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GTF2B (Homo sapiens) in pLenti6.2/V5-DEST (V5-tagged lentiviral mammalian expression vector)

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Explanation of Terms


Gene: Gene Symbol:  GTF2B
Gene Name:  general transcription factor IIB
Sequence             
Original Clone ID: FLH268164.01L
Keyword: None
Species: Homo sapiens
Type: cDNA
Vector Name: pLenti6.2/V5-DEST               Format:  FUSION
Source: HIP
Description: None
Comments: None
Mutation/
Discrepancy :
No / No
Publications: None
Authors:
HsCD00329706

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AVAILABLE
No restriction
Special MTA: None

 

Insert sequence: 951nts         Open reading frame : 1 to 951
Reference Sequence Alignment

ATGGCGTCTACCAGCCGTTTGGATGCTCTTCCAAGAGTCACATGTCCAAACCATCCAGAT
GCGATTTTAGTGGAGGACTACAGAGCCGGTGATATGATCTGTCCTGAATGTGGCTTGGTT
GTAGGTGACCGGGTTATTGATGTGGGATCTGAATGGCGAACTTTCAGCAATGACAAAGCA
ACAAAAGATCCATCTCGAGTTGGAGATTCTCAGAATCCTCTTCTGAGTGATGGAGATTTG
TCTACCATGATTGGCAAGGGCACAGGAGCTGCAAGTTTTGACGAATTTGGCAATTCTAAG
TACCAGAATCGGAGAACAATGAGCAGTTCTGATCGGGCAATGATGAATGCATTCAAAGAA
ATCACTACCATGGCAGACAGAATCAATCTACCTCGAAATATAGTTGATCGAACAAATAAT
TTATTCAAGCAAGTATATGAACAGAAGAGCCTGAAGGGAAGAGCTAATGATGCTATAGCT
TCTGCTTGTCTCTATATTGCCTGTAGACAAGAAGGGGTTCCTAGGACATTTAAAGAAATA
TGTGCCGTATCACGAATTTCTAAGAAAGAAATTGGTCGGTGTTTTAAACTTATTTTGAAA
GCGCTAGAAACCAGTGTGGATTTGATTACAACTGGGGACTTCATGTCCAGGTTCTGTTCC
AACCTTTGTCTTCCTAAACAAGTACAGATGGCAGCTACACATATAGCCCGTAAAGCTGTG
GAATTGGACTTGGTTCCTGGGAGGAGCCCCATCTCTGTGGCAGCGGCAGCTATTTACATG
GCCTCACAGGCATCAGCTGAAAAGAGGACCCAAAAAGAAATTGGAGATATTGCTGGTGTT
GCTGATGTTACAATCAGACAGTCCTATAGACTGATCTATCCTCGAGCCCCAGATCTGTTT
CCTACAGACTTCAAATTTGACACCCCAGTGGACAAACTACCACAGCTATTG


Coding Sequence Details
Insert Sequence Verified?: N Verification Method: Insert was fully sequenced in parent vector
Reference Sequence Annotations:
End on reference sequence 1011
Start on reference sequence 61
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
5p GTACAAAAAAGCAGGCTCCACC(atg-goi)

3' Linker Sequence:
5p (goi-stop)GACCCAGCTTTCTTGTAC

 

 Recommended Growth Condition:

Distributed in bacterial strain : DH5-alpha T1 phage resistant
Antibiotic Selection: Host Type:  bacterial    Marker:  ampicillin
Host Type:  mammalian    Marker:  blasticidin
Bacterial Selection Condition:  100 ug/mL ampicillin
Growth Condition:  Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:  Commonly used conditions for ampicillin resistant plasmid clones.
Protein Expression Results: None
Recommended expression in: Not Applicable
       DyNA Vector Map

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Vector Name: pLenti6.2/V5-DEST

pLenti6.2V5-DEST in advanced viewed

Synonyms: pLenti6.2/V5-DEST
Sequencing Primer: Forward:  CMV forward
Reverse:  V5 reverse
Description: Lentiviral mammalian expression vector;CMV promoter and C-terminal V5 tag; ampicillin resistance in bacteria; blasticidin resistance in mammalian cells; Gateway recombinational cloning.
Comments: None
Size (bp): 8813
Parent Vector: None
Empty Vector: None
Properties: Gateway, acceptor (destination), lentiviral infection, mammalian expression, recombinational cloning, with tag/fusion/marker
Author Name: Invitrogen
Publications: None
Vector Map:         Vector Sequence:
Sequence:

 



Vector Features:

Type Name Description Start Position End Position
bacterial origin pUC ori pUC origin of replication 7733 8406
death cassette ccdB ccdB death cassette (removed when insert is present) 3767 3976
packaging signal HIV-1 psi HIV-1 psi packaging signal 521 565
poly-A signal SV40 SV40 polyadenylation signal 5639 5770
promoter RSV RSV/5' LTR hybrid promoter 1 410
promoter CMV CMV promoter driving gene of interest 1809 2392
promoter PGK PGK promoter to drive selection marker in mammalian cells 4266 4771
recombination site attR2 attR2 recombinational cloning site 4127 4142
recombination site attR1 attR1 recombinational cloning site 2440 2456
response element HIV-1 RRE HIV-1 Rev response element (RRE) 1075 1308
selectable marker AmpR Ampicillin resistance gene for selection in bacteria 6728 7588
selectable marker BlastR Blasticidin resistance gene in mammalian cells 4849 5249
selectable marker CmR chloramphenicol resistance marker (removed in plasmids with insert) 2673 3329
splice acceptor site 3' splice acceptor two 3' splice acceptors at 1656 and 1684 1656 1656
splice donor site 5' splice donor 5' splice donor 520 520
tag V5 C-terminal V5 epitope tag 4197 4238

 

  • Gene
  • Pathways
  • Protein
  • Clone
  • Organism
  • Genome
  • Reagents

Gene

 

Gene Symbol : GTF2B
Symbol Nomenclature : GTF2B
SYNONYM : TF2B; TFIIB
Designation : RNA polymerase II transcription factor IIB|S300-II|general transcription factor TFIIB|transcription initiation factor IIB
Full Nomenclature : general transcription factor IIB
GENEID : 2959
Locus Tag : RP11-82K18.1
HGNC : 4648
MIM : 189963
Vega : OTTHUMG00000010611
Target GenBank: BC021000

 

Reference Sequence Alignment

  

HsCD00329706      ATGGCGTCTACCAGCCGTTTGGATGCTCTTCCAAGAGTCACATGTCCAAACCATCCAGAT
NM_001514.5       ATGGCGTCTACCAGCCGTTTGGATGCTCTTCCAAGAGTCACATGTCCAAACCATCCAGAT
                  ************************************************************

HsCD00329706      GCGATTTTAGTGGAGGACTACAGAGCCGGTGATATGATCTGTCCTGAATGTGGCTTGGTT
NM_001514.5       GCGATTTTAGTGGAGGACTACAGAGCCGGTGATATGATCTGTCCTGAATGTGGCTTGGTT
                  ************************************************************

HsCD00329706      GTAGGTGACCGGGTTATTGATGTGGGATCTGAATGGCGAACTTTCAGCAATGACAAAGCA
NM_001514.5       GTAGGTGACCGGGTTATTGATGTGGGATCTGAATGGCGAACTTTCAGCAATGACAAAGCA
                  ************************************************************

HsCD00329706      ACAAAAGATCCATCTCGAGTTGGAGATTCTCAGAATCCTCTTCTGAGTGATGGAGATTTG
NM_001514.5       ACAAAAGATCCATCTCGAGTTGGAGATTCTCAGAATCCTCTTCTGAGTGATGGAGATTTG
                  ************************************************************

HsCD00329706      TCTACCATGATTGGCAAGGGCACAGGAGCTGCAAGTTTTGACGAATTTGGCAATTCTAAG
NM_001514.5       TCTACCATGATTGGCAAGGGCACAGGAGCTGCAAGTTTTGACGAATTTGGCAATTCTAAG
                  ************************************************************

HsCD00329706      TACCAGAATCGGAGAACAATGAGCAGTTCTGATCGGGCAATGATGAATGCATTCAAAGAA
NM_001514.5       TACCAGAATCGGAGAACAATGAGCAGTTCTGATCGGGCAATGATGAATGCATTCAAAGAA
                  ************************************************************

HsCD00329706      ATCACTACCATGGCAGACAGAATCAATCTACCTCGAAATATAGTTGATCGAACAAATAAT
NM_001514.5       ATCACTACCATGGCAGACAGAATCAATCTACCTCGAAATATAGTTGATCGAACAAATAAT
                  ************************************************************

HsCD00329706      TTATTCAAGCAAGTATATGAACAGAAGAGCCTGAAGGGAAGAGCTAATGATGCTATAGCT
NM_001514.5       TTATTCAAGCAAGTATATGAACAGAAGAGCCTGAAGGGAAGAGCTAATGATGCTATAGCT
                  ************************************************************

HsCD00329706      TCTGCTTGTCTCTATATTGCCTGTAGACAAGAAGGGGTTCCTAGGACATTTAAAGAAATA
NM_001514.5       TCTGCTTGTCTCTATATTGCCTGTAGACAAGAAGGGGTTCCTAGGACATTTAAAGAAATA
                  ************************************************************

HsCD00329706      TGTGCCGTATCACGAATTTCTAAGAAAGAAATTGGTCGGTGTTTTAAACTTATTTTGAAA
NM_001514.5       TGTGCCGTATCACGAATTTCTAAGAAAGAAATTGGTCGGTGTTTTAAACTTATTTTGAAA
                  ************************************************************

HsCD00329706      GCGCTAGAAACCAGTGTGGATTTGATTACAACTGGGGACTTCATGTCCAGGTTCTGTTCC
NM_001514.5       GCGCTAGAAACCAGTGTGGATTTGATTACAACTGGGGACTTCATGTCCAGGTTCTGTTCC
                  ************************************************************

HsCD00329706      AACCTTTGTCTTCCTAAACAAGTACAGATGGCAGCTACACATATAGCCCGTAAAGCTGTG
NM_001514.5       AACCTTTGTCTTCCTAAACAAGTACAGATGGCAGCTACACATATAGCCCGTAAAGCTGTG
                  ************************************************************

HsCD00329706      GAATTGGACTTGGTTCCTGGGAGGAGCCCCATCTCTGTGGCAGCGGCAGCTATTTACATG
NM_001514.5       GAATTGGACTTGGTTCCTGGGAGGAGCCCCATCTCTGTGGCAGCGGCAGCTATTTACATG
                  ************************************************************

HsCD00329706      GCCTCACAGGCATCAGCTGAAAAGAGGACCCAAAAAGAAATTGGAGATATTGCTGGTGTT
NM_001514.5       GCCTCACAGGCATCAGCTGAAAAGAGGACCCAAAAAGAAATTGGAGATATTGCTGGTGTT
                  ************************************************************

HsCD00329706      GCTGATGTTACAATCAGACAGTCCTATAGACTGATCTATCCTCGAGCCCCAGATCTGTTT
NM_001514.5       GCTGATGTTACAATCAGACAGTCCTATAGACTGATCTATCCTCGAGCCCCAGATCTGTTT
                  ************************************************************

HsCD00329706      CCTACAGACTTCAAATTTGACACCCCAGTGGACAAACTACCACAGCTATTG
NM_001514.5       CCTACAGACTTCAAATTTGACACCCCAGTGGACAAACTACCACAGCTATAA
                  *************************************************:.

Pathways

Panther : General transcription regulation
Panther : Transcription regulation by bZIP transcription factor
Reactome : Disease
Reactome : Gene Expression
Reactome : HIV Infection
Reactome : HIV Life Cycle
Reactome : HIV Transcription Initiation
Reactome : Late Phase of HIV Life Cycle
Reactome : RNA Polymerase II HIV Promoter Escape
Reactome : RNA Polymerase II Pre-transcription Events
Reactome : RNA Polymerase II Promoter Escape
Reactome : RNA Polymerase II Transcription
Reactome : RNA Polymerase II Transcription Initiation
Reactome : RNA Polymerase II Transcription Initiation And Promoter Clearance
Reactome : RNA Polymerase II Transcription Pre-Initiation And Promoter Opening
Reactome : Transcription
Reactome : Transcription of the HIV genome
WikiPathway : Eukaryotic Transcription Initiation

Clone

Cloning Information : 268164
HIP Master Clone ID : 194798
Original Clone ID : FLH268164.01L

Organism


TAX_ID : 9606
Species Specific ID: 2959

Genome

Chromosome : 1
Map Location : 1p22-p21
Ensembl : ENSG00000137947

Reagents

Labome : TFIIB-antibody