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ZNF503 (Homo sapiens) in pLenti6.2/V5-DEST (V5-tagged lentiviral mammalian expression vector)

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Explanation of Terms


Gene: Gene Symbol:  ZNF503
Gene Name:  zinc finger protein 503
Sequence             
Original Clone ID: FLH268464.01L
Keyword: None
Species: Homo sapiens
Type: cDNA
Vector Name: pLenti6.2/V5-DEST               Format:  FUSION
Source: HIP
Description: None
Comments: None
Mutation/
Discrepancy :
No / No
Publications: None
Authors:
HsCD00329729

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AVAILABLE
No restriction
Special MTA: None

 

Insert sequence: 1941nts         Open reading frame : 1 to 1941
Reference Sequence Alignment

ATGAGCACAGCGCCCTCGCTTTCTGCCCTAAGAAGCAGTAAGCACAGCGGCGGCGGCGGC
GGCGGAGGCGGAGGCGGCGGTGCAGACCCTGCCTGGACCAGCGCGCTCTCTGGAAATAGC
TCCGGCCCCGGCCCAGGCTCGTCCCCGGCCGGCAGCACCAAGCCTTTTGTGCACGCCGTG
CCCCCCTCTGACCCCCTGCGCCAGGCCAACCGCCTGCCAATCAAGGTGCTGAAGATGCTG
ACGGCACGAACTGGCCACATTTTGCACCCCGAGTACCTGCAGCCCCTGCCTTCCACGCCG
GTCAGCCCCATCGAGCTCGATGCCAAGAAGAGCCCGCTGGCGCTGTTGGCGCAAACATGT
TCGCAGATCGGGAAGCCCGACCCCTCGCCCTCCTCCAAACTCTCCTCGGTTGCCTCCAAC
GGGGGCGGCGCGGGCGGTGCCGGCGGCGGTGCTGCGGGCGACAAGGACACCAAATCGGGC
CCCCTGAAGCTGAGCGACATCGGCGTGGAGGACAAGTCGAGTTTCAAGCCGTACTCCAAA
CCCGGCTCGGATAAGAAGGAGCCGGGAGGCGGCGGTGGAGGCGGTGGCGGTGGCGGGGGC
GGCGGCGGGGGTGTTTCGTCGGAGAAGTCGGGATTCCGGGTACCGAGCGCCACCTGCCAG
CCATTCACGCCCAGGACAGGCAGCCCGAGCTCCAGCGCCTCGGCCTGCTCGCCGGGAGGT
ATGCTGTCCTCGGCCGGGGGTGCCCCGGAGGGCAAGGACGACAAGAAAGACACCGACGTG
GGCGGCGGTGGCAAGGGCACCGGGGGCGCCTCGGCCGAAGGGGGACCCACGGGGCTGGCA
CACGGCCGGATTAGCTGCGGCGGCGGGATTAATGTGGATGTGAACCAGCATCCGGATGGG
GGCCCGGGAGGCAAGGCTCTGGGCTCGGACTGCGGCGGTTCATCGGGCTCCAGCTCCGGC
TCCGGCCCCAGCGCGCCCACCTCCTCCTCAGTGTTGGGCTCTGGGCTGGTGGCTCCCGTG
TCACCCTACAAGCCGGGCCAGACAGTGTTCCCTCTGCCTCCCGCGGGTATGACCTACCCA
GGCAGCCTGGCCGGGGCCTACGCCGGCTACCCGCCCCAGTTCCTGCCACACGGCGTGGCA
CTTGACCCCACCAAGCCGGGCAGCCTGGTGGGGGCGCAGCTGGCGGCGGCCGCGGCCGGG
TCTCTGGGCTGCAGTAAGCCGGCCGGCTCCAGTCCTTTGGCCGGAGCGTCTCCGCCGTCC
GTGATGACAGCCAGTTTGTGCCGGGACCCTTACTGCCTCAGCTACCACTGCGCTAGCCAC
CTGGCAGGGGCGGCGGCCGCCAGCGCTTCTTGCGCACATGATCCGGCTGCTGCGGCTGCG
GCGCTGAAGTCCGGATACCCGCTGGTGTACCCCACGCACCCGCTGCACGGTGTGCACTCC
TCGCTAACGGCCGCCGCGGCTGCTGGCGCCACACCGCCCTCCCTGGCCGGCCACCCCCTC
TACCCCTACGGCTTTATGCTCCCTAACGACCCACTCCCCCACATCTGCAACTGGGTGTCG
GCCAACGGGCCGTGCGACAAGCGCTTCGCCACGTCCGAAGAGCTGCTGAGCCACTTGCGG
ACCCATACGGCATTTCCCGGGACAGACAAACTGCTGTCGGGCTACCCCAGCTCGTCGTCT
CTGGCCAGCGCTGCCGCGGCCGCCATGGCTTGCCACATGCACATCCCCACCTCGGGCGCA
CCGGGCAGCCCTGGGACGCTGGCGCTGCGCAGCCCCCACCACGCGCTGGGACTCAGCAGC
CGCTACCACCCCTACTCCAAGAGCCCGCTTCCCACGCCTGGCGCCCCCGTGCCGGTGCCC
GCCGCCACCGGACCGTACTACTCCCCCTACGCCCTCTACGGACAGAGACTGACCACCGCC
TCGGCGCTGGGGTATCAGTTG


Coding Sequence Details
Insert Sequence Verified?: N Verification Method: Insert was fully sequenced in parent vector
Reference Sequence Annotations:
End on reference sequence 2198
Start on reference sequence 258
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
5p GTACAAAAAAGCAGGCTCCACC(atg-goi)

3' Linker Sequence:
5p (goi-stop)GACCCAGCTTTCTTGTAC

 

 Recommended Growth Condition:

Distributed in bacterial strain : DH5-alpha T1 phage resistant
Antibiotic Selection: Host Type:  bacterial    Marker:  ampicillin
Host Type:  mammalian    Marker:  blasticidin
Bacterial Selection Condition:  100 ug/mL ampicillin
Growth Condition:  Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:  Commonly used conditions for ampicillin resistant plasmid clones.
Protein Expression Results: None
Recommended expression in: Not Applicable
       DyNA Vector Map

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Vector Name: pLenti6.2/V5-DEST

pLenti6.2V5-DEST in advanced viewed

Synonyms: pLenti6.2/V5-DEST
Sequencing Primer: Forward:  CMV forward
Reverse:  V5 reverse
Description: Lentiviral mammalian expression vector;CMV promoter and C-terminal V5 tag; ampicillin resistance in bacteria; blasticidin resistance in mammalian cells; Gateway recombinational cloning.
Comments: None
Size (bp): 8813
Parent Vector: None
Empty Vector: None
Properties: Gateway, acceptor (destination), lentiviral infection, mammalian expression, recombinational cloning, with tag/fusion/marker
Author Name: Invitrogen
Publications: None
Vector Map:         Vector Sequence:
Sequence:

 



Vector Features:

Type Name Description Start Position End Position
bacterial origin pUC ori pUC origin of replication 7733 8406
death cassette ccdB ccdB death cassette (removed when insert is present) 3767 3976
packaging signal HIV-1 psi HIV-1 psi packaging signal 521 565
poly-A signal SV40 SV40 polyadenylation signal 5639 5770
promoter RSV RSV/5' LTR hybrid promoter 1 410
promoter CMV CMV promoter driving gene of interest 1809 2392
promoter PGK PGK promoter to drive selection marker in mammalian cells 4266 4771
recombination site attR2 attR2 recombinational cloning site 4127 4142
recombination site attR1 attR1 recombinational cloning site 2440 2456
response element HIV-1 RRE HIV-1 Rev response element (RRE) 1075 1308
selectable marker AmpR Ampicillin resistance gene for selection in bacteria 6728 7588
selectable marker BlastR Blasticidin resistance gene in mammalian cells 4849 5249
selectable marker CmR chloramphenicol resistance marker (removed in plasmids with insert) 2673 3329
splice acceptor site 3' splice acceptor two 3' splice acceptors at 1656 and 1684 1656 1656
splice donor site 5' splice donor 5' splice donor 520 520
tag V5 C-terminal V5 epitope tag 4197 4238

 

  • Gene
  • Protein
  • Clone
  • Organism
  • Genome
  • Reagents

Gene

 

Gene Symbol : ZNF503
Symbol Nomenclature : ZNF503
SYNONYM : NOLZ-1; NOLZ1
Full Nomenclature : zinc finger protein 503
GENEID : 84858
HGNC : 23589
MIM : 613902
Vega : OTTHUMG00000018526
Target GenBank: BC011625

 

Reference Sequence Alignment

  

HsCD00329729      ATGAGCACAGCGCCCTCGCTTTCTGCCCTAAGAAGCAGTAAGCACAGCGGCGGCGGCGGC
NM_032772.4       ATGAGCACAGCGCCCTCGCTTTCTGCCCTAAGAAGCAGTAAGCACAGCGGCGGCGGCGGC
                  ************************************************************

HsCD00329729      GGCGGAGGCGGAGGCGGCGGTGCAGACCCTGCCTGGACCAGCGCGCTCTCTGGAAATAGC
NM_032772.4       GGCGGAGGCGGAGGCGGCGGTGCAGACCCTGCCTGGACCAGCGCGCTCTCTGGAAATAGC
                  ************************************************************

HsCD00329729      TCCGGCCCCGGCCCAGGCTCGTCCCCGGCCGGCAGCACCAAGCCTTTTGTGCACGCCGTG
NM_032772.4       TCCGGCCCCGGCCCAGGCTCGTCCCCGGCCGGCAGCACCAAGCCTTTTGTGCACGCCGTG
                  ************************************************************

HsCD00329729      CCCCCCTCTGACCCCCTGCGCCAGGCCAACCGCCTGCCAATCAAGGTGCTGAAGATGCTG
NM_032772.4       CCCCCCTCTGACCCCCTGCGCCAGGCCAACCGCCTGCCAATCAAGGTGCTGAAGATGCTG
                  ************************************************************

HsCD00329729      ACGGCACGAACTGGCCACATTTTGCACCCCGAGTACCTGCAGCCCCTGCCTTCCACGCCG
NM_032772.4       ACGGCACGAACTGGCCACATTTTGCACCCCGAGTACCTGCAGCCCCTGCCTTCCACGCCG
                  ************************************************************

HsCD00329729      GTCAGCCCCATCGAGCTCGATGCCAAGAAGAGCCCGCTGGCGCTGTTGGCGCAAACATGT
NM_032772.4       GTCAGCCCCATCGAGCTCGATGCCAAGAAGAGCCCGCTGGCGCTGTTGGCGCAAACATGT
                  ************************************************************

HsCD00329729      TCGCAGATCGGGAAGCCCGACCCCTCGCCCTCCTCCAAACTCTCCTCGGTTGCCTCCAAC
NM_032772.4       TCGCAGATCGGGAAGCCCGACCCCTCGCCCTCCTCCAAACTCTCCTCGGTTGCCTCCAAC
                  ************************************************************

HsCD00329729      GGGGGCGGCGCGGGCGGTGCCGGCGGCGGTGCTGCGGGCGACAAGGACACCAAATCGGGC
NM_032772.4       GGGGGCGGCGCGGGCGGTGCCGGCGGCGGTGCTGCGGGCGACAAGGACACCAAATCGGGC
                  ************************************************************

HsCD00329729      CCCCTGAAGCTGAGCGACATCGGCGTGGAGGACAAGTCGAGTTTCAAGCCGTACTCCAAA
NM_032772.4       CCCCTGAAGCTGAGCGACATCGGCGTGGAGGACAAGTCGAGTTTCAAGCCGTACTCCAAA
                  ************************************************************

HsCD00329729      CCCGGCTCGGATAAGAAGGAGCCGGGAGGCGGCGGTGGAGGCGGTGGCGGTGGCGGGGGC
NM_032772.4       CCCGGCTCGGATAAGAAGGAGCCGGGAGGCGGCGGTGGAGGCGGTGGCGGTGGCGGGGGC
                  ************************************************************

HsCD00329729      GGCGGCGGGGGTGTTTCGTCGGAGAAGTCGGGATTCCGGGTACCGAGCGCCACCTGCCAG
NM_032772.4       GGCGGCGGGGGTGTTTCGTCGGAGAAGTCGGGATTCCGGGTACCGAGCGCCACCTGCCAG
                  ************************************************************

HsCD00329729      CCATTCACGCCCAGGACAGGCAGCCCGAGCTCCAGCGCCTCGGCCTGCTCGCCGGGAGGT
NM_032772.4       CCATTCACGCCCAGGACAGGCAGCCCGAGCTCCAGCGCCTCGGCCTGCTCGCCGGGAGGT
                  ************************************************************

HsCD00329729      ATGCTGTCCTCGGCCGGGGGTGCCCCGGAGGGCAAGGACGACAAGAAAGACACCGACGTG
NM_032772.4       ATGCTGTCCTCGGCCGGGGGTGCCCCGGAGGGCAAGGACGACAAGAAAGACACCGACGTG
                  ************************************************************

HsCD00329729      GGCGGCGGTGGCAAGGGCACCGGGGGCGCCTCGGCCGAAGGGGGACCCACGGGGCTGGCA
NM_032772.4       GGCGGCGGTGGCAAGGGCACCGGGGGCGCCTCGGCCGAAGGGGGACCCACGGGGCTGGCA
                  ************************************************************

HsCD00329729      CACGGCCGGATTAGCTGCGGCGGCGGGATTAATGTGGATGTGAACCAGCATCCGGATGGG
NM_032772.4       CACGGCCGGATTAGCTGCGGCGGCGGGATTAATGTGGATGTGAACCAGCATCCGGATGGG
                  ************************************************************

HsCD00329729      GGCCCGGGAGGCAAGGCTCTGGGCTCGGACTGCGGCGGTTCATCGGGCTCCAGCTCCGGC
NM_032772.4       GGCCCGGGAGGCAAGGCTCTGGGCTCGGACTGCGGCGGTTCATCGGGCTCCAGCTCCGGC
                  ************************************************************

HsCD00329729      TCCGGCCCCAGCGCGCCCACCTCCTCCTCAGTGTTGGGCTCTGGGCTGGTGGCTCCCGTG
NM_032772.4       TCCGGCCCCAGCGCGCCCACCTCCTCCTCAGTGTTGGGCTCTGGGCTGGTGGCTCCCGTG
                  ************************************************************

HsCD00329729      TCACCCTACAAGCCGGGCCAGACAGTGTTCCCTCTGCCTCCCGCGGGTATGACCTACCCA
NM_032772.4       TCACCCTACAAGCCGGGCCAGACAGTGTTCCCTCTGCCTCCCGCGGGTATGACCTACCCA
                  ************************************************************

HsCD00329729      GGCAGCCTGGCCGGGGCCTACGCCGGCTACCCGCCCCAGTTCCTGCCACACGGCGTGGCA
NM_032772.4       GGCAGCCTGGCCGGGGCCTACGCCGGCTACCCGCCCCAGTTCCTGCCACACGGCGTGGCA
                  ************************************************************

HsCD00329729      CTTGACCCCACCAAGCCGGGCAGCCTGGTGGGGGCGCAGCTGGCGGCGGCCGCGGCCGGG
NM_032772.4       CTTGACCCCACCAAGCCGGGCAGCCTGGTGGGGGCGCAGCTGGCGGCGGCCGCGGCCGGG
                  ************************************************************

HsCD00329729      TCTCTGGGCTGCAGTAAGCCGGCCGGCTCCAGTCCTTTGGCCGGAGCGTCTCCGCCGTCC
NM_032772.4       TCTCTGGGCTGCAGTAAGCCGGCCGGCTCCAGCCCTTTGGCCGGAGCGTCTCCGCCGTCC
                  ******************************** ***************************

HsCD00329729      GTGATGACAGCCAGTTTGTGCCGGGACCCTTACTGCCTCAGCTACCACTGCGCTAGCCAC
NM_032772.4       GTGATGACAGCCAGTTTGTGCCGGGACCCTTACTGCCTCAGCTACCACTGCGCTAGCCAC
                  ************************************************************

HsCD00329729      CTGGCAGGGGCGGCGGCCGCCAGCGCTTCTTGCGCACATGATCCGGCTGCTGCGGCTGCG
NM_032772.4       CTGGCAGGGGCGGCGGCCGCCAGCGCTTCTTGCGCACATGATCCGGCTGCTGCGGCTGCG
                  ************************************************************

HsCD00329729      GCGCTGAAGTCCGGATACCCGCTGGTGTACCCCACGCACCCGCTGCACGGTGTGCACTCC
NM_032772.4       GCGCTGAAGTCCGGATACCCGCTGGTGTACCCCACGCACCCGCTGCACGGTGTGCACTCC
                  ************************************************************

HsCD00329729      TCGCTAACGGCCGCCGCGGCTGCTGGCGCCACACCGCCCTCCCTGGCCGGCCACCCCCTC
NM_032772.4       TCGCTAACGGCCGCCGCGGCTGCTGGCGCCACACCGCCCTCCCTGGCCGGCCACCCCCTC
                  ************************************************************

HsCD00329729      TACCCCTACGGCTTTATGCTCCCTAACGACCCACTCCCCCACATCTGCAACTGGGTGTCG
NM_032772.4       TACCCCTACGGCTTTATGCTCCCTAACGACCCACTCCCCCACATCTGCAACTGGGTGTCG
                  ************************************************************

HsCD00329729      GCCAACGGGCCGTGCGACAAGCGCTTCGCCACGTCCGAAGAGCTGCTGAGCCACTTGCGG
NM_032772.4       GCCAACGGGCCGTGCGACAAGCGCTTCGCCACGTCCGAAGAGCTGCTGAGCCACTTGCGG
                  ************************************************************

HsCD00329729      ACCCATACGGCATTTCCCGGGACAGACAAACTGCTGTCGGGCTACCCCAGCTCGTCGTCT
NM_032772.4       ACCCATACGGCATTTCCCGGGACAGACAAACTGCTGTCGGGCTACCCCAGCTCGTCGTCT
                  ************************************************************

HsCD00329729      CTGGCCAGCGCTGCCGCGGCCGCCATGGCTTGCCACATGCACATCCCCACCTCGGGCGCA
NM_032772.4       CTGGCCAGCGCTGCCGCGGCCGCCATGGCTTGCCACATGCACATCCCCACCTCGGGCGCA
                  ************************************************************

HsCD00329729      CCGGGCAGCCCTGGGACGCTGGCGCTGCGCAGCCCCCACCACGCGCTGGGACTCAGCAGC
NM_032772.4       CCGGGCAGCCCTGGGACGCTGGCGCTGCGCAGCCCCCACCACGCGCTGGGACTCAGCAGC
                  ************************************************************

HsCD00329729      CGCTACCACCCCTACTCCAAGAGCCCGCTTCCCACGCCTGGCGCCCCCGTGCCGGTGCCC
NM_032772.4       CGCTACCACCCCTACTCCAAGAGCCCGCTTCCCACGCCTGGCGCCCCCGTGCCGGTGCCC
                  ************************************************************

HsCD00329729      GCCGCCACCGGACCGTACTACTCCCCCTACGCCCTCTACGGACAGAGACTGACCACCGCC
NM_032772.4       GCCGCCACCGGACCGTACTACTCCCCCTACGCCCTCTACGGACAGAGACTGACCACCGCC
                  ************************************************************

HsCD00329729      TCGGCGCTGGGGTATCAGTTG
NM_032772.4       TCGGCGCTGGGGTATCAGTGA
                  ******************* .

Protein

UniProt : Q96F45
HPRD : 15811

Clone

Cloning Information : 268464
HIP Master Clone ID : 193492
Original Clone ID : FLH268464.01L

Organism


TAX_ID : 9606
Species Specific ID: 84858

Genome

Chromosome : 10
Map Location : 10q22.2
Ensembl : ENSG00000165655

Reagents

Labome : ZNF503-antibody