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PDE7B (Homo sapiens) in pLenti6.2/V5-DEST (V5-tagged lentiviral mammalian expression vector)

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Explanation of Terms


Gene: Gene Symbol:  PDE7B
Gene Name:  phosphodiesterase 7B
Sequence             
Original Clone ID: FLH268118.01L
Keyword: None
Species: Homo sapiens
Type: cDNA
Vector Name: pLenti6.2/V5-DEST               Format:  FUSION
Source: HIP
Description: None
Comments: None
Mutation/
Discrepancy :
No / No
Publications: None
Authors:
HsCD00329665

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AVAILABLE
No restriction
Special MTA: None

 

Insert sequence: 1353nts         Open reading frame : 1 to 1353
Reference Sequence Alignment

ATGTCTTGTTTAATGGTTGAGAGGTGTGGCGAAATCTTGTTTGAGAACCCCGATCAGAAT
GCCAAATGTGTTTGCATGCTGGGAGATATACGACTAAGGGGTCAGACGGGGGTTCGTGCT
GAACGCCGTGGCTCCTACCCATTCATTGACTTCCGCCTACTTAACAGTACAACATACTCA
GGGGAGATTGGCACCAAGAAAAAGGTGAAAAGACTATTAAGCTTTCAAAGATACTTCCAT
GCATCAAGGCTGCTTCGTGGAATTATACCACAAGCCCCTCTGCACCTGCTGGATGAAGAC
TACCTTGGACAAGCAAGGCATATGCTCTCCAAAGTGGGAATGTGGGATTTTGACATTTTC
TTGTTTGATCGCTTGACAAATGGAAACAGCCTGGTAACACTGTTGTGCCACCTCTTCAAT
ACCCATGGACTCATTCACCATTTCAAGTTAGATATGGTGACCTTACACCGATTTTTAGTC
ATGGTTCAAGAAGATTACCACAGCCAAAACCCGTATCACAATGCTGTTCACGCAGCCGAC
GTCACCCAGGCCATGCACTGCTACCTGAAAGAGCCAAAGCTTGCCAGCTTCCTCACGCCT
CTGGACATCATGCTTGGACTGCTGGCTGCAGCAGCACACGATGTGGACCACCCAGGGGTG
AACCAGCCATTTTTGATAAAAACTAACCACCATCTTGCAAACCTATATCAGAATATGTCT
GTGCTGGAGAATCATCACTGGCGATCTACAATTGGCATGCTTCGAGAATCAAGGCTTCTT
GCTCATTTGCCAAAGGAAATGACACAGGATATTGAACAGCAGCTGGGCTCCTTGATCTTG
GCAACAGACATCAACAGGCAGAATGAATTTTTGACCAGATTGAAAGCTCACCTCCACAAT
AAAGACTTAAGACTGGAGGATGCACAGGACAGGCACTTTATGCTTCAGATCGCCTTGAAG
TGTGCTGACATTTGCAATCCTTGTAGAATCTGGGAGATGAGCAAGCAGTGGAGTGAAAGG
GTCTGTGAAGAATTCTACAGGCAAGGTGAACTTGAACAGAAATTTGAACTGGAAATCAGT
CCTCTTTGTAATCAACAGAAAGATTCCATCCCTAGTATACAAATTGGTTTCATGAGCTAC
ATCGTGGAGCCGCTCTTCCGGGAATGGGCCCATTTCACGGGTAACAGCACCCTGTCGGAG
AACATGCTGGGCCACCTCGCACACAACAAGGCCCAGTGGAAGAGCCTGTTGCCCAGGCAG
CACAGAAGCAGGGGCAGCAGTGGCAGCGGGCCTGACCACGACCACGCAGGCCAAGGGACT
GAGAGCGAGGAGCAGGAAGGCGACAGCCCCTTG


Coding Sequence Details
Insert Sequence Verified?: N Verification Method: Insert was fully sequenced in parent vector
Reference Sequence Annotations:
End on reference sequence 1379
Start on reference sequence 27
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
5p GTACAAAAAAGCAGGCTCCACC(atg-goi)

3' Linker Sequence:
5p (goi-stop)GACCCAGCTTTCTTGTAC

 

 Recommended Growth Condition:

Distributed in bacterial strain : DH5-alpha T1 phage resistant
Antibiotic Selection: Host Type:  bacterial    Marker:  ampicillin
Host Type:  mammalian    Marker:  blasticidin
Bacterial Selection Condition:  100 ug/mL ampicillin
Growth Condition:  Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:  Commonly used conditions for ampicillin resistant plasmid clones.
Protein Expression Results: None
Recommended expression in: Not Applicable
       DyNA Vector Map

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Vector Name: pLenti6.2/V5-DEST

pLenti6.2V5-DEST in advanced viewed

Synonyms: pLenti6.2/V5-DEST
Sequencing Primer: Forward:  CMV forward
Reverse:  V5 reverse
Description: Lentiviral mammalian expression vector;CMV promoter and C-terminal V5 tag; ampicillin resistance in bacteria; blasticidin resistance in mammalian cells; Gateway recombinational cloning.
Comments: None
Size (bp): 8813
Parent Vector: None
Empty Vector: None
Properties: Gateway, acceptor (destination), lentiviral infection, mammalian expression, recombinational cloning, with tag/fusion/marker
Author Name: Invitrogen
Publications: None
Vector Map:         Vector Sequence:
Sequence:

 



Vector Features:

Type Name Description Start Position End Position
bacterial origin pUC ori pUC origin of replication 7733 8406
death cassette ccdB ccdB death cassette (removed when insert is present) 3767 3976
packaging signal HIV-1 psi HIV-1 psi packaging signal 521 565
poly-A signal SV40 SV40 polyadenylation signal 5639 5770
promoter RSV RSV/5' LTR hybrid promoter 1 410
promoter CMV CMV promoter driving gene of interest 1809 2392
promoter PGK PGK promoter to drive selection marker in mammalian cells 4266 4771
recombination site attR2 attR2 recombinational cloning site 4127 4142
recombination site attR1 attR1 recombinational cloning site 2440 2456
response element HIV-1 RRE HIV-1 Rev response element (RRE) 1075 1308
selectable marker AmpR Ampicillin resistance gene for selection in bacteria 6728 7588
selectable marker BlastR Blasticidin resistance gene in mammalian cells 4849 5249
selectable marker CmR chloramphenicol resistance marker (removed in plasmids with insert) 2673 3329
splice acceptor site 3' splice acceptor two 3' splice acceptors at 1656 and 1684 1656 1656
splice donor site 5' splice donor 5' splice donor 520 520
tag V5 C-terminal V5 epitope tag 4197 4238

 

  • Gene
  • Pathways
  • Protein
  • Clone
  • Organism
  • Genome
  • Reagents

Gene

 

Gene Symbol : PDE7B
Symbol Nomenclature : PDE7B
SYNONYM : bA472E5.1
Designation : cAMP-specific 3',5'-cyclic phosphodiesterase 7B|high-affinity cAMP-specific 3',5'-cyclic phosphodiesterase|rolipram-insensitive phosphodiesterase type 7
Full Nomenclature : phosphodiesterase 7B
GENEID : 27115
Locus Tag : RP11-472E5.2
HGNC : 8792
MIM : 604645
Vega : OTTHUMG00000015641
Target GenBank: BC075082

 

Reference Sequence Alignment

  

HsCD00329665        ------------------------------------------------------------
XM_005266931.1      ATGCCTGTGCTAGAGCGATATTTCCACCCAGCAGAGCTAGGCAGGAGGTGGACAGGCCCA
NM_018945.3         ------------------------------------------------------------
                                                                                

HsCD00329665        ------------------------------------------------------------
XM_005266931.1      GAAGGTGTGCTGCCCTCCTCCCCGGGAAGCCGGCCGGGGTGCCAGCAGGGGCCGCTGCCC
NM_018945.3         ------------------------------------------------------------
                                                                                

HsCD00329665        ------------------------------------ATGTCTTGTTTAATGGTTGAGAGG
XM_005266931.1      TGGGACTTGCCAGAGATGATCAGGATGGTAAAGCTGGTTTGGAAATCCAAAAGTGAGCTG
NM_018945.3         ------------------------------------ATGTCTTGTTTAATGGTTGAGAGG
                                                        .* *  :.:* .*:.. ****. *

HsCD00329665        TGTGGCGAAATCTTGTTTGAGAACCCCGATCAGAATGCCAAATGTGTTTGCATGCTGGGA
XM_005266931.1      CAGGCGACCAAACAGAGAGGCATTCTGGACAATGAAGATGCTCTCCGCAGCTTTCCAGGA
NM_018945.3         TGTGGCGAAATCTTGTTTGAGAACCCCGATCAGAATGCCAAATGTGTTTGCATGCTGGGA
                     . *  ...*:. :*: :*. *: *  ** .* .*:*. ..:      :**:* * .***

HsCD00329665        GATATACGACTAAGGGGTCAGACGGGGGTTCGTGCTGAACGCCGTGGCTCCTACCCATTC
XM_005266931.1      GATATACGACTAAGGGGTCAGACGGGGGTTCGTGCTGAACGCCGTGGCTCCTACCCATTC
NM_018945.3         GATATACGACTAAGGGGTCAGACGGGGGTTCGTGCTGAACGCCGTGGCTCCTACCCATTC
                    ************************************************************

HsCD00329665        ATTGACTTCCGCCTACTTAACAGTACAACATACTCAGGGGAGATTGGCACCAAGAAAAAG
XM_005266931.1      ATTGACTTCCGCCTACTTAACAGTACAACATACTCAGGGGAGATTGGCACCAAGAAAAAG
NM_018945.3         ATTGACTTCCGCCTACTTAACAGTACAACATACTCAGGGGAGATTGGCACCAAGAAAAAG
                    ************************************************************

HsCD00329665        GTGAAAAGACTATTAAGCTTTCAAAGATACTTCCATGCATCAAGGCTGCTTCGTGGAATT
XM_005266931.1      GTGAAAAGACTATTAAGCTTTCAAAGATACTTCCATGCATCAAGGCTGCTTCGTGGAATT
NM_018945.3         GTGAAAAGACTATTAAGCTTTCAAAGATACTTCCATGCATCAAGGCTGCTTCGTGGAATT
                    ************************************************************

HsCD00329665        ATACCACAAGCCCCTCTGCACCTGCTGGATGAAGACTACCTTGGACAAGCAAGGCATATG
XM_005266931.1      ATACCACAAGCCCCTCTGCACCTGCTGGATGAAGACTACCTTGGACAAGCAAGGCATATG
NM_018945.3         ATACCACAAGCCCCTCTGCACCTGCTGGATGAAGACTACCTTGGACAAGCAAGGCATATG
                    ************************************************************

HsCD00329665        CTCTCCAAAGTGGGAATGTGGGATTTTGACATTTTCTTGTTTGATCGCTTGACAAATGGA
XM_005266931.1      CTCTCCAAAGTGGGAATGTGGGATTTTGACATTTTCTTGTTTGATCGCTTGACAAATGGA
NM_018945.3         CTCTCCAAAGTGGGAATGTGGGATTTTGACATTTTCTTGTTTGATCGCTTGACAAATGGA
                    ************************************************************

HsCD00329665        AACAGCCTGGTAACACTGTTGTGCCACCTCTTCAATACCCATGGACTCATTCACCATTTC
XM_005266931.1      AACAGCCTGGTAACACTGTTGTGCCACCTCTTCAATACCCATGGACTCATTCACCATTTC
NM_018945.3         AACAGCCTGGTAACACTGTTGTGCCACCTCTTCAATACCCATGGACTCATTCACCATTTC
                    ************************************************************

HsCD00329665        AAGTTAGATATGGTGACCTTACACCGATTTTTAGTCATGGTTCAAGAAGATTACCACAGC
XM_005266931.1      AAGTTAGATATGGTGACCTTACACCGATTTTTAGTCATGGTTCAAGAAGATTACCACAGC
NM_018945.3         AAGTTAGATATGGTGACCTTACACCGATTTTTAGTCATGGTTCAAGAAGATTACCACAGC
                    ************************************************************

HsCD00329665        CAAAACCCGTATCACAATGCTGTTCACGCAGCCGACGTCACCCAGGCCATGCACTGCTAC
XM_005266931.1      CAAAACCCGTATCACAATGCTGTTCACGCAGCCGACGTCACCCAGGCCATGCACTGCTAC
NM_018945.3         CAAAACCCGTATCACAATGCTGTTCACGCAGCCGACGTCACCCAGGCCATGCACTGCTAC
                    ************************************************************

HsCD00329665        CTGAAAGAGCCAAAGCTTGCCAGCTTCCTCACGCCTCTGGACATCATGCTTGGACTGCTG
XM_005266931.1      CTGAAAGAGCCAAAGCTTGCCAGCTTCCTCACGCCTCTGGACATCATGCTTGGACTGCTG
NM_018945.3         CTGAAAGAGCCAAAGCTTGCCAGCTTCCTCACGCCTCTGGACATCATGCTTGGACTGCTG
                    ************************************************************

HsCD00329665        GCTGCAGCAGCACACGATGTGGACCACCCAGGGGTGAACCAGCCATTTTTGATAAAAACT
XM_005266931.1      GCTGCAGCAGCACACGATGTGGACCACCCAGGGGTGAACCAGCCATTTTTGATAAAAACT
NM_018945.3         GCTGCAGCAGCACACGATGTGGACCACCCAGGGGTGAACCAGCCATTTTTGATAAAAACT
                    ************************************************************

HsCD00329665        AACCACCATCTTGCAAACCTATATCAGAATATGTCTGTGCTGGAGAATCATCACTGGCGA
XM_005266931.1      AACCACCATCTTGCAAACCTATATCAGAATATGTCTGTGCTGGAGAATCATCACTGGCGA
NM_018945.3         AACCACCATCTTGCAAACCTATATCAGAATATGTCTGTGCTGGAGAATCATCACTGGCGA
                    ************************************************************

HsCD00329665        TCTACAATTGGCATGCTTCGAGAATCAAGGCTTCTTGCTCATTTGCCAAAGGAAATGACA
XM_005266931.1      TCTACAATTGGCATGCTTCGAGAATCAAGGCTTCTTGCTCATTTGCCAAAGGAAATGACA
NM_018945.3         TCTACAATTGGCATGCTTCGAGAATCAAGGCTTCTTGCTCATTTGCCAAAGGAAATGACA
                    ************************************************************

HsCD00329665        CAGGATATTGAACAGCAGCTGGGCTCCTTGATCTTGGCAACAGACATCAACAGGCAGAAT
XM_005266931.1      CAGGATATTGAACAGCAGCTGGGCTCCTTGATCTTGGCAACAGACATCAACAGGCAGAAT
NM_018945.3         CAGGATATTGAACAGCAGCTGGGCTCCTTGATCTTGGCAACAGACATCAACAGGCAGAAT
                    ************************************************************

HsCD00329665        GAATTTTTGACCAGATTGAAAGCTCACCTCCACAATAAAGACTTAAGACTGGAGGATGCA
XM_005266931.1      GAATTTTTGACCAGATTGAAAGCTCACCTCCACAATAAAGACTTAAGACTGGAGGATGCA
NM_018945.3         GAATTTTTGACCAGATTGAAAGCTCACCTCCACAATAAAGACTTAAGACTGGAGGATGCA
                    ************************************************************

HsCD00329665        CAGGACAGGCACTTTATGCTTCAGATCGCCTTGAAGTGTGCTGACATTTGCAATCCTTGT
XM_005266931.1      CAGGACAGGCACTTTATGCTTCAGATCGCCTTGAAGTGTGCTGACATTTGCAATCCTTGT
NM_018945.3         CAGGACAGGCACTTTATGCTTCAGATCGCCTTGAAGTGTGCTGACATTTGCAATCCTTGT
                    ************************************************************

HsCD00329665        AGAATCTGGGAGATGAGCAAGCAGTGGAGTGAAAGGGTCTGTGAAGAATTCTACAGGCAA
XM_005266931.1      AGAATCTGGGAGATGAGCAAGCAGTGGAGTGAAAGGGTCTGTGAAGAATTCTACAGGCAA
NM_018945.3         AGAATCTGGGAGATGAGCAAGCAGTGGAGTGAAAGGGTCTGTGAAGAATTCTACAGGCAA
                    ************************************************************

HsCD00329665        GGTGAACTTGAACAGAAATTTGAACTGGAAATCAGTCCTCTTTGTAATCAACAGAAAGAT
XM_005266931.1      GGTGAACTTGAACAGAAATTTGAACTGGAAATCAGTCCTCTTTGTAATCAACAGAAAGAT
NM_018945.3         GGTGAACTTGAACAGAAATTTGAACTGGAAATCAGTCCTCTTTGTAATCAACAGAAAGAT
                    ************************************************************

HsCD00329665        TCCATCCCTAGTATACAAATTGGTTTCATGAGCTACATCGTGGAGCCGCTCTTCCGGGAA
XM_005266931.1      TCCATCCCTAGTATACAAATTGGTTTCATGAGCTACATCGTGGAGCCGCTCTTCCGGGAA
NM_018945.3         TCCATCCCTAGTATACAAATTGGTTTCATGAGCTACATCGTGGAGCCGCTCTTCCGGGAA
                    ************************************************************

HsCD00329665        TGGGCCCATTTCACGGGTAACAGCACCCTGTCGGAGAACATGCTGGGCCACCTCGCACAC
XM_005266931.1      TGGGCCCATTTCACGGGTAACAGCACCCTGTCGGAGAACATGCTGGGCCACCTCGCACAC
NM_018945.3         TGGGCCCATTTCACGGGTAACAGCACCCTGTCGGAGAACATGCTGGGCCACCTCGCACAC
                    ************************************************************

HsCD00329665        AACAAGGCCCAGTGGAAGAGCCTGTTGCCCAGGCAGCACAGAAGCAGGGGCAGCAGTGGC
XM_005266931.1      AACAAGGCCCAGTGGAAGAGCCTGTTGCCCAGGCAGCACAGAAGCAGGGGCAGCAGTGGC
NM_018945.3         AACAAGGCCCAGTGGAAGAGCCTGTTGCCCAGGCAGCACAGAAGCAGGGGCAGCAGTGGC
                    ************************************************************

HsCD00329665        AGCGGGCCTGACCACGACCACGCAGGCCAAGGGACTGAGAGCGAGGAGCAGGAAGGCGAC
XM_005266931.1      AGCGGGCCTGACCACGACCACGCAGGCCAAGGGACTGAGAGCGAGGAGCAGGAAGGCGAC
NM_018945.3         AGCGGGCCTGACCACGACCACGCAGGCCAAGGGACTGAGAGCGAGGAGCAGGAAGGCGAC
                    ************************************************************

HsCD00329665        AGCCCCTTG
XM_005266931.1      AGCCCCTAG
NM_018945.3         AGCCCCTAG
                    *******:*

Pathways

Reactome : G alpha (s) signalling events
Reactome : GPCR downstream signaling
Reactome : Signal Transduction
Reactome : Signaling by GPCR
WikiPathway : G Protein Signaling Pathways

Clone

Cloning Information : 268118
HIP Master Clone ID : 193154
Original Clone ID : FLH268118.01L

Organism


TAX_ID : 9606
Species Specific ID: 27115

Genome

Chromosome : 6
Map Location : 6q23-q24
Ensembl : ENSG00000171408

Reagents

Labome : PDE7B-antibody