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TRIM56 (Homo sapiens) in pLenti6.2/V5-DEST (V5-tagged lentiviral mammalian expression vector)

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Explanation of Terms


Gene: Gene Symbol:  TRIM56
Gene Name:  tripartite motif containing 56
Sequence             
Original Clone ID: FLH268115.01L
Keyword: None
Species: Homo sapiens
Type: cDNA
Vector Name: pLenti6.2/V5-DEST               Format:  FUSION
Source: HIP
Description: None
Comments: None
Mutation/
Discrepancy :
No / No
Publications: None
Authors:
HsCD00329663

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AVAILABLE
No restriction
Special MTA: None

 

Insert sequence: 2268nts         Open reading frame : 1 to 2268
Reference Sequence Alignment

ATGGTTTCCCACGGGTCCTCGCCCTCCCTCCTGGAGGCCCTGAGCAGCGACTTCCTGGCC
TGTAAAATCTGCCTGGAGCAGCTGCGGGCACCCAAGACACTGCCCTGCCTGCATACCTAC
TGCCAAGACTGCCTGGCACAGCTGGCGGATGGCGGCCGCGTCCGCTGCCCCGAGTGCCGC
GAGACAGTGCCTGTGCCGCCCGAGGGTGTGGCCTCCTTCAAGACCAACTTCTTCGTCAAT
GGGCTGCTGGACCTGGTGAAGGCCCGGGCCTGTGGAGACCTGCGTGCCGGGAAGCCAGCC
TGTGCCCTGTGTCCCCTGGTGGGTGGCACCAGCACCGGGGGGCCGGCCACGGCCCGGTGC
CTGGACTGTGCCGATGACTTGTGCCAGGCCTGTGCCGACGGGCACCGCTGCACCCGCCAG
ACCCACACCCACCGCGTGGTGGACCTGGTGGGCTACAGGGCCGGGTGGTATGATGAGGAG
GCCCGGGAGCGCCAAGCGGCCCAGTGTCCCCAGCACCCCGGGGAGGCACTGCGCTTCCTG
TGCCAGCCCTGCTCACAGTTGCTGTGCAGAGAGTGCCGCCTAGACCCCCACCTGGACCAC
CCCTGCCTGCCTCTGGCTGAAGCTGTGCGTGCCCGGAGGCCGGGCCTGGAGGGACTGCTG
GCCGGTGTGGACAATAACCTGGTGGAGCTGGAGGCAGCGCGGAGGGTGGAGAAGGAGGCG
CTAGCCCGGCTGCGGGAGCAGGCGGCCCGGGTGGGGACTCAGGTGGAGGAGGCGGCTGAG
GGCGTCCTCCGGGCCCTGCTGGCCCAGAAGCAGGAGGTGCTGGGGCAGCTACGAGCCCAC
GTGGAGGCTGCCGAAGAAGCTGCTCGGGAGAGGCTGGCGGAGCTTGAGGGCCGGGAGCAG
GTGGCCAGGGCGGCAGCCGCCTTCGCCCGCCGGGTACTCAGCCTGGGGCGAGAGGCCGAG
ATCCTCTCCCTGGAAGGGGCGATCGCACAGCGGCTCAGGCAGCTGCAGGGCTGCCCCTGG
GCACCAGGCCCGGCCCCCTGCCTGCTCCCACAGCTGGAGCTCCATCCTGGGCTCCTGGAC
AAGAACTGCCACCTTCTTCGGCTGTCCTTTGAGGAGCAGCAGCCCCAGAAGGATGGTGGG
AAAGACGGAGCTGGTACCCAGGGAGGTGAGGAGAGCCAGAGCCGGAGGGAGGATGAGCCG
AAGACTGAGAGACAGGGTGGAGTCCAGCCCCAGGCCGGAGATGGAGCCCAGACCCCAAAA
GAGGAAAAAGCCCAGACAACCCGAGAAGAGGGAGCCCAGACCTTGGAGGAGGACAGGGCC
CAGACACCCCACGAGGATGGAGGACCCCAGCCCCACAGGGGTGGCAGACCCAACAAGAAG
AAAAAGTTCAAAGGCAGGCTCAAGTCAATTTCCCGGGAGCCCAGCCCAGCCCTGGGGCCG
AATCTGGACGGCTCTGGCCTCCTCCCCAGACCCATCTTTTACTGCAGTTTCCCCACGCGG
ATGCCTGGAGACAAGCGGTCCCCCCGGATCACCGGGCTCTGTCCCTTCGGTCCCCGGGAG
ATCCTGGTGGCGGATGAGCAGAACCGGGCACTGAAACGCTTCTCCCTCAACGGCGACTAC
AAGGGCACCGTGCCGGTCCCTGAGGGCTGCTCCCCTTGCAGCGTGGCCGCCCTGCAGAGC
GCGGTGGCCTTCTCCGCTAGCGCACGGCTCTATCTCATCAACCCCAACGGCGAAGTGCAG
TGGCGCAGGGCCCTGAGCCTCTCCCAGGCCAGCCACGCGGTGGCGGCACTGCCTAGCGGG
GACCGCGTGGCTGTCAGCGTGGCGGGCCACGTGGAGGTGTACAATATGGAAGGCAGCCTG
GCCACCCGGTTCATTCCTGGAGGCAAGGCCAGCCGGGGCCTGCGGGCGCTGGTGTTTCTG
ACCACCAGCCCCCAGGGGCATTTCGTGGGGTCGGACTGGCAGCAGAATAGTGTGGTAATC
TGTGATGGGCTGGGCCAGGTGGTTGGGGAGTACAAGGGGCCAGGCCTGCATGGCTGCCAG
CCGGGCTCCGTGTCTGTGGATAAGAAGGGCTACATCTTTCTGACCCTTCGAGAAGTCAAC
AAGGTGGTGATCCTGGACCCGAAGGGGTCCCTCCTTGGAGACTTCCTGACAGCCTACCAC
GGCCTGGAAAAGCCCCGGGTTACCACCATGGTGGATGGCAGGTACCTGGTCGTGTCCCTC
AGTAACGGGACCATCCACATCTTTCGGGTCCGTTCTCCGGACAGTTTG


Coding Sequence Details
Insert Sequence Verified?: N Verification Method: Insert was fully sequenced in parent vector
Reference Sequence Annotations:
End on reference sequence 2499
Start on reference sequence 232
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
5p GTACAAAAAAGCAGGCTCCACC(atg-goi)

3' Linker Sequence:
5p (goi-stop)GACCCAGCTTTCTTGTAC

 

 Recommended Growth Condition:

Distributed in bacterial strain : DH5-alpha T1 phage resistant
Antibiotic Selection: Host Type:  bacterial    Marker:  ampicillin
Host Type:  mammalian    Marker:  blasticidin
Bacterial Selection Condition:  100 ug/mL ampicillin
Growth Condition:  Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:  Commonly used conditions for ampicillin resistant plasmid clones.
Protein Expression Results: None
Recommended expression in: Not Applicable
       DyNA Vector Map

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Vector Name: pLenti6.2/V5-DEST

pLenti6.2V5-DEST in advanced viewed

Synonyms: pLenti6.2/V5-DEST
Sequencing Primer: Forward:  CMV forward
Reverse:  V5 reverse
Description: Lentiviral mammalian expression vector;CMV promoter and C-terminal V5 tag; ampicillin resistance in bacteria; blasticidin resistance in mammalian cells; Gateway recombinational cloning.
Comments: None
Size (bp): 8813
Parent Vector: None
Empty Vector: None
Properties: Gateway, acceptor (destination), lentiviral infection, mammalian expression, recombinational cloning, with tag/fusion/marker
Author Name: Invitrogen
Publications: None
Vector Map:         Vector Sequence:
Sequence:

 



Vector Features:

Type Name Description Start Position End Position
bacterial origin pUC ori pUC origin of replication 7733 8406
death cassette ccdB ccdB death cassette (removed when insert is present) 3767 3976
packaging signal HIV-1 psi HIV-1 psi packaging signal 521 565
poly-A signal SV40 SV40 polyadenylation signal 5639 5770
promoter RSV RSV/5' LTR hybrid promoter 1 410
promoter CMV CMV promoter driving gene of interest 1809 2392
promoter PGK PGK promoter to drive selection marker in mammalian cells 4266 4771
recombination site attR2 attR2 recombinational cloning site 4127 4142
recombination site attR1 attR1 recombinational cloning site 2440 2456
response element HIV-1 RRE HIV-1 Rev response element (RRE) 1075 1308
selectable marker AmpR Ampicillin resistance gene for selection in bacteria 6728 7588
selectable marker BlastR Blasticidin resistance gene in mammalian cells 4849 5249
selectable marker CmR chloramphenicol resistance marker (removed in plasmids with insert) 2673 3329
splice acceptor site 3' splice acceptor two 3' splice acceptors at 1656 and 1684 1656 1656
splice donor site 5' splice donor 5' splice donor 520 520
tag V5 C-terminal V5 epitope tag 4197 4238

 

  • Gene
  • Pathways
  • Protein
  • Clone
  • Organism
  • Genome
  • Reagents

Gene

 

Gene Symbol : TRIM56
Symbol Nomenclature : TRIM56
SYNONYM : RNF109
Designation : E3 ubiquitin-protein ligase TRIM56|RING finger protein 109|tripartite motif-containing 56|tripartite motif-containing protein 56
Full Nomenclature : tripartite motif containing 56
GENEID : 81844
HGNC : 19028
Vega : OTTHUMG00000157032
Target GenBank: BC048194

 

Reference Sequence Alignment

  

HsCD00329663      ATGGTTTCCCACGGGTCCTCGCCCTCCCTCCTGGAGGCCCTGAGCAGCGACTTCCTGGCC
NM_030961.1       ATGGTTTCCCACGGGTCCTCGCCCTCCCTCCTGGAGGCCCTGAGCAGCGACTTCCTGGCC
                  ************************************************************

HsCD00329663      TGTAAAATCTGCCTGGAGCAGCTGCGGGCACCCAAGACACTGCCCTGCCTGCATACCTAC
NM_030961.1       TGTAAAATCTGCCTGGAGCAGCTGCGGGCACCCAAGACACTGCCCTGCCTGCATACCTAC
                  ************************************************************

HsCD00329663      TGCCAAGACTGCCTGGCACAGCTGGCGGATGGCGGCCGCGTCCGCTGCCCCGAGTGCCGC
NM_030961.1       TGCCAAGACTGCCTGGCACAGCTGGCGGATGGCGGCCGCGTCCGCTGCCCCGAGTGCCGC
                  ************************************************************

HsCD00329663      GAGACAGTGCCTGTGCCGCCCGAGGGTGTGGCCTCCTTCAAGACCAACTTCTTCGTCAAT
NM_030961.1       GAGACAGTGCCTGTGCCGCCCGAGGGTGTGGCCTCCTTCAAGACCAACTTCTTCGTCAAT
                  ************************************************************

HsCD00329663      GGGCTGCTGGACCTGGTGAAGGCCCGGGCCTGTGGAGACCTGCGTGCCGGGAAGCCAGCC
NM_030961.1       GGGCTGCTGGACCTGGTGAAGGCCCGGGCCTGTGGAGACCTGCGTGCCGGGAAGCCAGCC
                  ************************************************************

HsCD00329663      TGTGCCCTGTGTCCCCTGGTGGGTGGCACCAGCACCGGGGGGCCGGCCACGGCCCGGTGC
NM_030961.1       TGTGCCCTGTGTCCCCTGGTGGGTGGCACCAGCACCGGGGGGCCGGCCACGGCCCGGTGC
                  ************************************************************

HsCD00329663      CTGGACTGTGCCGATGACTTGTGCCAGGCCTGTGCCGACGGGCACCGCTGCACCCGCCAG
NM_030961.1       CTGGACTGTGCCGATGACTTGTGCCAGGCCTGTGCCGACGGGCACCGCTGCACCCGCCAG
                  ************************************************************

HsCD00329663      ACCCACACCCACCGCGTGGTGGACCTGGTGGGCTACAGGGCCGGGTGGTATGATGAGGAG
NM_030961.1       ACCCACACCCACCGCGTGGTGGACCTGGTGGGCTACAGGGCCGGGTGGTATGATGAGGAG
                  ************************************************************

HsCD00329663      GCCCGGGAGCGCCAAGCGGCCCAGTGTCCCCAGCACCCCGGGGAGGCACTGCGCTTCCTG
NM_030961.1       GCCCGGGAGCGCCAAGCGGCCCAGTGTCCCCAGCACCCCGGGGAGGCACTGCGCTTCCTG
                  ************************************************************

HsCD00329663      TGCCAGCCCTGCTCACAGTTGCTGTGCAGAGAGTGCCGCCTAGACCCCCACCTGGACCAC
NM_030961.1       TGCCAGCCCTGCTCACAGTTGCTGTGCAGAGAGTGCCGCCTAGACCCCCACCTGGACCAC
                  ************************************************************

HsCD00329663      CCCTGCCTGCCTCTGGCTGAAGCTGTGCGTGCCCGGAGGCCGGGCCTGGAGGGACTGCTG
NM_030961.1       CCCTGCCTGCCTCTGGCTGAAGCTGTGCGTGCCCGGAGGCCGGGCCTGGAGGGACTGCTG
                  ************************************************************

HsCD00329663      GCCGGTGTGGACAATAACCTGGTGGAGCTGGAGGCAGCGCGGAGGGTGGAGAAGGAGGCG
NM_030961.1       GCCGGTGTGGACAATAACCTGGTGGAGCTGGAGGCAGCGCGGAGGGTGGAGAAGGAGGCG
                  ************************************************************

HsCD00329663      CTAGCCCGGCTGCGGGAGCAGGCGGCCCGGGTGGGGACTCAGGTGGAGGAGGCGGCTGAG
NM_030961.1       CTAGCCCGGCTGCGGGAGCAGGCGGCCCGGGTGGGGACTCAGGTGGAGGAGGCGGCTGAG
                  ************************************************************

HsCD00329663      GGCGTCCTCCGGGCCCTGCTGGCCCAGAAGCAGGAGGTGCTGGGGCAGCTACGAGCCCAC
NM_030961.1       GGCGTCCTCCGGGCCCTGCTGGCCCAGAAGCAGGAGGTGCTGGGGCAGCTACGAGCCCAC
                  ************************************************************

HsCD00329663      GTGGAGGCTGCCGAAGAAGCTGCTCGGGAGAGGCTGGCGGAGCTTGAGGGCCGGGAGCAG
NM_030961.1       GTGGAGGCTGCCGAAGAAGCTGCTCGGGAGAGGCTGGCGGAGCTTGAGGGCCGGGAGCAG
                  ************************************************************

HsCD00329663      GTGGCCAGGGCGGCAGCCGCCTTCGCCCGCCGGGTACTCAGCCTGGGGCGAGAGGCCGAG
NM_030961.1       GTGGCCAGGGCGGCAGCCGCCTTCGCCCGCCGGGTACTCAGCCTGGGGCGAGAGGCCGAG
                  ************************************************************

HsCD00329663      ATCCTCTCCCTGGAAGGGGCGATCGCACAGCGGCTCAGGCAGCTGCAGGGCTGCCCCTGG
NM_030961.1       ATCCTCTCCCTGGAAGGGGCGATCGCACAGCGGCTCAGGCAGCTGCAGGGCTGCCCCTGG
                  ************************************************************

HsCD00329663      GCACCAGGCCCGGCCCCCTGCCTGCTCCCACAGCTGGAGCTCCATCCTGGGCTCCTGGAC
NM_030961.1       GCACCAGGCCCGGCCCCCTGCCTGCTCCCACAGCTGGAGCTCCATCCTGGGCTCCTGGAC
                  ************************************************************

HsCD00329663      AAGAACTGCCACCTTCTTCGGCTGTCCTTTGAGGAGCAGCAGCCCCAGAAGGATGGTGGG
NM_030961.1       AAGAACTGCCACCTTCTTCGGCTGTCCTTTGAGGAGCAGCAGCCCCAGAAGGATGGTGGG
                  ************************************************************

HsCD00329663      AAAGACGGAGCTGGTACCCAGGGAGGTGAGGAGAGCCAGAGCCGGAGGGAGGATGAGCCG
NM_030961.1       AAAGACGGAGCTGGTACCCAGGGAGGTGAGGAGAGCCAGAGCCGGAGGGAGGATGAGCCG
                  ************************************************************

HsCD00329663      AAGACTGAGAGACAGGGTGGAGTCCAGCCCCAGGCCGGAGATGGAGCCCAGACCCCAAAA
NM_030961.1       AAGACTGAGAGACAGGGTGGAGTCCAGCCCCAGGCCGGAGATGGAGCCCAGACCCCAAAA
                  ************************************************************

HsCD00329663      GAGGAAAAAGCCCAGACAACCCGAGAAGAGGGAGCCCAGACCTTGGAGGAGGACAGGGCC
NM_030961.1       GAGGAAAAAGCCCAGACAACCCGAGAAGAGGGAGCCCAGACCTTGGAGGAGGACAGGGCC
                  ************************************************************

HsCD00329663      CAGACACCCCACGAGGATGGAGGACCCCAGCCCCACAGGGGTGGCAGACCCAACAAGAAG
NM_030961.1       CAGACACCCCACGAGGATGGAGGACCCCAGCCCCACAGGGGTGGCAGACCCAACAAGAAG
                  ************************************************************

HsCD00329663      AAAAAGTTCAAAGGCAGGCTCAAGTCAATTTCCCGGGAGCCCAGCCCAGCCCTGGGGCCG
NM_030961.1       AAAAAGTTCAAAGGCAGGCTCAAGTCAATTTCCCGGGAGCCCAGCCCAGCCCTGGGGCCG
                  ************************************************************

HsCD00329663      AATCTGGACGGCTCTGGCCTCCTCCCCAGACCCATCTTTTACTGCAGTTTCCCCACGCGG
NM_030961.1       AATCTGGACGGCTCTGGCCTCCTCCCCAGACCCATCTTTTACTGCAGTTTCCCCACGCGG
                  ************************************************************

HsCD00329663      ATGCCTGGAGACAAGCGGTCCCCCCGGATCACCGGGCTCTGTCCCTTCGGTCCCCGGGAG
NM_030961.1       ATGCCTGGAGACAAGCGGTCCCCCCGGATCACCGGGCTCTGTCCCTTCGGTCCCCGGGAG
                  ************************************************************

HsCD00329663      ATCCTGGTGGCGGATGAGCAGAACCGGGCACTGAAACGCTTCTCCCTCAACGGCGACTAC
NM_030961.1       ATCCTGGTGGCGGATGAGCAGAACCGGGCACTGAAACGCTTCTCCCTCAACGGCGACTAC
                  ************************************************************

HsCD00329663      AAGGGCACCGTGCCGGTCCCTGAGGGCTGCTCCCCTTGCAGCGTGGCCGCCCTGCAGAGC
NM_030961.1       AAGGGCACCGTGCCGGTCCCTGAGGGCTGCTCCCCTTGCAGCGTGGCCGCCCTGCAGAGC
                  ************************************************************

HsCD00329663      GCGGTGGCCTTCTCCGCTAGCGCACGGCTCTATCTCATCAACCCCAACGGCGAAGTGCAG
NM_030961.1       GCGGTGGCCTTCTCCGCTAGCGCACGGCTCTATCTCATCAACCCCAACGGCGAAGTGCAG
                  ************************************************************

HsCD00329663      TGGCGCAGGGCCCTGAGCCTCTCCCAGGCCAGCCACGCGGTGGCGGCACTGCCTAGCGGG
NM_030961.1       TGGCGCAGGGCCCTGAGCCTCTCCCAGGCCAGCCACGCGGTGGCGGCACTGCCTAGCGGG
                  ************************************************************

HsCD00329663      GACCGCGTGGCTGTCAGCGTGGCGGGCCACGTGGAGGTGTACAATATGGAAGGCAGCCTG
NM_030961.1       GACCGCGTGGCTGTCAGCGTGGCGGGCCACGTGGAGGTGTACAATATGGAAGGCAGCCTG
                  ************************************************************

HsCD00329663      GCCACCCGGTTCATTCCTGGAGGCAAGGCCAGCCGGGGCCTGCGGGCGCTGGTGTTTCTG
NM_030961.1       GCCACCCGGTTCATTCCTGGAGGCAAGGCCAGCCGGGGCCTGCGGGCGCTGGTGTTTCTG
                  ************************************************************

HsCD00329663      ACCACCAGCCCCCAGGGGCATTTCGTGGGGTCGGACTGGCAGCAGAATAGTGTGGTAATC
NM_030961.1       ACCACCAGCCCCCAGGGGCATTTCGTGGGGTCGGACTGGCAGCAGAATAGTGTGGTAATC
                  ************************************************************

HsCD00329663      TGTGATGGGCTGGGCCAGGTGGTTGGGGAGTACAAGGGGCCAGGCCTGCATGGCTGCCAG
NM_030961.1       TGTGATGGGCTGGGCCAGGTGGTTGGGGAGTACAAGGGGCCAGGCCTGCATGGCTGCCAG
                  ************************************************************

HsCD00329663      CCGGGCTCCGTGTCTGTGGATAAGAAGGGCTACATCTTTCTGACCCTTCGAGAAGTCAAC
NM_030961.1       CCGGGCTCCGTGTCTGTGGATAAGAAGGGCTACATCTTTCTGACCCTTCGAGAAGTCAAC
                  ************************************************************

HsCD00329663      AAGGTGGTGATCCTGGACCCGAAGGGGTCCCTCCTTGGAGACTTCCTGACAGCCTACCAC
NM_030961.1       AAGGTGGTGATCCTGGACCCGAAGGGGTCCCTCCTTGGAGACTTCCTGACAGCCTACCAC
                  ************************************************************

HsCD00329663      GGCCTGGAAAAGCCCCGGGTTACCACCATGGTGGATGGCAGGTACCTGGTCGTGTCCCTC
NM_030961.1       GGCCTGGAAAAGCCCCGGGTTACCACCATGGTGGATGGCAGGTACCTGGTCGTGTCCCTC
                  ************************************************************

HsCD00329663      AGTAACGGGACCATCCACATCTTTCGGGTCCGTTCTCCGGACAGTTTG
NM_030961.1       AGTAACGGGACCATCCACATCTTTCGGGTCCGTTCTCCGGACAGTTAA
                  **********************************************:.

Pathways

Reactome : Cytosolic sensors of pathogen-associated DNA
Reactome : Immune System
Reactome : Innate Immune System
Reactome : Regulation of innate immune responses to cytosolic DNA

Protein

SMART domain : BBOX : B-Box-type zinc finger
SMART domain : RING : Ring finger
UniProt : Q9BRZ2
HPRD : 10284

Clone

Cloning Information : 268115
HIP Master Clone ID : 193151
Original Clone ID : FLH268115.01L

Organism


TAX_ID : 9606
Species Specific ID: 81844

Genome

Chromosome : 7
Map Location : 7q22.1
Ensembl : ENSG00000169871

Reagents

Labome : TRIM56-antibody