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TCEA2 (Homo sapiens) in pLenti6.2/V5-DEST (V5-tagged lentiviral mammalian expression vector)

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Explanation of Terms


Gene: Gene Symbol:  TCEA2
Gene Name:  transcription elongation factor A (SII), 2
Sequence             
Original Clone ID: FLH268461.01L
Keyword: None
Species: Homo sapiens
Type: cDNA
Vector Name: pLenti6.2/V5-DEST               Format:  FUSION
Source: HIP
Description: None
Comments: None
Mutation/
Discrepancy :
No / Yes
Publications: None
Authors:
HsCD00329727

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AVAILABLE
No restriction
Special MTA: None

 

Insert sequence: 900nts         Open reading frame : 1 to 900
Reference Sequence Alignment

ATGATGGGCAAGGAAGAGGAGATTGCGCGGATCGCCCGGAGGCTGGACAAGATGGTGACC
AAGAAGAGCGCGGAGGGAGCCATGGATTTGCTGCGGGAGCTGAAGGCCATGCCTATCACG
CTGCACCTGCTCCAGTCCACCCGAGTCGGGATGTCTGTCAACGCCCTTCGGAAGCAGAGC
TCGGATGAGGAGGTCATTGCACTGGCCAAGTCTCTCATCAAGTCCTGGAAGAAGCTCCTG
GATGCTTCCGATGCCAAAGCCAGGGAGCGGGGGAGGGGCATGCCTCTGCCCACGTCCTCG
AGGGATGCCTCAGAGGCCCCGGATCCCAGCCGCAAGAGGCCGGAGCTGCCCAGGGCACCG
TCGACTCCGAGGATCACCACATTTCCTCCGGTGCCTGTCACCTGTGATGCCGTGCGCAAC
AAGTGCCGCGAGATGCTGACCGCTGCCCTGCAGACGGACCATGACCACGTGGCCATCGGT
GCGGACTGCGAGCGCCTGTCGGCTCAGATCGAGGAATGCATCTTCCGGGACGTTGGAAAC
ACAGACATGAAGTATAAGAACCGTGTACGGAGTCGTATCTCCAACCTGAAGGATGCCAAG
AACCCTGACCTGCGGCGGAATGTGCTGTGTGGGGCCATAACACCCCAGCAGATCGCTGTG
ATGACCTCAGAGGAGATGGCCAGTGATGAGCTGAAGGAGATCCGTAAGGCCATGACCAAG
GAGGCCATCCGAGAGCACCAGATGGCCCGCACTGGCGGCACGCAGACAGACCTGTTCACC
TGCGGCAAGTGCAGGAAAAAGAACTGCACCTACACACAGGTGCAGACCCGCAGCTCTGAT
GAGCCCATGACCACCTTTGTTGTCTGCAACGAGTGTGGCAACCGCTGGAAGTTCTGCTTG


Coding Sequence Details
Insert Sequence Verified?: N Verification Method: Insert was fully sequenced in parent vector
Reference Sequence Annotations:
End on reference sequence 1011
Start on reference sequence 112
Mutations:
None
Discrepancy:
a879c
                                

5' Linker Sequence:
5p GTACAAAAAAGCAGGCTCCACC(atg-goi)

3' Linker Sequence:
5p (goi-stop)GACCCAGCTTTCTTGTAC

 

 Recommended Growth Condition:

Distributed in bacterial strain : DH5-alpha T1 phage resistant
Antibiotic Selection: Host Type:  bacterial    Marker:  ampicillin
Host Type:  mammalian    Marker:  blasticidin
Bacterial Selection Condition:  100 ug/mL ampicillin
Growth Condition:  Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:  Commonly used conditions for ampicillin resistant plasmid clones.
Protein Expression Results: None
Recommended expression in: Not Applicable
       DyNA Vector Map

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Vector Name: pLenti6.2/V5-DEST

pLenti6.2V5-DEST in advanced viewed

Synonyms: pLenti6.2/V5-DEST
Sequencing Primer: Forward:  CMV forward
Reverse:  V5 reverse
Description: Lentiviral mammalian expression vector;CMV promoter and C-terminal V5 tag; ampicillin resistance in bacteria; blasticidin resistance in mammalian cells; Gateway recombinational cloning.
Comments: None
Size (bp): 8813
Parent Vector: None
Empty Vector: None
Properties: Gateway, acceptor (destination), lentiviral infection, mammalian expression, recombinational cloning, with tag/fusion/marker
Author Name: Invitrogen
Publications: None
Vector Map:         Vector Sequence:
Sequence:

 



Vector Features:

Type Name Description Start Position End Position
bacterial origin pUC ori pUC origin of replication 7733 8406
death cassette ccdB ccdB death cassette (removed when insert is present) 3767 3976
packaging signal HIV-1 psi HIV-1 psi packaging signal 521 565
poly-A signal SV40 SV40 polyadenylation signal 5639 5770
promoter RSV RSV/5' LTR hybrid promoter 1 410
promoter CMV CMV promoter driving gene of interest 1809 2392
promoter PGK PGK promoter to drive selection marker in mammalian cells 4266 4771
recombination site attR2 attR2 recombinational cloning site 4127 4142
recombination site attR1 attR1 recombinational cloning site 2440 2456
response element HIV-1 RRE HIV-1 Rev response element (RRE) 1075 1308
selectable marker AmpR Ampicillin resistance gene for selection in bacteria 6728 7588
selectable marker BlastR Blasticidin resistance gene in mammalian cells 4849 5249
selectable marker CmR chloramphenicol resistance marker (removed in plasmids with insert) 2673 3329
splice acceptor site 3' splice acceptor two 3' splice acceptors at 1656 and 1684 1656 1656
splice donor site 5' splice donor 5' splice donor 520 520
tag V5 C-terminal V5 epitope tag 4197 4238

 

  • Gene
  • Protein
  • Clone
  • Organism
  • Genome
  • Reagents

Gene

 

Gene Symbol : TCEA2
Symbol Nomenclature : TCEA2
SYNONYM : TFIIS
Designation : testis-specific S-II|transcription elongation factor A protein 2|transcription elongation factor S-II protein 2|transcription elongation factor TFIIS.1|transcription elongation factor TFIIS.l
Full Nomenclature : transcription elongation factor A (SII), 2
GENEID : 6919
Locus Tag : RP11-299N6.2
HGNC : 11614
MIM : 604784
Vega : OTTHUMG00000033026
Target GenBank: BC018896

 

Reference Sequence Alignment

  

HsCD00329727      ATGATGGGCAAGGAAGAGGAGATTGCGCGGATCGCCCGGAGGCTGGACAAGATGGTGACC
NM_003195.4       ATGATGGGCAAGGAAGAGGAGATTGCGCGGATCGCCCGGAGGCTGGACAAGATGGTGACC
NM_198723.1       ------------------------------------------------------------
                                                                              

HsCD00329727      AAGAAGAGCGCGGAGGGAGCCATGGATTTGCTGCGGGAGCTGAAGGCCATGCCTATCACG
NM_003195.4       AAGAAGAGCGCGGAGGGAGCCATGGATTTGCTGCGGGAGCTGAAGGCCATGCCTATCACG
NM_198723.1       ---------------------ATGGATTTGCTGCGGGAGCTGAAGGCCATGCCTATCACG
                                       ***************************************

HsCD00329727      CTGCACCTGCTCCAGTCCACCCGAGTCGGGATGTCTGTCAACGCCCTTCGGAAGCAGAGC
NM_003195.4       CTGCACCTGCTCCAGTCCACCCGAGTCGGGATGTCTGTCAACGCCCTTCGGAAGCAGAGC
NM_198723.1       CTGCACCTGCTCCAGTCCACCCGAGTCGGGATGTCTGTCAACGCCCTTCGGAAGCAGAGC
                  ************************************************************

HsCD00329727      TCGGATGAGGAGGTCATTGCACTGGCCAAGTCTCTCATCAAGTCCTGGAAGAAGCTCCTG
NM_003195.4       TCGGATGAGGAGGTCATTGCACTGGCCAAGTCTCTCATCAAGTCCTGGAAGAAGCTCCTG
NM_198723.1       TCGGATGAGGAGGTCATTGCACTGGCCAAGTCTCTCATCAAGTCCTGGAAGAAGCTCCTG
                  ************************************************************

HsCD00329727      GATGCTTCCGATGCCAAAGCCAGGGAGCGGGGGAGGGGCATGCCTCTGCCCACGTCCTCG
NM_003195.4       GATGCTTCCGATGCCAAAGCCAGGGAGCGGGGGAGGGGCATGCCTCTGCCCACGTCCTCG
NM_198723.1       GATGCTTCCGATGCCAAAGCCAGGGAGCGGGGGAGGGGCATGCCTCTGCCCACGTCCTCG
                  ************************************************************

HsCD00329727      AGGGATGCCTCAGAGGCCCCGGATCCCAGCCGCAAGAGGCCGGAGCTGCCCAGGGCACCG
NM_003195.4       AGGGATGCCTCAGAGGCCCCGGATCCCAGCCGCAAGAGGCCGGAGCTGCCCAGGGCACCG
NM_198723.1       AGGGATGCCTCAGAGGCCCCGGATCCCAGCCGCAAGAGGCCGGAGCTGCCCAGGGCACCG
                  ************************************************************

HsCD00329727      TCGACTCCGAGGATCACCACATTTCCTCCGGTGCCTGTCACCTGTGATGCCGTGCGCAAC
NM_003195.4       TCGACTCCGAGGATCACCACATTTCCTCCGGTGCCTGTCACCTGTGATGCCGTGCGCAAC
NM_198723.1       TCGACTCCGAGGATCACCACATTTCCTCCGGTGCCTGTCACCTGTGATGCCGTGCGCAAC
                  ************************************************************

HsCD00329727      AAGTGCCGCGAGATGCTGACCGCTGCCCTGCAGACGGACCATGACCACGTGGCCATCGGT
NM_003195.4       AAGTGCCGCGAGATGCTGACCGCTGCCCTGCAGACGGACCATGACCACGTGGCCATCGGT
NM_198723.1       AAGTGCCGCGAGATGCTGACCGCTGCCCTGCAGACGGACCATGACCACGTGGCCATCGGT
                  ************************************************************

HsCD00329727      GCGGACTGCGAGCGCCTGTCGGCTCAGATCGAGGAATGCATCTTCCGGGACGTTGGAAAC
NM_003195.4       GCGGACTGCGAGCGCCTGTCGGCTCAGATCGAGGAATGCATCTTCCGGGACGTTGGAAAC
NM_198723.1       GCGGACTGCGAGCGCCTGTCGGCTCAGATCGAGGAATGCATCTTCCGGGACGTTGGAAAC
                  ************************************************************

HsCD00329727      ACAGACATGAAGTATAAGAACCGTGTACGGAGTCGTATCTCCAACCTGAAGGATGCCAAG
NM_003195.4       ACAGACATGAAGTATAAGAACCGTGTACGGAGTCGTATCTCCAACCTGAAGGATGCCAAG
NM_198723.1       ACAGACATGAAGTATAAGAACCGTGTACGGAGTCGTATCTCCAACCTGAAGGATGCCAAG
                  ************************************************************

HsCD00329727      AACCCTGACCTGCGGCGGAATGTGCTGTGTGGGGCCATAACACCCCAGCAGATCGCTGTG
NM_003195.4       AACCCTGACCTGCGGCGGAATGTGCTGTGTGGGGCCATAACACCCCAGCAGATCGCTGTG
NM_198723.1       AACCCTGACCTGCGGCGGAATGTGCTGTGTGGGGCCATAACACCCCAGCAGATCGCTGTG
                  ************************************************************

HsCD00329727      ATGACCTCAGAGGAGATGGCCAGTGATGAGCTGAAGGAGATCCGTAAGGCCATGACCAAG
NM_003195.4       ATGACCTCAGAGGAGATGGCCAGTGATGAGCTGAAGGAGATCCGTAAGGCCATGACCAAG
NM_198723.1       ATGACCTCAGAGGAGATGGCCAGTGATGAGCTGAAGGAGATCCGTAAGGCCATGACCAAG
                  ************************************************************

HsCD00329727      GAGGCCATCCGAGAGCACCAGATGGCCCGCACTGGCGGCACGCAGACAGACCTGTTCACC
NM_003195.4       GAGGCCATCCGAGAGCACCAGATGGCCCGCACTGGCGGCACGCAGACAGACCTGTTCACC
NM_198723.1       GAGGCCATCCGAGAGCACCAGATGGCCCGCACTGGCGGCACGCAGACAGACCTGTTCACC
                  ************************************************************

HsCD00329727      TGCGGCAAGTGCAGGAAAAAGAACTGCACCTACACACAGGTGCAGACCCGCAGCTCTGAT
NM_003195.4       TGCGGCAAGTGCAGGAAAAAGAACTGCACCTACACACAGGTGCAGACCCGCAGCTCTGAT
NM_198723.1       TGCGGCAAGTGCAGGAAAAAGAACTGCACCTACACACAGGTGCAGACCCGCAGCTCTGAT
                  ************************************************************

HsCD00329727      GAGCCCATGACCACCTTTGTTGTCTGCAACGAGTGTGGCAACCGCTGGAAGTTCTGCTTG
NM_003195.4       GAGCCCATGACCACCTTTGTTGTCTGCAACGAGTGTGGAAACCGCTGGAAGTTCTGCTGA
NM_198723.1       GAGCCCATGACCACCTTTGTTGTCTGCAACGAGTGTGGAAACCGCTGGAAGTTCTGCTGA
                  **************************************.******************* .

Clone

Cloning Information : 268461
HIP Master Clone ID : 193485
Original Clone ID : FLH268461.01L

Organism


TAX_ID : 9606
Species Specific ID: 6919

Genome

Chromosome : 20
Map Location : 20q13.33
Ensembl : ENSG00000171703

Reagents

Labome : TCEA2-antibody