DNASU Plasmid Repository • 480.965.5697 | Email

TRIM49 (Homo sapiens) in pLenti6.2/V5-DEST (V5-tagged lentiviral mammalian expression vector)

TOP

Explanation of Terms


Gene: Gene Symbol:  TRIM49
Gene Name:  tripartite motif containing 49
Sequence             
Original Clone ID: FLH268119.01L
Keyword: None
Species: Homo sapiens
Type: cDNA
Vector Name: pLenti6.2/V5-DEST               Format:  FUSION
Source: HIP
Description: None
Comments: None
Mutation/
Discrepancy :
No / No
Publications: None
Authors:
HsCD00329666

Price:  Login for Pricing
AVAILABLE
No restriction
Special MTA: None

 

Insert sequence: 1359nts         Open reading frame : 1 to 1359
Reference Sequence Alignment

ATGAATTCTGGAATCTTACAGGTCTTTCAGGGGGAACTCATCTGCCCCCTGTGCATGAAC
TACTTCATAGACCCGGTCACCATAGACTGTGGGCACAGCTTTTGCAGGCCTTGTTTCTAC
CTCAACTGGCAAGACATCCCATTTCTTGTCCAGTGCTCTGAATGCACAAAGTCAACAGAG
CAGATAAACCTCAAAACCAACATTCATTTGAAGAAGATGGCTTCTCTTGCCAGAAAAGTC
AGTCTCTGGCTATTCCTGAGCTCTGAGGAGCAAATGTGTGGCACTCACAGGGAGACAAAG
AAGATATTCTGTGAAGTGGACAGGAGCCTGCTCTGTTTGCTGTGCTCCAGCTCTCAGGAG
CACCGGTATCACAGACACCGTCCCATTGAGTGGGCTGCTGAGGAACACCGGGAGAAGCTT
TTACAGAAAATGCAGTCTTTGTGGGAAAAAGCTTGTGAAAATCACAGAAACCTGAATGTG
GAAACCACCAGAACCAGATGCTGGAAGGATTATGTGAATTTAAGGCTAGAAGCAATTAGA
GCTGAGTATCAGAAGATGCCTGCATTTCATCATGAAGAAGAAAAACATAATTTGGAGATG
CTGAAAAAGAAGGGGAAAGAAATTTTTCATCGACTTCATTTAAGTAAAGCCAAAATGGCT
CATAGGATGGAGATTTTAAGAGGAATGTATGAGGAGCTGAACGAAATGTGCCATAAACCA
GATGTGGAGCTACTTCAGGCTTTTGGAGACATATTACACAGGAGTGAGTCCGTGCTGCTG
CACATGCCCCAGCCTCTGAATCCAGAGCTCAGTGCAGGGCCCATCACTGGACTGAGGGAC
AGGCTCAACCAATTCCGAGTGCATATTACTCTGCATCATGAAGAAGCCAACAGTGATATC
TTTCTGTATGAAATTTTGAGAAGCATGTGTATTGGATGTGACCATCAAGATGTACCCTAT
TTCACTGCAACACCTAGAAGTTTTCTTGCATGGGGTGTTCAGACTTTCACCTCGGGCAAA
TATTACTGGGAGGTCCATGTAGGGGACTCCTGGAATTGGGCTTTTGGTGTCTGTAATATG
TATCGGAAAGAGAAGAATCAGAATGAGAAGATAGATGGAAAGGCGGGACTCTTTCTTCTT
GGGTGTGTTAAGAATGACATTCAATGCAGTCTCTTTACCACCTCCCCACTTATGCTGCAA
TATATCCCAAAACCTACCAGCCGAGTAGGATTATTCCTGGATTGTGAGGCTAAGACTGTG
AGCTTTGTTGATGTTAATCAAAGCTCCCTAATATACACCATCCCTAATTGCTCTTTCTCA
CCTCCTCTCAGGCCTATCTTTTGCTGTATTCACTTCTTG


Coding Sequence Details
Insert Sequence Verified?: N Verification Method: Insert was fully sequenced in parent vector
Reference Sequence Annotations:
End on reference sequence 1387
Start on reference sequence 29
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
5p GTACAAAAAAGCAGGCTCCACC(atg-goi)

3' Linker Sequence:
5p (goi-stop)GACCCAGCTTTCTTGTAC

 

 Recommended Growth Condition:

Distributed in bacterial strain : DH5-alpha T1 phage resistant
Antibiotic Selection: Host Type:  bacterial    Marker:  ampicillin
Host Type:  mammalian    Marker:  blasticidin
Bacterial Selection Condition:  100 ug/mL ampicillin
Growth Condition:  Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:  Commonly used conditions for ampicillin resistant plasmid clones.
Protein Expression Results: None
Recommended expression in: Not Applicable
       DyNA Vector Map

         Powered by LabGenius

Vector Name: pLenti6.2/V5-DEST

pLenti6.2V5-DEST in advanced viewed

Synonyms: pLenti6.2/V5-DEST
Sequencing Primer: Forward:  CMV forward
Reverse:  V5 reverse
Description: Lentiviral mammalian expression vector;CMV promoter and C-terminal V5 tag; ampicillin resistance in bacteria; blasticidin resistance in mammalian cells; Gateway recombinational cloning.
Comments: None
Size (bp): 8813
Parent Vector: None
Empty Vector: None
Properties: Gateway, acceptor (destination), lentiviral infection, mammalian expression, recombinational cloning, with tag/fusion/marker
Author Name: Invitrogen
Publications: None
Vector Map:         Vector Sequence:
Sequence:

 



Vector Features:

Type Name Description Start Position End Position
bacterial origin pUC ori pUC origin of replication 7733 8406
death cassette ccdB ccdB death cassette (removed when insert is present) 3767 3976
packaging signal HIV-1 psi HIV-1 psi packaging signal 521 565
poly-A signal SV40 SV40 polyadenylation signal 5639 5770
promoter RSV RSV/5' LTR hybrid promoter 1 410
promoter CMV CMV promoter driving gene of interest 1809 2392
promoter PGK PGK promoter to drive selection marker in mammalian cells 4266 4771
recombination site attR2 attR2 recombinational cloning site 4127 4142
recombination site attR1 attR1 recombinational cloning site 2440 2456
response element HIV-1 RRE HIV-1 Rev response element (RRE) 1075 1308
selectable marker AmpR Ampicillin resistance gene for selection in bacteria 6728 7588
selectable marker BlastR Blasticidin resistance gene in mammalian cells 4849 5249
selectable marker CmR chloramphenicol resistance marker (removed in plasmids with insert) 2673 3329
splice acceptor site 3' splice acceptor two 3' splice acceptors at 1656 and 1684 1656 1656
splice donor site 5' splice donor 5' splice donor 520 520
tag V5 C-terminal V5 epitope tag 4197 4238

 

  • Gene
  • Protein
  • Clone
  • Organism
  • Genome
  • Reagents

Gene

 

Gene Symbol : TRIM49
Symbol Nomenclature : TRIM49
SYNONYM : RNF18; TRIM49A; TRIM49L2
Designation : ring finger protein 18|testis-specific RING Finger protein|testis-specific ring-finger protein|tripartite motif-containing 49|tripartite motif-containing protein 49
Full Nomenclature : tripartite motif containing 49
GENEID : 57093
HGNC : 13431
MIM : 606124
Vega : OTTHUMG00000167634
Target GenBank: BC075020

 

Reference Sequence Alignment

  

HsCD00329666        ATGAATTCTGGAATCTTACAGGTCTTTCAGGGGGAACTCATCTGCCCCCTGTGCATGAAC
NM_020358.2         ATGAATTCTGGAATCTTACAGGTCTTTCAGGGGGAACTCATCTGCCCCCTGTGCATGAAC
NM_001195234.1      ATGAATTCTGGAATCTTACAGGTCTTTCAGGGGGAACTCATCTGCCCCCTGTGCATGAAC
NM_001206626.1      ATGAATTCTGGAATCTTACAGGTCTTTCAGAGGGAACTCATCTGCCCCATCTGCATGAAC
                    ******************************.*****************.* *********

HsCD00329666        TACTTCATAGACCCGGTCACCATAGACTGTGGGCACAGCTTTTGCAGGCCTTGTTTCTAC
NM_020358.2         TACTTCATAGACCCGGTCACCATAGACTGTGGGCACAGCTTTTGCAGGCCTTGTTTCTAC
NM_001195234.1      TACTTCATAGACCCGGTCACCATAGACTGTGGGCACAGCTTTTGCAGGCCTTGTTTCTAC
NM_001206626.1      TACTTCATAGACCCGGTCACCATAGACTGTGGGCACAGCTTTTGCAGGCCTTGTTTCTAC
                    ************************************************************

HsCD00329666        CTCAACTGGCAAGACATCCCATTTCTTGTCCAGTGCTCTGAATGCACAAAGTCAACAGAG
NM_020358.2         CTCAACTGGCAAGACATCCCATTTCTTGTCCAGTGCTCTGAATGCACAAAGTCAACCGAG
NM_001195234.1      CTCAACTGGCAAGACATCCCATTTCTTGTCCAGTGCTCTGAATGCACAAAGTCAACAGAG
NM_001206626.1      CTCAACTGGAAAGACAGCCCATTTCTTGTCCAGTGCTCTGAATGCACAAAGTCAACAGGG
                    *********.****** ***************************************.*.*

HsCD00329666        CAGATAAACCTCAAAACCAACATTCATTTGAAGAAGATGGCTTCTCTTGCCAGAAAAGTC
NM_020358.2         CAGATAAACCTCAAAACCAACATTCATTTGAAGAAGATGGCTTCTCTTGCCAGAAAAGTC
NM_001195234.1      CAGATAAACCTCAAAACCAACATTCATTTGAAGAAGATGGCTTCTCTTGCCAGAAAAGTC
NM_001206626.1      CAGATAAACCTCAAAACCAACATTCATTTCAAGAAGATGGCTTCTCTTGCTAGAAAAGTC
                    ***************************** ******************** *********

HsCD00329666        AGTCTCTGGCTATTCCTGAGCTCTGAGGAGCAAATGTGTGGCACTCACAGGGAGACAAAG
NM_020358.2         AGTCTCTGGCTATTCCTGAGCTCTGAGGAGCAAATGTGTGGCACTCACAGGGAGACAAAG
NM_001195234.1      AGTCTCTGGCTATTCCTGAGCTCTGAGGAGCAAATGTGTGGCACTCACAGGGAGACAAAG
NM_001206626.1      AGTCTCTGGCTATTCCTGAGCTCTGAGGAGCAAATGTGTGGCACTCACAGGGAGACAAAG
                    ************************************************************

HsCD00329666        AAGATATTCTGTGAAGTGGACAGGAGCCTGCTCTGTTTGCTGTGCTCCAGCTCTCAGGAG
NM_020358.2         AAGATATTCTGTGAAGTGGACAGGAGCCTGCTCTGTTTGCTGTGCTCCAGCTCTCAGGAG
NM_001195234.1      AAGATATTCTGTGAAGTGGACAGGAGCCTGCTCTGTTTGCTGTGCTCCAGCTCTCAGGAG
NM_001206626.1      AAGATGTTCTGTGAAGTGGACAGGAGCCTGCTCTGTTTGCTGTGCTCCAGCTCTCAGGAG
                    *****.******************************************************

HsCD00329666        CACCGGTATCACAGACACCGTCCCATTGAGTGGGCTGCTGAGGAACACCGGGAGAAGCTT
NM_020358.2         CACCGGTATCACAGACACCGTCCCATTGAGTGGGCTGCTGAGGAACACCGGGAGAAGCTT
NM_001195234.1      CACCGGTATCACAGACACCGTCCCATTGAGTGGGCTGCTGAGGAACACCGGGAGAAGCTT
NM_001206626.1      CACCGGGATCACAGACACTGTCCCATTGAGTCGGCTGCTGAGGAACACCAGGAGAAGCTT
                    ****** *********** ************ *****************.**********

HsCD00329666        TTACAGAAAATGCAGTCTTTGTGGGAAAAAGCTTGTGAAAATCACAGAAACCTGAATGTG
NM_020358.2         TTACAGAAAATGCAGTCTTTGTGGGAAAAAGCTTGTGAAAATCACAGAAACCTGAATGTG
NM_001195234.1      TTACAGAAAATGCAGTCTTTGTGGGAAAAAGCTTGTGAAAATCACAGAAACCTGAATGTG
NM_001206626.1      TTACAGAAAATGCAGTCTTTGTGGGAAAAAGCTTGTGAAAATCACAGAAACCTGAATGTG
                    ************************************************************

HsCD00329666        GAAACCACCAGAACCAGATGCTGGAAGGATTATGTGAATTTAAGGCTAGAAGCAATTAGA
NM_020358.2         GAAACCACCAGAACCAGATGCTGGAAGGATTATGTGAATTTAAGGCTAGAAGCAATTAGA
NM_001195234.1      GAAACCACCAGAACCAGATGCTGGAAGGATTATGTGAATTTAAGGCTAGAAGCAATTAGA
NM_001206626.1      GAAACCACCAGAACCAGATGCTGGAAGGATTATGTGAATTTAAGGCTAGAAGCAATTAGA
                    ************************************************************

HsCD00329666        GCTGAGTATCAGAAGATGCCTGCATTTCATCATGAAGAAGAAAAACATAATTTGGAGATG
NM_020358.2         GCTGAGTATCAGAAGATGCCTGCATTTCATCATGAAGAAGAAAAACATAATTTGGAGATG
NM_001195234.1      GCTGAGTATCAGAAGATGCCTGCATTTCATCATGAAGAAGAAAAACATAATTTGGAGATG
NM_001206626.1      GCTGAGTATCAGAAGATGCCTGCATTTCACCATGAAGAAGAAAAACATAATTTGGAGATG
                    ***************************** ******************************

HsCD00329666        CTGAAAAAGAAGGGGAAAGAAATTTTTCATCGACTTCATTTAAGTAAAGCCAAAATGGCT
NM_020358.2         CTGAAAAAGAAGGGGAAAGAAATTTTTCATCGACTTCATTTAAGTAAAGCCAAAATGGCT
NM_001195234.1      CTGAAAAAGAAGGGGAAAGAAATTTTTCATCGACTTCATTTAAGTAAAGCCAAAATGGCT
NM_001206626.1      CTGAAAAAGAAGGGGAAAGATATTTTTCATCGACTTCATTTAAGTAAAGCCAAAATGGCT
                    ********************:***************************************

HsCD00329666        CATAGGATGGAGATTTTAAGAGGAATGTATGAGGAGCTGAACGAAATGTGCCATAAACCA
NM_020358.2         CATAGGATGGAGATTTTAAGAGGAATGTATGAGGAGCTGAACGAAATGTGCCATAAACCA
NM_001195234.1      CATAGGATGGAGATTTTAAGAGGAATGTATGAGGAGCTGAACGAAATGTGCCATAAACCA
NM_001206626.1      CATAGGAGGGAGATTTTAAGAGGAATGTATGAGGAGCTGAACGAAATGTGCCATAAACCA
                    ******* ****************************************************

HsCD00329666        GATGTGGAGCTACTTCAGGCTTTTGGAGACATATTACACAGGAGTGAGTCCGTGCTGCTG
NM_020358.2         GATGTGGAGCTACTTCAGGCTTTTGGAGACATATTACACAGGAGTGAGTCCGTGCTGCTG
NM_001195234.1      GATGTGGAGCTACTTCAGGCTTTTGGAGACATATTACACAGGAGTGAGTCCGTGCTGCTG
NM_001206626.1      GATGTGGAGCTACTTCAGGCTTTTGGAGACATATTACACAGGAGTGAGTCCGTGCTGCTG
                    ************************************************************

HsCD00329666        CACATGCCCCAGCCTCTGAATCCAGAGCTCAGTGCAGGGCCCATCACTGGACTGAGGGAC
NM_020358.2         CACATGCCCCAGCCTCTGAATCCAGAGCTCAGTGCAGGGCCCATCACTGGACTGAGGGAC
NM_001195234.1      CACATGCCCCAGCCTCTGAATCCAGAGCTCAGTGCAGGGCCCATCACTGGACTGAGGGAC
NM_001206626.1      CACATGCCCCAGCCTCTGAATCCAGAGCTCAGTGCAGGGCCCATCACTGGACTGAGGGAC
                    ************************************************************

HsCD00329666        AGGCTCAACCAATTCCGAGTGCATATTACTCTGCATCATGAAGAAGCCAACAGTGATATC
NM_020358.2         AGGCTCAACCAATTCCGAGTGCATATTACTCTGCATCATGAAGAAGCCAACAATGATATC
NM_001195234.1      AGGCTCAACCAATTCCGAGTGCATATTACTCTGCATCATGAAGAAGCCAACAGTGATATC
NM_001206626.1      AGGCTCAACCAATTCCGAGTGCATATTACTCTGCATCATGAAGAAGCCAACAGTGATATC
                    ****************************************************.*******

HsCD00329666        TTTCTGTATGAAATTTTGAGAAGCATGTGTATTGGATGTGACCATCAAGATGTACCCTAT
NM_020358.2         TTTCTGTATGAAATTTTGAGAAGCATGTGTATTGGATGTGACCATCAAGATGTACCCTAT
NM_001195234.1      TTTCTGTATGAAATTTTGAGAAGCATGTGTATTGGATGTGACCATCAAGATGTACCCTAT
NM_001206626.1      TTTCTATGTGAAATTTTGAGAAGCATGTGTATTGGATGTGACCATCAAGATGTACCCTAT
                    *****.*.****************************************************

HsCD00329666        TTCACTGCAACACCTAGAAGTTTTCTTGCATGGGGTGTTCAGACTTTCACCTCGGGCAAA
NM_020358.2         TTCACTGCAACACCTAGAAGTTTTCTTGCATGGGGTGTTCAGACTTTCACCTCGGGCAAA
NM_001195234.1      TTCACTGCAACACCTAGAAGTTTTCTTGCATGGGGTGTTCAGACTTTCACCTCGGGCAAA
NM_001206626.1      TTCACTGCAACACCTAGAAGTTTTCTTGCATGGGGTGCTCAGACTTTCACCTCGGGCAAA
                    ************************************* **********************

HsCD00329666        TATTACTGGGAGGTCCATGTAGGGGACTCCTGGAATTGGGCTTTTGGTGTCTGTAATATG
NM_020358.2         TATTACTGGGAGGTCCATGTAGGGGACTCCTGGAATTGGGCTTTTGGTGTCTGTAATATG
NM_001195234.1      TATTACTGGGAGGTCCATGTAGGGGACTCCTGGAATTGGGCTTTTGGTGTCTGTAATATG
NM_001206626.1      TATTACTGGGAGGTCCATGTAGGGGACTCCTGGAATTGGGCTTTTGGTGTCTGTAATATG
                    ************************************************************

HsCD00329666        TATCGGAAAGAGAAGAATCAGAATGAGAAGATAGATGGAAAGGCGGGACTCTTTCTTCTT
NM_020358.2         TATCGGAAAGAGAAGAATCAGAATGAGAAGATAGATGGAAAGGCGGGACTCTTTCTTCTT
NM_001195234.1      TATCGGAAGGAGAAGAATCAGAATGAGAAGATAGATGGAAAGGAGGGACTCTTTCTTCTT
NM_001206626.1      TATTGGAAAGAGAAGAATCAGAATGAGAAGATAGATGGAGAGGATGGACTCTTTCTTCTT
                    *** ****.******************************.***. ***************

HsCD00329666        GGGTGTGTTAAGAATGACATTCAATGCAGTCTCTTTACCACCTCCCCACTTATGCTGCAA
NM_020358.2         GGGTGTGTTAAGAATGACATTCAATGCAGTCTCTTTACCACCTCCCCACTTATGCTGCAA
NM_001195234.1      GGGTGTATTAAGAATGACATTCAATGCAGTCTCTTTACCACCTCCCCACTTATGCTGCAA
NM_001206626.1      GGGTGTGTTAAGAATGACATTCAACGCAGTCTCTTTACCACCTCCCCACTTCTGCTGCAA
                    ******.***************** **************************.********

HsCD00329666        TATATCCCAAAACCTACCAGCCGAGTAGGATTATTCCTGGATTGTGAGGCTAAGACTGTG
NM_020358.2         TATATCCCAAAACCTACCAGCCGAGTAGGATTATTCCTGGATTGTGAGGCTAAGACTGTG
NM_001195234.1      TATATCCCAAAACCTACCAGCCGAGTAGGATTATTCCTGGATTGTGAGGCTAAGACTGTG
NM_001206626.1      TATATCCCAAGACCTACCAGCCGAGTAGGATTATTCCTGGATTGTGAGGCTAAGACTGTG
                    **********.*************************************************

HsCD00329666        AGCTTTGTTGATGTTAATCAAAGCTCCCTAATATACACCATCCCTAATTGCTCTTTCTCA
NM_020358.2         AGCTTTGTTGATGTTAATCAAAGCTCCCTAATATACACCATCCCTAATTGCTCTTTCTCA
NM_001195234.1      AGCTTTGTTGATGTTAATCAAAGCTCCCTAATATACACCATCCCTAATTGCTCTTTCTCA
NM_001206626.1      AGCTTTGTTGATGTTAATCAAAGCTCCCTAATATACACCATCCCTAATTGCTCTTTCTCA
                    ************************************************************

HsCD00329666        CCTCCTCTCAGGCCTATCTTTTGCTGTATTCACTTCTTG
NM_020358.2         CCTCCTCTCAGGCCTATCTTTTGCTGTATTCACTTCTGA
NM_001195234.1      CCTCCTCTCAGGCCTATCTTTTGCTGTATTCACTTCTGA
NM_001206626.1      CCTCCTCTCAGGCCTATCTTTTGCTGTATTCACTTCTGA
                    ************************************* .

Clone

Cloning Information : 268119
HIP Master Clone ID : 193155
Original Clone ID : FLH268119.01L

Organism


TAX_ID : 9606
Species Specific ID: 57093

Genome

Chromosome : 11
Map Location : 11p11.12-q12
Ensembl : ENSG00000168930

Reagents

Labome : RNF18-antibody