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MKRN3 (Homo sapiens) in pLenti6.2/V5-DEST (V5-tagged lentiviral mammalian expression vector)

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Explanation of Terms


Gene: Gene Symbol:  MKRN3
Gene Name:  makorin ring finger protein 3
Sequence             
Original Clone ID: FLH268451.01L
Keyword: None
Species: Homo sapiens
Type: cDNA
Vector Name: pLenti6.2/V5-DEST               Format:  FUSION
Source: HIP
Description: None
Comments: None
Mutation/
Discrepancy :
No / No
Publications: None
Authors:
HsCD00329718

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AVAILABLE
No restriction
Special MTA: None

 

Insert sequence: 1524nts         Open reading frame : 1 to 1524
Reference Sequence Alignment

ATGGAAGAGCCTGCAGCTCCCTCAGAAGCCCACGAGGCAGCCGGGGCCCAGGCAGGTGCT
GAGGCAGCAAGGGAGGGTGTGTCTGGGCCGGACCTTCCCGTCTGTGAGCCCTCCGGGGAA
TCTGCTGCTCCAGATTCAGCCCTGCCACATGCGGCAAGGGGCTGGGCCCCCTTCCCTGTA
GCTCCAGTCCCTGCCCACCTCCGCAGAGGAGGCCTGAGGCCTGCCCCAGCCTCAGGAGGA
GGAGCCTGGCCCAGTCCGTTGCCAAGCCGAAGCAGCGGCATTTGGACAAAGCAGATCATC
TGCAGGTATTATATACATGGGCAGTGCAAGGAGGGGGAGAACTGTCGCTATTCGCACGAC
CTTTCTGGTCGGAAGATGGCCACTGAGGGTGGCGTTTCGCCGCCTGGGGCCTCTGCAGGT
GGAGGCCCTAGCACGGCTGCGCACATCGAGCCCCCGACTCAGGAAGTGGCGGAAGCCCCC
CCGGCTGCATCCTCCCTTTCCTTGCCTGTGATTGGCTCGGCTGCTGAAAGGGGTTTCTTT
GAAGCCGAGAGAGACAATGCAGACCGTGGAGCTGCTGGAGGAGCAGGTGTAGAAAGCTGG
GCGGATGCCATTGAGTTTGTTCCAGGGCAGCCCTACCGGGGCCGCTGGGTTGCATCTGCC
CCCGAGGCTCCTCTACAGAGCTCAGAGACTGAGAGGAAGCAGATGGCTGTGGGCAGTGGG
TTGCGGTTTTGCTATTATGCTTCCAGGGGAGTTTGCTTTCGTGGGGAGAGCTGTATGTAC
CTCCATGGAGACATATGCGACATGTGTGGGCTGCAGACCTTGCACCCCATGGATGCTGCC
CAGAGGGAAGAACATATGAGGGCCTGCATTGAAGCACACGAGAAAGATATGGAACTCTCG
TTTGCTGTGCAGCGTGGTATGGACAAGGTGTGTGGCATCTGCATGGAGGTTGTCTATGAG
AAGGCCAACCCCAATGACCGCCGCTTTGGCATTCTTTCCAATTGCAACCATTCCTTCTGT
ATTAGGTGTATCCGCAGGTGGAGAAGTGCCAGACAGTTTGAGAACAGGATCGTCAAGTCT
TGCCCACAGTGCAGGGTCACCTCTGAATTGGTCATTCCCAGTGAGTTCTGGGTGGAGGAG
GAGGAAGAGAAGCAGAAACTTATTCAGCAATACAAGGAGGCAATGAGCAACAAGGCCTGC
AGGTATTTTGCGGAAGGCAGGGGTAACTGCCCATTTGGAGACACATGCTTTTACAAGCAT
GAATACCCTGAGGGCTGGGGAGATGAGCCTCCTGGGCCAGGTGGTGGGTCATTCAGCGCA
TACTGGCATCAACTTGTGGAGCCTGTGCGAATGGGAGAGGGCAACATGCTCTATAAAAGC
ATTAAGAAGGAGCTTGTCGTGCTTCGGCTGGCCAGTCTGTTGTTTAAGCGGTTTCTTTCA
CTGAGAGATGAGTTACCCTTCTCTGAGGACCAGTGGGACTTGCTTCATTATGAGCTGGAA
GAATATTTCAATTTGATTCTGTTG


Coding Sequence Details
Insert Sequence Verified?: N Verification Method: Insert was fully sequenced in parent vector
Reference Sequence Annotations:
End on reference sequence 1623
Start on reference sequence 100
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
5p GTACAAAAAAGCAGGCTCCACC(atg-goi)

3' Linker Sequence:
5p (goi-stop)GACCCAGCTTTCTTGTAC

 

 Recommended Growth Condition:

Distributed in bacterial strain : DH5-alpha T1 phage resistant
Antibiotic Selection: Host Type:  bacterial    Marker:  ampicillin
Host Type:  mammalian    Marker:  blasticidin
Bacterial Selection Condition:  100 ug/mL ampicillin
Growth Condition:  Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:  Commonly used conditions for ampicillin resistant plasmid clones.
Protein Expression Results: None
Recommended expression in: Not Applicable
       DyNA Vector Map

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Vector Name: pLenti6.2/V5-DEST

pLenti6.2V5-DEST in advanced viewed

Synonyms: pLenti6.2/V5-DEST
Sequencing Primer: Forward:  CMV forward
Reverse:  V5 reverse
Description: Lentiviral mammalian expression vector;CMV promoter and C-terminal V5 tag; ampicillin resistance in bacteria; blasticidin resistance in mammalian cells; Gateway recombinational cloning.
Comments: None
Size (bp): 8813
Parent Vector: None
Empty Vector: None
Properties: Gateway, acceptor (destination), lentiviral infection, mammalian expression, recombinational cloning, with tag/fusion/marker
Author Name: Invitrogen
Publications: None
Vector Map:         Vector Sequence:
Sequence:

 



Vector Features:

Type Name Description Start Position End Position
bacterial origin pUC ori pUC origin of replication 7733 8406
death cassette ccdB ccdB death cassette (removed when insert is present) 3767 3976
packaging signal HIV-1 psi HIV-1 psi packaging signal 521 565
poly-A signal SV40 SV40 polyadenylation signal 5639 5770
promoter RSV RSV/5' LTR hybrid promoter 1 410
promoter CMV CMV promoter driving gene of interest 1809 2392
promoter PGK PGK promoter to drive selection marker in mammalian cells 4266 4771
recombination site attR2 attR2 recombinational cloning site 4127 4142
recombination site attR1 attR1 recombinational cloning site 2440 2456
response element HIV-1 RRE HIV-1 Rev response element (RRE) 1075 1308
selectable marker AmpR Ampicillin resistance gene for selection in bacteria 6728 7588
selectable marker BlastR Blasticidin resistance gene in mammalian cells 4849 5249
selectable marker CmR chloramphenicol resistance marker (removed in plasmids with insert) 2673 3329
splice acceptor site 3' splice acceptor two 3' splice acceptors at 1656 and 1684 1656 1656
splice donor site 5' splice donor 5' splice donor 520 520
tag V5 C-terminal V5 epitope tag 4197 4238

 

  • Gene
  • Protein
  • Clone
  • Organism
  • Genome
  • Reagents

Gene

 

Gene Symbol : MKRN3
Symbol Nomenclature : MKRN3
SYNONYM : CPPB2; D15S9; RNF63; ZFP127; ZNF127
Designation : RING finger protein 63|probable E3 ubiquitin-protein ligase makorin-3|zinc finger protein 127
Full Nomenclature : makorin ring finger protein 3
GENEID : 7681
HGNC : 7114
MIM : 603856
Vega : OTTHUMG00000129160
Target GenBank: BC044639

 

Reference Sequence Alignment

  

HsCD00329718      ATGGAAGAGCCTGCAGCTCCCTCAGAAGCCCACGAGGCAGCCGGGGCCCAGGCAGGTGCT
NM_005664.3       ATGGAAGAGCCTGCAGCTCCCTCAGAAGCCCACGAGGCAGCCGGGGCCCAGGCAGGTGCT
                  ************************************************************

HsCD00329718      GAGGCAGCAAGGGAGGGTGTGTCTGGGCCGGACCTTCCCGTCTGTGAGCCCTCCGGGGAA
NM_005664.3       GAGGCAGCAAGGGAGGGTGTGTCTGGGCCGGACCTTCCCGTCTGTGAGCCCTCCGGGGAA
                  ************************************************************

HsCD00329718      TCTGCTGCTCCAGATTCAGCCCTGCCACATGCGGCAAGGGGCTGGGCCCCCTTCCCTGTA
NM_005664.3       TCTGCTGCTCCAGATTCAGCCCTGCCACATGCGGCAAGGGGCTGGGCCCCCTTCCCTGTA
                  ************************************************************

HsCD00329718      GCTCCAGTCCCTGCCCACCTCCGCAGAGGAGGCCTGAGGCCTGCCCCAGCCTCAGGAGGA
NM_005664.3       GCTCCAGTCCCTGCCCACCTCCGCAGAGGAGGCCTGAGGCCTGCCCCAGCCTCAGGAGGA
                  ************************************************************

HsCD00329718      GGAGCCTGGCCCAGTCCGTTGCCAAGCCGAAGCAGCGGCATTTGGACAAAGCAGATCATC
NM_005664.3       GGAGCCTGGCCCAGTCCGTTGCCAAGCCGAAGCAGCGGCATTTGGACAAAGCAGATCATC
                  ************************************************************

HsCD00329718      TGCAGGTATTATATACATGGGCAGTGCAAGGAGGGGGAGAACTGTCGCTATTCGCACGAC
NM_005664.3       TGCAGGTATTATATACATGGGCAGTGCAAGGAGGGGGAGAACTGTCGCTATTCGCACGAC
                  ************************************************************

HsCD00329718      CTTTCTGGTCGGAAGATGGCCACTGAGGGTGGCGTTTCGCCGCCTGGGGCCTCTGCAGGT
NM_005664.3       CTTTCTGGTCGGAAGATGGCCACTGAGGGTGGCGTTTCGCCGCCTGGGGCCTCTGCAGGT
                  ************************************************************

HsCD00329718      GGAGGCCCTAGCACGGCTGCGCACATCGAGCCCCCGACTCAGGAAGTGGCGGAAGCCCCC
NM_005664.3       GGAGGCCCTAGCACGGCTGCGCACATCGAGCCCCCGACTCAGGAAGTGGCGGAAGCCCCC
                  ************************************************************

HsCD00329718      CCGGCTGCATCCTCCCTTTCCTTGCCTGTGATTGGCTCGGCTGCTGAAAGGGGTTTCTTT
NM_005664.3       CCGGCTGCATCCTCCCTTTCCTTGCCTGTGATTGGCTCGGCTGCTGAAAGGGGTTTCTTT
                  ************************************************************

HsCD00329718      GAAGCCGAGAGAGACAATGCAGACCGTGGAGCTGCTGGAGGAGCAGGTGTAGAAAGCTGG
NM_005664.3       GAAGCCGAGAGAGACAATGCAGACCGTGGAGCTGCTGGAGGAGCAGGTGTAGAAAGCTGG
                  ************************************************************

HsCD00329718      GCGGATGCCATTGAGTTTGTTCCAGGGCAGCCCTACCGGGGCCGCTGGGTTGCATCTGCC
NM_005664.3       GCGGATGCCATTGAGTTTGTTCCAGGGCAGCCCTACCGGGGCCGCTGGGTTGCATCTGCC
                  ************************************************************

HsCD00329718      CCCGAGGCTCCTCTACAGAGCTCAGAGACTGAGAGGAAGCAGATGGCTGTGGGCAGTGGG
NM_005664.3       CCCGAGGCTCCTCTACAGAGCTCAGAGACTGAGAGGAAGCAGATGGCTGTGGGCAGTGGG
                  ************************************************************

HsCD00329718      TTGCGGTTTTGCTATTATGCTTCCAGGGGAGTTTGCTTTCGTGGGGAGAGCTGTATGTAC
NM_005664.3       TTGCGGTTTTGCTATTATGCTTCCAGGGGAGTTTGCTTTCGTGGGGAGAGCTGTATGTAC
                  ************************************************************

HsCD00329718      CTCCATGGAGACATATGCGACATGTGTGGGCTGCAGACCTTGCACCCCATGGATGCTGCC
NM_005664.3       CTCCATGGAGACATATGCGACATGTGTGGGCTGCAGACCTTGCACCCCATGGATGCTGCC
                  ************************************************************

HsCD00329718      CAGAGGGAAGAACATATGAGGGCCTGCATTGAAGCACACGAGAAAGATATGGAACTCTCG
NM_005664.3       CAGAGGGAAGAACATATGAGGGCCTGCATTGAAGCACACGAGAAAGATATGGAACTCTCG
                  ************************************************************

HsCD00329718      TTTGCTGTGCAGCGTGGTATGGACAAGGTGTGTGGCATCTGCATGGAGGTTGTCTATGAG
NM_005664.3       TTTGCTGTGCAGCGTGGTATGGACAAGGTGTGTGGCATCTGCATGGAGGTTGTCTATGAG
                  ************************************************************

HsCD00329718      AAGGCCAACCCCAATGACCGCCGCTTTGGCATTCTTTCCAATTGCAACCATTCCTTCTGT
NM_005664.3       AAGGCCAACCCCAATGACCGCCGCTTTGGCATTCTTTCCAATTGCAACCATTCCTTCTGT
                  ************************************************************

HsCD00329718      ATTAGGTGTATCCGCAGGTGGAGAAGTGCCAGACAGTTTGAGAACAGGATCGTCAAGTCT
NM_005664.3       ATTAGGTGTATCCGCAGGTGGAGAAGTGCCAGACAGTTTGAGAACAGGATCGTCAAGTCT
                  ************************************************************

HsCD00329718      TGCCCACAGTGCAGGGTCACCTCTGAATTGGTCATTCCCAGTGAGTTCTGGGTGGAGGAG
NM_005664.3       TGCCCACAGTGCAGGGTCACCTCTGAATTGGTCATTCCCAGTGAGTTCTGGGTGGAGGAG
                  ************************************************************

HsCD00329718      GAGGAAGAGAAGCAGAAACTTATTCAGCAATACAAGGAGGCAATGAGCAACAAGGCCTGC
NM_005664.3       GAGGAAGAGAAGCAGAAACTTATTCAGCAATACAAGGAGGCAATGAGCAACAAGGCCTGC
                  ************************************************************

HsCD00329718      AGGTATTTTGCGGAAGGCAGGGGTAACTGCCCATTTGGAGACACATGCTTTTACAAGCAT
NM_005664.3       AGGTATTTTGCGGAAGGCAGGGGTAACTGCCCATTTGGAGACACATGCTTTTACAAGCAT
                  ************************************************************

HsCD00329718      GAATACCCTGAGGGCTGGGGAGATGAGCCTCCTGGGCCAGGTGGTGGGTCATTCAGCGCA
NM_005664.3       GAATACCCTGAGGGCTGGGGAGATGAGCCTCCTGGGCCAGGTGGTGGGTCATTCAGCGCA
                  ************************************************************

HsCD00329718      TACTGGCATCAACTTGTGGAGCCTGTGCGAATGGGAGAGGGCAACATGCTCTATAAAAGC
NM_005664.3       TACTGGCATCAACTTGTGGAGCCTGTGCGAATGGGAGAGGGCAACATGCTCTATAAAAGC
                  ************************************************************

HsCD00329718      ATTAAGAAGGAGCTTGTCGTGCTTCGGCTGGCCAGTCTGTTGTTTAAGCGGTTTCTTTCA
NM_005664.3       ATTAAGAAGGAGCTTGTCGTGCTTCGGCTGGCCAGTCTGTTGTTTAAGCGGTTTCTTTCA
                  ************************************************************

HsCD00329718      CTGAGAGATGAGTTACCCTTCTCTGAGGACCAGTGGGACTTGCTTCATTATGAGCTGGAA
NM_005664.3       CTGAGAGATGAGTTACCCTTCTCTGAGGACCAGTGGGACTTGCTTCATTATGAGCTGGAA
                  ************************************************************

HsCD00329718      GAATATTTCAATTTGATTCTGTTG
NM_005664.3       GAATATTTCAATTTGATTCTGTAG
                  **********************:*

Protein

SMART domain : ZnF_C3H1 : zinc finger
SMART domain : RING : Ring finger
UniProt : Q13064
HPRD : 04836

Clone

Cloning Information : 268451
HIP Master Clone ID : 193470
Original Clone ID : FLH268451.01L

Organism


TAX_ID : 9606
Species Specific ID: 7681

Genome

Chromosome : 15
Map Location : 15q11-q13
Ensembl : ENSG00000179455

Reagents

Labome : MKRN3-antibody