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TULP3 (Homo sapiens) in pLenti6.2/V5-DEST (V5-tagged lentiviral mammalian expression vector)

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Explanation of Terms


Gene: Gene Symbol:  TULP3
Gene Name:  tubby like protein 3
Sequence             
Original Clone ID: FLH268113.01L
Keyword: None
Species: Homo sapiens
Type: cDNA
Vector Name: pLenti6.2/V5-DEST               Format:  FUSION
Source: HIP
Description: None
Comments: None
Mutation/
Discrepancy :
No / No
Publications: None
Authors:
HsCD00329661

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AVAILABLE
No restriction
Special MTA: None

 

Insert sequence: 1329nts         Open reading frame : 1 to 1329
Reference Sequence Alignment

ATGGAGGCTTCGCGCTGCCGGCTCAGTCCCAGCGGCGACAGTGTCTTCCATGAAGAAATG
ATGAAGATGCGACAGGCTAAGCTGGATTATCAGAGGCTACTACTTGAGAAGAGGCAAAGG
AAAAAGCGCCTTGAGCCATTTATGGTGCAGCCCAATCCAGAAGCCAGGCTACGTCGGGCA
AAGCCAAGGGCCAGTGATGAGCAGACTCCCTTGGTGAACTGTCATACTCCCCACAGCAAT
GTCATCTTACATGGTATTGATGGTCCAGCTGCTGTCCTGAAACCAGACGAAGTTCATGCT
CCATCAGTAAGCTCCTCTGTTGTGGAAGAAGATGCTGAAAACACCGTGGATACTGCTTCC
AAGCCAGGACTTCAGGAGCGTCTCCAAAAGCATGATATCTCTGAAAGTGTGAACTTCGAT
GAGGAGACTGATGGAATATCCCAGTCAGCATGTTTAGAAAGACCCAATTCTGCATCAAGC
CAGAATTCAACCGATACAGGCACTTCCGGTTCTGCTACTGCCGCCCAACCAGCTGATAAC
CTCCTGGGAGACATAGACTACCTGGAGGACTTTGTGTATAGTCCTGCCCCTCAAGGTGTC
ACAGTAAGATGTCGGATAATCCGGGATAAAAGGGGAATGGATCGGGGTCTCTTCCCCACC
TACTATATGTACTTGGAAAAAGAAGAAAATCAGAAGATATTTCTTCTTGCAGCTAGAAAG
CGGAAAAAGAGCAAAACAGCCAACTACCTTATCTCCATTGATCCAGTTGATTTATCTCGT
GAAGGAGAAAGTTATGTCGGCAAGCTTAGATCCAACCTCATGGGGACCAAGTTTACAGTT
TATGACCGTGGCATCTGCCCCATGAAGGGCCGGGGTTTGGTAGGAGCGGCCCACACCCGG
CAGGAGCTGGCTGCCATCTCCTATGAAACAAACGTACTTGGATTTAAAGGTCCTAGGAAA
ATGTCTGTGATCATTCCTGGAATGACACTGAATCATAAGCAGATCCCCTATCAGCCACAA
AACAACCATGACAGTTTGCTCTCAAGGTGGCAGAACAGAACTATGGAAAATCTGGTTGAG
CTGCACAACAAGGCCCCCGTCTGGAACAGTGACACTCAGTCCTATGTCCTCAACTTCCGT
GGCCGGGTCACTCAGGCGTCTGTGAAGAACTTCCAGATAGTCCACAAAAATGACCCTGAT
TATATAGTCATGCAGTTTGGACGTGTGGCAGATGACGTGTTCACACTGGATTACAACTAC
CCACTTTGTGCAGTACAGGCCTTTGGCATCGGTCTTTCTAGCTTTGACAGTAAGCTGGCG
TGTGAATTG


Coding Sequence Details
Insert Sequence Verified?: N Verification Method: Insert was fully sequenced in parent vector
Reference Sequence Annotations:
End on reference sequence 1383
Start on reference sequence 55
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
5p GTACAAAAAAGCAGGCTCCACC(atg-goi)

3' Linker Sequence:
5p (goi-stop)GACCCAGCTTTCTTGTAC

 

 Recommended Growth Condition:

Distributed in bacterial strain : DH5-alpha T1 phage resistant
Antibiotic Selection: Host Type:  bacterial    Marker:  ampicillin
Host Type:  mammalian    Marker:  blasticidin
Bacterial Selection Condition:  100 ug/mL ampicillin
Growth Condition:  Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:  Commonly used conditions for ampicillin resistant plasmid clones.
Protein Expression Results: None
Recommended expression in: Not Applicable
       DyNA Vector Map

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Vector Name: pLenti6.2/V5-DEST

pLenti6.2V5-DEST in advanced viewed

Synonyms: pLenti6.2/V5-DEST
Sequencing Primer: Forward:  CMV forward
Reverse:  V5 reverse
Description: Lentiviral mammalian expression vector;CMV promoter and C-terminal V5 tag; ampicillin resistance in bacteria; blasticidin resistance in mammalian cells; Gateway recombinational cloning.
Comments: None
Size (bp): 8813
Parent Vector: None
Empty Vector: None
Properties: Gateway, acceptor (destination), lentiviral infection, mammalian expression, recombinational cloning, with tag/fusion/marker
Author Name: Invitrogen
Publications: None
Vector Map:         Vector Sequence:
Sequence:

 



Vector Features:

Type Name Description Start Position End Position
bacterial origin pUC ori pUC origin of replication 7733 8406
death cassette ccdB ccdB death cassette (removed when insert is present) 3767 3976
packaging signal HIV-1 psi HIV-1 psi packaging signal 521 565
poly-A signal SV40 SV40 polyadenylation signal 5639 5770
promoter RSV RSV/5' LTR hybrid promoter 1 410
promoter CMV CMV promoter driving gene of interest 1809 2392
promoter PGK PGK promoter to drive selection marker in mammalian cells 4266 4771
recombination site attR2 attR2 recombinational cloning site 4127 4142
recombination site attR1 attR1 recombinational cloning site 2440 2456
response element HIV-1 RRE HIV-1 Rev response element (RRE) 1075 1308
selectable marker AmpR Ampicillin resistance gene for selection in bacteria 6728 7588
selectable marker BlastR Blasticidin resistance gene in mammalian cells 4849 5249
selectable marker CmR chloramphenicol resistance marker (removed in plasmids with insert) 2673 3329
splice acceptor site 3' splice acceptor two 3' splice acceptors at 1656 and 1684 1656 1656
splice donor site 5' splice donor 5' splice donor 520 520
tag V5 C-terminal V5 epitope tag 4197 4238

 

  • Gene
  • Protein
  • Clone
  • Organism
  • Genome
  • Reagents

Gene

 

Gene Symbol : TULP3
Symbol Nomenclature : TULP3
SYNONYM : TUBL3
Designation : tubby-like protein 3|tubby-related protein 3
Full Nomenclature : tubby like protein 3
GENEID : 7289
HGNC : 12425
MIM : 604730
Vega : OTTHUMG00000168152
Target GenBank: BC032587

 

Reference Sequence Alignment

  

HsCD00329661        ATGGAGGCTTCGCGCTGCCGGCTCAGTCCCAGCGGCGACAGTGTCTTCCATGAAGAAATG
NM_003324.4         ATGGAGGCTTCGCGCTGCCGGCTCAGTCCCAGCGGCGACAGTGTCTTCCATGAAGAAATG
NM_001160408.1      ATGGAGGCTTCGCGCTGCCGGCTCAGTCCCAGCGGCGACAGTGTCTTCCATGAAGAAATG
                    ************************************************************

HsCD00329661        ATGAAGATGCGACAGGCTAAGCTGGATTATCAGAGGCTACTACTTGAGAAGAGGCAAAGG
NM_003324.4         ATGAAGATGCGACAGGCTAAGCTGGATTATCAGAGGCTACTACTTGAGAAGAGGCAAAGG
NM_001160408.1      ATGAAGATGCGACAGGCTAAGCTGGATTATCAGAGGCTACTACTTGAGAAGAGGCAAAGG
                    ************************************************************

HsCD00329661        AAAAAGCGCCTTGAGCCATTTATGGTGCAGCCCAATCCAGAAGCCAGGCTACGTCGGGCA
NM_003324.4         AAAAAGCGCCTTGAGCCATTTATGGTGCAGCCCAATCCAGAAGCCAGGCTACGTCGGGCA
NM_001160408.1      AAAAAGCGCCTTGAGCCATTTATGGTGCAGCCCAATCCAGAAGCCAGGCTACGTCGGGCA
                    ************************************************************

HsCD00329661        AAGCCAAGGGCCAGTGATGAGCAGACTCCCTTGGTGAACTGTCATACTCCCCACAGCAAT
NM_003324.4         AAGCCAAGGGCCAGTGATGAGCAGACTCCCTTGGTGAACTGTCATACTCCCCACAGCAAT
NM_001160408.1      AAGCCAAGGGCCAGTGATGAGCAGACTCCCTTGGTGAACTGTCATACTCCCCACAGCAAT
                    ************************************************************

HsCD00329661        GTCATCTTACATGGTATTGATGGTCCAGCTGCTGTCCTGAAACCAGACGAAGTTCATGCT
NM_003324.4         GTCATCTTACATGGTATTGATGGTCCAGCTGCTGTCCTGAAACCAGACGAAGTTCATGCT
NM_001160408.1      GTCATCTTACATGGTATTGATGGTCCAGCTGCTGTCCTGAAACCAGACGAAGTTCATGCT
                    ************************************************************

HsCD00329661        CCATCAGTAAGCTCCTCTGTTGTGGAAGAAGATGCTGAAAACACCGTGGATACTGCTTCC
NM_003324.4         CCATCAGTAAGCTCCTCTGTTGTGGAAGAAGATGCTGAAAACACCGTGGATACTGCTTCC
NM_001160408.1      CCATCAGTAAGCTCCTCTGTTGTGGAAGAAGATGCTGAAAACACCGTGGATACTGCTTCC
                    ************************************************************

HsCD00329661        AAGCCAGGACTTCAGGAGCGTCTCCAAAAGCATGATATCTCTGAAAGTGTGAACTTCGAT
NM_003324.4         AAGCCAGGACTTCAGGAGCGTCTCCAAAAGCATGATATCTCTGAAAGTGTGAACTTCGAT
NM_001160408.1      AAGCCAGGACTTCAGGAGCGTCTCCAAAAGCATGATATCTCTGAAAGTGTGAACTTCGAT
                    ************************************************************

HsCD00329661        GAGGAGACTGATGGAATATCCCAGTCAGCATGTTTAGAAAGACCCAATTCTGCATCAAGC
NM_003324.4         GAGGAGACTGATGGAATATCCCAGTCAGCATGTTTAGAAAGACCCAATTCTGCATCAAGC
NM_001160408.1      GAGGAGACTGATGGAATATCCCAGTCAGCATGTTTAGAAAGACCCAATTCTGCATCAAGC
                    ************************************************************

HsCD00329661        CAGAATTCAACCGATACAGGCACTTCCGGTTCTGCTACTGCCGCCCAACCAGCTGATAAC
NM_003324.4         CAGAATTCAACCGATACAGGCACTTCCGGTTCTGCTACTGCCGCCCAACCAGCTGATAAC
NM_001160408.1      CAGAATTCAACCGATACAGGCACTTCCGGTTCTGCTACTGCCGCCCAACCAGCTGATAAC
                    ************************************************************

HsCD00329661        CTCCTGGGAGACATAGACTACCTGGAGGACTTTGTGTATAGTCCTGCCCCTCAAGGTGTC
NM_003324.4         CTCCTGGGAGACATAGACGACCTGGAGGACTTTGTGTATAGTCCTGCCCCTCAAGGTGTC
NM_001160408.1      CTCCTGGGAGACATAGACGACCTGGAGGACTTTGTGTATAGTCCTGCCCCTCAAGGTGTC
                    ****************** *****************************************

HsCD00329661        ACAGTAAGATGTCGGATAATCCGGGATAAAAGGGGAATGGATCGGGGTCTCTTCCCCACC
NM_003324.4         ACAGTAAGATGTCGGATAATCCGGGATAAAAGGGGAATGGATCGGGGTCTCTTCCCCACC
NM_001160408.1      ACAGTAAGATGTCGGATAATCCGGGATAAAAGGGGAATGGATCGGGGTCTCTTCCCCACC
                    ************************************************************

HsCD00329661        TACTATATGTACTTGGAAAAAGAAGAAAATCAGAAGATATTTCTTCTTGCAGCTAGAAAG
NM_003324.4         TACTATATGTACTTGGAAAAAGAAGAAAATCAGAAGATATTTCTTCTTGCAGCTAGAAAG
NM_001160408.1      TACTATATGTACTTGGAAAAAGAAGAAAATCAGAAGATATTTCTTCTTGCAGCTAGAAAG
                    ************************************************************

HsCD00329661        CGGAAAAAGAGCAAAACAGCCAACTACCTTATCTCCATTGATCCAGTTGATTTATCTCGT
NM_003324.4         CGGAAAAAGAGCAAAACAGCCAACTACCTTATCTCCATTGATCCAGTTGATTTATCTCGT
NM_001160408.1      CGGAAAAAGAGCAAAACAGCCAACTACCTTATCTCCATTGATCCAGTTGATTTATCTCGT
                    ************************************************************

HsCD00329661        GAAGGAGAAAGTTATGTCGGCAAGCTTAGATCCAACCTCATGGGGACCAAGTTTACAGTT
NM_003324.4         GAAGGAGAAAGTTATGTCGGCAAGCTTAGATCCAACCTCATGGGGACCAAGTTTACAGTT
NM_001160408.1      GAAGGAGAAAGTTATGTCGGCAAGCTTAGATCCAACCTCATGGGGACCAAGTTTACAGTT
                    ************************************************************

HsCD00329661        TATGACCGTGGCATCTGCCCCATGAAGGGCCGGGGTTTGGTAGGAGCGGCCCACACCCGG
NM_003324.4         TATGACCGTGGCATCTGCCCCATGAAGGGCCGGGGTTTGGTAGGAGCGGCCCACACCCGG
NM_001160408.1      TATGACCGTGGCATCTGCCCCATGAAGGGCCGGGGTTTGGTAGGAGCGGCCCACACCCGG
                    ************************************************************

HsCD00329661        CAGGAGCTGGCTGCCATCTCCTATGAAACAAACGTACTTGGATTTAAAGGTCCTAGGAAA
NM_003324.4         CAGGAGCTGGCTGCCATCTCCTATGAAACAAACGTACTTGGATTTAAAGGTCCTAGGAAA
NM_001160408.1      CAGGAGCTGGCTGCCATCTCCTATGAAACAAACGTACTTGGATTTAAAGGTCCTAGGAAA
                    ************************************************************

HsCD00329661        ATGTCTGTGATCATTCCTGGAATGACACTGAATCATAAGCAGATCCCCTATCAGCCACAA
NM_003324.4         ATGTCTGTGATCATTCCTGGAATGACACTGAATCATAAGCAGATCCCCTATCAGCCACAA
NM_001160408.1      ATGTCTGTGATCATTCCTGGAATGACACTGAATCATAAGCAGATCCCCTATCAGCCACAA
                    ************************************************************

HsCD00329661        AACAACCATGACAGTTTGCTCTCAAGGTGGCAGAACAGAACTATGGAAAATCTGGTTGAG
NM_003324.4         AACAACCATGACAGTTTGCTCTCAAGGTGGCAGAACAGAACTATGGAAAATCTGGTTGAG
NM_001160408.1      AACAACCATGACAGTTTGCTCTCAAGGTGGCAGAACAGAACTATGGAAAATCTGGTTGAG
                    ************************************************************

HsCD00329661        CTGCACAACAAGGCCCCCGTCTGGAACAGTGACACTCAGTCCTATGTCCTCAACTTCCGT
NM_003324.4         CTGCACAACAAGGCCCCCGTCTGGAACAGTGACACTCAGTCCTATGTCCTCAACTTCCGT
NM_001160408.1      CTGCACAACAAGGCCCCCGTCTGGAACAGTGACACTCAGTCCTATGTCCTCAACTTCCGT
                    ************************************************************

HsCD00329661        GGCCGGGTCACTCAGGCGTCTGTGAAGAACTTCCAGATAGTCCACAAAAATGACCCTGAT
NM_003324.4         GGCCGGGTCACTCAGGCGTCTGTGAAGAACTTCCAGATAGTCCACAAAAATGACCCTGAT
NM_001160408.1      GGCCGGGTCACTCAGGCGTCTGTGAAGAACTTCCAGATAGTCCACAAAAATGACCCTGAT
                    ************************************************************

HsCD00329661        TATATAGTCATGCAGTTTGGACGTGTGGCAGATGACGTGTTCACACTGGATTACAACTAC
NM_003324.4         TATATAGTCATGCAGTTTGGACGTGTGGCAGATGACGTGTTCACACTGGATTACAACTAC
NM_001160408.1      TATATAGTCATGCAGTTTGGACGTGTGGCAGATGACGTGTTCACACTGGATTACAACTAC
                    ************************************************************

HsCD00329661        CCACTTTGTGCAGTACAGGCCTTTGGCATCGGTCTTTCTAGCTTTGACAGTAAGCTGGCG
NM_003324.4         CCACTTTGTGCAGTACAGGCCTTTGGCATCGGTCTTTCTAGCTTTGACAGTAAGCTGGCG
NM_001160408.1      CCACTTTGTGCAGTACAGGCCTTTGGCATCGGTCTTTCTAGCTTTGACAAATGTATCCAA
                    *************************************************.::. .*  ..

HsCD00329661        TGTGAATTG---------------------------------------------------
NM_003324.4         TGTGAATGA---------------------------------------------------
NM_001160408.1      ACCTTGAGAATGCAGGAGCTCTGTGAGCTCCACCGTCAGCACCATTCAGCTGCATCCCTT
                    :   :.: .                                                   

HsCD00329661        ------------------------------------------------------------
NM_003324.4         ------------------------------------------------------------
NM_001160408.1      GTGCACAGAACTGTTTGCCAGCGTTGGGTGGGACACCCGTGGCGGCTGCTCCCTCAGACC
                                                                                

HsCD00329661        ------------------------------------------------------------
NM_003324.4         ------------------------------------------------------------
NM_001160408.1      TCCCTTCTGTGGACTGACCTCTCACCTCCGCCTGTTGTTCCTGCACCACATCAGATAAGC
                                                                                

HsCD00329661        ------
NM_003324.4         ------
NM_001160408.1      ATGTGA
                          

Protein

UniProt : B7Z1E7
UniProt : O75386
HPRD : 05292

Clone

Cloning Information : 268113
HIP Master Clone ID : 193149
Original Clone ID : FLH268113.01L

Organism


TAX_ID : 9606
Species Specific ID: 7289

Genome

Chromosome : 12
Map Location : 12p13.3
Ensembl : ENSG00000078246

Reagents

Labome : TULP3-antibody