DNASU Plasmid Repository • 480.965.5697 | Email

ZNF561 (Homo sapiens) in pLenti6.2/V5-DEST (V5-tagged lentiviral mammalian expression vector)

TOP

Explanation of Terms


Gene: Gene Symbol:  ZNF561
Gene Name:  zinc finger protein 561
Sequence             
Original Clone ID: FLH268105.01L
Keyword: None
Species: Homo sapiens
Type: cDNA
Vector Name: pLenti6.2/V5-DEST               Format:  FUSION
Source: HIP
Description: None
Comments: None
Mutation/
Discrepancy :
No / No
Publications: None
Authors:
HsCD00329655

Price:  Login for Pricing
AVAILABLE
No restriction
Special MTA: None

 

Insert sequence: 1254nts         Open reading frame : 1 to 1254
Reference Sequence Alignment

ATGCTGGAGAACTACATGAACCTGGCCTCTGTGGAATGGGAAATACAACCTAGAACCAAA
CGGTCATCACTTCAGCAGGGTTTTTTGAAGAATCAAATATTCAGTGGGATACAAATGACA
AGAGGCTACAGTGGATGGAAACTCTGTGACTGTAAGAATTGTGGAGAGGTCTTCAGGGAA
CAGTTTTGCCTTAAGACACACATGAGAGTTCAGAATGGAGGGAATACTTCTGAGGGTAAT
TGTTATGGAAAAGACACCCTCAGTGTGCACAAGGAAGCCTCTACTGGACAGGAACTTTCC
AAATTTAATCCATGTGGAAAAGTCTTTACTCTAACTCCAGGTCTTGCTGTACATCTTGAA
GTTCTCAATGCAAGACAACCCTACAAATGTAAGGAATGTGGAAAAGGCTTTAAGTATTTT
GCAAGCCTTGATAATCATATGGGAATCCACACTGATGAGAAACTCTGTGAATTTCAGGAA
TATGGGAGAGCTGTCACAGCTTCTTCACACCTAAAGCAGTGTGTAGCAGTTCATACAGGA
AAGAAATCCAAAAAGACTAAGAAATGTGGGAAATCCTTCACTAATTTTTCTCAACTTTAT
GCACCTGTGAAAACTCATAAAGGAGAGAAGTCCTTTGAATGTAAAGAATGTGGAAGATCC
TTTAGAAATTCCTCATGCCTTAATGATCACATTCAAATTCACACTGGAATAAAACCACAC
AAGTGTACTTACTGTGGGAAAGCCTTCACTAGATCAACTCAACTTACTGAACATGTAAGA
ACTCACACTGGAATAAAACCCTATGAATGTAAGGAATGTGGCCAAGCCTTTGCTCAGTAC
TCGGGCCTTTCTATACACATACGAAGTCACAGTGGAAAGAAACCCTATCAGTGTAAGGAA
TGTGGGAAAGCCTTCACTACATCCACAAGTCTTATTCAGCATACAAGAATTCACACAGGA
GAGAAGCCTTATGAATGTGTTGAATGTGGGAAGACCTTCATTACTTCTTCCCGTCGTAGT
AAACATTTGAAAACTCATAGTGGAGAAAAGCCCTTTGTATGCAAGATATGTGGGAAAGCA
TTTCTATATTCCTCACGCCTTAATGTTCACCTGCGAACTCATACCGGAGAGAAACCCTTC
GTATGTAAAGAATGTGGGAAAGCATTTGCTGTTTCCTCACGCCTAAGTAGACATGAAAGA
ATTCACACTGGGGAGAAACCCTATGAATGCAAGGATATGAGTGTTACCATTTTG


Coding Sequence Details
Insert Sequence Verified?: N Verification Method: Insert was fully sequenced in parent vector
Reference Sequence Annotations:
End on reference sequence 1658
Start on reference sequence 405
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
5p GTACAAAAAAGCAGGCTCCACC(atg-goi)

3' Linker Sequence:
5p (goi-stop)GACCCAGCTTTCTTGTAC

 

 Recommended Growth Condition:

Distributed in bacterial strain : DH5-alpha T1 phage resistant
Antibiotic Selection: Host Type:  bacterial    Marker:  ampicillin
Host Type:  mammalian    Marker:  blasticidin
Bacterial Selection Condition:  100 ug/mL ampicillin
Growth Condition:  Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:  Commonly used conditions for ampicillin resistant plasmid clones.
Protein Expression Results: None
Recommended expression in: Not Applicable
       DyNA Vector Map

         Powered by LabGenius

Vector Name: pLenti6.2/V5-DEST

pLenti6.2V5-DEST in advanced viewed

Synonyms: pLenti6.2/V5-DEST
Sequencing Primer: Forward:  CMV forward
Reverse:  V5 reverse
Description: Lentiviral mammalian expression vector;CMV promoter and C-terminal V5 tag; ampicillin resistance in bacteria; blasticidin resistance in mammalian cells; Gateway recombinational cloning.
Comments: None
Size (bp): 8813
Parent Vector: None
Empty Vector: None
Properties: Gateway, acceptor (destination), lentiviral infection, mammalian expression, recombinational cloning, with tag/fusion/marker
Author Name: Invitrogen
Publications: None
Vector Map:         Vector Sequence:
Sequence:

 



Vector Features:

Type Name Description Start Position End Position
bacterial origin pUC ori pUC origin of replication 7733 8406
death cassette ccdB ccdB death cassette (removed when insert is present) 3767 3976
packaging signal HIV-1 psi HIV-1 psi packaging signal 521 565
poly-A signal SV40 SV40 polyadenylation signal 5639 5770
promoter RSV RSV/5' LTR hybrid promoter 1 410
promoter CMV CMV promoter driving gene of interest 1809 2392
promoter PGK PGK promoter to drive selection marker in mammalian cells 4266 4771
recombination site attR2 attR2 recombinational cloning site 4127 4142
recombination site attR1 attR1 recombinational cloning site 2440 2456
response element HIV-1 RRE HIV-1 Rev response element (RRE) 1075 1308
selectable marker AmpR Ampicillin resistance gene for selection in bacteria 6728 7588
selectable marker BlastR Blasticidin resistance gene in mammalian cells 4849 5249
selectable marker CmR chloramphenicol resistance marker (removed in plasmids with insert) 2673 3329
splice acceptor site 3' splice acceptor two 3' splice acceptors at 1656 and 1684 1656 1656
splice donor site 5' splice donor 5' splice donor 520 520
tag V5 C-terminal V5 epitope tag 4197 4238

 

  • Gene
  • Pathways
  • Protein
  • Clone
  • Organism
  • Genome
  • Reagents

Gene

 

Gene Symbol : ZNF561
Symbol Nomenclature : ZNF561
Full Nomenclature : zinc finger protein 561
GENEID : 93134
HGNC : 28684
Vega : OTTHUMG00000157054
Target GenBank: BC032668

 

Reference Sequence Alignment

  

HsCD00329655        ------------------------------------------------------------
NM_152289.2         ATGGCAGCCATTTATTTGTCCCGTGGGTTTTTTTCCAGGGAACCAATCTGCCCTTTTGAA
XM_005260151.1      ------------------------------------------------------------
XM_005260150.1      ATGGCAGCCATTTATTTGTCCCGTGGGTTTTTTTCCAGGGAACCAATCTGCCCTTTTGAA
                                                                                

HsCD00329655        ------------------------------------------------------------
NM_152289.2         GAAAAGACAAAGGTAGAAAGGATGGTGGAGGACTACCTGGCAAGTGGTTATCAGGATTCA
XM_005260151.1      ------------------------------------------------------------
XM_005260150.1      GAAAAGACAAAGGTAGAAAGGATGGTGGAGGACTACCTGGCAAGTGGTTATCAGGATTCA
                                                                                

HsCD00329655        ------------------------------------------------------------
NM_152289.2         GTGACGTTTGATGATGTGGCTGTGGACTTCACCCCAGAGGAGTGGGCTTTACTGGACACA
XM_005260151.1      ------------------------------------------------------------
XM_005260150.1      GTGACGTTTGATGATGTGGCTGTGGACTTCACCCCAGAGGAGTGGGCTTTACTGGACACA
                                                                                

HsCD00329655        ---------------------------ATGCTGGAGAACTACATGAACCTGGCCTCTGTG
NM_152289.2         ACTGAGAAATACCTCTACAGAGATGTGATGCTGGAGAACTACATGAACCTGGCCTCTGTG
XM_005260151.1      ------------------------------------------------------------
XM_005260150.1      ACTGAGAAATACCTCTACAGAGATGTGATGCTGGAGAACTACATGAACCTGGCCTCTGTG
                                                                                

HsCD00329655        GAATGGGAAATACAACCTAGAACCAAACGGTCATCACTTCAGCAGGGTTTTTTGAAGAAT
NM_152289.2         GAATGGGAAATACAACCTAGAACCAAACGGTCATCACTTCAGCAGGGTTTTTTGAAGAAT
XM_005260151.1      ------------------------------------------------------------
XM_005260150.1      GAATGGGAAATACAACCTAGAACCAAACGGTCATCACTTCAGCAGGGTTTTTTGAAGAAT
                                                                                

HsCD00329655        CAAATATTCAGTGGGATACAAATGACAAGAGGCTACAGTGGATGGAAACTCTGTGACTGT
NM_152289.2         CAAATATTCAGTGGGATACAAATGACAAGAGGCTACAGTGGATGGAAACTCTGTGACTGT
XM_005260151.1      ---------------------ATGACAAGAGGCTACAGTGGATGGAAACTCTGTGACTGT
XM_005260150.1      CAAATATTCAGTGGGATACAAATGACAAGAGGCTACAGTGGATGGAAACTCTGTGACTGT
                                         ***************************************

HsCD00329655        AAGAATTGTGGAGAGGTCTTCAGGGAACAGTTTTGCCTTAAGACACACATGAGAGTTCAG
NM_152289.2         AAGAATTGTGGAGAGGTCTTCAGGGAACAGTTTTGCCTTAAGACACACATGAGAGTTCAG
XM_005260151.1      AAGAATTGTGGAGAGGTCTTCAGGGAACAGTTTTGCCTTAAGACACACATGAGAGTTCAG
XM_005260150.1      AAGAATTGTGGAGAGGTCTTCAGGGAACAGTTTTGCCTTAAGACACACATGAGAGTTCAG
                    ************************************************************

HsCD00329655        AATGGAGGGAATACTTCTGAGGGTAATTGTTATGGAAAAGACACCCTCAGTGTGCACAAG
NM_152289.2         AATGGAGGGAATACTTCTGAGGGTAATTGTTATGGAAAAGACACCCTCAGTGTGCACAAG
XM_005260151.1      AATGGAGGGAATACTTCTGAGGGTAATTGTTATGGAAAAGACACCCTCAGTGTGCACAAG
XM_005260150.1      AATGGAGGGAATACTTCTGAGGGTAATTGTTATGGAAAAGACACCCTCAGTGTGCACAAG
                    ************************************************************

HsCD00329655        GAAGCCTCTACTGGACAGGAACTTTCCAAATTTAATCCATGTGGAAAAGTCTTTACTCTA
NM_152289.2         GAAGCCTCTACTGGACAGGAACTTTCCAAATTTAATCCATGTGGAAAAGTCTTTACTCTA
XM_005260151.1      GAAGCCTCTACTGGACAGGAACTTTCCAAATTTAATCCATGTGGAAAAGTCTTTACTCTA
XM_005260150.1      GAAGCCTCTACTGGACAGGAACTTTCCAAATTTAATCCATGTGGAAAAGTCTTTACTCTA
                    ************************************************************

HsCD00329655        ACTCCAGGTCTTGCTGTACATCTTGAAGTTCTCAATGCAAGACAACCCTACAAATGTAAG
NM_152289.2         ACTCCAGGTCTTGCTGTACATCTTGAAGTTCTCAATGCAAGACAACCCTACAAATGTAAG
XM_005260151.1      ACTCCAGGTCTTGCTGTACATCTTGAAGTTCTCAATGCAAGACAACCCTACAAATGTAAG
XM_005260150.1      ACTCCAGGTCTTGCTGTACATCTTGAAGTTCTCAATGCAAGACAACCCTACAAATGTAAG
                    ************************************************************

HsCD00329655        GAATGTGGAAAAGGCTTTAAGTATTTTGCAAGCCTTGATAATCATATGGGAATCCACACT
NM_152289.2         GAATGTGGAAAAGGCTTTAAGTATTTTGCAAGCCTTGATAATCATATGGGAATCCACACT
XM_005260151.1      GAATGTGGAAAAGGCTTTAAGTATTTTGCAAGCCTTGATAATCATATGGGAATCCACACT
XM_005260150.1      GAATGTGGAAAAGGCTTTAAGTATTTTGCAAGCCTTGATAATCATATGGGAATCCACACT
                    ************************************************************

HsCD00329655        GATGAGAAACTCTGTGAATTTCAGGAATATGGGAGAGCTGTCACAGCTTCTTCACACCTA
NM_152289.2         GATGAGAAACTCTGTGAATTTCAGGAATATGGGAGAGCTGTCACAGCTTCTTCACACCTA
XM_005260151.1      GATGAGAAACTCTGTGAATTTCAGGAATATGGGAGAGCTGTCACAGCTTCTTCACACCTA
XM_005260150.1      GATGAGAAACTCTGTGAATTTCAGGAATATGGGAGAGCTGTCACAGCTTCTTCACACCTA
                    ************************************************************

HsCD00329655        AAGCAGTGTGTAGCAGTTCATACAGGAAAGAAATCCAAAAAGACTAAGAAATGTGGGAAA
NM_152289.2         AAGCAGTGTGTAGCAGTTCATACAGGAAAGAAATCCAAAAAGACTAAGAAATGTGGGAAA
XM_005260151.1      AAGCAGTGTGTAGCAGTTCATACAGGAAAGAAATCCAAAAAGACTAAGAAATGTGGGAAA
XM_005260150.1      AAGCAGTGTGTAGCAGTTCATACAGGAAAGAAATCCAAAAAGACTAAGAAATGTGGGAAA
                    ************************************************************

HsCD00329655        TCCTTCACTAATTTTTCTCAACTTTATGCACCTGTGAAAACTCATAAAGGAGAGAAGTCC
NM_152289.2         TCCTTCACTAATTTTTCTCAACTTTATGCACCTGTGAAAACTCATAAAGGAGAGAAGTCC
XM_005260151.1      TCCTTCACTAATTTTTCTCAACTTTATGCACCTGTGAAAACTCATAAAGGAGAGAAGTCC
XM_005260150.1      TCCTTCACTAATTTTTCTCAACTTTATGCACCTGTGAAAACTCATAAAGGAGAGAAGTCC
                    ************************************************************

HsCD00329655        TTTGAATGTAAAGAATGTGGAAGATCCTTTAGAAATTCCTCATGCCTTAATGATCACATT
NM_152289.2         TTTGAATGTAAAGAATGTGGAAGATCCTTTAGAAATTCCTCATGCCTTAATGATCACATT
XM_005260151.1      TTTGAATGTAAAGAATGTGGAAGATCCTTTAGAAATTCCTCATGCCTTAATGATCACATT
XM_005260150.1      TTTGAATGTAAAGAATGTGGAAGATCCTTTAGAAATTCCTCATGCCTTAATGATCACATT
                    ************************************************************

HsCD00329655        CAAATTCACACTGGAATAAAACCACACAAGTGTACTTACTGTGGGAAAGCCTTCACTAGA
NM_152289.2         CAAATTCACACTGGAATAAAACCACACAAGTGTACTTACTGTGGGAAAGCCTTCACTAGA
XM_005260151.1      CAAATTCACACTGGAATAAAACCACACAAGTGTACTTACTGTGGGAAAGCCTTCACTAGA
XM_005260150.1      CAAATTCACACTGGAATAAAACCACACAAGTGTACTTACTGTGGGAAAGCCTTCACTAGA
                    ************************************************************

HsCD00329655        TCAACTCAACTTACTGAACATGTAAGAACTCACACTGGAATAAAACCCTATGAATGTAAG
NM_152289.2         TCAACTCAACTTACTGAACATGTAAGAACTCACACTGGAATAAAACCCTATGAATGTAAG
XM_005260151.1      TCAACTCAACTTACTGAACATGTAAGAACTCACACTGGAATAAAACCCTATGAATGTAAG
XM_005260150.1      TCAACTCAACTTACTGAACATGTAAGAACTCACACTGGAATAAAACCCTATGAATGTAAG
                    ************************************************************

HsCD00329655        GAATGTGGCCAAGCCTTTGCTCAGTACTCGGGCCTTTCTATACACATACGAAGTCACAGT
NM_152289.2         GAATGTGGCCAAGCCTTTGCTCAGTACTCGGGCCTTTCTATACACATACGAAGTCACAGT
XM_005260151.1      GAATGTGGCCAAGCCTTTGCTCAGTACTCGGGCCTTTCTATACACATACGAAGTCACAGT
XM_005260150.1      GAATGTGGCCAAGCCTTTGCTCAGTACTCGGGCCTTTCTATACACATACGAAGTCACAGT
                    ************************************************************

HsCD00329655        GGAAAGAAACCCTATCAGTGTAAGGAATGTGGGAAAGCCTTCACTACATCCACAAGTCTT
NM_152289.2         GGAAAGAAACCCTATCAGTGTAAGGAATGTGGGAAAGCCTTCACTACATCCACAAGTCTT
XM_005260151.1      GGAAAGAAACCCTATCAGTGTAAGGAATGTGGGAAAGCCTTCACTACATCCACAAGTCTT
XM_005260150.1      GGAAAGAAACCCTATCAGTGTAAGGAATGTGGGAAAGCCTTCACTACATCCACAAGTCTT
                    ************************************************************

HsCD00329655        ATTCAGCATACAAGAATTCACACAGGAGAGAAGCCTTATGAATGTGTTGAATGTGGGAAG
NM_152289.2         ATTCAGCATACAAGAATTCACACAGGAGAGAAGCCTTATGAATGTGTTGAATGTGGGAAG
XM_005260151.1      ATTCAGCATACAAGAATTCACACAGGAGAGAAGCCTTATGAATGTGTTGAATGTGGGAAG
XM_005260150.1      ATTCAGCATACAAGAATTCACACAGGAGAGAAGCCTTATGAATGTGTTGAATGTGGGAAG
                    ************************************************************

HsCD00329655        ACCTTCATTACTTCTTCCCGTCGTAGTAAACATTTGAAAACTCATAGTGGAGAAAAGCCC
NM_152289.2         ACCTTCATTACTTCTTCCCGTCGTAGTAAACATTTGAAAACTCATAGTGGAGAAAAGCCC
XM_005260151.1      ACCTTCATTACTTCTTCCCGTCGTAGTAAACATTTGAAAACTCATAGTGGAGAAAAGCCC
XM_005260150.1      ACCTTCATTACTTCTTCCCGTCGTAGTAAACATTTGAAAACTCATAGTGGAGAAAAGCCC
                    ************************************************************

HsCD00329655        TTTGTATGCAAGATATGTGGGAAAGCATTTCTATATTCCTCACGCCTTAATGTTCACCTG
NM_152289.2         TTTGTATGCAAGATATGTGGGAAAGCATTTCTATATTCCTCACGCCTTAATGTTCACCTG
XM_005260151.1      TTTGTATGCAAGATATGTGGGAAAGCATTTCTATATTCCTCACGCCTTAATGTTCACCTG
XM_005260150.1      TTTGTATGCAAGATATGTGGGAAAGCATTTCTATATTCCTCACGCCTTAATGTTCACCTG
                    ************************************************************

HsCD00329655        CGAACTCATACCGGAGAGAAACCCTTCGTATGTAAAGAATGTGGGAAAGCATTTGCTGTT
NM_152289.2         CGAACTCATACCGGAGAGAAACCCTTCGTATGTAAAGAATGTGGGAAAGCATTTGCTGTT
XM_005260151.1      CGAACTCATACCGGAGAGAAACCCTTCGTATGTAAAGAATGTGGGAAAGCATTTGCTGTT
XM_005260150.1      CGAACTCATACCGGAGAGAAACCCTTCGTATGTAAAGAATGTGGGAAAGCATTTGCTGTT
                    ************************************************************

HsCD00329655        TCCTCACGCCTAAGTAGACATGAAAGAATTCACACTGGGGAGAAACCCTATGAATGCAAG
NM_152289.2         TCCTCACGCCTAAGTAGACATGAAAGAATTCACACTGGGGAGAAACCCTATGAATGCAAG
XM_005260151.1      TCCTCACGCCTAAGTAGACATGAAAGAATTCACACTGGGGAGAAACCCTATGAATGCAAG
XM_005260150.1      TCCTCACGCCTAAGTAGACATGAAAGAATTCACACTGGGGAGAAACCCTATGAATGCAAG
                    ************************************************************

HsCD00329655        GATATGAGTGTTACCATTTTG
NM_152289.2         GATATGAGTGTTACCATTTAG
XM_005260151.1      GATATGAGTGTTACCATTTAG
XM_005260150.1      GATATGAGTGTTACCATTTAG
                    *******************:*

Pathways

Reactome : Gene Expression
Reactome : Generic Transcription Pathway

Protein

SMART domain : ZnF_C2H2 : zinc finger
UniProt : Q8N587
HPRD : 15843

Clone

Cloning Information : 268105
HIP Master Clone ID : 193139
Original Clone ID : FLH268105.01L

Organism


TAX_ID : 9606
Species Specific ID: 93134

Genome

Chromosome : 19
Map Location : 19p13.2
Ensembl : ENSG00000171469

Reagents

Labome : ZNF561-antibody