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ZNF549 (Homo sapiens) in pLenti6.2/V5-DEST (V5-tagged lentiviral mammalian expression vector)

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Explanation of Terms


Gene: Gene Symbol:  ZNF549
Gene Name:  zinc finger protein 549
Sequence             
Original Clone ID: FLH268099.01L
Keyword: None
Species: Homo sapiens
Type: cDNA
Vector Name: pLenti6.2/V5-DEST               Format:  FUSION
Source: HIP
Description: None
Comments: None
Mutation/
Discrepancy :
No / No
Publications: None
Authors:
HsCD00329650

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AVAILABLE
No restriction
Special MTA: None

 

Insert sequence: 1923nts         Open reading frame : 1 to 1923
Reference Sequence Alignment

ATGGCCGAGGCAGCGCTAGTGAATACGCCGCAGATTCCCATGGTAACAGAAGAGTTTGTG
AAACCATCACAGGGCCATGTGACCTTTGAGGATATTGCTGTGTACTTCTCCCAGGAGGAG
TGGGGCCTCCTTGATGAAGCTCAAAGGTGCCTGTATCATGATGTGATGCTGGAGAACTTT
TCGCTTATGGCCTCAGTAGGTTGTTTGCATGGAATAGAGGCTGAGGAGGCCCCTTCTGAG
CAGACTCTTTCTGCGCAAGGAGTGTCACAGGCCAGGACTCCAAAGCTAGGTCCTTCCATC
CCAAATGCTCATTCTTGTGAGATGTGTATCCTGGTCATGAAAGACATTTTGTACCTCAGT
GAGCATCAGGGGACACTTCCCTGGCAGAAACCTTATACGTCTGTGGCCAGTGGGAAATGG
TTTTCATTTGGTTCTAACCTGCAACAGCACCAGAACCAGGACAGTGGAGAGAAACACATC
AGAAAGGAGGAGAGCAGTGCCTTGCTTCTGAATAGCTGCAAAATTCCTCTGTCAGACAAT
CTTTTCCCATGCAAAGATGTTGAGAAGGATTTTCCAACCATCCTGGGCCTTCTCCAACAC
CAGACCACCCACAGCAGACAAGAGTATGCACATAGAAGCAGGGAGACCTTTCAACAAAGA
CGTTACAAATGTGAGCAAGTTTTCAATGAGAAAGTTCATGTTACTGAGCATCAGAGAGTC
CACACTGGAGAAAAAGCTTATAAGCGTAGGGAATATGGGAAATCCTTGAACTCTAAATAC
TTATTTGTTGAACACCAGAGAACCCATAATGCAGAAAAGCCTTATGTGTGCAATATATGT
GGGAAATCATTCCTCCATAAACAAACACTCGTTGGGCACCAGCAGAGAATTCACACTAGA
GAAAGGTCTTATGTGTGCATCGAATGTGGGAAATCCTTGAGCTCCAAATACTCACTTGTG
GAACACCAGAGAACCCATAATGGAGAAAAGCCTTATGTGTGCAATGTATGTGGGAAATCA
TTCCGCCACAAACAAACATTTGTTGGCCATCAGCAGAGAATCCACACTGGAGAGAGGCCT
TATGTGTGTATGGAATGTGGGAAATCTTTTATTCATTCCTATGACCGCATTCGACACCAG
AGAGTTCACACTGGAGAAGGGGCTTATCAGTGCAGTGAATGTGGGAAATCCTTCATATAC
AAACAGTCACTTCTTGATCACCATAGAATCCACACGGGAGAAAGGCCTTATGAGTGCAAA
GAATGTGGGAAGGCCTTCATTCACAAAAAAAGACTTCTTGAGCACCAGAGAATTCATACT
GGAGAAAAGCCTTATGTGTGCATCATATGTGGGAAATCATTTATCCGCTCGTCTGACTAC
ATGCGACACCAGAGAATTCACACTGGAGAAAGGGCTTATGAATGCAGTGACTGTGGGAAA
GCCTTCATCTCCAAACAAACACTTCTTAAGCATCACAAAATCCACACTAGAGAAAGGCCT
TATGAATGCAGTGAATGTGGAAAAGGCTTCTACCTTGAGGTTAAACTTCTTCAGCACCAA
AGAATCCATACTAGAGAACAACTTTGTGAGTGCAATGAATGTGGAAAAGTCTTCAGCCAC
CAAAAAAGACTTCTTGAGCACCAGAAAGTTCACACTGGCGAAAAGCCCTGTGAGTGCAGT
GAATGTGGGAAATGCTTTAGACACCGCACCAGCCTCATTCAACACCAGAAAGTTCACAGT
GGAGAGAGGCCTTATAACTGCACTGCATGTGAGAAGGCCTTTATCTATAAAAACAAACTT
GTTGAGCATCAGCGAATCCACACCGGAGAAAAGCCGTATGAATGTGGTAAATGTGGGAAA
GCCTTCAACAAAAGATATTCCCTTGTCAGGCACCAGAAGGTACATATAACAGAAGAGCCC
TTG


Coding Sequence Details
Insert Sequence Verified?: N Verification Method: Insert was fully sequenced in parent vector
Reference Sequence Annotations:
End on reference sequence 2076
Start on reference sequence 154
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
5p GTACAAAAAAGCAGGCTCCACC(atg-goi)

3' Linker Sequence:
5p (goi-stop)GACCCAGCTTTCTTGTAC

 

 Recommended Growth Condition:

Distributed in bacterial strain : DH5-alpha T1 phage resistant
Antibiotic Selection: Host Type:  bacterial    Marker:  ampicillin
Host Type:  mammalian    Marker:  blasticidin
Bacterial Selection Condition:  100 ug/mL ampicillin
Growth Condition:  Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:  Commonly used conditions for ampicillin resistant plasmid clones.
Protein Expression Results: None
Recommended expression in: Not Applicable
       DyNA Vector Map

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Vector Name: pLenti6.2/V5-DEST

pLenti6.2V5-DEST in advanced viewed

Synonyms: pLenti6.2/V5-DEST
Sequencing Primer: Forward:  CMV forward
Reverse:  V5 reverse
Description: Lentiviral mammalian expression vector;CMV promoter and C-terminal V5 tag; ampicillin resistance in bacteria; blasticidin resistance in mammalian cells; Gateway recombinational cloning.
Comments: None
Size (bp): 8813
Parent Vector: None
Empty Vector: None
Properties: Gateway, acceptor (destination), lentiviral infection, mammalian expression, recombinational cloning, with tag/fusion/marker
Author Name: Invitrogen
Publications: None
Vector Map:         Vector Sequence:
Sequence:

 



Vector Features:

Type Name Description Start Position End Position
bacterial origin pUC ori pUC origin of replication 7733 8406
death cassette ccdB ccdB death cassette (removed when insert is present) 3767 3976
packaging signal HIV-1 psi HIV-1 psi packaging signal 521 565
poly-A signal SV40 SV40 polyadenylation signal 5639 5770
promoter RSV RSV/5' LTR hybrid promoter 1 410
promoter CMV CMV promoter driving gene of interest 1809 2392
promoter PGK PGK promoter to drive selection marker in mammalian cells 4266 4771
recombination site attR2 attR2 recombinational cloning site 4127 4142
recombination site attR1 attR1 recombinational cloning site 2440 2456
response element HIV-1 RRE HIV-1 Rev response element (RRE) 1075 1308
selectable marker AmpR Ampicillin resistance gene for selection in bacteria 6728 7588
selectable marker BlastR Blasticidin resistance gene in mammalian cells 4849 5249
selectable marker CmR chloramphenicol resistance marker (removed in plasmids with insert) 2673 3329
splice acceptor site 3' splice acceptor two 3' splice acceptors at 1656 and 1684 1656 1656
splice donor site 5' splice donor 5' splice donor 520 520
tag V5 C-terminal V5 epitope tag 4197 4238

 

  • Gene
  • Pathways
  • Protein
  • Clone
  • Organism
  • Genome
  • Reagents

Gene

 

Gene Symbol : ZNF549
Symbol Nomenclature : ZNF549
Full Nomenclature : zinc finger protein 549
GENEID : 256051
HGNC : 26632
Vega : OTTHUMG00000183465
Target GenBank: BC060863

 

Reference Sequence Alignment

  

HsCD00329650        ATGGCCGAGGCAGCGCTAGTGAATACGCCGCAGATTCCCATGGTAACAGAAGAGTTTGTG
NM_001199295.1      ATGGCCGAGGCAGCGCTAGTGAATACGCCGCAGATTCCCATGGTAACAGAAGAGTTTGTG
NM_153263.2         ---------------------------------------ATGGCCGAGGCAGCGCTAGTG
                                                           **** ....*.**.* *:***

HsCD00329650        AAACCATCACAGGGCCATGTGACCTTTGAGGATATTGCTGTGTACTTCTCCCAGGAGGAG
NM_001199295.1      AAACCATCACAGGGCCATGTGACCTTTGAGGATATTGCTGTGTACTTCTCCCAGGAGGAG
NM_153263.2         AATACGCCGCAGGGCCATGTGACCTTTGAGGATATTGCTGTGTACTTCTCCCAGGAGGAG
                    **:.*. *.***************************************************

HsCD00329650        TGGGGCCTCCTTGATGAAGCTCAAAGGTGCCTGTATCATGATGTGATGCTGGAGAACTTT
NM_001199295.1      TGGGGCCTCCTTGATGAAGCTCAAAGGTGCCTGTATCATGATGTGATGCTGGAGAACTTT
NM_153263.2         TGGGGCCTCCTTGATGAAGCTCAAAGGTGCCTGTATCATGATGTGATGCTGGAGAACTTT
                    ************************************************************

HsCD00329650        TCGCTTATGGCCTCAGTAGGTTGTTTGCATGGAATAGAGGCTGAGGAGGCCCCTTCTGAG
NM_001199295.1      TCGCTTATGGCCTCAGTAGGTTGTTTGCATGGAATAGAGGCTGAGGAGGCCCCTTCTGAG
NM_153263.2         TCGCTTATGGCCTCAGTAGGTTGTTTGCATGGAATAGAGGCTGAGGAGGCCCCTTCTGAG
                    ************************************************************

HsCD00329650        CAGACTCTTTCTGCGCAAGGAGTGTCACAGGCCAGGACTCCAAAGCTAGGTCCTTCCATC
NM_001199295.1      CAGACTCTTTCTGCGCAAGGAGTGTCACAGGCCAGGACTCCAAAGCTAGGTCCTTCCATC
NM_153263.2         CAGACTCTTTCTGCGCAAGGAGTGTCACAGGCCAGGACTCCAAAGCTAGGTCCTTCCATC
                    ************************************************************

HsCD00329650        CCAAATGCTCATTCTTGTGAGATGTGTATCCTGGTCATGAAAGACATTTTGTACCTCAGT
NM_001199295.1      CCAAATGCTCATTCTTGTGAGATGTGTATCCTGGTCATGAAAGACATTTTGTACCTCAGT
NM_153263.2         CCAAATGCTCATTCTTGTGAGATGTGTATCCTGGTCATGAAAGACATTTTGTACCTCAGT
                    ************************************************************

HsCD00329650        GAGCATCAGGGGACACTTCCCTGGCAGAAACCTTATACGTCTGTGGCCAGTGGGAAATGG
NM_001199295.1      GAGCATCAGGGGACACTTCCCTGGCAGAAACCTTATACGTCTGTGGCCAGTGGGAAATGG
NM_153263.2         GAGCATCAGGGGACACTTCCCTGGCAGAAACCTTATACGTCTGTGGCCAGTGGGAAATGG
                    ************************************************************

HsCD00329650        TTTTCATTTGGTTCTAACCTGCAACAGCACCAGAACCAGGACAGTGGAGAGAAACACATC
NM_001199295.1      TTTTCATTTGGTTCTAACCTGCAACAGCACCAGAACCAGGACAGTGGAGAGAAACACATC
NM_153263.2         TTTTCATTTGGTTCTAACCTGCAACAGCACCAGAACCAGGACAGTGGAGAGAAACACATC
                    ************************************************************

HsCD00329650        AGAAAGGAGGAGAGCAGTGCCTTGCTTCTGAATAGCTGCAAAATTCCTCTGTCAGACAAT
NM_001199295.1      AGAAAGGAGGAGAGCAGTGCCTTGCTTCTGAATAGCTGCAAAATTCCTCTGTCAGACAAT
NM_153263.2         AGAAAGGAGGAGAGCAGTGCCTTGCTTCTGAATAGCTGCAAAATTCCTCTGTCAGACAAT
                    ************************************************************

HsCD00329650        CTTTTCCCATGCAAAGATGTTGAGAAGGATTTTCCAACCATCCTGGGCCTTCTCCAACAC
NM_001199295.1      CTTTTCCCATGCAAAGATGTTGAGAAGGATTTTCCAACCATCCTGGGCCTTCTCCAACAC
NM_153263.2         CTTTTCCCATGCAAAGATGTTGAGAAGGATTTTCCAACCATCCTGGGCCTTCTCCAACAC
                    ************************************************************

HsCD00329650        CAGACCACCCACAGCAGACAAGAGTATGCACATAGAAGCAGGGAGACCTTTCAACAAAGA
NM_001199295.1      CAGACCACCCACAGCAGACAAGAGTATGCACATAGAAGCAGGGAGACCTTTCAACAAAGA
NM_153263.2         CAGACCACCCACAGCAGACAAGAGTATGCACATAGAAGCAGGGAGACCTTTCAACAAAGA
                    ************************************************************

HsCD00329650        CGTTACAAATGTGAGCAAGTTTTCAATGAGAAAGTTCATGTTACTGAGCATCAGAGAGTC
NM_001199295.1      CGTTACAAATGTGAGCAAGTTTTCAATGAGAAAGTTCATGTTACTGAGCATCAGAGAGTC
NM_153263.2         CGTTACAAATGTGAGCAAGTTTTCAATGAGAAAGTTCATGTTACTGAGCATCAGAGAGTC
                    ************************************************************

HsCD00329650        CACACTGGAGAAAAAGCTTATAAGCGTAGGGAATATGGGAAATCCTTGAACTCTAAATAC
NM_001199295.1      CACACTGGAGAAAAAGCTTATAAGCGTAGGGAATATGGGAAATCCTTGAACTCTAAATAC
NM_153263.2         CACACTGGAGAAAAAGCTTATAAGCGTAGGGAATATGGGAAATCCTTGAACTCTAAATAC
                    ************************************************************

HsCD00329650        TTATTTGTTGAACACCAGAGAACCCATAATGCAGAAAAGCCTTATGTGTGCAATATATGT
NM_001199295.1      TTATTTGTTGAACACCAGAGAACCCATAATGCAGAAAAGCCTTATGTGTGCAATATATGT
NM_153263.2         TTATTTGTTGAACACCAGAGAACCCATAATGCAGAAAAGCCTTATGTGTGCAATATATGT
                    ************************************************************

HsCD00329650        GGGAAATCATTCCTCCATAAACAAACACTCGTTGGGCACCAGCAGAGAATTCACACTAGA
NM_001199295.1      GGGAAATCATTCCTCCATAAACAAACACTCGTTGGGCACCAGCAGAGAATTCACACTAGA
NM_153263.2         GGGAAATCATTCCTCCATAAACAAACACTCGTTGGGCACCAGCAGAGAATTCACACTAGA
                    ************************************************************

HsCD00329650        GAAAGGTCTTATGTGTGCATCGAATGTGGGAAATCCTTGAGCTCCAAATACTCACTTGTG
NM_001199295.1      GAAAGGTCTTATGTGTGCATCGAATGTGGGAAATCCTTGAGCTCCAAATACTCACTTGTG
NM_153263.2         GAAAGGTCTTATGTGTGCATCGAATGTGGGAAATCCTTGAGCTCCAAATACTCACTTGTG
                    ************************************************************

HsCD00329650        GAACACCAGAGAACCCATAATGGAGAAAAGCCTTATGTGTGCAATGTATGTGGGAAATCA
NM_001199295.1      GAACACCAGAGAACCCATAATGGAGAAAAGCCTTATGTGTGCAATGTATGTGGGAAATCA
NM_153263.2         GAACACCAGAGAACCCATAATGGAGAAAAGCCTTATGTGTGCAATGTATGTGGGAAATCA
                    ************************************************************

HsCD00329650        TTCCGCCACAAACAAACATTTGTTGGCCATCAGCAGAGAATCCACACTGGAGAGAGGCCT
NM_001199295.1      TTCCGCCACAAACAAACATTTGTTGGCCATCAGCAGAGAATCCACACTGGAGAGAGGCCT
NM_153263.2         TTCCGCCACAAACAAACATTTGTTGGCCATCAGCAGAGAATCCACACTGGAGAGAGGCCT
                    ************************************************************

HsCD00329650        TATGTGTGTATGGAATGTGGGAAATCTTTTATTCATTCCTATGACCGCATTCGACACCAG
NM_001199295.1      TATGTGTGTATGGAATGTGGGAAATCTTTTATTCATTCCTATGACCGCATTCGACACCAG
NM_153263.2         TATGTGTGTATGGAATGTGGGAAATCTTTTATTCATTCCTATGACCGCATTCGACACCAG
                    ************************************************************

HsCD00329650        AGAGTTCACACTGGAGAAGGGGCTTATCAGTGCAGTGAATGTGGGAAATCCTTCATATAC
NM_001199295.1      AGAGTTCACACTGGAGAAGGGGCTTATCAGTGCAGTGAATGTGGGAAATCCTTCATATAC
NM_153263.2         AGAGTTCACACTGGAGAAGGGGCTTATCAGTGCAGTGAATGTGGGAAATCCTTCATATAC
                    ************************************************************

HsCD00329650        AAACAGTCACTTCTTGATCACCATAGAATCCACACGGGAGAAAGGCCTTATGAGTGCAAA
NM_001199295.1      AAACAGTCACTTCTTGATCACCATAGAATCCACACGGGAGAAAGGCCTTATGAGTGCAAA
NM_153263.2         AAACAGTCACTTCTTGATCACCATAGAATCCACACGGGAGAAAGGCCTTATGAGTGCAAA
                    ************************************************************

HsCD00329650        GAATGTGGGAAGGCCTTCATTCACAAAAAAAGACTTCTTGAGCACCAGAGAATTCATACT
NM_001199295.1      GAATGTGGGAAGGCCTTCATTCACAAAAAAAGACTTCTTGAGCACCAGAGAATTCATACT
NM_153263.2         GAATGTGGGAAGGCCTTCATTCACAAAAAAAGACTTCTTGAGCACCAGAGAATTCATACT
                    ************************************************************

HsCD00329650        GGAGAAAAGCCTTATGTGTGCATCATATGTGGGAAATCATTTATCCGCTCGTCTGACTAC
NM_001199295.1      GGAGAAAAGCCTTATGTGTGCATCATATGTGGGAAATCATTTATCCGCTCGTCTGACTAC
NM_153263.2         GGAGAAAAGCCTTATGTGTGCATCATATGTGGGAAATCATTTATCCGCTCGTCTGACTAC
                    ************************************************************

HsCD00329650        ATGCGACACCAGAGAATTCACACTGGAGAAAGGGCTTATGAATGCAGTGACTGTGGGAAA
NM_001199295.1      ATGCGACACCAGAGAATTCACACTGGAGAAAGGGCTTATGAATGCAGTGACTGTGGGAAA
NM_153263.2         ATGCGACACCAGAGAATTCACACTGGAGAAAGGGCTTATGAATGCAGTGACTGTGGGAAA
                    ************************************************************

HsCD00329650        GCCTTCATCTCCAAACAAACACTTCTTAAGCATCACAAAATCCACACTAGAGAAAGGCCT
NM_001199295.1      GCCTTCATCTCCAAACAAACACTTCTTAAGCATCACAAAATCCACACTAGAGAAAGGCCT
NM_153263.2         GCCTTCATCTCCAAACAAACACTTCTTAAGCATCACAAAATCCACACTAGAGAAAGGCCT
                    ************************************************************

HsCD00329650        TATGAATGCAGTGAATGTGGAAAAGGCTTCTACCTTGAGGTTAAACTTCTTCAGCACCAA
NM_001199295.1      TATGAATGCAGTGAATGTGGAAAAGGCTTCTACCTTGAGGTTAAACTTCTTCAGCACCAA
NM_153263.2         TATGAATGCAGTGAATGTGGAAAAGGCTTCTACCTTGAGGTTAAACTTCTTCAGCACCAA
                    ************************************************************

HsCD00329650        AGAATCCATACTAGAGAACAACTTTGTGAGTGCAATGAATGTGGAAAAGTCTTCAGCCAC
NM_001199295.1      AGAATCCATACTAGAGAACAACTTTGTGAGTGCAATGAATGTGGAAAAGTCTTCAGCCAC
NM_153263.2         AGAATCCATACTAGAGAACAACTTTGTGAGTGCAATGAATGTGGAAAAGTCTTCAGCCAC
                    ************************************************************

HsCD00329650        CAAAAAAGACTTCTTGAGCACCAGAAAGTTCACACTGGCGAAAAGCCCTGTGAGTGCAGT
NM_001199295.1      CAAAAAAGACTTCTTGAGCACCAGAAAGTTCACACTGGCGAAAAGCCCTGTGAGTGCAGT
NM_153263.2         CAAAAAAGACTTCTTGAGCACCAGAAAGTTCACACTGGCGAAAAGCCCTGTGAGTGCAGT
                    ************************************************************

HsCD00329650        GAATGTGGGAAATGCTTTAGACACCGCACCAGCCTCATTCAACACCAGAAAGTTCACAGT
NM_001199295.1      GAATGTGGGAAATGCTTTAGACACCGCACCAGCCTCATTCAACACCAGAAAGTTCACAGT
NM_153263.2         GAATGTGGGAAATGCTTTAGACACCGCACCAGCCTCATTCAACACCAGAAAGTTCACAGT
                    ************************************************************

HsCD00329650        GGAGAGAGGCCTTATAACTGCACTGCATGTGAGAAGGCCTTTATCTATAAAAACAAACTT
NM_001199295.1      GGAGAGAGGCCTTATAACTGCACTGCATGTGAGAAGGCCTTTATCTATAAAAACAAACTT
NM_153263.2         GGAGAGAGGCCTTATAACTGCACTGCATGTGAGAAGGCCTTTATCTATAAAAACAAACTT
                    ************************************************************

HsCD00329650        GTTGAGCATCAGCGAATCCACACCGGAGAAAAGCCGTATGAATGTGGTAAATGTGGGAAA
NM_001199295.1      GTTGAGCATCAGCGAATCCACACCGGAGAAAAGCCGTATGAATGTGGTAAATGTGGGAAA
NM_153263.2         GTTGAGCATCAGCGAATCCACACCGGAGAAAAGCCGTATGAATGTGGTAAATGTGGGAAA
                    ************************************************************

HsCD00329650        GCCTTCAACAAAAGATATTCCCTTGTCAGGCACCAGAAGGTACATATAACAGAAGAGCCC
NM_001199295.1      GCCTTCAACAAAAGATATTCCCTTGTCAGGCACCAGAAGGTACATATAACAGAAGAGCCC
NM_153263.2         GCCTTCAACAAAAGATATTCCCTTGTCAGGCACCAGAAGGTACATATAACAGAAGAGCCC
                    ************************************************************

HsCD00329650        TTG
NM_001199295.1      TAG
NM_153263.2         TAG
                    *:*

Pathways

Reactome : Gene Expression
Reactome : Generic Transcription Pathway

Protein

SMART domain : KRAB : krueppel associated box
SMART domain : ZnF_C2H2 : zinc finger
UniProt : Q6P9A3
HPRD : 15833

Clone

Cloning Information : 268099
HIP Master Clone ID : 193133
Original Clone ID : FLH268099.01L

Organism


TAX_ID : 9606
Species Specific ID: 256051

Genome

Chromosome : 19
Map Location : 19q13.43
Ensembl : ENSG00000121406

Reagents

Labome : ZNF549-antibody