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HSF2BP (Homo sapiens) in pLenti6.2/V5-DEST (V5-tagged lentiviral mammalian expression vector)

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Explanation of Terms


Gene: Gene Symbol:  HSF2BP
Gene Name:  heat shock transcription factor 2 binding protein
Sequence             
Original Clone ID: FLH268467.01L
Keyword: None
Species: Homo sapiens
Type: cDNA
Vector Name: pLenti6.2/V5-DEST               Format:  FUSION
Source: HIP
Description: None
Comments: None
Mutation/
Discrepancy :
No / No
Publications: None
Authors:
HsCD00329731

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AVAILABLE
No restriction
Special MTA: None

 

Insert sequence: 1005nts         Open reading frame : 1 to 1005
Reference Sequence Alignment

ATGGGCGAAGCGGGCGCCGCTGAGGAGGCCTGCCGGCACATGGGAACTAAAGAGGAATTT
GTTAAAGTCAGAAAGAAGGATCTGGAACGGCTGACAACTGAAGTGATGCAAATACGGGAC
TTCTTACCCAGAATACTAAATGGGGAGGTGCTGGAGAGCTTCCAGAAATTAAAGATTGTA
GAAAAAAACCTGGAAAGGAAAGAGCAAGAATTAGAGCAGCTGAAAATGGATTGTGAGCAC
TTTAAAGCCCGCCTGGAAACCGTGCAGGCCGACAACATAAGAGAGAAGAAGGAGAAACTG
GCTCTTCGACAGCAGTTGAATGAAGCGAAGCAGCAACTCCTGCAGCAGGCAGAGTATTGT
ACAGAAATGGGAGCAGCAGCGTGTACCCTCTTGTGGGGTGTCTCCAGCAGTGAGGAAGTC
GTCAAGGCCATTTTGGGAGGAGATAAAGCTTTGAAGTTTTTCAGCATCACTGGTCAAACA
ATGGAGAGTTTTGTGAAGTCGTTAGACGGTGATGTCCAGGAGCTGGATTCGGATGAAAGT
CAGTTTGTTTTCGCTCTGGCTGGAATTGTCACGAATGTTGCTGCTATAGCATGTGGTCGT
GAATTCTTGGTTAATTCAAGCCGGGTGCTCTTGGACACCATATTGCAGCTTCTGGGAGAC
TTGAAGCCAGGACAGTGTACCAAACTCAAAGTGCTAATGCTGATGTCCCTATACAATGTA
AGCATCAATTTGAAAGGCTTGAAATACATCAGCGAGAGTCCAGGATTCATCCCTTTGCTG
TGGTGGCTTTTGAGTGATCCAGATGCAGAGGTGTGCCTTCATGTACTGAGGCTTGTCCAG
TCTGTGGTTCTGGAACCTGAAGTCTTCTCCAAGTCGGCCTCTGAGTTCCGGAGCTCCCTG
CCCCTGCAACGCATCCTGGCAATGTCCAAGAGCCGCAACCCCCGCCTGCAAACCGCAGCC
CAGGAGCTCCTGGAAGATCTCCGCACTCTGGAGCATAATGTGTTG


Coding Sequence Details
Insert Sequence Verified?: N Verification Method: Insert was fully sequenced in parent vector
Reference Sequence Annotations:
End on reference sequence 1320
Start on reference sequence 316
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
5p GTACAAAAAAGCAGGCTCCACC(atg-goi)

3' Linker Sequence:
5p (goi-stop)GACCCAGCTTTCTTGTAC

 

 Recommended Growth Condition:

Distributed in bacterial strain : DH5-alpha T1 phage resistant
Antibiotic Selection: Host Type:  bacterial    Marker:  ampicillin
Host Type:  mammalian    Marker:  blasticidin
Bacterial Selection Condition:  100 ug/mL ampicillin
Growth Condition:  Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:  Commonly used conditions for ampicillin resistant plasmid clones.
Protein Expression Results: None
Recommended expression in: Not Applicable
       DyNA Vector Map

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Vector Name: pLenti6.2/V5-DEST

pLenti6.2V5-DEST in advanced viewed

Synonyms: pLenti6.2/V5-DEST
Sequencing Primer: Forward:  CMV forward
Reverse:  V5 reverse
Description: Lentiviral mammalian expression vector;CMV promoter and C-terminal V5 tag; ampicillin resistance in bacteria; blasticidin resistance in mammalian cells; Gateway recombinational cloning.
Comments: None
Size (bp): 8813
Parent Vector: None
Empty Vector: None
Properties: Gateway, acceptor (destination), lentiviral infection, mammalian expression, recombinational cloning, with tag/fusion/marker
Author Name: Invitrogen
Publications: None
Vector Map:         Vector Sequence:
Sequence:

 



Vector Features:

Type Name Description Start Position End Position
bacterial origin pUC ori pUC origin of replication 7733 8406
death cassette ccdB ccdB death cassette (removed when insert is present) 3767 3976
packaging signal HIV-1 psi HIV-1 psi packaging signal 521 565
poly-A signal SV40 SV40 polyadenylation signal 5639 5770
promoter RSV RSV/5' LTR hybrid promoter 1 410
promoter CMV CMV promoter driving gene of interest 1809 2392
promoter PGK PGK promoter to drive selection marker in mammalian cells 4266 4771
recombination site attR2 attR2 recombinational cloning site 4127 4142
recombination site attR1 attR1 recombinational cloning site 2440 2456
response element HIV-1 RRE HIV-1 Rev response element (RRE) 1075 1308
selectable marker AmpR Ampicillin resistance gene for selection in bacteria 6728 7588
selectable marker BlastR Blasticidin resistance gene in mammalian cells 4849 5249
selectable marker CmR chloramphenicol resistance marker (removed in plasmids with insert) 2673 3329
splice acceptor site 3' splice acceptor two 3' splice acceptors at 1656 and 1684 1656 1656
splice donor site 5' splice donor 5' splice donor 520 520
tag V5 C-terminal V5 epitope tag 4197 4238

 

  • Gene
  • Protein
  • Clone
  • Organism
  • Genome
  • Reagents

Gene

 

Gene Symbol : HSF2BP
Symbol Nomenclature : HSF2BP
Designation : heat shock factor 2-binding protein
Full Nomenclature : heat shock transcription factor 2 binding protein
GENEID : 11077
HGNC : 5226
MIM : 604554
Vega : OTTHUMG00000086863
Target GenBank: BC000153

 

Reference Sequence Alignment

  

HsCD00329731        ATGGGCGAAGCGGGCGCCGCTGAGGAGGCCTGCCGGCACATGGGAACTAAAGAGGAATTT
NM_007031.1         ATGGGCGAAGCGGGCGCCGCTGAGGAGGCCTGCCGGCACATGGGAACTAAAGAGGAATTT
XM_005261090.1      ---------------------------------------ATGGGAACTAAAGAGGAATTT
                                                           *********************

HsCD00329731        GTTAAAGTCAGAAAGAAGGATCTGGAACGGCTGACAACTGAAGTGATGCAAATACGGGAC
NM_007031.1         GTTAAAGTCAGAAAGAAGGATCTGGAACGGCTGACAACTGAAGTGATGCAAATACGGGAC
XM_005261090.1      GTTAAAGTCAGAAAGAAGGATCTGGAACGGCTGACAACTGAAGTGATGCAAATACGGGAC
                    ************************************************************

HsCD00329731        TTCTTACCCAGAATACTAAATGGGGAGGTGCTGGAGAGCTTCCAGAAATTAAAGATTGTA
NM_007031.1         TTCTTACCCAGAATACTAAATGGGGAGGTGCTGGAGAGCTTCCAGAAATTAAAGATTGTA
XM_005261090.1      TTCTTACCCAGAATACTAAATGGGGAGGTGCTGGAGAGCTTCCAGAAATTAAAGATTGTA
                    ************************************************************

HsCD00329731        GAAAAAAACCTGGAAAGGAAAGAGCAAGAATTAGAGCAGCTGAAAATGGATTGTGAGCAC
NM_007031.1         GAAAAAAACCTGGAAAGGAAAGAGCAAGAATTAGAGCAGCTGAAAATGGATTGTGAGCAC
XM_005261090.1      GAAAAAAACCTGGAAAGGAAAGAGCAAGAATTAGAGCAGCTGAAAATGGATTGTGAGCAC
                    ************************************************************

HsCD00329731        TTTAAAGCCCGCCTGGAAACCGTGCAGGCCGACAACATAAGAGAGAAGAAGGAGAAACTG
NM_007031.1         TTTAAAGCCCGCCTGGAAACCGTGCAGGCCGACAACATAAGAGAGAAGAAGGAGAAACTG
XM_005261090.1      TTTAAAGCCCGCCTGGAAACCGTGCAGGCCGACAACATAAGAGAGAAGAAGGAGAAACTG
                    ************************************************************

HsCD00329731        GCTCTTCGACAGCAGTTGAATGAAGCGAAGCAGCAACTCCTGCAGCAGGCAGAGTATTGT
NM_007031.1         GCTCTTCGACAGCAGTTGAATGAAGCGAAGCAGCAACTCCTGCAGCAGGCAGAGTATTGT
XM_005261090.1      GCTCTTCGACAGCAGTTGAATGAAGCGAAGCAGCAACTCCTGCAGCAGGCAGAGTATTGT
                    ************************************************************

HsCD00329731        ACAGAAATGGGAGCAGCAGCGTGTACCCTCTTGTGGGGTGTCTCCAGCAGTGAGGAAGTC
NM_007031.1         ACAGAAATGGGAGCAGCAGCGTGTACCCTCTTGTGGGGTGTCTCCAGCAGTGAGGAAGTC
XM_005261090.1      ACAGAAATGGGAGCAGCAGCGTGTACCCTCTTGTGGGGTGTCTCCAGCAGTGAGGAAGTC
                    ************************************************************

HsCD00329731        GTCAAGGCCATTTTGGGAGGAGATAAAGCTTTGAAGTTTTTCAGCATCACTGGTCAAACA
NM_007031.1         GTCAAGGCCATTTTGGGAGGAGATAAAGCTTTGAAGTTTTTCAGCATCACTGGTCAAACA
XM_005261090.1      GTCAAGGCCATTTTGGGAGGAGATAAAGCTTTGAAGTTTTTCAGCATCACTGGTCAAACA
                    ************************************************************

HsCD00329731        ATGGAGAGTTTTGTGAAGTCGTTAGACGGTGATGTCCAGGAGCTGGATTCGGATGAAAGT
NM_007031.1         ATGGAGAGTTTTGTGAAGTCGTTAGACGGTGATGTCCAGGAGCTGGATTCGGATGAAAGT
XM_005261090.1      ATGGAGAGTTTTGTGAAGTCGTTAGACGGTGATGTCCAGGAGCTGGATTCGGATGAAAGT
                    ************************************************************

HsCD00329731        CAGTTTGTTTTCGCTCTGGCTGGAATTGTCACGAATGTTGCTGCTATAGCATGTGGTCGT
NM_007031.1         CAGTTTGTTTTCGCTCTGGCTGGAATTGTCACGAATGTTGCTGCTATAGCATGTGGTCGT
XM_005261090.1      CAGTTTGTTTTCGCTCTGGCTGGAATTGTCACGAATGTTGCTGCTATAGCATGTGGTCGT
                    ************************************************************

HsCD00329731        GAATTCTTGGTTAATTCAAGCCGGGTGCTCTTGGACACCATATTGCAGCTTCTGGGAGAC
NM_007031.1         GAATTCTTGGTTAATTCAAGCCGGGTGCTCTTGGACACCATATTGCAGCTTCTGGGAGAC
XM_005261090.1      GAATTCTTGGTTAATTCAAGCCGGGTGCTCTTGGACACCATATTGCAGCTTCTGGGAGAC
                    ************************************************************

HsCD00329731        TTGAAGCCAGGACAGTGTACCAAACTCAAAGTGCTAATGCTGATGTCCCTATACAATGTA
NM_007031.1         TTGAAGCCAGGACAGTGTACCAAACTCAAAGTGCTAATGCTGATGTCCCTATACAATGTA
XM_005261090.1      TTGAAGCCAGGACAGTGTACCAAACTCAAAGTGCTAATGCTGATGTCCCTATACAATGTA
                    ************************************************************

HsCD00329731        AGCATCAATTTGAAAGGCTTGAAATACATCAGCGAGAGTCCAGGATTCATCCCTTTGCTG
NM_007031.1         AGCATCAATTTGAAAGGCTTGAAATACATCAGCGAGAGTCCAGGATTCATCCCTTTGCTG
XM_005261090.1      AGCATCAATTTGAAAGGCTTGAAATACATCAGCGAGAGTCCAGGATTCATCCCTTTGCTG
                    ************************************************************

HsCD00329731        TGGTGGCTTTTGAGTGATCCAGATGCAGAGGTGTGCCTTCATGTACTGAGGCTTGTCCAG
NM_007031.1         TGGTGGCTTTTGAGTGATCCAGATGCAGAGGTGTGCCTTCATGTACTGAGGCTTGTCCAG
XM_005261090.1      TGGTGGCTTTTGAGTGATCCAGATGCAGAGGTGTGCCTTCATGTACTGAGGCTTGTCCAG
                    ************************************************************

HsCD00329731        TCTGTGGTTCTGGAACCTGAAGTCTTCTCCAAGTCGGCCTCTGAGTTCCGGAGCTCCCTG
NM_007031.1         TCTGTGGTTCTGGAACCTGAAGTCTTCTCCAAGTCGGCCTCTGAGTTCCGGAGCTCCCTG
XM_005261090.1      TCTGTGGTTCTGGAACCTGAAGTCTTCTCCAAGTCGGCCTCTGAGTTCCGGAGCTCCCTG
                    ************************************************************

HsCD00329731        CCCCTGCAACGCATCCTGGCAATGTCCAAGAGCCGCAACCCCCGCCTGCAAACCGCAGCC
NM_007031.1         CCCCTGCAACGCATCCTGGCAATGTCCAAGAGCCGCAACCCCCGCCTGCAAACCGCAGCC
XM_005261090.1      CCCCTGCAACGCATCCTGGCAATGTCCAAGAGCCGCAACCCCCGCCTGCAAACCGCAGCC
                    ************************************************************

HsCD00329731        CAGGAGCTCCTGGAAGATCTCCGCACTCTGGAGCATAATGTGTTG
NM_007031.1         CAGGAGCTCCTGGAAGATCTCCGCACTCTGGAGCATAATGTGTAG
XM_005261090.1      CAGGAGCTCCTGGAAGATCTCCGCACTCTGGAGCATAATGTGTAG
                    *******************************************:*

Protein

UniProt : O75031
UniProt : Q6IAT7
HPRD : 05186

Clone

Cloning Information : 268467
HIP Master Clone ID : 193501
Original Clone ID : FLH268467.01L

Organism


TAX_ID : 9606
Species Specific ID: 11077

Genome

Chromosome : 21
Map Location : 21q22.3
Ensembl : ENSG00000160207

Reagents

Labome : HSF2BP-antibody