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ZBTB2 (Homo sapiens) in pLenti6.2/V5-DEST (V5-tagged lentiviral mammalian expression vector)

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Explanation of Terms


Gene: Gene Symbol:  ZBTB2
Gene Name:  zinc finger and BTB domain containing 2
Sequence             
Original Clone ID: FLH268454.01L
Keyword: None
Species: Homo sapiens
Type: cDNA
Vector Name: pLenti6.2/V5-DEST               Format:  FUSION
Source: HIP
Description: None
Comments: None
Mutation/
Discrepancy :
No / No
Publications: None
Authors:
HsCD00329721

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AVAILABLE
No restriction
Special MTA: None

 

Insert sequence: 1545nts         Open reading frame : 1 to 1545
Reference Sequence Alignment

ATGGATTTGGCCAACCATGGACTTATTCTACTGCAACAGTTAAACGCTCAGCGAGAGTTT
GGTTTCCTGTGTGACTGCACGGTTGCAATCGGCGATGTATACTTCAAGGCACACAAATCA
GTTCTTGCTTCATTCTCCAATTACTTTAAGATGTTGTTTGTCCATCAGACCAGTGAGTGT
GTCCGCTTGAAACCAACTGACATACAGCCCGACATCTTCAGCTATCTCTTACACTTGATG
TACACTGGGAAGATGGCGCCTCAGCTCATCGACCCGGTTCGATTAGAACAGGGCATCAAG
TTTCTGCACGCCTACCCGCTCATTCAGGAAGCCAGCCTCGCCAGCCAGGGAGCCTTTTCT
CACCCTGACCAAGTTTTCCCACTGGCTTCTTCATTGTATGGCATTCAGATTGCAGATCAT
CAGTTGAGACAAGCCACCAAGATTGCTTCAGCACCTGAAAAACTCGGGCGAGATCCACGG
CCACAGACCTCCAGGATAAGCCAGGAGCAGGTCCCTGAGGCCTCACAGCTCTCCCAGCTG
ACTTCAAATCTGGCCCAGGTGAATCGGACAAATATGACTCCCTCAGACCCCCTGCAGACC
TCGCTGTCTCCAGAACTTGTTTCCACTCCTGTTCCTCCCCCTCCTCCCGGGGAGGAGACC
AATCTGGAAGCATCTTCCTCCGATGAGCAGCCTGCGTCCCTCACAATAGCCCACGTCAAG
CCAAGCATCATGAAGAGGAATGGGAGCTTTCCAAAGTACTATGCCTGCCACCTGTGTGGA
CGGCGCTTCACTCTCCGGAGCAGCTTACGTGAACACCTCCAGATCCACACAGGAGTACCT
TTCACATCTAGCCAACAGGGAGAAAGTCGCGTCCCCCTGACTCTCTGTAGCAATGCAGCT
GACCTCGGGAAAGATGCCATGGAAGTGCCTGAAGCCGGGATGATAAGTGACAGTGAGCTG
CAGCACATCTCTGATTCTCCCATCATCGATGGGCAGCAGCAGTCGGAAACCCCACCCCCC
TCAGACATTGCTGACATTGACAACCTGGAGCAGGCCGACCAGGAGAGGGAGGTGAAGAGG
CGGAAGTACGAGTGCACAATATGTGGACGCAAATTTATCCAGAAAAGCCACTGGAGGGAA
CACATGTACATACACACCGGGAAGCCTTTCAAGTGCAGCACTTGTGACAAAAGCTTTTGC
AGGGCCAACCAGGCTGCCCGCCACGTGTGCCTCAACCAGAGCATCGACACTTACACCATG
GTGGACAAACAGACTCTGGAACTCTGCACATTTGAGGAAGGGAGTCAGATGGACAACATG
CTGGTGCAAACAAACAAACCCTACAAATGCAACTTGTGTGACAAAACATTCTCCACTCCC
AATGAGGTTGTTAAACATTCATGCCAAAACCAGAACTCGGATGTTTTTGCCCTAGACGAA
GGGCGATCCATTCTCCTGGGCAGTGGGGACTCGGAAGTAACGGAGCCTGACCACCCAGTG
TTAGCTTCCATCAAAAAGGAACAAGAAACCGTCTTACTAGACTTG


Coding Sequence Details
Insert Sequence Verified?: N Verification Method: Insert was fully sequenced in parent vector
Reference Sequence Annotations:
End on reference sequence 1680
Start on reference sequence 136
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
5p GTACAAAAAAGCAGGCTCCACC(atg-goi)

3' Linker Sequence:
5p (goi-stop)GACCCAGCTTTCTTGTAC

 

 Recommended Growth Condition:

Distributed in bacterial strain : DH5-alpha T1 phage resistant
Antibiotic Selection: Host Type:  bacterial    Marker:  ampicillin
Host Type:  mammalian    Marker:  blasticidin
Bacterial Selection Condition:  100 ug/mL ampicillin
Growth Condition:  Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:  Commonly used conditions for ampicillin resistant plasmid clones.
Protein Expression Results: None
Recommended expression in: Not Applicable
       DyNA Vector Map

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Vector Name: pLenti6.2/V5-DEST

pLenti6.2V5-DEST in advanced viewed

Synonyms: pLenti6.2/V5-DEST
Sequencing Primer: Forward:  CMV forward
Reverse:  V5 reverse
Description: Lentiviral mammalian expression vector;CMV promoter and C-terminal V5 tag; ampicillin resistance in bacteria; blasticidin resistance in mammalian cells; Gateway recombinational cloning.
Comments: None
Size (bp): 8813
Parent Vector: None
Empty Vector: None
Properties: Gateway, acceptor (destination), lentiviral infection, mammalian expression, recombinational cloning, with tag/fusion/marker
Author Name: Invitrogen
Publications: None
Vector Map:         Vector Sequence:
Sequence:

 



Vector Features:

Type Name Description Start Position End Position
bacterial origin pUC ori pUC origin of replication 7733 8406
death cassette ccdB ccdB death cassette (removed when insert is present) 3767 3976
packaging signal HIV-1 psi HIV-1 psi packaging signal 521 565
poly-A signal SV40 SV40 polyadenylation signal 5639 5770
promoter RSV RSV/5' LTR hybrid promoter 1 410
promoter CMV CMV promoter driving gene of interest 1809 2392
promoter PGK PGK promoter to drive selection marker in mammalian cells 4266 4771
recombination site attR2 attR2 recombinational cloning site 4127 4142
recombination site attR1 attR1 recombinational cloning site 2440 2456
response element HIV-1 RRE HIV-1 Rev response element (RRE) 1075 1308
selectable marker AmpR Ampicillin resistance gene for selection in bacteria 6728 7588
selectable marker BlastR Blasticidin resistance gene in mammalian cells 4849 5249
selectable marker CmR chloramphenicol resistance marker (removed in plasmids with insert) 2673 3329
splice acceptor site 3' splice acceptor two 3' splice acceptors at 1656 and 1684 1656 1656
splice donor site 5' splice donor 5' splice donor 520 520
tag V5 C-terminal V5 epitope tag 4197 4238

 

  • Gene
  • Protein
  • Clone
  • Organism
  • Genome
  • Reagents

Gene

 

Gene Symbol : ZBTB2
Symbol Nomenclature : ZBTB2
SYNONYM : ZNF437
Designation : zinc finger and BTB domain-containing protein 2
Full Nomenclature : zinc finger and BTB domain containing 2
GENEID : 57621
HGNC : 20868
Vega : OTTHUMG00000015834
Target GenBank: BC020172

 

Reference Sequence Alignment

  

HsCD00329721        ATGGATTTGGCCAACCATGGACTTATTCTACTGCAACAGTTAAACGCTCAGCGAGAGTTT
XM_005267076.1      ATGGATTTGGCCAACCATGGACTTATTCTACTGCAACAGTTAAACGCTCAGCGAGAGTTT
NM_020861.1         ATGGATTTGGCCAACCATGGACTTATTCTACTGCAACAGTTAAACGCTCAGCGAGAGTTT
                    ************************************************************

HsCD00329721        GGTTTCCTGTGTGACTGCACGGTTGCAATCGGCGATGTATACTTCAAGGCACACAAATCA
XM_005267076.1      GGTTTCCTGTGTGACTGCACGGTTGCAATCGGCGATGTATACTTCAAGGCACACAAATCA
NM_020861.1         GGTTTCCTGTGTGACTGCACGGTTGCAATCGGCGATGTATACTTCAAGGCACACAAATCA
                    ************************************************************

HsCD00329721        GTTCTTGCTTCATTCTCCAATTACTTTAAGATGTTGTTTGTCCATCAGACCAGTGAGTGT
XM_005267076.1      GTTCTTGCTTCATTCTCCAATTACTTTAAGATGTTGTTTGTCCATCAGACCAGTGAGTGT
NM_020861.1         GTTCTTGCTTCATTCTCCAATTACTTTAAGATGTTGTTTGTCCATCAGACCAGTGAGTGT
                    ************************************************************

HsCD00329721        GTCCGCTTGAAACCAACTGACATACAGCCCGACATCTTCAGCTATCTCTTACACTTGATG
XM_005267076.1      GTCCGCTTGAAACCAACTGACATACAGCCCGACATCTTCAGCTATCTCTTACACTTGATG
NM_020861.1         GTCCGCTTGAAACCAACTGACATACAGCCCGACATCTTCAGCTATCTCTTACACTTGATG
                    ************************************************************

HsCD00329721        TACACTGGGAAGATGGCGCCTCAGCTCATCGACCCGGTTCGATTAGAACAGGGCATCAAG
XM_005267076.1      TACACTGGGAAGATGGCGCCTCAGCTCATCGACCCGGTTCGATTAGAACAGGGCATCAAG
NM_020861.1         TACACTGGGAAGATGGCGCCTCAGCTCATCGACCCGGTTCGATTAGAACAGGGCATCAAG
                    ************************************************************

HsCD00329721        TTTCTGCACGCCTACCCGCTCATTCAGGAAGCCAGCCTCGCCAGCCAGGGAGCCTTTTCT
XM_005267076.1      TTTCTGCACGCCTACCCGCTCATTCAGGAAGCCAGCCTCGCCAGCCAGGGAGCCTTTTCT
NM_020861.1         TTTCTGCACGCCTACCCGCTCATTCAGGAAGCCAGCCTCGCCAGCCAGGGAGCCTTTTCT
                    ************************************************************

HsCD00329721        CACCCTGACCAAGTTTTCCCACTGGCTTCTTCATTGTATGGCATTCAGATTGCAGATCAT
XM_005267076.1      CACCCTGACCAAGTTTTCCCACTGGCTTCTTCATTGTATGGCATTCAGATTGCAGATCAT
NM_020861.1         CACCCTGACCAAGTTTTCCCACTGGCTTCTTCATTGTATGGCATTCAGATTGCAGATCAT
                    ************************************************************

HsCD00329721        CAGTTGAGACAAGCCACCAAGATTGCTTCAGCACCTGAAAAACTCGGGCGAGATCCACGG
XM_005267076.1      CAGTTGAGACAAGCCACCAAGATTGCTTCAGCACCTGAAAAACTCGGGCGAGATCCACGG
NM_020861.1         CAGTTGAGACAAGCCACCAAGATTGCTTCAGCACCTGAAAAACTCGGGCGAGATCCACGG
                    ************************************************************

HsCD00329721        CCACAGACCTCCAGGATAAGCCAGGAGCAGGTCCCTGAGGCCTCACAGCTCTCCCAGCTG
XM_005267076.1      CCACAGACCTCCAGGATAAGCCAGGAGCAGGTCCCTGAGGCCTCACAGCTCTCCCAGCTG
NM_020861.1         CCACAGACCTCCAGGATAAGCCAGGAGCAGGTCCCTGAGGCCTCACAGCTCTCCCAGCTG
                    ************************************************************

HsCD00329721        ACTTCAAATCTGGCCCAGGTGAATCGGACAAATATGACTCCCTCAGACCCCCTGCAGACC
XM_005267076.1      ACTTCAAATCTGGCCCAGGTGAATCGGACAAATATGACTCCCTCAGACCCCCTGCAGACC
NM_020861.1         ACTTCAAATCTGGCCCAGGTGAATCGGACAAATATGACTCCCTCAGACCCCCTGCAGACC
                    ************************************************************

HsCD00329721        TCGCTGTCTCCAGAACTTGTTTCCACTCCTGTTCCTCCCCCTCCTCCCGGGGAGGAGACC
XM_005267076.1      TCGCTGTCTCCAGAACTTGTTTCCACTCCTGTTCCTCCCCCTCCTCCCGGGGAGGAGACC
NM_020861.1         TCGCTGTCTCCAGAACTTGTTTCCACTCCTGTTCCTCCCCCTCCTCCCGGGGAGGAGACC
                    ************************************************************

HsCD00329721        AATCTGGAAGCATCTTCCTCCGATGAGCAGCCTGCGTCCCTCACAATAGCCCACGTCAAG
XM_005267076.1      AATCTGGAAGCATCTTCCTCCGATGAGCAGCCTGCGTCCCTCACAATAGCCCACGTCAAG
NM_020861.1         AATCTGGAAGCATCTTCCTCCGATGAGCAGCCTGCGTCCCTCACAATAGCCCACGTCAAG
                    ************************************************************

HsCD00329721        CCAAGCATCATGAAGAGGAATGGGAGCTTTCCAAAGTACTATGCCTGCCACCTGTGTGGA
XM_005267076.1      CCAAGCATCATGAAGAGGAATGGGAGCTTTCCAAAGTACTATGCCTGCCACCTGTGTGGA
NM_020861.1         CCAAGCATCATGAAGAGGAATGGGAGCTTTCCAAAGTACTATGCCTGCCACCTGTGTGGA
                    ************************************************************

HsCD00329721        CGGCGCTTCACTCTCCGGAGCAGCTTACGTGAACACCTCCAGATCCACACAGGAGTACCT
XM_005267076.1      CGGCGCTTCACTCTCCGGAGCAGCTTACGTGAACACCTCCAGATCCACACAGGAGTACCT
NM_020861.1         CGGCGCTTCACTCTCCGGAGCAGCTTACGTGAACACCTCCAGATCCACACAGGAGTACCT
                    ************************************************************

HsCD00329721        TTCACATCTAGCCAACAGGGAGAAAGTCGCGTCCCCCTGACTCTCTGTAGCAATGCAGCT
XM_005267076.1      TTCACATCTAGCCAACAGGGAGAAAGTCGCGTCCCCCTGACTCTCTGTAGCAATGCAGCT
NM_020861.1         TTCACATCTAGCCAACAGGGAGAAAGTCGCGTCCCCCTGACTCTCTGTAGCAATGCAGCT
                    ************************************************************

HsCD00329721        GACCTCGGGAAAGATGCCATGGAAGTGCCTGAAGCCGGGATGATAAGTGACAGTGAGCTG
XM_005267076.1      GACCTCGGGAAAGATGCCATGGAAGTGCCTGAAGCCGGGATGATAAGTGACAGTGAGCTG
NM_020861.1         GACCTCGGGAAAGATGCCATGGAAGTGCCTGAAGCCGGGATGATAAGTGACAGTGAGCTG
                    ************************************************************

HsCD00329721        CAGCACATCTCTGATTCTCCCATCATCGATGGGCAGCAGCAGTCGGAAACCCCACCCCCC
XM_005267076.1      CAGCACATCTCTGATTCTCCCATCATCGATGGGCAGCAGCAGTCGGAAACCCCACCCCCC
NM_020861.1         CAGCACATCTCTGATTCTCCCATCATCGATGGGCAGCAGCAGTCGGAAACCCCACCCCCC
                    ************************************************************

HsCD00329721        TCAGACATTGCTGACATTGACAACCTGGAGCAGGCCGACCAGGAGAGGGAGGTGAAGAGG
XM_005267076.1      TCAGACATTGCTGACATTGACAACCTGGAGCAGGCCGACCAGGAGAGGGAGGTGAAGAGG
NM_020861.1         TCAGACATTGCTGACATTGACAACCTGGAGCAGGCCGACCAGGAGAGGGAGGTGAAGAGG
                    ************************************************************

HsCD00329721        CGGAAGTACGAGTGCACAATATGTGGACGCAAATTTATCCAGAAAAGCCACTGGAGGGAA
XM_005267076.1      CGGAAGTACGAGTGCACAATATGTGGACGCAAATTTATCCAGAAAAGCCACTGGAGGGAA
NM_020861.1         CGGAAGTACGAGTGCACAATATGTGGACGCAAATTTATCCAGAAAAGCCACTGGAGGGAA
                    ************************************************************

HsCD00329721        CACATGTACATACACACCGGGAAGCCTTTCAAGTGCAGCACTTGTGACAAAAGCTTTTGC
XM_005267076.1      CACATGTACATACACACCGGGAAGCCTTTCAAGTGCAGCACTTGTGACAAAAGCTTTTGC
NM_020861.1         CACATGTACATACACACCGGGAAGCCTTTCAAGTGCAGCACTTGTGACAAAAGCTTTTGC
                    ************************************************************

HsCD00329721        AGGGCCAACCAGGCTGCCCGCCACGTGTGCCTCAACCAGAGCATCGACACTTACACCATG
XM_005267076.1      AGGGCCAACCAGGCTGCCCGCCACGTGTGCCTCAACCAGAGCATCGACACTTACACCATG
NM_020861.1         AGGGCCAACCAGGCTGCCCGCCACGTGTGCCTCAACCAGAGCATCGACACTTACACCATG
                    ************************************************************

HsCD00329721        GTGGACAAACAGACTCTGGAACTCTGCACATTTGAGGAAGGGAGTCAGATGGACAACATG
XM_005267076.1      GTGGACAAACAGACTCTGGAACTCTGCACATTTGAGGAAGGGAGTCAGATGGACAACATG
NM_020861.1         GTGGACAAACAGACTCTGGAACTCTGCACATTTGAGGAAGGGAGTCAGATGGACAACATG
                    ************************************************************

HsCD00329721        CTGGTGCAAACAAACAAACCCTACAAATGCAACTTGTGTGACAAAACATTCTCCACTCCC
XM_005267076.1      CTGGTGCAAACAAACAAACCCTACAAATGCAACTTGTGTGACAAAACATTCTCCACTCCC
NM_020861.1         CTGGTGCAAACAAACAAACCCTACAAATGCAACTTGTGTGACAAAACATTCTCCACTCCC
                    ************************************************************

HsCD00329721        AATGAGGTTGTTAAACATTCATGCCAAAACCAGAACTCGGATGTTTTTGCCCTAGACGAA
XM_005267076.1      AATGAGGTTGTTAAACATTCATGCCAAAACCAGAACTCGGATGTTTTTGCCCTAGACGAA
NM_020861.1         AATGAGGTTGTTAAACATTCATGCCAAAACCAGAACTCGGATGTTTTTGCCCTAGACGAA
                    ************************************************************

HsCD00329721        GGGCGATCCATTCTCCTGGGCAGTGGGGACTCGGAAGTAACGGAGCCTGACCACCCAGTG
XM_005267076.1      GGGCGATCCATTCTCCTGGGCAGTGGGGACTCGGAAGTAACGGAGCCTGACCACCCAGTG
NM_020861.1         GGGCGATCCATTCTCCTGGGCAGTGGGGACTCGGAAGTAACGGAGCCTGACCACCCAGTG
                    ************************************************************

HsCD00329721        TTAGCTTCCATCAAAAAGGAACAAGAAACCGTCTTACTAGACTTG
XM_005267076.1      TTAGCTTCCATCAAAAAGGAACAAGAAACCGTCTTACTAGACTGA
NM_020861.1         TTAGCTTCCATCAAAAAGGAACAAGAAACCGTCTTACTAGACTGA
                    ******************************************* .

Protein

SMART domain : BTB : Broad-Complex, Tramtrack and Bric a brac
SMART domain : ZnF_C2H2 : zinc finger
UniProt : Q8N680
HPRD : 10314

Clone

Cloning Information : 268454
HIP Master Clone ID : 193476
Original Clone ID : FLH268454.01L

Organism


TAX_ID : 9606
Species Specific ID: 57621

Genome

Chromosome : 6
Map Location : 6q25.1
Ensembl : ENSG00000181472

Reagents

Labome : ZBTB2-antibody