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ZSCAN4 (Homo sapiens) in pLenti6.2/V5-DEST (V5-tagged lentiviral mammalian expression vector)

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Explanation of Terms


Gene: Gene Symbol:  ZSCAN4
Gene Name:  zinc finger and SCAN domain containing 4
Sequence             
Original Clone ID: FLH268485.01L
Keyword: None
Species: Homo sapiens
Type: cDNA
Vector Name: pLenti6.2/V5-DEST               Format:  FUSION
Source: HIP
Description: None
Comments: None
Mutation/
Discrepancy :
No / No
Publications: None
Authors:
HsCD00329745

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AVAILABLE
No restriction
Special MTA: None

 

Insert sequence: 1302nts         Open reading frame : 1 to 1302
Reference Sequence Alignment

ATGGCTTTAGATCTAAGAACCATATTTCAGTGTGAACCATCCGAGAATAATCTTGGATCA
GAAAATTCAGCGTTTCAACAAAGCCAAGGACCTGCTGTTCAGAGAGAAGAAGGGATTTCT
GAGTTCTCAAGAATGGTGCTCAATTCATTTCAAGACAGCAATAATTCATATGCAAGGCAG
GAATTGCAAAGACTTTATAGGATCTTTCACTCATGGCTGCAACCAGAAAAGCACAGCAAG
GATGAAATTATTTCTCTATTAGTCCTGGAGCAGTTTATGATTGGTGGCCACTGCAATGAC
AAAGCCAGTGTGAAAGAGAAATGGAAATCAAGTGGCAAAAACTTGGAGAGATTCATAGAA
GACCTGACTGATGACAGCATAAATCCACCTGCCTTAGTCCACGTCCACATGCAGGGACAG
GAAGCTCTCTTTTCTGAGGATATGCCCTTAAGAGATGTCATTGTTCATCTCACAAAACAA
GTGAATGCCCAAACCACAAGAGAAGCAAACATGGGGACACCCTCCCAGACTTCCCAAGAT
ACTTCCTTAGAAACAGGACAAGGATATGAAGATGAACAAGATGGCTGGAACAGTTCTTCG
AAAACTACTCGAGTAAATGAAAATATTACTAATCAAGGCAATCAAATAGTTTCCCTAATC
ATCATCCAGGAAGAGAACGGTCCTAGGCCTGAAGAGGGAGGTGTTTCTTCTGACAACCCA
TACAACTCAAAAAGAGCAGAGCTAGTCACTGCTAGATCTCAGGAAGGGTCCATAAATGGA
ATCACTTTCCAAGGTGTCCCTATGGTGATGGGAGCAGGGTGTATCTCTCAACCAGAGCAG
TCCTCCCCTGAGTCTGCCCTTACCCACCAGAGCAATGAGGGAAATTCCACATGTGAGGTA
CATCAGAAAGGATCCCATGGAGTCCAAAAATCATACAAATGTGAAGAATGCCCCAAGGTC
TTTAAGTATCTCTGTCACTTATTAGCTCACCAGAGAAGACACAGGAATGAGAGGCCATTT
GTTTGTCCCGAGTGTCAAAAAGGCTTCTTCCAGATATCAGACCTACGGGTGCATCAGATA
ATTCACACAGGAAAGAAGCCTTTCACATGCAGCATGTGTAAAAAGTCCTTCAGCCACAAA
ACCAACCTGCGGTCTCATGAGAGAATCCACACAGGAGAAAAGCCTTATACATGTCCCTTT
TGTAAGACAAGCTACCGCCAGTCATCCACATACCACCGCCATATGAGGACTCATGAGAAA
ATTACCCTGCCAAGTGTTCCCTCCACACCAGAAGCTTCCTTG


Coding Sequence Details
Insert Sequence Verified?: N Verification Method: Insert was fully sequenced in parent vector
Reference Sequence Annotations:
End on reference sequence 1390
Start on reference sequence 89
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
5p GTACAAAAAAGCAGGCTCCACC(atg-goi)

3' Linker Sequence:
5p (goi-stop)GACCCAGCTTTCTTGTAC

 

 Recommended Growth Condition:

Distributed in bacterial strain : DH5-alpha T1 phage resistant
Antibiotic Selection: Host Type:  bacterial    Marker:  ampicillin
Host Type:  mammalian    Marker:  blasticidin
Bacterial Selection Condition:  100 ug/mL ampicillin
Growth Condition:  Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:  Commonly used conditions for ampicillin resistant plasmid clones.
Protein Expression Results: None
Recommended expression in: Not Applicable
       DyNA Vector Map

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Vector Name: pLenti6.2/V5-DEST

pLenti6.2V5-DEST in advanced viewed

Synonyms: pLenti6.2/V5-DEST
Sequencing Primer: Forward:  CMV forward
Reverse:  V5 reverse
Description: Lentiviral mammalian expression vector;CMV promoter and C-terminal V5 tag; ampicillin resistance in bacteria; blasticidin resistance in mammalian cells; Gateway recombinational cloning.
Comments: None
Size (bp): 8813
Parent Vector: None
Empty Vector: None
Properties: Gateway, acceptor (destination), lentiviral infection, mammalian expression, recombinational cloning, with tag/fusion/marker
Author Name: Invitrogen
Publications: None
Vector Map:         Vector Sequence:
Sequence:

 



Vector Features:

Type Name Description Start Position End Position
bacterial origin pUC ori pUC origin of replication 7733 8406
death cassette ccdB ccdB death cassette (removed when insert is present) 3767 3976
packaging signal HIV-1 psi HIV-1 psi packaging signal 521 565
poly-A signal SV40 SV40 polyadenylation signal 5639 5770
promoter RSV RSV/5' LTR hybrid promoter 1 410
promoter CMV CMV promoter driving gene of interest 1809 2392
promoter PGK PGK promoter to drive selection marker in mammalian cells 4266 4771
recombination site attR2 attR2 recombinational cloning site 4127 4142
recombination site attR1 attR1 recombinational cloning site 2440 2456
response element HIV-1 RRE HIV-1 Rev response element (RRE) 1075 1308
selectable marker AmpR Ampicillin resistance gene for selection in bacteria 6728 7588
selectable marker BlastR Blasticidin resistance gene in mammalian cells 4849 5249
selectable marker CmR chloramphenicol resistance marker (removed in plasmids with insert) 2673 3329
splice acceptor site 3' splice acceptor two 3' splice acceptors at 1656 and 1684 1656 1656
splice donor site 5' splice donor 5' splice donor 520 520
tag V5 C-terminal V5 epitope tag 4197 4238

 

  • Gene
  • Protein
  • Clone
  • Organism
  • Genome
  • Reagents

Gene

 

Gene Symbol : ZSCAN4
Symbol Nomenclature : ZSCAN4
SYNONYM : ZNF494
Designation : zinc finger and SCAN domain-containing protein 4|zinc finger protein 494
Full Nomenclature : zinc finger and SCAN domain containing 4
GENEID : 201516
HGNC : 23709
MIM : 613419
Vega : OTTHUMG00000183474
Target GenBank: BC101738

 

Reference Sequence Alignment

  

HsCD00329745      ATGGCTTTAGATCTAAGAACCATATTTCAGTGTGAACCATCCGAGAATAATCTTGGATCA
NM_152677.2       ATGGCTTTAGATCTAAGAACCATATTTCAGTGTGAACCATCCGAGAATAATCTTGGATCA
                  ************************************************************

HsCD00329745      GAAAATTCAGCGTTTCAACAAAGCCAAGGACCTGCTGTTCAGAGAGAAGAAGGGATTTCT
NM_152677.2       GAAAATTCAGCGTTTCAACAAAGCCAAGGACCTGCTGTTCAGAGAGAAGAAGGGATTTCT
                  ************************************************************

HsCD00329745      GAGTTCTCAAGAATGGTGCTCAATTCATTTCAAGACAGCAATAATTCATATGCAAGGCAG
NM_152677.2       GAGTTCTCAAGAATGGTGCTCAATTCATTTCAAGACAGCAATAATTCATATGCAAGGCAG
                  ************************************************************

HsCD00329745      GAATTGCAAAGACTTTATAGGATCTTTCACTCATGGCTGCAACCAGAAAAGCACAGCAAG
NM_152677.2       GAATTGCAAAGACTTTATAGGATCTTTCACTCATGGCTGCAACCAGAAAAGCACAGCAAG
                  ************************************************************

HsCD00329745      GATGAAATTATTTCTCTATTAGTCCTGGAGCAGTTTATGATTGGTGGCCACTGCAATGAC
NM_152677.2       GATGAAATTATTTCTCTATTAGTCCTGGAGCAGTTTATGATTGGTGGCCACTGCAATGAC
                  ************************************************************

HsCD00329745      AAAGCCAGTGTGAAAGAGAAATGGAAATCAAGTGGCAAAAACTTGGAGAGATTCATAGAA
NM_152677.2       AAAGCCAGTGTGAAAGAGAAATGGAAATCAAGTGGCAAAAACTTGGAGAGATTCATAGAA
                  ************************************************************

HsCD00329745      GACCTGACTGATGACAGCATAAATCCACCTGCCTTAGTCCACGTCCACATGCAGGGACAG
NM_152677.2       GACCTGACTGATGACAGCATAAATCCACCTGCCTTAGTCCACGTCCACATGCAGGGACAG
                  ************************************************************

HsCD00329745      GAAGCTCTCTTTTCTGAGGATATGCCCTTAAGAGATGTCATTGTTCATCTCACAAAACAA
NM_152677.2       GAAGCTCTCTTTTCTGAGGATATGCCCTTAAGAGATGTCATTGTTCATCTCACAAAACAA
                  ************************************************************

HsCD00329745      GTGAATGCCCAAACCACAAGAGAAGCAAACATGGGGACACCCTCCCAGACTTCCCAAGAT
NM_152677.2       GTGAATGCCCAAACCACAAGAGAAGCAAACATGGGGACACCCTCCCAGACTTCCCAAGAT
                  ************************************************************

HsCD00329745      ACTTCCTTAGAAACAGGACAAGGATATGAAGATGAACAAGATGGCTGGAACAGTTCTTCG
NM_152677.2       ACTTCCTTAGAAACAGGACAAGGATATGAAGATGAACAAGATGGCTGGAACAGTTCTTCG
                  ************************************************************

HsCD00329745      AAAACTACTCGAGTAAATGAAAATATTACTAATCAAGGCAATCAAATAGTTTCCCTAATC
NM_152677.2       AAAACTACTCGAGTAAATGAAAATATTACTAATCAAGGCAATCAAATAGTTTCCCTAATC
                  ************************************************************

HsCD00329745      ATCATCCAGGAAGAGAACGGTCCTAGGCCTGAAGAGGGAGGTGTTTCTTCTGACAACCCA
NM_152677.2       ATCATCCAGGAAGAGAACGGTCCTAGGCCTGAAGAGGGAGGTGTTTCTTCTGACAACCCA
                  ************************************************************

HsCD00329745      TACAACTCAAAAAGAGCAGAGCTAGTCACTGCTAGATCTCAGGAAGGGTCCATAAATGGA
NM_152677.2       TACAACTCAAAAAGAGCAGAGCTAGTCACTGCTAGATCTCAGGAAGGGTCCATAAATGGA
                  ************************************************************

HsCD00329745      ATCACTTTCCAAGGTGTCCCTATGGTGATGGGAGCAGGGTGTATCTCTCAACCAGAGCAG
NM_152677.2       ATCACTTTCCAAGGTGTCCCTATGGTGATGGGAGCAGGGTGTATCTCTCAACCAGAGCAG
                  ************************************************************

HsCD00329745      TCCTCCCCTGAGTCTGCCCTTACCCACCAGAGCAATGAGGGAAATTCCACATGTGAGGTA
NM_152677.2       TCCTCCCCTGAGTCTGCCCTTACCCACCAGAGCAATGAGGGAAATTCCACATGTGAGGTA
                  ************************************************************

HsCD00329745      CATCAGAAAGGATCCCATGGAGTCCAAAAATCATACAAATGTGAAGAATGCCCCAAGGTC
NM_152677.2       CATCAGAAAGGATCCCATGGAGTCCAAAAATCATACAAATGTGAAGAATGCCCCAAGGTC
                  ************************************************************

HsCD00329745      TTTAAGTATCTCTGTCACTTATTAGCTCACCAGAGAAGACACAGGAATGAGAGGCCATTT
NM_152677.2       TTTAAGTATCTCTGTCACTTATTAGCTCACCAGAGAAGACACAGGAATGAGAGGCCATTT
                  ************************************************************

HsCD00329745      GTTTGTCCCGAGTGTCAAAAAGGCTTCTTCCAGATATCAGACCTACGGGTGCATCAGATA
NM_152677.2       GTTTGTCCCGAGTGTCAAAAAGGCTTCTTCCAGATATCAGACCTACGGGTGCATCAGATA
                  ************************************************************

HsCD00329745      ATTCACACAGGAAAGAAGCCTTTCACATGCAGCATGTGTAAAAAGTCCTTCAGCCACAAA
NM_152677.2       ATTCACACAGGAAAGAAGCCTTTCACATGCAGCATGTGTAAAAAGTCCTTCAGCCACAAA
                  ************************************************************

HsCD00329745      ACCAACCTGCGGTCTCATGAGAGAATCCACACAGGAGAAAAGCCTTATACATGTCCCTTT
NM_152677.2       ACCAACCTGCGGTCTCATGAGAGAATCCACACAGGAGAAAAGCCTTATACATGTCCCTTT
                  ************************************************************

HsCD00329745      TGTAAGACAAGCTACCGCCAGTCATCCACATACCACCGCCATATGAGGACTCATGAGAAA
NM_152677.2       TGTAAGACAAGCTACCGCCAGTCATCCACATACCACCGCCATATGAGGACTCATGAGAAA
                  ************************************************************

HsCD00329745      ATTACCCTGCCAAGTGTTCCCTCCACACCAGAAGCTTCCTTG
NM_152677.2       ATTACCCTGCCAAGTGTTCCCTCCACACCAGAAGCTTCCTAA
                  ****************************************:.

Protein

SMART domain : ZnF_C2H2 : zinc finger
SMART domain : SCAN : leucine rich region
UniProt : Q8NAM6
HPRD : 15899

Clone

Cloning Information : 268485
HIP Master Clone ID : 193527
Original Clone ID : FLH268485.01L

Organism


TAX_ID : 9606
Species Specific ID: 201516

Genome

Chromosome : 19
Map Location : 19q13.43
Ensembl : ENSG00000180532

Reagents

Labome : ZSCAN4-antibody