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PAX4 (Homo sapiens) in pLenti6.2/V5-DEST (V5-tagged lentiviral mammalian expression vector)

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Explanation of Terms


Gene: Gene Symbol:  PAX4
Gene Name:  paired box 4
Sequence             
Original Clone ID: FLH268471.01L
Keyword: None
Species: Homo sapiens
Type: cDNA
Vector Name: pLenti6.2/V5-DEST               Format:  FUSION
Source: HIP
Description: None
Comments: None
Mutation/
Discrepancy :
No / No
Publications: None
Authors:
HsCD00329734

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AVAILABLE
No restriction
Special MTA: None

 

Insert sequence: 1032nts         Open reading frame : 1 to 1032
Reference Sequence Alignment

ATGAACCAGCTTGGGGGGCTCTTTGTGAATGGCCGGCCCCTGCCTCTGGATACCCGGCAG
CAGATTGTGCGGCTAGCAGTCAGTGGAATGCGGCCCTGTGACATCTCACGGATCCTTAAG
GTATCTAATGGCTGTGTGAGCAAGATCCTAGGGCGTTACTACCGCACAGGTGTCTTGGAG
CCAAAGGGCATTGGGGGAAGCAAGCCACGGCTGGCTACACCCCCTGTGGTGGCTCGAATT
GCCCAGCTGAAGGGTGAGTGTCCAGCCCTCTTTGCCTGGGAAATCCAACGCCAGCTTTGT
GCTGAAGGGCTTTGCACCCAGGACAAGACTCCCAGTGTCTCCTCCATCAACCGAGTCCTG
CGGGCATTACAGGAGGACCAGGGACTACCGTGCACACGGCTCAGGTCACCAGCTGTTTTG
GCTCCAGCTGTCCTCACTCCCCATAGTGGCTCTGAGACTCCCCGGGGTACCCACCCAGGG
ACCGGCCACCGGAATCGGACTATCTTCTCCCCAAGCCAGGCAGAGGCACTGGAGAAAGAG
TTCCAGCGTGGGCAGTATCCTGATTCAGTGGCCCGTGGAAAGCTGGCTACTGCCACCTCT
CTGCCTGAGGACACGGTGAGGGTCTGGTTTTCCAACAGAAGAGCCAAATGGCGTCGGCAA
GAGAAGCTCAAGTGGGAAATGCAGCTGCCAGGTGCTTCCCAGGGGCTGACTGTACCAAGG
GTTGCCCCAGGAATCATCTCTGCACAGCAGTCCCCTGGCAGTGTGCCCACAGCAGCCCTG
CCTGCCCTGGAACCACTGGGTCCCTCCTGCTATCAGCTGTGCTGGGCAACAGCACCAGAA
AGGTGTCTGAGTGACACCCCACCTAAAGCCTGTCTCAAGCCCTGCTGGGGCCACTTGCCC
CCACAGCCGAATTCCCTGGACTCAGGACTGCTTTGCCTTCCTTGCCCTTCCTCCCACTGT
CCCCTGGCCAGTCTTAGTGGCTCTCAGGCCCTGCTCTGGCCTGGCTGCCCACTACTGTAT
GGCTTGGAATTG


Coding Sequence Details
Insert Sequence Verified?: N Verification Method: Insert was fully sequenced in parent vector
Reference Sequence Annotations:
End on reference sequence 1065
Start on reference sequence 34
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
5p GTACAAAAAAGCAGGCTCCACC(atg-goi)

3' Linker Sequence:
5p (goi-stop)GACCCAGCTTTCTTGTAC

 

 Recommended Growth Condition:

Distributed in bacterial strain : DH5-alpha T1 phage resistant
Antibiotic Selection: Host Type:  bacterial    Marker:  ampicillin
Host Type:  mammalian    Marker:  blasticidin
Bacterial Selection Condition:  100 ug/mL ampicillin
Growth Condition:  Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:  Commonly used conditions for ampicillin resistant plasmid clones.
Protein Expression Results: None
Recommended expression in: Not Applicable
       DyNA Vector Map

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Vector Name: pLenti6.2/V5-DEST

pLenti6.2V5-DEST in advanced viewed

Synonyms: pLenti6.2/V5-DEST
Sequencing Primer: Forward:  CMV forward
Reverse:  V5 reverse
Description: Lentiviral mammalian expression vector;CMV promoter and C-terminal V5 tag; ampicillin resistance in bacteria; blasticidin resistance in mammalian cells; Gateway recombinational cloning.
Comments: None
Size (bp): 8813
Parent Vector: None
Empty Vector: None
Properties: Gateway, acceptor (destination), lentiviral infection, mammalian expression, recombinational cloning, with tag/fusion/marker
Author Name: Invitrogen
Publications: None
Vector Map:         Vector Sequence:
Sequence:

 



Vector Features:

Type Name Description Start Position End Position
bacterial origin pUC ori pUC origin of replication 7733 8406
death cassette ccdB ccdB death cassette (removed when insert is present) 3767 3976
packaging signal HIV-1 psi HIV-1 psi packaging signal 521 565
poly-A signal SV40 SV40 polyadenylation signal 5639 5770
promoter RSV RSV/5' LTR hybrid promoter 1 410
promoter CMV CMV promoter driving gene of interest 1809 2392
promoter PGK PGK promoter to drive selection marker in mammalian cells 4266 4771
recombination site attR2 attR2 recombinational cloning site 4127 4142
recombination site attR1 attR1 recombinational cloning site 2440 2456
response element HIV-1 RRE HIV-1 Rev response element (RRE) 1075 1308
selectable marker AmpR Ampicillin resistance gene for selection in bacteria 6728 7588
selectable marker BlastR Blasticidin resistance gene in mammalian cells 4849 5249
selectable marker CmR chloramphenicol resistance marker (removed in plasmids with insert) 2673 3329
splice acceptor site 3' splice acceptor two 3' splice acceptors at 1656 and 1684 1656 1656
splice donor site 5' splice donor 5' splice donor 520 520
tag V5 C-terminal V5 epitope tag 4197 4238

 

  • Gene
  • Pathways
  • Protein
  • Clone
  • Organism
  • Genome
  • Reagents

Gene

 

Gene Symbol : PAX4
Symbol Nomenclature : PAX4
SYNONYM : KPD; MODY9
Designation : paired box gene 4|paired box protein Pax-4|paired domain gene 4
Full Nomenclature : paired box 4
GENEID : 5078
HGNC : 8618
MIM : 167413
Vega : OTTHUMG00000157562
Target GenBank: BC074761

 

Reference Sequence Alignment

  

HsCD00329734        ------------------------ATGAACCAGCTTGGGGGGCTCTTTGTGAATGGCCGG
XM_005250397.1      ATGCATCAGGACGGGATCAGCAGCATGAACCAGCTTGGGGGGCTCTTTGTGAATGGCCGG
NM_006193.2         ------------------------ATGAACCAGCTTGGGGGGCTCTTTGTGAATGGCCGG
                                            ************************************

HsCD00329734        CCCCTGCCTCTGGATACCCGGCAGCAGATTGTGCGGCTAGCAGTCAGTGGAATGCGGCCC
XM_005250397.1      CCCCTGCCTCTGGATACCCGGCAGCAGATTGTGCGGCTAGCAGTCAGTGGAATGCGGCCC
NM_006193.2         CCCCTGCCTCTGGATACCCGGCAGCAGATTGTGCGGCTAGCAGTCAGTGGAATGCGGCCC
                    ************************************************************

HsCD00329734        TGTGACATCTCACGGATCCTTAAGGTATCTAATGGCTGTGTGAGCAAGATCCTAGGGCGT
XM_005250397.1      TGTGACATCTCACGGATCCTTAAGGTATCTAATGGCTGTGTGAGCAAGATCCTAGGGCGT
NM_006193.2         TGTGACATCTCACGGATCCTTAAGGTATCTAATGGCTGTGTGAGCAAGATCCTAGGGCGT
                    ************************************************************

HsCD00329734        TACTACCGCACAGGTGTCTTGGAGCCAAAGGGCATTGGGGGAAGCAAGCCACGGCTGGCT
XM_005250397.1      TACTACCGCACAGGTGTCTTGGAGCCAAAGGGCATTGGGGGAAGCAAGCCACGGCTGGCT
NM_006193.2         TACTACCGCACAGGTGTCTTGGAGCCAAAGGGCATTGGGGGAAGCAAGCCACGGCTGGCT
                    ************************************************************

HsCD00329734        ACACCCCCTGTGGTGGCTCGAATTGCCCAGCTGAAGGGTGAGTGTCCAGCCCTCTTTGCC
XM_005250397.1      ACACCCCCTGTGGTGGCTCGAATTGCCCAGCTGAAGGGTGAGTGTCCAGCCCTCTTTGCC
NM_006193.2         ACACCCCCTGTGGTGGCTCGAATTGCCCAGCTGAAGGGTGAGTGTCCAGCCCTCTTTGCC
                    ************************************************************

HsCD00329734        TGGGAAATCCAACGCCAGCTTTGTGCTGAAGGGCTTTGCACCCAGGACAAGACTCCCAGT
XM_005250397.1      TGGGAAATCCAACGCCAGCTTTGTGCTGAAGGGCTTTGCACCCAGGACAAGACTCCCAGT
NM_006193.2         TGGGAAATCCAACGCCAGCTTTGTGCTGAAGGGCTTTGCACCCAGGACAAGACTCCCAGT
                    ************************************************************

HsCD00329734        GTCTCCTCCATCAACCGAGTCCTGCGGGCATTACAGGAGGACCAGGGACTACCGTGCACA
XM_005250397.1      GTCTCCTCCATCAACCGAGTCCTGCGGGCATTACAGGAGGACCAGGGACTACCGTGCACA
NM_006193.2         GTCTCCTCCATCAACCGAGTCCTGCGGGCATTACAGGAGGACCAGGGACTACCGTGCACA
                    ************************************************************

HsCD00329734        CGGCTCAGGTCACCAGCTGTTTTGGCTCCAGCTGTCCTCACTCCCCATAGTGGCTCTGAG
XM_005250397.1      CGGCTCAGGTCACCAGCTGTTTTGGCTCCAGCTGTCCTCACTCCCCATAGTGGCTCTGAG
NM_006193.2         CGGCTCAGGTCACCAGCTGTTTTGGCTCCAGCTGTCCTCACTCCCCATAGTGGCTCTGAG
                    ************************************************************

HsCD00329734        ACTCCCCGGGGTACCCACCCAGGGACCGGCCACCGGAATCGGACTATCTTCTCCCCAAGC
XM_005250397.1      ACTCCCCGGGGTACCCACCCAGGGACCGGCCACCGGAATCGGACTATCTTCTCCCCAAGC
NM_006193.2         ACTCCCCGGGGTACCCACCCAGGGACCGGCCACCGGAATCGGACTATCTTCTCCCCAAGC
                    ************************************************************

HsCD00329734        CAGGCAGAGGCACTGGAGAAAGAGTTCCAGCGTGGGCAGTATCCTGATTCAGTGGCCCGT
XM_005250397.1      CAAGCAGAGGCACTGGAGAAAGAGTTCCAGCGTGGGCAGTATCCTGATTCAGTGGCCCGT
NM_006193.2         CAAGCAGAGGCACTGGAGAAAGAGTTCCAGCGTGGGCAGTATCCTGATTCAGTGGCCCGT
                    **.*********************************************************

HsCD00329734        GGAAAGCTGGCTACTGCCACCTCTCTGCCTGAGGACACGGTGAGGGTCTGGTTTTCCAAC
XM_005250397.1      GGAAAGCTGGCTACTGCCACCTCTCTGCCTGAGGACACGGTGAGGGTCTGGTTTTCCAAC
NM_006193.2         GGAAAGCTGGCTACTGCCACCTCTCTGCCTGAGGACACGGTGAGGGTCTGGTTTTCCAAC
                    ************************************************************

HsCD00329734        AGAAGAGCCAAATGGCGTCGGCAAGAGAAGCTCAAGTGGGAAATGCAGCTGCCAGGTGCT
XM_005250397.1      AGAAGAGCCAAATGGCGTCGGCAAGAGAAGCTCAAGTGGGAAATGCAGCTGCCAGGTGCT
NM_006193.2         AGAAGAGCCAAATGGCGTCGGCAAGAGAAGCTCAAGTGGGAAATGCAGCTGCCAGGTGCT
                    ************************************************************

HsCD00329734        TCCCAGGGGCTGACTGTACCAAGGGTTGCCCCAGGAATCATCTCTGCACAGCAGTCCCCT
XM_005250397.1      TCCCAGGGGCTGACTGTACCAAGGGTTGCCCCAGGAATCATCTCTGCACAGCAGTCCCCT
NM_006193.2         TCCCAGGGGCTGACTGTACCAAGGGTTGCCCCAGGAATCATCTCTGCACAGCAGTCCCCT
                    ************************************************************

HsCD00329734        GGCAGTGTGCCCACAGCAGCCCTGCCTGCCCTGGAACCACTGGGTCCCTCCTGCTATCAG
XM_005250397.1      GGCAGTGTGCCCACAGCAGCCCTGCCTGCCCTGGAACCACTGGGTCCCTCCTGCTATCAG
NM_006193.2         GGCAGTGTGCCCACAGCAGCCCTGCCTGCCCTGGAACCACTGGGTCCCTCCTGCTATCAG
                    ************************************************************

HsCD00329734        CTGTGCTGGGCAACAGCACCAGAAAGGTGTCTGAGTGACACCCCACCTAAAGCCTGTCTC
XM_005250397.1      CTGTGCTGGGCAACAGCACCAGAAAGGTGTCTGAGTGACACCCCACCTAAAGCCTGTCTC
NM_006193.2         CTGTGCTGGGCAACAGCACCAGAAAGGTGTCTGAGTGACACCCCACCTAAAGCCTGTCTC
                    ************************************************************

HsCD00329734        AAGCCCTGCTGGGGCCACTTGCCCCCACAGCCGAATTCCCTGGACTCAGGACTGCTTTGC
XM_005250397.1      AAGCCCTGCTGGGACTGTGGCTCCTTCCTCCTTCCTGTGATTGCTCCCTCCTGTGTGGAC
NM_006193.2         AAGCCCTGCTGGGGCCACTTGCCCCCACAGCCGAATTCCCTGGACTCAGGACTGCTTTGC
                    *************.* .     **  .*: *  ..*   .* *.  *.  .    *  .*

HsCD00329734        CTTCCTTGCCCTTCCTCCCACTGTCCCCTGGCCAGTCTTAGTGGCTCTCAGGCCCTGCTC
XM_005250397.1      GTTGCCTGGCCCTGCCTCGATGCCTCTCTGGCGCATCACCTGATTGGAGGGGCTGGTAAA
NM_006193.2         CTTCCTTGCCCTTCCTCCCACTGTCACCTGGCCAGTCTTAGTGGCTCTCAGGCCCTGCTC
                     ** * ** ** * *  * *     . ***** ..**: .  .    : .***    .:.

HsCD00329734        TGGCCTGGCTGCCCACTACTGTATGGCTTGGAATTG
XM_005250397.1      GCAACACCCACCCACTTCTCACACTAG---------
NM_006193.2         TGGCCTGGCTGCCCACTACTGTATGGCTTGGAATGA
                      ..*:  *: **.. *.  . *  .          

Pathways

Reactome : Developmental Biology
Reactome : Regulation of beta-cell development
Reactome : Regulation of gene expression in endocrine-committed (NEUROG3+) progenitor cells

Protein

SMART domain : PAX : Paired Box domain
SMART domain : HOX : Homeodomain
UniProt : O43316
HPRD : 01333

Clone

Cloning Information : 268471
HIP Master Clone ID : 193507
Original Clone ID : FLH268471.01L

Organism


TAX_ID : 9606
Species Specific ID: 5078

Genome

Chromosome : 7
Map Location : 7q32
Ensembl : ENSG00000106331

Reagents

Labome : PAX4-antibody