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HIST1H1B (H. sapiens ) in pLenti6.2/V5-DEST


Explanation of Terms

Gene: Gene Symbol:  HIST1H1B
Gene Name:  histone cluster 1, H1b
Original Clone ID: FLH268148.01L
Keyword: None
Species: Homo sapiens
Type: cDNA
Vector Name: pLenti6.2/V5-DEST               Format:  FUSION
Source: HIP
Description: None
Comments: None
Discrepancy :
No / No
Publications: None

Price:  Login for Pricing
No restriction
Special MTA: None


Insert sequence: 681nts        
Open reading frame : 1 to 681


Coding Sequence Details
Insert Sequence Verified?: N Verification Method: Insert was fully sequenced in parent vector
Reference Sequence Annotations:
Start on reference sequence 26
End on reference sequence 706
5' Linker Sequence: None
3' Linker Sequence: None


 Recommended Growth Condition:

Distributed in bacterial strain : DH5-alpha T1 phage resistant
Antibiotic Selection: Host Type:  bacterial    Marker:  ampicillin
Host Type:  mammalian    Marker:  blasticidin
Bacterial Selection Condition:  100 ug/mL ampicillin
Growth Condition:  Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:  Commonly used conditions for ampicillin resistant plasmid clones.
Protein Expression Results: None
Recommended expression in: Not Applicable
       DyNA Vector Map

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Vector Name: pLenti6.2/V5-DEST

pLenti6.2V5-DEST in advanced viewed

Synonyms: pLenti6.2/V5-DEST
Sequencing Primer: Forward:  CMV forward
Reverse:  V5 reverse
Description: with tag/fusion/marker assay, Gateway®, acceptor (destination), recombinational cloning clone, lentiviral infection, mammalian expression expression with CMV and PGK and BlastR and AmpR and RSV and 5' splice donor and HIV-1 psi and HIV-1 RRE and 3' splice acceptor and V5 and SV40 and pUC ori and attR1 and attR2 and ccdB and CmR; blasticidin resistance in mammalian, ampicillin resistance in bacterial;
Comments: None
Size (bp): 8813
Parent Vector: None
Empty Vector: None
Properties: Gateway, acceptor (destination), lentiviral infection, mammalian expression, recombinational cloning, with tag/fusion/marker
Author Name: Invitrogen
Publications: None
Vector Map:         Vector Sequence:


Vector Features:

Type Name Description Start Position End Position
bacterial origin pUC ori pUC origin of replication 7733 8406
death cassette ccdB ccdB death cassette (removed when insert is present) 3767 3976
packaging signal HIV-1 psi HIV-1 psi packaging signal 521 565
poly-A signal SV40 SV40 polyadenylation signal 5639 5770
promoter CMV CMV promoter driving gene of interest 1809 2392
promoter PGK PGK promoter to drive selection marker in mammalian cells 4266 4771
promoter RSV RSV/5' LTR hybrid promoter 1 410
recombination site attR1 attR1 recombinational cloning site 2440 2456
recombination site attR2 attR2 recombinational cloning site 4127 4142
response element HIV-1 RRE HIV-1 Rev response element (RRE) 1075 1308
selectable marker BlastR Blasticidin resistance gene in mammalian cells 4849 5249
selectable marker CmR chloramphenicol resistance marker (removed in plasmids with insert) 2673 3329
selectable marker AmpR Ampicillin resistance gene for selection in bacteria 6728 7588
splice acceptor site 3' splice acceptor two 3' splice acceptors at 1656 and 1684 1656 1656
splice donor site 5' splice donor 5' splice donor 520 520
tag V5 C-terminal V5 epitope tag 4197 4238


  • Gene
  • Pathways
  • Protein
  • Clone
  • Organism
  • Genome
  • Reagents



Gene Symbol : HIST1H1B
Symbol Nomenclature : HIST1H1B
SYNONYM : H1; H1.5; H1B; H1F5; H1s-3
Designation : H1 histone family, member 5|histone 1, H1b|histone H1.5|histone H1a|histone H1b|histone H1s-3
Full Nomenclature : histone cluster 1, H1b
GENEID : 3009
HGNC : 4719
MIM : 142711
Vega : OTTHUMG00000016139
Target GenBank: BC101583



Reactome : Activation of DNA fragmentation factor
Reactome : Apoptosis
Reactome : Apoptosis induced DNA fragmentation
Reactome : Apoptotic execution phase
Reactome : Cellular responses to stress
Reactome : Cellular Senescence
Reactome : DNA Damage/Telomere Stress Induced Senescence
Reactome : Formation of Senescence-Associated Heterochromatin Foci (SAHF)


SMART domain : H15 : Domain in histone families 1 and 5
UniProt : P16401
HPRD : 00821


Cloning Information : 268148
HIP Master Clone ID : 193190
Original Clone ID : FLH268148.01L


TAX_ID : 9606
Species Specific ID: 3009


Chromosome : 6
Map Location : 6p22.1
Ensembl : ENSG00000184357


Labome : HIST1H1B-antibody