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LARP (Homo sapiens) in pLenti6.2/V5-DEST (V5-tagged lentiviral mammalian expression vector)

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Explanation of Terms


Gene: Gene Symbol:  LARP1
Gene Name:  La ribonucleoprotein domain family, member 1
Sequence             
Original Clone ID: FLH268441.01L
Keyword: None
Species: Homo sapiens
Type: cDNA
Vector Name: pLenti6.2/V5-DEST               Format:  FUSION
Source: HIP
Description: None
Comments: None
Mutation/
Discrepancy :
No / No
Publications: None
Authors:
HsCD00329708

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AVAILABLE
No restriction
Special MTA: None

 

Insert sequence: 558nts         Open reading frame : 1 to 558
Reference Sequence Alignment

ATGAACACACTCTTCCGCTTCTGGTCCTTCTTCCTCCGAGATCACTTCAACAAAAAGATG
TATGAGGAGTTCAAGCAGCTGGCTCTGGAGGACGCCAAAGAAGGCTACAGATATGGTTTG
GAGTGCCTTTTTCGATACTACAGTTATGGCCTGGAAAAGAAGTTCCGGCTGGACATATTC
AAGGATTTTCAGGAGGAAACGGTGAAGGACTATGAAGCTGGCCAACTGTATGGGCTGGAG
AAGTTCTGGGCCTTCTTGAAATATTCCAAAGCCAAAAATTTGGACATTGACCCCAAACTG
CAAGAATACCTCGGCAAATTCCGACGTCTTGAAGACTTCCGAGTAGATCCCCCCATGGGT
GAGGAGGGCAACCACAAGCGACACTCAGTGGTAGCAGGAGGTGGCGGCGGTGAGGGCAGG
AAGCGGTGCCCCTCCCAGTCTTCCAGCAGGCCTGCTGCCATGATCAGCCAACCCCCTACA
CCACCCACCGGCCAGCCTGTCCGGGAAGATGCCAAATGGACAAGCCAGCACTCGAACACA
CAGACTTTGGGAAAGTTG


Coding Sequence Details
Insert Sequence Verified?: N Verification Method: Insert was fully sequenced in parent vector
Reference Sequence Annotations:
End on reference sequence 3093
Start on reference sequence 2536
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
5p GTACAAAAAAGCAGGCTCCACC(atg-goi)

3' Linker Sequence:
5p (goi-stop)GACCCAGCTTTCTTGTAC

 

 Recommended Growth Condition:

Distributed in bacterial strain : DH5-alpha T1 phage resistant
Antibiotic Selection: Host Type:  bacterial    Marker:  ampicillin
Host Type:  mammalian    Marker:  blasticidin
Bacterial Selection Condition:  100 ug/mL ampicillin
Growth Condition:  Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:  Commonly used conditions for ampicillin resistant plasmid clones.
Protein Expression Results: None
Recommended expression in: Not Applicable
       DyNA Vector Map

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Vector Name: pLenti6.2/V5-DEST

pLenti6.2V5-DEST in advanced viewed

Synonyms: pLenti6.2/V5-DEST
Sequencing Primer: Forward:  CMV forward
Reverse:  V5 reverse
Description: Lentiviral mammalian expression vector;CMV promoter and C-terminal V5 tag; ampicillin resistance in bacteria; blasticidin resistance in mammalian cells; Gateway recombinational cloning.
Comments: None
Size (bp): 8813
Parent Vector: None
Empty Vector: None
Properties: Gateway, acceptor (destination), lentiviral infection, mammalian expression, recombinational cloning, with tag/fusion/marker
Author Name: Invitrogen
Publications: None
Vector Map:         Vector Sequence:
Sequence:

 



Vector Features:

Type Name Description Start Position End Position
bacterial origin pUC ori pUC origin of replication 7733 8406
death cassette ccdB ccdB death cassette (removed when insert is present) 3767 3976
packaging signal HIV-1 psi HIV-1 psi packaging signal 521 565
poly-A signal SV40 SV40 polyadenylation signal 5639 5770
promoter RSV RSV/5' LTR hybrid promoter 1 410
promoter CMV CMV promoter driving gene of interest 1809 2392
promoter PGK PGK promoter to drive selection marker in mammalian cells 4266 4771
recombination site attR2 attR2 recombinational cloning site 4127 4142
recombination site attR1 attR1 recombinational cloning site 2440 2456
response element HIV-1 RRE HIV-1 Rev response element (RRE) 1075 1308
selectable marker AmpR Ampicillin resistance gene for selection in bacteria 6728 7588
selectable marker BlastR Blasticidin resistance gene in mammalian cells 4849 5249
selectable marker CmR chloramphenicol resistance marker (removed in plasmids with insert) 2673 3329
splice acceptor site 3' splice acceptor two 3' splice acceptors at 1656 and 1684 1656 1656
splice donor site 5' splice donor 5' splice donor 520 520
tag V5 C-terminal V5 epitope tag 4197 4238

 

  • Gene
  • Protein
  • Clone
  • Organism
  • Genome
  • Reagents

Gene

 

Gene Symbol : LARP1
Symbol Nomenclature : LARP1
SYNONYM : LARP
Designation : la-related protein 1
Full Nomenclature : La ribonucleoprotein domain family, member 1
GENEID : 23367
HGNC : 29531
MIM : 612059
Vega : OTTHUMG00000130191
Target GenBank: BC001460

 

Reference Sequence Alignment

  

HsCD00329708      ------------------------------------------------------------
NM_015315.4       ATGCTTTGGAGGGTGCTTTTGTCAAAGAGGCCTCCTTTCCCTCACCCAGAGCTGGATTTC
                                                                              

HsCD00329708      ------------------------------------------------------------
NM_015315.4       CAAGAGGCTCCCATACCTAGCTGCCCTGGCAGACTCCCAGGGAGGAAAAACAGCGTGGCC
                                                                              

HsCD00329708      ------------------------------------------------------------
NM_015315.4       TTGGCAGCTGCCCCGAGGAAGGAGCCCACAGGTGACAGGGAGAAGCCATTGCCATTCCCT
                                                                              

HsCD00329708      ------------------------------------------------------------
NM_015315.4       GTCCTGGCCCCCTTCAGCAACCCTGAACACTCTGCTCCAGCCAAGGTGGTGAGGGCAGCT
                                                                              

HsCD00329708      ------------------------------------------------------------
NM_015315.4       GTTCCTAAACAGCGCAAAGGCAGCAAGGTTGGTGACTTTGGAGATGCAATCAATTGGCCC
                                                                              

HsCD00329708      ------------------------------------------------------------
NM_015315.4       ACACCTGGAGAGATAGCCCACAAGAGTGTTCAGCCACAGTCCCACAAGCCTCAGCCTACC
                                                                              

HsCD00329708      ------------------------------------------------------------
NM_015315.4       CGTAAACTGCCACCCAAGAAGGACATGAAGGAACAGGAGAAAGGAGAAGGGAGTGATAGT
                                                                              

HsCD00329708      ------------------------------------------------------------
NM_015315.4       AAGGAGAGTCCAAAAACCAAATCAGATGAATCAGGGGAGGAAAAGAATGGAGATGAGGAT
                                                                              

HsCD00329708      ------------------------------------------------------------
NM_015315.4       TGCCAGCGAGGCGGGCAGAAGAAGAAAGGAAACAAACACAAGTGGGTTCCATTACAAATA
                                                                              

HsCD00329708      ------------------------------------------------------------
NM_015315.4       GACATGAAGCCTGAAGTGCCCAGAGAGAAACTGGCTTCACGCCCCACTCGCCCACCGGAG
                                                                              

HsCD00329708      ------------------------------------------------------------
NM_015315.4       CCTAGACACATACCTGCCAATCGCGGAGAGATCAAAGGGTCTGAGTCTGCCACCTACGTG
                                                                              

HsCD00329708      ------------------------------------------------------------
NM_015315.4       CCCGTGGCCCCCCCCACCCCAGCCTGGCAACCAGAGATCAAACCGGAGCCTGCCTGGCAC
                                                                              

HsCD00329708      ------------------------------------------------------------
NM_015315.4       GACCAGGATGAGACATCGAGTGTGAAGAGTGATGGGGCTGGTGGGGCGCGGGCTTCCTTC
                                                                              

HsCD00329708      ------------------------------------------------------------
NM_015315.4       CGTGGCCGTGGACGGGGGCGTGGTCGCGGCCGGGGACGCGGCCGGGGTGGCACTCGAACC
                                                                              

HsCD00329708      ------------------------------------------------------------
NM_015315.4       CATTTTGACTACCAGTTTGGCTACCGAAAGTTTGATGGTGTGGAGGGGCCTCGTACGCCC
                                                                              

HsCD00329708      ------------------------------------------------------------
NM_015315.4       AAGTACATGAACAACATCACCTACTACTTTGACAATGTCAGCAGCACCGAGCTTTACAGT
                                                                              

HsCD00329708      ------------------------------------------------------------
NM_015315.4       GTGGATCAGGAACTGCTCAAAGACTACATCAAGCGCCAGATTGAATACTACTTCAGCGTG
                                                                              

HsCD00329708      ------------------------------------------------------------
NM_015315.4       GACAATTTAGAGCGAGACTTCTTCCTGCGAAGGAAAATGGATGCTGATGGTTTCCTACCC
                                                                              

HsCD00329708      ------------------------------------------------------------
NM_015315.4       ATCACCCTTATTGCTTCCTTCCACCGAGTGCAGGCCCTTACCACTGACATTTCACTCATC
                                                                              

HsCD00329708      ------------------------------------------------------------
NM_015315.4       TTTGCGGCCCTAAAGGACAGCAAGGTGGTGGAGATCGTTGATGAGAAAGTTCGTAGGAGG
                                                                              

HsCD00329708      ------------------------------------------------------------
NM_015315.4       GAGGAACCAGAAAAGTGGCCTCTTCCCCCAATAGTGGATTATTCACAGACTGATTTCTCC
                                                                              

HsCD00329708      ------------------------------------------------------------
NM_015315.4       CAGCTTCTCAACTGCCCTGAATTTGTTCCCCGTCAGCACTACCAAAAGGAGACAGAGTCG
                                                                              

HsCD00329708      ------------------------------------------------------------
NM_015315.4       GCACCTGGCTCTCCTCGTGCAGTCACCCCAGTGCCAACCAAAACAGAGGAGGTCAGCAAC
                                                                              

HsCD00329708      ------------------------------------------------------------
NM_015315.4       CTAAAGACACTACCCAAGGGCCTGTCTGCCAGCCTGCCTGACCTGGATTCTGAGAACTGG
                                                                              

HsCD00329708      ------------------------------------------------------------
NM_015315.4       ATTGAAGTGAAGAAGAGGCCTCGGCCATCCCCAGCACGGCCCAAGAAGTCAGAGGAGTCC
                                                                              

HsCD00329708      ------------------------------------------------------------
NM_015315.4       AGATTTTCCCACCTGACCTCTCTGCCTCAGCAGCTGCCTTCCCAGCAGCTGATGTCCAAG
                                                                              

HsCD00329708      ------------------------------------------------------------
NM_015315.4       GATCAGGATGAGCAAGAGGAACTGGATTTTCTGTTTGACGAGGAGATGGAGCAGATGGAT
                                                                              

HsCD00329708      ------------------------------------------------------------
NM_015315.4       GGGCGGAAGAACACCTTCACTGCCTGGTCTGATGAGGAATCTGACTATGAGATTGATGAC
                                                                              

HsCD00329708      ------------------------------------------------------------
NM_015315.4       AGGGATGTCAACAAGATCCTCATTGTCACCCAGACACCACATTACATGCGCCGGCACCCA
                                                                              

HsCD00329708      ------------------------------------------------------------
NM_015315.4       GGGGGGGACCGCACAGGCAACCACACCTCGCGTGCCAAGATGAGCGCCGAACTGGCCAAG
                                                                              

HsCD00329708      ------------------------------------------------------------
NM_015315.4       GTCATTAATGATGGCCTCTTCTACTATGAGCAGGACCTGTGGGCTGAAAAGTTTGAACCT
                                                                              

HsCD00329708      ------------------------------------------------------------
NM_015315.4       GAGTATTCCCAGATCAAGCAAGAAGTCGAGAACTTCAAAAAGGTCAATATGATCAGCCGG
                                                                              

HsCD00329708      ------------------------------------------------------------
NM_015315.4       GAGCAGTTTGACACACTGACCCCTGAGCCCCCTGTGGATCCCAACCAGGAAGTTCCTCCT
                                                                              

HsCD00329708      ------------------------------------------------------------
NM_015315.4       GGGCCACCTCGGTTCCAGCAAGTTCCTACGGATGCCCTGGCCAACAAGTTGTTTGGTGCT
                                                                              

HsCD00329708      ------------------------------------------------------------
NM_015315.4       CCTGAGCCCTCCACCATCGCCCGCTCTCTACCAACCACTGTCCCAGAGTCACCAAACTAC
                                                                              

HsCD00329708      ------------------------------------------------------------
NM_015315.4       CGCAACACCAGGACCCCTCGCACTCCCCGGACACCACAGCTCAAAGACTCAAGCCAGACA
                                                                              

HsCD00329708      ------------------------------------------------------------
NM_015315.4       TCACGGTTTTACCCAGTGGTGAAAGAAGGACGGACACTGGATGCCAAGATGCCTCGAAAA
                                                                              

HsCD00329708      ------------------------------------------------------------
NM_015315.4       AGAAAGACAAGACACAGTTCAAACCCACCCTTGGAGAGCCATGTGGGCTGGGTGATGGAT
                                                                              

HsCD00329708      ------------------------------------------------------------
NM_015315.4       TCCCGTGAGCACAGGCCCCGTACTGCTTCCATCAGCTCCAGCCCCTCAGAAGGGACGCCT
                                                                              

HsCD00329708      ------------------------------------------------------------
NM_015315.4       ACAGTTGGCAGCTATGGCTGTACCCCTCAGTCATTGCCCAAGTTCCAGCATCCTTCCCAT
                                                                              

HsCD00329708      ------------------------------------------------------------
NM_015315.4       GAACTGCTCAAGGAAAATGGCTTCACACAACACGTCTACCATAAGTATCGTAGGCGCTGC
                                                                              

HsCD00329708      ------------------------------------------ATGAACACACTCTTCCGC
NM_015315.4       CTTAATGAGCGGAAACGCTTGGGCATTGGCCAGTCTCAGGAGATGAACACACTCTTCCGC
                                                            ******************

HsCD00329708      TTCTGGTCCTTCTTCCTCCGAGATCACTTCAACAAAAAGATGTATGAGGAGTTCAAGCAG
NM_015315.4       TTCTGGTCCTTCTTCCTCCGAGATCACTTCAACAAAAAGATGTATGAGGAGTTCAAGCAG
                  ************************************************************

HsCD00329708      CTGGCTCTGGAGGACGCCAAAGAAGGCTACAGATATGGTTTGGAGTGCCTTTTTCGATAC
NM_015315.4       CTGGCTCTGGAGGACGCCAAAGAAGGCTACAGATATGGTTTGGAGTGCCTTTTTCGATAC
                  ************************************************************

HsCD00329708      TACAGTTATGGCCTGGAAAAGAAGTTCCGGCTGGACATATTCAAGGATTTTCAGGAGGAA
NM_015315.4       TACAGTTATGGCCTGGAAAAGAAGTTCCGGCTGGACATATTCAAGGATTTTCAGGAGGAA
                  ************************************************************

HsCD00329708      ACGGTGAAGGACTATGAAGCTGGCCAACTGTATGGGCTGGAGAAGTTCTGGGCCTTCTTG
NM_015315.4       ACGGTGAAGGACTATGAAGCTGGCCAACTGTATGGGCTGGAGAAGTTCTGGGCCTTCTTG
                  ************************************************************

HsCD00329708      AAATATTCCAAAGCCAAAAATTTGGACATTGACCCCAAACTGCAAGAATACCTCGGCAAA
NM_015315.4       AAATATTCCAAAGCCAAAAATTTGGACATTGACCCCAAACTGCAAGAATACCTCGGCAAA
                  ************************************************************

HsCD00329708      TTCCGACGTCTTGAAGACTTCCGAGTAGATCCCCCCATGGGTGAGGAGGGCAACCACAAG
NM_015315.4       TTCCGACGTCTTGAAGACTTCCGAGTAGATCCCCCCATGGGTGAGGAGGGCAACCACAAG
                  ************************************************************

HsCD00329708      CGACACTCAGTGGTAGCAGGAGGTGGCGGCGGTGAGGGCAGGAAGCGGTGCCCCTCCCAG
NM_015315.4       CGACACTCAGTGGTAGCAGGAGGTGGCGGCGGTGAGGGCAGGAAGCGGTGCCCCTCCCAG
                  ************************************************************

HsCD00329708      TCTTCCAGCAGGCCTGCTGCCATGATCAGCCAACCCCCTACACCACCCACCGGCCAGCCT
NM_015315.4       TCTTCCAGCAGGCCTGCTGCCATGATCAGCCAACCCCCTACACCACCCACCGGCCAGCCT
                  ************************************************************

HsCD00329708      GTCCGGGAAGATGCCAAATGGACAAGCCAGCACTCGAACACACAGACTTTGGGAAAGTTG
NM_015315.4       GTCCGGGAAGATGCCAAATGGACAAGCCAGCACTCGAACACACAGACTTTGGGAAAGTGA
                  ********************************************************** .

Clone

Cloning Information : 268441
HIP Master Clone ID : 193456
Original Clone ID : FLH268441.01L

Organism


TAX_ID : 9606
Species Specific ID: 23367

Genome

Chromosome : 5
Map Location : 5q33.2
Ensembl : ENSG00000155506

Reagents

Labome : LARP1-antibody