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KLHDC5 (Homo sapiens) in pLenti6.2/V5-DEST (V5-tagged lentiviral mammalian expression vector)

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Explanation of Terms


Gene: Gene Symbol:  KLHL42
Gene Name:  kelch-like family member 42
Sequence             
Original Clone ID: FLH268139.01L
Keyword: None
Species: Homo sapiens
Type: cDNA
Vector Name: pLenti6.2/V5-DEST               Format:  FUSION
Source: HIP
Description: None
Comments: None
Mutation/
Discrepancy :
No / No
Publications: None
Authors:
HsCD00329682

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AVAILABLE
No restriction
Special MTA: None

 

Insert sequence: 1518nts         Open reading frame : 1 to 1518
Reference Sequence Alignment

ATGTCGGCCGAGGAGATGGTGCAGATCCGCCTGGAGGACCGCTGCTACCCGGTGAGCAAG
AGGAAGCTCATCGAGCAGAGCGACTACTTCCGCGCCCTCTACCGCTCCGGCATGCGCGAG
GCCCTGAGCCAGGAGGCCGGCGGCCCGGAGGTGCAGCAGCTGCGCGGCCTCAGCGCGCCG
GGCCTGCGGCTGGTACTGGACTTCATCAACGCCGGCGGGGCCCGCGAAGGCTGGCTCCTG
GGCCCGCGCGGGGAAAAGGGCGGCGGGGTGGACGAGGACGAGGAGATGGATGAGGTGAGC
CTGCTGTCCGAGCTGGTGGAGGCGGCCTCCTTCCTGCAGGTCACGTCCCTGCTGCAGCTG
CTGCTGTCCCAGGTGCGGCTCAATAACTGCCTGGAGATGTACCGCCTGGCGCAGGTGTAC
GGGCTGCCCGACCTGCAGGAGGCCTGCCTGCGCTTCATGGTCGTCCACTTCCACGAGGTG
CTGTGCAAGCCCCAGTTCCACCTCCTGGGGTCTCCTCCCCAAGCTCCAGGGGATGTCAGC
CTGAAGCAGAGGCTGAGGGAGGCCCGGATGACTGGGACTCCTGTCCTCGTGGCCCTCGGG
GACTTCCTGGGGGGACCCCTGGCCCCTCACCCCTACCAGGGGGAGCCCCCGTCCATGCTC
AGGTACGAGGAGATGACTGAGCGTTGGTTCCCGCTGGCCAACAACCTTCCTCCCGACCTG
GTCAATGTCAGGGGCTATGGGTCTGCCATCCTGGACAACTACCTCTTCATAGTGGGCGGG
TACAGGATCACTAGCCAGGAGATCTCCGCTGCGCATTCCTACAACCCCAGCACCAACGAG
TGGCTCCAGGTGGCCTCCATGAACCAGAAGAGGTCTAACTTCAAACTTGTGGCTGTTAAT
TCAAAACTCTATGCCATCGGAGGGCAGGCCGTTTCTAACGTTGAGTGTTACAACCCCGAG
CAGGATGCGTGGAATTTTGTGGCGCCCTTACCCAATCCTCTGGCTGAGTTCTCTGCCTGT
GAGTGTAAGGGAAAAATTTATGTCATTGGAGGATACACTACCAGAGACCGGAACATGAAC
ATTTTGCAGTACTGCCCCTCTTCCGACATGTGGACGCTCTTTGAAACATGTGACGTCCAC
ATTCGCAAGCAGCAGATGGTGTCTGTGGAAGAGACCATCTACATCGTGGGGGGGTGTCTC
CACGAGCTGGGGCCCAACCGCAGGAGCAGCCAGAGCGAGGACATGCTCACCGTGCAGTCC
TACAACACCGTCACCCGCCAGTGGCTCTACCTCAAGGAGAACACGTCCAAATCGGGTCTT
AACTTGACTTGTGCGCTCCATAACGACGGCATCTACATCATGAGCAGAGACGTCACCCTG
TCGACCAGCTTGGAACACCGAGTGTTCCTCAAGTACAACATCTTTTCAGATAGTTGGGAA
GCATTTCGGCGTTTTCCAGCTTTTGGACATAACTTGCTGGTTTCTTCTCTTTATCTGCCC
AATAAAGCAGAAACATTG


Coding Sequence Details
Insert Sequence Verified?: N Verification Method: Insert was fully sequenced in parent vector
Reference Sequence Annotations:
End on reference sequence 1566
Start on reference sequence 49
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
5p GTACAAAAAAGCAGGCTCCACC(atg-goi)

3' Linker Sequence:
5p (goi-stop)GACCCAGCTTTCTTGTAC

 

 Recommended Growth Condition:

Distributed in bacterial strain : DH5-alpha T1 phage resistant
Antibiotic Selection: Host Type:  bacterial    Marker:  ampicillin
Host Type:  mammalian    Marker:  blasticidin
Bacterial Selection Condition:  100 ug/mL ampicillin
Growth Condition:  Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:  Commonly used conditions for ampicillin resistant plasmid clones.
Protein Expression Results: None
Recommended expression in: Not Applicable
       DyNA Vector Map

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Vector Name: pLenti6.2/V5-DEST

pLenti6.2V5-DEST in advanced viewed

Synonyms: pLenti6.2/V5-DEST
Sequencing Primer: Forward:  CMV forward
Reverse:  V5 reverse
Description: Lentiviral mammalian expression vector;CMV promoter and C-terminal V5 tag; ampicillin resistance in bacteria; blasticidin resistance in mammalian cells; Gateway recombinational cloning.
Comments: None
Size (bp): 8813
Parent Vector: None
Empty Vector: None
Properties: Gateway, acceptor (destination), lentiviral infection, mammalian expression, recombinational cloning, with tag/fusion/marker
Author Name: Invitrogen
Publications: None
Vector Map:         Vector Sequence:
Sequence:

 



Vector Features:

Type Name Description Start Position End Position
bacterial origin pUC ori pUC origin of replication 7733 8406
death cassette ccdB ccdB death cassette (removed when insert is present) 3767 3976
packaging signal HIV-1 psi HIV-1 psi packaging signal 521 565
poly-A signal SV40 SV40 polyadenylation signal 5639 5770
promoter RSV RSV/5' LTR hybrid promoter 1 410
promoter CMV CMV promoter driving gene of interest 1809 2392
promoter PGK PGK promoter to drive selection marker in mammalian cells 4266 4771
recombination site attR2 attR2 recombinational cloning site 4127 4142
recombination site attR1 attR1 recombinational cloning site 2440 2456
response element HIV-1 RRE HIV-1 Rev response element (RRE) 1075 1308
selectable marker AmpR Ampicillin resistance gene for selection in bacteria 6728 7588
selectable marker BlastR Blasticidin resistance gene in mammalian cells 4849 5249
selectable marker CmR chloramphenicol resistance marker (removed in plasmids with insert) 2673 3329
splice acceptor site 3' splice acceptor two 3' splice acceptors at 1656 and 1684 1656 1656
splice donor site 5' splice donor 5' splice donor 520 520
tag V5 C-terminal V5 epitope tag 4197 4238

 

  • Gene
  • Protein
  • Clone
  • Organism
  • Genome
  • Reagents

Gene

 

Gene Symbol : KLHL42
Symbol Nomenclature : KLHL42
SYNONYM : Ctb9; KLHDC5
Designation : cullin-3-binding protein 9|kelch domain containing 5|kelch domain-containing protein 5|kelch-like family, member 42|kelch-like protein 42
Full Nomenclature : kelch-like family member 42
GENEID : 57542
HGNC : 29252
Vega : OTTHUMG00000169217
Target GenBank: BC108669

 

Reference Sequence Alignment

  

HsCD00329682      ATGTCGGCCGAGGAGATGGTGCAGATCCGCCTGGAGGACCGCTGCTACCCGGTGAGCAAG
NM_020782.1       ATGTCGGCCGAGGAGATGGTGCAGATCCGCCTGGAGGACCGCTGCTACCCGGTGAGCAAG
                  ************************************************************

HsCD00329682      AGGAAGCTCATCGAGCAGAGCGACTACTTCCGCGCCCTCTACCGCTCCGGCATGCGCGAG
NM_020782.1       AGGAAGCTCATCGAGCAGAGCGACTACTTCCGCGCCCTCTACCGCTCCGGCATGCGCGAG
                  ************************************************************

HsCD00329682      GCCCTGAGCCAGGAGGCCGGCGGCCCGGAGGTGCAGCAGCTGCGCGGCCTCAGCGCGCCG
NM_020782.1       GCCCTGAGCCAGGAGGCCGGCGGCCCGGAGGTGCAGCAGCTGCGCGGCCTCAGCGCGCCG
                  ************************************************************

HsCD00329682      GGCCTGCGGCTGGTACTGGACTTCATCAACGCCGGCGGGGCCCGCGAAGGCTGGCTCCTG
NM_020782.1       GGCCTGCGGCTGGTGCTGGACTTCATCAACGCCGGCGGGGCCCGCGAAGGCTGGCTCCTG
                  **************.*********************************************

HsCD00329682      GGCCCGCGCGGGGAAAAGGGCGGCGGGGTGGACGAGGACGAGGAGATGGATGAGGTGAGC
NM_020782.1       GGCCCGCGCGGGGAAAAGGGCGGCGGGGTGGACGAGGACGAGGAGATGGATGAGGTGAGC
                  ************************************************************

HsCD00329682      CTGCTGTCCGAGCTGGTGGAGGCGGCCTCCTTCCTGCAGGTCACGTCCCTGCTGCAGCTG
NM_020782.1       CTGCTGTCCGAGCTGGTGGAGGCGGCCTCCTTCCTGCAGGTCACGTCCCTGCTGCAGCTG
                  ************************************************************

HsCD00329682      CTGCTGTCCCAGGTGCGGCTCAATAACTGCCTGGAGATGTACCGCCTGGCGCAGGTGTAC
NM_020782.1       CTGCTGTCCCAGGTGCGGCTCAATAACTGCCTGGAGATGTACCGCCTGGCGCAGGTGTAC
                  ************************************************************

HsCD00329682      GGGCTGCCCGACCTGCAGGAGGCCTGCCTGCGCTTCATGGTCGTCCACTTCCACGAGGTG
NM_020782.1       GGGCTGCCCGACCTGCAGGAGGCCTGCCTGCGCTTCATGGTCGTCCACTTCCACGAGGTG
                  ************************************************************

HsCD00329682      CTGTGCAAGCCCCAGTTCCACCTCCTGGGGTCTCCTCCCCAAGCTCCAGGGGATGTCAGC
NM_020782.1       CTGTGCAAGCCCCAGTTCCACCTCCTGGGGTCTCCTCCCCAAGCTCCAGGGGATGTCAGC
                  ************************************************************

HsCD00329682      CTGAAGCAGAGGCTGAGGGAGGCCCGGATGACTGGGACTCCTGTCCTCGTGGCCCTCGGG
NM_020782.1       CTGAAGCAGAGGCTGAGGGAGGCCCGGATGACTGGGACTCCTGTCCTCGTGGCCCTCGGG
                  ************************************************************

HsCD00329682      GACTTCCTGGGGGGACCCCTGGCCCCTCACCCCTACCAGGGGGAGCCCCCGTCCATGCTC
NM_020782.1       GACTTCCTGGGGGGACCCCTGGCCCCTCACCCCTACCAGGGGGAGCCCCCGTCCATGCTC
                  ************************************************************

HsCD00329682      AGGTACGAGGAGATGACTGAGCGTTGGTTCCCGCTGGCCAACAACCTTCCTCCCGACCTG
NM_020782.1       AGGTACGAGGAGATGACTGAGCGTTGGTTCCCGCTGGCCAACAACCTTCCTCCCGACCTG
                  ************************************************************

HsCD00329682      GTCAATGTCAGGGGCTATGGGTCTGCCATCCTGGACAACTACCTCTTCATAGTGGGCGGG
NM_020782.1       GTCAATGTCAGGGGCTATGGGTCTGCCATCCTGGACAACTACCTCTTCATAGTGGGCGGG
                  ************************************************************

HsCD00329682      TACAGGATCACTAGCCAGGAGATCTCCGCTGCGCATTCCTACAACCCCAGCACCAACGAG
NM_020782.1       TACAGGATCACTAGCCAGGAGATCTCCGCTGCGCATTCCTACAACCCCAGCACCAACGAG
                  ************************************************************

HsCD00329682      TGGCTCCAGGTGGCCTCCATGAACCAGAAGAGGTCTAACTTCAAACTTGTGGCTGTTAAT
NM_020782.1       TGGCTCCAGGTGGCCTCCATGAACCAGAAGAGGTCTAACTTCAAACTTGTGGCTGTTAAT
                  ************************************************************

HsCD00329682      TCAAAACTCTATGCCATCGGAGGGCAGGCCGTTTCTAACGTTGAGTGTTACAACCCCGAG
NM_020782.1       TCAAAACTCTATGCCATCGGAGGGCAGGCCGTTTCTAACGTTGAGTGTTACAACCCCGAG
                  ************************************************************

HsCD00329682      CAGGATGCGTGGAATTTTGTGGCGCCCTTACCCAATCCTCTGGCTGAGTTCTCTGCCTGT
NM_020782.1       CAGGATGCGTGGAATTTTGTGGCGCCCTTACCCAATCCTCTGGCTGAGTTCTCTGCCTGT
                  ************************************************************

HsCD00329682      GAGTGTAAGGGAAAAATTTATGTCATTGGAGGATACACTACCAGAGACCGGAACATGAAC
NM_020782.1       GAGTGTAAGGGAAAAATTTATGTCATTGGAGGATACACTACCAGAGACCGGAACATGAAC
                  ************************************************************

HsCD00329682      ATTTTGCAGTACTGCCCCTCTTCCGACATGTGGACGCTCTTTGAAACATGTGACGTCCAC
NM_020782.1       ATTTTGCAGTACTGCCCCTCTTCCGACATGTGGACGCTCTTTGAAACATGTGACGTCCAC
                  ************************************************************

HsCD00329682      ATTCGCAAGCAGCAGATGGTGTCTGTGGAAGAGACCATCTACATCGTGGGGGGGTGTCTC
NM_020782.1       ATTCGCAAGCAGCAGATGGTGTCTGTGGAAGAGACCATCTACATCGTGGGGGGGTGTCTC
                  ************************************************************

HsCD00329682      CACGAGCTGGGGCCCAACCGCAGGAGCAGCCAGAGCGAGGACATGCTCACCGTGCAGTCC
NM_020782.1       CACGAGCTGGGGCCCAACCGCAGGAGCAGCCAGAGCGAGGACATGCTCACCGTGCAGTCC
                  ************************************************************

HsCD00329682      TACAACACCGTCACCCGCCAGTGGCTCTACCTCAAGGAGAACACGTCCAAATCGGGTCTT
NM_020782.1       TACAACACCGTCACCCGCCAGTGGCTCTACCTCAAGGAGAACACGTCCAAATCGGGTCTT
                  ************************************************************

HsCD00329682      AACTTGACTTGTGCGCTCCATAACGACGGCATCTACATCATGAGCAGAGACGTCACCCTG
NM_020782.1       AACTTGACTTGTGCGCTCCATAACGACGGCATCTACATCATGAGCAGAGACGTCACCCTG
                  ************************************************************

HsCD00329682      TCGACCAGCTTGGAACACCGAGTGTTCCTCAAGTACAACATCTTTTCAGATAGTTGGGAA
NM_020782.1       TCGACCAGCTTGGAACACCGAGTGTTCCTCAAGTACAACATCTTTTCAGATAGTTGGGAA
                  ************************************************************

HsCD00329682      GCATTTCGGCGTTTTCCAGCTTTTGGACATAACTTGCTGGTTTCTTCTCTTTATCTGCCC
NM_020782.1       GCATTTCGGCGTTTTCCAGCTTTTGGACATAACTTGCTGGTTTCTTCTCTTTATCTGCCC
                  ************************************************************

HsCD00329682      AATAAAGCAGAAACATTG
NM_020782.1       AATAAAGCAGAAACATGA
                  **************** .

Protein

SMART domain : Kelch :
UniProt : Q9P2K6
UniProt : B2RNT7
HPRD : 17237

Clone

Cloning Information : 268139
HIP Master Clone ID : 193181
Original Clone ID : FLH268139.01L

Organism


TAX_ID : 9606
Species Specific ID: 57542

Genome

Chromosome : 12
Map Location : 12p11.22
Ensembl : ENSG00000087448

Reagents

Labome : KLHL42-antibody