ZNF528 (Homo sapiens) in pLenti6.2/V5-DEST

Explanation of Terms

Gene:
Gene Symbol:  ZNF528
Gene Name:  zinc finger protein 528
Original Clone ID:
FLH268096.01L
Keyword:
None
Species:
Homo sapiens
Type:
cDNA
Vector Name:
pLenti6.2/V5-DEST
Format: 
FUSION
Source:
HIP
Description:
None
Comments:
None
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
HsCD00329647
Price: 
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AVAILABLE
No restriction
Special MTA: None
Insert sequence: 1887nts
Open reading frame: 1 to 1887

ATGGCCCTTACTCAGGGACCCTTGAAATTCATGGATGTGGCCATAGAGTTCTCTCAGGAA
GAGTGGAAATGCCTGGACCCTGCGCAGAGGACTTTATACAGGGACGTGATGTTGGAGAAT
TATAGGAACCTGGTCTCCCTGGGAATCTGTCTTCCTGACCTGAGTGTTACCTCCATGTTA
GAGCAAAAGAGAGATCCCTGGACTCTGCAGAGTGAAGAGAAAATAGCAAACGATCCAGAC
GGCAGGGAGTGCATCAAAGGTGTGAACACAGAGAGGAGCTCTAAATTGGGAAGTAATGCA
GGAAACAAGCCTTGTAAAAATCAACTTGGATTCACTTTTCAGTTACATCTGAGTGATCTA
CAGCTATTTCAAGCTGAAAGGAAAATTTCTGGGTGTAAGCATTTTGAAAAACCCGTCAGT
GACAATTCCTCAGTTTCACCGCTTGAAAAAATTTCTTCCAGTGTCAAATCCCACCTTTTA
AATAAATATAGAAATAATTTTGATCATGCTCCATTACTTCCACAAGAACAGAAAGCACAC
ATTAGGGAAAAAGCTTATAAATGTAATGAGCACGGCCAAGTCTTTAGAGCATCTGCAAGC
CTTACTAACCAAGTAATCCATAACGCAGATAATCCTTACAAATGCAGTGAATGTGGCAAG
GTCTTTAGTTGCAGTTCAAAGCTTGTGATACATCGAAGAATGCATACTGGAGAGAAGCCT
TACAAATGTCATGAATGTGGCAAGCTCTTCAGTAGCAATTCAAACCTTTCACAACATCAA
AGAATTCATACTGGAGAGAAGCCTTACAAATGTCATGAATGTGACAAGGTCTTTCGAAGC
AGTTCAAAGCTTGCACAACATCAAAGAATTCATACTGGAGAGAAGCCTTACAAATGTCAT
GAATGTGACAAGGTCTTCAATCAAATTGCACACCTTGTACGACATCAAAAAATTCATACT
GGAGAGAAACCTTACAGTTGTAATAAATGTGGCAAGGTCTTTAGTCGCCATTCATATCTA
GCAGAACATCAAACGGTTCATACTGGTGAGAAACCTTACAAATGTGAAGAATGTGGCAAA
GCATTTTCAGTGCGTTCCAGCCTCATAACCCATCAGTTAATTCACACTGGAAGGAAACCT
TACAAATGTAAAGAATGTGACAAGGTCTTTGGGCGCAAGTGTTTCCTGACCTCTCATCAG
AGAATTCATACTAGAGAGAGACCTTATGGATGCAGTCAGTGTGGCAAGATCTTTAGTCAG
AAATCAGACCTTATACGACATCGAAAAACTCATACTGATGAGAAGCCTTACAAATGTAAT
AAATGTGGCACAGCGTTTAGAGAGTTTTCAGACCTTACTGCCCATTTTCTAATCCATAGT
GGAGAGAAACCTTATGAATGTAAAGAATGTGGCAAAGTCTTCAGGTACAAGTCTTCTCTA
ACCAGTCATCATAGAATTCATACTGGAGAGAAGCCTTACAAATGTAACAGATGTGGCAAG
GTCTTCAGTCGCAGTTCAAACCTGGTATGCCATCAGAAAATTCATACAGGAGAAAAGCCT
TACAAATGTAATCAATGTGGCAAGGTCTTTAATCAAGCATCATACCTTACAAGACATCAA
ATAATTCATACTGGAGAGAGGCCTTACAGATGTAGTAAATGTGGCAAAGCATTTCGAGGG
TGTTCAGGCCTTACTGCCCATCTTGCAATCCATACTGAAAAGAAATCTCATGAGTGTAAA
GAATGTGGCAAGATCTTCACTCAGAAGTCTTCCCTCACCAATCACCATAGAATTCACATT
GGAGAGAAACCTTACAAATGCACCCTGTGCAGTAAGGTCTTCAGTCACAATTCTGACCTT
GCACAGCATCAGAGAGTTCATTCATTG

Protein sequence predicted based on nucleotide sequence

MALTQGPLKFMDVAIEFSQEEWKCLDPAQRTLYRDVMLENYRNLVSLGICLPDLSVTSML
EQKRDPWTLQSEEKIANDPDGRECIKGVNTERSSKLGSNAGNKPCKNQLGFTFQLHLSDL
QLFQAERKISGCKHFEKPVSDNSSVSPLEKISSSVKSHLLNKYRNNFDHAPLLPQEQKAH
IREKAYKCNEHGQVFRASASLTNQVIHNADNPYKCSECGKVFSCSSKLVIHRRMHTGEKP
YKCHECGKLFSSNSNLSQHQRIHTGEKPYKCHECDKVFRSSSKLAQHQRIHTGEKPYKCH
ECDKVFNQIAHLVRHQKIHTGEKPYSCNKCGKVFSRHSYLAEHQTVHTGEKPYKCEECGK
AFSVRSSLITHQLIHTGRKPYKCKECDKVFGRKCFLTSHQRIHTRERPYGCSQCGKIFSQ
KSDLIRHRKTHTDEKPYKCNKCGTAFREFSDLTAHFLIHSGEKPYECKECGKVFRYKSSL
TSHHRIHTGEKPYKCNRCGKVFSRSSNLVCHQKIHTGEKPYKCNQCGKVFNQASYLTRHQ
IIHTGERPYRCSKCGKAFRGCSGLTAHLAIHTEKKSHECKECGKIFTQKSSLTNHHRIHI
GEKPYKCTLCSKVFSHNSDLAQHQRVHSL

Coding Sequence Details

Insert Sequence Verified?:
N
Verification Method:
Insert was fully sequenced in parent vector
Reference Sequence Annotations:
End on reference sequence 1941
Start on reference sequence 55
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
5p GTACAAAAAAGCAGGCTCCACC(atg-goi)
3' Linker Sequence:
5p (goi-stop)GACCCAGCTTTCTTGTAC

Recommended Growth Condition:

Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:  bacterial    Marker:  ampicillin
Host Type:  mammalian    Marker:  blasticidin
Bacterial Selection Condition: 100 ug/mL ampicillin
Growth Condition: Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments: Commonly used conditions for ampicillin resistant plasmid clones.
Protein Expression Results:
None
Recommended expression in:
Not Applicable