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ELK4 (Homo sapiens) in pLenti6.2/V5-DEST (V5-tagged lentiviral mammalian expression vector)

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Explanation of Terms


Gene: Gene Symbol:  ELK4
Gene Name:  ELK4, ETS-domain protein (SRF accessory protein 1)
Sequence             
Original Clone ID: FLH268477.01L
Keyword: None
Species: Homo sapiens
Type: cDNA
Vector Name: pLenti6.2/V5-DEST               Format:  FUSION
Source: HIP
Description: None
Comments: None
Mutation/
Discrepancy :
No / No
Publications: None
Authors:
HsCD00329739

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AVAILABLE
No restriction
Special MTA: None

 

Insert sequence: 1218nts         Open reading frame : 1 to 1218
Reference Sequence Alignment

ATGGACAGTGCTATCACCCTGTGGCAGTTCCTTCTTCAGCTCCTGCAGAAGCCTCAGAAC
AAGCACATGATCTGTTGGACCTCTAATGATGGGCAGTTTAAGCTTTTGCAGGCAGAAGAG
GTGGCTCGTCTCTGGGGGATTCGCAAGAACAAGCCTAACATGAATTATGACAAACTCAGC
CGAGCCCTCAGATACTATTATGTAAAGAATATCATCAAAAAAGTGAATGGTCAGAAGTTT
GTGTACAAGTTTGTCTCTTATCCAGAGATTTTGAACATGGATCCAATGACAGTGGGCAGG
ATTGAGGGTGACTGTGAAAGTTTAAACTTCAGTGAAGTCAGCAGCAGTTCCAAAGATGTG
GAGAATGGAGGGAAAGATAAACCACCTCAGCCTGGTGCCAAGACCTCTAGCCGCAATGAC
TACATACACTCTGGCTTATATTCTTCATTTACTCTCAACTCTTTGAACTCCTCCAATGTA
AAGCTTTTCAAATTGATAAAGACTGAGAATCCAGCCGAGAAACTGGCAGAGAAAAAATCT
CCTCAGGAGCCCACACCATCTGTCATCAAATTTGTCACGACACCTTCCAAAAAGCCACCG
GTTGAACCTGTTGCTGCCACCATTTCAATTGGCCCAAGTATTTCTCCATCTTCAGAAGAA
ACTATCCAAGCTTTGGAGACATTGGTTTCCCCAAAACTGCCTTCCCTGGAAGCCCCAACC
TCTGCCTCTAACGTAATGACTGCTTTTGCCACCACACCACCCATTTCGTCCATACCCCCT
TTGCAGGAACCTCCCAGAACACCTTCACCACCACTGAGTTCTCACCCAGACATCGACACA
GACATTGATTCAGTGGCTTCTCAGCCAATGGAACTTCCAGAGAATTTGTCACTGGAGCCT
AAAGACCAGGATTCAGTCTTGCTAGAAAAGGACAAAGTAAATAATTCATCAAGATCCAAG
AAACCCAAAGGGTTAGAACTGGCACCCACCCTTGTGATCACGAGCAGTGATCCAAGCCCA
CTGGGAATACTGAGCCCATCTCTCCCTACAGCTTCTCTTACACCAGCATTTTTTTCACAG
GTAGCTTGCTCGCTCTTTATGGTGTCACCATTGCTTTCATTTATTTGCCCTTTTAAGCAA
ATCCAGAATTTATACACTCAAGTTTGCTTTCTGTTACTTAGGTTTGTCTTAGAAAGGTTA
TGTGTGACTGTCATGTTG


Coding Sequence Details
Insert Sequence Verified?: N Verification Method: Insert was fully sequenced in parent vector
Reference Sequence Annotations:
End on reference sequence 1248
Start on reference sequence 31
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
5p GTACAAAAAAGCAGGCTCCACC(atg-goi)

3' Linker Sequence:
5p (goi-stop)GACCCAGCTTTCTTGTAC

 

 Recommended Growth Condition:

Distributed in bacterial strain : DH5-alpha T1 phage resistant
Antibiotic Selection: Host Type:  bacterial    Marker:  ampicillin
Host Type:  mammalian    Marker:  blasticidin
Bacterial Selection Condition:  100 ug/mL ampicillin
Growth Condition:  Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:  Commonly used conditions for ampicillin resistant plasmid clones.
Protein Expression Results: None
Recommended expression in: Not Applicable
       DyNA Vector Map

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Vector Name: pLenti6.2/V5-DEST

pLenti6.2V5-DEST in advanced viewed

Synonyms: pLenti6.2/V5-DEST
Sequencing Primer: Forward:  CMV forward
Reverse:  V5 reverse
Description: Lentiviral mammalian expression vector;CMV promoter and C-terminal V5 tag; ampicillin resistance in bacteria; blasticidin resistance in mammalian cells; Gateway recombinational cloning.
Comments: None
Size (bp): 8813
Parent Vector: None
Empty Vector: None
Properties: Gateway, acceptor (destination), lentiviral infection, mammalian expression, recombinational cloning, with tag/fusion/marker
Author Name: Invitrogen
Publications: None
Vector Map:         Vector Sequence:
Sequence:

 



Vector Features:

Type Name Description Start Position End Position
bacterial origin pUC ori pUC origin of replication 7733 8406
death cassette ccdB ccdB death cassette (removed when insert is present) 3767 3976
packaging signal HIV-1 psi HIV-1 psi packaging signal 521 565
poly-A signal SV40 SV40 polyadenylation signal 5639 5770
promoter RSV RSV/5' LTR hybrid promoter 1 410
promoter CMV CMV promoter driving gene of interest 1809 2392
promoter PGK PGK promoter to drive selection marker in mammalian cells 4266 4771
recombination site attR2 attR2 recombinational cloning site 4127 4142
recombination site attR1 attR1 recombinational cloning site 2440 2456
response element HIV-1 RRE HIV-1 Rev response element (RRE) 1075 1308
selectable marker AmpR Ampicillin resistance gene for selection in bacteria 6728 7588
selectable marker BlastR Blasticidin resistance gene in mammalian cells 4849 5249
selectable marker CmR chloramphenicol resistance marker (removed in plasmids with insert) 2673 3329
splice acceptor site 3' splice acceptor two 3' splice acceptors at 1656 and 1684 1656 1656
splice donor site 5' splice donor 5' splice donor 520 520
tag V5 C-terminal V5 epitope tag 4197 4238

 

  • Gene
  • Pathways
  • Protein
  • Clone
  • Organism
  • Genome
  • Reagents

Gene

 

Gene Symbol : ELK4
Symbol Nomenclature : ELK4
SYNONYM : SAP1
Designation : ETS domain-containing protein Elk-4|SAP-1|serum response factor accessory protein 1
Full Nomenclature : ELK4, ETS-domain protein (SRF accessory protein 1)
GENEID : 2005
HGNC : 3326
MIM : 600246
Vega : OTTHUMG00000037221
Target GenBank: BC063676

 

Reference Sequence Alignment

  

HsCD00329739      ATGGACAGTGCTATCACCCTGTGGCAGTTCCTTCTTCAGCTCCTGCAGAAGCCTCAGAAC
NM_021795.2       ATGGACAGTGCTATCACCCTGTGGCAGTTCCTTCTTCAGCTCCTGCAGAAGCCTCAGAAC
NM_001973.3       ATGGACAGTGCTATCACCCTGTGGCAGTTCCTTCTTCAGCTCCTGCAGAAGCCTCAGAAC
                  ************************************************************

HsCD00329739      AAGCACATGATCTGTTGGACCTCTAATGATGGGCAGTTTAAGCTTTTGCAGGCAGAAGAG
NM_021795.2       AAGCACATGATCTGTTGGACCTCTAATGATGGGCAGTTTAAGCTTTTGCAGGCAGAAGAG
NM_001973.3       AAGCACATGATCTGTTGGACCTCTAATGATGGGCAGTTTAAGCTTTTGCAGGCAGAAGAG
                  ************************************************************

HsCD00329739      GTGGCTCGTCTCTGGGGGATTCGCAAGAACAAGCCTAACATGAATTATGACAAACTCAGC
NM_021795.2       GTGGCTCGTCTCTGGGGGATTCGCAAGAACAAGCCTAACATGAATTATGACAAACTCAGC
NM_001973.3       GTGGCTCGTCTCTGGGGGATTCGCAAGAACAAGCCTAACATGAATTATGACAAACTCAGC
                  ************************************************************

HsCD00329739      CGAGCCCTCAGATACTATTATGTAAAGAATATCATCAAAAAAGTGAATGGTCAGAAGTTT
NM_021795.2       CGAGCCCTCAGATACTATTATGTAAAGAATATCATCAAAAAAGTGAATGGTCAGAAGTTT
NM_001973.3       CGAGCCCTCAGATACTATTATGTAAAGAATATCATCAAAAAAGTGAATGGTCAGAAGTTT
                  ************************************************************

HsCD00329739      GTGTACAAGTTTGTCTCTTATCCAGAGATTTTGAACATGGATCCAATGACAGTGGGCAGG
NM_021795.2       GTGTACAAGTTTGTCTCTTATCCAGAGATTTTGAACATGGATCCAATGACAGTGGGCAGG
NM_001973.3       GTGTACAAGTTTGTCTCTTATCCAGAGATTTTGAACATGGATCCAATGACAGTGGGCAGG
                  ************************************************************

HsCD00329739      ATTGAGGGTGACTGTGAAAGTTTAAACTTCAGTGAAGTCAGCAGCAGTTCCAAAGATGTG
NM_021795.2       ATTGAGGGTGACTGTGAAAGTTTAAACTTCAGTGAAGTCAGCAGCAGTTCCAAAGATGTG
NM_001973.3       ATTGAGGGTGACTGTGAAAGTTTAAACTTCAGTGAAGTCAGCAGCAGTTCCAAAGATGTG
                  ************************************************************

HsCD00329739      GAGAATGGAGGGAAAGATAAACCACCTCAGCCTGGTGCCAAGACCTCTAGCCGCAATGAC
NM_021795.2       GAGAATGGAGGGAAAGATAAACCACCTCAGCCTGGTGCCAAGACCTCTAGCCGCAATGAC
NM_001973.3       GAGAATGGAGGGAAAGATAAACCACCTCAGCCTGGTGCCAAGACCTCTAGCCGCAATGAC
                  ************************************************************

HsCD00329739      TACATACACTCTGGCTTATATTCTTCATTTACTCTCAACTCTTTGAACTCCTCCAATGTA
NM_021795.2       TACATACACTCTGGCTTATATTCTTCATTTACTCTCAACTCTTTGAACTCCTCCAATGTA
NM_001973.3       TACATACACTCTGGCTTATATTCTTCATTTACTCTCAACTCTTTGAACTCCTCCAATGTA
                  ************************************************************

HsCD00329739      AAGCTTTTCAAATTGATAAAGACTGAGAATCCAGCCGAGAAACTGGCAGAGAAAAAATCT
NM_021795.2       AAGCTTTTCAAATTGATAAAGACTGAGAATCCAGCCGAGAAACTGGCAGAGAAAAAATCT
NM_001973.3       AAGCTTTTCAAATTGATAAAGACTGAGAATCCAGCCGAGAAACTGGCAGAGAAAAAATCT
                  ************************************************************

HsCD00329739      CCTCAGGAGCCCACACCATCTGTCATCAAATTTGTCACGACACCTTCCAAAAAGCCACCG
NM_021795.2       CCTCAGGAGCCCACACCATCTGTCATCAAATTTGTCACGACACCTTCCAAAAAGCCACCG
NM_001973.3       CCTCAGGAGCCCACACCATCTGTCATCAAATTTGTCACGACACCTTCCAAAAAGCCACCG
                  ************************************************************

HsCD00329739      GTTGAACCTGTTGCTGCCACCATTTCAATTGGCCCAAGTATTTCTCCATCTTCAGAAGAA
NM_021795.2       GTTGAACCTGTTGCTGCCACCATTTCAATTGGCCCAAGTATTTCTCCATCTTCAGAAGAA
NM_001973.3       GTTGAACCTGTTGCTGCCACCATTTCAATTGGCCCAAGTATTTCTCCATCTTCAGAAGAA
                  ************************************************************

HsCD00329739      ACTATCCAAGCTTTGGAGACATTGGTTTCCCCAAAACTGCCTTCCCTGGAAGCCCCAACC
NM_021795.2       ACTATCCAAGCTTTGGAGACATTGGTTTCCCCAAAACTGCCTTCCCTGGAAGCCCCAACC
NM_001973.3       ACTATCCAAGCTTTGGAGACATTGGTTTCCCCAAAACTGCCTTCCCTGGAAGCCCCAACC
                  ************************************************************

HsCD00329739      TCTGCCTCTAACGTAATGACTGCTTTTGCCACCACACCACCCATTTCGTCCATACCCCCT
NM_021795.2       TCTGCCTCTAACGTAATGACTGCTTTTGCCACCACACCACCCATTTCGTCCATACCCCCT
NM_001973.3       TCTGCCTCTAACGTAATGACTGCTTTTGCCACCACACCACCCATTTCGTCCATACCCCCT
                  ************************************************************

HsCD00329739      TTGCAGGAACCTCCCAGAACACCTTCACCACCACTGAGTTCTCACCCAGACATCGACACA
NM_021795.2       TTGCAGGAACCTCCCAGAACACCTTCACCACCACTGAGTTCTCACCCAGACATCGACACA
NM_001973.3       TTGCAGGAACCTCCCAGAACACCTTCACCACCACTGAGTTCTCACCCAGACATCGACACA
                  ************************************************************

HsCD00329739      GACATTGATTCAGTGGCTTCTCAGCCAATGGAACTTCCAGAGAATTTGTCACTGGAGCCT
NM_021795.2       GACATTGATTCAGTGGCTTCTCAGCCAATGGAACTTCCAGAGAATTTGTCACTGGAGCCT
NM_001973.3       GACATTGATTCAGTGGCTTCTCAGCCAATGGAACTTCCAGAGAATTTGTCACTGGAGCCT
                  ************************************************************

HsCD00329739      AAAGACCAGGATTCAGTCTTGCTAGAAAAGGACAAAGTAAATAATTCATCAAGATCCAAG
NM_021795.2       AAAGACCAGGATTCAGTCTTGCTAGAAAAGGACAAAGTAAATAATTCATCAAGATCCAAG
NM_001973.3       AAAGACCAGGATTCAGTCTTGCTAGAAAAGGACAAAGTAAATAATTCATCAAGATCCAAG
                  ************************************************************

HsCD00329739      AAACCCAAAGGGTTAGAACTGGCACCCACCCTTGTGATCACGAGCAGTGATCCAAGCCCA
NM_021795.2       AAACCCAAAGGGTTAGAACTGGCACCCACCCTTGTGATCACGAGCAGTGATCCAAGCCCA
NM_001973.3       AAACCCAAAGGGTTAGAACTGGCACCCACCCTTGTGATCACGAGCAGTGATCCAAGCCCA
                  ************************************************************

HsCD00329739      CTGGGAATACTGAGCCCATCTCTCCCTACAGCTTCTCTTACACCAGCATTTTTTTCACAG
NM_021795.2       CTGGGAATACTGAGCCCATCTCTCCCTACAGCTTCTCTTACACCAGCATTTTTTTCACAG
NM_001973.3       CTGGGAATACTGAGCCCATCTCTCCCTACAGCTTCTCTTACACCAGCATTTTTTTCACAG
                  ************************************************************

HsCD00329739      GTAGCTTGCTCGCTCTTTATGG-------TGTCACCATTGCTTTCATTTATTTGCCCTTT
NM_021795.2       GTAGCTTGCTCGCTCTTTATGG-------TGTCACCATTGCTTTCATTTATTTGCCCTTT
NM_001973.3       ACACCCATCATACTGACTCCAAGCCCCTTGCTCTCCAGTATCCACTTCTGGAGTACTCTC
                  . * * : *: .** : *. ..         **:*** *.   :*:* *. :  .*  * 

HsCD00329739      TAAGCAAATCCAGAATTTATACACTCAAGTTTGCTTTCTGTTACTTAGGTTTGTCTTAGA
NM_021795.2       TAAGCAAATCCAGAATTTATACACTCAAGTTTGCTTTCTGTTACTTAGGTTTGTCTTAGA
NM_001973.3       AGTCCTGTTGCTCCCCTAAGTCCAGCCAGACTGCAAGGTGCTAACACACTTTTCCAGTTT
                  :.: *:.:* *: .. *:* :*.. *.**: ***::  ** **. :.. ***  *: : :

HsCD00329739      AAGGTTATGTGTGACTGTCATGTTG-----------------------------------
NM_021795.2       AAGGTTATGTGTGACTGTCATGTGA-----------------------------------
NM_001973.3       CCTTCTGTACTGAACAGTCATGGGCCATTCACTCTGTCTGGGCTGGATGGACCTTCCACC
                  ..   *.*.   .**:******                                      

HsCD00329739      ------------------------------------
NM_021795.2       ------------------------------------
NM_001973.3       CCTGGCCCATTTTCCCCAGACCTACAGAAGACATAA
                                                      

Pathways

NCI : Signaling mediated by p38-alpha and p38-beta
Panther : Interleukin signaling pathway
Panther : PDGF signaling pathway
WikiPathway : EGF/EGFR Signaling Pathway
WikiPathway : ID signaling pathway
WikiPathway : Serotonin HTR1 Group and FOS Pathway
WikiPathway : Serotonin Receptor 2 and ELK-SRF/GATA4 signaling
WikiPathway : Serotonin Receptor 4/6/7 and NR3C Signaling

Protein

SMART domain : ETS : erythroblast transformation specific domain
UniProt : Q8IXL1
UniProt : P28324
HPRD : 02592

Clone

Cloning Information : 268477
HIP Master Clone ID : 193518
Original Clone ID : FLH268477.01L

Organism


TAX_ID : 9606
Species Specific ID: 2005

Genome

Chromosome : 1
Map Location : 1q32
Ensembl : ENSG00000158711

Reagents

Labome : ELK4-antibody