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NFYA (Homo sapiens) in pLenti6.2/V5-DEST (V5-tagged lentiviral mammalian expression vector)

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Explanation of Terms


Gene: Gene Symbol:  NFYA
Gene Name:  nuclear transcription factor Y, alpha
Sequence             
Original Clone ID: FLH268465.01L
Keyword: None
Species: Homo sapiens
Type: cDNA
Vector Name: pLenti6.2/V5-DEST               Format:  FUSION
Source: HIP
Description: None
Comments: None
Mutation/
Discrepancy :
No / No
Publications: None
Authors:
HsCD00329730

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AVAILABLE
No restriction
Special MTA: None

 

Insert sequence: 957nts         Open reading frame : 1 to 957
Reference Sequence Alignment

ATGGAGCAGTATACAGCAAACAGCAATAGTTCGACAGAGCAGATTGTTGTCCAGGCAGGA
CAGATTCAGCAGCAGGTCCAAGGGCAGCCATTAATGGTGCAGGTCAGTGGAGGCCAGCTA
ATCACATCAACTGGCCAACCCATCATGGTCCAGGCTGTCCCTGGTGGGCAAGGTCAAACC
ATCATGCAAGTACCTGTTTCTGGAACACAGGGTTTGCAGCAAATACAGTTGGTCCCACCT
GGACAGATCCAGATCCAGGGTGGACAGGCTGTGCAGGTGCAGGGCCAGCAGGGCCAGACC
CAGCAGATCATCATCCAGCAGCCCCAGACGGCTGTCACTGCTGGCCAGACTCAGACACAG
CAGCAGATTGCTGTCCAGGGACAGCAAGTGGCACAGACTGCTGAAGGGCAGACCATCGTC
TATCAACCAGTTAATGCAGATGGCACCATTCTCCAGCAAGTTACAGTCCCTGTTTCAGGC
ATGATCACTATCCCAGCAGCCAGTTTGGCAGGAGCACAGATTGTTCAAACAGGAGCCAAT
ACCAACACAACCAGCAGTGGGCAAGGGACTGTCACTGTGACACTACCAGTGGCAGGCAAT
GTGGTCAATTCAGGAGGGATGGTCATGATGGTTCCTGGGGCTGGCTCTGTGCCTGCTATC
CAAAGAATCCCTCTACCTGGAGCAGAGATGCTTGAAGAAGAGCCTCTCTACGTGAATGCC
AAACAATACAACCGTATTCTTAAGAGGAGGCAAGCCCGAGCTAAACTAGAGGCAGAAGGG
AAAATTCCAAAGGAGAGAAGGAAATACCTGCATGAGTCTCGGCACCGTCATGCCATGGCA
CGGAAGCGTGGTGAAGGTGGACGATTTTTCTCTCCAAAGGAAAAGGATAGTCCCCATATG
CAGGATCCAAACCAAGCCGATGAAGAAGCAATGACACAGATCATCCGAGTGTCCTTG


Coding Sequence Details
Insert Sequence Verified?: N Verification Method: Insert was fully sequenced in parent vector
Reference Sequence Annotations:
End on reference sequence 1388
Start on reference sequence 432
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
5p GTACAAAAAAGCAGGCTCCACC(atg-goi)

3' Linker Sequence:
5p (goi-stop)GACCCAGCTTTCTTGTAC

 

 Recommended Growth Condition:

Distributed in bacterial strain : DH5-alpha T1 phage resistant
Antibiotic Selection: Host Type:  bacterial    Marker:  ampicillin
Host Type:  mammalian    Marker:  blasticidin
Bacterial Selection Condition:  100 ug/mL ampicillin
Growth Condition:  Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:  Commonly used conditions for ampicillin resistant plasmid clones.
Protein Expression Results: None
Recommended expression in: Not Applicable
       DyNA Vector Map

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Vector Name: pLenti6.2/V5-DEST

pLenti6.2V5-DEST in advanced viewed

Synonyms: pLenti6.2/V5-DEST
Sequencing Primer: Forward:  CMV forward
Reverse:  V5 reverse
Description: Lentiviral mammalian expression vector;CMV promoter and C-terminal V5 tag; ampicillin resistance in bacteria; blasticidin resistance in mammalian cells; Gateway recombinational cloning.
Comments: None
Size (bp): 8813
Parent Vector: None
Empty Vector: None
Properties: Gateway, acceptor (destination), lentiviral infection, mammalian expression, recombinational cloning, with tag/fusion/marker
Author Name: Invitrogen
Publications: None
Vector Map:         Vector Sequence:
Sequence:

 



Vector Features:

Type Name Description Start Position End Position
bacterial origin pUC ori pUC origin of replication 7733 8406
death cassette ccdB ccdB death cassette (removed when insert is present) 3767 3976
packaging signal HIV-1 psi HIV-1 psi packaging signal 521 565
poly-A signal SV40 SV40 polyadenylation signal 5639 5770
promoter RSV RSV/5' LTR hybrid promoter 1 410
promoter CMV CMV promoter driving gene of interest 1809 2392
promoter PGK PGK promoter to drive selection marker in mammalian cells 4266 4771
recombination site attR2 attR2 recombinational cloning site 4127 4142
recombination site attR1 attR1 recombinational cloning site 2440 2456
response element HIV-1 RRE HIV-1 Rev response element (RRE) 1075 1308
selectable marker AmpR Ampicillin resistance gene for selection in bacteria 6728 7588
selectable marker BlastR Blasticidin resistance gene in mammalian cells 4849 5249
selectable marker CmR chloramphenicol resistance marker (removed in plasmids with insert) 2673 3329
splice acceptor site 3' splice acceptor two 3' splice acceptors at 1656 and 1684 1656 1656
splice donor site 5' splice donor 5' splice donor 520 520
tag V5 C-terminal V5 epitope tag 4197 4238

 

  • Gene
  • Pathways
  • Protein
  • Clone
  • Organism
  • Genome
  • Reagents

Gene

 

Gene Symbol : NFYA
Symbol Nomenclature : NFYA
SYNONYM : CBF-A; CBF-B; HAP2; NF-YA
Designation : CAAT box DNA-binding protein subunit A|CAAT-box DNA binding protein subunit A|CCAAT-binding transcription factor subunit B|HAP2 CCAAT-binding protein|Transcription factor NF-Y, A subunit|nuclear transcription factor Y subunit A|nuclear transcription factor Y subunit alpha
Full Nomenclature : nuclear transcription factor Y, alpha
GENEID : 4800
HGNC : 7804
MIM : 189903
Target GenBank: BC039244

 

Reference Sequence Alignment

  

HsCD00329730      ATGGAGCAGTATACAGCAAACAGCAATAGTTCGACAGAGCAGATTGTTGTCCAGGCAGGA
NM_002505.4       ATGGAGCAGTATACAGCAAACAGCAATAGTTCGACAGAGCAGATTGTTGTCCAGGCAGGA
NM_021705.3       ATGGAGCAGTATACAGCAAACAGCAATAGTTCGACAGAGCAGATTGTTGTCCAGGCAGGA
                  ************************************************************

HsCD00329730      CAGATTCAGCAGCAGGTCCAAG--------------------------------------
NM_002505.4       CAGATTCAGCAGCAG----CAGCAGGGTGGTGTCACTGCTGTGCAGTTGCAGACTGAGGC
NM_021705.3       CAGATTCAGCAGCAG----GTCCAAG----------------------------------
                  ***************     :                                       

HsCD00329730      -----------------------------------------------------GGCAGCC
NM_002505.4       CCAGGTGGCATCCGCCTCAGGCCAGCAAGTCCAGACCCTCCAGGTAGTCCAAGGGCAGCC
NM_021705.3       -----------------------------------------------------GGCAGCC
                                                                       *******

HsCD00329730      ATTAATGGTGCAGGTCAGTGGAGGCCAGCTAATCACATCAACTGGCCAACCCATCATGGT
NM_002505.4       ATTAATGGTGCAGGTCAGTGGAGGCCAGCTAATCACATCAACTGGCCAACCCATCATGGT
NM_021705.3       ATTAATGGTGCAGGTCAGTGGAGGCCAGCTAATCACATCAACTGGCCAACCCATCATGGT
                  ************************************************************

HsCD00329730      CCAGGCTGTCCCTGGTGGGCAAGGTCAAACCATCATGCAAGTACCTGTTTCTGGAACACA
NM_002505.4       CCAGGCTGTCCCTGGTGGACAAGGTCAAACCATCATGCAAGTACCTGTTTCTGGAACACA
NM_021705.3       CCAGGCTGTCCCTGGTGGACAAGGTCAAACCATCATGCAAGTACCTGTTTCTGGAACACA
                  ******************.*****************************************

HsCD00329730      GGGTTTGCAGCAAATACAGTTGGTCCCACCTGGACAGATCCAGATCCAGGGTGGACAGGC
NM_002505.4       GGGTTTGCAGCAAATACAGTTGGTCCCACCTGGACAGATCCAGATCCAGGGTGGACAGGC
NM_021705.3       GGGTTTGCAGCAAATACAGTTGGTCCCACCTGGACAGATCCAGATCCAGGGTGGACAGGC
                  ************************************************************

HsCD00329730      TGTGCAGGTGCAGGGCCAGCAGGGCCAGACCCAGCAGATCATCATCCAGCAGCCCCAGAC
NM_002505.4       TGTGCAGGTGCAGGGCCAGCAGGGCCAGACCCAGCAGATCATCATCCAGCAGCCCCAGAC
NM_021705.3       TGTGCAGGTGCAGGGCCAGCAGGGCCAGACCCAGCAGATCATCATCCAGCAGCCCCAGAC
                  ************************************************************

HsCD00329730      GGCTGTCACTGCTGGCCAGACTCAGACACAGCAGCAGATTGCTGTCCAGGGACAGCAAGT
NM_002505.4       GGCTGTCACTGCTGGCCAGACTCAGACACAGCAGCAGATTGCTGTCCAGGGACAGCAAGT
NM_021705.3       GGCTGTCACTGCTGGCCAGACTCAGACACAGCAGCAGATTGCTGTCCAGGGACAGCAAGT
                  ************************************************************

HsCD00329730      GGCACAGACTGCTGAAGGGCAGACCATCGTCTATCAACCAGTTAATGCAGATGGCACCAT
NM_002505.4       GGCACAGACTGCTGAAGGGCAGACCATCGTCTATCAACCAGTTAATGCAGATGGCACCAT
NM_021705.3       GGCACAGACTGCTGAAGGGCAGACCATCGTCTATCAACCAGTTAATGCAGATGGCACCAT
                  ************************************************************

HsCD00329730      TCTCCAGCAAGTTACAGTCCCTGTTTCAGGCATGATCACTATCCCAGCAGCCAGTTTGGC
NM_002505.4       TCTCCAGCAAGTTACAGTCCCTGTTTCAGGCATGATCACTATCCCAGCAGCCAGTTTGGC
NM_021705.3       TCTCCAGCAAGTTACAGTCCCTGTTTCAGGCATGATCACTATCCCAGCAGCCAGTTTGGC
                  ************************************************************

HsCD00329730      AGGAGCACAGATTGTTCAAACAGGAGCCAATACCAACACAACCAGCAGTGGGCAAGGGAC
NM_002505.4       AGGAGCACAGATTGTTCAAACAGGAGCCAATACCAACACAACCAGCAGTGGGCAAGGGAC
NM_021705.3       AGGAGCACAGATTGTTCAAACAGGAGCCAATACCAACACAACCAGCAGTGGGCAAGGGAC
                  ************************************************************

HsCD00329730      TGTCACTGTGACACTACCAGTGGCAGGCAATGTGGTCAATTCAGGAGGGATGGTCATGAT
NM_002505.4       TGTCACTGTGACACTACCAGTGGCAGGCAATGTGGTCAATTCAGGAGGGATGGTCATGAT
NM_021705.3       TGTCACTGTGACACTACCAGTGGCAGGCAATGTGGTCAATTCAGGAGGGATGGTCATGAT
                  ************************************************************

HsCD00329730      GGTTCCTGGGGCTGGCTCTGTGCCTGCTATCCAAAGAATCCCTCTACCTGGAGCAGAGAT
NM_002505.4       GGTTCCTGGGGCTGGCTCTGTGCCTGCTATCCAAAGAATCCCTCTACCTGGAGCAGAGAT
NM_021705.3       GGTTCCTGGGGCTGGCTCTGTGCCTGCTATCCAAAGAATCCCTCTACCTGGAGCAGAGAT
                  ************************************************************

HsCD00329730      GCTTGAAGAAGAGCCTCTCTACGTGAATGCCAAACAATACAACCGTATTCTTAAGAGGAG
NM_002505.4       GCTTGAAGAAGAGCCTCTCTACGTGAATGCCAAACAATACCACCGTATTCTTAAGAGGAG
NM_021705.3       GCTTGAAGAAGAGCCTCTCTACGTGAATGCCAAACAATACCACCGTATTCTTAAGAGGAG
                  ****************************************.*******************

HsCD00329730      GCAAGCCCGAGCTAAACTAGAGGCAGAAGGGAAAATTCCAAAGGAGAGAAGGAAATACCT
NM_002505.4       GCAAGCCCGAGCTAAACTAGAGGCAGAAGGGAAAATTCCAAAGGAGAGAAGGAAATACCT
NM_021705.3       GCAAGCCCGAGCTAAACTAGAGGCAGAAGGGAAAATTCCAAAGGAGAGAAGGAAATACCT
                  ************************************************************

HsCD00329730      GCATGAGTCTCGGCACCGTCATGCCATGGCACGGAAGCGTGGTGAAGGTGGACGATTTTT
NM_002505.4       GCATGAGTCTCGGCACCGTCATGCCATGGCACGGAAGCGTGGTGAAGGTGGACGATTTTT
NM_021705.3       GCATGAGTCTCGGCACCGTCATGCCATGGCACGGAAGCGTGGTGAAGGTGGACGATTTTT
                  ************************************************************

HsCD00329730      CTCTCCAAAGGAAAAGGATAGTCCCCATATGCAGGATCCAAACCAAGCCGATGAAGAAGC
NM_002505.4       CTCTCCAAAGGAAAAGGATAGTCCCCATATGCAGGATCCAAACCAAGCCGATGAAGAAGC
NM_021705.3       CTCTCCAAAGGAAAAGGATAGTCCCCATATGCAGGATCCAAACCAAGCCGATGAAGAAGC
                  ************************************************************

HsCD00329730      AATGACACAGATCATCCGAGTGTCCTTG
NM_002505.4       AATGACACAGATCATCCGAGTGTCCTAA
NM_021705.3       AATGACACAGATCATCCGAGTGTCCTAA
                  **************************:.

Pathways

NCI : Direct p53 effectors
NCI : Validated targets of C-MYC transcriptional repression
Reactome : ATF4 activates genes
Reactome : ATF6-alpha activates chaperone genes
Reactome : ATF6-alpha activates chaperones
Reactome : Activation of gene expression by SREBF (SREBP)
Reactome : Fatty acid, triacylglycerol, and ketone body metabolism
Reactome : Metabolism
Reactome : Metabolism of lipids and lipoproteins
Reactome : Metabolism of proteins
Reactome : PERK regulates gene expression
Reactome : PPARA activates gene expression
Reactome : Regulation of cholesterol biosynthesis by SREBP (SREBF)
Reactome : Regulation of lipid metabolism by Peroxisome proliferator-activated receptor alpha (PPARalpha)
Reactome : Unfolded Protein Response (UPR)
WikiPathway : SIDS Susceptibility Pathways
WikiPathway : SREBP signalling
WikiPathway : Wnt Signaling Pathway and Pluripotency

Protein

SMART domain : CBF : CCAAT-Binding transcription Factor
UniProt : P23511
HPRD : 01793

Clone

Cloning Information : 268465
HIP Master Clone ID : 193495
Original Clone ID : FLH268465.01L

Organism


TAX_ID : 9606
Species Specific ID: 4800

Genome

Chromosome : 6
Map Location : 6p21.3

Reagents

Labome : NF-YA-antibody