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RBM4 (Homo sapiens) in pLenti6.2/V5-DEST (V5-tagged lentiviral mammalian expression vector)

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Explanation of Terms


Gene: Gene Symbol:  RBM4
Gene Name:  RNA binding motif protein 4
Sequence             
Original Clone ID: FLH268092.01L
Keyword: None
Species: Homo sapiens
Type: cDNA
Vector Name: pLenti6.2/V5-DEST               Format:  FUSION
Source: HIP
Description: None
Comments: None
Mutation/
Discrepancy :
No / No
Publications: None
Authors:
HsCD00329643

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AVAILABLE
No restriction
Special MTA: None

 

Insert sequence: 1095nts         Open reading frame : 1 to 1095
Reference Sequence Alignment

ATGGTGAAGCTGTTCATCGGAAACCTGCCCCGGGAGGCTACAGAGCAGGAGATTCGCTCA
CTCTTCGAGCAGTATGGGAAGGTGCTGGAATGTGACATCATTAAGAATTACGGCTTTGTG
CACATAGAAGACAAGACGGCAGCTGAGGATGCCATACGCAACCTGCACCATTACAAGCTT
CATGGGGTGAACATCAACGTGGAAGCCAGCAAGAATAAGAGCAAAACCTCAACAAAGTTG
CATGTGGGCAACATCAGTCCCACCTGCACCAATAAGGAGCTTCGAGCCAAGTTTGAGGAG
TATGGTCCGGTCATCGAATGTGACATCGTGAAAGATTATGCCTTCGTACACATGGAGCGG
GCAGAGGATGCAGTGGAGGCCATCAGGGGCCTTGATAACACAGAGTTTCAAGGCAAACGA
ATGCACGTGCAGTTGTCCACCAGCCGGCTTAGGACTGCGCCCGGGATGGGAGACCAGAGC
GGCTGCTATCGGTGCGGGAAAGAGGGGCACTGGTCCAAAGAGTGTCCGATAGATCGTTCA
GGCCGCGTGGCAGACTTGACCGAGCAATATAATGAGCAATACGGAGCAGTGCGTACGCCT
TACACCATGAGCTATGGGGATTCATTGTATTACAACAACGCGTACGGAGCGCTCGATGCC
TACTACAAGCGCTGCCGTGCTGCCCGGTCCTATGAGGCAGTGGCAGCTGCAGCTGCCTCC
GTGTATAATTACGCAGAGCAGACCCTGTCCCAGCTGCCACAAGTCCAGAATACAGCCATG
GCCAGTCACCTCACCTCCACCTCTCTCGATCCCTACGATAGACACCTGTTGCCGACCTCA
GGAGCTGCTGCCACAGCTGCTGCTGCAGCAGCAGCCGCTGCTGCTGTTACTGCAGCTTCC
ACTTCATATTACGGGCGGGATCGGAGCCCCCTGCGTCGCGCTACAGCCCCAGTCCCCACT
GTTGGAGAGGGCTACGGTTACGGGCATGAGAGTGAGTTGTCCCAAGCTTCAGCAGCCGCG
CGGAATTCTCTGTACGACATGGCCCGGTATGAGCGGGAGCAGTATGCCGATCGGGCGCGG
TACTCAGCCTTTTTG


Coding Sequence Details
Insert Sequence Verified?: N Verification Method: Insert was fully sequenced in parent vector
Reference Sequence Annotations:
End on reference sequence 1164
Start on reference sequence 70
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
5p GTACAAAAAAGCAGGCTCCACC(atg-goi)

3' Linker Sequence:
5p (goi-stop)GACCCAGCTTTCTTGTAC

 

 Recommended Growth Condition:

Distributed in bacterial strain : DH5-alpha T1 phage resistant
Antibiotic Selection: Host Type:  bacterial    Marker:  ampicillin
Host Type:  mammalian    Marker:  blasticidin
Bacterial Selection Condition:  100 ug/mL ampicillin
Growth Condition:  Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:  Commonly used conditions for ampicillin resistant plasmid clones.
Protein Expression Results: None
Recommended expression in: Not Applicable
       DyNA Vector Map

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Vector Name: pLenti6.2/V5-DEST

pLenti6.2V5-DEST in advanced viewed

Synonyms: pLenti6.2/V5-DEST
Sequencing Primer: Forward:  CMV forward
Reverse:  V5 reverse
Description: Lentiviral mammalian expression vector;CMV promoter and C-terminal V5 tag; ampicillin resistance in bacteria; blasticidin resistance in mammalian cells; Gateway recombinational cloning.
Comments: None
Size (bp): 8813
Parent Vector: None
Empty Vector: None
Properties: Gateway, acceptor (destination), lentiviral infection, mammalian expression, recombinational cloning, with tag/fusion/marker
Author Name: Invitrogen
Publications: None
Vector Map:         Vector Sequence:
Sequence:

 



Vector Features:

Type Name Description Start Position End Position
bacterial origin pUC ori pUC origin of replication 7733 8406
death cassette ccdB ccdB death cassette (removed when insert is present) 3767 3976
packaging signal HIV-1 psi HIV-1 psi packaging signal 521 565
poly-A signal SV40 SV40 polyadenylation signal 5639 5770
promoter RSV RSV/5' LTR hybrid promoter 1 410
promoter CMV CMV promoter driving gene of interest 1809 2392
promoter PGK PGK promoter to drive selection marker in mammalian cells 4266 4771
recombination site attR2 attR2 recombinational cloning site 4127 4142
recombination site attR1 attR1 recombinational cloning site 2440 2456
response element HIV-1 RRE HIV-1 Rev response element (RRE) 1075 1308
selectable marker AmpR Ampicillin resistance gene for selection in bacteria 6728 7588
selectable marker BlastR Blasticidin resistance gene in mammalian cells 4849 5249
selectable marker CmR chloramphenicol resistance marker (removed in plasmids with insert) 2673 3329
splice acceptor site 3' splice acceptor two 3' splice acceptors at 1656 and 1684 1656 1656
splice donor site 5' splice donor 5' splice donor 520 520
tag V5 C-terminal V5 epitope tag 4197 4238

 

  • Gene
  • Protein
  • Clone
  • Organism
  • Genome
  • Reagents

Gene

 

Gene Symbol : RBM4
Symbol Nomenclature : RBM4
SYNONYM : LARK; RBM4A; RP11-658F2.8; ZCCHC21; ZCRB3A
Designation : RNA-binding motif protein 4a|RNA-binding protein 4|lark homolog|transcriptional coactivator CoAZ|zinc finger CCHC-type and RNA binding motif 3A
Full Nomenclature : RNA binding motif protein 4
GENEID : 5936
HGNC : 9901
MIM : 602571
Vega : OTTHUMG00000154171
Target GenBank: BC032735

 

Reference Sequence Alignment

  

HsCD00329643      ATGGTGAAGCTGTTCATCGGAAACCTGCCCCGGGAGGCTACAGAGCAGGAGATTCGCTCA
NM_002896.3       ATGGTGAAGCTGTTCATCGGAAACCTGCCCCGGGAGGCTACAGAGCAGGAGATTCGCTCA
                  ************************************************************

HsCD00329643      CTCTTCGAGCAGTATGGGAAGGTGCTGGAATGTGACATCATTAAGAATTACGGCTTTGTG
NM_002896.3       CTCTTCGAGCAGTATGGGAAGGTGCTGGAATGTGACATCATTAAGAATTACGGCTTTGTG
                  ************************************************************

HsCD00329643      CACATAGAAGACAAGACGGCAGCTGAGGATGCCATACGCAACCTGCACCATTACAAGCTT
NM_002896.3       CACATAGAAGACAAGACGGCAGCTGAGGATGCCATACGCAACCTGCACCATTACAAGCTT
                  ************************************************************

HsCD00329643      CATGGGGTGAACATCAACGTGGAAGCCAGCAAGAATAAGAGCAAAACCTCAACAAAGTTG
NM_002896.3       CATGGGGTGAACATCAACGTGGAAGCCAGCAAGAATAAGAGCAAAACCTCAACAAAGTTG
                  ************************************************************

HsCD00329643      CATGTGGGCAACATCAGTCCCACCTGCACCAATAAGGAGCTTCGAGCCAAGTTTGAGGAG
NM_002896.3       CATGTGGGCAACATCAGTCCCACCTGCACCAATAAGGAGCTTCGAGCCAAGTTTGAGGAG
                  ************************************************************

HsCD00329643      TATGGTCCGGTCATCGAATGTGACATCGTGAAAGATTATGCCTTCGTACACATGGAGCGG
NM_002896.3       TATGGTCCGGTCATCGAATGTGACATCGTGAAAGATTATGCCTTCGTACACATGGAGCGG
                  ************************************************************

HsCD00329643      GCAGAGGATGCAGTGGAGGCCATCAGGGGCCTTGATAACACAGAGTTTCAAGGCAAACGA
NM_002896.3       GCAGAGGATGCAGTGGAGGCCATCAGGGGCCTTGATAACACAGAGTTTCAAGGCAAACGA
                  ************************************************************

HsCD00329643      ATGCACGTGCAGTTGTCCACCAGCCGGCTTAGGACTGCGCCCGGGATGGGAGACCAGAGC
NM_002896.3       ATGCACGTGCAGTTGTCCACCAGCCGGCTTAGGACTGCGCCCGGGATGGGAGACCAGAGC
                  ************************************************************

HsCD00329643      GGCTGCTATCGGTGCGGGAAAGAGGGGCACTGGTCCAAAGAGTGTCCGATAGATCGTTCA
NM_002896.3       GGCTGCTATCGGTGCGGGAAAGAGGGGCACTGGTCCAAAGAGTGTCCGATAGATCGTTCA
                  ************************************************************

HsCD00329643      GGCCGCGTGGCAGACTTGACCGAGCAATATAATGAGCAATACGGAGCAGTGCGTACGCCT
NM_002896.3       GGCCGCGTGGCAGACTTGACCGAGCAATATAATGAGCAATACGGAGCAGTGCGTACGCCT
                  ************************************************************

HsCD00329643      TACACCATGAGCTATGGGGATTCATTGTATTACAACAACGCGTACGGAGCGCTCGATGCC
NM_002896.3       TACACCATGAGCTATGGGGATTCATTGTATTACAACAACGCGTACGGAGCGCTCGATGCC
                  ************************************************************

HsCD00329643      TACTACAAGCGCTGCCGTGCTGCCCGGTCCTATGAGGCAGTGGCAGCTGCAGCTGCCTCC
NM_002896.3       TACTACAAGCGCTGCCGTGCTGCCCGGTCCTATGAGGCAGTGGCAGCTGCAGCTGCCTCC
                  ************************************************************

HsCD00329643      GTGTATAATTACGCAGAGCAGACCCTGTCCCAGCTGCCACAAGTCCAGAATACAGCCATG
NM_002896.3       GTGTATAATTACGCAGAGCAGACCCTGTCCCAGCTGCCACAAGTCCAGAATACAGCCATG
                  ************************************************************

HsCD00329643      GCCAGTCACCTCACCTCCACCTCTCTCGATCCCTACGATAGACACCTGTTGCCGACCTCA
NM_002896.3       GCCAGTCACCTCACCTCCACCTCTCTCGATCCCTACGATAGACACCTGTTGCCGACCTCA
                  ************************************************************

HsCD00329643      GGAGCTGCTGCCACAGCTGCTGCTGCAGCAGCAGCCGCTGCTGCTGTTACTGCAGCTTCC
NM_002896.3       GGAGCTGCTGCCACAGCTGCTGCTGCAGCAGCAGCCGCTGCTGCTGTTACTGCAGCTTCC
                  ************************************************************

HsCD00329643      ACTTCATATTACGGGCGGGATCGGAGCCCCCTGCGTCGCGCTACAGCCCCAGTCCCCACT
NM_002896.3       ACTTCATATTACGGGCGGGATCGGAGCCCCCTGCGTCGCGCTACAGCCCCAGTCCCCACT
                  ************************************************************

HsCD00329643      GTTGGAGAGGGCTACGGTTACGGGCATGAGAGTGAGTTGTCCCAAGCTTCAGCAGCCGCG
NM_002896.3       GTTGGAGAGGGCTACGGTTACGGGCATGAGAGTGAGTTGTCCCAAGCTTCAGCAGCCGCG
                  ************************************************************

HsCD00329643      CGGAATTCTCTGTACGACATGGCCCGGTATGAGCGGGAGCAGTATGCCGATCGGGCGCGG
NM_002896.3       CGGAATTCTCTGTACGACATGGCCCGGTATGAGCGGGAGCAGTATGCCGATCGGGCGCGG
                  ************************************************************

HsCD00329643      TACTCAGCCTTTTTG
NM_002896.3       TACTCAGCCTTTTAA
                  *************:.

Protein

SMART domain : ZnF_C2HC : zinc finger
SMART domain : RRM : RNA recognition motif
UniProt : E7EQS3
UniProt : Q9BWF3
UniProt : B4E1U0
HPRD : 03982

Clone

Cloning Information : 268092
HIP Master Clone ID : 193125
Original Clone ID : FLH268092.01L

Organism


TAX_ID : 9606
Species Specific ID: 5936

Genome

Chromosome : 11
Map Location : 11q13
Ensembl : ENSG00000173933

Reagents

Labome : RBM4-antibody