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TRIM45 (Homo sapiens) in pLenti6.2/V5-DEST (V5-tagged lentiviral mammalian expression vector)

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Explanation of Terms


Gene: Gene Symbol:  TRIM45
Gene Name:  tripartite motif containing 45
Sequence             
Original Clone ID: FLH268460.01L
Keyword: None
Species: Homo sapiens
Type: cDNA
Vector Name: pLenti6.2/V5-DEST               Format:  FUSION
Source: HIP
Description: None
Comments: None
Mutation/
Discrepancy :
No / Yes
Publications: None
Authors:
HsCD00329726

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AVAILABLE
No restriction
Special MTA: None

 

Insert sequence: 1689nts         Open reading frame : 1 to 1689
Reference Sequence Alignment

ATGTCAGAAAACAGAAAAACGCTGCTGGGCTTTGTAAGCAAACTCACTAGTGGGACTGCA
CTTGGGAACTCAGGCAAGACTCACTGCCCCCTGTGCTTGGGGCTTTTCAAAGCCCCCAGG
CTCTTGCCTTGTTTGCATACAGTTTGCACCACGTGTCTGGAGCAGCTGGAGCCCTTCTCA
GTAGTGGACATCCGAGGGGGAGACTCTGACACAAGCTCTGAGGGGTCAATATTCCAGGAA
CTCAAGCCACGAAGTCTGCAGTCGCAGATCGGCATCCTTTGTCCTGTATGTGATGCTCAG
GTGGACCTGCCCATGGGTGGAGTGAAGGCTTTAACCATAGACCACCTGGCCGTGAATGAT
GTGATGCTGGAGAGCCTACGTGGGGAAGGCCAGGGCCTGGTGTGTGACCTGTGCAACGAC
AGGGAAGTAGAGAAGAGGTGTCAGACCTGCAAAGCCAACCTCTGCCACTTCTGCTGCCAG
GCTCATAGGCGGCAGAAGAAAACGACTTACCACACCATGGTGGACCTAAAAGACTTGAAA
GGCTACAGCCGGATTGGGAAGCCCATCCTGTGTCCTGTTCACCCTGCAGAGGAACTGAGG
CTGTTCTGTGAGTTCTGTGACCGGCCCGTGTGCCAGGATTGTGTGGTGGGGGAGCATCGG
GAACACCCCTGTGACTTCACCAGCAATGTCATCCACAAGCATGGGGACTCTGTGTGGGAG
CTCCTCAAAGATACTCAGCCCCACGTGGAGGCCCTGGAGGAAGCCCTGGCTCAGATCCAC
ATAATAAACAGTGCCCTCCAGAAGCGAGTGGAGGCAGTGGCAGCTGATGTCCGGACATTC
TCGGAGGGCTACATTAAGGCCATTGAGGAGCATCGGGACAAGCTGCTGAAGCAGCTGGAA
GACATACGGGCCCAGAAGGAAAATTCCCTGCAGCTGCAGAAGGCCCAGCTGGAACAGTTA
CTGGCAGACATGCGGACTGGAGTGGAGTTCACCGAGCACTTGCTGACCAGCGGCTCAGAC
TTGGAGATCCTCATCACCAAGAGGGTGGTGGTAGAACGGCTCAGGAAGCTGAACAAAGTT
CAATATAGCACCCGTCCTGGAGTAAATGATAAGATACGCTTCTGTCCTCAGGAGAAAGCA
GGCCAGTGCCGTGGCTATGAAATTTATGACCTCCACAGAGCCCGGGAGAAACAGACGGCC
TCTTTCACCCTGCTTTGTAAGGATGCCGCAGGAGAAATCATGGGCAGGGGAGGAGACAAC
GTTCAAGTTGCCGTTGTCCCTAAAGATAAGAAAGACAGCCCAGTCAGAACAATGGTCCAG
GATAACAAGGATGGGACATACTACATTTCCTACACCCCCAAGGAACCTGGCGTCTATACT
GTGTGGGTCTGCATCAAAGAACAGCATGTGCAGGGCTCGCCATTCACTGTGACGGTGAGG
AGAAAGCACCGCCCACACTCAGGCGTGTTTCACTGCTGCACCTTCTGCTCCAGCGGGGGC
CAGAAAACCGCTCGCTGCGCCTGTGGAGGCACCATGCCAGGTGGGTACCTAGGCTGTGGC
CATGGACACAAAGGCCACCCAGGTCATCCCCACTGGTCATGCTGTGGAAAATTTAATGAG
AAATCTGAATGCACATGGACAGGTGGGCAGAGCGCACCGAGGAGTCTACTTAGGACTGTG
GCTCTCTTG


Coding Sequence Details
Insert Sequence Verified?: N Verification Method: Insert was fully sequenced in parent vector
Reference Sequence Annotations:
End on reference sequence 2255
Start on reference sequence 567
Mutations:
None
Discrepancy:
c19a,P7T; g731a,G244D
                                

5' Linker Sequence:
5p GTACAAAAAAGCAGGCTCCACC(atg-goi)

3' Linker Sequence:
5p (goi-stop)GACCCAGCTTTCTTGTAC

 

 Recommended Growth Condition:

Distributed in bacterial strain : DH5-alpha T1 phage resistant
Antibiotic Selection: Host Type:  bacterial    Marker:  ampicillin
Host Type:  mammalian    Marker:  blasticidin
Bacterial Selection Condition:  100 ug/mL ampicillin
Growth Condition:  Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:  Commonly used conditions for ampicillin resistant plasmid clones.
Protein Expression Results: None
Recommended expression in: Not Applicable
       DyNA Vector Map

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Vector Name: pLenti6.2/V5-DEST

pLenti6.2V5-DEST in advanced viewed

Synonyms: pLenti6.2/V5-DEST
Sequencing Primer: Forward:  CMV forward
Reverse:  V5 reverse
Description: Lentiviral mammalian expression vector;CMV promoter and C-terminal V5 tag; ampicillin resistance in bacteria; blasticidin resistance in mammalian cells; Gateway recombinational cloning.
Comments: None
Size (bp): 8813
Parent Vector: None
Empty Vector: None
Properties: Gateway, acceptor (destination), lentiviral infection, mammalian expression, recombinational cloning, with tag/fusion/marker
Author Name: Invitrogen
Publications: None
Vector Map:         Vector Sequence:
Sequence:

 



Vector Features:

Type Name Description Start Position End Position
bacterial origin pUC ori pUC origin of replication 7733 8406
death cassette ccdB ccdB death cassette (removed when insert is present) 3767 3976
packaging signal HIV-1 psi HIV-1 psi packaging signal 521 565
poly-A signal SV40 SV40 polyadenylation signal 5639 5770
promoter RSV RSV/5' LTR hybrid promoter 1 410
promoter CMV CMV promoter driving gene of interest 1809 2392
promoter PGK PGK promoter to drive selection marker in mammalian cells 4266 4771
recombination site attR2 attR2 recombinational cloning site 4127 4142
recombination site attR1 attR1 recombinational cloning site 2440 2456
response element HIV-1 RRE HIV-1 Rev response element (RRE) 1075 1308
selectable marker AmpR Ampicillin resistance gene for selection in bacteria 6728 7588
selectable marker BlastR Blasticidin resistance gene in mammalian cells 4849 5249
selectable marker CmR chloramphenicol resistance marker (removed in plasmids with insert) 2673 3329
splice acceptor site 3' splice acceptor two 3' splice acceptors at 1656 and 1684 1656 1656
splice donor site 5' splice donor 5' splice donor 520 520
tag V5 C-terminal V5 epitope tag 4197 4238

 

  • Gene
  • Protein
  • Clone
  • Organism
  • Genome
  • Reagents

Gene

 

Gene Symbol : TRIM45
Symbol Nomenclature : TRIM45
SYNONYM : RNF99
Designation : ring finger protein 99|tripartite motif-containing 45|tripartite motif-containing protein 45
Full Nomenclature : tripartite motif containing 45
GENEID : 80263
Locus Tag : RP11-229A19.2
HGNC : 19018
MIM : 609318
Vega : OTTHUMG00000012119
Target GenBank: BC034943

 

Reference Sequence Alignment

  

HsCD00329726        ATGTCAGAAAACAGAAAAACGCTGCTGGGCTTTGTAAGCAAACTCACTAGTGGGACTGCA
NM_025188.3         ATGTCAGAAAACAGAAAACCGCTGCTGGGCTTTGTAAGCAAACTCACTAGTGGGACTGCA
NM_001145635.1      ATGTCAGAAAACAGAAAACCGCTGCTGGGCTTTGTAAGCAAACTCACTAGTGGGACTGCA
                    ******************.*****************************************

HsCD00329726        CTTGGGAACTCAGGCAAGACTCACTGCCCCCTGTGCTTGGGGCTTTTCAAAGCCCCCAGG
NM_025188.3         CTTGGGAACTCAGGCAAGACTCACTGCCCCCTGTGCTTGGGGCTTTTCAAAGCCCCCAGG
NM_001145635.1      CTTGGGAACTCAGGCAAGACTCACTGCCCCCTGTGCTTGGGGCTTTTCAAAGCCCCCAGG
                    ************************************************************

HsCD00329726        CTCTTGCCTTGTTTGCATACAGTTTGCACCACGTGTCTGGAGCAGCTGGAGCCCTTCTCA
NM_025188.3         CTCTTGCCTTGTTTGCATACAGTTTGCACCACGTGTCTGGAGCAGCTGGAGCCCTTCTCA
NM_001145635.1      CTCTTGCCTTGTTTGCATACAGTTTGCACCACGTGTCTGGAGCAGCTGGAGCCCTTCTCA
                    ************************************************************

HsCD00329726        GTAGTGGACATCCGAGGGGGAGACTCTGACACAAGCTCTGAGGGGTCAATATTCCAGGAA
NM_025188.3         GTAGTGGACATCCGAGGGGGAGACTCTGACACAAGCTCTGAGGGGTCAATATTCCAGGAA
NM_001145635.1      GTAGTGGACATCCGAGGGGGAGACTCTGACACAAGCTCTGAGGGGTCAATATTCCAGGAA
                    ************************************************************

HsCD00329726        CTCAAGCCACGAAGTCTGCAGTCGCAGATCGGCATCCTTTGTCCTGTATGTGATGCTCAG
NM_025188.3         CTCAAGCCACGAAGTCTGCAGTCGCAGATCGGCATCCTTTGTCCTGTATGTGATGCTCAG
NM_001145635.1      CTCAAGCCACGAAGTCTGCAGTCGCAGATCGGCATCCTTTGTCCTGTATGTGATGCTCAG
                    ************************************************************

HsCD00329726        GTGGACCTGCCCATGGGTGGAGTGAAGGCTTTAACCATAGACCACCTGGCCGTGAATGAT
NM_025188.3         GTGGACCTGCCCATGGGTGGAGTGAAGGCTTTAACCATAGACCACCTGGCCGTGAATGAT
NM_001145635.1      GTGGACCTGCCCATGGGTGGAGTGAAGGCTTTAACCATAGACCACCTGGCCGTGAATGAT
                    ************************************************************

HsCD00329726        GTGATGCTGGAGAGCCTACGTGGGGAAGGCCAGGGCCTGGTGTGTGACCTGTGCAACGAC
NM_025188.3         GTGATGCTGGAGAGCCTACGTGGGGAAGGCCAGGGCCTGGTGTGTGACCTGTGCAACGAC
NM_001145635.1      GTGATGCTGGAGAGCCTACGTGGGGAAGGCCAGGGCCTGGTGTGTGACCTGTGCAACGAC
                    ************************************************************

HsCD00329726        AGGGAAGTAGAGAAGAGGTGTCAGACCTGCAAAGCCAACCTCTGCCACTTCTGCTGCCAG
NM_025188.3         AGGGAAGTAGAGAAGAGGTGTCAGACCTGCAAAGCCAACCTCTGCCACTTCTGCTGCCAG
NM_001145635.1      AGGGAAGTAGAGAAGAGGTGTCAGACCTGCAAAGCCAACCTCTGCCACTTCTGCTGCCAG
                    ************************************************************

HsCD00329726        GCTCATAGGCGGCAGAAGAAAACGACTTACCACACCATGGTGGACCTAAAAGACTTGAAA
NM_025188.3         GCTCATAGGCGGCAGAAGAAAACGACTTACCACACCATGGTGGACCTAAAAGACTTGAAA
NM_001145635.1      GCTCATAGGCGGCAGAAGAAAACGACTTACCACACCATGGTGGACCTAAAAGACTTGAAA
                    ************************************************************

HsCD00329726        GGCTACAGCCGGATTGGGAAGCCCATCCTGTGTCCTGTTCACCCTGCAGAGGAACTGAGG
NM_025188.3         GGCTACAGCCGGATTGGGAAGCCCATCCTGTGTCCTGTTCACCCTGCAGAGGAACTGAGG
NM_001145635.1      GGCTACAGCCGGATTGGGAAGCCCATCCTGTGTCCTGTTCACCCTGCAGAGGAACTGAGG
                    ************************************************************

HsCD00329726        CTGTTCTGTGAGTTCTGTGACCGGCCCGTGTGCCAGGATTGTGTGGTGGGGGAGCATCGG
NM_025188.3         CTGTTCTGTGAGTTCTGTGACCGGCCCGTGTGCCAGGATTGTGTGGTGGGGGAGCATCGG
NM_001145635.1      CTGTTCTGTGAGTTCTGTGACCGGCCCGTGTGCCAGGATTGTGTGGTGGGGGAGCATCGG
                    ************************************************************

HsCD00329726        GAACACCCCTGTGACTTCACCAGCAATGTCATCCACAAGCATGGGGACTCTGTGTGGGAG
NM_025188.3         GAACACCCCTGTGACTTCACCAGCAATGTCATCCACAAGCATGGGGACTCTGTGTGGGAG
NM_001145635.1      GAACACCCCTGTGACTTCACCAGCAATGTCATCCACAAGCATGGGGACTCTGTGTGGGAG
                    ************************************************************

HsCD00329726        CTCCTCAAAGATACTCAGCCCCACGTGGAGGCCCTGGAGGAAGCCCTGGCTCAGATCCAC
NM_025188.3         CTCCTCAAAGGTACTCAGCCCCACGTGGAGGCCCTGGAGGAAGCCCTGGCTCAGATCCAC
NM_001145635.1      CTCCTCAAAGGTACTCAGCCCCACGTGGAGGCCCTGGAGGAAGCCCTGGCTCAGATCCAC
                    **********.*************************************************

HsCD00329726        ATAATAAACAGTGCCCTCCAGAAGCGAGTGGAGGCAGTGGCAGCTGATGTCCGGACATTC
NM_025188.3         ATAATAAACAGTGCCCTCCAGAAGCGAGTGGAGGCAGTGGCAGCTGATGTCCGGACATTC
NM_001145635.1      ATAATAAACAGTGCCCTCCAGAAGCGAGTGGAGGCAGTGGCAGCTGATGTCCGGACATTC
                    ************************************************************

HsCD00329726        TCGGAGGGCTACATTAAGGCCATTGAGGAGCATCGGGACAAGCTGCTGAAGCAGCTGGAA
NM_025188.3         TCGGAGGGCTACATTAAGGCCATTGAGGAGCATCGGGACAAGCTGCTGAAGCAGCTGGAA
NM_001145635.1      TCGGAGGGCTACATTAAGGCCATTGAGGAGCATCGGGACAAGCTGCTGAAGCAGCTGGAA
                    ************************************************************

HsCD00329726        GACATACGGGCCCAGAAGGAAAATTCCCTGCAGCTGCAGAAGGCCCAGCTGGAACAGTTA
NM_025188.3         GACATACGGGCCCAGAAGGAAAATTCCCTGCAGCTGCAGAAGGCCCAGCTGGAACAGTTA
NM_001145635.1      GACATACGGGCCCAGAAGGAAAATTCCCTGCAGCTGCAGAAGGCCCAGCTGGAACAGTTA
                    ************************************************************

HsCD00329726        CTGGCAGACATGCGGACTGGAGTGGAGTTCACCGAGCACTTGCTGACCAGCGGCTCAGAC
NM_025188.3         CTGGCAGACATGCGGACTGGAGTGGAGTTCACCGAGCACTTGCTGACCAGCGGCTCAGAC
NM_001145635.1      CTGGCAGACATGCGGACTGGAGTGGAGTTCACCGAGCACTTGCTGACCAGCGGCTCAGAC
                    ************************************************************

HsCD00329726        TTGGAGATCCTCATCACCAAGAGGGTGGTGGTAGAACGGCTCAGGAAGCTGAACAAAGTT
NM_025188.3         TTGGAGATCCTCATCACCAAGAGGGTGGTGGTAGAACGGCTCAGGAAGCTGAACAAAGTT
NM_001145635.1      TTGGAGATCCTCATCACCAAGAGGGTGGTGGTAGAACGGCTCAGGAAGCTGAACAAAGTT
                    ************************************************************

HsCD00329726        CAATATAGCACCCGTCCTGGAGTAAATGATAAGATACGCTTCTGTCCTCAGGAGAAAGCA
NM_025188.3         CAATATAGCACCCGTCCTGGAGTAAATGATAAGATACGCTTCTGTCCTCAGGAGAAAGCA
NM_001145635.1      CAATATAGCACCCGTCCTGGAGTAAATGATAAGATACGCTTCTGTCCTCAGGAGAAAGCA
                    ************************************************************

HsCD00329726        GGCCAGTGCCGTGGCTATGAAATTTATG--------------------------------
NM_025188.3         GGCCAGTGCCGTGGCTATGAAATTTATGGTACGATTAATACCAAAGAGGTTGATCCAGCC
NM_001145635.1      GGCCAGTGCCGTGGCTATGAAATTTATG--------------------------------
                    ****************************                                

HsCD00329726        ----------------------ACCTCCACAGAGCCCGGGAGAAACAGACGGCCTCTTTC
NM_025188.3         AAATGTGTCCTACAAGGAGAAGACCTCCACAGAGCCCGGGAGAAACAGACGGCCTCTTTC
NM_001145635.1      ----------------------ACCTCCACAGAGCCCGGGAGAAACAGACGGCCTCTTTC
                                          **************************************

HsCD00329726        ACCCTGCTTTGTAAGGATGCCGCAGGAGAAATCATGGGCAGGGGAGGAGACAACGTTCAA
NM_025188.3         ACCCTGCTTTGTAAGGATGCCGCAGGAGAAATCATGGGCAGGGGAGGAGACAACGTTCAA
NM_001145635.1      ACCCTGCTTTGTAAGGATGCCGCAGGAGAAATCATGGGCAGGGGAGGAGACAACGTTCAA
                    ************************************************************

HsCD00329726        GTTGCCGTTGTCCCTAAAGATAAGAAAGACAGCCCAGTCAGAACAATGGTCCAGGATAAC
NM_025188.3         GTTGCCGTTGTCCCTAAAGATAAGAAAGACAGCCCAGTCAGAACAATGGTCCAGGATAAC
NM_001145635.1      GTTGCCGTTGTCCCTAAAGATAAGAAAGACAGCCCAGTCAGAACAATGGTCCAGGATAAC
                    ************************************************************

HsCD00329726        AAGGATGGGACATACTACATTTCCTACACCCCCAAGGAACCTGGCGTCTATACTGTGTGG
NM_025188.3         AAGGATGGGACATACTACATTTCCTACACCCCCAAGGAACCTGGCGTCTATACTGTGTGG
NM_001145635.1      AAGGATGGGACATACTACATTTCCTACACCCCCAAGGAACCTGGCGTCTATACTGTGTGG
                    ************************************************************

HsCD00329726        GTCTGCATCAAAGAACAGCATGTGCAGGGCTCGCCATTCACTGTGACGGTGAGGAGAAAG
NM_025188.3         GTCTGCATCAAAGAACAGCATGTGCAGGGCTCGCCATTCACTGTGATGGTGAGGAGAAAG
NM_001145635.1      GTCTGCATCAAAGAACAGCATGTGCAGGGCTCGCCATTCACTGTGATGGTGAGGAGAAAG
                    ********************************************** *************

HsCD00329726        CACCGCCCACACTCAGGCGTGTTTCACTGCTGCACCTTCTGCTCCAGCGGGGGCCAGAAA
NM_025188.3         CACCGCCCACACTCAGGCGTGTTTCACTGCTGCACCTTCTGCTCCAGCGGGGGCCAGAAA
NM_001145635.1      CACCGCCCACACTCAGGCGTGTTTCACTGCTGCACCTTCTGCTCCAGCGGGGGCCAGAAA
                    ************************************************************

HsCD00329726        ACCGCTCGCTGCGCCTGTGGAGGCACCATGCCAGGTGGGTACCTAGGCTGTGGCCATGGA
NM_025188.3         ACCGCTCGCTGCGCCTGTGGAGGCACCATGCCAGGTGGGTACCTAGGCTGTGGCCATGGA
NM_001145635.1      ACCGCTCGCTGCGCCTGTGGAGGCACCATGCCAGGTGGGTACCTAGGCTGTGGCCATGGA
                    ************************************************************

HsCD00329726        CACAAAGGCCACCCAGGTCATCCCCACTGGTCATGCTGTGGAAAATTTAATGAGAAATCT
NM_025188.3         CACAAAGGCCACCCAGGTCATCCCCACTGGTCATGCTGTGGAAAATTTAATGAGAAATCT
NM_001145635.1      CACAAAGGCCACCCAGGTCATCCCCACTGGTCATGCTGTGGAAAATTTAATGAGAAATCT
                    ************************************************************

HsCD00329726        GAATGCACATGGACAGGTGGGCAGAGCGCACCGAGGAGTCTACTTAGGACTGTGGCTCTC
NM_025188.3         GAATGCACATGGACAGGTGGGCAGAGCGCACCGAGGAGTCTACTTAGGACTGTGGCTCTC
NM_001145635.1      GAATGCACATGGACAGGTGGGCAGAGCGCACCGAGGAGTCTACTTAGGACTGTGGCTCTC
                    ************************************************************

HsCD00329726        TTG
NM_025188.3         TGA
NM_001145635.1      TGA
                    * .

Clone

Cloning Information : 268460
HIP Master Clone ID : 193484
Original Clone ID : FLH268460.01L

Organism


TAX_ID : 9606
Species Specific ID: 80263

Genome

Chromosome : 1
Map Location : 1p13.1
Ensembl : ENSG00000134253

Reagents

Labome : TRIM45-antibody