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ZNF394 (Homo sapiens) in pLenti6.2/V5-DEST (V5-tagged lentiviral mammalian expression vector)

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Explanation of Terms


Gene: Gene Symbol:  ZNF394
Gene Name:  zinc finger protein 394
Sequence             
Original Clone ID: FLH268459.01L
Keyword: None
Species: Homo sapiens
Type: cDNA
Vector Name: pLenti6.2/V5-DEST               Format:  FUSION
Source: HIP
Description: None
Comments: None
Mutation/
Discrepancy :
No / No
Publications: None
Authors:
HsCD00329725

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AVAILABLE
No restriction
Special MTA: None

 

Insert sequence: 1686nts         Open reading frame : 1 to 1686
Reference Sequence Alignment

ATGAATTCCAGCTTGACCGCCCAGAGGCGCGGCAGTGACGCCGAGTTGGGACCCTGGGTG
ATGGCTGCGAGGTCCAAGGACGCGGCGCCGTCCCAACGCGACGGACTTTTGCCCGTGAAA
GTGGAGGAAGACTCACCCGGAAGTTGGGAGCCCAACTATCCCGCGGCTTCGCCGGACCCC
GAAACTTCTCGACTGCACTTTAGGCAGCTGCGTTACCAGGAGGTGGCTGGACCGGAAGAG
GCGCTGAGCCGGCTCCGAGAACTCTGTCGTCGGTGGCTGAGACCCGAGCTGCTCTCCAAG
GAGCAGATCCTGGAGCTGCTGGTGCTGGAGCAGTTCCTCACCATCCTGCCCGAGGAGCTT
CAAGCCTGGGTGCGAGAGCACTGCCCAGAGAGCGGGGAGGAGGCGGTGGCCGTGGTGCGG
GCTCTGCAGCGAGCGCTCGATGGAACCTCATCCCAGGGGATGGTGACTTTCGAGGACACG
GCTGTGTCTCTAACCTGGGAGGAGTGGGAGCGCCTGGACCCAGCACGGAGGGACTTCTGC
AGAGAGAGTGCGCAGAAGGATTCCGGGAGCACAGTTCCGCCGAGTTTGGAAAGCAGAGTG
GAGAACAAAGAGTTGATTCCAATGCAACAAATTTTAGAAGAAGCGGAGCCACAGGGGCAA
CTACAAGAAGCGTTCCAGGGGAAGCGCCCCCTGTTTTCTAAGTGTGGCAGTACCCATGAG
GACAGGGTGGAAAAGCAGTCCGGAGACCCCTTGCCCCTGAAACTTGAAAATTCTCCTGAA
GCAGAAGGACTCAACAGCATCTCAGATGTCAATAAGAATGGTTCCATAGAAGGGGAAGAC
TCTAAAAATAATGAATTGCAGAACAGTGCCAGGTGTTCCAACCTTGTTCTATGTCAGCAC
ATCCCGAAAGCAGAGAGGCCCACTGACAGTGAGGAACACGGGAACAAGTGCAAGCAAAGT
TTCCACATGGTGACGTGGCACGTGCTGAAACCTCACAAGTCTGACAGTGGAGACAGTTTC
CATCATTCCAGCCTTTTTGAGACCCAGAGGCAGCTCCATGAAGAAAGACCTTATAAATGT
GGTAACTGTGGGAAGAGTTTCAAACAACGCTCTGACCTCTTTAGACACCAGAGAATCCAC
ACAGGTGAGAAACCCTATGGCTGCCAAGAATGTGGGAAAAGCTTCAGCCAGAGTGCTGCC
CTGACCAAGCACCAGAGGACACACACAGGCGAGAAGCCGTACACCTGTCTGAAATGTGGG
GAGCGCTTCAGGCAGAATTCACACCTAAATCGTCATCAAAGTACCCACAGTAGAGACAAA
CATTTTAAATGTGAGGAATGCGGGGAAACCTGTCATATTTCCAACCTTTTTAGACATCAG
AGACTACATAAAGGGGAAAGACCCTATAAGTGTGAAGAATGCGAGAAGAGCTTCAAACAG
CGCTCTGACCTCTTTAAACACCACAGAATCCACACTGGGGAGAAGCCCTATGGATGTTCC
GTCTGTGGGAAACGCTTCAATCAGAGTGCAACCCTCATTAAACACCAGAGAATTCACACT
GGGGAAAAGCCTTACAAATGTCTTGAATGTGGGGAAAGATTTAGACAAAGTACACACCTT
ATCCGACACCAAAGAATTCATCAAAATAAAGTGCTGTCGGCTGGGCGTGGTGGCTCACGC
CTATTG


Coding Sequence Details
Insert Sequence Verified?: N Verification Method: Insert was fully sequenced in parent vector
Reference Sequence Annotations:
End on reference sequence 1847
Start on reference sequence 162
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
5p GTACAAAAAAGCAGGCTCCACC(atg-goi)

3' Linker Sequence:
5p (goi-stop)GACCCAGCTTTCTTGTAC

 

 Recommended Growth Condition:

Distributed in bacterial strain : DH5-alpha T1 phage resistant
Antibiotic Selection: Host Type:  bacterial    Marker:  ampicillin
Host Type:  mammalian    Marker:  blasticidin
Bacterial Selection Condition:  100 ug/mL ampicillin
Growth Condition:  Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:  Commonly used conditions for ampicillin resistant plasmid clones.
Protein Expression Results: None
Recommended expression in: Not Applicable
       DyNA Vector Map

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Vector Name: pLenti6.2/V5-DEST

pLenti6.2V5-DEST in advanced viewed

Synonyms: pLenti6.2/V5-DEST
Sequencing Primer: Forward:  CMV forward
Reverse:  V5 reverse
Description: Lentiviral mammalian expression vector;CMV promoter and C-terminal V5 tag; ampicillin resistance in bacteria; blasticidin resistance in mammalian cells; Gateway recombinational cloning.
Comments: None
Size (bp): 8813
Parent Vector: None
Empty Vector: None
Properties: Gateway, acceptor (destination), lentiviral infection, mammalian expression, recombinational cloning, with tag/fusion/marker
Author Name: Invitrogen
Publications: None
Vector Map:         Vector Sequence:
Sequence:

 



Vector Features:

Type Name Description Start Position End Position
bacterial origin pUC ori pUC origin of replication 7733 8406
death cassette ccdB ccdB death cassette (removed when insert is present) 3767 3976
packaging signal HIV-1 psi HIV-1 psi packaging signal 521 565
poly-A signal SV40 SV40 polyadenylation signal 5639 5770
promoter RSV RSV/5' LTR hybrid promoter 1 410
promoter CMV CMV promoter driving gene of interest 1809 2392
promoter PGK PGK promoter to drive selection marker in mammalian cells 4266 4771
recombination site attR2 attR2 recombinational cloning site 4127 4142
recombination site attR1 attR1 recombinational cloning site 2440 2456
response element HIV-1 RRE HIV-1 Rev response element (RRE) 1075 1308
selectable marker AmpR Ampicillin resistance gene for selection in bacteria 6728 7588
selectable marker BlastR Blasticidin resistance gene in mammalian cells 4849 5249
selectable marker CmR chloramphenicol resistance marker (removed in plasmids with insert) 2673 3329
splice acceptor site 3' splice acceptor two 3' splice acceptors at 1656 and 1684 1656 1656
splice donor site 5' splice donor 5' splice donor 520 520
tag V5 C-terminal V5 epitope tag 4197 4238

 

  • Gene
  • Pathways
  • Protein
  • Clone
  • Organism
  • Genome
  • Reagents

Gene

 

Gene Symbol : ZNF394
Symbol Nomenclature : ZNF394
SYNONYM : ZKSCAN14; ZSCAN46
Designation : zinc finger protein 99|zinc finger protein with KRAB and SCAN domains 14
Full Nomenclature : zinc finger protein 394
GENEID : 84124
HGNC : 18832
Vega : OTTHUMG00000154660
Target GenBank: BC051847

 

Reference Sequence Alignment

  

HsCD00329725      ATGAATTCCAGCTTGACCGCCCAGAGGCGCGGCAGTGACGCCGAGTTGGGACCCTGGGTG
NM_032164.2       ATGAATTCCAGCTTGACCGCCCAGAGGCGCGGCAGTGACGCCGAGTTGGGACCCTGGGTG
                  ************************************************************

HsCD00329725      ATGGCTGCGAGGTCCAAGGACGCGGCGCCGTCCCAACGCGACGGACTTTTGCCCGTGAAA
NM_032164.2       ATGGCTGCGAGGTCCAAGGACGCGGCGCCGTCCCAACGCGACGGACTTTTGCCCGTGAAA
                  ************************************************************

HsCD00329725      GTGGAGGAAGACTCACCCGGAAGTTGGGAGCCCAACTATCCCGCGGCTTCGCCGGACCCC
NM_032164.2       GTGGAGGAAGACTCACCCGGAAGTTGGGAGCCCAACTATCCCGCGGCTTCGCCGGACCCC
                  ************************************************************

HsCD00329725      GAAACTTCTCGACTGCACTTTAGGCAGCTGCGTTACCAGGAGGTGGCTGGACCGGAAGAG
NM_032164.2       GAAACTTCTCGACTGCACTTTAGGCAGCTGCGTTACCAGGAGGTGGCTGGACCGGAAGAG
                  ************************************************************

HsCD00329725      GCGCTGAGCCGGCTCCGAGAACTCTGTCGTCGGTGGCTGAGACCCGAGCTGCTCTCCAAG
NM_032164.2       GCGCTGAGCCGGCTCCGAGAACTCTGTCGTCGGTGGCTGAGACCCGAGCTGCTCTCCAAG
                  ************************************************************

HsCD00329725      GAGCAGATCCTGGAGCTGCTGGTGCTGGAGCAGTTCCTCACCATCCTGCCCGAGGAGCTT
NM_032164.2       GAGCAGATCCTGGAGCTGCTGGTGCTGGAGCAGTTCCTCACCATCCTGCCCGAGGAGCTT
                  ************************************************************

HsCD00329725      CAAGCCTGGGTGCGAGAGCACTGCCCAGAGAGCGGGGAGGAGGCGGTGGCCGTGGTGCGG
NM_032164.2       CAAGCCTGGGTGCGAGAGCACTGCCCAGAGAGCGGGGAGGAGGCGGTGGCCGTGGTGCGG
                  ************************************************************

HsCD00329725      GCTCTGCAGCGAGCGCTCGATGGAACCTCATCCCAGGGGATGGTGACTTTCGAGGACACG
NM_032164.2       GCTCTGCAGCGAGCGCTCGATGGAACCTCATCCCAGGGGATGGTGACTTTCGAGGACACG
                  ************************************************************

HsCD00329725      GCTGTGTCTCTAACCTGGGAGGAGTGGGAGCGCCTGGACCCAGCACGGAGGGACTTCTGC
NM_032164.2       GCTGTGTCTCTAACCTGGGAGGAGTGGGAGCGCCTGGACCCAGCACGGAGGGACTTCTGC
                  ************************************************************

HsCD00329725      AGAGAGAGTGCGCAGAAGGATTCCGGGAGCACAGTTCCGCCGAGTTTGGAAAGCAGAGTG
NM_032164.2       AGAGAGAGTGCGCAGAAGGATTCCGGGAGCACAGTTCCGCCGAGTTTGGAAAGCAGAGTG
                  ************************************************************

HsCD00329725      GAGAACAAAGAGTTGATTCCAATGCAACAAATTTTAGAAGAAGCGGAGCCACAGGGGCAA
NM_032164.2       GAGAACAAAGAGTTGATTCCAATGCAACAAATTTTAGAAGAAGCGGAGCCACAGGGGCAA
                  ************************************************************

HsCD00329725      CTACAAGAAGCGTTCCAGGGGAAGCGCCCCCTGTTTTCTAAGTGTGGCAGTACCCATGAG
NM_032164.2       CTACAAGAAGCGTTCCAGGGGAAGCGCCCCCTGTTTTCTAAGTGTGGCAGTACCCATGAG
                  ************************************************************

HsCD00329725      GACAGGGTGGAAAAGCAGTCCGGAGACCCCTTGCCCCTGAAACTTGAAAATTCTCCTGAA
NM_032164.2       GACAGGGTGGAAAAGCAGTCCGGAGACCCCTTGCCCCTGAAACTTGAAAATTCTCCTGAA
                  ************************************************************

HsCD00329725      GCAGAAGGACTCAACAGCATCTCAGATGTCAATAAGAATGGTTCCATAGAAGGGGAAGAC
NM_032164.2       GCAGAAGGACTCAACAGCATCTCAGATGTCAATAAGAATGGTTCCATAGAAGGGGAAGAC
                  ************************************************************

HsCD00329725      TCTAAAAATAATGAATTGCAGAACAGTGCCAGGTGTTCCAACCTTGTTCTATGTCAGCAC
NM_032164.2       TCTAAAAATAATGAATTGCAGAACAGTGCCAGGTGTTCCAACCTTGTTCTATGTCAGCAC
                  ************************************************************

HsCD00329725      ATCCCGAAAGCAGAGAGGCCCACTGACAGTGAGGAACACGGGAACAAGTGCAAGCAAAGT
NM_032164.2       ATCCCGAAAGCAGAGAGGCCCACTGACAGTGAGGAACACGGGAACAAGTGCAAGCAAAGT
                  ************************************************************

HsCD00329725      TTCCACATGGTGACGTGGCACGTGCTGAAACCTCACAAGTCTGACAGTGGAGACAGTTTC
NM_032164.2       TTCCACATGGTGACGTGGCACGTGCTGAAACCTCACAAGTCTGACAGTGGAGACAGTTTC
                  ************************************************************

HsCD00329725      CATCATTCCAGCCTTTTTGAGACCCAGAGGCAGCTCCATGAAGAAAGACCTTATAAATGT
NM_032164.2       CATCATTCCAGCCTTTTTGAGACCCAGAGGCAGCTCCATGAAGAAAGACCTTATAAATGT
                  ************************************************************

HsCD00329725      GGTAACTGTGGGAAGAGTTTCAAACAACGCTCTGACCTCTTTAGACACCAGAGAATCCAC
NM_032164.2       GGTAACTGTGGGAAGAGTTTCAAACAACGCTCTGACCTCTTTAGACACCAGAGAATCCAC
                  ************************************************************

HsCD00329725      ACAGGTGAGAAACCCTATGGCTGCCAAGAATGTGGGAAAAGCTTCAGCCAGAGTGCTGCC
NM_032164.2       ACAGGTGAGAAACCCTATGGCTGCCAAGAATGTGGGAAAAGCTTCAGCCAGAGTGCTGCC
                  ************************************************************

HsCD00329725      CTGACCAAGCACCAGAGGACACACACAGGCGAGAAGCCGTACACCTGTCTGAAATGTGGG
NM_032164.2       CTGACCAAGCACCAGAGGACACACACAGGCGAGAAGCCGTACACCTGTCTGAAATGTGGG
                  ************************************************************

HsCD00329725      GAGCGCTTCAGGCAGAATTCACACCTAAATCGTCATCAAAGTACCCACAGTAGAGACAAA
NM_032164.2       GAGCGCTTCAGGCAGAATTCACACCTAAATCGTCATCAAAGTACCCACAGTAGAGACAAA
                  ************************************************************

HsCD00329725      CATTTTAAATGTGAGGAATGCGGGGAAACCTGTCATATTTCCAACCTTTTTAGACATCAG
NM_032164.2       CATTTTAAATGTGAGGAATGCGGGGAAACCTGTCATATTTCCAACCTTTTTAGACATCAG
                  ************************************************************

HsCD00329725      AGACTACATAAAGGGGAAAGACCCTATAAGTGTGAAGAATGCGAGAAGAGCTTCAAACAG
NM_032164.2       AGACTACATAAAGGGGAAAGACCCTATAAGTGTGAAGAATGCGAGAAGAGCTTCAAACAG
                  ************************************************************

HsCD00329725      CGCTCTGACCTCTTTAAACACCACAGAATCCACACTGGGGAGAAGCCCTATGGATGTTCC
NM_032164.2       CGCTCTGACCTCTTTAAACACCACAGAATCCACACTGGGGAGAAGCCCTATGGATGTTCC
                  ************************************************************

HsCD00329725      GTCTGTGGGAAACGCTTCAATCAGAGTGCAACCCTCATTAAACACCAGAGAATTCACACT
NM_032164.2       GTCTGTGGGAAACGCTTCAATCAGAGTGCAACCCTCATTAAACACCAGAGAATTCACACT
                  ************************************************************

HsCD00329725      GGGGAAAAGCCTTACAAATGTCTTGAATGTGGGGAAAGATTTAGACAAAGTACACACCTT
NM_032164.2       GGGGAAAAGCCTTACAAATGTCTTGAATGTGGGGAAAGATTTAGACAAAGTACACACCTT
                  ************************************************************

HsCD00329725      ATCCGACACCAAAGAATTCATCAAAATAAAGTGCTGTCGGCTGGGCGTGGTGGCTCACGC
NM_032164.2       ATCCGACACCAAAGAATTCATCAAAATAAAGTGCTGTCGGCTGGGCGTGGTGGCTCACGC
                  ************************************************************

HsCD00329725      CTATTG
NM_032164.2       CTATAA
                  ****:.

Pathways

Reactome : Gene Expression
Reactome : Generic Transcription Pathway

Protein

SMART domain : KRAB : krueppel associated box
SMART domain : SCAN : leucine rich region
SMART domain : ZnF_C2H2 : zinc finger
UniProt : Q53GI3
HPRD : 18333

Clone

Cloning Information : 268459
HIP Master Clone ID : 193482
Original Clone ID : FLH268459.01L

Organism


TAX_ID : 9606
Species Specific ID: 84124

Genome

Chromosome : 7
Map Location : 7q22.1
Ensembl : ENSG00000160908

Reagents

Labome : ZNF394-antibody