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ZNF582 (Homo sapiens) in pLenti6.2/V5-DEST (V5-tagged lentiviral mammalian expression vector)

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Explanation of Terms


Gene: Gene Symbol:  ZNF582
Gene Name:  zinc finger protein 582
Sequence             
Original Clone ID: FLH268145.01L
Keyword: None
Species: Homo sapiens
Type: cDNA
Vector Name: pLenti6.2/V5-DEST               Format:  FUSION
Source: HIP
Description: None
Comments: None
Mutation/
Discrepancy :
No / No
Publications: None
Authors:
HsCD00329688

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AVAILABLE
No restriction
Special MTA: None

 

Insert sequence: 1554nts         Open reading frame : 1 to 1554
Reference Sequence Alignment

ATGTCCCTTGGGTCAGAATTGTTCAGGGATGTGGCCATAGTCTTCTCCCAAGAAGAATGG
CAGTGGTTGGCACCTGCTCAGAGGGATTTGTACAGAGACGTGATGTTGGAGACCTACAGC
AACCTGGTCTCACTGGGTCTTGCCGTTTCCAAACCTGATGTGATCTCCTTCCTAGAGCAA
GGCAAAGAGCCCTGGATGGTGGAGAGAGTGGTGTCTGGAGGCCTGTGTCCAGTATTGGAG
TCCAGATATGATACCAAGGAATTATTTCCAAAGCAGCATGTTTATGAAGTCGAATCACCC
CAATGGGAGATAATGGAAAGCCTTACAAGTTATGGTCTTGAGTGTTCAAGTTTCCAAGAT
GATTGGGAATGCAGAAACCAGTTTGACAGACAACAGGGAAATCCAGACAGACATTTCCAT
CAGATGATCATCAGACATGAAGAAATGCCCACTTTTGACCAGCATGCATCCCTTACTTTT
TATCAGAAAATTCATACTAGAGAAAAACCTTTTGGGTATAATAAATGTAGAAAAGACTTC
TGGCAAAAGGAACTCCTTATTAATCATCAAGGAATTTATACTAATGAGAAACCCTATAAA
TGTAAGGAATGTGGGAAGGCCTTTAAATATGGCTCACGACTAATTCAACATGAGAATATT
CATTCTGGTAAGAAACCATATGAATGTAAGGAATGTGGAAAGGCCTTCAATTCTGGTTCA
AATTTTATACAACATCAGAGAGTTCATACTGGTGAGAAACCGTATGAATGTAAAGATTGT
GAGAAGGCCTTTAGTCGAAGCTCACAGTTGATTGAACATCAGCGAACTCACACAGGCGAG
AAACCCTATCAGTGTAAGGAATGTGGCAAGGCCTTCAATCGGATCTCACATCTTAAAGTA
CATTATAGAATTCATACTGGTGAAAAACCCTATGCATGCAAGGAATGTGGGAAAACCTTT
AGTCATCGTTCTCAGTTGATTCAACATCAGACTGTTCATACTGGCAGGAAACTCTATGAA
TGTAAGGAATGTGGGAAGGCTTTTAATCAAGGCTCAACTCTTATACGACATCAGAGAATT
CATACCGGTGAGAAACCCTATGAGTGCAAAGTATGTGGAAAGGCCTTTAGAGTGAGCTCA
CAACTCAAGCAACATCAGAGAATTCACACTGGAGAGAAACCCTACCAATGTAAGGTATGT
GGTAGGGCCTTCAAACGGGTCTCACATCTTACTGTACATTACAGAATTCATACAGGTGAA
AAGCCATATGAATGTAAGGAATGTGGGAAGGCTTTTAGTCATTGCTCACAATTGATTCAT
CATCAGGTTATTCACACTGAGAAAAAGCCCTATGAATATAAGGAATGTGAGAAGACCTTG
AGTCATGATTCAACTACCGTTCAACCTCAGAGAATGCATAATAGAGAAACACATGTGAAT
ATAATAAATGTAGAAAAGCCTTCCATCAGCAGTTACCCCTTACTAATCATCAGAGAATTT
ATGTTGGCAAGCAACCATATGAATGGAAGTAATGGGGAAAGCCCATTAGCCTTG


Coding Sequence Details
Insert Sequence Verified?: N Verification Method: Insert was fully sequenced in parent vector
Reference Sequence Annotations:
End on reference sequence 1635
Start on reference sequence 82
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
5p GTACAAAAAAGCAGGCTCCACC(atg-goi)

3' Linker Sequence:
5p (goi-stop)GACCCAGCTTTCTTGTAC

 

 Recommended Growth Condition:

Distributed in bacterial strain : DH5-alpha T1 phage resistant
Antibiotic Selection: Host Type:  bacterial    Marker:  ampicillin
Host Type:  mammalian    Marker:  blasticidin
Bacterial Selection Condition:  100 ug/mL ampicillin
Growth Condition:  Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:  Commonly used conditions for ampicillin resistant plasmid clones.
Protein Expression Results: None
Recommended expression in: Not Applicable
       DyNA Vector Map

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Vector Name: pLenti6.2/V5-DEST

pLenti6.2V5-DEST in advanced viewed

Synonyms: pLenti6.2/V5-DEST
Sequencing Primer: Forward:  CMV forward
Reverse:  V5 reverse
Description: Lentiviral mammalian expression vector;CMV promoter and C-terminal V5 tag; ampicillin resistance in bacteria; blasticidin resistance in mammalian cells; Gateway recombinational cloning.
Comments: None
Size (bp): 8813
Parent Vector: None
Empty Vector: None
Properties: Gateway, acceptor (destination), lentiviral infection, mammalian expression, recombinational cloning, with tag/fusion/marker
Author Name: Invitrogen
Publications: None
Vector Map:         Vector Sequence:
Sequence:

 



Vector Features:

Type Name Description Start Position End Position
bacterial origin pUC ori pUC origin of replication 7733 8406
death cassette ccdB ccdB death cassette (removed when insert is present) 3767 3976
packaging signal HIV-1 psi HIV-1 psi packaging signal 521 565
poly-A signal SV40 SV40 polyadenylation signal 5639 5770
promoter RSV RSV/5' LTR hybrid promoter 1 410
promoter CMV CMV promoter driving gene of interest 1809 2392
promoter PGK PGK promoter to drive selection marker in mammalian cells 4266 4771
recombination site attR2 attR2 recombinational cloning site 4127 4142
recombination site attR1 attR1 recombinational cloning site 2440 2456
response element HIV-1 RRE HIV-1 Rev response element (RRE) 1075 1308
selectable marker AmpR Ampicillin resistance gene for selection in bacteria 6728 7588
selectable marker BlastR Blasticidin resistance gene in mammalian cells 4849 5249
selectable marker CmR chloramphenicol resistance marker (removed in plasmids with insert) 2673 3329
splice acceptor site 3' splice acceptor two 3' splice acceptors at 1656 and 1684 1656 1656
splice donor site 5' splice donor 5' splice donor 520 520
tag V5 C-terminal V5 epitope tag 4197 4238

 

  • Gene
  • Pathways
  • Protein
  • Clone
  • Organism
  • Genome
  • Reagents

Gene

 

Gene Symbol : ZNF582
Symbol Nomenclature : ZNF582
Full Nomenclature : zinc finger protein 582
GENEID : 147948
HGNC : 26421
MIM : 615600
Vega : OTTHUMG00000181939
Target GenBank: BC101009

 

Reference Sequence Alignment

  

HsCD00329688      ATGTCCCTTGGGTCAGAATTGTTCAGGGATGTGGCCATAGTCTTCTCCCAAGAAGAATGG
NM_144690.1       ATGTCCCTTGGGTCAGAATTGTTCAGGGATGTGGCCATAGTCTTCTCCCAAGAAGAATGG
                  ************************************************************

HsCD00329688      CAGTGGTTGGCACCTGCTCAGAGGGATTTGTACAGAGACGTGATGTTGGAGACCTACAGC
NM_144690.1       CAGTGGTTGGCACCTGCTCAGAGGGATTTGTACAGAGACGTGATGTTGGAGACCTACAGC
                  ************************************************************

HsCD00329688      AACCTGGTCTCACTGGGTCTTGCCGTTTCCAAACCTGATGTGATCTCCTTCCTAGAGCAA
NM_144690.1       AACCTGGTCTCACTGGGTCTTGCCGTTTCCAAACCTGATGTGATCTCCTTCCTAGAGCAA
                  ************************************************************

HsCD00329688      GGCAAAGAGCCCTGGATGGTGGAGAGAGTGGTGTCTGGAGGCCTGTGTCCAGTATTGGAG
NM_144690.1       GGCAAAGAGCCCTGGATGGTGGAGAGAGTGGTGTCTGGAGGCCTGTGTCCAGTATTGGAG
                  ************************************************************

HsCD00329688      TCCAGATATGATACCAAGGAATTATTTCCAAAGCAGCATGTTTATGAAGTCGAATCACCC
NM_144690.1       TCCAGATATGATACCAAGGAATTATTTCCAAAGCAGCATGTTTATGAAGTCGAATCACCC
                  ************************************************************

HsCD00329688      CAATGGGAGATAATGGAAAGCCTTACAAGTTATGGTCTTGAGTGTTCAAGTTTCCAAGAT
NM_144690.1       CAATGGGAGATAATGGAAAGCCTTACAAGTTATGGTCTTGAGTGTTCAAGTTTCCAAGAT
                  ************************************************************

HsCD00329688      GATTGGGAATGCAGAAACCAGTTTGACAGACAACAGGGAAATCCAGACAGACATTTCCAT
NM_144690.1       GATTGGGAATGCAGAAACCAGTTTGACAGACAACAGGGAAATCCAGACAGACATTTCCAT
                  ************************************************************

HsCD00329688      CAGATGATCATCAGACATGAAGAAATGCCCACTTTTGACCAGCATGCATCCCTTACTTTT
NM_144690.1       CAGATGATCATCAGACATGAAGAAATGCCCACTTTTGACCAGCATGCATCCCTTACTTTT
                  ************************************************************

HsCD00329688      TATCAGAAAATTCATACTAGAGAAAAACCTTTTGGGTATAATAAATGTAGAAAAGACTTC
NM_144690.1       TATCAGAAAATTCATACTAGAGAAAAACCTTTTGGGTATAATAAATGTAGAAAAGACTTC
                  ************************************************************

HsCD00329688      TGGCAAAAGGAACTCCTTATTAATCATCAAGGAATTTATACTAATGAGAAACCCTATAAA
NM_144690.1       TGGCAAAAGGAACTCCTTATTAATCATCAAGGAATTTATACTAATGAGAAACCCTATAAA
                  ************************************************************

HsCD00329688      TGTAAGGAATGTGGGAAGGCCTTTAAATATGGCTCACGACTAATTCAACATGAGAATATT
NM_144690.1       TGTAAGGAATGTGGGAAGGCCTTTAAATATGGCTCACGACTAATTCAACATGAGAATATT
                  ************************************************************

HsCD00329688      CATTCTGGTAAGAAACCATATGAATGTAAGGAATGTGGAAAGGCCTTCAATTCTGGTTCA
NM_144690.1       CATTCTGGTAAGAAACCATATGAATGTAAGGAATGTGGAAAGGCCTTCAATTCTGGTTCA
                  ************************************************************

HsCD00329688      AATTTTATACAACATCAGAGAGTTCATACTGGTGAGAAACCGTATGAATGTAAAGATTGT
NM_144690.1       AATTTTATACAACATCAGAGAGTTCATACTGGTGAGAAACCGTATGAATGTAAAGATTGT
                  ************************************************************

HsCD00329688      GAGAAGGCCTTTAGTCGAAGCTCACAGTTGATTGAACATCAGCGAACTCACACAGGCGAG
NM_144690.1       GAGAAGGCCTTTAGTCGAAGCTCACAGTTGATTGAACATCAGCGAACTCACACAGGCGAG
                  ************************************************************

HsCD00329688      AAACCCTATCAGTGTAAGGAATGTGGCAAGGCCTTCAATCGGATCTCACATCTTAAAGTA
NM_144690.1       AAACCCTATCAGTGTAAGGAATGTGGCAAGGCCTTCAATCGGATCTCACATCTTAAAGTA
                  ************************************************************

HsCD00329688      CATTATAGAATTCATACTGGTGAAAAACCCTATGCATGCAAGGAATGTGGGAAAACCTTT
NM_144690.1       CATTATAGAATTCATACTGGTGAAAAACCCTATGCATGCAAGGAATGTGGGAAAACCTTT
                  ************************************************************

HsCD00329688      AGTCATCGTTCTCAGTTGATTCAACATCAGACTGTTCATACTGGCAGGAAACTCTATGAA
NM_144690.1       AGTCATCGTTCTCAGTTGATTCAACATCAGACTGTTCATACTGGCAGGAAACTCTATGAA
                  ************************************************************

HsCD00329688      TGTAAGGAATGTGGGAAGGCTTTTAATCAAGGCTCAACTCTTATACGACATCAGAGAATT
NM_144690.1       TGTAAGGAATGTGGGAAGGCTTTTAATCAAGGCTCAACTCTTATACGACATCAGAGAATT
                  ************************************************************

HsCD00329688      CATACCGGTGAGAAACCCTATGAGTGCAAAGTATGTGGAAAGGCCTTTAGAGTGAGCTCA
NM_144690.1       CATACCGGTGAGAAACCCTATGAGTGCAAAGTATGTGGAAAGGCCTTTAGAGTGAGCTCA
                  ************************************************************

HsCD00329688      CAACTCAAGCAACATCAGAGAATTCACACTGGAGAGAAACCCTACCAATGTAAGGTATGT
NM_144690.1       CAACTCAAGCAACATCAGAGAATTCACACTGGAGAGAAACCCTACCAATGTAAGGTATGT
                  ************************************************************

HsCD00329688      GGTAGGGCCTTCAAACGGGTCTCACATCTTACTGTACATTACAGAATTCATACAGGTGAA
NM_144690.1       GGTAGGGCCTTCAAACGGGTCTCACATCTTACTGTACATTACAGAATTCATACAGGTGAA
                  ************************************************************

HsCD00329688      AAGCCATATGAATGTAAGGAATGTGGGAAGGCTTTTAGTCATTGCTCACAATTGATTCAT
NM_144690.1       AAGCCATATGAATGTAAGGAATGTGGGAAGGCTTTTAGTCATTGCTCACAATTGATTCAT
                  ************************************************************

HsCD00329688      CATCAGGTTATTCACACTGAGAAAAAGCCCTATGAATATAAGGAATGTGAGAAGACCTTG
NM_144690.1       CATCAGGTTATTCACACTGAGAAAAAGCCCTATGAATATAAGGAATGTGAGAAGACCTTG
                  ************************************************************

HsCD00329688      AGTCATGATTCAACTACCGTTCAACCTCAGAGAATGCATAATAGAGAAACACATGTGAAT
NM_144690.1       AGTCATGATTCAACTACCGTTCAACCTCAGAGAATGCATAATAGAGAAACACATGTGAAT
                  ************************************************************

HsCD00329688      ATAATAAATGTAGAAAAGCCTTCCATCAGCAGTTACCCCTTACTAATCATCAGAGAATTT
NM_144690.1       ATAATAAATGTAGAAAAGCCTTCCATCAGCAGTTACCCCTTACTAATCATCAGAGAATTT
                  ************************************************************

HsCD00329688      ATGTTGGCAAGCAACCATATGAATGGAAGTAATGGGGAAAGCCCATTAGCCTTG
NM_144690.1       ATGTTGGCAAGCAACCATATGAATGGAAGTAATGGGGAAAGCCCATTAGCCTAG
                  ****************************************************:*

Pathways

Reactome : Gene Expression
Reactome : Generic Transcription Pathway

Protein

SMART domain : KRAB : krueppel associated box
SMART domain : ZnF_C2H2 : zinc finger
UniProt : Q96NG8
HPRD : 15859

Clone

Cloning Information : 268145
HIP Master Clone ID : 193187
Original Clone ID : FLH268145.01L

Organism


TAX_ID : 9606
Species Specific ID: 147948

Genome

Chromosome : 19
Map Location : 19q13.43
Ensembl : ENSG00000018869

Reagents

Labome : ZNF582-antibody