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HTLF (Homo sapiens) in pLenti6.2/V5-DEST (V5-tagged lentiviral mammalian expression vector)

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Explanation of Terms


Gene: Gene Symbol:  FOXN2
Gene Name:  forkhead box N2
Sequence             
Original Clone ID: FLH268109.01L
Keyword: None
Species: Homo sapiens
Type: cDNA
Vector Name: pLenti6.2/V5-DEST               Format:  FUSION
Source: HIP
Description: None
Comments: None
Mutation/
Discrepancy :
No / No
Publications: None
Authors:
HsCD00329658

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AVAILABLE
No restriction
Special MTA: None

 

Insert sequence: 1296nts         Open reading frame : 1 to 1296
Reference Sequence Alignment

ATGGGTCCAGTAATTGGAATGACTCCAGATAAGAGAGCTGAAACCCCAGGAGCTGAAAAG
ATTGCAGGATTAAGCCAGATTTACAAAATGGGAAGCTTGCCTGAAGCTGTTGATGCTGCC
AGGCCGAAGGCCACTCTAGTGGACAGTGAGTCAGCAGATGATGAACTCACAAACTTGAAC
TGGCTTCATGAAAGCACTAATCTTCTAACAAACTTCAGCCTCGGAAGTGAGGGTCTTCCA
ATTGTTAGTCCATTGTATGACATAGAGGGAGATGATGTGCCATCCTTTGGACCAGCTTGC
TACCAGAACCCAGAAAAAAAATCAGCGACTTCAAAGCCCCCATACTCCTTTAGTCTTCTC
ATTTATATGGCCATTGAGCACTCTCCAAATAAATGTTTGCCTGTCAAAGAAATTTATAGC
TGGATTCTGGACCATTTTCCATATTTTGCTACTGCACCAACAGGCTGGAAGAATTCTGTT
CGACATAATCTGTCCCTGAATAAATGTTTTCAGAAAGTGGAAAGAAGCCATGGCAAGGTT
AATGGAAAAGGTTCCTTATGGTGTGTTGATCCGGAATATAAACCCAATCTTATCCAGGCA
CTGAAGAAGCAACCTTTTTCTTCAGCATCTTCACAAAATGGTTCTTTATCACCTCACTAT
TTAAGCTCTGTAATCAAGCAGAACCAGGTGCGAAACCTCAAAGAATCTGATATTGATGCT
GCTGCTGCAATGATGCTTTTAAATACTTCTATAGAACAAGGAATTTTAGAATGTGAGAAG
CCTCTTCCTCTTAAAACAGCATTGCAAAAAAAGAGGAGTTACGGCAATGCATTTCATCAT
CCCAGTGCTGTACGATTACAAGAGAGTGATTCTTTAGCCACCAGCATTGATCCAAAAGAA
GATCACAATTACAGTGCAAGTAGCATGGCAGCACAGCGTTGTGCATCCAGGTCTAGCGTG
TCTTCTCTGTCTTCTGTGGATGAGGTATATGAATTTATCCCAAAGAATAGTCACGTGGGA
AGTGATGGCAGTGAAGGATTTCACAGTGAAGAAGATACAGACGTTGATTATGAAGATGAT
CCTCTTGGAGACAGTGGCTATGCATCACAGCCTTGTGCAAAAATCTCTGAAAAAGGGCAG
TCAGGCAAAAAGATGCGAAAACAGACATGTCAAGAAATTGATGAGGAGCTCAAAGAGGCA
GCTGGATCTCTGCTCCACCTTGCTGGAATTCGTACATGTTTAGGTTCCCTAATAAGTACT
GCAAAGACACAAAATCAAAAGCAACGGAAAAAATTG


Coding Sequence Details
Insert Sequence Verified?: N Verification Method: Insert was fully sequenced in parent vector
Reference Sequence Annotations:
End on reference sequence 1536
Start on reference sequence 241
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
5p GTACAAAAAAGCAGGCTCCACC(atg-goi)

3' Linker Sequence:
5p (goi-stop)GACCCAGCTTTCTTGTAC

 

 Recommended Growth Condition:

Distributed in bacterial strain : DH5-alpha T1 phage resistant
Antibiotic Selection: Host Type:  bacterial    Marker:  ampicillin
Host Type:  mammalian    Marker:  blasticidin
Bacterial Selection Condition:  100 ug/mL ampicillin
Growth Condition:  Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:  Commonly used conditions for ampicillin resistant plasmid clones.
Protein Expression Results: None
Recommended expression in: Not Applicable
       DyNA Vector Map

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Vector Name: pLenti6.2/V5-DEST

pLenti6.2V5-DEST in advanced viewed

Synonyms: pLenti6.2/V5-DEST
Sequencing Primer: Forward:  CMV forward
Reverse:  V5 reverse
Description: Lentiviral mammalian expression vector;CMV promoter and C-terminal V5 tag; ampicillin resistance in bacteria; blasticidin resistance in mammalian cells; Gateway recombinational cloning.
Comments: None
Size (bp): 8813
Parent Vector: None
Empty Vector: None
Properties: Gateway, acceptor (destination), lentiviral infection, mammalian expression, recombinational cloning, with tag/fusion/marker
Author Name: Invitrogen
Publications: None
Vector Map:         Vector Sequence:
Sequence:

 



Vector Features:

Type Name Description Start Position End Position
bacterial origin pUC ori pUC origin of replication 7733 8406
death cassette ccdB ccdB death cassette (removed when insert is present) 3767 3976
packaging signal HIV-1 psi HIV-1 psi packaging signal 521 565
poly-A signal SV40 SV40 polyadenylation signal 5639 5770
promoter RSV RSV/5' LTR hybrid promoter 1 410
promoter CMV CMV promoter driving gene of interest 1809 2392
promoter PGK PGK promoter to drive selection marker in mammalian cells 4266 4771
recombination site attR2 attR2 recombinational cloning site 4127 4142
recombination site attR1 attR1 recombinational cloning site 2440 2456
response element HIV-1 RRE HIV-1 Rev response element (RRE) 1075 1308
selectable marker AmpR Ampicillin resistance gene for selection in bacteria 6728 7588
selectable marker BlastR Blasticidin resistance gene in mammalian cells 4849 5249
selectable marker CmR chloramphenicol resistance marker (removed in plasmids with insert) 2673 3329
splice acceptor site 3' splice acceptor two 3' splice acceptors at 1656 and 1684 1656 1656
splice donor site 5' splice donor 5' splice donor 520 520
tag V5 C-terminal V5 epitope tag 4197 4238

 

  • Gene
  • Protein
  • Clone
  • Organism
  • Genome
  • Reagents

Gene

 

Gene Symbol : FOXN2
Symbol Nomenclature : FOXN2
SYNONYM : HTLF
Designation : forkhead box protein N2|human T-cell leukemia virus enhancer factor
Full Nomenclature : forkhead box N2
GENEID : 3344
HGNC : 5281
MIM : 143089
Vega : OTTHUMG00000129168
Target GenBank: BC063305

 

Reference Sequence Alignment

  

HsCD00329658        ATGGGTCCAGTAATTGGAATGACTCCAGATAAGAGAGCTGAAACCCCAGGAGCTGAAAAG
XM_005264283.1      ATGGGTCCAGTAATTGGAATGACTCCAGATAAGAGAGCTGAAACCCCAGGAGCTGAAAAG
XM_005264284.1      ATGGGTCCAGTAATTGGAATGACTCCAGATAAGAGAGCTGAAACCCCAGGAGCTGAAAAG
XM_005264282.1      ATGGGTCCAGTAATTGGAATGACTCCAGATAAGAGAGCTGAAACCCCAGGAGCTGAAAAG
NM_002158.3         ATGGGTCCAGTAATTGGAATGACTCCAGATAAGAGAGCTGAAACCCCAGGAGCTGAAAAG
                    ************************************************************

HsCD00329658        ATTGCAGGATTAAGCCAGATTTACAAAATGGGAAGCTTGCCTGAAGCTGTTGATGCTGCC
XM_005264283.1      ATTGCAGGATTAAGCCAGATTTACAAAATGGGAAGCTTGCCTGAAGCTGTTGATGCTGCC
XM_005264284.1      ATTGCAGGATTAAGCCAGATTTACAAAATGGGAAGCTTGCCTGAAGCTGTTGATGCTGCC
XM_005264282.1      ATTGCAGGATTAAGCCAGATTTACAAAATGGGAAGCTTGCCTGAAGCTGTTGATGCTGCC
NM_002158.3         ATTGCAGGATTAAGCCAGATTTACAAAATGGGAAGCTTGCCTGAAGCTGTTGATGCTGCC
                    ************************************************************

HsCD00329658        AGGCCGAAGGCCACTCTAGTGGACAGTGAGTCAGCAGATGATGAACTCACAAACTTGAAC
XM_005264283.1      AGGCCGAAGGCCACTCTAGTGGACAGTGAGTCAGCAGATGATGAACTCACAAACTTGAAC
XM_005264284.1      AGGCCGAAGGCCACTCTAGTGGACAGTGAGTCAGCAGATGATGAACTCACAAACTTGAAC
XM_005264282.1      AGGCCGAAGGCCACTCTAGTGGACAGTGAGTCAGCAGATGATGAACTCACAAACTTGAAC
NM_002158.3         AGGCCGAAGGCCACTCTAGTGGACAGTGAGTCAGCAGATGATGAACTCACAAACTTGAAC
                    ************************************************************

HsCD00329658        TGGCTTCATGAAAGCACTAATCTTCTAACAAACTTCAGCCTCGGAAGTGAGGGTCTTCCA
XM_005264283.1      TGGCTTCATGAAAGCACTAATCTTCTAACAAACTTCAGCCTCGGAAGTGAGGGTCTTCCA
XM_005264284.1      TGGCTTCATGAAAGCACTAATCTTCTAACAAACTTCAGCCTCGGAAGTGAGGGTCTTCCA
XM_005264282.1      TGGCTTCATGAAAGCACTAATCTTCTAACAAACTTCAGCCTCGGAAGTGAGGGTCTTCCA
NM_002158.3         TGGCTTCATGAAAGCACTAATCTTCTAACAAACTTCAGCCTCGGAAGTGAGGGTCTTCCA
                    ************************************************************

HsCD00329658        ATTGTTAGTCCATTGTATGACATAGAGGGAGATGATGTGCCATCCTTTGGACCAGCTTGC
XM_005264283.1      ATTGTTAGTCCATTGTATGACATAGAGGGAGATGATGTGCCATCCTTTGGACCAGCTTGC
XM_005264284.1      ATTGTTAGTCCATTGTATGACATAGAGGGAGATGATGTGCCATCCTTTGGACCAGCTTGC
XM_005264282.1      ATTGTTAGTCCATTGTATGACATAGAGGGAGATGATGTGCCATCCTTTGGACCAGCTTGC
NM_002158.3         ATTGTTAGTCCATTGTATGACATAGAGGGAGATGATGTGCCATCCTTTGGACCAGCTTGC
                    ************************************************************

HsCD00329658        TACCAGAACCCAGAAAAAAAATCAGCGACTTCAAAGCCCCCATACTCCTTTAGTCTTCTC
XM_005264283.1      TACCAGAACCCAGAAAAAAAATCAGCGACTTCAAAGCCCCCATACTCCTTTAGTCTTCTC
XM_005264284.1      TACCAGAACCCAGAAAAAAAATCAGCGACTTCAAAGCCCCCATACTCCTTTAGTCTTCTC
XM_005264282.1      TACCAGAACCCAGAAAAAAAATCAGCGACTTCAAAGCCCCCATACTCCTTTAGTCTTCTC
NM_002158.3         TACCAGAACCCAGAAAAAAAATCAGCGACTTCAAAGCCCCCATACTCCTTTAGTCTTCTC
                    ************************************************************

HsCD00329658        ATTTATATGGCCATTGAGCACTCTCCAAATAAATGTTTGCCTGTCAAAGAAATTTATAGC
XM_005264283.1      ATTTATATGGCCATTGAGCACTCTCCAAATAAATGTTTGCCTGTCAAAGAAATTTATAGC
XM_005264284.1      ATTTATATGGCCATTGAGCACTCTCCAAATAAATGTTTGCCTGTCAAAGAAATTTATAGC
XM_005264282.1      ATTTATATGGCCATTGAGCACTCTCCAAATAAATGTTTGCCTGTCAAAGAAATTTATAGC
NM_002158.3         ATTTATATGGCCATTGAGCACTCTCCAAATAAATGTTTGCCTGTCAAAGAAATTTATAGC
                    ************************************************************

HsCD00329658        TGGATTCTGGACCATTTTCCATATTTTGCTACTGCACCAACAGGCTGGAAGAATTCTGTT
XM_005264283.1      TGGATTCTGGACCATTTTCCATATTTTGCTACTGCACCAACAGGCTGGAAGAATTCTGTT
XM_005264284.1      TGGATTCTGGACCATTTTCCATATTTTGCTACTGCACCAACAGGCTGGAAGAATTCTGTT
XM_005264282.1      TGGATTCTGGACCATTTTCCATATTTTGCTACTGCACCAACAGGCTGGAAGAATTCTGTT
NM_002158.3         TGGATTCTGGACCATTTTCCATATTTTGCTACTGCACCAACAGGCTGGAAGAATTCTGTT
                    ************************************************************

HsCD00329658        CGACATAATCTGTCCCTGAATAAATGTTTTCAGAAAGTGGAAAGAAGCCATGGCAAGGTT
XM_005264283.1      CGACATAATCTGTCCCTGAATAAATGTTTTCAGAAAGTGGAAAGAAGCCATGGCAAGGTT
XM_005264284.1      CGACATAATCTGTCCCTGAATAAATGTTTTCAGAAAGTGGAAAGAAGCCATGGCAAGGTT
XM_005264282.1      CGACATAATCTGTCCCTGAATAAATGTTTTCAGAAAGTGGAAAGAAGCCATGGCAAGGTT
NM_002158.3         CGACATAATCTGTCCCTGAATAAATGTTTTCAGAAAGTGGAAAGAAGCCATGGCAAGGTT
                    ************************************************************

HsCD00329658        AATGGAAAAGGTTCCTTATGGTGTGTTGATCCGGAATATAAACCCAATCTTATCCAGGCA
XM_005264283.1      AATGGAAAAGGTTCCTTATGGTGTGTTGATCCGGAATATAAACCCAATCTTATCCAGGCA
XM_005264284.1      AATGGAAAAGGTTCCTTATGGTGTGTTGATCCGGAATATAAACCCAATCTTATCCAGGCA
XM_005264282.1      AATGGAAAAGGTTCCTTATGGTGTGTTGATCCGGAATATAAACCCAATCTTATCCAGGCA
NM_002158.3         AATGGAAAAGGTTCCTTATGGTGTGTTGATCCGGAATATAAACCCAATCTTATCCAGGCA
                    ************************************************************

HsCD00329658        CTGAAGAAGCAACCTTTTTCTTCAGCATCTTCACAAAATGGTTCTTTATCACCTCACTAT
XM_005264283.1      CTGAAGAAGCAACCTTTTTCTTCAGCATCTTCACAAAATGGTTCTTTATCACCTCACTAT
XM_005264284.1      CTGAAGAAGCAACCTTTTTCTTCAGCATCTTCACAAAATGGTTCTTTATCACCTCACTAT
XM_005264282.1      CTGAAGAAGCAACCTTTTTCTTCAGCATCTTCACAAAATGGTTCTTTATCACCTCACTAT
NM_002158.3         CTGAAGAAGCAACCTTTTTCTTCAGCATCTTCACAAAATGGTTCTTTATCACCTCACTAT
                    ************************************************************

HsCD00329658        TTAAGCTCTGTAATCAAGCAGAACCAGGTGCGAAACCTCAAAGAATCTGATATTGATGCT
XM_005264283.1      TTAAGCTCTGTAATCAAGCAGAACCAGGTGCGAAACCTCAAAGAATCTGATATTGATGCT
XM_005264284.1      TTAAGCTCTGTAATCAAGCAGAACCAGGTGCGAAACCTCAAAGAATCTGATATTGATGCT
XM_005264282.1      TTAAGCTCTGTAATCAAGCAGAACCAGGTGCGAAACCTCAAAGAATCTGATATTGATGCT
NM_002158.3         TTAAGCTCTGTAATCAAGCAGAACCAGGTGCGAAACCTCAAAGAATCTGATATTGATGCT
                    ************************************************************

HsCD00329658        GCTGCTGCAATGATGCTTTTAAATACTTCTATAGAACAAGGAATTTTAGAATGTGAGAAG
XM_005264283.1      GCTGCTGCAATGATGCTTTTAAATACTTCTATAGAACAAGGAATTTTAGAATGTGAGAAG
XM_005264284.1      GCTGCTGCAATGATGCTTTTAAATACTTCTATAGAACAAGGAATTTTAGAATGTGAGAAG
XM_005264282.1      GCTGCTGCAATGATGCTTTTAAATACTTCTATAGAACAAGGAATTTTAGAATGTGAGAAG
NM_002158.3         GCTGCTGCAATGATGCTTTTAAATACTTCTATAGAACAAGGAATTTTAGAATGTGAGAAG
                    ************************************************************

HsCD00329658        CCTCTTCCTCTTAAAACAGCATTGCAAAAAAAGAGGAGTTACGGCAATGCATTTCATCAT
XM_005264283.1      CCTCTTCCTCTTAAAACAGCATTGCAAAAAAAGAGGAGTTACGGCAATGCATTTCATCAT
XM_005264284.1      CCTCTTCCTCTTAAAACAGCATTGCAAAAAAAGAGGAGTTACGGCAATGCATTTCATCAT
XM_005264282.1      CCTCTTCCTCTTAAAACAGCATTGCAAAAAAAGAGGAGTTACGGCAATGCATTTCATCAT
NM_002158.3         CCTCTTCCTCTTAAAACAGCATTGCAAAAAAAGAGGAGTTACGGCAATGCATTTCATCAT
                    ************************************************************

HsCD00329658        CCCAGTGCTGTACGATTACAAGAGAGTGATTCTTTAGCCACCAGCATTGATCCAAAAGAA
XM_005264283.1      CCCAGTGCTGTACGATTACAAGAGAGTGATTCTTTAGCCACCAGCATTGATCCAAAAGAA
XM_005264284.1      CCCAGTGCTGTACGATTACAAGAGAGTGATTCTTTAGCCACCAGCATTGATCCAAAAGAA
XM_005264282.1      CCCAGTGCTGTACGATTACAAGAGAGTGATTCTTTAGCCACCAGCATTGATCCAAAAGAA
NM_002158.3         CCCAGTGCTGTACGATTACAAGAGAGTGATTCTTTAGCCACCAGCATTGATCCAAAAGAA
                    ************************************************************

HsCD00329658        GATCACAATTACAGTGCAAGTAGCATGGCAGCACAGCGTTGTGCATCCAGGTCTAGCGTG
XM_005264283.1      GATCACAATTACAGTGCAAGTAGCATGGCAGCACAGCGTTGTGCATCCAGGTCTAGCGTG
XM_005264284.1      GATCACAATTACAGTGCAAGTAGCATGGCAGCACAGCGTTGTGCATCCAGGTCTAGCGTG
XM_005264282.1      GATCACAATTACAGTGCAAGTAGCATGGCAGCACAGCGTTGTGCATCCAGGTCTAGCGTG
NM_002158.3         GATCACAATTACAGTGCAAGTAGCATGGCAGCACAGCGTTGTGCATCCAGGTCTAGCGTG
                    ************************************************************

HsCD00329658        TCTTCTCTGTCTTCTGTGGATGAGGTATATGAATTTATCCCAAAGAATAGTCACGTGGGA
XM_005264283.1      TCTTCCCTGTCTTCTGTGGATGAGGTATATGAATTTATCCCAAAGAATAGTCACGTGGGA
XM_005264284.1      TCTTCCCTGTCTTCTGTGGATGAGGTATATGAATTTATCCCAAAGAATAGTCACGTGGGA
XM_005264282.1      TCTTCCCTGTCTTCTGTGGATGAGGTATATGAATTTATCCCAAAGAATAGTCACGTGGGA
NM_002158.3         TCTTCCCTGTCTTCTGTGGATGAGGTATATGAATTTATCCCAAAGAATAGTCACGTGGGA
                    ***** ******************************************************

HsCD00329658        AGTGATGGCAGTGAAGGATTTCACAGTGAAGAAGATACAGACGTTGATTATGAAGATGAT
XM_005264283.1      AGTGATGGCAGTGAAGGATTTCACAGTGAAGAAGATACAGACGTTGATTATGAAGATGAT
XM_005264284.1      AGTGATGGCAGTGAAGGATTTCACAGTGAAGAAGATACAGACGTTGATTATGAAGATGAT
XM_005264282.1      AGTGATGGCAGTGAAGGATTTCACAGTGAAGAAGATACAGACGTTGATTATGAAGATGAT
NM_002158.3         AGTGATGGCAGTGAAGGATTTCACAGTGAAGAAGATACAGACGTTGATTATGAAGATGAT
                    ************************************************************

HsCD00329658        CCTCTTGGAGACAGTGGCTATGCATCACAGCCTTGTGCAAAAATCTCTGAAAAAGGGCAG
XM_005264283.1      CCTCTTGGAGACAGTGGCTATGCATCACAGCCTTGTGCAAAAATCTCTGAAAAAGGGCAG
XM_005264284.1      CCTCTTGGAGACAGTGGCTATGCATCACAGCCTTGTGCAAAAATCTCTGAAAAAGGGCAG
XM_005264282.1      CCTCTTGGAGACAGTGGCTATGCATCACAGCCTTGTGCAAAAATCTCTGAAAAAGGGCAG
NM_002158.3         CCTCTTGGAGACAGTGGCTATGCATCACAGCCTTGTGCAAAAATCTCTGAAAAAGGGCAG
                    ************************************************************

HsCD00329658        TCAGGCAAAAAGATGCGAAAACAGACATGTCAAGAAATTGATGAGGAGCTCAAAGAGGCA
XM_005264283.1      TCAGGCAAAAAGATGCGAAAACAGACATGTCAAGAAATTGATGAGGAGCTCAAAGAGGCA
XM_005264284.1      TCAGGCAAAAAGATGCGAAAACAGACATGTCAAGAAATTGATGAGGAGCTCAAAGAGGCA
XM_005264282.1      TCAGGCAAAAAGATGCGAAAACAGACATGTCAAGAAATTGATGAGGAGCTCAAAGAGGCA
NM_002158.3         TCAGGCAAAAAGATGCGAAAACAGACATGTCAAGAAATTGATGAGGAGCTCAAAGAGGCA
                    ************************************************************

HsCD00329658        GCTGGATCTCTGCTCCACCTTGCTGGAATTCGTACATGTTTAGGTTCCCTAATAAGTACT
XM_005264283.1      GCTGGATCTCTGCTCCACCTTGCTGGAATTCGTACATGTTTAGGTTCCCTAATAAGTACT
XM_005264284.1      GCTGGATCTCTGCTCCACCTTGCTGGAATTCGTACATGTTTAGGTTCCCTAATAAGTACT
XM_005264282.1      GCTGGATCTCTGCTCCACCTTGCTGGAATTCGTACATGTTTAGGTTCCCTAATAAGTACT
NM_002158.3         GCTGGATCTCTGCTCCACCTTGCTGGAATTCGTACATGTTTAGGTTCCCTAATAAGTACT
                    ************************************************************

HsCD00329658        GCAAAGACACAAAATCAAAAGCAACGGAAAAAATTG
XM_005264283.1      GCAAAGACACAAAATCAAAAGCAACGGAAAAAATAG
XM_005264284.1      GCAAAGACACAAAATCAAAAGCAACGGAAAAAATAG
XM_005264282.1      GCAAAGACACAAAATCAAAAGCAACGGAAAAAATAG
NM_002158.3         GCAAAGACACAAAATCAAAAGCAACGGAAAAAATAG
                    **********************************:*

Protein

SMART domain : FH : FORKHEAD
UniProt : P32314
HPRD : 00882

Clone

Cloning Information : 268109
HIP Master Clone ID : 193145
Original Clone ID : FLH268109.01L

Organism


TAX_ID : 9606
Species Specific ID: 3344

Genome

Chromosome : 2
Map Location : 2p22-p16
Ensembl : ENSG00000170802

Reagents

Labome : HTLF-antibody