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CREBZF (Homo sapiens) in pLenti6.2/V5-DEST (V5-tagged lentiviral mammalian expression vector)

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Explanation of Terms


Gene: Gene Symbol:  CREBZF
Gene Name:  CREB/ATF bZIP transcription factor
Sequence             
Original Clone ID: FLH268151.01L
Keyword: None
Species: Homo sapiens
Type: cDNA
Vector Name: pLenti6.2/V5-DEST               Format:  FUSION
Source: HIP
Description: None
Comments: None
Mutation/
Discrepancy :
No / No
Publications: None
Authors:
HsCD00329694

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AVAILABLE
No restriction
Special MTA: None

 

Insert sequence: 819nts         Open reading frame : 1 to 819
Reference Sequence Alignment

ATGGAGGAGGAGGCGATCGCCAGCCTCCCGGGGGAAGAGACGGAGGATATGGACTTTCTG
TCTGGGCTGGAACTGGCGGATCTCCTGGACCCCAGGCAACCGGACTGGCACCTGGACCCC
GGGCTTAGCTCGCCGGGGCCTCTCTCCTCGTCTGGCGGAGGCTCGGATAGCGGCGGCCTG
TGGAGAGGGGACGATGACGATGAGGCCGCGGCTGCTGAAATGCAGCGCTTCTCTGACCTG
CTGCAAAGGCTGTTAAACGGTATCGGAGGCTGCAGCAGCAGCAGTGACAGTGGCAGCGCC
GAAAAGAGGCGGAGAAAGTCCCCAGGAGGAGGCGGCGGTGGCGGCAGCGGTAACGACAAC
AACCAGGCGGCGACAAAGAGTCCCCGGAAGGCGGCGGCGGCCGCTGCCCGCCTTAATCGA
CTGAAGAAGAAGGAGTACGTGATGGGGCTGGAGAGTCGAGTCCGGGGTCTGGCAGCCGAG
AACCAGGAGCTGCGGGCCGAGAATCGGGAGCTGGGCAAACGCGTACAGGCACTGCAGGAG
GAGAGTCGCTACCTACGGGCAGTCTTAGCCAACGAGACTGGACTGGCTCGCTTGCTGAGC
CGGCTGAGCGGCGTGGGACTGCGGCTGACCACCTCGCTCTTCAGAGACTCGCCCGCCGGT
GACCACGACTACGCTCTGCCGGTGGGAAAGCAGAAGCAGGACCTGCTGGAAGAGGACGAC
TCGGCGGGAGGAGTCTGTCTCCATGTGGACAAGGATAAGGTGTCGGTGGAGTTCTGCTCG
GCGTGCGCCCGGAAGGCGTCGTCTTCTCTTAAAATGTTG


Coding Sequence Details
Insert Sequence Verified?: N Verification Method: Insert was fully sequenced in parent vector
Reference Sequence Annotations:
End on reference sequence 907
Start on reference sequence 89
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
5p GTACAAAAAAGCAGGCTCCACC(atg-goi)

3' Linker Sequence:
5p (goi-stop)GACCCAGCTTTCTTGTAC

 

 Recommended Growth Condition:

Distributed in bacterial strain : DH5-alpha T1 phage resistant
Antibiotic Selection: Host Type:  bacterial    Marker:  ampicillin
Host Type:  mammalian    Marker:  blasticidin
Bacterial Selection Condition:  100 ug/mL ampicillin
Growth Condition:  Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:  Commonly used conditions for ampicillin resistant plasmid clones.
Protein Expression Results: None
Recommended expression in: Not Applicable
       DyNA Vector Map

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Vector Name: pLenti6.2/V5-DEST

pLenti6.2V5-DEST in advanced viewed

Synonyms: pLenti6.2/V5-DEST
Sequencing Primer: Forward:  CMV forward
Reverse:  V5 reverse
Description: Lentiviral mammalian expression vector;CMV promoter and C-terminal V5 tag; ampicillin resistance in bacteria; blasticidin resistance in mammalian cells; Gateway recombinational cloning.
Comments: None
Size (bp): 8813
Parent Vector: None
Empty Vector: None
Properties: Gateway, acceptor (destination), lentiviral infection, mammalian expression, recombinational cloning, with tag/fusion/marker
Author Name: Invitrogen
Publications: None
Vector Map:         Vector Sequence:
Sequence:

 



Vector Features:

Type Name Description Start Position End Position
bacterial origin pUC ori pUC origin of replication 7733 8406
death cassette ccdB ccdB death cassette (removed when insert is present) 3767 3976
packaging signal HIV-1 psi HIV-1 psi packaging signal 521 565
poly-A signal SV40 SV40 polyadenylation signal 5639 5770
promoter RSV RSV/5' LTR hybrid promoter 1 410
promoter CMV CMV promoter driving gene of interest 1809 2392
promoter PGK PGK promoter to drive selection marker in mammalian cells 4266 4771
recombination site attR2 attR2 recombinational cloning site 4127 4142
recombination site attR1 attR1 recombinational cloning site 2440 2456
response element HIV-1 RRE HIV-1 Rev response element (RRE) 1075 1308
selectable marker AmpR Ampicillin resistance gene for selection in bacteria 6728 7588
selectable marker BlastR Blasticidin resistance gene in mammalian cells 4849 5249
selectable marker CmR chloramphenicol resistance marker (removed in plasmids with insert) 2673 3329
splice acceptor site 3' splice acceptor two 3' splice acceptors at 1656 and 1684 1656 1656
splice donor site 5' splice donor 5' splice donor 520 520
tag V5 C-terminal V5 epitope tag 4197 4238

 

  • Gene
  • Protein
  • Clone
  • Organism
  • Genome
  • Reagents

Gene

 

Gene Symbol : CREBZF
Symbol Nomenclature : CREBZF
SYNONYM : SMILE; ZF
Designation : HCF-binding transcription factor Zhangfei|SHP-interacting leucine zipper protein|Zhangfei|host cell factor-binding transcription factor Zhangfei
Full Nomenclature : CREB/ATF bZIP transcription factor
GENEID : 58487
HGNC : 24905
MIM : 606444
Vega : OTTHUMG00000133648
Target GenBank: BC093798

 

Reference Sequence Alignment

  

HsCD00329694        ------------------------------------------------------------
XM_005274138.1      ATGAGGCATAGCCTGACCAAGCTGCTGGCAGCCTCGGGCAGCAACTCCCCAACCCGCAGT
NM_001039618.2      ATGAGGCATAGCCTGACCAAGCTGCTGGCAGCCTCGGGCAGCAACTCCCCAACCCGCAGT
XM_005274135.1      ATGAGGCATAGCCTGACCAAGCTGCTGGCAGCCTCGGGCAGCAACTCCCCAACCCGCAGT
XM_005274136.1      ATGAGGCATAGCCTGACCAAGCTGCTGGCAGCCTCGGGCAGCAACTCCCCAACCCGCAGT
                                                                                

HsCD00329694        ------------------------------------------------------------
XM_005274138.1      GAGAGCCCGGAGCCGGCTGCAACTTGTTCGCTGCCCTCTGACCTGACCCGGGCTGCAGCG
NM_001039618.2      GAGAGCCCGGAGCCGGCTGCAACTTGTTCGCTGCCCTCTGACCTGACCCGGGCTGCAGCG
XM_005274135.1      GAGAGCCCGGAGCCGGCTGCAACTTGTTCGCTGCCCTCTGACCTGACCCGGGCTGCAGCG
XM_005274136.1      GAGAGCCCGGAGCCGGCTGCAACTTGTTCGCTGCCCTCTGACCTGACCCGGGCTGCAGCG
                                                                                

HsCD00329694        ------------------------------------------------------------
XM_005274138.1      GGGGAGGAGGAGACGGCGGCGGCCGGATCTCCCGGCCGCAAGCAGCAGTTTGGCGACGAA
NM_001039618.2      GGGGAGGAGGAGACGGCGGCGGCCGGATCTCCCGGCCGCAAGCAGCAGTTTGGCGACGAA
XM_005274135.1      GGGGAGGAGGAGACGGCGGCGGCCGGATCTCCCGGCCGCAAGCAGCAGTTTGGCGACGAA
XM_005274136.1      GGGGAGGAGGAGACGGCGGCGGCCGGATCTCCCGGCCGCAAGCAGCAGTTTGGCGACGAA
                                                                                

HsCD00329694        ------------------------------------------------------------
XM_005274138.1      GGAGAGTTGGAAGCCGGGAGGGGGAGCCGCGGCGGCGTGGCCGTGCGCGCGCCCTCCCCC
NM_001039618.2      GGAGAGTTGGAAGCCGGGAGGGGGAGCCGCGGCGGCGTGGCCGTGCGCGCGCCCTCCCCC
XM_005274135.1      GGAGAGTTGGAAGCCGGGAGGGGGAGCCGCGGCGGCGTGGCCGTGCGCGCGCCCTCCCCC
XM_005274136.1      GGAGAGTTGGAAGCCGGGAGGGGGAGCCGCGGCGGCGTGGCCGTGCGCGCGCCCTCCCCC
                                                                                

HsCD00329694        ------ATGGAGGAGGAGGCGATCGCCAGCCTCCCGGGGGAAGAGACGGAGGATATGGAC
XM_005274138.1      GAGGAGATGGAGGAGGAGGCGATCGCCAGCCTCCCGGGGGAAGAGACGGAGGATATGGAC
NM_001039618.2      GAGGAGATGGAGGAGGAGGCGATCGCCAGCCTCCCGGGGGAAGAGACGGAGGATATGGAC
XM_005274135.1      GAGGAGATGGAGGAGGAGGCGATCGCCAGCCTCCCGGGGGAAGAGACGGAGGATATGGAC
XM_005274136.1      GAGGAGATGGAGGAGGAGGCGATCGCCAGCCTCCCGGGGGAAGAGACGGAGGATATGGAC
                          ******************************************************

HsCD00329694        TTTCTGTCTGGGCTGGAACTGGCGGATCTCCTGGACCCCAGGCAACCGGACTGGCACCTG
XM_005274138.1      TTTCTGTCTGGGCTGGAACTGGCGGATCTCCTGGACCCCAGGCAACCGGACTGGCACCTG
NM_001039618.2      TTTCTGTCTGGGCTGGAACTGGCGGATCTCCTGGACCCCAGGCAACCGGACTGGCACCTG
XM_005274135.1      TTTCTGTCTGGGCTGGAACTGGCGGATCTCCTGGACCCCAGGCAACCGGACTGGCACCTG
XM_005274136.1      TTTCTGTCTGGGCTGGAACTGGCGGATCTCCTGGACCCCAGGCAACCGGACTGGCACCTG
                    ************************************************************

HsCD00329694        GACCCCGGGCTTAGCTCGCCGGGGCCTCTCTCCTCGTCTGGCGGAGGCTCGGATAGCGGC
XM_005274138.1      GACCCCGGGCTTAGCTCGCCGGGGCCTCTCTCCTCGTCTGGCGGAGGCTCGGATAGCGGC
NM_001039618.2      GACCCCGGGCTTAGCTCGCCGGGGCCTCTCTCCTCGTCTGGCGGAGGCTCGGATAGCGGC
XM_005274135.1      GACCCCGGGCTTAGCTCGCCGGGGCCTCTCTCCTCGTCTGGCGGAGGCTCGGATAGCGGC
XM_005274136.1      GACCCCGGGCTTAGCTCGCCGGGGCCTCTCTCCTCGTCTGGCGGAGGCTCGGATAGCGGC
                    ************************************************************

HsCD00329694        GGCCTGTGGAGAGGGGACGATGACGATGAGGCCGCGGCTGCTGAAATGCAGCGCTTCTCT
XM_005274138.1      GGCCTGTGGAGAGGGGACGATGACGATGAGGCCGCGGCTGCTGAAATGCAGCGCTTCTCT
NM_001039618.2      GGCCTGTGGAGAGGGGACGATGACGATGAGGCCGCGGCTGCTGAAATGCAGCGCTTCTCT
XM_005274135.1      GGCCTGTGGAGAGGGGACGATGACGATGAGGCCGCGGCTGCTGAAATGCAGCGCTTCTCT
XM_005274136.1      GGCCTGTGGAGAGGGGACGATGACGATGAGGCCGCGGCTGCTGAAATGCAGCGCTTCTCT
                    ************************************************************

HsCD00329694        GACCTGCTGCAAAGGCTGTTAAACGGTATCGGAGGCTGCAGCAGCAGCAGTGACAGTGGC
XM_005274138.1      GACCTGCTGCAAAGGCTGTTAAACGGTATCGGAGGCTGCAGCAGCAGCAGTGACAGTGGC
NM_001039618.2      GACCTGCTGCAAAGGCTGTTAAACGGTATCGGAGGCTGCAGCAGCAGCAGTGACAGTGGC
XM_005274135.1      GACCTGCTGCAAAGGCTGTTAAACGGTATCGGAGGCTGCAGCAGCAGCAGTGACAGTGGC
XM_005274136.1      GACCTGCTGCAAAGGCTGTTAAACGGTATCGGAGGCTGCAGCAGCAGCAGTGACAGTGGC
                    ************************************************************

HsCD00329694        AGCGCCGAAAAGAGGCGGAGAAAGTCCCCAGGAGGAGGCGGCGGTGGCGGCAGCGGTAAC
XM_005274138.1      AGCGCCGAAAAGAGGCGGAGAAAGTCCCCAGGAGGAGGCGGCGGTGGCGGCAGCGGTAAC
NM_001039618.2      AGCGCCGAAAAGAGGCGGAGAAAGTCCCCAGGAGGAGGCGGCGGTGGCGGCAGCGGTAAC
XM_005274135.1      AGCGCCGAAAAGAGGCGGAGAAAGTCCCCAGGAGGAGGCGGCGGTGGCGGCAGCGGTAAC
XM_005274136.1      AGCGCCGAAAAGAGGCGGAGAAAGTCCCCAGGAGGAGGCGGCGGTGGCGGCAGCGGTAAC
                    ************************************************************

HsCD00329694        GACAACAACCAGGCGGCGACAAAGAGTCCCCGGAAGGCGGCGGCGGCCGCTGCCCGCCTT
XM_005274138.1      GACAACAACCAGGCGGCGACAAAGAGTCCCCGGAAGGCGGCGGCGGCCGCTGCCCGCCTT
NM_001039618.2      GACAACAACCAGGCGGCGACAAAGAGTCCCCGGAAGGCGGCGGCGGCCGCTGCCCGCCTT
XM_005274135.1      GACAACAACCAGGCGGCGACAAAGAGTCCCCGGAAGGCGGCGGCGGCCGCTGCCCGCCTT
XM_005274136.1      GACAACAACCAGGCGGCGACAAAGAGTCCCCGGAAGGCGGCGGCGGCCGCTGCCCGCCTT
                    ************************************************************

HsCD00329694        AATCGACTGAAGAAGAAGGAGTACGTGATGGGGCTGGAGAGTCGAGTCCGGGGTCTGGCA
XM_005274138.1      AATCGACTGAAGAAGAAGGAGTACGTGATGGGGCTGGAGAGTCGAGTCCGGGGTCTGGCA
NM_001039618.2      AATCGACTGAAGAAGAAGGAGTACGTGATGGGGCTGGAGAGTCGAGTCCGGGGTCTGGCA
XM_005274135.1      AATCGACTGAAGAAGAAGGAGTACGTGATGGGGCTGGAGAGTCGAGTCCGGGGTCTGGCA
XM_005274136.1      AATCGACTGAAGAAGAAGGAGTACGTGATGGGGCTGGAGAGTCGAGTCCGGGGTCTGGCA
                    ************************************************************

HsCD00329694        GCCGAGAACCAGGAGCTGCGGGCCGAGAATCGGGAGCTGGGCAAACGCGTACAGGCACTG
XM_005274138.1      GCCGAGAACCAGGAGCTGCGGGCCGAGAATCGGGAGCTGGGCAAACGCGTACAGGCACTG
NM_001039618.2      GCCGAGAACCAGGAGCTGCGGGCCGAGAATCGGGAGCTGGGCAAACGCGTACAGGCACTG
XM_005274135.1      GCCGAGAACCAGGAGCTGCGGGCCGAGAATCGGGAGCTGGGCAAACGCGTACAGGCACTG
XM_005274136.1      GCCGAGAACCAGGAGCTGCGGGCCGAGAATCGGGAGCTGGGCAAACGCGTACAGGCACTG
                    ************************************************************

HsCD00329694        CAGGAGGAGAGTCGCTACCTACGGGCAGTCTTAGCCAACGAGACTGGACTGGCTCGCTTG
XM_005274138.1      CAGGAGGAGAGTCGCTACCTACGGGCAGTCTTAGCCAACGAGACTGGACTGGCTCGCTTG
NM_001039618.2      CAGGAGGAGAGTCGCTACCTACGGGCAGTCTTAGCCAACGAGACTGGACTGGCTCGCTTG
XM_005274135.1      CAGGAGGAGAGTCGCTACCTACGGGCAGTCTTAGCCAACGAGACTGGACTGGCTCGCTTG
XM_005274136.1      CAGGAGGAGAGTCGCTACCTACGGGCAGTCTTAGCCAACGAGACTGGACTGGCTCGCTTG
                    ************************************************************

HsCD00329694        CTGAGCCGGCTGAGCGGCGTGGGACTGCGGCTGACCACCTCGCTCTTCAGAGACTCGCCC
XM_005274138.1      CTGAGCCGGCTGAGCGGCGTGGGACTGCGGCTGACCACCTCGCTCTTCAGAGACTCGCCC
NM_001039618.2      CTGAGCCGGCTGAGCGGCGTGGGACTGCGGCTGACCACCTCGCTCTTCAGAGACTCGCCC
XM_005274135.1      CTGAGCCGGCTGAGCGGCGTGGGACTGCGGCTGACCACCTCGCTCTTCAGAGACTCGCCC
XM_005274136.1      CTGAGCCGGCTGAGCGGCGTGGGACTGCGGCTGACCACCTCGCTCTTCAGAGACTCGCCC
                    ************************************************************

HsCD00329694        GCCGGTGACCACGACTACGCTCTGCCGGTGGGAAAGCAGAAGCAGGACCTGCTGGAAGAG
XM_005274138.1      GCCGGTGACCACGACTACGCTCTGCCGGTGGGAAAGCAGAAGCAGGACCTGCTGGAAGAG
NM_001039618.2      GCCGGTGACCACGACTACGCTCTGCCGGTGGGAAAGCAGAAGCAGGACCTGCTGGAAGAG
XM_005274135.1      GCCGGTGACCACGACTACGCTCTGCCGGTGGGAAAGCAGAAGCAGGACCTGCTGGAAGAG
XM_005274136.1      GCCGGTGACCACGACTACGCTCTGCCGGTGGGAAAGCAGAAGCAGGACCTGCTGGAAGAG
                    ************************************************************

HsCD00329694        GACGACTCGGCGGGAGGAGTCTGTCTCCATGTGGACAAGGATAAGGTGTCGGTGGAGTTC
XM_005274138.1      GACGACTCGGCGGGAGGAGTCTGTCTCCATGTGGACAAGGATAAGGTGTCGGTGGAGTTC
NM_001039618.2      GACGACTCGGCGGGAGGAGTCTGTCTCCATGTGGACAAGGATAAGGTGTCGGTGGAGTTC
XM_005274135.1      GACGACTCGGCGGGAGGAGTCTGTCTCCATGTGGACAAGGATAAGGTGTCGGTGGAGTTC
XM_005274136.1      GACGACTCGGCGGGAGGAGTCTGTCTCCATGTGGACAAGGATAAGGTGTCGGTGGAGTTC
                    ************************************************************

HsCD00329694        TGCTCGGCGTGCGCCCGGAAGGCGTCGTCTTCTCTTAAAATGTTG------------
XM_005274138.1      TGCTCGGCGTGCGCCCGGAAGGCGTCGTCTTCTCTTAAAATTTTCTTTTTTAGGTGA
NM_001039618.2      TGCTCGGCGTGCGCCCGGAAGGCGTCGTCTTCTCTTAAAATGTAG------------
XM_005274135.1      TGCTCGGCGTGCGCCCGGAAGGCGTCGTCTTCTCTTAAAATTTTCTTTTTTAGGTGA
XM_005274136.1      TGCTCGGCGTGCGCCCGGAAGGCGTCGTCTTCTCTTAAAATTTTCTTTTTTAGGTGA
                    ***************************************** *:             

Protein

SMART domain : BRLZ : basic region leucin zipper
UniProt : Q9NS37
HPRD : 07340

Clone

Cloning Information : 268151
HIP Master Clone ID : 193193
Original Clone ID : FLH268151.01L

Organism


TAX_ID : 9606
Species Specific ID: 58487

Genome

Chromosome : 11
Map Location : 11q14
Ensembl : ENSG00000137504

Reagents

Labome : Zhangfei-antibody