DNASU Plasmid Repository • 480.965.5697 | Email

KIAA0961 (H. sapiens ) in pLenti6.2/V5-DEST

TOP

Explanation of Terms


Gene: Gene Symbol:  ZFP30
Gene Name:  ZFP30 zinc finger protein
Sequence             
Original Clone ID: FLH268147.01L
Keyword: None
Species: Homo sapiens
Type: cDNA
Vector Name: pLenti6.2/V5-DEST               Format:  FUSION
Source: HIP
Description: None
Comments: None
Mutation/
Discrepancy :
No / No
Publications: None
Authors:
HsCD00329690

Price:  Login for Pricing
AVAILABLE
No restriction
Special MTA: None

 

Insert sequence: 1560nts         Open reading frame : 1 to 1560
Reference Sequence Alignment

ATGGCTCGTGATTTGGTGATGTTCAGAGATGTGGCTGTAGACTTTTCTCAGGAAGAATGG
GAATGCCTGAACTCATATCAGAGGAATTTGTACAGAGATGTGATATTAGAGAACTATAGC
AACTTGGTGTCACTGGCAGGATGTTCTATTTCTAAGCCAGATGTGATCACCCTATTGGAA
CAAGGGAAAGAGCCCTGGATGGTTGTGAGGGATGAGAAAAGAAGATGGACTCTAGATTTG
GAATCCAGATATGACACTAAAAAGTTATTTCAAGGAAAGGATATTTATGAAATGAACTTA
TCTCAGTGGAAGGTAATGGAAAGAATTAAAAGCTGTGGACTTGAAGAACAAGAAAGTCCT
CATGAGGTGTGTTTCAGGCAAGTGACAAAAACCACCTCTGAAAAAATGCCTACTTACAGA
AAACTCACATCTCTTCCTCTGTATCAGAAAAGTCATAACAGAGAGAAACCCTACGAATGT
GGGGAATGTGGGAAGGCTTTTAGAGTACGACAACAACTTACTTTCCATCAAAGAATTCAT
ACTGGTGAGAAACCCTATGAATGTAAAGAATGTGGAAAAGCCTTTAGACAGTGTGCCCAC
CTCAGTCGACATCAGAGAATTCATACTTCTGACAAACTCTATGAATGTAAAAAATGTGGA
AAGATCTTCACATGTGGCTCAGACCTTCGAGTACATCAGAGAATTCATATTGGTGAGAAG
CCGTATGAATGTAAAGAATGTGGGAAAGCCTTTAGAGTTAGAGGACAACTTAATCTCCAT
CAAAGGATTCACACGGGTGAGAAACCCTATGAATGTAAAGAATGTGGGAAGGCCTTTAGG
CAGTATGCACACCTTACTCGACATCAGAGACTGAACATTGCTGAGAAGTGCTATGAGTGT
AAGGAATGTGGTCAGGCCTTTCTGTGTAGTACAGGCCTTCGACTACATCACAAACTTCAT
ACTGGAGAAAAACCCTATGAATGTAAGGAGTGTGGAAAGGCCTTTAGAGTGCGGCAGCAA
CTTACTCTCCATCAGAGAATTCACACTGGTGAGAAGCCTTATGATTGTAAGGAATGTGGG
AAGACTTTCAGTCGTGGCTATCATCTAACTCTCCATCAGAGAATACATACTGGTGAAAAG
CCCTATGAATGTAAGGAATGTCAGAAGTTCTTTCGTCGTTACTCAGAACTTATTTCACAT
CAGGGTATTCACATTGGAGAGAAACCTTATGAATGTAAGGAATGTGGGAAGGCATTTAGG
CTGTTCTCACAGCTTACTCAACATCAGAGTATTCATTTTGGTGAGAAACCCTTTAAGTGT
AAGGAATGTGAGAAGACTTTTAGACTGCTTTCACAACTTACCCAACATCAAAGTATTCAT
ACTGGTGAAAAGCCCTATGACTGTAAGGAATGTGGAAAGGCCTTTAGACTTCATTCATCA
CTGATTCAACATCAGAGAATTCATTCTGGTGAGAAACCATACAAATGTAAGGAATGTAAA
AAGGCATTTAGACAACATTCACATCTTACTTACCATCAGCGAATTCATAATGTAACTTTG


Coding Sequence Details
Insert Sequence Verified?: N Verification Method: Insert was fully sequenced in parent vector
Reference Sequence Annotations:
End on reference sequence 2147
Start on reference sequence 588
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
5p GTACAAAAAAGCAGGCTCCACC(atg-goi)

3' Linker Sequence:
5p (goi-stop)GACCCAGCTTTCTTGTAC

 

 Recommended Growth Condition:

Distributed in bacterial strain : DH5-alpha T1 phage resistant
Antibiotic Selection: Host Type:  bacterial    Marker:  ampicillin
Host Type:  mammalian    Marker:  blasticidin
Bacterial Selection Condition:  100 ug/mL ampicillin
Growth Condition:  Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:  Commonly used conditions for ampicillin resistant plasmid clones.
Protein Expression Results: None
Recommended expression in: Not Applicable
       DyNA Vector Map

         Powered by LabGenius

Vector Name: pLenti6.2/V5-DEST

pLenti6.2V5-DEST in advanced viewed

Synonyms: pLenti6.2/V5-DEST
Sequencing Primer: Forward:  CMV forward
Reverse:  V5 reverse
Description: Lentiviral mammalian expression vector;CMV promoter and C-terminal V5 tag; ampicillin resistance in bacteria; blasticidin resistance in mammalian cells; Gateway recombinational cloning.
Comments: None
Size (bp): 8813
Parent Vector: None
Empty Vector: None
Properties: Gateway, acceptor (destination), lentiviral infection, mammalian expression, recombinational cloning, with tag/fusion/marker
Author Name: Invitrogen
Publications: None
Vector Map:         Vector Sequence:
Sequence:

 



Vector Features:

Type Name Description Start Position End Position
bacterial origin pUC ori pUC origin of replication 7733 8406
death cassette ccdB ccdB death cassette (removed when insert is present) 3767 3976
packaging signal HIV-1 psi HIV-1 psi packaging signal 521 565
poly-A signal SV40 SV40 polyadenylation signal 5639 5770
promoter RSV RSV/5' LTR hybrid promoter 1 410
promoter CMV CMV promoter driving gene of interest 1809 2392
promoter PGK PGK promoter to drive selection marker in mammalian cells 4266 4771
recombination site attR2 attR2 recombinational cloning site 4127 4142
recombination site attR1 attR1 recombinational cloning site 2440 2456
response element HIV-1 RRE HIV-1 Rev response element (RRE) 1075 1308
selectable marker AmpR Ampicillin resistance gene for selection in bacteria 6728 7588
selectable marker BlastR Blasticidin resistance gene in mammalian cells 4849 5249
selectable marker CmR chloramphenicol resistance marker (removed in plasmids with insert) 2673 3329
splice acceptor site 3' splice acceptor two 3' splice acceptors at 1656 and 1684 1656 1656
splice donor site 5' splice donor 5' splice donor 520 520
tag V5 C-terminal V5 epitope tag 4197 4238

 

  • Gene
  • Pathways
  • Protein
  • Clone
  • Organism
  • Genome
  • Reagents

Gene

 

Gene Symbol : ZFP30
Symbol Nomenclature : ZFP30
SYNONYM : ZNF745
Designation : zfp-30|zinc finger protein 30 homolog|zinc finger protein 745
Full Nomenclature : ZFP30 zinc finger protein
GENEID : 22835
HGNC : 29555
Vega : OTTHUMG00000048177
Target GenBank: BC041087

 

Reference Sequence Alignment

  

HsCD00329690      ATGGCTCGTGATTTGGTGATGTTCAGAGATGTGGCTGTAGACTTTTCTCAGGAAGAATGG
NM_014898.2       ATGGCTCGTGATTTGGTGATGTTCAGAGATGTGGCTGTAGACTTTTCTCAGGAAGAATGG
                  ************************************************************

HsCD00329690      GAATGCCTGAACTCATATCAGAGGAATTTGTACAGAGATGTGATATTAGAGAACTATAGC
NM_014898.2       GAATGCCTGAACTCATATCAGAGGAATTTGTACAGAGATGTGATATTAGAGAACTATAGC
                  ************************************************************

HsCD00329690      AACTTGGTGTCACTGGCAGGATGTTCTATTTCTAAGCCAGATGTGATCACCCTATTGGAA
NM_014898.2       AACTTGGTGTCACTGGCAGGATGTTCTATTTCTAAGCCAGATGTGATCACCCTATTGGAA
                  ************************************************************

HsCD00329690      CAAGGGAAAGAGCCCTGGATGGTTGTGAGGGATGAGAAAAGAAGATGGACTCTAGATTTG
NM_014898.2       CAAGGGAAAGAGCCCTGGATGGTTGTGAGGGATGAGAAAAGAAGATGGACTCTAGATTTG
                  ************************************************************

HsCD00329690      GAATCCAGATATGACACTAAAAAGTTATTTCAAGGAAAGGATATTTATGAAATGAACTTA
NM_014898.2       GAATCCAGATATGACACTAAAAAGTTATTTCAAGGAAAGGATATTTATGAAATGAACTTA
                  ************************************************************

HsCD00329690      TCTCAGTGGAAGGTAATGGAAAGAATTAAAAGCTGTGGACTTGAAGAACAAGAAAGTCCT
NM_014898.2       TCTCAGTGGAAGGTAATGGAAAGAATTAAAAGCTGTGGACTTGAAGAACAAGAAAGTCCT
                  ************************************************************

HsCD00329690      CATGAGGTGTGTTTCAGGCAAGTGACAAAAACCACCTCTGAAAAAATGCCTACTTACAGA
NM_014898.2       CATGAGGTGTGTTTCAGGCAAGTGACAAAAACCACCTCTGAAAAAATGCCTACTTACAGA
                  ************************************************************

HsCD00329690      AAACTCACATCTCTTCCTCTGTATCAGAAAAGTCATAACAGAGAGAAACCCTACGAATGT
NM_014898.2       AAACTCACATCTCTTCCTCTGTATCAGAAAAGTCATAACAGAGAGAAACCCTACGAATGT
                  ************************************************************

HsCD00329690      GGGGAATGTGGGAAGGCTTTTAGAGTACGACAACAACTTACTTTCCATCAAAGAATTCAT
NM_014898.2       GGGGAATGTGGGAAGGCTTTTAGAGTACGACAACAACTTACTTTCCATCAAAGAATTCAT
                  ************************************************************

HsCD00329690      ACTGGTGAGAAACCCTATGAATGTAAAGAATGTGGAAAAGCCTTTAGACAGTGTGCCCAC
NM_014898.2       ACTGGTGAGAAACCCTATGAATGTAAAGAATGTGGAAAAGCCTTTAGACAGTGTGCCCAC
                  ************************************************************

HsCD00329690      CTCAGTCGACATCAGAGAATTCATACTTCTGACAAACTCTATGAATGTAAAAAATGTGGA
NM_014898.2       CTCAGTCGACATCAGAGAATTCATACTTCTGACAAACTCTATGAATGTAAAAAATGTGGA
                  ************************************************************

HsCD00329690      AAGATCTTCACATGTGGCTCAGACCTTCGAGTACATCAGAGAATTCATATTGGTGAGAAG
NM_014898.2       AAGATCTTCACATGTGGCTCAGACCTTCGAGTACATCAGAGAATTCATATTGGTGAGAAG
                  ************************************************************

HsCD00329690      CCGTATGAATGTAAAGAATGTGGGAAAGCCTTTAGAGTTAGAGGACAACTTAATCTCCAT
NM_014898.2       CCGTATGAATGTAAAGAATGTGGGAAAGCCTTTAGAGTTAGAGGACAACTTAATCTCCAT
                  ************************************************************

HsCD00329690      CAAAGGATTCACACGGGTGAGAAACCCTATGAATGTAAAGAATGTGGGAAGGCCTTTAGG
NM_014898.2       CAAAGGATTCACACGGGTGAGAAACCCTATGAATGTAAAGAATGTGGGAAGGCCTTTAGG
                  ************************************************************

HsCD00329690      CAGTATGCACACCTTACTCGACATCAGAGACTGAACATTGCTGAGAAGTGCTATGAGTGT
NM_014898.2       CAGTATGCACACCTTACTCGACATCAGAGACTGAACATTGCTGAGAAGTGCTATGAGTGT
                  ************************************************************

HsCD00329690      AAGGAATGTGGTCAGGCCTTTCTGTGTAGTACAGGCCTTCGACTACATCACAAACTTCAT
NM_014898.2       AAGGAATGTGGTCAGGCCTTTCTGTGTAGTACAGGCCTTCGACTACATCACAAACTTCAT
                  ************************************************************

HsCD00329690      ACTGGAGAAAAACCCTATGAATGTAAGGAGTGTGGAAAGGCCTTTAGAGTGCGGCAGCAA
NM_014898.2       ACTGGAGAAAAACCCTATGAATGTAAGGAGTGTGGAAAGGCCTTTAGAGTGCGGCAGCAA
                  ************************************************************

HsCD00329690      CTTACTCTCCATCAGAGAATTCACACTGGTGAGAAGCCTTATGATTGTAAGGAATGTGGG
NM_014898.2       CTTACTCTCCATCAGAGAATTCACACTGGTGAGAAGCCTTATGATTGTAAGGAATGTGGG
                  ************************************************************

HsCD00329690      AAGACTTTCAGTCGTGGCTATCATCTAACTCTCCATCAGAGAATACATACTGGTGAAAAG
NM_014898.2       AAGACTTTCAGTCGTGGCTATCATCTAACTCTCCATCAGAGAATACATACTGGTGAAAAG
                  ************************************************************

HsCD00329690      CCCTATGAATGTAAGGAATGTCAGAAGTTCTTTCGTCGTTACTCAGAACTTATTTCACAT
NM_014898.2       CCCTATGAATGTAAGGAATGTCAGAAGTTCTTTCGTCGTTACTCAGAACTTATTTCACAT
                  ************************************************************

HsCD00329690      CAGGGTATTCACATTGGAGAGAAACCTTATGAATGTAAGGAATGTGGGAAGGCATTTAGG
NM_014898.2       CAGGGTATTCACATTGGAGAGAAACCTTATGAATGTAAGGAATGTGGGAAGGCATTTAGG
                  ************************************************************

HsCD00329690      CTGTTCTCACAGCTTACTCAACATCAGAGTATTCATTTTGGTGAGAAACCCTTTAAGTGT
NM_014898.2       CTGTTCTCACAGCTTACTCAACATCAGAGTATTCATTTTGGTGAGAAACCCTTTAAGTGT
                  ************************************************************

HsCD00329690      AAGGAATGTGAGAAGACTTTTAGACTGCTTTCACAACTTACCCAACATCAAAGTATTCAT
NM_014898.2       AAGGAATGTGAGAAGACTTTTAGACTGCTTTCACAACTTACCCAACATCAAAGTATTCAT
                  ************************************************************

HsCD00329690      ACTGGTGAAAAGCCCTATGACTGTAAGGAATGTGGAAAGGCCTTTAGACTTCATTCATCA
NM_014898.2       ACTGGTGAAAAGCCCTATGACTGTAAGGAATGTGGAAAGGCCTTTAGACTTCATTCATCA
                  ************************************************************

HsCD00329690      CTGATTCAACATCAGAGAATTCATTCTGGTGAGAAACCATACAAATGTAAGGAATGTAAA
NM_014898.2       CTGATTCAACATCAGAGAATTCATTCTGGTGAGAAACCATACAAATGTAAGGAATGTAAA
                  ************************************************************

HsCD00329690      AAGGCATTTAGACAACATTCACATCTTACTTACCATCAGCGAATTCATAATGTAACTTTG
NM_014898.2       AAGGCATTTAGACAACATTCACATCTTACTTACCATCAGCGAATTCATAATGTAACTTAA
                  **********************************************************:.

Pathways

Reactome : Gene Expression
Reactome : Generic Transcription Pathway

Protein

SMART domain : KRAB : krueppel associated box
SMART domain : ZnF_C2H2 : zinc finger
UniProt : D3Y2A0
UniProt : Q9Y2G7
HPRD : 17201

Clone

Cloning Information : 268147
HIP Master Clone ID : 193189
Original Clone ID : FLH268147.01L

Organism


TAX_ID : 9606
Species Specific ID: 22835

Genome

Chromosome : 19
Map Location : 19q13.12
Ensembl : ENSG00000120784

Reagents

Labome : ZFP30-antibody