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SMYD5 (Homo sapiens) in pLenti6.2/V5-DEST (V5-tagged lentiviral mammalian expression vector)

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Explanation of Terms


Gene: Gene Symbol:  SMYD5
Gene Name:  SMYD family member 5
Sequence             
Original Clone ID: FLH268155.01L
Keyword: None
Species: Homo sapiens
Type: cDNA
Vector Name: pLenti6.2/V5-DEST               Format:  FUSION
Source: HIP
Description: None
Comments: None
Mutation/
Discrepancy :
No / No
Publications: None
Authors:
HsCD00329698

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AVAILABLE
No restriction
Special MTA: None

 

Insert sequence: 909nts         Open reading frame : 1 to 909
Reference Sequence Alignment

ATGTACTGCAGTGCAGAATGTCGGTTGGCAGCCACTGAGCAATACCACCAGGTCCTGTGC
CCAGGCCCCTCCCAGGATGACCCCTTGCATCCTCTCAATAAGCTTCAGGAGGCATGGAGG
AGTATTCACTACCCACCTGAGACTGCAAGCATCATGTTGATGGCTAGGATGGTGGCCACA
GTGAAGCAGGCGAAGGACAAGGACCGTTGGATCAGACTCTTTTCCCAGTTTTGTAACAAA
ACAGCCAATGAAGAGGAGGAAATTGTCCATAAACTTCTGGGAGACAAATTCAAGGGCCAA
CTGGAACTTCTGCGGAGACTCTTCACAGAGGCCCTCTATGAGGAAGCAGTCAGCCAGTGG
TTCACTCCAGATGGATTCCGGTCTCTCTTTGCTCTTGTTGGGACCAATGGCCAAGGAATC
GGGACCAGCTCCCTAAGCCAGTGGGTCCATGCCTGTGACACTCTGGAGTTGAAGCCTCAG
GACCGTGAGCAGCTTGACGCCTTCATTGACCAGCTATACAAGGACATCGAGGCAGCAACT
GGAGAGTTTCTTAACTGTGAAGGATCTGGCCTCTTTGTGCTTCAGAGCTGCTGCAACCAC
AGTTGTGTGCCCAATGCAGAGACCTCCTTTCCAGAAAACAACTTCCTTTTGCATGTCACT
GCTCTGGAGGATATTAAGCCAGGAGAGGAAATTTGTATCAGCTACTTGGACTGCTGTCAG
CGGGAGCGCAGCCGCCACAGCCGCCACAAGATCCTCAGGGAGAACTATCTATTTGTCTGT
TCCTGTCCCAAATGCCTGGCAGAGGCTGATGAACCCAATGTGACCTCAGAAGAGGAAGAG
GAAGAGGAGGAGGAGGAGGAAGGAGAGCCAGAAGATGCAGAGCTGGGGGATGAGATGACT
GATGTGTTG


Coding Sequence Details
Insert Sequence Verified?: N Verification Method: Insert was fully sequenced in parent vector
Reference Sequence Annotations:
End on reference sequence 1262
Start on reference sequence 354
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
5p GTACAAAAAAGCAGGCTCCACC(atg-goi)

3' Linker Sequence:
5p (goi-stop)GACCCAGCTTTCTTGTAC

 

 Recommended Growth Condition:

Distributed in bacterial strain : DH5-alpha T1 phage resistant
Antibiotic Selection: Host Type:  bacterial    Marker:  ampicillin
Host Type:  mammalian    Marker:  blasticidin
Bacterial Selection Condition:  100 ug/mL ampicillin
Growth Condition:  Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:  Commonly used conditions for ampicillin resistant plasmid clones.
Protein Expression Results: None
Recommended expression in: Not Applicable
       DyNA Vector Map

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Vector Name: pLenti6.2/V5-DEST

pLenti6.2V5-DEST in advanced viewed

Synonyms: pLenti6.2/V5-DEST
Sequencing Primer: Forward:  CMV forward
Reverse:  V5 reverse
Description: Lentiviral mammalian expression vector;CMV promoter and C-terminal V5 tag; ampicillin resistance in bacteria; blasticidin resistance in mammalian cells; Gateway recombinational cloning.
Comments: None
Size (bp): 8813
Parent Vector: None
Empty Vector: None
Properties: Gateway, acceptor (destination), lentiviral infection, mammalian expression, recombinational cloning, with tag/fusion/marker
Author Name: Invitrogen
Publications: None
Vector Map:         Vector Sequence:
Sequence:

 



Vector Features:

Type Name Description Start Position End Position
bacterial origin pUC ori pUC origin of replication 7733 8406
death cassette ccdB ccdB death cassette (removed when insert is present) 3767 3976
packaging signal HIV-1 psi HIV-1 psi packaging signal 521 565
poly-A signal SV40 SV40 polyadenylation signal 5639 5770
promoter RSV RSV/5' LTR hybrid promoter 1 410
promoter CMV CMV promoter driving gene of interest 1809 2392
promoter PGK PGK promoter to drive selection marker in mammalian cells 4266 4771
recombination site attR2 attR2 recombinational cloning site 4127 4142
recombination site attR1 attR1 recombinational cloning site 2440 2456
response element HIV-1 RRE HIV-1 Rev response element (RRE) 1075 1308
selectable marker AmpR Ampicillin resistance gene for selection in bacteria 6728 7588
selectable marker BlastR Blasticidin resistance gene in mammalian cells 4849 5249
selectable marker CmR chloramphenicol resistance marker (removed in plasmids with insert) 2673 3329
splice acceptor site 3' splice acceptor two 3' splice acceptors at 1656 and 1684 1656 1656
splice donor site 5' splice donor 5' splice donor 520 520
tag V5 C-terminal V5 epitope tag 4197 4238

 

  • Gene
  • Protein
  • Clone
  • Organism
  • Genome
  • Reagents

Gene

 

Gene Symbol : SMYD5
Symbol Nomenclature : SMYD5
SYNONYM : NN8-4AG; RAI15; RRG1; ZMYND23
Designation : SET and MYND domain-containing protein 5|retinoic acid induced 15|retinoic acid responsive|retinoic acid-induced protein 15
Full Nomenclature : SMYD family member 5
GENEID : 10322
HGNC : 16258
Vega : OTTHUMG00000150482
Target GenBank: BC073806

 

Reference Sequence Alignment

  

HsCD00329698        ------------------------------------------------------------
NM_006062.2         ---------ATGGCGGCCTCCATGTGCGACGTGTTCTCCTTCTGCGTGGGCGTGGCGGGC
XM_005264082.1      ATGAGGAGAGAGGCCAAGATCCTTCACGAGGGTCCTTGGAGTGCTGAGGAATTTGGACCA
                                                                                

HsCD00329698        ------------------------------------------------------------
NM_006062.2         CGCGCGCGGGTCTCCGTGGAAGTCCGTTTCGTGAGCAGCGCCAAGGGAAAGGGGCTGTTT
XM_005264082.1      GAGTCCAAAGGCCGTGGGGGCATTGGGAGAGTTTTTAGCAGGAAGGGAAAGGGGCTGTTT
                                                                                

HsCD00329698        ------------------------------------------------------------
NM_006062.2         GCCACACAGCTCATCCGGAAGGGGGAGACCATCTTCGTAGAACGGCCCCTGGTGGCTGCA
XM_005264082.1      GCCACACAGCTCATCCGGAAGGGGGAGACCATCTTCGTAGAACGGCCCCTGGTGGCTGCA
                                                                                

HsCD00329698        ------------------------------------------------------------
NM_006062.2         CAGTTTCTCTGGAATGCACTTTATCGCTACCGAGCCTGTGACCACTGCCTTAGGGCACTA
XM_005264082.1      CAGTTTCTCTGGAATGCACTTTATCGCTACCGAGCCTGTGACCACTGCCTTAGGGCACTA
                                                                                

HsCD00329698        ------------------------------------------------------------
NM_006062.2         GAGAAGGCAGAGGAGAATGCCCAGAGGCTGACCGGGAAACCAGGCCAGGTTCTGCCTCAC
XM_005264082.1      GAGAAGGCAGAGGAGAATGCCCAGAGGCTGACCGGGAAACCAGGCCAGGTTCTGCCTCAC
                                                                                

HsCD00329698        ---------------------------------------------------------ATG
NM_006062.2         CCAGAGCTGTGCACTGTGCGCAAAGACCTCCACCAGAACTGTCCCCATTGCCAAGTGATG
XM_005264082.1      CCAGAGCTGTGCACTGTGCGCAAAGACCTCCACCAGAACTGTCCCCATTGCCAAGTGATG
                                                                             ***

HsCD00329698        TACTGCAGTGCAGAATGTCGGTTGGCAGCCACTGAGCAATACCACCAGGTCCTGTGCCCA
NM_006062.2         TACTGCAGTGCAGAATGTCGGTTGGCAGCCACTGAGCAATACCACCAGGTCCTGTGCCCA
XM_005264082.1      TACTGCAGTGCAGAATGTCGGTTGGCAGCCACTGAGCAATACCACCAGGTCCTGTGCCCA
                    ************************************************************

HsCD00329698        GGCCCCTCCCAGGATGACCCCTTGCATCCTCTCAATAAGCTTCAGGAGGCATGGAGGAGT
NM_006062.2         GGCCCCTCCCAGGATGACCCCTTGCATCCTCTCAATAAGCTTCAGGAGGCATGGAGGAGT
XM_005264082.1      GGCCCCTCCCAGGATGACCCCTTGCATCCTCTCAATAAGCTTCAGGAGGCATGGAGGAGT
                    ************************************************************

HsCD00329698        ATTCACTACCCACCTGAGACTGCAAGCATCATGTTGATGGCTAGGATGGTGGCCACAGTG
NM_006062.2         ATTCACTACCCACCTGAGACTGCAAGCATCATGTTGATGGCTAGGATGGTGGCCACAGTG
XM_005264082.1      ATTCACTACCCACCTGAGACTGCAAGCATCATGTTGATGGCTAGGATGGTGGCCACAGTG
                    ************************************************************

HsCD00329698        AAGCAGGCGAAGGACAAGGACCGTTGGATCAGACTCTTTTCCCAGTTTTGTAACAAAACA
NM_006062.2         AAGCAGGCGAAGGACAAGGACCGTTGGATCAGACTCTTTTCCCAGTTTTGTAACAAAACA
XM_005264082.1      AAGCAGGCGAAGGACAAGGACCGTTGGATCAGACTCTTTTCCCAGTTTTGTAACAAAACA
                    ************************************************************

HsCD00329698        GCCAATGAAGAGGAGGAAATTGTCCATAAACTTCTGGGAGACAAATTCAAGGGCCAACTG
NM_006062.2         GCCAATGAAGAGGAGGAAATTGTCCATAAACTTCTGGGAGACAAATTCAAGGGCCAACTG
XM_005264082.1      GCCAATGAAGAGGAGGAAATTGTCCATAAACTTCTGGGAGACAAATTCAAGGGCCAACTG
                    ************************************************************

HsCD00329698        GAACTTCTGCGGAGACTCTTCACAGAGGCCCTCTATGAGGAAGCAGTCAGCCAGTGGTTC
NM_006062.2         GAACTTCTGCGGAGACTCTTCACAGAGGCCCTCTATGAGGAAGCAGTCAGCCAGTGGTTC
XM_005264082.1      GAACTTCTGCGGAGACTCTTCACAGAGGCCCTCTATGAGGAAGCAGTCAGCCAGTGGTTC
                    ************************************************************

HsCD00329698        ACTCCAGATGGATTCCGGTCTCTCTTTGCTCTTGTTGGGACCAATGGCCAAGGAATCGGG
NM_006062.2         ACTCCAGATGGATTCCGGTCTCTCTTTGCTCTTGTTGGGACCAATGGCCAAGGAATCGGG
XM_005264082.1      ACTCCAGATGGATTCCGGTCTCTCTTTGCTCTTGTTGGGACCAATGGCCAAGGAATCGGG
                    ************************************************************

HsCD00329698        ACCAGCTCCCTAAGCCAGTGGGTCCATGCCTGTGACACTCTGGAGTTGAAGCCTCAGGAC
NM_006062.2         ACCAGCTCCCTAAGCCAGTGGGTCCATGCCTGTGACACTCTGGAGTTGAAGCCTCAGGAC
XM_005264082.1      ACCAGCTCCCTAAGCCAGTGGGTCCATGCCTGTGACACTCTGGAGTTGAAGCCTCAGGAC
                    ************************************************************

HsCD00329698        CGTGAGCAGCTTGACGCCTTCATTGACCAGCTATACAAGGACATCGAGGCAGCAACTGGA
NM_006062.2         CGTGAGCAGCTTGACGCCTTCATTGACCAGCTATACAAGGACATCGAGGCAGCAACTGGA
XM_005264082.1      CGTGAGCAGCTTGACGCCTTCATTGACCAGCTATACAAGGACATCGAGGCAGCAACTGGA
                    ************************************************************

HsCD00329698        GAGTTTCTTAACTGTGAAGGATCTGGCCTCTTTGTGCTTCAGAGCTGCTGCAACCACAGT
NM_006062.2         GAGTTTCTTAACTGTGAAGGATCTGGCCTCTTTGTGCTTCAGAGCTGCTGCAACCACAGT
XM_005264082.1      GAGTTTCTTAACTGTGAAGGATCTGGCCTCTTTGTGCTTCAGAGCTGCTGCAACCACAGT
                    ************************************************************

HsCD00329698        TGTGTGCCCAATGCAGAGACCTCCTTTCCAGAAAACAACTTCCTTTTGCATGTCACTGCT
NM_006062.2         TGTGTGCCCAATGCAGAGACCTCCTTTCCAGAAAACAACTTCCTTTTGCATGTCACTGCT
XM_005264082.1      TGTGTGCCCAATGCAGAGACCTCCTTTCCAGAAAACAACTTCCTTTTGCATGTCACTGCT
                    ************************************************************

HsCD00329698        CTGGAGGATATTAAGCCAGGAGAGGAAATTTGTATCAGCTACTTGGACTGCTGTCAGCGG
NM_006062.2         CTGGAGGATATTAAGCCAGGAGAGGAAATTTGTATCAGCTACTTGGACTGCTGTCAGCGG
XM_005264082.1      CTGGAGGATATTAAGCCAGGAGAGGAAATTTGTATCAGCTACTTGGACTGCTGTCAGCGG
                    ************************************************************

HsCD00329698        GAGCGCAGCCGCCACAGCCGCCACAAGATCCTCAGGGAGAACTATCTATTTGTCTGTTCC
NM_006062.2         GAGCGCAGCCGCCACAGCCGCCACAAGATCCTCAGGGAGAACTATCTATTTGTCTGTTCC
XM_005264082.1      GAGCGCAGCCGCCACAGCCGCCACAAGATCCTCAGGGAGAACTATCTATTTGTCTGTTCC
                    ************************************************************

HsCD00329698        TGTCCCAAATGCCTGGCAGAGGCTGATGAACCCAATGTGACCTCAGAAGAGGAAGAGGAA
NM_006062.2         TGTCCCAAATGCCTGGCAGAGGCTGATGAACCCAATGTGACCTCAGAAGAGGAAGAGGAA
XM_005264082.1      TGTCCCAAATGCCTGGCAGAGGCTGATGAACCCAATGTGACCTCAGAAGAGGAAGAGGAA
                    ************************************************************

HsCD00329698        GAGGAGGAGGAGGAGGAAGGAGAGCCAGAAGATGCAGAGCTGGGGGATGAGATGACTGAT
NM_006062.2         GAGGAGGAGGAGGAGGAAGGAGAGCCAGAAGATGCAGAGCTGGGGGATGAGATGACTGAT
XM_005264082.1      GAGGAGGAGGAGGAGGAAGGAGAGCCAGAAGATGCAGAGCTGGGGGATGAGATGACTGAT
                    ************************************************************

HsCD00329698        GTGTTG
NM_006062.2         GTGTGA
XM_005264082.1      GTGTGA
                    **** .

Clone

Cloning Information : 268155
HIP Master Clone ID : 193198
Original Clone ID : FLH268155.01L

Organism


TAX_ID : 9606
Species Specific ID: 10322

Genome

Chromosome : 2
Map Location : 2p13.2
Ensembl : ENSG00000135632