCTTTTCTGGGTGCTATGGGTGATCATTCCAAGAAG
AAGCCCGGGACGGCCATGTGCGTGGGCTGCGGGAGTCAGATCCACGACCAGTTTATCCTG
CGGGTGTCGCCCGACCTCGAGTGGCACGCGGCCTGCCTCAAGTGTGCCGAGTGCAGCCAG
TACCTGGACGAGACGTGCACGTGCTTCGTGAGAGACGGGAAGACCTACTGCAAGCGGGAC
TACGTCAGGCTGTTCGGCATCAAGTGCGCCAAGTGCCAGGTGGGCTTCAGCAGCAGCGAC
CTGGTGATGAGGGCGCGGGACAGCGTGTACCACATCGAGTGCTTCCGCTGCTCCGTGTGC
AGCCGCCAGCTGCTGCCTGGGGACGAGTTCTCGCTGCGGGAGCACGAGCTGCTCTGCCGC
GCCGACCACGGCCTCCTGCTCGAGCGCGCCGCGGCCGGCAGCCCGCGCAGCCCCGGCCCG
CTTCCCGGCGCCCGCGGCCTGCATCTGCCCGACGCTGGGTCGGGCCGGCAGCCCGCGTTG
CGCCCGCACGTGCACAAGCAGACGGAGAAGACGACCCGCGTGCGGACTGTGCTGAACGAG
AAGCAGCTGCACACTCTGCGGACCTGCTACGCCGCCAACCCGCGGCCCGACGCTCTCATG
AAGGAGCAGCTGGTGGAGATGACCGGCCTGAGCCCGCGGGTCATCCGCGTCTGGTTCCAG
AACAAGCGCTGCAAGGACAAGAAGAAATCCATTCTCATGAAGCAGCTGCAGCAGCAGCAG
CACAGCGACAAGACGAGCCTTCAGGGACTGACTGGGACGCCCCTGGTGGCGGGCAGTCCC
ATCCGCCATGAGAACGCCGTGCAGGGCAGCGCAGTGGAGGTGCAGACGTACCAGCCGCCG
TGGAAGGCGCTCAGCGAGTTTGCCCTCCAGAGCGACCTGGACCAACCCGCCTTCCAACAG
CTGGTCTCCTTCTCCGAGTCCGGCTCCCTAGGCAACTCCTCCGGCAGCGACGTGACCTCC
CTGTCCTCGCAGCTCCCGGACACCCCCAACAGTATGGTGCCGAGTCCCGTGGAGACGTTG


Coding Sequence Details
Insert Sequence Verified?: N Verification Method: Insert was fully sequenced in parent vector
Reference Sequence Annotations:
End on reference sequence 1158
Start on reference sequence 79
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
5p GTACAAAAAAGCAGGCTCCACC(atg-goi)

3' Linker Sequence:
5p (goi-stop)GACCCAGCTTTCTTGTAC

 

 Recommended Growth Condition:

Distributed in bacterial strain : DH5-alpha T1 phage resistant
Antibiotic Selection: Host Type:  bacterial    Marker:  ampicillin
Host Type:  mammalian    Marker:  blasticidin
Bacterial Selection Condition:  100 ug/mL ampicillin
Growth Condition:  Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:  Commonly used conditions for ampicillin resistant plasmid clones.
Protein Expression Results: None
Recommended expression in: Not Applicable
       DyNA Vector Map

         Powered by LabGenius

Vector Name: pLenti6.2/V5-DEST

pLenti6.2V5-DEST in advanced viewed

Synonyms: pLenti6.2/V5-DEST
Sequencing Primer: Forward:  CMV forward
Reverse:  V5 reverse
Description: Lentiviral mammalian expression vector;CMV promoter and C-terminal V5 tag; ampicillin resistance in bacteria; blasticidin resistance in mammalian cells; Gateway recombinational cloning.
Comments: None
Size (bp): 8813
Parent Vector: None
Empty Vector: None
Properties: Gateway, acceptor (destination), lentiviral infection, mammalian expression, recombinational cloning, with tag/fusion/marker
Author Name: Invitrogen
Publications: None
Vector Map:         Vector Sequence:
Sequence:

 



Vector Features:

Type Name Description Start Position End Position
bacterial origin pUC ori pUC origin of replication 7733 8406
death cassette ccdB ccdB death cassette (removed when insert is present) 3767 3976
packaging signal HIV-1 psi HIV-1 psi packaging signal 521 565
poly-A signal SV40 SV40 polyadenylation signal 5639 5770
promoter RSV RSV/5' LTR hybrid promoter 1 410
promoter CMV CMV promoter driving gene of interest 1809 2392
promoter PGK PGK promoter to drive selection marker in mammalian cells 4266 4771
recombination site attR2 attR2 recombinational cloning site 4127 4142
recombination site attR1 attR1 recombinational cloning site 2440 2456
response element HIV-1 RRE HIV-1 Rev response element (RRE) 1075 1308
selectable marker AmpR Ampicillin resistance gene for selection in bacteria 6728 7588
selectable marker BlastR Blasticidin resistance gene in mammalian cells 4849 5249
selectable marker CmR chloramphenicol resistance marker (removed in plasmids with insert) 2673 3329
splice acceptor site 3' splice acceptor two 3' splice acceptors at 1656 and 1684 1656 1656
splice donor site 5' splice donor 5' splice donor 520 520
tag V5 C-terminal V5 epitope tag 4197 4238

 

  • Gene
  • Protein
  • Clone
  • Organism
  • Genome
  • Reagents

Gene

 

Gene Symbol : ISL2
Symbol Nomenclature : ISL2
Designation : ISL2 transcription factor, LIM/homeodomain, (islet-2)|insulin gene enhancer protein ISL-2|islet-2
Full Nomenclature : ISL LIM homeobox 2
GENEID : 64843
HGNC : 18524
MIM : 609481
Vega : OTTHUMG00000143723
Target GenBank: BC012136

 

Reference Sequence Alignment

  

HsCD00329641      ATGGTGGATATTATTTTTCATTATCCTTTTCTGGGTGCTATGGGTGATCATTCCAAGAAG
NM_145805.1       ATGGTGGATATTATTTTTCATTATCCTTTTCTGGGTGCTATGGGTGATCATTCCAAGAAG
                  ************************************************************

HsCD00329641      AAGCCCGGGACGGCCATGTGCGTGGGCTGCGGGAGTCAGATCCACGACCAGTTTATCCTG
NM_145805.1       AAGCCCGGGACGGCCATGTGCGTGGGCTGCGGGAGTCAGATCCACGACCAGTTTATCCTG
                  ************************************************************

HsCD00329641      CGGGTGTCGCCCGACCTCGAGTGGCACGCGGCCTGCCTCAAGTGTGCCGAGTGCAGCCAG
NM_145805.1       CGGGTGTCGCCCGACCTCGAGTGGCACGCGGCCTGCCTCAAGTGTGCCGAGTGCAGCCAG
                  ************************************************************

HsCD00329641      TACCTGGACGAGACGTGCACGTGCTTCGTGAGAGACGGGAAGACCTACTGCAAGCGGGAC
NM_145805.1       TACCTGGACGAGACGTGCACGTGCTTCGTGAGAGACGGGAAGACCTACTGCAAGCGGGAC
                  ************************************************************

HsCD00329641      TACGTCAGGCTGTTCGGCATCAAGTGCGCCAAGTGCCAGGTGGGCTTCAGCAGCAGCGAC
NM_145805.1       TACGTCAGGCTGTTCGGCATCAAGTGCGCCAAGTGCCAGGTGGGCTTCAGCAGCAGCGAC
                  ************************************************************

HsCD00329641      CTGGTGATGAGGGCGCGGGACAGCGTGTACCACATCGAGTGCTTCCGCTGCTCCGTGTGC
NM_145805.1       CTGGTGATGAGGGCGCGGGACAGCGTGTACCACATCGAGTGCTTCCGCTGCTCCGTGTGC
                  ************************************************************

HsCD00329641      AGCCGCCAGCTGCTGCCTGGGGACGAGTTCTCGCTGCGGGAGCACGAGCTGCTCTGCCGC
NM_145805.1       AGCCGCCAGCTGCTGCCTGGGGACGAGTTCTCGCTGCGGGAGCACGAGCTGCTCTGCCGC
                  ************************************************************

HsCD00329641      GCCGACCACGGCCTCCTGCTCGAGCGCGCCGCGGCCGGCAGCCCGCGCAGCCCCGGCCCG
NM_145805.1       GCCGACCACGGCCTCCTGCTCGAGCGCGCCGCGGCCGGCAGCCCGCGCAGCCCCGGCCCG
                  ************************************************************

HsCD00329641      CTTCCCGGCGCCCGCGGCCTGCATCTGCCCGACGCTGGGTCGGGCCGGCAGCCCGCGTTG
NM_145805.1       CTTCCCGGCGCCCGCGGCCTGCATCTGCCCGACGCTGGGTCGGGCCGGCAGCCCGCGTTG
                  ************************************************************

HsCD00329641      CGCCCGCACGTGCACAAGCAGACGGAGAAGACGACCCGCGTGCGGACTGTGCTGAACGAG
NM_145805.1       CGCCCGCACGTGCACAAGCAGACGGAGAAGACGACCCGCGTGCGGACTGTGCTGAACGAG
                  ************************************************************

HsCD00329641      AAGCAGCTGCACACTCTGCGGACCTGCTACGCCGCCAACCCGCGGCCCGACGCTCTCATG
NM_145805.1       AAGCAGCTGCACACTCTGCGGACCTGCTACGCCGCCAACCCGCGGCCCGACGCTCTCATG
                  ************************************************************

HsCD00329641      AAGGAGCAGCTGGTGGAGATGACCGGCCTGAGCCCGCGGGTCATCCGCGTCTGGTTCCAG
NM_145805.1       AAGGAGCAGCTGGTGGAGATGACCGGCCTGAGCCCGCGGGTCATCCGCGTCTGGTTCCAG
                  ************************************************************

HsCD00329641      AACAAGCGCTGCAAGGACAAGAAGAAATCCATTCTCATGAAGCAGCTGCAGCAGCAGCAG
NM_145805.1       AACAAGCGCTGCAAGGACAAGAAGAAATCCATTCTCATGAAGCAGCTGCAGCAGCAGCAG
                  ************************************************************

HsCD00329641      CACAGCGACAAGACGAGCCTTCAGGGACTGACTGGGACGCCCCTGGTGGCGGGCAGTCCC
NM_145805.1       CACAGCGACAAGACGAGCCTTCAGGGACTGACTGGGACGCCCCTGGTGGCGGGCAGTCCC
                  ************************************************************

HsCD00329641      ATCCGCCATGAGAACGCCGTGCAGGGCAGCGCAGTGGAGGTGCAGACGTACCAGCCGCCG
NM_145805.1       ATCCGCCATGAGAACGCCGTGCAGGGCAGCGCAGTGGAGGTGCAGACGTACCAGCCGCCG
                  ************************************************************

HsCD00329641      TGGAAGGCGCTCAGCGAGTTTGCCCTCCAGAGCGACCTGGACCAACCCGCCTTCCAACAG
NM_145805.1       TGGAAGGCGCTCAGCGAGTTTGCCCTCCAGAGCGACCTGGACCAACCCGCCTTCCAACAG
                  ************************************************************

HsCD00329641      CTGGTCTCCTTCTCCGAGTCCGGCTCCCTAGGCAACTCCTCCGGCAGCGACGTGACCTCC
NM_145805.1       CTGGTCTCCTTCTCCGAGTCCGGCTCCCTAGGCAACTCCTCCGGCAGCGACGTGACCTCC
                  ************************************************************

HsCD00329641      CTGTCCTCGCAGCTCCCGGACACCCCCAACAGTATGGTGCCGAGTCCCGTGGAGACGTTG
NM_145805.1       CTGTCCTCGCAGCTCCCGGACACCCCCAACAGTATGGTGCCGAGTCCCGTGGAGACGTGA
                  ********************************************************** .

Protein

SMART domain : HOX : Homeodomain
SMART domain : LIM : Zinc-binding domain present in Lin-11, Isl-1, Mec-3.
UniProt : Q96A47
HPRD : 11053

Clone

Cloning Information : 268090
HIP Master Clone ID : 193123
Original Clone ID : FLH268090.01L

Organism


TAX_ID : 9606
Species Specific ID: 64843

Genome

Chromosome : 15
Map Location : 15q23
Ensembl : ENSG00000159556

Reagents

Labome : ISL2-antibody