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C1orf181 (H. sapiens ) in pLenti6.2/V5-DEST

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Explanation of Terms


Gene: Gene Symbol:  ZNHIT6
Gene Name:  zinc finger, HIT-type containing 6
Sequence             
Original Clone ID: FLH268136.01L
Keyword: None
Species: Homo sapiens
Type: cDNA
Vector Name: pLenti6.2/V5-DEST               Format:  FUSION
Source: HIP
Description: None
Comments: None
Mutation/
Discrepancy :
No / No
Publications: None
Authors:
HsCD00329679

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AVAILABLE
No restriction
Special MTA: None

 

Insert sequence: 1413nts         Open reading frame : 1 to 1413
Reference Sequence Alignment

ATGGAGTTTGCTGCTGAAAATGAAGGGAAGTCTGGGGGAGGTCTCCACAGCGTAGCTGAG
GGGGTGCGGCTAAGTCCAGAGCCTGGCAGGGAGGGAGTAAGGGACTTAGCAGGGGCGGAG
GAGTTCGGCGGCGGAGAGGAGGGGACAGGGCTGACAGGGATAAAGGAGATAGGGGATGGA
GAGGAAGGAAGTGGACAAAGGCCAGAGGAAATACCGATGGACCTAACGGTAGTGAAGCAG
GAAATTATAGACTGGCCAGGTACAGAAGGCAGGTTGGCTGGCCAGTGGGTAGAACAGGAG
GTGGAGGATAGGCCTGAGGTGAAGGATGAGAACGCAGGCGTATTGGAGGTGAAGCAGGAG
ACGGATAGTAGTTTAGTGGTAAAAGAAGCGAAGGTGGGTGAACCAGAGGTAAAGGAAGAG
AAGGTAAAGGAAGAGGTAATGGACTGGTCAGAAGTGAAGGAAGAGAAGGATAACTTGGAG
ATAAAACAGGAGGAGAAGTTTGTTGGTCAATGCATAAAAGAGGAATTGATGCATGGAGAG
TGTGTAAAAGAAGAGAAGGATTTCCTGAAGAAAGAAATCGTGGATGATACAAAGGTGAAA
GAAGAGCCTCCGATAAATCACCCGGTGGGCTGCAAGCGGAAACTGGCCATGTCAAGGTGT
GAGACTTGTGGTACAGAAGAAGCAAAGTACAGATGTCCACGTTGTATGCGATATTCCTGC
AGTTTGCCCTGTGTAAAGAAACACAAAGCAGAACTGACATGTAATGGAGTTCGAGATAAA
ACTGCATACATTTCAATACAACAGTTTACTGAAATGAATCTCCTAAGTGATTATCGATTT
TTGGAAGATGTGGCAAGAACAGCGGACCATATTTCTAGAGATGCTTTTTTGAAGAGACCA
ATAAGCAATAAATATATGTACTTTATGAAAAATCGTGCCCGGAGGCAAGGTATTAACTTA
AAACTTCTACCCAATGGATTCACCAAGAGGAAGGAGAATTCAACCTTTTTTGATAAGAAA
AAACAACAGTTTTGTTGGCATGTGAAGCTCCAGTTTCCTCAAAGTCAAGCTGAGTACATA
GAAAAAAGAGTACCAGATGATAAAACTATTAATGAAATCCTAAAACCTTACATTGATCCT
GAAAAGTCTGATCCTGTAATTCGTCAAAGGTTGAAAGCCTACATTCGCTCTCAGACTGGG
GTTCAGATTTTAATGAAGATTGAATATATGCAGCAAAATTTAGTAAGATATTATGAACTA
GATCCTTATAAAAGTCTCCTAGACAATTTGAGGAACAAAGTGATCATTGAGTATCCAACA
TTACATGTGGTATTGAAAGGATCCAATAATGACATGAAAGTTCTTCACCAAGTGAAGAGT
GAATCTACCAAGAACGTTGGCAATGAAAATTTG


Coding Sequence Details
Insert Sequence Verified?: N Verification Method: Insert was fully sequenced in parent vector
Reference Sequence Annotations:
End on reference sequence 1480
Start on reference sequence 68
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
5p GTACAAAAAAGCAGGCTCCACC(atg-goi)

3' Linker Sequence:
5p (goi-stop)GACCCAGCTTTCTTGTAC

 

 Recommended Growth Condition:

Distributed in bacterial strain : DH5-alpha T1 phage resistant
Antibiotic Selection: Host Type:  bacterial    Marker:  ampicillin
Host Type:  mammalian    Marker:  blasticidin
Bacterial Selection Condition:  100 ug/mL ampicillin
Growth Condition:  Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:  Commonly used conditions for ampicillin resistant plasmid clones.
Protein Expression Results: None
Recommended expression in: Not Applicable
       DyNA Vector Map

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Vector Name: pLenti6.2/V5-DEST

pLenti6.2V5-DEST in advanced viewed

Synonyms: pLenti6.2/V5-DEST
Sequencing Primer: Forward:  CMV forward
Reverse:  V5 reverse
Description: Lentiviral mammalian expression vector;CMV promoter and C-terminal V5 tag; ampicillin resistance in bacteria; blasticidin resistance in mammalian cells; Gateway recombinational cloning.
Comments: None
Size (bp): 8813
Parent Vector: None
Empty Vector: None
Properties: Gateway, acceptor (destination), lentiviral infection, mammalian expression, recombinational cloning, with tag/fusion/marker
Author Name: Invitrogen
Publications: None
Vector Map:         Vector Sequence:
Sequence:

 



Vector Features:

Type Name Description Start Position End Position
bacterial origin pUC ori pUC origin of replication 7733 8406
death cassette ccdB ccdB death cassette (removed when insert is present) 3767 3976
packaging signal HIV-1 psi HIV-1 psi packaging signal 521 565
poly-A signal SV40 SV40 polyadenylation signal 5639 5770
promoter RSV RSV/5' LTR hybrid promoter 1 410
promoter CMV CMV promoter driving gene of interest 1809 2392
promoter PGK PGK promoter to drive selection marker in mammalian cells 4266 4771
recombination site attR2 attR2 recombinational cloning site 4127 4142
recombination site attR1 attR1 recombinational cloning site 2440 2456
response element HIV-1 RRE HIV-1 Rev response element (RRE) 1075 1308
selectable marker AmpR Ampicillin resistance gene for selection in bacteria 6728 7588
selectable marker BlastR Blasticidin resistance gene in mammalian cells 4849 5249
selectable marker CmR chloramphenicol resistance marker (removed in plasmids with insert) 2673 3329
splice acceptor site 3' splice acceptor two 3' splice acceptors at 1656 and 1684 1656 1656
splice donor site 5' splice donor 5' splice donor 520 520
tag V5 C-terminal V5 epitope tag 4197 4238

 

  • Gene
  • Protein
  • Clone
  • Organism
  • Genome
  • Reagents

Gene

 

Gene Symbol : ZNHIT6
Symbol Nomenclature : ZNHIT6
SYNONYM : BCD1; C1orf181; NY-BR-75
Designation : box C/D snoRNA essential 1 homolog|box C/D snoRNA protein 1|serologically defined breast cancer antigen NY-BR-75|zinc finger HIT domain-containing protein 6|zinc finger, HIT type 6
Full Nomenclature : zinc finger, HIT-type containing 6
GENEID : 54680
HGNC : 26089
Target GenBank: BC110898

 

Reference Sequence Alignment

  

HsCD00329679      ATGGAGTTTGCTGCTGAAAATGAAGGGAAGTCTGGGGGAGGTCTCCACAGCGTAGCTGAG
NM_017953.3       ATGGAGTTTGCTGCTGAAAATGAAGGGAAGTCTGGGGGAGGTCTCCACAGCGTAGCTGAG
                  ************************************************************

HsCD00329679      GGGGTGCGGCTAAGTCCAGAGCCTGGCAGGGAGGGAGTAAGGGACTTAGCAGGGGCGGAG
NM_017953.3       GGGGTGCGGCTAAGTCCAGAGCCTGGCAGGGAGGGAGTAAGGGACTTAGCAGGGGCGGAG
                  ************************************************************

HsCD00329679      GAGTTCGGCGGCGGAGAGGAGGGGACAGGGCTGACAGGGATAAAGGAGATAGGGGATGGA
NM_017953.3       GAGTTCGGCGGCGGAGAGGAGGGGACAGGGCTGACAGGGATAAAGGAGATAGGGGATGGA
                  ************************************************************

HsCD00329679      GAGGAAGGAAGTGGACAAAGGCCAGAGGAAATACCGATGGACCTAACGGTAGTGAAGCAG
NM_017953.3       GAGGAAGGAAGTGGACAAAGGCCAGAGGAAATACCGATGGACCTAACGGTAGTGAAGCAG
                  ************************************************************

HsCD00329679      GAAATTATAGACTGGCCAGGTACAGAAGGCAGGTTGGCTGGCCAGTGGGTAGAACAGGAG
NM_017953.3       GAAATTATAGACTGGCCAGGTACAGAAGGCAGGTTGGCTGGCCAGTGGGTAGAACAGGAG
                  ************************************************************

HsCD00329679      GTGGAGGATAGGCCTGAGGTGAAGGATGAGAACGCAGGCGTATTGGAGGTGAAGCAGGAG
NM_017953.3       GTGGAGGATAGGCCTGAGGTGAAGGATGAGAACGCAGGCGTATTGGAGGTGAAGCAGGAG
                  ************************************************************

HsCD00329679      ACGGATAGTAGTTTAGTGGTAAAAGAAGCGAAGGTGGGTGAACCAGAGGTAAAGGAAGAG
NM_017953.3       ACGGATAGTAGTTTAGTGGTAAAAGAAGCGAAGGTGGGTGAACCAGAGGTAAAGGAAGAG
                  ************************************************************

HsCD00329679      AAGGTAAAGGAAGAGGTAATGGACTGGTCAGAAGTGAAGGAAGAGAAGGATAACTTGGAG
NM_017953.3       AAGGTAAAGGAAGAGGTAATGGACTGGTCAGAAGTGAAGGAAGAGAAGGATAACTTGGAG
                  ************************************************************

HsCD00329679      ATAAAACAGGAGGAGAAGTTTGTTGGTCAATGCATAAAAGAGGAATTGATGCATGGAGAG
NM_017953.3       ATAAAACAGGAGGAGAAGTTTGTTGGTCAATGCATAAAAGAGGAATTGATGCATGGAGAG
                  ************************************************************

HsCD00329679      TGTGTAAAAGAAGAGAAGGATTTCCTGAAGAAAGAAATCGTGGATGATACAAAGGTGAAA
NM_017953.3       TGTGTAAAAGAAGAGAAGGATTTCCTGAAGAAAGAAATCGTGGATGATACAAAGGTGAAA
                  ************************************************************

HsCD00329679      GAAGAGCCTCCGATAAATCACCCGGTGGGCTGCAAGCGGAAACTGGCCATGTCAAGGTGT
NM_017953.3       GAAGAGCCTCCGATAAATCACCCGGTGGGCTGCAAGCGGAAACTGGCCATGTCAAGGTGT
                  ************************************************************

HsCD00329679      GAGACTTGTGGTACAGAAGAAGCAAAGTACAGATGTCCACGTTGTATGCGATATTCCTGC
NM_017953.3       GAGACTTGTGGTACAGAAGAAGCAAAGTACAGATGTCCACGTTGTATGCGATATTCCTGC
                  ************************************************************

HsCD00329679      AGTTTGCCCTGTGTAAAGAAACACAAAGCAGAACTGACATGTAATGGAGTTCGAGATAAA
NM_017953.3       AGTTTGCCCTGTGTAAAGAAACACAAAGCAGAACTGACATGTAATGGAGTTCGAGATAAA
                  ************************************************************

HsCD00329679      ACTGCATACATTTCAATACAACAGTTTACTGAAATGAATCTCCTAAGTGATTATCGATTT
NM_017953.3       ACTGCATACATTTCAATACAACAGTTTACTGAAATGAATCTCCTAAGTGATTATCGATTT
                  ************************************************************

HsCD00329679      TTGGAAGATGTGGCAAGAACAGCGGACCATATTTCTAGAGATGCTTTTTTGAAGAGACCA
NM_017953.3       TTGGAAGATGTGGCAAGAACAGCGGACCATATTTCTAGAGATGCTTTTTTGAAGAGACCA
                  ************************************************************

HsCD00329679      ATAAGCAATAAATATATGTACTTTATGAAAAATCGTGCCCGGAGGCAAGGTATTAACTTA
NM_017953.3       ATAAGCAATAAATATATGTACTTTATGAAAAATCGTGCCCGGAGGCAAGGTATTAACTTA
                  ************************************************************

HsCD00329679      AAACTTCTACCCAATGGATTCACCAAGAGGAAGGAGAATTCAACCTTTTTTGATAAGAAA
NM_017953.3       AAACTTCTACCCAATGGATTCACCAAGAGGAAGGAGAATTCAACCTTTTTTGATAAGAAA
                  ************************************************************

HsCD00329679      AAACAACAGTTTTGTTGGCATGTGAAGCTCCAGTTTCCTCAAAGTCAAGCTGAGTACATA
NM_017953.3       AAACAACAGTTTTGTTGGCATGTGAAGCTCCAGTTTCCTCAAAGTCAAGCTGAGTACATA
                  ************************************************************

HsCD00329679      GAAAAAAGAGTACCAGATGATAAAACTATTAATGAAATCCTAAAACCTTACATTGATCCT
NM_017953.3       GAAAAAAGAGTACCAGATGATAAAACTATTAATGAAATCCTAAAACCTTACATTGATCCT
                  ************************************************************

HsCD00329679      GAAAAGTCTGATCCTGTAATTCGTCAAAGGTTGAAAGCCTACATTCGCTCTCAGACTGGG
NM_017953.3       GAAAAGTCTGATCCTGTAATTCGTCAAAGGTTGAAAGCCTACATTCGCTCTCAGACTGGG
                  ************************************************************

HsCD00329679      GTTCAGATTTTAATGAAGATTGAATATATGCAGCAAAATTTAGTAAGATATTATGAACTA
NM_017953.3       GTTCAGATTTTAATGAAGATTGAATATATGCAGCAAAATTTAGTAAGATATTATGAACTA
                  ************************************************************

HsCD00329679      GATCCTTATAAAAGTCTCCTAGACAATTTGAGGAACAAAGTGATCATTGAGTATCCAACA
NM_017953.3       GATCCTTATAAAAGTCTCCTAGACAATTTGAGGAACAAAGTGATCATTGAGTATCCAACA
                  ************************************************************

HsCD00329679      TTACATGTGGTATTGAAAGGATCCAATAATGACATGAAAGTTCTTCACCAAGTGAAGAGT
NM_017953.3       TTACATGTGGTATTGAAAGGATCCAATAATGACATGAAAGTTCTTCACCAAGTGAAGAGT
                  ************************************************************

HsCD00329679      GAATCTACCAAGAACGTTGGCAATGAAAATTTG
NM_017953.3       GAATCTACCAAGAACGTTGGCAATGAAAATTGA
                  ******************************* .

Protein

UniProt : B4DP13
UniProt : Q9NWK9
HPRD : 08630

Clone

Cloning Information : 268136
HIP Master Clone ID : 193176
Original Clone ID : FLH268136.01L

Organism


TAX_ID : 9606
Species Specific ID: 54680

Genome

Chromosome : 1
Map Location : 1p22.3
Ensembl : ENSG00000117174

Reagents

Labome : ZNHIT6-antibody