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KLHL12 (Homo sapiens) in pLenti6.2/V5-DEST (V5-tagged lentiviral mammalian expression vector)

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Explanation of Terms


Gene: Gene Symbol:  KLHL12
Gene Name:  kelch-like family member 12
Sequence             
Original Clone ID: FLH268162.01L
Keyword: None
Species: Homo sapiens
Type: cDNA
Vector Name: pLenti6.2/V5-DEST               Format:  FUSION
Source: HIP
Description: None
Comments: None
Mutation/
Discrepancy :
No / No
Publications: None
Authors:
HsCD00329704

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AVAILABLE
No restriction
Special MTA: None

 

Insert sequence: 1707nts         Open reading frame : 1 to 1707
Reference Sequence Alignment

ATGGGAGGCATTATGGCCCCCAAAGACATAATGACAAATACTCATGCTAAATCCATCCTC
AATTCAATGAACTCCCTCAGGAAGAGCAATACCCTCTGTGATGTGACATTGAGAGTAGAG
CAGAAAGACTTCCCTGCCCATCGGATTGTGCTGGCTGCCTGTAGTGATTACTTCTGTGCC
ATGTTCACTAGTGAGCTCTCAGAGAAGGGGAAACCTTATGTTGACATCCAAGGTTTGACT
GCCTCTACCATGGAAATTTTATTGGACTTTGTGTACACAGAAACAGTACATGTGACAGTG
GAGAATGTACAAGAACTGCTTCCTGCAGCCTGTCTGCTTCAGTTGAAAGGTGTGAAACAA
GCCTGCTGTGAGTTCTTAGAAAGTCAGTTGGACCCTTCTAATTGCCTGGGTATTAGGGAT
TTTGCTGAAACCCACAATTGTGTTGACCTGATGCAAGCAGCTGAGGTTTTTAGCCAGAAG
CATTTTCCTGAAGTGGTACAGCATGAAGAGTTCATTCTTCTGAGTCAAGGAGAGGTGGAA
AAGCTAATCAAGTGCGACGAAATTCAGGTGGATTCTGAAGAGCCAGTCTTTGAGGCTGTC
ATCAACTGGGTGAAGCATGCCAAGAAAGAGCGGGAAGAATCCTTGCCTAACCTGCTACAG
TATGTGCGGATGCCCCTACTAACCCCCAGGTATATCACAGATGTAATAGATGCTGAGCCT
TTCATCCGCTGTAGTTTACAATGCAGGGATCTGGTTGATGAAGCAAAGAAGTTTCATCTG
AGGCCTGAACTTCGGAGTCAGATGCAGGGACCCAGGACAAGGGCTCGCCTAGGAGCCAAT
GAAGTGCTTTTGGTGGTTGGGGGCTTTGGAAGCCAGCAGTCTCCCATTGATGTGGTAGAG
AAATATGACCCCAAGACTCAGGAGTGGAGCTTTTTGCCAAGCATCACTCGTAAGAGACGT
TATGTGGCCTCAGTGTCCCTTCATGACCGGATCTACGTCATTGGTGGCTATGATGGCCGT
TCCCGCCTTAGTTCAGTGGAATGTCTAGACTACACAGCAGATGAGGATGGGGTCTGGTAT
TCTGTGGCCCCTATGAATGTCCGACGAGGTCTTGCTGGAGCCACCACCCTGGGAGATATG
ATCTATGTCTCTGGAGGCTTTGATGGAAGCAGGCGTCACACCAGTATGGAGCGCTATGAT
CCAAACATTGACCAGTGGAGCATGCTGGGAGATATGCAGACAGCCCGGGAAGGTGCCGGA
CTCGTAGTGGCCAGTGGAGTGATCTACTGTCTAGGAGGATATGACGGCTTGAATATCTTA
AATTCAGTTGAGAAATACGACCCTCATACAGGACATTGGACTAATGTTACACCAATGGCC
ACCAAGCGTTCTGGTGCAGGAGTAGCCCTGCTGAATGACCATATTTATGTGGTGGGGGGA
TTTGATGGTACAGCCCACCTTTCTTCCGTTGAAGCATACAACATTCGCACTGATTCCTGG
ACAACTGTCACCAGTATGACCACTCCACGATGCTATGTAGGGGCCACAGTGCTTCGGGGG
AGACTCTATGCAATTGCAGGATATGATGGTAATTCCCTGCTAAGTAGCATTGAATGTTAT
GACCCTATCATCGACAGCTGGGAAGTCGTGACATCCATGGGAACCCAGCGCTGTGATGCT
GGTGTTTGTGTTCTCCGCGAGAAGTTG


Coding Sequence Details
Insert Sequence Verified?: N Verification Method: Insert was fully sequenced in parent vector
Reference Sequence Annotations:
End on reference sequence 1888
Start on reference sequence 182
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
5p GTACAAAAAAGCAGGCTCCACC(atg-goi)

3' Linker Sequence:
5p (goi-stop)GACCCAGCTTTCTTGTAC

 

 Recommended Growth Condition:

Distributed in bacterial strain : DH5-alpha T1 phage resistant
Antibiotic Selection: Host Type:  bacterial    Marker:  ampicillin
Host Type:  mammalian    Marker:  blasticidin
Bacterial Selection Condition:  100 ug/mL ampicillin
Growth Condition:  Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:  Commonly used conditions for ampicillin resistant plasmid clones.
Protein Expression Results: None
Recommended expression in: Not Applicable
       DyNA Vector Map

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Vector Name: pLenti6.2/V5-DEST

pLenti6.2V5-DEST in advanced viewed

Synonyms: pLenti6.2/V5-DEST
Sequencing Primer: Forward:  CMV forward
Reverse:  V5 reverse
Description: Lentiviral mammalian expression vector;CMV promoter and C-terminal V5 tag; ampicillin resistance in bacteria; blasticidin resistance in mammalian cells; Gateway recombinational cloning.
Comments: None
Size (bp): 8813
Parent Vector: None
Empty Vector: None
Properties: Gateway, acceptor (destination), lentiviral infection, mammalian expression, recombinational cloning, with tag/fusion/marker
Author Name: Invitrogen
Publications: None
Vector Map:         Vector Sequence:
Sequence:

 



Vector Features:

Type Name Description Start Position End Position
bacterial origin pUC ori pUC origin of replication 7733 8406
death cassette ccdB ccdB death cassette (removed when insert is present) 3767 3976
packaging signal HIV-1 psi HIV-1 psi packaging signal 521 565
poly-A signal SV40 SV40 polyadenylation signal 5639 5770
promoter RSV RSV/5' LTR hybrid promoter 1 410
promoter CMV CMV promoter driving gene of interest 1809 2392
promoter PGK PGK promoter to drive selection marker in mammalian cells 4266 4771
recombination site attR2 attR2 recombinational cloning site 4127 4142
recombination site attR1 attR1 recombinational cloning site 2440 2456
response element HIV-1 RRE HIV-1 Rev response element (RRE) 1075 1308
selectable marker AmpR Ampicillin resistance gene for selection in bacteria 6728 7588
selectable marker BlastR Blasticidin resistance gene in mammalian cells 4849 5249
selectable marker CmR chloramphenicol resistance marker (removed in plasmids with insert) 2673 3329
splice acceptor site 3' splice acceptor two 3' splice acceptors at 1656 and 1684 1656 1656
splice donor site 5' splice donor 5' splice donor 520 520
tag V5 C-terminal V5 epitope tag 4197 4238

 

  • Gene
  • Pathways
  • Protein
  • Clone
  • Organism
  • Genome
  • Reagents

Gene

 

Gene Symbol : KLHL12
Symbol Nomenclature : KLHL12
SYNONYM : C3IP1; DKIR; hDKIR
Designation : CUL3-interacting protein 1|DKIR homolog|kelch-like 12|kelch-like protein 12|kelch-like protein C3IP1
Full Nomenclature : kelch-like family member 12
GENEID : 59349
HGNC : 19360
MIM : 614522
Vega : OTTHUMG00000041385
Target GenBank: BC003183

 

Reference Sequence Alignment

  

HsCD00329704        ------------------------------------------------------------
NM_021633.2         ------------------------------------------------------------
XM_005245403.1      ATGGACGTGAATAAATTTGAGGCCTCTGTGGGTTTTCTCGATGTCAAAAAGTTCCTCAGT
                                                                                

HsCD00329704        ------------------------------------------------------ATGGGA
NM_021633.2         ------------------------------------------------------ATGGGA
XM_005245403.1      ACCTGGAAGTTGCAGAATCCAAGGACCCATTTTGTTCTTTCTCCGCACTGCTTTATGGGA
                                                                          ******

HsCD00329704        GGCATTATGGCCCCCAAAGACATAATGACAAATACTCATGCTAAATCCATCCTCAATTCA
NM_021633.2         GGCATTATGGCCCCCAAAGACATAATGACAAATACTCATGCTAAATCCATCCTCAATTCA
XM_005245403.1      GGCATTATGGCCCCCAAAGACATAATGACAAATACTCATGCTAAATCCATCCTCAATTCA
                    ************************************************************

HsCD00329704        ATGAACTCCCTCAGGAAGAGCAATACCCTCTGTGATGTGACATTGAGAGTAGAGCAGAAA
NM_021633.2         ATGAACTCCCTCAGGAAGAGCAATACCCTCTGTGATGTGACATTGAGAGTAGAGCAGAAA
XM_005245403.1      ATGAACTCCCTCAGGAAGAGCAATACCCTCTGTGATGTGACATTGAGAGTAGAGCAGAAA
                    ************************************************************

HsCD00329704        GACTTCCCTGCCCATCGGATTGTGCTGGCTGCCTGTAGTGATTACTTCTGTGCCATGTTC
NM_021633.2         GACTTCCCTGCCCATCGGATTGTGCTGGCTGCCTGTAGTGATTACTTCTGTGCCATGTTC
XM_005245403.1      GACTTCCCTGCCCATCGGATTGTGCTGGCTGCCTGTAGTGATTACTTCTGTGCCATGTTC
                    ************************************************************

HsCD00329704        ACTAGTGAGCTCTCAGAGAAGGGGAAACCTTATGTTGACATCCAAGGTTTGACTGCCTCT
NM_021633.2         ACTAGTGAGCTCTCAGAGAAGGGGAAACCTTATGTTGACATCCAAGGTTTGACTGCCTCT
XM_005245403.1      ACTAGTGAGCTCTCAGAGAAGGGGAAACCTTATGTTGACATCCAAGGTTTGACTGCCTCT
                    ************************************************************

HsCD00329704        ACCATGGAAATTTTATTGGACTTTGTGTACACAGAAACAGTACATGTGACAGTGGAGAAT
NM_021633.2         ACCATGGAAATTTTATTGGACTTTGTGTACACAGAAACGGTACATGTGACAGTGGAGAAT
XM_005245403.1      ACCATGGAAATTTTATTGGACTTTGTGTACACAGAAACAGTACATGTGACAGTGGAGAAT
                    **************************************.*********************

HsCD00329704        GTACAAGAACTGCTTCCTGCAGCCTGTCTGCTTCAGTTGAAAGGTGTGAAACAAGCCTGC
NM_021633.2         GTACAAGAACTGCTTCCTGCAGCCTGTCTGCTTCAGTTGAAAGGTGTGAAACAAGCCTGC
XM_005245403.1      GTACAAGAACTGCTTCCTGCAGCCTGTCTGCTTCAGTTGAAAGGTGTGAAACAAGCCTGC
                    ************************************************************

HsCD00329704        TGTGAGTTCTTAGAAAGTCAGTTGGACCCTTCTAATTGCCTGGGTATTAGGGATTTTGCT
NM_021633.2         TGTGAGTTCTTAGAAAGTCAGTTGGACCCTTCTAATTGCCTGGGTATTAGGGATTTTGCT
XM_005245403.1      TGTGAGTTCTTAGAAAGTCAGTTGGACCCTTCTAATTGCCTGGGTATTAGGGATTTTGCT
                    ************************************************************

HsCD00329704        GAAACCCACAATTGTGTTGACCTGATGCAAGCAGCTGAGGTTTTTAGCCAGAAGCATTTT
NM_021633.2         GAAACCCACAATTGTGTTGACCTGATGCAAGCAGCTGAGGTTTTTAGCCAGAAGCATTTT
XM_005245403.1      GAAACCCACAATTGTGTTGACCTGATGCAAGCAGCTGAGGTTTTTAGCCAGAAGCATTTT
                    ************************************************************

HsCD00329704        CCTGAAGTGGTACAGCATGAAGAGTTCATTCTTCTGAGTCAAGGAGAGGTGGAAAAGCTA
NM_021633.2         CCTGAAGTGGTACAGCATGAAGAGTTCATTCTTCTGAGTCAAGGAGAGGTGGAAAAGCTA
XM_005245403.1      CCTGAAGTGGTACAGCATGAAGAGTTCATTCTTCTGAGTCAAGGAGAGGTGGAAAAGCTA
                    ************************************************************

HsCD00329704        ATCAAGTGCGACGAAATTCAGGTGGATTCTGAAGAGCCAGTCTTTGAGGCTGTCATCAAC
NM_021633.2         ATCAAGTGCGACGAAATTCAGGTGGATTCTGAAGAGCCAGTCTTTGAGGCTGTCATCAAC
XM_005245403.1      ATCAAGTGCGACGAAATTCAGGTGGATTCTGAAGAGCCAGTCTTTGAGGCTGTCATCAAC
                    ************************************************************

HsCD00329704        TGGGTGAAGCATGCCAAGAAAGAGCGGGAAGAATCCTTGCCTAACCTGCTACAGTATGTG
NM_021633.2         TGGGTGAAGCATGCCAAGAAAGAGCGGGAAGAATCCTTGCCTAACCTGCTACAGTATGTG
XM_005245403.1      TGGGTGAAGCATGCCAAGAAAGAGCGGGAAGAATCCTTGCCTAACCTGCTACAGTATGTG
                    ************************************************************

HsCD00329704        CGGATGCCCCTACTAACCCCCAGGTATATCACAGATGTAATAGATGCTGAGCCTTTCATC
NM_021633.2         CGGATGCCCCTACTAACCCCCAGGTATATCACAGATGTAATAGATGCTGAGCCTTTCATC
XM_005245403.1      CGGATGCCCCTACTAACCCCCAGGTATATCACAGATGTAATAGATGCTGAGCCTTTCATC
                    ************************************************************

HsCD00329704        CGCTGTAGTTTACAATGCAGGGATCTGGTTGATGAAGCAAAGAAGTTTCATCTGAGGCCT
NM_021633.2         CGCTGTAGTTTACAATGCAGGGATCTGGTTGATGAAGCAAAGAAGTTTCATCTGAGGCCT
XM_005245403.1      CGCTGTAGTTTACAATGCAGGGATCTGGTTGATGAAGCAAAGAAGTTTCATCTGAGGCCT
                    ************************************************************

HsCD00329704        GAACTTCGGAGTCAGATGCAGGGACCCAGGACAAGGGCTCGCCTAGGAGCCAATGAAGTG
NM_021633.2         GAACTTCGGAGTCAGATGCAGGGACCCAGGACAAGGGCTCGCCTAGGAGCCAATGAAGTG
XM_005245403.1      GAACTTCGGAGTCAGATGCAGGGACCCAGGACAAGGGCTCGCCTAGGAGCCAATGAAGTG
                    ************************************************************

HsCD00329704        CTTTTGGTGGTTGGGGGCTTTGGAAGCCAGCAGTCTCCCATTGATGTGGTAGAGAAATAT
NM_021633.2         CTTTTGGTGGTTGGGGGCTTTGGAAGCCAGCAGTCTCCCATTGATGTGGTAGAGAAATAT
XM_005245403.1      CTTTTGGTGGTTGGGGGCTTTGGAAGCCAGCAGTCTCCCATTGATGTGGTAGAGAAATAT
                    ************************************************************

HsCD00329704        GACCCCAAGACTCAGGAGTGGAGCTTTTTGCCAAGCATCACTCGTAAGAGACGTTATGTG
NM_021633.2         GACCCCAAGACTCAGGAGTGGAGCTTTTTGCCAAGCATCACTCGTAAGAGACGTTATGTG
XM_005245403.1      GACCCCAAGACTCAGGAGTGGAGCTTTTTGCCAAGCATCACTCGTAAGAGACGTTATGTG
                    ************************************************************

HsCD00329704        GCCTCAGTGTCCCTTCATGACCGGATCTACGTCATTGGTGGCTATGATGGCCGTTCCCGC
NM_021633.2         GCCTCAGTGTCCCTTCATGACCGGATCTACGTCATTGGTGGCTATGATGGCCGTTCCCGC
XM_005245403.1      GCCTCAGTGTCCCTTCATGACCGGATCTACGTCATTGGTGGCTATGATGGCCGTTCCCGC
                    ************************************************************

HsCD00329704        CTTAGTTCAGTGGAATGTCTAGACTACACAGCAGATGAGGATGGGGTCTGGTATTCTGTG
NM_021633.2         CTTAGTTCAGTGGAATGTCTAGACTACACAGCAGATGAGGATGGGGTCTGGTATTCTGTG
XM_005245403.1      CTTAGTTCAGTGGAATGTCTAGACTACACAGCAGATGAGGATGGGGTCTGGTATTCTGTG
                    ************************************************************

HsCD00329704        GCCCCTATGAATGTCCGACGAGGTCTTGCTGGAGCCACCACCCTGGGAGATATGATCTAT
NM_021633.2         GCCCCTATGAATGTCCGACGAGGTCTTGCTGGAGCCACCACCCTGGGAGATATGATCTAT
XM_005245403.1      GCCCCTATGAATGTCCGACGAGGTCTTGCTGGAGCCACCACCCTGGGAGATATGATCTAT
                    ************************************************************

HsCD00329704        GTCTCTGGAGGCTTTGATGGAAGCAGGCGTCACACCAGTATGGAGCGCTATGATCCAAAC
NM_021633.2         GTCTCTGGAGGCTTTGATGGAAGCAGGCGTCACACCAGTATGGAGCGCTATGATCCAAAC
XM_005245403.1      GTCTCTGGAGGCTTTGATGGAAGCAGGCGTCACACCAGTATGGAGCGCTATGATCCAAAC
                    ************************************************************

HsCD00329704        ATTGACCAGTGGAGCATGCTGGGAGATATGCAGACAGCCCGGGAAGGTGCCGGACTCGTA
NM_021633.2         ATTGACCAGTGGAGCATGCTGGGGGATATGCAGACAGCCCGGGAAGGTGCCGGACTCGTA
XM_005245403.1      ATTGACCAGTGGAGCATGCTGGGAGATATGCAGACAGCCCGGGAAGGTGCCGGACTCGTA
                    ***********************.************************************

HsCD00329704        GTGGCCAGTGGAGTGATCTACTGTCTAGGAGGATATGACGGCTTGAATATCTTAAATTCA
NM_021633.2         GTGGCCAGTGGAGTGATCTACTGTCTAGGAGGATATGACGGCTTGAATATCTTAAATTCA
XM_005245403.1      GTGGCCAGTGGAGTGATCTACTGTCTAGGAGGATATGACGGCTTGAATATCTTAAATTCA
                    ************************************************************

HsCD00329704        GTTGAGAAATACGACCCTCATACAGGACATTGGACTAATGTTACACCAATGGCCACCAAG
NM_021633.2         GTTGAGAAATACGACCCTCATACAGGACATTGGACTAATGTTACACCAATGGCCACCAAG
XM_005245403.1      GTTGAGAAATACGACCCTCATACAGGACATTGGACTAATGTTACACCAATGGCCACCAAG
                    ************************************************************

HsCD00329704        CGTTCTGGTGCAGGAGTAGCCCTGCTGAATGACCATATTTATGTGGTGGGGGGATTTGAT
NM_021633.2         CGTTCTGGTGCAGGAGTAGCCCTGCTGAATGACCATATTTATGTGGTGGGGGGATTTGAT
XM_005245403.1      CGTTCTGGTGCAGGAGTAGCCCTGCTGAATGACCATATTTATGTGGTGGGGGGATTTGAT
                    ************************************************************

HsCD00329704        GGTACAGCCCACCTTTCTTCCGTTGAAGCATACAACATTCGCACTGATTCCTGGACAACT
NM_021633.2         GGTACAGCCCACCTTTCTTCCGTTGAAGCATACAACATTCGCACTGATTCCTGGACAACT
XM_005245403.1      GGTACAGCCCACCTTTCTTCCGTTGAAGCATACAACATTCGCACTGATTCCTGGACAACT
                    ************************************************************

HsCD00329704        GTCACCAGTATGACCACTCCACGATGCTATGTAGGGGCCACAGTGCTTCGGGGGAGACTC
NM_021633.2         GTCACCAGTATGACCACTCCACGATGCTATGTAGGGGCCACAGTGCTTCGGGGGAGACTC
XM_005245403.1      GTCACCAGTATGACCACTCCACGATGCTATGTAGGGGCCACAGTGCTTCGGGGGAGACTC
                    ************************************************************

HsCD00329704        TATGCAATTGCAGGATATGATGGTAATTCCCTGCTAAGTAGCATTGAATGTTATGACCCT
NM_021633.2         TATGCAATTGCAGGATATGATGGTAATTCCCTGCTAAGTAGCATTGAATGTTATGACCCT
XM_005245403.1      TATGCAATTGCAGGATATGATGGTAATTCCCTGCTAAGTAGCATTGAATGTTATGACCCT
                    ************************************************************

HsCD00329704        ATCATCGACAGCTGGGAAGTCGTGACATCCATGGGAACCCAGCGCTGTGATGCTGGTGTT
NM_021633.2         ATCATCGACAGCTGGGAAGTCGTGACATCCATGGGAACCCAGCGCTGTGATGCTGGTGTT
XM_005245403.1      ATCATCGACAGCTGGGAAGTCGTGACATCCATGGGAACCCAGCGCTGTGATGCTGGTGTT
                    ************************************************************

HsCD00329704        TGTGTTCTCCGCGAGAAGTTG
NM_021633.2         TGTGTTCTCCGCGAGAAGTGA
XM_005245403.1      TGTGTTCTCCGCGAGAAGTGA
                    ******************* .

Pathways

NCI : Canonical Wnt signaling pathway
Reactome : Signal Transduction
Reactome : Signaling by Wnt
Reactome : TCF dependent signaling in response to WNT
Reactome : degradation of DVL

Protein

SMART domain : BACK : BTB And C-terminal Kelch
SMART domain : BTB : Broad-Complex, Tramtrack and Bric a brac
SMART domain : Kelch :
UniProt : Q53G59
HPRD : 13925

Clone

Cloning Information : 268162
HIP Master Clone ID : 194795
Original Clone ID : FLH268162.01L

Organism


TAX_ID : 9606
Species Specific ID: 59349

Genome

Chromosome : 1
Map Location : 1q32.1
Ensembl : ENSG00000117153

Reagents

Labome : KLHL12-antibody