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TBC1D10B (H. sapiens ) in pLenti6.2/V5-DEST

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Explanation of Terms


Gene: Gene Symbol:  TBC1D10B
Gene Name:  TBC1 domain family, member 10B
Sequence             
Original Clone ID: FLH268149.01L
Keyword: None
Species: Homo sapiens
Type: cDNA
Vector Name: pLenti6.2/V5-DEST               Format:  FUSION
Source: HIP
Description: None
Comments: None
Mutation/
Discrepancy :
No / No
Publications: None
Authors:
HsCD00329692

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AVAILABLE
No restriction
Special MTA: None

 

Insert sequence: 1602nts         Open reading frame : 1 to 1602
Reference Sequence Alignment

ATGTCTGGGACCTTGGAGTCCTTGGCGGATGATGTGAGCTCCATGGGCTCAGATTCAGAG
ATAAACGGGCTGGCCCTGCGCAAGACGGACAAGTATGGCTTCCTTGGGGGCAGCCAGTAC
TCGGGCAGCCTAGAGAGCTCCATTCCCGTGGACGTGGCTCGGCAGCGGGAGCTCAAATGG
CTGGACATGTTCAGTAACTGGGATAAGTGGCTGTCACGGCGATTCCAGAAGGTGAAGCTG
CGCTGCCGGAAGGGGATCCCCTCCTCTCTCAGAGCCAAGGCCTGGCAGTACCTGTCTAAT
AGCAAGGAACTTCTGGAGCAGAACCCAGGAAAGTTTGAGGAGCTGGAACGGGCTCCTGGG
GACCCCAAGTGGCTGGATGTGATTGAGAAGGACCTGCACCGCCAGTTCCCTTTCCACGAG
ATGTTTGCTGCTCGAGGGGGGCATGGGCAACAGGACCTGTACCGAATCCTGAAGGCCTAC
ACCATCTACCGGCCTGACGAGGGTTACTGCCAGGCCCAGGCCCCCGTGGCTGCGGTCCTG
CTCATGCACATGCCTGCGGAGCAAGCCTTTTGGTGCCTGGTGCAGATCTGCGACAAGTAC
CTCCCAGGTTACTACAGTGCAGGGCTGGAGGCCATTCAGCTGGACGGGGAGATCTTTTTT
GCACTCCTGCGCCGGGCCTCCCCGCTGGCGCATCGCCACCTGCGGCGGCAGCGCATTGAC
CCTGTGCTCTACATGACGGAGTGGTTCATGTGCATCTTCGCCCGCACCCTGCCCTGGGCG
TCGGTGCTGCGTGTCTGGGACATGTTTTTCTGTGAAGGCGTTAAGATCATCTTCCGGGTG
GCCCTGGTCCTGCTGCGCCACACGCTGGGCTCAGTGGAGAAGCTGCGCTCCTGCCAAGGC
ATGTATGAGACCATGGAGCAGCTGCGTAACCTGCCCCAGCAGTGCATGCAGGAAGACTTC
CTGGTGCATGAGGTGACCAATCTGCCGGTGACAGAAGCACTGATTGAGCGGGAGAATGCA
GCCCAGCTCAAGAAGTGGCGGGAAACGCGGGGGGAGCTGCAGTATCGGCCCTCACGGCGA
CTGCATGGGTCCCGGGCCATCCACGAGGAGCGCCGGCGGCAACAGCCACCCCTGGGCCCC
TCCTCCAGCCTCCTCAGCCTCCCTGGCCTCAAGAGCCGAGGCTCCCGGGCAGCTGGAGGG
GCCCCGTCCCCGCCGCCCCCCGTCCGCAGAGCCAGTGCTGGGCCTGCCCCAGGGCCTGTG
GTCACTGCTGAGGGACTGCATCCATCCCTTCCCTCACCCACTGGCAATAGCACCCCCTTG
GGTTCCAGCAAGGAGACCCGGAAGCAGGAGAAGGAGCGGCAGAAACAGGAGAAGGAGCGG
CAGAAACAGGAGAAGGAGCGGGAGAAGGAGCGGCAGAAGCAGGAGAAAGAGCGAGAGAAG
CAGGAAAAGGAGCGAGAGAAGCAGGAGAAGGAGCGGCAGAAGCAGGAGAAGAAGGCTCAA
GGCCGGAAGCTTTCGCTGCGTCGAAAGGCAGATGGGCCCCCAGGCCCCCATGATGGTGGG
GACAGGCCCTCAGCCGAGGCCCGGCAGGACGCTTACTTCTTG


Coding Sequence Details
Insert Sequence Verified?: N Verification Method: Insert was fully sequenced in parent vector
Reference Sequence Annotations:
End on reference sequence 1673
Start on reference sequence 72
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
5p GTACAAAAAAGCAGGCTCCACC(atg-goi)

3' Linker Sequence:
5p (goi-stop)GACCCAGCTTTCTTGTAC

 

 Recommended Growth Condition:

Distributed in bacterial strain : DH5-alpha T1 phage resistant
Antibiotic Selection: Host Type:  bacterial    Marker:  ampicillin
Host Type:  mammalian    Marker:  blasticidin
Bacterial Selection Condition:  100 ug/mL ampicillin
Growth Condition:  Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:  Commonly used conditions for ampicillin resistant plasmid clones.
Protein Expression Results: None
Recommended expression in: Not Applicable
       DyNA Vector Map

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Vector Name: pLenti6.2/V5-DEST

pLenti6.2V5-DEST in advanced viewed

Synonyms: pLenti6.2/V5-DEST
Sequencing Primer: Forward:  CMV forward
Reverse:  V5 reverse
Description: Lentiviral mammalian expression vector;CMV promoter and C-terminal V5 tag; ampicillin resistance in bacteria; blasticidin resistance in mammalian cells; Gateway recombinational cloning.
Comments: None
Size (bp): 8813
Parent Vector: None
Empty Vector: None
Properties: Gateway, acceptor (destination), lentiviral infection, mammalian expression, recombinational cloning, with tag/fusion/marker
Author Name: Invitrogen
Publications: None
Vector Map:         Vector Sequence:
Sequence:

 



Vector Features:

Type Name Description Start Position End Position
bacterial origin pUC ori pUC origin of replication 7733 8406
death cassette ccdB ccdB death cassette (removed when insert is present) 3767 3976
packaging signal HIV-1 psi HIV-1 psi packaging signal 521 565
poly-A signal SV40 SV40 polyadenylation signal 5639 5770
promoter RSV RSV/5' LTR hybrid promoter 1 410
promoter CMV CMV promoter driving gene of interest 1809 2392
promoter PGK PGK promoter to drive selection marker in mammalian cells 4266 4771
recombination site attR2 attR2 recombinational cloning site 4127 4142
recombination site attR1 attR1 recombinational cloning site 2440 2456
response element HIV-1 RRE HIV-1 Rev response element (RRE) 1075 1308
selectable marker AmpR Ampicillin resistance gene for selection in bacteria 6728 7588
selectable marker BlastR Blasticidin resistance gene in mammalian cells 4849 5249
selectable marker CmR chloramphenicol resistance marker (removed in plasmids with insert) 2673 3329
splice acceptor site 3' splice acceptor two 3' splice acceptors at 1656 and 1684 1656 1656
splice donor site 5' splice donor 5' splice donor 520 520
tag V5 C-terminal V5 epitope tag 4197 4238

 

  • Gene
  • Protein
  • Clone
  • Organism
  • Genome
  • Reagents

Gene

 

Gene Symbol : TBC1D10B
Symbol Nomenclature : TBC1D10B
SYNONYM : EPI64B
Designation : Rab27A-GAPbeta|TBC1 domain family member 10B|rab27A-GAP-beta
Full Nomenclature : TBC1 domain family, member 10B
GENEID : 26000
Locus Tag : FP2461
HGNC : 24510
MIM : 613620
Vega : OTTHUMG00000132396
Target GenBank: BC093814

 

Reference Sequence Alignment

  

HsCD00329692      ------------------------------------------------------------
NM_015527.3       ATGGAGACGGGCACGGCGCCCCTGGTGGCCCCGCCGCGCCGTCATGGCGCCCCCGCGGCC
                                                                              

HsCD00329692      ------------------------------------------------------------
NM_015527.3       CCCTCGCCGCCGCCCCGGGGTTCCCGGGCCGGGCCCGTCGTGGTGGTGGCTCCGGGACCT
                                                                              

HsCD00329692      ------------------------------------------------------------
NM_015527.3       CCAGTGACTACGGCCACTTCGGCCCCCGTCACCCTGGTGGCCCCCGGGGAGGCGCGGCCC
                                                                              

HsCD00329692      ------------------------------------------------------------
NM_015527.3       GCCTGGGTCCCGGGGTCGGCCGAGACCTCTGCTCCGGCCCCGGCCCCAGCCCCGGCCCCA
                                                                              

HsCD00329692      ------------------------------------------------------------
NM_015527.3       GCCCCGGCTGTCACGGGCAGCACGGTGGTGGTGCTGACCCTGGAGGCCTCGCCCGAAGCC
                                                                              

HsCD00329692      ------------------------------------------------------------
NM_015527.3       CCAAAGCCGCAGCTCCCCTCCGGCCCGGAATCCCCAGAGCCCGCGGCAGTGGCTGGAGTT
                                                                              

HsCD00329692      ------------------------------------------------------------
NM_015527.3       GAGACATCGAGGGCTCTGGCCGCAGGGGCAGACTCGCCGAAGACAGAGGAGGCTCGACCC
                                                                              

HsCD00329692      ------------------------------------------------------------
NM_015527.3       TCACCCGCCCCAGGACCAGGGACCCCCACCGGGACCCCTACCAGGACCCCTTCCAGAACG
                                                                              

HsCD00329692      ------------------------------------------------------------
NM_015527.3       GCTCCTGGTGCCCTGACCGCCAAACCCCCGCTTGCCCCCAAGCCGGGAACCACAGTGGCC
                                                                              

HsCD00329692      ------------------------------------------------------------
NM_015527.3       TCAGGAGTGACTGCACGGAGTGCATCAGGACAAGTGACAGGTGGGCATGGAGCTGCCGCA
                                                                              

HsCD00329692      ------------------------------------------------------------
NM_015527.3       GCAACATCAGCATCAGCAGGACAGGCTCCTGAGGACCCCTCAGGCCCTGGCACAGGCCCC
                                                                              

HsCD00329692      ------------------------------------------------------------
NM_015527.3       TCTGGGACTTGTGAGGCTCCGGTAGCTGTCGTGACCGTGACCCCAGCTCCGGAGCCTGCT
                                                                              

HsCD00329692      ------------------------------------------------------------
NM_015527.3       GAAAACTCTCAAGACCTGGGCTCCACGTCCAGCCTGGGACCTGGCATCTCTGGGCCTCGA
                                                                              

HsCD00329692      ---------------------------------------------ATGTCTGGGACCTTG
NM_015527.3       GGGCAGGCCCCGGACACGCTGAGTTACTTGGACTCCGTGAGCCTCATGTCTGGGACCTTG
                                                               ***************

HsCD00329692      GAGTCCTTGGCGGATGATGTGAGCTCCATGGGCTCAGATTCAGAGATAAACGGGCTGGCC
NM_015527.3       GAGTCCTTGGCGGATGATGTGAGCTCCATGGGCTCAGATTCAGAGATAAACGGGCTGGCC
                  ************************************************************

HsCD00329692      CTGCGCAAGACGGACAAGTATGGCTTCCTTGGGGGCAGCCAGTACTCGGGCAGCCTAGAG
NM_015527.3       CTGCGCAAGACGGACAAGTATGGCTTCCTTGGGGGCAGCCAGTACTCGGGCAGCCTAGAG
                  ************************************************************

HsCD00329692      AGCTCCATTCCCGTGGACGTGGCTCGGCAGCGGGAGCTCAAATGGCTGGACATGTTCAGT
NM_015527.3       AGCTCCATTCCCGTGGACGTGGCTCGGCAGCGGGAGCTCAAATGGCTGGACATGTTCAGT
                  ************************************************************

HsCD00329692      AACTGGGATAAGTGGCTGTCACGGCGATTCCAGAAGGTGAAGCTGCGCTGCCGGAAGGGG
NM_015527.3       AACTGGGATAAGTGGCTGTCACGGCGATTCCAGAAGGTGAAGCTGCGCTGCCGGAAGGGG
                  ************************************************************

HsCD00329692      ATCCCCTCCTCTCTCAGAGCCAAGGCCTGGCAGTACCTGTCTAATAGCAAGGAACTTCTG
NM_015527.3       ATCCCCTCCTCTCTCAGAGCCAAAGCCTGGCAGTACCTGTCTAATAGCAAGGAACTTCTG
                  ***********************.************************************

HsCD00329692      GAGCAGAACCCAGGAAAGTTTGAGGAGCTGGAACGGGCTCCTGGGGACCCCAAGTGGCTG
NM_015527.3       GAGCAGAACCCAGGAAAGTTTGAGGAGCTGGAACGGGCTCCTGGGGACCCCAAGTGGCTG
                  ************************************************************

HsCD00329692      GATGTGATTGAGAAGGACCTGCACCGCCAGTTCCCTTTCCACGAGATGTTTGCTGCTCGA
NM_015527.3       GATGTGATTGAGAAGGACCTGCACCGCCAGTTCCCTTTCCACGAGATGTTTGCTGCTCGA
                  ************************************************************

HsCD00329692      GGGGGGCATGGGCAACAGGACCTGTACCGAATCCTGAAGGCCTACACCATCTACCGGCCT
NM_015527.3       GGGGGGCATGGGCAACAGGACCTGTACCGAATCCTGAAGGCCTACACCATCTACCGGCCT
                  ************************************************************

HsCD00329692      GACGAGGGTTACTGCCAGGCCCAGGCCCCCGTGGCTGCGGTCCTGCTCATGCACATGCCT
NM_015527.3       GACGAGGGTTACTGCCAGGCCCAGGCCCCCGTGGCTGCGGTCCTGCTCATGCACATGCCT
                  ************************************************************

HsCD00329692      GCGGAGCAAGCCTTTTGGTGCCTGGTGCAGATCTGCGACAAGTACCTCCCAGGTTACTAC
NM_015527.3       GCGGAGCAAGCCTTTTGGTGCCTGGTGCAGATCTGCGACAAGTACCTCCCAGGTTACTAC
                  ************************************************************

HsCD00329692      AGTGCAGGGCTGGAGGCCATTCAGCTGGACGGGGAGATCTTTTTTGCACTCCTGCGCCGG
NM_015527.3       AGTGCAGGGCTGGAGGCCATTCAGCTGGACGGGGAGATCTTTTTTGCACTCCTGCGCCGG
                  ************************************************************

HsCD00329692      GCCTCCCCGCTGGCGCATCGCCACCTGCGGCGGCAGCGCATTGACCCTGTGCTCTACATG
NM_015527.3       GCCTCCCCGCTGGCGCATCGCCACCTGCGGCGGCAGCGCATTGACCCTGTGCTCTACATG
                  ************************************************************

HsCD00329692      ACGGAGTGGTTCATGTGCATCTTCGCCCGCACCCTGCCCTGGGCGTCGGTGCTGCGTGTC
NM_015527.3       ACGGAGTGGTTCATGTGCATCTTCGCCCGCACCCTGCCCTGGGCGTCGGTGCTGCGTGTC
                  ************************************************************

HsCD00329692      TGGGACATGTTTTTCTGTGAAGGCGTTAAGATCATCTTCCGGGTGGCCCTGGTCCTGCTG
NM_015527.3       TGGGACATGTTTTTCTGTGAAGGCGTTAAGATCATCTTCCGGGTGGCCCTGGTCCTGCTG
                  ************************************************************

HsCD00329692      CGCCACACGCTGGGCTCAGTGGAGAAGCTGCGCTCCTGCCAAGGCATGTATGAGACCATG
NM_015527.3       CGCCACACGCTGGGCTCAGTGGAGAAGCTGCGCTCCTGCCAAGGCATGTATGAGACCATG
                  ************************************************************

HsCD00329692      GAGCAGCTGCGTAACCTGCCCCAGCAGTGCATGCAGGAAGACTTCCTGGTGCATGAGGTG
NM_015527.3       GAGCAGCTGCGTAACCTGCCCCAGCAGTGCATGCAGGAAGACTTCCTGGTGCATGAGGTG
                  ************************************************************

HsCD00329692      ACCAATCTGCCGGTGACAGAAGCACTGATTGAGCGGGAGAATGCAGCCCAGCTCAAGAAG
NM_015527.3       ACCAATCTGCCGGTGACAGAAGCACTGATTGAGCGGGAGAATGCAGCCCAGCTCAAGAAG
                  ************************************************************

HsCD00329692      TGGCGGGAAACGCGGGGGGAGCTGCAGTATCGGCCCTCACGGCGACTGCATGGGTCCCGG
NM_015527.3       TGGCGGGAAACGCGGGGGGAGCTGCAGTATCGGCCCTCACGGCGACTGCATGGGTCCCGG
                  ************************************************************

HsCD00329692      GCCATCCACGAGGAGCGCCGGCGGCAACAGCCACCCCTGGGCCCCTCCTCCAGCCTCCTC
NM_015527.3       GCCATCCACGAGGAGCGCCGGCGGCAACAGCCACCCCTGGGCCCCTCCTCCAGCCTCCTC
                  ************************************************************

HsCD00329692      AGCCTCCCTGGCCTCAAGAGCCGAGGCTCCCGGGCAGCTGGAGGGGCCCCGTCCCCGCCG
NM_015527.3       AGCCTCCCTGGCCTCAAGAGCCGAGGCTCCCGGGCAGCTGGAGGGGCCCCGTCCCCGCCG
                  ************************************************************

HsCD00329692      CCCCCCGTCCGCAGAGCCAGTGCTGGGCCTGCCCCAGGGCCTGTGGTCACTGCTGAGGGA
NM_015527.3       CCCCCCGTCCGCAGAGCCAGTGCTGGGCCTGCCCCAGGGCCTGTGGTCACTGCTGAGGGA
                  ************************************************************

HsCD00329692      CTGCATCCATCCCTTCCCTCACCCACTGGCAATAGCACCCCCTTGGGTTCCAGCAAGGAG
NM_015527.3       CTGCATCCATCCCTTCCCTCACCCACTGGCAATAGCACCCCCTTGGGTTCCAGCAAGGAG
                  ************************************************************

HsCD00329692      ACCCGGAAGCAGGAGAAGGAGCGGCAGAAACAGGAGAAGGAGCGGCAGAAACAGGAGAAG
NM_015527.3       ACCCGGAAGCAGGAGAAGGAGCGGCAGAAACAGGAGAAGGAGCGGCAGAAACAGGAGAAG
                  ************************************************************

HsCD00329692      GAGCGGGAGAAGGAGCGGCAGAAGCAGGAGAAAGAGCGAGAGAAGCAGGAAAAGGAGCGA
NM_015527.3       GAGCGGGAGAAGGAGCGGCAGAAGCAGGAGAAAGAGCGAGAGAAGCAGGAAAAGGAGCGA
                  ************************************************************

HsCD00329692      GAGAAGCAGGAGAAGGAGCGGCAGAAGCAGGAGAAGAAGGCTCAAGGCCGGAAGCTTTCG
NM_015527.3       GAGAAGCAGGAGAAGGAGCGGCAGAAGCAGGAGAAGAAGGCTCAAGGCCGGAAGCTTTCG
                  ************************************************************

HsCD00329692      CTGCGTCGAAAGGCAGATGGGCCCCCAGGCCCCCATGATGGTGGGGACAGGCCCTCAGCC
NM_015527.3       CTGCGTCGAAAGGCAGATGGGCCCCCAGGCCCCCATGATGGTGGGGACAGGCCCTCAGCC
                  ************************************************************

HsCD00329692      GAGGCCCGGCAGGACGCTTACTTCTTG
NM_015527.3       GAGGCCCGGCAGGACGCTTACTTCTGA
                  ************************* .

Clone

Cloning Information : 268149
HIP Master Clone ID : 193191
Original Clone ID : FLH268149.01L

Organism


TAX_ID : 9606
Species Specific ID: 26000

Genome

Chromosome : 16
Map Location : 16p11.2
Ensembl : ENSG00000169221

Reagents

Labome : TBC1D10B-antibody