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TFDP3 (Homo sapiens) in pLenti6.2/V5-DEST (V5-tagged lentiviral mammalian expression vector)

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Explanation of Terms


Gene: Gene Symbol:  TFDP3
Gene Name:  transcription factor Dp family, member 3
Sequence             
Original Clone ID: FLH268473.01L
Keyword: None
Species: Homo sapiens
Type: cDNA
Vector Name: pLenti6.2/V5-DEST               Format:  FUSION
Source: HIP
Description: None
Comments: None
Mutation/
Discrepancy :
No / No
Publications: None
Authors:
HsCD00329736

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AVAILABLE
No restriction
Special MTA: None

 

Insert sequence: 1038nts         Open reading frame : 1 to 1038
Reference Sequence Alignment

ATGCCTCAGAGACCAGCAGCATCAAACATCCCTGTGGTAGGAAGCCCAAACCCACCCAGC
ACTCACTTTGCCTCTCAGAACCAGCATTCCTACTCCTCACCTCCTTGGGCCGGGCAGCAC
AACAGGAAAGGAGAGAAGAATGGCATGGGCCTGTGCCGTCTTTCCATGAAGGTCTGGGAG
ACGGTGCAGAGGAAAGGGACCACTTCCTGCCAGGAAGTGGTGGGCGAGCTGGTCGCCAAG
TTCAGAGCTGCCAGCAACCACGCCTCACCAAACGAGTCAGCTTATGACGTGAAAAACATA
AAACGGCGCACCTACGATGCCTTAAACGTGCTGATGGCCATGAATATCATCTCCAGGGAG
AAAAAGAAGATCAAGTGGATTGGTCTGACCACCAACTCGGCTCAGAACTGTCAGAACTTA
CGGGTGGAAAGACAGAAGAGACTTGAAAGAATAAAGCAGAAACAGTCTGAACTTCAACAA
CTTATTCTACAGCAAATTGCTTTCAAGAACCTGGTGCTGAGAAACCAGTATGTGGAGGAG
CAGGTCAGCCAGCGGCCGCTGCCCAACTCAGTCATCCACGTGCCCTTCATCATCATCAGC
AGTAGCAAGAAGACCGTCATCAACTGCAGCATCTCCGACGACAAATCAGAATATCTGTTT
AAGTTTAACAGCTCCTTTGAAATCCACGATGACACAGAAGTGCTGATGTGGATGGGCATG
ACTTTTGGGCTAGAGTCCGGGAGCTGCTCTGCCGAAGACCTTAAAATGGCCAGAAATTTG
GTCCCAAAGGCTCTGGAGCCGTACGTGACAGAAATGGCTCAGGGAACTTTTGGAGGTGTG
TTCACGACGGCAGGTTCCAGGTCTAATGGCACGTGGCTTTCTGCCAGTGACCTGACCAAC
ATTGCGATTGGGATGCTGGCCACAAGCTCCGGTGGATCTCAGTACAGTGGCTCCAGGGTG
GAGACCCCAGCAGTCGAGGAGGAAGAGGAGGAGGACAACAACGATGACGACCTCAGTGAG
AATGACGAGGATGACTTG


Coding Sequence Details
Insert Sequence Verified?: N Verification Method: Insert was fully sequenced in parent vector
Reference Sequence Annotations:
End on reference sequence 1066
Start on reference sequence 29
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
5p GTACAAAAAAGCAGGCTCCACC(atg-goi)

3' Linker Sequence:
5p (goi-stop)GACCCAGCTTTCTTGTAC

 

 Recommended Growth Condition:

Distributed in bacterial strain : DH5-alpha T1 phage resistant
Antibiotic Selection: Host Type:  bacterial    Marker:  ampicillin
Host Type:  mammalian    Marker:  blasticidin
Bacterial Selection Condition:  100 ug/mL ampicillin
Growth Condition:  Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:  Commonly used conditions for ampicillin resistant plasmid clones.
Protein Expression Results: None
Recommended expression in: Not Applicable
       DyNA Vector Map

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Vector Name: pLenti6.2/V5-DEST

pLenti6.2V5-DEST in advanced viewed

Synonyms: pLenti6.2/V5-DEST
Sequencing Primer: Forward:  CMV forward
Reverse:  V5 reverse
Description: Lentiviral mammalian expression vector;CMV promoter and C-terminal V5 tag; ampicillin resistance in bacteria; blasticidin resistance in mammalian cells; Gateway recombinational cloning.
Comments: None
Size (bp): 8813
Parent Vector: None
Empty Vector: None
Properties: Gateway, acceptor (destination), lentiviral infection, mammalian expression, recombinational cloning, with tag/fusion/marker
Author Name: Invitrogen
Publications: None
Vector Map:         Vector Sequence:
Sequence:

 



Vector Features:

Type Name Description Start Position End Position
bacterial origin pUC ori pUC origin of replication 7733 8406
death cassette ccdB ccdB death cassette (removed when insert is present) 3767 3976
packaging signal HIV-1 psi HIV-1 psi packaging signal 521 565
poly-A signal SV40 SV40 polyadenylation signal 5639 5770
promoter RSV RSV/5' LTR hybrid promoter 1 410
promoter CMV CMV promoter driving gene of interest 1809 2392
promoter PGK PGK promoter to drive selection marker in mammalian cells 4266 4771
recombination site attR2 attR2 recombinational cloning site 4127 4142
recombination site attR1 attR1 recombinational cloning site 2440 2456
response element HIV-1 RRE HIV-1 Rev response element (RRE) 1075 1308
selectable marker AmpR Ampicillin resistance gene for selection in bacteria 6728 7588
selectable marker BlastR Blasticidin resistance gene in mammalian cells 4849 5249
selectable marker CmR chloramphenicol resistance marker (removed in plasmids with insert) 2673 3329
splice acceptor site 3' splice acceptor two 3' splice acceptors at 1656 and 1684 1656 1656
splice donor site 5' splice donor 5' splice donor 520 520
tag V5 C-terminal V5 epitope tag 4197 4238

 

  • Gene
  • Protein
  • Clone
  • Organism
  • Genome
  • Reagents

Gene

 

Gene Symbol : TFDP3
Symbol Nomenclature : TFDP3
SYNONYM : CT30; DP4; HCA661
Designation : E2F-like protein|cancer/testis antigen 30|hepatocellular carcinoma-associated antigen 661|transcription factor Dp family member 3
Full Nomenclature : transcription factor Dp family, member 3
GENEID : 51270
Locus Tag : RP3-358H7.2
HGNC : 24603
MIM : 300772
Vega : OTTHUMG00000022433
Target GenBank: BC074762

 

Reference Sequence Alignment

  

HsCD00329736      ------------------------------------------------------------
NM_016521.2       ATGGCAAAATATGTCAGTCTCACTGAAGCTAACGAAGAACTCAAGGTCTTAATGGACGAG
                                                                              

HsCD00329736      ------------------------------------------------------------
NM_016521.2       AACCAGACCAGCCGCCCCGTGGCCGTTCACACCTCCACCGTGAACCCGCTCGGGAAGCAG
                                                                              

HsCD00329736      ------------------------------------------------------------
NM_016521.2       CTCTTGCCGAAAACCTTTGGACAGTCCAGTGTCAACATTGACCAGCAAGTGGTAATTGGT
                                                                              

HsCD00329736      ATGCCTCAGAGACCAGCAGCATCAAACATCCCTGTGGTAGGAAGCCCAAACCCACCCAGC
NM_016521.2       ATGCCTCAGAGACCAGCAGCATCAAACATCCCTGTGGTAGGAAGCCCAAACCCACCCAGC
                  ************************************************************

HsCD00329736      ACTCACTTTGCCTCTCAGAACCAGCATTCCTACTCCTCACCTCCTTGGGCCGGGCAGCAC
NM_016521.2       ACTCACTTTGCCTCTCAGAACCAGCATTCCTACTCCTCACCTCCTTGGGCCGGGCAGCAC
                  ************************************************************

HsCD00329736      AACAGGAAAGGAGAGAAGAATGGCATGGGCCTGTGCCGTCTTTCCATGAAGGTCTGGGAG
NM_016521.2       AACAGGAAAGGAGAGAAGAATGGCATGGGCCTGTGCCGTCTTTCCATGAAGGTCTGGGAG
                  ************************************************************

HsCD00329736      ACGGTGCAGAGGAAAGGGACCACTTCCTGCCAGGAAGTGGTGGGCGAGCTGGTCGCCAAG
NM_016521.2       ACGGTGCAGAGGAAAGGGACCACTTCCTGCCAGGAAGTGGTGGGCGAGCTGGTCGCCAAG
                  ************************************************************

HsCD00329736      TTCAGAGCTGCCAGCAACCACGCCTCACCAAACGAGTCAGCTTATGACGTGAAAAACATA
NM_016521.2       TTCAGAGCTGCCAGCAACCACGCCTCACCAAACGAGTCAGCTTATGACGTGAAAAACATA
                  ************************************************************

HsCD00329736      AAACGGCGCACCTACGATGCCTTAAACGTGCTGATGGCCATGAATATCATCTCCAGGGAG
NM_016521.2       AAACGGCGCACCTACGATGCCTTAAACGTGCTGATGGCCATGAATATCATCTCCAGGGAG
                  ************************************************************

HsCD00329736      AAAAAGAAGATCAAGTGGATTGGTCTGACCACCAACTCGGCTCAGAACTGTCAGAACTTA
NM_016521.2       AAAAAGAAGATCAAGTGGATTGGTCTGACCACCAACTCGGCTCAGAACTGTCAGAACTTA
                  ************************************************************

HsCD00329736      CGGGTGGAAAGACAGAAGAGACTTGAAAGAATAAAGCAGAAACAGTCTGAACTTCAACAA
NM_016521.2       CGGGTGGAAAGACAGAAGAGACTTGAAAGAATAAAGCAGAAACAGTCTGAACTTCAACAA
                  ************************************************************

HsCD00329736      CTTATTCTACAGCAAATTGCTTTCAAGAACCTGGTGCTGAGAAACCAGTATGTGGAGGAG
NM_016521.2       CTTATTCTACAGCAAATTGCTTTCAAGAACCTGGTGCTGAGAAACCAGTATGTGGAGGAG
                  ************************************************************

HsCD00329736      CAGGTCAGCCAGCGGCCGCTGCCCAACTCAGTCATCCACGTGCCCTTCATCATCATCAGC
NM_016521.2       CAGGTCAGCCAGCGGCCGCTGCCCAACTCAGTCATCCACGTGCCCTTCATCATCATCAGC
                  ************************************************************

HsCD00329736      AGTAGCAAGAAGACCGTCATCAACTGCAGCATCTCCGACGACAAATCAGAATATCTGTTT
NM_016521.2       AGTAGCAAGAAGACCGTCATCAACTGCAGCATCTCCGACGACAAATCAGAATATCTGTTT
                  ************************************************************

HsCD00329736      AAGTTTAACAGCTCCTTTGAAATCCACGATGACACAGAAGTGCTGATGTGGATGGGCATG
NM_016521.2       AAGTTTAACAGCTCCTTTGAAATCCACGATGACACAGAAGTGCTGATGTGGATGGGCATG
                  ************************************************************

HsCD00329736      ACTTTTGGGCTAGAGTCCGGGAGCTGCTCTGCCGAAGACCTTAAAATGGCCAGAAATTTG
NM_016521.2       ACTTTTGGGCTAGAGTCCGGGAGCTGCTCTGCCGAAGACCTTAAAATGGCCAGAAATTTG
                  ************************************************************

HsCD00329736      GTCCCAAAGGCTCTGGAGCCGTACGTGACAGAAATGGCTCAGGGAACTTTTGGAGGTGTG
NM_016521.2       GTCCCAAAGGCTCTGGAGCCGTACGTGACAGAAATGGCTCAGGGAACTTTTGGAGGTGTG
                  ************************************************************

HsCD00329736      TTCACGACGGCAGGTTCCAGGTCTAATGGCACGTGGCTTTCTGCCAGTGACCTGACCAAC
NM_016521.2       TTCACGACGGCAGGTTCCAGGTCTAATGGCACGTGGCTTTCTGCCAGTGACCTGACCAAC
                  ************************************************************

HsCD00329736      ATTGCGATTGGGATGCTGGCCACAAGCTCCGGTGGATCTCAGTACAGTGGCTCCAGGGTG
NM_016521.2       ATTGCGATTGGGATGCTGGCCACAAGCTCCGGTGGATCTCAGTACAGTGGCTCCAGGGTG
                  ************************************************************

HsCD00329736      GAGACCCCAGCAGTCGAGGAGGAAGAGGAGGAGGACAACAACGATGACGACCTCAGTGAG
NM_016521.2       GAGACCCCAGCAGTCGAGGAGGAAGAGGAGGAGGACAACAACGATGACGACCTCAGTGAG
                  ************************************************************

HsCD00329736      AATGACGAGGATGACTTG
NM_016521.2       AATGACGAGGATGACTGA
                  **************** .

Protein

SMART domain : DP : Transcription factor DP
UniProt : Q5H9I0
HPRD : 06623

Clone

Cloning Information : 268473
HIP Master Clone ID : 193511
Original Clone ID : FLH268473.01L

Organism


TAX_ID : 9606
Species Specific ID: 51270

Genome

Chromosome : X
Map Location : Xq26.2
Ensembl : ENSG00000183434

Reagents

Labome : TFDP3-antibody