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CD3EAP (Homo sapiens) in pLenti6.2/V5-DEST (V5-tagged lentiviral mammalian expression vector)

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Explanation of Terms


Gene: Gene Symbol:  CD3EAP
Gene Name:  CD3e molecule, epsilon associated protein
Sequence             
Original Clone ID: FLH268141.01L
Keyword: None
Species: Homo sapiens
Type: cDNA
Vector Name: pLenti6.2/V5-DEST               Format:  FUSION
Source: HIP
Description: None
Comments: None
Mutation/
Discrepancy :
No / No
Publications: None
Authors:
HsCD00329684

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AVAILABLE
No restriction
Special MTA: None

 

Insert sequence: 1530nts         Open reading frame : 1 to 1530
Reference Sequence Alignment

ATGGAGGAGCCCCAGGCCGGCGATGCTGCTCGGTTCTCTTGTCCCCCCAACTTTACCGCG
AAGCCCCCAGCCTCAGAGTCCCCTCGTTTCTCCTTGGAGGCGCTGACGGGTCCAGATACG
GAGCTGTGGCTTATTCAGGCCCCTGCAGACTTTGCCCCAGAATGCTTCAATGGGCGGCAT
GTGCCTCTCTCTGGCTCCCAGATCGTCAAGGGCAAATTGGCAGGCAAGCGGCACCGCTAT
CGAGTCCTCAGCAGCTGTCCCCAAGCTGGAGAAGCGACCCTGCTGGCCCCCTCAACGGAG
GCAGGAGGTGGACTCACCTGTGCCTCAGCCCCCCAGGGCACCCTAAGGATCCTTGAGGGT
CCCCAGCAATCCCTGTCAGGGAGCCCTCTGCAGCCCATCCCAGCAAGTCCCCCACCACAG
ATCCCTCCTGGCCTGAGGCCTCGGTTCTGTGCCTTTGGGGGCAACCCACCAGTCACAGGG
CCTAGGTCAGCCTTGGCCCCCAACCTGCTCACCTCAGGGAAGAAGAAAAAGGAGATGCAG
GTGACAGAGGCCCCAGTCACTCAGGAGGCAGTGAATGGGCACGGGGCCCTGGAGGTGGAC
ATGGCTTTGGGGTCGCCAGAAATGGATGTGCGGAAGAAGAAGAAGAAAAAAAATCAGCAG
CTGAAAGAACCAGAGGCAGCAGGGCCTGTGGGGACAGAGCCCACAGTGGAGACACTGGAG
CCTCTGGGAGTGCTGTTCCCGTCCACCACCAAGAAGAGGAAGAAGCCCAAAGGGAAAGAA
ACCTTCGAGCCAGAAGACAAGACAGTGAAGCAGGAACAGATTAACACTGAGCCTCTAGAA
GACACAGTCCTGTCCCCGACCAAAAAGAGAAAGAGGCAAAAGGGGACGGAAGGGATGGAG
CCAGAGGAGGGGGTGACAGTTGAGTCTCAGCCACAGGTGAAGGTGGAGCCACTGGAGGAA
GCCATCCCTCTGCCCCCTACGAAGAAGAGGAAAAAAGAAAAGGGACAGATGGCAATGATG
GAGCCAGGGACGGAGGCGATGGAGCCAGTGGAGCCGGAGATGAAGCCTCTGGAGTCCCCA
GGGGGGACCATGGCGCCTCAACAGCCAGAAGGAGCGAAGCCTCAGGCCCAGGCAGCTCTG
GCAGCTCCCAAAAAGAAGACGAAGAAAGAAAAACAGCAAGATGCCACAGTGGAGCCAGAG
ACAGAGGTGGTGGGGCCTGAGCTGCCGGATGACCTTGAGCCTCAGGCAGCTCCCACATCC
ACCAAGAAGAAGAAGAAGAAAGAGAGAGGTCACACAGTGACTGAGCCAATTCAGCCACTA
GAGCCTGAACTGCCAGGGGAGGGACAGCCTGAAGCCAGGGCAACTCCGGGATCCACCAAG
AAGAGGAAGAAGCAGAGTCAGGAAAGCCGGATGCCAGAGACAGTGCCCCAAGAGGAGATG
CCAGGGCCGCCACTGAATTCAGAGTCTGGGGAGGAGGCTCCCACAGGCCGGGACAAGAAG
CGGAAGAAGCAGCAGCAGCAGCCTGTGTTG


Coding Sequence Details
Insert Sequence Verified?: N Verification Method: Insert was fully sequenced in parent vector
Reference Sequence Annotations:
End on reference sequence 1783
Start on reference sequence 254
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
5p GTACAAAAAAGCAGGCTCCACC(atg-goi)

3' Linker Sequence:
5p (goi-stop)GACCCAGCTTTCTTGTAC

 

 Recommended Growth Condition:

Distributed in bacterial strain : DH5-alpha T1 phage resistant
Antibiotic Selection: Host Type:  bacterial    Marker:  ampicillin
Host Type:  mammalian    Marker:  blasticidin
Bacterial Selection Condition:  100 ug/mL ampicillin
Growth Condition:  Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:  Commonly used conditions for ampicillin resistant plasmid clones.
Protein Expression Results: None
Recommended expression in: Not Applicable
       DyNA Vector Map

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Vector Name: pLenti6.2/V5-DEST

pLenti6.2V5-DEST in advanced viewed

Synonyms: pLenti6.2/V5-DEST
Sequencing Primer: Forward:  CMV forward
Reverse:  V5 reverse
Description: Lentiviral mammalian expression vector;CMV promoter and C-terminal V5 tag; ampicillin resistance in bacteria; blasticidin resistance in mammalian cells; Gateway recombinational cloning.
Comments: None
Size (bp): 8813
Parent Vector: None
Empty Vector: None
Properties: Gateway, acceptor (destination), lentiviral infection, mammalian expression, recombinational cloning, with tag/fusion/marker
Author Name: Invitrogen
Publications: None
Vector Map:         Vector Sequence:
Sequence:

 



Vector Features:

Type Name Description Start Position End Position
bacterial origin pUC ori pUC origin of replication 7733 8406
death cassette ccdB ccdB death cassette (removed when insert is present) 3767 3976
packaging signal HIV-1 psi HIV-1 psi packaging signal 521 565
poly-A signal SV40 SV40 polyadenylation signal 5639 5770
promoter RSV RSV/5' LTR hybrid promoter 1 410
promoter CMV CMV promoter driving gene of interest 1809 2392
promoter PGK PGK promoter to drive selection marker in mammalian cells 4266 4771
recombination site attR2 attR2 recombinational cloning site 4127 4142
recombination site attR1 attR1 recombinational cloning site 2440 2456
response element HIV-1 RRE HIV-1 Rev response element (RRE) 1075 1308
selectable marker AmpR Ampicillin resistance gene for selection in bacteria 6728 7588
selectable marker BlastR Blasticidin resistance gene in mammalian cells 4849 5249
selectable marker CmR chloramphenicol resistance marker (removed in plasmids with insert) 2673 3329
splice acceptor site 3' splice acceptor two 3' splice acceptors at 1656 and 1684 1656 1656
splice donor site 5' splice donor 5' splice donor 520 520
tag V5 C-terminal V5 epitope tag 4197 4238

 

  • Gene
  • Protein
  • Clone
  • Organism
  • Genome
  • Reagents

Gene

 

Gene Symbol : CD3EAP
Symbol Nomenclature : CD3EAP
SYNONYM : ASE-1; ASE1; CAST; PAF49
Designation : A34.5|CD3 epsilon associated protein|CD3-epsilon-associated protein|CD3E-associated protein|CD3e antigen, epsilon polypeptide associated protein|DNA-directed RNA polymerase I subunit RPA34|RNA polymerase I-associated factor PAF49|antisense to ERCC 1|antisense to ERCC-1 protein
Full Nomenclature : CD3e molecule, epsilon associated protein
GENEID : 10849
HGNC : 24219
MIM : 107325
Vega : OTTHUMG00000182120
Target GenBank: BC108889

 

Reference Sequence Alignment

  

HsCD00329684        ATGGAGGAGCCCCAGGCCGGCG---------ATGCTGCTCGGTTCTCTTGTCCCCCCAAC
XM_005258425.1      ATGGAGGAGCCCCAGGCCG---GCGGTGAGGATGCTGCTCGGTTCTCTTGTCCCCCCAAC
NM_012099.1         ATGGAGGAGCCCCAGGCCG---GCG------ATGCTGCTCGGTTCTCTTGTCCCCCCAAC
                    *******************            *****************************

HsCD00329684        TTTACCGCGAAGCCCCCAGCCTCAGAGTCCCCTCGTTTCTCCTTGGAGGCGCTGACGGGT
XM_005258425.1      TTTACCGCGAAGCCCCCAGCCTCAGAGTCCCCTCGTTTCTCCTTGGAGGCGCTGACGGGT
NM_012099.1         TTTACCGCGAAGCCCCCAGCCTCAGAGTCCCCTCGTTTCTCCTTGGAGGCGCTGACGGGT
                    ************************************************************

HsCD00329684        CCAGATACGGAGCTGTGGCTTATTCAGGCCCCTGCAGACTTTGCCCCAGAATGCTTCAAT
XM_005258425.1      CCAGATACGGAGCTGTGGCTTATTCAGGCCCCTGCAGACTTTGCCCCAGAATGCTTCAAT
NM_012099.1         CCAGATACGGAGCTGTGGCTTATTCAGGCCCCTGCAGACTTTGCCCCAGAATGCTTCAAT
                    ************************************************************

HsCD00329684        GGGCGGCATGTGCCTCTCTCTGGCTCCCAGATCGTCAAGGGCAAATTGGCAGGCAAGCGG
XM_005258425.1      GGGCGGCATGTGCCTCTCTCTGGCTCCCAGATCGTCAAGGGCAAATTGGCAGGCAAGCGG
NM_012099.1         GGGCGGCATGTGCCTCTCTCTGGCTCCCAGATCGTCAAGGGCAAATTGGCAGGCAAGCGG
                    ************************************************************

HsCD00329684        CACCGCTATCGAGTCCTCAGCAGCTGTCCCCAAGCTGGAGAAGCGACCCTGCTGGCCCCC
XM_005258425.1      CACCGCTATCGAGTCCTCAGCAGCTGTCCCCAAGCTGGAGAAGCGACCCTGCTGGCCCCC
NM_012099.1         CACCGCTATCGAGTCCTCAGCAGCTGTCCCCAAGCTGGAGAAGCGACCCTGCTGGCCCCC
                    ************************************************************

HsCD00329684        TCAACGGAGGCAGGAGGTGGACTCACCTGTGCCTCAGCCCCCCAGGGCACCCTAAGGATC
XM_005258425.1      TCAACGGAGGCAGGAGGTGGACTCACCTGTGCCTCAGCCCCCCAGGGCACCCTAAGGATC
NM_012099.1         TCAACGGAGGCAGGAGGTGGACTCACCTGTGCCTCAGCCCCCCAGGGCACCCTAAGGATC
                    ************************************************************

HsCD00329684        CTTGAGGGTCCCCAGCAATCCCTGTCAGGGAGCCCTCTGCAGCCCATCCCAGCAAGTCCC
XM_005258425.1      CTTGAGGGTCCCCAGCAATCCCTGTCAGGGAGCCCTCTGCAGCCCATCCCAGCAAGTCCC
NM_012099.1         CTTGAGGGTCCCCAGCAATCCCTGTCAGGGAGCCCTCTGCAGCCCATCCCAGCAAGTCCC
                    ************************************************************

HsCD00329684        CCACCACAGATCCCTCCTGGCCTGAGGCCTCGGTTCTGTGCCTTTGGGGGCAACCCACCA
XM_005258425.1      CCACCACAGATCCCTCCTGGCCTGAGGCCTCGGTTCTGTGCCTTTGGGGGCAACCCACCA
NM_012099.1         CCACCACAGATCCCTCCTGGCCTGAGGCCTCGGTTCTGTGCCTTTGGGGGCAACCCACCA
                    ************************************************************

HsCD00329684        GTCACAGGGCCTAGGTCAGCCTTGGCCCCCAACCTGCTCACCTCAGGGAAGAAGAAAAAG
XM_005258425.1      GTCACAGGGCCTAGGTCAGCCTTGGCCCCCAACCTGCTCACCTCAGGGAAGAAGAAAAAG
NM_012099.1         GTCACAGGGCCTAGGTCAGCCTTGGCCCCCAACCTGCTCACCTCAGGGAAGAAGAAAAAG
                    ************************************************************

HsCD00329684        GAGATGCAGGTGACAGAGGCCCCAGTCACTCAGGAGGCAGTGAATGGGCACGGGGCCCTG
XM_005258425.1      GAGATGCAGGTGACAGAGGCCCCAGTCACTCAGGAGGCAGTGAATGGGCACGGGGCCCTG
NM_012099.1         GAGATGCAGGTGACAGAGGCCCCAGTCACTCAGGAGGCAGTGAATGGGCACGGGGCCCTG
                    ************************************************************

HsCD00329684        GAGGTGGACATGGCTTTGGGGTCGCCAGAAATGGATGTGCGGAAGAAGAAGAAGAAAAAA
XM_005258425.1      GAGGTGGACATGGCTTTGGGGTCGCCAGAAATGGATGTGCGGAAGAAGAAGAAGAAAAAA
NM_012099.1         GAGGTGGACATGGCTTTGGGGTCGCCAGAAATGGATGTGCGGAAGAAGAAGAAGAAAAAA
                    ************************************************************

HsCD00329684        AATCAGCAGCTGAAAGAACCAGAGGCAGCAGGGCCTGTGGGGACAGAGCCCACAGTGGAG
XM_005258425.1      AATCAGCAGCTGAAAGAACCAGAGGCAGCAGGGCCTGTGGGGACAGAGCCCACAGTGGAG
NM_012099.1         AATCAGCAGCTGAAAGAACCAGAGGCAGCAGGGCCTGTGGGGACAGAGCCCACAGTGGAG
                    ************************************************************

HsCD00329684        ACACTGGAGCCTCTGGGAGTGCTGTTCCCGTCCACCACCAAGAAGAGGAAGAAGCCCAAA
XM_005258425.1      ACACTGGAGCCTCTGGGAGTGCTGTTCCCGTCCACCACCAAGAAGAGGAAGAAGCCCAAA
NM_012099.1         ACACTGGAGCCTCTGGGAGTGCTGTTCCCGTCCACCACCAAGAAGAGGAAGAAGCCCAAA
                    ************************************************************

HsCD00329684        GGGAAAGAAACCTTCGAGCCAGAAGACAAGACAGTGAAGCAGGAACAGATTAACACTGAG
XM_005258425.1      GGGAAAGAAACCTTCGAGCCAGAAGACAAGACAGTGAAGCAGGAACAGATTAACACTGAG
NM_012099.1         GGGAAAGAAACCTTCGAGCCAGAAGACAAGACAGTGAAGCAGGAACAGATTAACACTGAG
                    ************************************************************

HsCD00329684        CCTCTAGAAGACACAGTCCTGTCCCCGACCAAAAAGAGAAAGAGGCAAAAGGGGACGGAA
XM_005258425.1      CCTCTAGAAGACACAGTCCTGTCCCCGACCAAAAAGAGAAAGAGGCAAAAGGGGACGGAA
NM_012099.1         CCTCTAGAAGACACAGTCCTGTCCCCGACCAAAAAGAGAAAGAGGCAAAAGGGGACGGAA
                    ************************************************************

HsCD00329684        GGGATGGAGCCAGAGGAGGGGGTGACAGTTGAGTCTCAGCCACAGGTGAAGGTGGAGCCA
XM_005258425.1      GGGATGGAGCCAGAGGAGGGGGTGACAGTTGAGTCTCAGCCACAGGTGAAGGTGGAGCCA
NM_012099.1         GGGATGGAGCCAGAGGAGGGGGTGACAGTTGAGTCTCAGCCACAGGTGAAGGTGGAGCCA
                    ************************************************************

HsCD00329684        CTGGAGGAAGCCATCCCTCTGCCCCCTACGAAGAAGAGGAAAAAAGAAAAGGGACAGATG
XM_005258425.1      CTGGAGGAAGCCATCCCTCTGCCCCCTACGAAGAAGAGGAAAAAAGAAAAGGGACAGATG
NM_012099.1         CTGGAGGAAGCCATCCCTCTGCCCCCTACGAAGAAGAGGAAAAAAGAAAAGGGACAGATG
                    ************************************************************

HsCD00329684        GCAATGATGGAGCCAGGGACGGAGGCGATGGAGCCAGTGGAGCCGGAGATGAAGCCTCTG
XM_005258425.1      GCAATGATGGAGCCAGGGACGGAGGCGATGGAGCCAGTGGAGCCGGAGATGAAGCCTCTG
NM_012099.1         GCAATGATGGAGCCAGGGACGGAGGCGATGGAGCCAGTGGAGCCGGAGATGAAGCCTCTG
                    ************************************************************

HsCD00329684        GAGTCCCCAGGGGGGACCATGGCGCCTCAACAGCCAGAAGGAGCGAAGCCTCAGGCCCAG
XM_005258425.1      GAGTCCCCAGGGGGGACCATGGCGCCTCAACAGCCAGAAGGAGCGAAGCCTCAGGCCCAG
NM_012099.1         GAGTCCCCAGGGGGGACCATGGCGCCTCAACAGCCAGAAGGAGCGAAGCCTCAGGCCCAG
                    ************************************************************

HsCD00329684        GCAGCTCTGGCAGCTCCCAAAAAGAAGACGAAGAAAGAAAAACAGCAAGATGCCACAGTG
XM_005258425.1      GCAGCTCTGGCAGCTCCCAAAAAGAAGACGAAGAAAGAAAAACAGCAAGATGCCACAGTG
NM_012099.1         GCAGCTCTGGCAGCTCCCAAAAAGAAGACGAAGAAAGAAAAACAGCAAGATGCCACAGTG
                    ************************************************************

HsCD00329684        GAGCCAGAGACAGAGGTGGTGGGGCCTGAGCTGCCGGATGACCTTGAGCCTCAGGCAGCT
XM_005258425.1      GAGCCAGAGACAGAGGTGGTGGGGCCTGAGCTGCCGGATGACCTTGAGCCTCAGGCAGCT
NM_012099.1         GAGCCAGAGACAGAGGTGGTGGGGCCTGAGCTGCCGGATGACCTTGAGCCTCAGGCAGCT
                    ************************************************************

HsCD00329684        CCCACATCCACCAAGAAGAAGAAGAAGAAAGAG---AGAGGTCACACAGTGACTGAGCCA
XM_005258425.1      CCCACATCCACCAAGAAGAAGAAGAAGAAGAAAGAGAGAGGTCACACAGTGACTGAGCCA
NM_012099.1         CCCACATCCACCAAGAAGAAGAAGAAGAAGAAAGAGAGAGGTCACACAGTGACTGAGCCA
                    *****************************..*.   ************************

HsCD00329684        ATTCAGCCACTAGAGCCTGAACTGCCAGGGGAGGGACAGCCTGAAGCCAGGGCAACTCCG
XM_005258425.1      ATTCAGCCACTAGAGCCTGAACTGCCAGGGGAGGGACAGCCTGAAGCCAGGGCAACTCCG
NM_012099.1         ATTCAGCCACTAGAGCCTGAACTGCCAGGGGAGGGACAGCCTGAAGCCAGGGCAACTCCG
                    ************************************************************

HsCD00329684        GGATCCACCAAGAAGAGGAAGAAGCAGAGTCAGGAAAGCCGGATGCCAGAGACAGTGCCC
XM_005258425.1      GGATCCACCAAGAAGAGGAAGAAGCAGAGTCAGGAAAGCCGGATGCCAGAGACAGTGCCC
NM_012099.1         GGATCCACCAAGAAGAGGAAGAAGCAGAGTCAGGAAAGCCGGATGCCAGAGACAGTGCCC
                    ************************************************************

HsCD00329684        CAAGAGGAGATGCCAGGGCCGCCACTGAATTCAGAGTCTGGGGAGGAGGCTCCCACAGGC
XM_005258425.1      CAAGAGGAGATGCCAGGGCCGCCACTGAATTCAGAGTCTGGGGAGGAGGCTCCCACAGGC
NM_012099.1         CAAGAGGAGATGCCAGGGCCGCCACTGAATTCAGAGTCTGGGGAGGAGGCTCCCACAGGC
                    ************************************************************

HsCD00329684        CGGGACAAGAAGCGGAAGAAGCAGCAGCAGCAGCCTGTGTTG
XM_005258425.1      CGGGACAAGAAGCGGAAGCAGCAGCAGCAGCAGCCTGTGTAG
NM_012099.1         CGGGACAAGAAGCGGAAGCAGCAGCAGCAGCAGCCTGTGTAG
                    ******************.*********************:*

Protein

UniProt : O15446
HPRD : 00125

Clone

Cloning Information : 268141
HIP Master Clone ID : 193183
Original Clone ID : FLH268141.01L

Organism


TAX_ID : 9606
Species Specific ID: 10849

Genome

Chromosome : 19
Map Location : 19q13.3
Ensembl : ENSG00000117877

Reagents

Labome : CD3EAP-antibody