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LHX1 (Homo sapiens) in pLenti6.2/V5-DEST (V5-tagged lentiviral mammalian expression vector)

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Explanation of Terms


Gene: Gene Symbol:  LHX1
Gene Name:  LIM homeobox 1
Sequence             
Original Clone ID: FLH268100.01L
Keyword: None
Species: Homo sapiens
Type: cDNA
Vector Name: pLenti6.2/V5-DEST               Format:  FUSION
Source: HIP
Description: None
Comments: None
Mutation/
Discrepancy :
No / No
Publications: None
Authors:
HsCD00329651

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AVAILABLE
No restriction
Special MTA: None

 

Insert sequence: 1221nts         Open reading frame : 1 to 1221
Reference Sequence Alignment

ATGGTTCACTGTGCCGGCTGCAAAAGGCCCATCCTGGACCGCTTTCTCTTGAACGTGCTG
GACAGGGCCTGGCACGTCAAGTGCGTCCAGTGCTGTGAATGTAAATGCAACCTGACCGAG
AAGTGCTTCTCCAGGGAAGGCAAACTCTACTGCAAGAACGACTTCTTCCGGTGTTTCGGT
ACCAAATGCGCAGGCTGCGCTCAGGGCATCTCCCCTAGCGACCTGGTGCGGAGAGCGCGG
AGCAAAGTGTTTCACCTGAACTGCTTCACCTGCATGATGTGTAACAAGCAGCTCTCCACT
GGCGAGGAACTCTACATCATCGACGAGAATAAGTTCGTCTGCAAAGAGGATTACCTAAGT
AACAGCAGTGTTGCCAAAGAGAACAGCCTTCACTCGGCCACCACGGGCAGTGACCCCAGT
TTGTCTCCGGATTCCCAAGACCCGTCGCAGGACGACGCCAAGGACTCGGAGAGCGCCAAC
GTGTCGGACAAGGAAGCGGGTAGCAACGAGAATGACGACCAGAACCTGGGCGCCAAGCGG
CGGGGACCGCGCACCACCATCAAAGCCAAGCAGCTGGAGACGCTGAAGGCCGCCTTCGCT
GCTACACCCAAGCCCACCCGCCACATCCGCGAGCAGCTGGCGCAGGAGACCGGCCTCAAC
ATGCGCGTCATTCAGGTCTGGTTCCAGAACCGGCGCTCCAAGGAGCGGAGGATGAAGCAG
CTGAGCGCCCTGGGCGCCCGGCGCCACGCCTTCTTCCGCAGTCCGCGCCGGATGCGGCCG
CTGGTGGACCGCCTGGAGCCGGGCGAGCTCATCCCCAATGGTCCCTTCTCCTTCTACGGA
GATTACCAGAGCGAGTACTACGGGCCCGGGGGCAACTACGACTTCTTCCCGCAAGGCCCC
CCGTCCTCGCAGGCCCAGACACCAGTGGACCTACCCTTCGTGCCGTCATCTGGGCCGTCC
GGGACGCCCCTGGGTGGCCTGGAGCACCCGCTGCCGGGCCACCACCCGTCGAGCGAGGCG
CAGCGGTTTACCGACATCCTGGCGCACCCACCCGGGGACTCGCCCAGCCCCGAGCCCAGC
CTGCCCGGGCCTCTGCACTCCATGTCGGCCGAGGTCTTCGGACCCAGCCCGCCCTTCTCG
TCGCTGTCGGTCAACGGTGGGGCGAGCTACGGAAACCACCTGTCCCACCCCCCCGAAATG
AACGAGGCGGCCGTGTGGTTG


Coding Sequence Details
Insert Sequence Verified?: N Verification Method: Insert was fully sequenced in parent vector
Reference Sequence Annotations:
End on reference sequence 1313
Start on reference sequence 93
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
5p GTACAAAAAAGCAGGCTCCACC(atg-goi)

3' Linker Sequence:
5p (goi-stop)GACCCAGCTTTCTTGTAC

 

 Recommended Growth Condition:

Distributed in bacterial strain : DH5-alpha T1 phage resistant
Antibiotic Selection: Host Type:  bacterial    Marker:  ampicillin
Host Type:  mammalian    Marker:  blasticidin
Bacterial Selection Condition:  100 ug/mL ampicillin
Growth Condition:  Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:  Commonly used conditions for ampicillin resistant plasmid clones.
Protein Expression Results: None
Recommended expression in: Not Applicable
       DyNA Vector Map

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Vector Name: pLenti6.2/V5-DEST

pLenti6.2V5-DEST in advanced viewed

Synonyms: pLenti6.2/V5-DEST
Sequencing Primer: Forward:  CMV forward
Reverse:  V5 reverse
Description: Lentiviral mammalian expression vector;CMV promoter and C-terminal V5 tag; ampicillin resistance in bacteria; blasticidin resistance in mammalian cells; Gateway recombinational cloning.
Comments: None
Size (bp): 8813
Parent Vector: None
Empty Vector: None
Properties: Gateway, acceptor (destination), lentiviral infection, mammalian expression, recombinational cloning, with tag/fusion/marker
Author Name: Invitrogen
Publications: None
Vector Map:         Vector Sequence:
Sequence:

 



Vector Features:

Type Name Description Start Position End Position
bacterial origin pUC ori pUC origin of replication 7733 8406
death cassette ccdB ccdB death cassette (removed when insert is present) 3767 3976
packaging signal HIV-1 psi HIV-1 psi packaging signal 521 565
poly-A signal SV40 SV40 polyadenylation signal 5639 5770
promoter RSV RSV/5' LTR hybrid promoter 1 410
promoter CMV CMV promoter driving gene of interest 1809 2392
promoter PGK PGK promoter to drive selection marker in mammalian cells 4266 4771
recombination site attR2 attR2 recombinational cloning site 4127 4142
recombination site attR1 attR1 recombinational cloning site 2440 2456
response element HIV-1 RRE HIV-1 Rev response element (RRE) 1075 1308
selectable marker AmpR Ampicillin resistance gene for selection in bacteria 6728 7588
selectable marker BlastR Blasticidin resistance gene in mammalian cells 4849 5249
selectable marker CmR chloramphenicol resistance marker (removed in plasmids with insert) 2673 3329
splice acceptor site 3' splice acceptor two 3' splice acceptors at 1656 and 1684 1656 1656
splice donor site 5' splice donor 5' splice donor 520 520
tag V5 C-terminal V5 epitope tag 4197 4238

 

  • Gene
  • Pathways
  • Protein
  • Clone
  • Organism
  • Genome
  • Reagents

Gene

 

Gene Symbol : LHX1
Symbol Nomenclature : LHX1
SYNONYM : LIM-1; LIM1
Designation : LIM homeobox protein 1|LIM/homeobox protein Lhx1|homeobox protein Lim-1
Full Nomenclature : LIM homeobox 1
GENEID : 3975
HGNC : 6593
MIM : 601999
Vega : OTTHUMG00000133068
Target GenBank: BC111936

 

Reference Sequence Alignment

  

HsCD00329651      ATGGTTCACTGTGCCGGCTGCAAAAGGCCCATCCTGGACCGCTTTCTCTTGAACGTGCTG
NM_005568.3       ATGGTTCACTGTGCCGGCTGCAAAAGGCCCATCCTGGACCGCTTTCTCTTGAACGTGCTG
                  ************************************************************

HsCD00329651      GACAGGGCCTGGCACGTCAAGTGCGTCCAGTGCTGTGAATGTAAATGCAACCTGACCGAG
NM_005568.3       GACAGGGCCTGGCACGTCAAGTGCGTCCAGTGCTGTGAATGTAAATGCAACCTGACCGAG
                  ************************************************************

HsCD00329651      AAGTGCTTCTCCAGGGAAGGCAAACTCTACTGCAAGAACGACTTCTTCCGGTGTTTCGGT
NM_005568.3       AAGTGCTTCTCCAGGGAAGGCAAACTCTACTGCAAGAACGACTTCTTCCGGTGTTTCGGT
                  ************************************************************

HsCD00329651      ACCAAATGCGCAGGCTGCGCTCAGGGCATCTCCCCTAGCGACCTGGTGCGGAGAGCGCGG
NM_005568.3       ACCAAATGCGCAGGCTGCGCTCAGGGCATCTCCCCTAGCGACCTGGTGCGGAGAGCGCGG
                  ************************************************************

HsCD00329651      AGCAAAGTGTTTCACCTGAACTGCTTCACCTGCATGATGTGTAACAAGCAGCTCTCCACT
NM_005568.3       AGCAAAGTGTTTCACCTGAACTGCTTCACCTGCATGATGTGTAACAAGCAGCTCTCCACT
                  ************************************************************

HsCD00329651      GGCGAGGAACTCTACATCATCGACGAGAATAAGTTCGTCTGCAAAGAGGATTACCTAAGT
NM_005568.3       GGCGAGGAACTCTACATCATCGACGAGAATAAGTTCGTCTGCAAAGAGGATTACCTAAGT
                  ************************************************************

HsCD00329651      AACAGCAGTGTTGCCAAAGAGAACAGCCTTCACTCGGCCACCACGGGCAGTGACCCCAGT
NM_005568.3       AACAGCAGTGTTGCCAAAGAGAACAGCCTTCACTCGGCCACCACGGGCAGTGACCCCAGT
                  ************************************************************

HsCD00329651      TTGTCTCCGGATTCCCAAGACCCGTCGCAGGACGACGCCAAGGACTCGGAGAGCGCCAAC
NM_005568.3       TTGTCTCCGGATTCCCAAGACCCGTCGCAGGACGACGCCAAGGACTCGGAGAGCGCCAAC
                  ************************************************************

HsCD00329651      GTGTCGGACAAGGAAGCGGGTAGCAACGAGAATGACGACCAGAACCTGGGCGCCAAGCGG
NM_005568.3       GTGTCGGACAAGGAAGCGGGTAGCAACGAGAATGACGACCAGAACCTGGGCGCCAAGCGG
                  ************************************************************

HsCD00329651      CGGGGACCGCGCACCACCATCAAAGCCAAGCAGCTGGAGACGCTGAAGGCCGCCTTCGCT
NM_005568.3       CGGGGACCGCGCACCACCATCAAAGCCAAGCAGCTGGAGACGCTGAAGGCCGCCTTCGCT
                  ************************************************************

HsCD00329651      GCTACACCCAAGCCCACCCGCCACATCCGCGAGCAGCTGGCGCAGGAGACCGGCCTCAAC
NM_005568.3       GCTACACCCAAGCCCACCCGCCACATCCGCGAGCAGCTGGCGCAGGAGACCGGCCTCAAC
                  ************************************************************

HsCD00329651      ATGCGCGTCATTCAGGTCTGGTTCCAGAACCGGCGCTCCAAGGAGCGGAGGATGAAGCAG
NM_005568.3       ATGCGCGTCATTCAGGTCTGGTTCCAGAACCGGCGCTCCAAGGAGCGGAGGATGAAGCAG
                  ************************************************************

HsCD00329651      CTGAGCGCCCTGGGCGCCCGGCGCCACGCCTTCTTCCGCAGTCCGCGCCGGATGCGGCCG
NM_005568.3       CTGAGCGCCCTGGGCGCCCGGCGCCACGCCTTCTTCCGCAGTCCGCGCCGGATGCGGCCG
                  ************************************************************

HsCD00329651      CTGGTGGACCGCCTGGAGCCGGGCGAGCTCATCCCCAATGGTCCCTTCTCCTTCTACGGA
NM_005568.3       CTGGTGGACCGCCTGGAGCCGGGCGAGCTCATCCCCAATGGTCCCTTCTCCTTCTACGGA
                  ************************************************************

HsCD00329651      GATTACCAGAGCGAGTACTACGGGCCCGGGGGCAACTACGACTTCTTCCCGCAAGGCCCC
NM_005568.3       GATTACCAGAGCGAGTACTACGGGCCCGGGGGCAACTACGACTTCTTCCCGCAAGGCCCC
                  ************************************************************

HsCD00329651      CCGTCCTCGCAGGCCCAGACACCAGTGGACCTACCCTTCGTGCCGTCATCTGGGCCGTCC
NM_005568.3       CCGTCCTCGCAGGCCCAGACACCAGTGGACCTACCCTTCGTGCCGTCATCTGGGCCGTCC
                  ************************************************************

HsCD00329651      GGGACGCCCCTGGGTGGCCTGGAGCACCCGCTGCCGGGCCACCACCCGTCGAGCGAGGCG
NM_005568.3       GGGACGCCCCTGGGTGGCCTGGAGCACCCGCTGCCGGGCCACCACCCGTCGAGCGAGGCG
                  ************************************************************

HsCD00329651      CAGCGGTTTACCGACATCCTGGCGCACCCACCCGGGGACTCGCCCAGCCCCGAGCCCAGC
NM_005568.3       CAGCGGTTTACCGACATCCTGGCGCACCCACCCGGGGACTCGCCCAGCCCCGAGCCCAGC
                  ************************************************************

HsCD00329651      CTGCCCGGGCCTCTGCACTCCATGTCGGCCGAGGTCTTCGGACCCAGCCCGCCCTTCTCG
NM_005568.3       CTGCCCGGGCCTCTGCACTCCATGTCGGCCGAGGTCTTCGGACCCAGCCCGCCCTTCTCG
                  ************************************************************

HsCD00329651      TCGCTGTCGGTCAACGGTGGGGCGAGCTACGGAAACCACCTGTCCCACCCCCCCGAAATG
NM_005568.3       TCGCTGTCGGTCAACGGTGGGGCGAGCTACGGAAACCACCTGTCCCACCCCCCCGAAATG
                  ************************************************************

HsCD00329651      AACGAGGCGGCCGTGTGGTTG
NM_005568.3       AACGAGGCGGCCGTGTGGTAG
                  *******************:*

Pathways

WikiPathway : Neural Crest Differentiation

Protein

SMART domain : HOX : Homeodomain
SMART domain : LIM : Zinc-binding domain present in Lin-11, Isl-1, Mec-3.
UniProt : Q58F18
UniProt : P48742
HPRD : 09067

Clone

Cloning Information : 268100
HIP Master Clone ID : 193134
Original Clone ID : FLH268100.01L

Organism


TAX_ID : 9606
Species Specific ID: 3975

Genome

Chromosome : 17
Map Location : 17q12
Ensembl : ENSG00000132130

Reagents

Labome : LHX1-antibody