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PKNOX2 (Homo sapiens) in pLenti6.2/V5-DEST (V5-tagged lentiviral mammalian expression vector)

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Explanation of Terms


Gene: Gene Symbol:  PKNOX2
Gene Name:  PBX/knotted 1 homeobox 2
Sequence             
Original Clone ID: FLH268491.01L
Keyword: None
Species: Homo sapiens
Type: cDNA
Vector Name: pLenti6.2/V5-DEST               Format:  FUSION
Source: HIP
Description: None
Comments: None
Mutation/
Discrepancy :
No / No
Publications: None
Authors:
HsCD00329750

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AVAILABLE
No restriction
Special MTA: None

 

Insert sequence: 1416nts         Open reading frame : 1 to 1416
Reference Sequence Alignment

ATGATGCAACATGCCTCCCCAGCCCCCGCTCTGACGATGATGGCCACGCAGAATGTCCCG
CCCCCACCCTACCAGGACAGCCCACAGATGACGGCAACCGCCCAGCCACCCTCCAAGGCC
CAGGCTGTCCACATCTCTGCCCCCTCAGCTGCTGCCAGCACACCTGTGCCCAGTGCCCCC
ATCGACCCCCAGGCCCAGCTGGAGGCTGACAAGCGAGCTGTATACAGGCACCCTCTTTTC
CCGCTCCTGACGCTGCTGTTTGAGAAATGTGAACAGGCCACCCAGGGCTCTGAGTGCATC
ACCTCCGCCAGCTTTGATGTGGACATCGAGAACTTTGTCCACCAGCAGGAACAGGAGCAC
AAACCCTTCTTCAGCGATGACCCAGAACTGGACAATCTGATGGTGAAGGCAATCCAGGTC
CTGAGAATCCACCTGCTGGAGCTGGAGAAAGTCAATGAACTCTGCAAGGACTTTTGTAAC
CGTTACATCACCTGCCTCAAAACCAAGATGCACAGCGACAACCTGCTCAGGAATGATCTA
GGGGGGCCCTACTCCCCCAACCAGCCCTCCATCAACCTTCACTCCCAGGACCTCCTGCAG
AATTCCCCCAATTCCATGTCCGGAGTCTCCAATAACCCCCAGGGGATTGTGGTCCCAGCC
TCAGCGCTCCAGCAGGGCAACATCGCCATGACAACCGTCAACTCACAAGTTGTGTCAGGT
GGAGCCTTATACCAACCGGTTACCATGGTAACCTCCCAGGGTCAGGTGGTCACCCAAGCA
ATCCCCCAGGGAGCCATCCAGATCCAGAACACACAGGTTAACCTTGACCTCACCTCCCTC
CTGGACAATGAGGATAAGAAGTCCAAGAACAAACGAGGAGTCTTGCCCAAGCATGCCACC
AATATAATGCGTTCTTGGCTCTTCCAGCATCTCATGCACCCCTACCCCACGGAGGATGAG
AAGAGGCAGATCGCAGCCCAGACCAACCTCACCCTCCTGCAAGTAAACAACTGGTTCATC
AATGCCCGGAGGCGCATCCTGCAGCCCATGCTTGATGCCAGCAACCCAGATCCTGCCCCC
AAAGCCAAGAAGATCAAGTCTCAGCACCGGCCCACCCAAAGATTCTGGCCCAACTCCATC
GCTGCGGGGGTGCTGCAGCAGCAGGGCGGTGCCCCAGGGACAAACCCCGATGGTTCCATC
AACTTGGACAACCTGCAGTCCCTGTCCTCAGACAGTGCCACCATGGCCATGCAGCAGGCT
ATGATGGCTGCACACGATGACTCATTGGATGGGACAGAAGAAGAGGATGAGGATGAGATG
GAAGAGGAGGAGGAGGAGCTGGAGGAGGAGGTCGACGAGCTGCAGACGACAAATGTCAGC
GACCTGGGCTTGGAACACAGTGACTCCCTGGAGTTG


Coding Sequence Details
Insert Sequence Verified?: N Verification Method: Insert was fully sequenced in parent vector
Reference Sequence Annotations:
End on reference sequence 1687
Start on reference sequence 272
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
5p GTACAAAAAAGCAGGCTCCACC(atg-goi)

3' Linker Sequence:
5p (goi-stop)GACCCAGCTTTCTTGTAC

 

 Recommended Growth Condition:

Distributed in bacterial strain : DH5-alpha T1 phage resistant
Antibiotic Selection: Host Type:  bacterial    Marker:  ampicillin
Host Type:  mammalian    Marker:  blasticidin
Bacterial Selection Condition:  100 ug/mL ampicillin
Growth Condition:  Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:  Commonly used conditions for ampicillin resistant plasmid clones.
Protein Expression Results: None
Recommended expression in: Not Applicable
       DyNA Vector Map

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Vector Name: pLenti6.2/V5-DEST

pLenti6.2V5-DEST in advanced viewed

Synonyms: pLenti6.2/V5-DEST
Sequencing Primer: Forward:  CMV forward
Reverse:  V5 reverse
Description: Lentiviral mammalian expression vector;CMV promoter and C-terminal V5 tag; ampicillin resistance in bacteria; blasticidin resistance in mammalian cells; Gateway recombinational cloning.
Comments: None
Size (bp): 8813
Parent Vector: None
Empty Vector: None
Properties: Gateway, acceptor (destination), lentiviral infection, mammalian expression, recombinational cloning, with tag/fusion/marker
Author Name: Invitrogen
Publications: None
Vector Map:         Vector Sequence:
Sequence:

 



Vector Features:

Type Name Description Start Position End Position
bacterial origin pUC ori pUC origin of replication 7733 8406
death cassette ccdB ccdB death cassette (removed when insert is present) 3767 3976
packaging signal HIV-1 psi HIV-1 psi packaging signal 521 565
poly-A signal SV40 SV40 polyadenylation signal 5639 5770
promoter RSV RSV/5' LTR hybrid promoter 1 410
promoter CMV CMV promoter driving gene of interest 1809 2392
promoter PGK PGK promoter to drive selection marker in mammalian cells 4266 4771
recombination site attR2 attR2 recombinational cloning site 4127 4142
recombination site attR1 attR1 recombinational cloning site 2440 2456
response element HIV-1 RRE HIV-1 Rev response element (RRE) 1075 1308
selectable marker AmpR Ampicillin resistance gene for selection in bacteria 6728 7588
selectable marker BlastR Blasticidin resistance gene in mammalian cells 4849 5249
selectable marker CmR chloramphenicol resistance marker (removed in plasmids with insert) 2673 3329
splice acceptor site 3' splice acceptor two 3' splice acceptors at 1656 and 1684 1656 1656
splice donor site 5' splice donor 5' splice donor 520 520
tag V5 C-terminal V5 epitope tag 4197 4238

 

  • Gene
  • Protein
  • Clone
  • Organism
  • Genome
  • Reagents

Gene

 

Gene Symbol : PKNOX2
Symbol Nomenclature : PKNOX2
SYNONYM : PREP2
Designation : PBX/knotted homeobox 2|homeobox protein PKNOX2|homeobox protein PREP-2
Full Nomenclature : PBX/knotted 1 homeobox 2
GENEID : 63876
HGNC : 16714
MIM : 613066
Vega : OTTHUMG00000165884
Target GenBank: BC045626

 

Reference Sequence Alignment

  

HsCD00329750        ATGATGCAACATGCCTCCCCAGCCCCCGCTCTGACGATGATGGCCACGCAGAATGTCCCG
XM_005271642.1      ATGATGCAACATGCCTCCCCAGCCCCCGCTCTGACGATGATGGCCACGCAGAATGTCCCG
XM_005271643.1      ------------------------------------------------------------
NM_022062.2         ATGATGCAACATGCCTCCCCAGCCCCCGCTCTGACGATGATGGCCACGCAGAATGTCCCG
                                                                                

HsCD00329750        CCCCCACCCTACCAGGACAGCCCACAGATGACGGCAACCGCCCAGCCACCCTCCAAGGCC
XM_005271642.1      CCCCCACCCTACCAGGACAGCCCACAGATGACGGCAACCGCCCAGCCACCCTCCAAGGCC
XM_005271643.1      ---------------------------ATGACGGCAACCGCCCAGCCACCCTCCAAGGCC
NM_022062.2         CCCCCACCCTACCAGGACAGCCCACAGATGACGGCAACCGCCCAGCCACCCTCCAAGGCC
                                               *********************************

HsCD00329750        CAGGCTGTCCACATCTCTGCCCCCTCAGCTGCTGCCAGCACACCTGTGCCCAGTGCCCCC
XM_005271642.1      CAGGCTGTCCACATCTCTGCCCCCTCAGCTGCTGCCAGCACACCTGTGCCCAGTGCCCCC
XM_005271643.1      CAGGCTGTCCACATCTCTGCCCCCTCAGCTGCTGCCAGCACACCTGTGCCCAGTGCCCCC
NM_022062.2         CAGGCTGTCCACATCTCTGCCCCCTCAGCTGCTGCCAGCACACCTGTGCCCAGTGCCCCC
                    ************************************************************

HsCD00329750        ATCGACCCCCAGGCCCAGCTGGAGGCTGACAAGCGAGCTGTATACAGGCACCCTCTTTTC
XM_005271642.1      ATCGACCCCCAGGCCCAGCTGGAGGCTGACAAGCGAGCTGTATACAGGCACCCTCTTTTC
XM_005271643.1      ATCGACCCCCAGGCCCAGCTGGAGGCTGACAAGCGAGCTGTATACAGGCACCCTCTTTTC
NM_022062.2         ATCGACCCCCAGGCCCAGCTGGAGGCTGACAAGCGAGCTGTATACAGGCACCCTCTTTTC
                    ************************************************************

HsCD00329750        CCGCTCCTGACGCTGCTGTTTGAGAAATGTGAACAGGCCACCCAGGGCTCTGAGTGCATC
XM_005271642.1      CCGCTCCTGACGCTGCTGTTTGAGAAATGTGAACAGGCCACCCAGGGCTCTGAGTGCATC
XM_005271643.1      CCGCTCCTGACGCTGCTGTTTGAGAAATGTGAACAGGCCACCCAGGGCTCTGAGTGCATC
NM_022062.2         CCGCTCCTGACGCTGCTGTTTGAGAAATGTGAACAGGCCACCCAGGGCTCTGAGTGCATC
                    ************************************************************

HsCD00329750        ACCTCCGCCAGCTTTGATGTGGACATCGAGAACTTTGTCCACCAGCAGGAACAGGAGCAC
XM_005271642.1      ACCTCCGCCAGCTTTGATGTGGACATCGAGAACTTTGTCCACCAGCAGGAACAGGAGCAC
XM_005271643.1      ACCTCCGCCAGCTTTGATGTGGACATCGAGAACTTTGTCCACCAGCAGGAACAGGAGCAC
NM_022062.2         ACCTCCGCCAGCTTTGATGTGGACATCGAGAACTTTGTCCACCAGCAGGAACAGGAGCAC
                    ************************************************************

HsCD00329750        AAACCCTTCTTCAGCGATGACCCAGAACTGGACAATCTGATGGTGAAGGCAATCCAGGTC
XM_005271642.1      AAACCCTTCTTCAGCGATGACCCAGAACTGGACAATCTGATGGTGAAGGCAATCCAGGTC
XM_005271643.1      AAACCCTTCTTCAGCGATGACCCAGAACTGGACAATCTGATGGTGAAGGCAATCCAGGTC
NM_022062.2         AAACCCTTCTTCAGCGATGACCCAGAACTGGACAATCTGATGGTGAAGGCAATCCAGGTC
                    ************************************************************

HsCD00329750        CTGAGAATCCACCTGCTGGAGCTGGAGAAAGTCAATGAACTCTGCAAGGACTTTTGTAAC
XM_005271642.1      CTGAGAATCCACCTGCTGGAGCTGGAGAAAGTCAATGAACTCTGCAAGGACTTTTGTAAC
XM_005271643.1      CTGAGAATCCACCTGCTGGAGCTGGAGAAAGTCAATGAACTCTGCAAGGACTTTTGTAAC
NM_022062.2         CTGAGAATCCACCTGCTGGAGCTGGAGAAAGTCAATGAACTCTGCAAGGACTTTTGTAAC
                    ************************************************************

HsCD00329750        CGTTACATCACCTGCCTCAAAACCAAGATGCACAGCGACAACCTGCTCAGGAATGATCTA
XM_005271642.1      CGTTACATCACCTGCCTCAAAACCAAGATGCACAGCGACAACCTGCTCAGGAATGATCTA
XM_005271643.1      CGTTACATCACCTGCCTCAAAACCAAGATGCACAGCGACAACCTGCTCAGGAATGATCTA
NM_022062.2         CGTTACATCACCTGCCTCAAAACCAAGATGCACAGCGACAACCTGCTCAGGAATGATCTA
                    ************************************************************

HsCD00329750        GGGGGGCCCTACTCCCCCAACCAGCCCTCCATCAACCTTCACTCCCAGGACCTCCTGCAG
XM_005271642.1      GGGGGGCCCTACTCCCCCAACCAGCCCTCCATCAACCTTCACTCACAGGACCTCCTGCAG
XM_005271643.1      GGGGGGCCCTACTCCCCCAACCAGCCCTCCATCAACCTTCACTCACAGGACCTCCTGCAG
NM_022062.2         GGGGGGCCCTACTCCCCCAACCAGCCCTCCATCAACCTTCACTCACAGGACCTCCTGCAG
                    ********************************************.***************

HsCD00329750        AATTCCCCCAATTCCATGTCCGGAGTCTCCAATAACCCCCAGGGGATTGTGGTCCCAGCC
XM_005271642.1      AATTCCCCCAATTCCATGTCCGGAGTCTCCAATAACCCCCAGGGGATTGTGGTCCCAGCC
XM_005271643.1      AATTCCCCCAATTCCATGTCCGGAGTCTCCAATAACCCCCAGGGGATTGTGGTCCCAGCC
NM_022062.2         AATTCCCCCAATTCCATGTCCGGAGTCTCCAATAACCCCCAGGGGATTGTGGTCCCAGCC
                    ************************************************************

HsCD00329750        TCAGCGCTCCAGCAGGGCAACATCGCCATGACAACCGTCAACTCACAAGTTGTGTCAGGT
XM_005271642.1      TCAGCGCTCCAGCAGGGCAACATCGCCATGACAACCGTCAACTCACAAGTTGTGTCAGGT
XM_005271643.1      TCAGCGCTCCAGCAGGGCAACATCGCCATGACAACCGTCAACTCACAAGTTGTGTCAGGT
NM_022062.2         TCAGCGCTCCAGCAGGGCAACATCGCCATGACAACCGTCAACTCACAAGTTGTGTCAGGT
                    ************************************************************

HsCD00329750        GGAGCCTTATACCAACCGGTTACCATGGTAACCTCCCAGGGTCAGGTGGTCACCCAAGCA
XM_005271642.1      GGAGCCTTATACCAACCGGTTACCATGGTAACCTCCCAGGGTCAGGTGGTCACCCAAGCA
XM_005271643.1      GGAGCCTTATACCAACCGGTTACCATGGTAACCTCCCAGGGTCAGGTGGTCACCCAAGCA
NM_022062.2         GGAGCCTTATACCAACCGGTTACCATGGTAACCTCCCAGGGTCAGGTGGTCACCCAAGCA
                    ************************************************************

HsCD00329750        ATCCCCCAGGGAGCCATCCAGATCCAGAACACACAGGTTAACCTTGACCTCACCTCCCTC
XM_005271642.1      ATCCCCCAGGGAGCCATCCAGATCCAGAACACACAGGTTAACCTTGACCTCACCTCCCTC
XM_005271643.1      ATCCCCCAGGGAGCCATCCAGATCCAGAACACACAGGTTAACCTTGACCTCACCTCCCTC
NM_022062.2         ATCCCCCAGGGAGCCATCCAGATCCAGAACACACAGGTTAACCTTGACCTCACCTCCCTC
                    ************************************************************

HsCD00329750        CTGGACAATGAGGATAAGAAGTCCAAGAACAAACGAGGAGTCTTGCCCAAGCATGCCACC
XM_005271642.1      CTGGACAATGAGGATAAGAAGTCCAAGAACAAACGAGGAGTCTTGCCCAAGCATGCCACC
XM_005271643.1      CTGGACAATGAGGATAAGAAGTCCAAGAACAAACGAGGAGTCTTGCCCAAGCATGCCACC
NM_022062.2         CTGGACAATGAGGATAAGAAGTCCAAGAACAAACGAGGAGTCTTGCCCAAGCATGCCACC
                    ************************************************************

HsCD00329750        AATATAATGCGTTCTTGGCTCTTCCAGCATCTCATGCACCCCTACCCCACGGAGGATGAG
XM_005271642.1      AATATAATGCGTTCTTGGCTCTTCCAGCATCTCATGCACCCCTACCCCACGGAGGATGAG
XM_005271643.1      AATATAATGCGTTCTTGGCTCTTCCAGCATCTCATGCACCCCTACCCCACGGAGGATGAG
NM_022062.2         AATATAATGCGTTCTTGGCTCTTCCAGCATCTCATGCACCCCTACCCCACGGAGGATGAG
                    ************************************************************

HsCD00329750        AAGAGGCAGATCGCAGCCCAGACCAACCTCACCCTCCTGCAAGTAAACAACTGGTTCATC
XM_005271642.1      AAGAGGCAGATCGCAGCCCAGACCAACCTCACCCTCCTGCAAGTAAACAACTGGTTCATC
XM_005271643.1      AAGAGGCAGATCGCAGCCCAGACCAACCTCACCCTCCTGCAAGTAAACAACTGGTTCATC
NM_022062.2         AAGAGGCAGATCGCAGCCCAGACCAACCTCACCCTCCTGCAAGTAAACAACTGGTTCATC
                    ************************************************************

HsCD00329750        AATGCCCGGAGGCGCATCCTGCAGCCCATGCTTGATGCCAGCAACCCAGATCCTGCCCCC
XM_005271642.1      AATGCCCGGAGGCGCATCCTGCAGCCCATGCTTGATGCCAGCAACCCAGATCCTGCCCCC
XM_005271643.1      AATGCCCGGAGGCGCATCCTGCAGCCCATGCTTGATGCCAGCAACCCAGATCCTGCCCCC
NM_022062.2         AATGCCCGGAGGCGCATCCTGCAGCCCATGCTTGATGCCAGCAACCCAGATCCTGCCCCC
                    ************************************************************

HsCD00329750        AAAGCCAAGAAGATCAAGTCTCAGCACCGGCCCACCCAAAGATTCTGGCCCAACTCCATC
XM_005271642.1      AAAGCCAAGAAGATCAAGTCTCAGCACCGGCCCACCCAAAGATTCTGGCCCAACTCCATC
XM_005271643.1      AAAGCCAAGAAGATCAAGTCTCAGCACCGGCCCACCCAAAGATTCTGGCCCAACTCCATC
NM_022062.2         AAAGCCAAGAAGATCAAGTCTCAGCACCGGCCCACCCAAAGATTCTGGCCCAACTCCATC
                    ************************************************************

HsCD00329750        GCTGCGGGGGTGCTGCAGCAGCAGGGCGGTGCCCCAGGGACAAACCCCGATGGTTCCATC
XM_005271642.1      GCTGCGGGGGTGCTGCAGCAGCAGGGCGGTGCCCCAGGGACAAACCCCGATGGTTCCATC
XM_005271643.1      GCTGCGGGGGTGCTGCAGCAGCAGGGCGGTGCCCCAGGGACAAACCCCGATGGTTCCATC
NM_022062.2         GCTGCGGGGGTGCTGCAGCAGCAGGGCGGTGCCCCAGGGACAAACCCCGATGGTTCCATC
                    ************************************************************

HsCD00329750        AACTTGGACAACCTGCAGTCCCTGTCCTCAGACAGTGCCACCATGGCCATGCAGCAGGCT
XM_005271642.1      AACTTGGACAACCTGCAGTCCCTGTCCTCAGACAGTGCCACCATGGCCATGCAGCAGGCT
XM_005271643.1      AACTTGGACAACCTGCAGTCCCTGTCCTCAGACAGTGCCACCATGGCCATGCAGCAGGCT
NM_022062.2         AACTTGGACAACCTGCAGTCCCTGTCCTCAGACAGTGCCACCATGGCCATGCAGCAGGCT
                    ************************************************************

HsCD00329750        ATGATGGCTGCACACGATGACTCATTGGATGGGACAGAAGAAGAGGATGAGGATGAGATG
XM_005271642.1      ATGATGGCTGCACACGATGACTCATTGGATGGGACAGAAGAAGAGGATGAGGATGAGATG
XM_005271643.1      ATGATGGCTGCACACGATGACTCATTGGATGGGACAGAAGAAGAGGATGAGGATGAGATG
NM_022062.2         ATGATGGCTGCACACGATGACTCATTGGATGGGACAGAAGAAGAGGATGAGGATGAGATG
                    ************************************************************

HsCD00329750        GAAGAG---GAGGAGGAGGAGCTGGAGGAGGAGGTCGACGAGCTGCAGACGACAAATGTC
XM_005271642.1      GAAGAGGAGGAGGAGGAGGAGCTGGAGGAGGAGGTCGACGAGCTGCAGACGACAAATGTC
XM_005271643.1      GAAGAGGAGGAGGAGGAGGAGCTGGAGGAGGAGGTCGACGAGCTGCAGACGACAAATGTC
NM_022062.2         GAAGAGGAGGAGGAGGAGGAGCTGGAGGAGGAGGTCGACGAGCTGCAGACGACAAATGTC
                    ******   ***************************************************

HsCD00329750        AGCGACCTGGGCTTGGAACACAGTGACTCCCTGGAGTTG
XM_005271642.1      AGCGACCTGGGCTTGGAACACAGTGACTCCCTGGAGTAG
XM_005271643.1      AGCGACCTGGGCTTGGAACACAGTGACTCCCTGGAGTAG
NM_022062.2         AGCGACCTGGGCTTGGAACACAGTGACTCCCTGGAGTAG
                    *************************************:*

Protein

SMART domain : HOX : Homeodomain
UniProt : Q96KN3
HPRD : 17857

Clone

Cloning Information : 268491
HIP Master Clone ID : 193538
Original Clone ID : FLH268491.01L

Organism


TAX_ID : 9606
Species Specific ID: 63876

Genome

Chromosome : 11
Map Location : 11q24.2
Ensembl : ENSG00000165495

Reagents

Labome : PREP2-antibody