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ZNF528 (Homo sapiens) in pLenti6.2/V5-DEST (V5-tagged lentiviral mammalian expression vector)

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Explanation of Terms


Gene: Gene Symbol:  ZNF528
Gene Name:  zinc finger protein 528
Sequence             
Original Clone ID: FLH268096.01L
Keyword: None
Species: Homo sapiens
Type: cDNA
Vector Name: pLenti6.2/V5-DEST               Format:  FUSION
Source: HIP
Description: None
Comments: None
Mutation/
Discrepancy :
No / No
Publications: None
Authors:
HsCD00329647

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AVAILABLE
No restriction
Special MTA: None

 

Insert sequence: 1887nts         Open reading frame : 1 to 1887
Reference Sequence Alignment

ATGGCCCTTACTCAGGGACCCTTGAAATTCATGGATGTGGCCATAGAGTTCTCTCAGGAA
GAGTGGAAATGCCTGGACCCTGCGCAGAGGACTTTATACAGGGACGTGATGTTGGAGAAT
TATAGGAACCTGGTCTCCCTGGGAATCTGTCTTCCTGACCTGAGTGTTACCTCCATGTTA
GAGCAAAAGAGAGATCCCTGGACTCTGCAGAGTGAAGAGAAAATAGCAAACGATCCAGAC
GGCAGGGAGTGCATCAAAGGTGTGAACACAGAGAGGAGCTCTAAATTGGGAAGTAATGCA
GGAAACAAGCCTTGTAAAAATCAACTTGGATTCACTTTTCAGTTACATCTGAGTGATCTA
CAGCTATTTCAAGCTGAAAGGAAAATTTCTGGGTGTAAGCATTTTGAAAAACCCGTCAGT
GACAATTCCTCAGTTTCACCGCTTGAAAAAATTTCTTCCAGTGTCAAATCCCACCTTTTA
AATAAATATAGAAATAATTTTGATCATGCTCCATTACTTCCACAAGAACAGAAAGCACAC
ATTAGGGAAAAAGCTTATAAATGTAATGAGCACGGCCAAGTCTTTAGAGCATCTGCAAGC
CTTACTAACCAAGTAATCCATAACGCAGATAATCCTTACAAATGCAGTGAATGTGGCAAG
GTCTTTAGTTGCAGTTCAAAGCTTGTGATACATCGAAGAATGCATACTGGAGAGAAGCCT
TACAAATGTCATGAATGTGGCAAGCTCTTCAGTAGCAATTCAAACCTTTCACAACATCAA
AGAATTCATACTGGAGAGAAGCCTTACAAATGTCATGAATGTGACAAGGTCTTTCGAAGC
AGTTCAAAGCTTGCACAACATCAAAGAATTCATACTGGAGAGAAGCCTTACAAATGTCAT
GAATGTGACAAGGTCTTCAATCAAATTGCACACCTTGTACGACATCAAAAAATTCATACT
GGAGAGAAACCTTACAGTTGTAATAAATGTGGCAAGGTCTTTAGTCGCCATTCATATCTA
GCAGAACATCAAACGGTTCATACTGGTGAGAAACCTTACAAATGTGAAGAATGTGGCAAA
GCATTTTCAGTGCGTTCCAGCCTCATAACCCATCAGTTAATTCACACTGGAAGGAAACCT
TACAAATGTAAAGAATGTGACAAGGTCTTTGGGCGCAAGTGTTTCCTGACCTCTCATCAG
AGAATTCATACTAGAGAGAGACCTTATGGATGCAGTCAGTGTGGCAAGATCTTTAGTCAG
AAATCAGACCTTATACGACATCGAAAAACTCATACTGATGAGAAGCCTTACAAATGTAAT
AAATGTGGCACAGCGTTTAGAGAGTTTTCAGACCTTACTGCCCATTTTCTAATCCATAGT
GGAGAGAAACCTTATGAATGTAAAGAATGTGGCAAAGTCTTCAGGTACAAGTCTTCTCTA
ACCAGTCATCATAGAATTCATACTGGAGAGAAGCCTTACAAATGTAACAGATGTGGCAAG
GTCTTCAGTCGCAGTTCAAACCTGGTATGCCATCAGAAAATTCATACAGGAGAAAAGCCT
TACAAATGTAATCAATGTGGCAAGGTCTTTAATCAAGCATCATACCTTACAAGACATCAA
ATAATTCATACTGGAGAGAGGCCTTACAGATGTAGTAAATGTGGCAAAGCATTTCGAGGG
TGTTCAGGCCTTACTGCCCATCTTGCAATCCATACTGAAAAGAAATCTCATGAGTGTAAA
GAATGTGGCAAGATCTTCACTCAGAAGTCTTCCCTCACCAATCACCATAGAATTCACATT
GGAGAGAAACCTTACAAATGCACCCTGTGCAGTAAGGTCTTCAGTCACAATTCTGACCTT
GCACAGCATCAGAGAGTTCATTCATTG


Coding Sequence Details
Insert Sequence Verified?: N Verification Method: Insert was fully sequenced in parent vector
Reference Sequence Annotations:
End on reference sequence 1941
Start on reference sequence 55
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
5p GTACAAAAAAGCAGGCTCCACC(atg-goi)

3' Linker Sequence:
5p (goi-stop)GACCCAGCTTTCTTGTAC

 

 Recommended Growth Condition:

Distributed in bacterial strain : DH5-alpha T1 phage resistant
Antibiotic Selection: Host Type:  bacterial    Marker:  ampicillin
Host Type:  mammalian    Marker:  blasticidin
Bacterial Selection Condition:  100 ug/mL ampicillin
Growth Condition:  Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:  Commonly used conditions for ampicillin resistant plasmid clones.
Protein Expression Results: None
Recommended expression in: Not Applicable
       DyNA Vector Map

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Vector Name: pLenti6.2/V5-DEST

pLenti6.2V5-DEST in advanced viewed

Synonyms: pLenti6.2/V5-DEST
Sequencing Primer: Forward:  CMV forward
Reverse:  V5 reverse
Description: Lentiviral mammalian expression vector;CMV promoter and C-terminal V5 tag; ampicillin resistance in bacteria; blasticidin resistance in mammalian cells; Gateway recombinational cloning.
Comments: None
Size (bp): 8813
Parent Vector: None
Empty Vector: None
Properties: Gateway, acceptor (destination), lentiviral infection, mammalian expression, recombinational cloning, with tag/fusion/marker
Author Name: Invitrogen
Publications: None
Vector Map:         Vector Sequence:
Sequence:

 



Vector Features:

Type Name Description Start Position End Position
bacterial origin pUC ori pUC origin of replication 7733 8406
death cassette ccdB ccdB death cassette (removed when insert is present) 3767 3976
packaging signal HIV-1 psi HIV-1 psi packaging signal 521 565
poly-A signal SV40 SV40 polyadenylation signal 5639 5770
promoter RSV RSV/5' LTR hybrid promoter 1 410
promoter CMV CMV promoter driving gene of interest 1809 2392
promoter PGK PGK promoter to drive selection marker in mammalian cells 4266 4771
recombination site attR2 attR2 recombinational cloning site 4127 4142
recombination site attR1 attR1 recombinational cloning site 2440 2456
response element HIV-1 RRE HIV-1 Rev response element (RRE) 1075 1308
selectable marker AmpR Ampicillin resistance gene for selection in bacteria 6728 7588
selectable marker BlastR Blasticidin resistance gene in mammalian cells 4849 5249
selectable marker CmR chloramphenicol resistance marker (removed in plasmids with insert) 2673 3329
splice acceptor site 3' splice acceptor two 3' splice acceptors at 1656 and 1684 1656 1656
splice donor site 5' splice donor 5' splice donor 520 520
tag V5 C-terminal V5 epitope tag 4197 4238

 

  • Gene
  • Pathways
  • Protein
  • Clone
  • Organism
  • Genome
  • Reagents

Gene

 

Gene Symbol : ZNF528
Symbol Nomenclature : ZNF528
Full Nomenclature : zinc finger protein 528
GENEID : 84436
HGNC : 29384
MIM : 615580
Vega : OTTHUMG00000156494
Target GenBank: BC111950

 

Reference Sequence Alignment

  

HsCD00329647        ------------------------------------------------------------
XM_005259316.1      ATGAAGAATGAGAGACCCATTAACAGAAGGCAAAGTAGAAGGATTCACTTCCAAAGAGAC
NM_032423.2         ------------------------------------------------------------
                                                                                

HsCD00329647        ------------------------------------------------ATGGCCCTTACT
XM_005259316.1      ATATTATGCAAGGAAGCAACTTGGAAGAGGAAAGAAAAGAAGTCAGGAATGGCCCTTACT
NM_032423.2         ------------------------------------------------ATGGCCCTTACT
                                                                    ************

HsCD00329647        CAGGGACCCTTGAAATTCATGGATGTGGCCATAGAGTTCTCTCAGGAAGAGTGGAAATGC
XM_005259316.1      CAGGGACCCTTGAAATTCATGGATGTGGCCATAGAGTTCTCTCAGGAAGAGTGGAAATGC
NM_032423.2         CAGGGACCCTTGAAATTCATGGATGTGGCCATAGAGTTCTCTCAGGAAGAGTGGAAATGC
                    ************************************************************

HsCD00329647        CTGGACCCTGCGCAGAGGACTTTATACAGGGACGTGATGTTGGAGAATTATAGGAACCTG
XM_005259316.1      CTGGACCCTGCGCAGAGGACTTTATACAGGGACGTGATGTTGGAGAATTATAGGAACCTG
NM_032423.2         CTGGACCCTGCGCAGAGGACTTTATACAGGGACGTGATGTTGGAGAATTATAGGAACCTG
                    ************************************************************

HsCD00329647        GTCTCCCTGGGAATCTGTCTTCCTGACCTGAGTGTTACCTCCATGTTAGAGCAAAAGAGA
XM_005259316.1      GTCTCCCTGGGAATCTGTCTTCCTGACCTGAGTGTTACCTCCATGTTAGAGCAAAAGAGA
NM_032423.2         GTCTCCCTGGGAATCTGTCTTCCTGACCTGAGTGTTACCTCCATGTTAGAGCAAAAGAGA
                    ************************************************************

HsCD00329647        GATCCCTGGACTCTGCAGAGTGAAGAGAAAATAGCAAACGATCCAGACGGCAGGGAGTGC
XM_005259316.1      GATCCCTGGACTCTGCAGAGTGAAGAGAAAATAGCAAACGATCCAGACGGCAGGGAGTGC
NM_032423.2         GATCCCTGGACTCTGCAGAGTGAAGAGAAAATAGCAAACGATCCAGACGGCAGGGAGTGC
                    ************************************************************

HsCD00329647        ATCAAAGGTGTGAACACAGAGAGGAGCTCTAAATTGGGAAGTAATGCAGGAAACAAGCCT
XM_005259316.1      ATCAAAGGTGTGAACACAGAGAGGAGCTCTAAATTGGGAAGTAATGCAGGAAACAAGCCT
NM_032423.2         ATCAAAGGTGTGAACACAGAGAGGAGCTCTAAATTGGGAAGTAATGCAGGAAACAAGCCT
                    ************************************************************

HsCD00329647        TGTAAAAATCAACTTGGATTCACTTTTCAGTTACATCTGAGTGATCTACAGCTATTTCAA
XM_005259316.1      TGTAAAAATCAACTTGGATTCACTTTTCAGTTACATCTGAGTGATCTACAGCTATTTCAA
NM_032423.2         TGTAAAAATCAACTTGGATTCACTTTTCAGTTACATCTGAGTGATCTACAGCTATTTCAA
                    ************************************************************

HsCD00329647        GCTGAAAGGAAAATTTCTGGGTGTAAGCATTTTGAAAAACCCGTCAGTGACAATTCCTCA
XM_005259316.1      GCTGAAAGGAAAATTTCTGGGTGTAAGCATTTTGAAAAACCCGTCAGTGACAATTCCTCA
NM_032423.2         GCTGAAAGGAAAATTTCTGGGTGTAAGCATTTTGAAAAACCCGTCAGTGACAATTCCTCA
                    ************************************************************

HsCD00329647        GTTTCACCGCTTGAAAAAATTTCTTCCAGTGTCAAATCCCACCTTTTAAATAAATATAGA
XM_005259316.1      GTTTCACCGCTTGAAAAAATTTCTTCCAGTGTCAAATCCCACCTTTTAAATAAATATAGA
NM_032423.2         GTTTCACCGCTTGAAAAAATTTCTTCCAGTGTCAAATCCCACCTTTTAAATAAATATAGA
                    ************************************************************

HsCD00329647        AATAATTTTGATCATGCTCCATTACTTCCACAAGAACAGAAAGCACACATTAGGGAAAAA
XM_005259316.1      AATAATTTTGATCATGCTCCATTACTTCCACAAGAACAGAAAGCACACATTAGGGAAAAA
NM_032423.2         AATAATTTTGATCATGCTCCATTACTTCCACAAGAACAGAAAGCACACATTAGGGAAAAA
                    ************************************************************

HsCD00329647        GCTTATAAATGTAATGAGCACGGCCAAGTCTTTAGAGCATCTGCAAGCCTTACTAACCAA
XM_005259316.1      GCTTATAAATGTAATGAGCACGGCCAAGTCTTTAGAGCATCTGCAAGCCTTACTAACCAA
NM_032423.2         GCTTATAAATGTAATGAGCACGGCCAAGTCTTTAGAGCATCTGCAAGCCTTACTAACCAA
                    ************************************************************

HsCD00329647        GTAATCCATAACGCAGATAATCCTTACAAATGCAGTGAATGTGGCAAGGTCTTTAGTTGC
XM_005259316.1      GTAATCCATAACGCAGATAATCCTTACAAATGCAGTGAATGTGGCAAGGTCTTTAGTTGC
NM_032423.2         GTAATCCATAACGCAGATAATCCTTACAAATGCAGTGAATGTGGCAAGGTCTTTAGTTGC
                    ************************************************************

HsCD00329647        AGTTCAAAGCTTGTGATACATCGAAGAATGCATACTGGAGAGAAGCCTTACAAATGTCAT
XM_005259316.1      AGTTCAAAGCTTGTGATACATCGAAGAATGCATACTGGAGAGAAGCCTTACAAATGTCAT
NM_032423.2         AGTTCAAAGCTTGTGATACATCGAAGAATGCATACTGGAGAGAAGCCTTACAAATGTCAT
                    ************************************************************

HsCD00329647        GAATGTGGCAAGCTCTTCAGTAGCAATTCAAACCTTTCACAACATCAAAGAATTCATACT
XM_005259316.1      GAATGTGGCAAGCTCTTCAGTAGCAATTCAAACCTTTCACAACATCAAAGAATTCATACT
NM_032423.2         GAATGTGGCAAGCTCTTCAGTAGCAATTCAAACCTTTCACAACATCAAAGAATTCATACT
                    ************************************************************

HsCD00329647        GGAGAGAAGCCTTACAAATGTCATGAATGTGACAAGGTCTTTCGAAGCAGTTCAAAGCTT
XM_005259316.1      GGAGAGAAGCCTTACAAATGTCATGAATGTGACAAGGTCTTTCGAAGCAGTTCAAAGCTT
NM_032423.2         GGAGAGAAGCCTTACAAATGTCATGAATGTGACAAGGTCTTTCGAAGCAGTTCAAAGCTT
                    ************************************************************

HsCD00329647        GCACAACATCAAAGAATTCATACTGGAGAGAAGCCTTACAAATGTCATGAATGTGACAAG
XM_005259316.1      GCACAACATCAAAGAATTCATACTGGAGAGAAGCCTTACAAATGTCATGAATGTGACAAG
NM_032423.2         GCACAACATCAAAGAATTCATACTGGAGAGAAGCCTTACAAATGTCATGAATGTGACAAG
                    ************************************************************

HsCD00329647        GTCTTCAATCAAATTGCACACCTTGTACGACATCAAAAAATTCATACTGGAGAGAAACCT
XM_005259316.1      GTCTTCAATCAAATTGCACACCTTGTACGACATCAAAAAATTCATACTGGAGAGAAACCT
NM_032423.2         GTCTTCAATCAAATTGCACACCTTGTACGACATCAAAAAATTCATACTGGAGAGAAACCT
                    ************************************************************

HsCD00329647        TACAGTTGTAATAAATGTGGCAAGGTCTTTAGTCGCCATTCATATCTAGCAGAACATCAA
XM_005259316.1      TACAGTTGTAATAAATGTGGCAAGGTCTTTAGTCGCCATTCATATCTAGCAGAACATCAA
NM_032423.2         TACAGTTGTAATAAATGTGGCAAGGTCTTTAGTCGCCATTCATATCTAGCAGAACATCAA
                    ************************************************************

HsCD00329647        ACGGTTCATACTGGTGAGAAACCTTACAAATGTGAAGAATGTGGCAAAGCATTTTCAGTG
XM_005259316.1      ACGGTTCATACTGGTGAGAAACCTTACAAATGTGAAGAATGTGGCAAAGCATTTTCAGTG
NM_032423.2         ACGGTTCATACTGGTGAGAAACCTTACAAATGTGAAGAATGTGGCAAAGCATTTTCAGTG
                    ************************************************************

HsCD00329647        CGTTCCAGCCTCATAACCCATCAGTTAATTCACACTGGAAGGAAACCTTACAAATGTAAA
XM_005259316.1      CGTTCCAGCCTCATAACCCATCAGTTAATTCACACTGGAAGGAAACCTTACAAATGTAAA
NM_032423.2         CGTTCCAGCCTCATAACCCATCAGTTAATTCACACTGGAAGGAAACCTTACAAATGTAAA
                    ************************************************************

HsCD00329647        GAATGTGACAAGGTCTTTGGGCGCAAGTGTTTCCTGACCTCTCATCAGAGAATTCATACT
XM_005259316.1      GAATGTGACAAGGTCTTTGGGCGCAAGTGTTTCCTGACCTCTCATCAGAGAATTCATACT
NM_032423.2         GAATGTGACAAGGTCTTTGGGCGCAAGTGTTTCCTGACCTCTCATCAGAGAATTCATACT
                    ************************************************************

HsCD00329647        AGAGAGAGACCTTATGGATGCAGTCAGTGTGGCAAGATCTTTAGTCAGAAATCAGACCTT
XM_005259316.1      AGAGAGAGACCTTATGGATGCAGTCAGTGTGGCAAGATCTTTAGTCAGAAATCAGACCTT
NM_032423.2         AGAGAGAGACCTTATGGATGCAGTCAGTGTGGCAAGATCTTTAGTCAGAAATCAGACCTT
                    ************************************************************

HsCD00329647        ATACGACATCGAAAAACTCATACTGATGAGAAGCCTTACAAATGTAATAAATGTGGCACA
XM_005259316.1      ATACGACATCGAAAAACTCATACTGATGAGAAGCCTTACAAATGTAATAAATGTGGCACA
NM_032423.2         ATACGACATCGAAAAACTCATACTGATGAGAAGCCTTACAAATGTAATAAATGTGGCACA
                    ************************************************************

HsCD00329647        GCGTTTAGAGAGTTTTCAGACCTTACTGCCCATTTTCTAATCCATAGTGGAGAGAAACCT
XM_005259316.1      GCGTTTAGAGAGTTTTCAGACCTTACTGCCCATTTTCTAATCCATAGTGGAGAGAAACCT
NM_032423.2         GCGTTTAGAGAGTTTTCAGACCTTACTGCCCATTTTCTAATCCATAGTGGAGAGAAACCT
                    ************************************************************

HsCD00329647        TATGAATGTAAAGAATGTGGCAAAGTCTTCAGGTACAAGTCTTCTCTAACCAGTCATCAT
XM_005259316.1      TATGAATGTAAAGAATGTGGCAAAGTCTTCAGGTACAAGTCTTCTCTAACCAGTCATCAT
NM_032423.2         TATGAATGTAAAGAATGTGGCAAAGTCTTCAGGTACAAGTCTTCTCTAACCAGTCATCAT
                    ************************************************************

HsCD00329647        AGAATTCATACTGGAGAGAAGCCTTACAAATGTAACAGATGTGGCAAGGTCTTCAGTCGC
XM_005259316.1      AGAATTCATACTGGAGAGAAGCCTTACAAATGTAACAGATGTGGCAAGGTCTTCAGTCGC
NM_032423.2         AGAATTCATACTGGAGAGAAGCCTTACAAATGTAACAGATGTGGCAAGGTCTTCAGTCGC
                    ************************************************************

HsCD00329647        AGTTCAAACCTGGTATGCCATCAGAAAATTCATACAGGAGAAAAGCCTTACAAATGTAAT
XM_005259316.1      AGTTCAAACCTGGTATGCCATCAGAAAATTCATACAGGAGAAAAGCCTTACAAATGTAAT
NM_032423.2         AGTTCAAACCTGGTATGCCATCAGAAAATTCATACAGGAGAAAAGCCTTACAAATGTAAT
                    ************************************************************

HsCD00329647        CAATGTGGCAAGGTCTTTAATCAAGCATCATACCTTACAAGACATCAAATAATTCATACT
XM_005259316.1      CAATGTGGCAAGGTCTTTAATCAAGCATCATACCTTACAAGACATCAAATAATTCATACT
NM_032423.2         CAATGTGGCAAGGTCTTTAATCAAGCATCATACCTTACAAGACATCAAATAATTCATACT
                    ************************************************************

HsCD00329647        GGAGAGAGGCCTTACAGATGTAGTAAATGTGGCAAAGCATTTCGAGGGTGTTCAGGCCTT
XM_005259316.1      GGAGAGAGGCCTTACAGATGTAGTAAATGTGGCAAAGCATTTCGAGGGTGTTCAGGCCTT
NM_032423.2         GGAGAGAGGCCTTACAGATGTAGTAAATGTGGCAAAGCATTTCGAGGGTGTTCAGGCCTT
                    ************************************************************

HsCD00329647        ACTGCCCATCTTGCAATCCATACTGAAAAGAAATCTCATGAGTGTAAAGAATGTGGCAAG
XM_005259316.1      ACTGCCCATCTTGCAATCCATACTGAAAAGAAATCTCATGAGTGTAAAGAATGTGGCAAG
NM_032423.2         ACTGCCCATCTTGCAATCCATACTGAAAAGAAATCTCATGAGTGTAAAGAATGTGGCAAG
                    ************************************************************

HsCD00329647        ATCTTCACTCAGAAGTCTTCCCTCACCAATCACCATAGAATTCACATTGGAGAGAAACCT
XM_005259316.1      ATCTTCACTCAGAAGTCTTCCCTCACCAATCACCATAGAATTCACATTGGAGAGAAACCT
NM_032423.2         ATCTTCACTCAGAAGTCTTCCCTCACCAATCACCATAGAATTCACATTGGAGAGAAACCT
                    ************************************************************

HsCD00329647        TACAAATGCACCCTGTGCAGTAAGGTCTTCAGTCACAATTCTGACCTTGCACAGCATCAG
XM_005259316.1      TACAAATGCACCCTGTGCAGTAAGGTCTTCAGTCACAATTCTGACCTTGCACAGCATCAG
NM_032423.2         TACAAATGCACCCTGTGCAGTAAGGTCTTCAGTCACAATTCTGACCTTGCACAGCATCAG
                    ************************************************************

HsCD00329647        AGAGTTCATTCATTG
XM_005259316.1      AGAGTTCATTCATGA
NM_032423.2         AGAGTTCATTCATGA
                    ************* .

Pathways

Reactome : Gene Expression
Reactome : Generic Transcription Pathway

Protein

SMART domain : KRAB : krueppel associated box
SMART domain : ZnF_C2H2 : zinc finger
UniProt : Q3MIS6
HPRD : 15820

Clone

Cloning Information : 268096
HIP Master Clone ID : 193130
Original Clone ID : FLH268096.01L

Organism


TAX_ID : 9606
Species Specific ID: 84436

Genome

Chromosome : 19
Map Location : 19q13
Ensembl : ENSG00000167555

Reagents

Labome : ZNF528-antibody