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COPS2 (Homo sapiens) in pLenti6.2/V5-DEST (V5-tagged lentiviral mammalian expression vector)

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Explanation of Terms


Gene: Gene Symbol:  COPS2
Gene Name:  COP9 signalosome subunit 2
Sequence             
Original Clone ID: FLH268486.01L
Keyword: None
Species: Homo sapiens
Type: cDNA
Vector Name: pLenti6.2/V5-DEST               Format:  FUSION
Source: HIP
Description: None
Comments: None
Mutation/
Discrepancy :
No / Yes
Publications: None
Authors:
HsCD00329746

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AVAILABLE
No restriction
Special MTA: None

 

Insert sequence: 1332nts         Open reading frame : 1 to 1332
Reference Sequence Alignment

ATGTCTGACATGGAGGATGATTTCATGTGCGATGATGAGGAGGACTACGACCTGGAATAC
TCTGAAGATAGTAACTCCGAGCCAAATGTGGATTTGGAAAATCAGTACTATAATTCCAAA
GCATTAAAAGAAGATGACCCAAAAGCGGCATTAAGCAGTTTCCAAAAGGTTTTGGAACTT
GAAGGTGAAAAAGGAGAATGGGGATTTAAAGCACTGAAACAAATGATTAAGATTAACTTC
AAGTTGACAAACTTTCCAGAAATGATGAATAGATATAAGCAGCTATTGACCTATATTCGG
AGTGCAGTCACAAGAAATTATTCTGAAAAATCCATTAATTCTATTCTTGATTATATCTCT
ACTTCTAAACAGATGGATTTACTGCAGGAATTCTATGAAACAACACTGGAAGCTTTGAAA
GATGCTAAGAATGATAGACTGTGGTTTAAGACAAACACAAAGCTTGGAAAATTATATTTA
GAACGAGAGGAATATGGAAAGCTTCAAAAAATTTTACGCCAGTTACATCAGTCGTGCCAG
ACTGATGATGGAGAAGATGATCTGAAAAAAGGTACACAGTTATTAGAAATATATGCTTTG
GAAATTCAAATGTACACAGCACAGAAAAATAACAAAAAACTTAAAGCACTCTATGAACAG
TCACTTCACATCAAGTCTGCCATCCCTCATCCACTGATTATGGGAGTTATCAGAGAATGT
GGTGGTAAAATGCACTTGAGGGAAGGTGAATTTGAAAAGGCACACACTGATTTTTTTGAA
GCCTTCAAGAATTATGATGAATCTGGAAGTCCAAGACGAACCACTTGCTTAAAATATTTG
GTCTTAGCAAATATGCTTATGAAATCGGGAATAAATCCATTTGACTCACAGGAGGCCAAG
CCGTACAAAAATGATCCAGAAATTTTAGCAATGACGAATTTAGTAAGTGCCTATCAGAAT
AATGACATCACTGAATTTGAAAAGATTCTAAAAACAAATCACAGCAACATCATGGATGAT
CCTTTCATAAGAGAACACATTGAAGAGCTTTTGCGAAACACCAGAACACAAGTGCTTATA
AAATTAATTAAGCCTTACACAAGAATACATATTCCTTTTATTTCTAAGGAGTTAAACATA
GATGTAGCTGATGTGGAGAGCTTGCTGGTGCAGTGCATATTGGATAACACTATTCATGGC
CGAATTGATCAAGTCAACCAACTCCTTGAACTGGATCATCAGAAGAGGGGTGGTGCACGA
TATACTGCACTAGATAAATGGACCAACCAACTAAATTCTCTCAACCAGGCTGTAGTCAGT
AAACTGGCTTTG


Coding Sequence Details
Insert Sequence Verified?: N Verification Method: Insert was fully sequenced in parent vector
Reference Sequence Annotations:
End on reference sequence 1348
Start on reference sequence 17
Mutations:
None
Discrepancy:
t1061c,I354T
                                

5' Linker Sequence:
5p GTACAAAAAAGCAGGCTCCACC(atg-goi)

3' Linker Sequence:
5p (goi-stop)GACCCAGCTTTCTTGTAC

 

 Recommended Growth Condition:

Distributed in bacterial strain : DH5-alpha T1 phage resistant
Antibiotic Selection: Host Type:  bacterial    Marker:  ampicillin
Host Type:  mammalian    Marker:  blasticidin
Bacterial Selection Condition:  100 ug/mL ampicillin
Growth Condition:  Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:  Commonly used conditions for ampicillin resistant plasmid clones.
Protein Expression Results: None
Recommended expression in: Not Applicable
       DyNA Vector Map

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Vector Name: pLenti6.2/V5-DEST

pLenti6.2V5-DEST in advanced viewed

Synonyms: pLenti6.2/V5-DEST
Sequencing Primer: Forward:  CMV forward
Reverse:  V5 reverse
Description: Lentiviral mammalian expression vector;CMV promoter and C-terminal V5 tag; ampicillin resistance in bacteria; blasticidin resistance in mammalian cells; Gateway recombinational cloning.
Comments: None
Size (bp): 8813
Parent Vector: None
Empty Vector: None
Properties: Gateway, acceptor (destination), lentiviral infection, mammalian expression, recombinational cloning, with tag/fusion/marker
Author Name: Invitrogen
Publications: None
Vector Map:         Vector Sequence:
Sequence:

 



Vector Features:

Type Name Description Start Position End Position
bacterial origin pUC ori pUC origin of replication 7733 8406
death cassette ccdB ccdB death cassette (removed when insert is present) 3767 3976
packaging signal HIV-1 psi HIV-1 psi packaging signal 521 565
poly-A signal SV40 SV40 polyadenylation signal 5639 5770
promoter RSV RSV/5' LTR hybrid promoter 1 410
promoter CMV CMV promoter driving gene of interest 1809 2392
promoter PGK PGK promoter to drive selection marker in mammalian cells 4266 4771
recombination site attR2 attR2 recombinational cloning site 4127 4142
recombination site attR1 attR1 recombinational cloning site 2440 2456
response element HIV-1 RRE HIV-1 Rev response element (RRE) 1075 1308
selectable marker AmpR Ampicillin resistance gene for selection in bacteria 6728 7588
selectable marker BlastR Blasticidin resistance gene in mammalian cells 4849 5249
selectable marker CmR chloramphenicol resistance marker (removed in plasmids with insert) 2673 3329
splice acceptor site 3' splice acceptor two 3' splice acceptors at 1656 and 1684 1656 1656
splice donor site 5' splice donor 5' splice donor 520 520
tag V5 C-terminal V5 epitope tag 4197 4238

 

  • Gene
  • Protein
  • Clone
  • Organism
  • Genome
  • Reagents

Gene

 

Gene Symbol : COPS2
Symbol Nomenclature : COPS2
SYNONYM : ALIEN; CSN2; SGN2; TRIP15
Designation : COP9 constitutive photomorphogenic homolog subunit 2|COP9 signalosome complex subunit 2|JAB1-containing signalosome subunit 2|TR-interacting protein 15|TRIP-15|alien homolog|signalosome subunit 2|thyroid receptor interacting protein 15|thyroid receptor-interacting protein 15
Full Nomenclature : COP9 signalosome subunit 2
GENEID : 9318
HGNC : 30747
MIM : 604508
Vega : OTTHUMG00000172324
Target GenBank: BC012629

 

Reference Sequence Alignment

  

HsCD00329746        ATGTCTGACATGGAGGATGATTTCATGTGCGATGATGAGGAGGACTACGACCTGGAATAC
NM_004236.3         ATGTCTGACATGGAGGATGATTTCATGTGCGATGATGAGGAGGACTACGACCTGGAATAC
NM_001143887.1      ATGTCTGACATGGAGGATGATTTCATGTGCGATGATGAGGAGGACTACGACCTGGAATAC
                    ************************************************************

HsCD00329746        TCTGAAGATAGTAACTCCGAGCCAAATGTGGATTTGGAAAATCAGTACTATAATTCCAAA
NM_004236.3         TCTGAAGATAGTAACTCCGAGCCAAATGTGGATTTGGAAAATCAGTACTATAATTCCAAA
NM_001143887.1      TCTGAAGATAGTAACTCCGAGCCAAATGTGGATTTGGAAAATCAGTACTATAATTCCAAA
                    ************************************************************

HsCD00329746        GCATTAAAAGAAGATGACCCAAAAGCGGCATTAAGCAGTTTCCAAAAGGTTTTGGAACTT
NM_004236.3         GCATTAAAAGAAGATGACCCAAAAGCGGCATTAAGCAGTTTCCAAAAGGTTTTGGAACTT
NM_001143887.1      GCATTAAAAGAAGATGACCCAAAAGCGGCATTAAGCAGTTTCCAAAAGGTTTTGGAACTT
                    ************************************************************

HsCD00329746        GAAGGTGAAAAAGGAGAATGGGGATTTAAAGCACTGAAACAAATGATTAAGATTAACTTC
NM_004236.3         GAAGGTGAAAAAGGAGAATGGGGATTTAAAGCACTGAAACAAATGATTAAGATTAACTTC
NM_001143887.1      GAAGGTGAAAAAGGAGAATGGGGATTTAAAGCACTGAAACAAATGATTAAGATTAACTTC
                    ************************************************************

HsCD00329746        AAGTTGACAAACTTTCCAGAAATGATGAATAGATATAAGCAGCTATTGACCTATATTCGG
NM_004236.3         AAGTTGACAAACTTTCCAGAAATGATGAATAGATATAAGCAGCTATTGACCTATATTCGG
NM_001143887.1      AAGTTGACAAACTTTCCAGAAATGATGAATAGATATAAGCAGCTATTGACCTATATTCGG
                    ************************************************************

HsCD00329746        AGTGCAGTCACAAGAAATTATTCTGAAAAATCCATTAATTCTATTCTTGATTATATCTCT
NM_004236.3         AGTGCAGTCACAAGAAATTATTCTGAAAAATCCATTAATTCTATTCTTGATTATATCTCT
NM_001143887.1      AGTGCAGTCACAAGAAATTATTCTGAAAAATCCATTAATTCTATTCTTGATTATATCTCT
                    ************************************************************

HsCD00329746        ACTTCTAAACAGATG------------------------GATTTACTGCAGGAATTCTAT
NM_004236.3         ACTTCTAAACAG---ATG---------------------GATTTACTGCAGGAATTCTAT
NM_001143887.1      ACTTCTAAACAG---AATTCTGATTTTTTATGTCAGATGGATTTACTGCAGGAATTCTAT
                    ************                           *********************

HsCD00329746        GAAACAACACTGGAAGCTTTGAAAGATGCTAAGAATGATAGACTGTGGTTTAAGACAAAC
NM_004236.3         GAAACAACACTGGAAGCTTTGAAAGATGCTAAGAATGATAGACTGTGGTTTAAGACAAAC
NM_001143887.1      GAAACAACACTGGAAGCTTTGAAAGATGCTAAGAATGATAGACTGTGGTTTAAGACAAAC
                    ************************************************************

HsCD00329746        ACAAAGCTTGGAAAATTATATTTAGAACGAGAGGAATATGGAAAGCTTCAAAAAATTTTA
NM_004236.3         ACAAAGCTTGGAAAATTATATTTAGAACGAGAGGAATATGGAAAGCTTCAAAAAATTTTA
NM_001143887.1      ACAAAGCTTGGAAAATTATATTTAGAACGAGAGGAATATGGAAAGCTTCAAAAAATTTTA
                    ************************************************************

HsCD00329746        CGCCAGTTACATCAGTCGTGCCAGACTGATGATGGAGAAGATGATCTGAAAAAAGGTACA
NM_004236.3         CGCCAGTTACATCAGTCGTGCCAGACTGATGATGGAGAAGATGATCTGAAAAAAGGTACA
NM_001143887.1      CGCCAGTTACATCAGTCGTGCCAGACTGATGATGGAGAAGATGATCTGAAAAAAGGTACA
                    ************************************************************

HsCD00329746        CAGTTATTAGAAATATATGCTTTGGAAATTCAAATGTACACAGCACAGAAAAATAACAAA
NM_004236.3         CAGTTATTAGAAATATATGCTTTGGAAATTCAAATGTACACAGCACAGAAAAATAACAAA
NM_001143887.1      CAGTTATTAGAAATATATGCTTTGGAAATTCAAATGTACACAGCACAGAAAAATAACAAA
                    ************************************************************

HsCD00329746        AAACTTAAAGCACTCTATGAACAGTCACTTCACATCAAGTCTGCCATCCCTCATCCACTG
NM_004236.3         AAACTTAAAGCACTCTATGAACAGTCACTTCACATCAAGTCTGCCATCCCTCATCCACTG
NM_001143887.1      AAACTTAAAGCACTCTATGAACAGTCACTTCACATCAAGTCTGCCATCCCTCATCCACTG
                    ************************************************************

HsCD00329746        ATTATGGGAGTTATCAGAGAATGTGGTGGTAAAATGCACTTGAGGGAAGGTGAATTTGAA
NM_004236.3         ATTATGGGAGTTATCAGAGAATGTGGTGGTAAAATGCACTTGAGGGAAGGTGAATTTGAA
NM_001143887.1      ATTATGGGAGTTATCAGAGAATGTGGTGGTAAAATGCACTTGAGGGAAGGTGAATTTGAA
                    ************************************************************

HsCD00329746        AAGGCACACACTGATTTTTTTGAAGCCTTCAAGAATTATGATGAATCTGGAAGTCCAAGA
NM_004236.3         AAGGCACACACTGATTTTTTTGAAGCCTTCAAGAATTATGATGAATCTGGAAGTCCAAGA
NM_001143887.1      AAGGCACACACTGATTTTTTTGAAGCCTTCAAGAATTATGATGAATCTGGAAGTCCAAGA
                    ************************************************************

HsCD00329746        CGAACCACTTGCTTAAAATATTTGGTCTTAGCAAATATGCTTATGAAATCGGGAATAAAT
NM_004236.3         CGAACCACTTGCTTAAAATATTTGGTCTTAGCAAATATGCTTATGAAATCGGGAATAAAT
NM_001143887.1      CGAACCACTTGCTTAAAATATTTGGTCTTAGCAAATATGCTTATGAAATCGGGAATAAAT
                    ************************************************************

HsCD00329746        CCATTTGACTCACAGGAGGCCAAGCCGTACAAAAATGATCCAGAAATTTTAGCAATGACG
NM_004236.3         CCATTTGACTCACAGGAGGCCAAGCCGTACAAAAATGATCCAGAAATTTTAGCAATGACG
NM_001143887.1      CCATTTGACTCACAGGAGGCCAAGCCGTACAAAAATGATCCAGAAATTTTAGCAATGACG
                    ************************************************************

HsCD00329746        AATTTAGTAAGTGCCTATCAGAATAATGACATCACTGAATTTGAAAAGATTCTAAAAACA
NM_004236.3         AATTTAGTAAGTGCCTATCAGAATAATGACATCACTGAATTTGAAAAGATTCTAAAAACA
NM_001143887.1      AATTTAGTAAGTGCCTATCAGAATAATGACATCACTGAATTTGAAAAGATTCTAAAAACA
                    ************************************************************

HsCD00329746        AATCACAGCAACATCATGGATGATCCTTTCATAAGAGAACACATTGAAGAGCTTTTGCGA
NM_004236.3         AATCACAGCAACATCATGGATGATCCTTTCATAAGAGAACACATTGAAGAGCTTTTGCGA
NM_001143887.1      AATCACAGCAACATCATGGATGATCCTTTCATAAGAGAACACATTGAAGAGCTTTTGCGA
                    ************************************************************

HsCD00329746        AACACCAGAACACAAGTGCTTATAAAATTAATTAAGCCTTACACAAGAATACATATTCCT
NM_004236.3         AACATCAGAACACAAGTGCTTATAAAATTAATTAAGCCTTACACAAGAATACATATTCCT
NM_001143887.1      AACATCAGAACACAAGTGCTTATAAAATTAATTAAGCCTTACACAAGAATACATATTCCT
                    **** *******************************************************

HsCD00329746        TTTATTTCTAAGGAGTTAAACATAGATGTAGCTGATGTGGAGAGCTTGCTGGTGCAGTGC
NM_004236.3         TTTATTTCTAAGGAGTTAAACATAGATGTAGCTGATGTGGAGAGCTTGCTGGTGCAGTGC
NM_001143887.1      TTTATTTCTAAGGAGTTAAACATAGATGTAGCTGATGTGGAGAGCTTGCTGGTGCAGTGC
                    ************************************************************

HsCD00329746        ATATTGGATAACACTATTCATGGCCGAATTGATCAAGTCAACCAACTCCTTGAACTGGAT
NM_004236.3         ATATTGGATAACACTATTCATGGCCGAATTGATCAAGTCAACCAACTCCTTGAACTGGAT
NM_001143887.1      ATATTGGATAACACTATTCATGGCCGAATTGATCAAGTCAACCAACTCCTTGAACTGGAT
                    ************************************************************

HsCD00329746        CATCAGAAGAGGGGTGGTGCACGATATACTGCACTAGATAAATGGACCAACCAACTAAAT
NM_004236.3         CATCAGAAGAGGGGTGGTGCACGATATACTGCACTAGATAAATGGACCAACCAACTAAAT
NM_001143887.1      CATCAGAAGAGGGGTGGTGCACGATATACTGCACTAGATAAATGGACCAACCAACTAAAT
                    ************************************************************

HsCD00329746        TCTCTCAACCAGGCTGTAGTCAGTAAACTGGCTTTG
NM_004236.3         TCTCTCAACCAGGCTGTAGTCAGTAAACTGGCTTAA
NM_001143887.1      TCTCTCAACCAGGCTGTAGTCAGTAAACTGGCTTAA
                    **********************************:.

Clone

Cloning Information : 268486
HIP Master Clone ID : 193530
Original Clone ID : FLH268486.01L

Organism


TAX_ID : 9606
Species Specific ID: 9318

Genome

Chromosome : 15
Map Location : 15q21.2
Ensembl : ENSG00000166200

Reagents

Labome : COPS2-antibody